Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013754.01 | MsG0480023527.01 | 0.801170 | 1.021692e-48 | 4.846382e-46 |
| MsG0480021465.01 | MsG0480023527.01 | 0.804167 | 2.459920e-49 | 1.259525e-46 |
| MsG0280007815.01 | MsG0480023527.01 | 0.801638 | 8.191088e-49 | 3.931521e-46 |
| MsG0280010233.01 | MsG0480023527.01 | 0.808146 | 3.574328e-50 | 2.028138e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023527.01.T01 | MTR_8g095210 | 75.610 | 41 | 7 | 1 | 20 | 57 | 642 | 682 | 4.82e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCACTTGTTAACATATTTG+AGG | 0.381698 | 4:-86481525 | None:intergenic |
| CTCTGGTTGACTATAGAAAC+AGG | 0.436118 | 4:+86481088 | MsG0480023527.01.T01:CDS |
| ATTCACAACAACTATTGAAA+AGG | 0.439020 | 4:-86481785 | None:intergenic |
| GGGAAGCTACAATATGAGTA+TGG | 0.441691 | 4:+86481865 | MsG0480023527.01.T01:CDS |
| ATGTGATGCGCATTGATTGT+TGG | 0.476292 | 4:+86481806 | MsG0480023527.01.T01:CDS |
| TAGAATTTACCCTCATCCTC+TGG | 0.493432 | 4:+86481071 | MsG0480023527.01.T01:CDS |
| CTATAGTCAACCAGAGGATG+AGG | 0.499274 | 4:-86481081 | None:intergenic |
| ATGACGAGTCTGTGCTTCGA+AGG | 0.520616 | 4:+86481456 | MsG0480023527.01.T01:CDS |
| TCCTCAAATATGTTAACAAG+TGG | 0.527237 | 4:+86481524 | MsG0480023527.01.T01:CDS |
| TGTAGTGGAGACCCTTCGAC+TGG | 0.530760 | 4:+86481843 | MsG0480023527.01.T01:CDS |
| AAGAAGATTACAATGGAACT+TGG | 0.532216 | 4:+86481547 | MsG0480023527.01.T01:CDS |
| AACATATTTGAGGAAAGCAC+TGG | 0.552278 | 4:-86481515 | None:intergenic |
| ATTGTAGCTTCCCCAGTCGA+AGG | 0.568977 | 4:-86481855 | None:intergenic |
| GTAGTGGAGACCCTTCGACT+GGG | 0.587447 | 4:+86481844 | MsG0480023527.01.T01:CDS |
| GTTGTGTGCTGATATTGTAG+TGG | 0.594620 | 4:+86481828 | MsG0480023527.01.T01:CDS |
| TCGTGTTCGAGCTCCACCTA+GGG | 0.599113 | 4:+86481484 | MsG0480023527.01.T01:CDS |
| CAGAACTGCAGAAAGATCAA+AGG | 0.604239 | 4:+86481412 | MsG0480023527.01.T01:intron |
| TTGTAGCTTCCCCAGTCGAA+GGG | 0.618968 | 4:-86481854 | None:intergenic |
| CTCGTGTTCGAGCTCCACCT+AGG | 0.625716 | 4:+86481483 | MsG0480023527.01.T01:CDS |
| TAGTGGAGACCCTTCGACTG+GGG | 0.635538 | 4:+86481845 | MsG0480023527.01.T01:CDS |
| TATAGTCAACCAGAGGATGA+GGG | 0.645696 | 4:-86481080 | None:intergenic |
| TAGAAACATAAGCAATCAGG+GGG | 0.657037 | 4:-86481896 | None:intergenic |
| CTGTTTCTATAGTCAACCAG+AGG | 0.703527 | 4:-86481087 | None:intergenic |
| CAAGTGGAAGAAGATTACAA+TGG | 0.704466 | 4:+86481540 | MsG0480023527.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAATAAAGTGAATATAG+AGG | - | Chr4:86481318-86481337 | None:intergenic | 15.0% |
| !! | ATTAATAAAGTGAATATAGA+GGG | - | Chr4:86481317-86481336 | None:intergenic | 15.0% |
| !!! | AGATTTTATCAAAAAAAAGT+TGG | + | Chr4:86481212-86481231 | MsG0480023527.01.T01:intron | 15.0% |
| !!! | TTTATAAAACATGTTTTTCT+AGG | + | Chr4:86481727-86481746 | MsG0480023527.01.T01:intron | 15.0% |
| !! | TAGCTTATTTAACAAACATA+TGG | + | Chr4:86481258-86481277 | MsG0480023527.01.T01:intron | 20.0% |
| ! | ATTCACAACAACTATTGAAA+AGG | - | Chr4:86481788-86481807 | None:intergenic | 25.0% |
| !! | ACTAATTTTATAGTGTGTAG+AGG | + | Chr4:86481137-86481156 | MsG0480023527.01.T01:intron | 25.0% |
| !! | TAAAACATGTTTTTCTAGGT+AGG | + | Chr4:86481731-86481750 | MsG0480023527.01.T01:intron | 25.0% |
| AAAAAATCTCAGAAGTCAAG+GGG | - | Chr4:86481363-86481382 | None:intergenic | 30.0% | |
| AAGAAGATTACAATGGAACT+TGG | + | Chr4:86481547-86481566 | MsG0480023527.01.T01:CDS | 30.0% | |
| ACAAAAAATCTCAGAAGTCA+AGG | - | Chr4:86481365-86481384 | None:intergenic | 30.0% | |
| CAAAAAATCTCAGAAGTCAA+GGG | - | Chr4:86481364-86481383 | None:intergenic | 30.0% | |
| TCCTCAAATATGTTAACAAG+TGG | + | Chr4:86481524-86481543 | MsG0480023527.01.T01:CDS | 30.0% | |
| TTTCTACCATGATTGAAGAA+TGG | - | Chr4:86481681-86481700 | None:intergenic | 30.0% | |
| ! | AATTTTATAGTGTGTAGAGG+AGG | + | Chr4:86481140-86481159 | MsG0480023527.01.T01:intron | 30.0% |
| ! | ATTTTATAGTGTGTAGAGGA+GGG | + | Chr4:86481141-86481160 | MsG0480023527.01.T01:intron | 30.0% |
| ! | TCCACTTGTTAACATATTTG+AGG | - | Chr4:86481528-86481547 | None:intergenic | 30.0% |
| !! | ATAGCGTTGAATGTAGTTTT+TGG | + | Chr4:86481594-86481613 | MsG0480023527.01.T01:intron | 30.0% |
| !! | TGTAGTTTTTGGATCCATTT+TGG | + | Chr4:86481605-86481624 | MsG0480023527.01.T01:intron | 30.0% |
| AAAAATCTCAGAAGTCAAGG+GGG | - | Chr4:86481362-86481381 | None:intergenic | 35.0% | |
| CAAGTGGAAGAAGATTACAA+TGG | + | Chr4:86481540-86481559 | MsG0480023527.01.T01:CDS | 35.0% | |
| ! | AACATATTTGAGGAAAGCAC+TGG | - | Chr4:86481518-86481537 | None:intergenic | 35.0% |
| ! | CATTTTGGATGATGTACAAG+CGG | + | Chr4:86481620-86481639 | MsG0480023527.01.T01:intron | 35.0% |
| CAGAACTGCAGAAAGATCAA+AGG | + | Chr4:86481412-86481431 | MsG0480023527.01.T01:intron | 40.0% | |
| CGCTTGTACATCATCCAAAA+TGG | - | Chr4:86481622-86481641 | None:intergenic | 40.0% | |
| CTCTGGTTGACTATAGAAAC+AGG | + | Chr4:86481088-86481107 | MsG0480023527.01.T01:CDS | 40.0% | |
| CTTGCACCATTCTTCAATCA+TGG | + | Chr4:86481672-86481691 | MsG0480023527.01.T01:intron | 40.0% | |
| GGGAAGCTACAATATGAGTA+TGG | + | Chr4:86481865-86481884 | MsG0480023527.01.T01:CDS | 40.0% | |
| TAGAATTTACCCTCATCCTC+TGG | + | Chr4:86481071-86481090 | MsG0480023527.01.T01:CDS | 40.0% | |
| TATAGTCAACCAGAGGATGA+GGG | - | Chr4:86481083-86481102 | None:intergenic | 40.0% | |
| ! | GTTGTGTGCTGATATTGTAG+TGG | + | Chr4:86481828-86481847 | MsG0480023527.01.T01:CDS | 40.0% |
| !! | ATGTGATGCGCATTGATTGT+TGG | + | Chr4:86481806-86481825 | MsG0480023527.01.T01:CDS | 40.0% |
| !! | CTGTTTCTATAGTCAACCAG+AGG | - | Chr4:86481090-86481109 | None:intergenic | 40.0% |
| CTATAGTCAACCAGAGGATG+AGG | - | Chr4:86481084-86481103 | None:intergenic | 45.0% | |
| !!! | GTTTTATAAAAAAAAAAAAA+AGG | - | Chr4:86481717-86481736 | None:intergenic | 5.0% |
| ATGACGAGTCTGTGCTTCGA+AGG | + | Chr4:86481456-86481475 | MsG0480023527.01.T01:CDS | 50.0% | |
| ATTGTAGCTTCCCCAGTCGA+AGG | - | Chr4:86481858-86481877 | None:intergenic | 50.0% | |
| TTGTAGCTTCCCCAGTCGAA+GGG | - | Chr4:86481857-86481876 | None:intergenic | 50.0% | |
| ! | ACTGGCAGATTTTCCCTAGG+TGG | - | Chr4:86481500-86481519 | None:intergenic | 50.0% |
| !!! | AGCACTGGCAGATTTTCCCT+AGG | - | Chr4:86481503-86481522 | None:intergenic | 50.0% |
| TAGTGGAGACCCTTCGACTG+GGG | + | Chr4:86481845-86481864 | MsG0480023527.01.T01:CDS | 55.0% | |
| TCGTGTTCGAGCTCCACCTA+GGG | + | Chr4:86481484-86481503 | MsG0480023527.01.T01:CDS | 55.0% | |
| ! | GTAGTGGAGACCCTTCGACT+GGG | + | Chr4:86481844-86481863 | MsG0480023527.01.T01:CDS | 55.0% |
| ! | TGTAGTGGAGACCCTTCGAC+TGG | + | Chr4:86481843-86481862 | MsG0480023527.01.T01:CDS | 55.0% |
| CTCGTGTTCGAGCTCCACCT+AGG | + | Chr4:86481483-86481502 | MsG0480023527.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 86481036 | 86481903 | 86481036 | ID=MsG0480023527.01;Name=MsG0480023527.01 |
| Chr4 | mRNA | 86481036 | 86481903 | 86481036 | ID=MsG0480023527.01.T01;Parent=MsG0480023527.01;Name=MsG0480023527.01.T01;_AED=0.89;_eAED=0.89;_QI=0|0|0|0.33|0.5|0.66|3|0|139 |
| Chr4 | exon | 86481036 | 86481109 | 86481036 | ID=MsG0480023527.01.T01:exon:3038;Parent=MsG0480023527.01.T01 |
| Chr4 | exon | 86481415 | 86481605 | 86481415 | ID=MsG0480023527.01.T01:exon:3039;Parent=MsG0480023527.01.T01 |
| Chr4 | exon | 86481749 | 86481903 | 86481749 | ID=MsG0480023527.01.T01:exon:3040;Parent=MsG0480023527.01.T01 |
| Chr4 | CDS | 86481036 | 86481109 | 86481036 | ID=MsG0480023527.01.T01:cds;Parent=MsG0480023527.01.T01 |
| Chr4 | CDS | 86481415 | 86481605 | 86481415 | ID=MsG0480023527.01.T01:cds;Parent=MsG0480023527.01.T01 |
| Chr4 | CDS | 86481749 | 86481903 | 86481749 | ID=MsG0480023527.01.T01:cds;Parent=MsG0480023527.01.T01 |
| Gene Sequence |
| Protein sequence |
