AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024079.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024079.01.T01 MTR_7g106180 59.036 166 68 0 60 225 4 169 1.53e-71 220
MsG0580024079.01.T01 MTR_7g406910 48.905 137 70 0 49 185 78 214 1.74e-45 152
MsG0580024079.01.T01 MTR_3g106040 56.897 116 47 2 38 150 65 180 2.51e-41 141
MsG0580024079.01.T01 MTR_0010s0370 23.776 143 108 1 82 223 34 176 8.45e-13 65.9
MsG0580024079.01.T01 MTR_0010s0350 23.776 143 108 1 82 223 34 176 8.45e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024079.01.T01 AT3G31430 23.301 206 152 3 58 262 111 311 3.37e-15 75.1
MsG0580024079.01.T01 AT2G01050 23.786 206 148 6 59 262 58 256 1.45e-13 70.9
MsG0580024079.01.T01 AT5G36228 21.635 208 160 2 56 262 13 218 1.77e-11 64.3

Find 82 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCATTAGTAGACTGGATT+TGG 0.231938 5:+564211 None:intergenic
TTCAAAATTTCAGAAATAAA+AGG 0.234273 5:-564681 MsG0580024079.01.T01:CDS
CTTCATTGTGAGATTCTTGA+TGG 0.290480 5:+564868 None:intergenic
TGCATTAGTAGACTGGATTT+GGG 0.331618 5:+564212 None:intergenic
TGTGAGATTCTTGATGGTTA+TGG 0.343849 5:+564874 None:intergenic
AAGATATCAATAGAGCTCTA+AGG 0.351949 5:-564626 MsG0580024079.01.T01:CDS
ATGAGGTAGATGGAATTAAC+TGG 0.361474 5:-564323 MsG0580024079.01.T01:CDS
GAAGTGGCAAGCCCCATAAT+TGG 0.367177 5:+564502 None:intergenic
ATCCTCCACAGTTCCTTAAC+AGG 0.379421 5:-564726 MsG0580024079.01.T01:CDS
GGAATCTTGATTTCCAAGAT+TGG 0.395179 5:+564911 None:intergenic
ACAGTTCCTTAACAGGTATC+TGG 0.397721 5:-564719 MsG0580024079.01.T01:CDS
ATCCCACAACAGCCCAATCT+TGG 0.399474 5:-564924 MsG0580024079.01.T01:CDS
TGTTGCCAATGATGCTGTTC+TGG 0.405039 5:+564778 None:intergenic
GGTTATGGAATTGAGGGGTT+GGG 0.406710 5:+564889 None:intergenic
GAATCTTGATTTCCAAGATT+GGG 0.410294 5:+564912 None:intergenic
CACTGTAAATCTATAGCTAT+GGG 0.424327 5:-564480 MsG0580024079.01.T01:CDS
GCTTGAAGGATGAACTTTGC+CGG 0.425992 5:-566351 MsG0580024079.01.T01:CDS
TCCTGCCAGAACAGCATCAT+TGG 0.426387 5:-564783 MsG0580024079.01.T01:CDS
GTTATGGAATTGAGGGGTTG+GGG 0.433477 5:+564890 None:intergenic
CTTGATGGTTATGGAATTGA+GGG 0.434386 5:+564883 None:intergenic
GATCGGAGTCAGGCTCCTCT+TGG 0.448148 5:+564177 None:intergenic
TCTTGATGGTTATGGAATTG+AGG 0.451825 5:+564882 None:intergenic
CAAATCCAGTCTACTAATGC+AGG 0.452131 5:-564210 MsG0580024079.01.T01:CDS
GGGGAAGTCCATGGATCATA+AGG 0.455888 5:-564605 MsG0580024079.01.T01:CDS
TACATAGGTAATGAGGTAGA+TGG 0.464628 5:-564333 MsG0580024079.01.T01:CDS
TCACTGTAAATCTATAGCTA+TGG 0.465419 5:-564481 MsG0580024079.01.T01:CDS
TTTCCAAGATTGGGCTGTTG+TGG 0.470555 5:+564921 None:intergenic
CAGTAGCATGTGGAGCTTGA+AGG 0.474576 5:-566365 None:intergenic
GTATCAAATTTCTATGGAAA+AGG 0.474856 5:-564652 MsG0580024079.01.T01:CDS
AGACTGGATTTGGGGAGAAG+TGG 0.478681 5:+564221 None:intergenic
GAACTGTGGAGGATAGAGAT+GGG 0.482673 5:+564735 None:intergenic
TCATTCGTACGGATCTTAAT+CGG 0.483307 5:+566284 None:intergenic
TTCCAAGATTGGGCTGTTGT+GGG 0.484351 5:+564922 None:intergenic
TATGTAGATTCCTGCTCTGA+TGG 0.484486 5:+564350 None:intergenic
GCCAATGATGCTGTTCTGGC+AGG 0.487230 5:+564782 None:intergenic
AGATATCAATAGAGCTCTAA+GGG 0.487866 5:-564625 MsG0580024079.01.T01:CDS
TGGTTATGGAATTGAGGGGT+TGG 0.494180 5:+564888 None:intergenic
CATGGATCATAAGGAACTGC+TGG 0.497518 5:-564596 MsG0580024079.01.T01:CDS
TTTCTTGATAGTAACAGAAT+GGG 0.500971 5:+564829 None:intergenic
ATTACACCAGATACCTGTTA+AGG 0.502108 5:+564713 None:intergenic
AGGATGAACTTTGCCGGAGA+AGG 0.503747 5:-566345 MsG0580024079.01.T01:CDS
GGATTTACTTGTCTGTCCCA+TGG 0.509883 5:+564561 None:intergenic
GTTTCTTGATAGTAACAGAA+TGG 0.510849 5:+564828 None:intergenic
GATTCCTGCTCTGATGGGAT+TGG 0.513638 5:+564356 None:intergenic
GCCCCATAATTGGATCCAAA+GGG 0.524819 5:+564512 None:intergenic
GGAACTGTGGAGGATAGAGA+TGG 0.525211 5:+564734 None:intergenic
ATGTAGATTCCTGCTCTGAT+GGG 0.536669 5:+564351 None:intergenic
GATCATAAGGAACTGCTGGC+TGG 0.538378 5:-564592 MsG0580024079.01.T01:CDS
TCATCAAAACCATCCTTCTC+CGG 0.538620 5:+566332 None:intergenic
CCCCTTTGGATCCAATTATG+GGG 0.540164 5:-564513 MsG0580024079.01.T01:CDS
AGCCCCATAATTGGATCCAA+AGG 0.544869 5:+564511 None:intergenic
GGTATCTGGTGTAATCCCTC+AGG 0.550080 5:-564705 MsG0580024079.01.T01:CDS
GAGCAAATTGGATCTCAGAT+TGG 0.550832 5:-564456 MsG0580024079.01.T01:CDS
TGGAGGATAGAGATGGGGAT+AGG 0.554064 5:+564741 None:intergenic
ATTGGCCAGGTACAAGATGT+TGG 0.554788 5:-564438 MsG0580024079.01.T01:CDS
GCTATAGATTTACAGTGAAG+TGG 0.555101 5:+564486 None:intergenic
CAGCAGTTCCTTATGATCCA+TGG 0.556744 5:+564597 None:intergenic
GAGCTCTAAGGGGAAGTCCA+TGG 0.558180 5:-564614 MsG0580024079.01.T01:CDS
ATATCCAATCCCATCAGAGC+AGG 0.561374 5:-564360 MsG0580024079.01.T01:CDS
TGAACTTTGCCGGAGAAGGA+TGG 0.563927 5:-566341 MsG0580024079.01.T01:CDS
CTGGCTGGTCTTACAACCAT+GGG 0.579094 5:-564577 MsG0580024079.01.T01:CDS
AATTGGATCTCAGATTGGCC+AGG 0.579195 5:-564451 MsG0580024079.01.T01:CDS
CTGTAAATCTATAGCTATGG+GGG 0.585985 5:-564478 MsG0580024079.01.T01:CDS
AGGAATCTACATAGGTAATG+AGG 0.587875 5:-564340 MsG0580024079.01.T01:CDS
GCTGGCTGGTCTTACAACCA+TGG 0.594371 5:-564578 MsG0580024079.01.T01:CDS
CCACATTTAAAACAGAACAT+AGG 0.600125 5:+564276 None:intergenic
AACTGTGGAGGATAGAGATG+GGG 0.601332 5:+564736 None:intergenic
TCAAGAATCTCACAATGAAG+AGG 0.602276 5:-564865 MsG0580024079.01.T01:CDS
AGATTCCAACATCTTGTACC+TGG 0.610773 5:+564433 None:intergenic
TGAGGTAGATGGAATTAACT+GGG 0.615787 5:-564322 MsG0580024079.01.T01:CDS
AAATCCAGTCTACTAATGCA+GGG 0.633080 5:-564209 MsG0580024079.01.T01:CDS
GCATTAGTAGACTGGATTTG+GGG 0.636472 5:+564213 None:intergenic
ATCAGAGCAGGAATCTACAT+AGG 0.638479 5:-564348 MsG0580024079.01.T01:CDS
ACTGTAAATCTATAGCTATG+GGG 0.650386 5:-564479 MsG0580024079.01.T01:CDS
CCCATAATTGGATCCAAAGG+GGG 0.650951 5:+564514 None:intergenic
AGATACCTGTTAAGGAACTG+TGG 0.668301 5:+564721 None:intergenic
CCCCATAATTGGATCCAAAG+GGG 0.678833 5:+564513 None:intergenic
GCAGGGAAAACTAATCCAAG+AGG 0.687702 5:-564192 MsG0580024079.01.T01:CDS
TTGATGGTTATGGAATTGAG+GGG 0.691996 5:+564884 None:intergenic
TACCTGTTAAGGAACTGTGG+AGG 0.707328 5:+564724 None:intergenic
GATATCAATAGAGCTCTAAG+GGG 0.715786 5:-564624 MsG0580024079.01.T01:CDS
TCACAATGAAGAGGTCACTG+AGG 0.750511 5:-564856 MsG0580024079.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTAAAATTAATTATTT+TGG - Chr5:564785-564804 MsG0580024079.01.T01:CDS 0.0%
!! AATAAAATAAAAAAGGAAAA+AGG + Chr5:564594-564613 None:intergenic 10.0%
!! ATAAAATAAAAAAGGAAAAA+GGG + Chr5:564593-564612 None:intergenic 10.0%
!!! GTTCAAATAATTTTATTAAT+AGG - Chr5:564883-564902 MsG0580024079.01.T01:CDS 10.0%
!!! TTTTACTGATTTTTTATTAA+TGG - Chr5:564861-564880 MsG0580024079.01.T01:CDS 10.0%
!! AAAAATGTATCAAATTTCTA+TGG - Chr5:565885-565904 MsG0580024079.01.T01:intron 15.0%
!! TTCAAAATTTCAGAAATAAA+AGG - Chr5:565862-565881 MsG0580024079.01.T01:intron 15.0%
!!! TAAAGTAGTTCATAATTTTA+AGG - Chr5:564835-564854 MsG0580024079.01.T01:CDS 15.0%
!!! TCTATTACTATCATTTTTTA+AGG - Chr5:564620-564639 MsG0580024079.01.T01:CDS 15.0%
!!! TTTTTTTCATTACGTTATTA+TGG - Chr5:564517-564536 MsG0580024079.01.T01:CDS 15.0%
!! AGAAAAAGCTAAAATTGTAA+AGG - Chr5:566143-566162 MsG0580024079.01.T01:intron 20.0%
!! ATGAACTACTTTATAACAAT+TGG + Chr5:564828-564847 None:intergenic 20.0%
!!! GTCGTAGTTAAATAATTTTT+TGG - Chr5:564759-564778 MsG0580024079.01.T01:CDS 20.0%
!!! TTACAATTTTAGCTTTTTCT+GGG + Chr5:566143-566162 None:intergenic 20.0%
!!! TTTACAATTTTAGCTTTTTC+TGG + Chr5:566144-566163 None:intergenic 20.0%
! AGAGAGAGAGAATTATTATT+TGG - Chr5:564460-564479 MsG0580024079.01.T01:CDS 25.0%
! AGGTTTAATTGCTCATAAAA+AGG - Chr5:564717-564736 MsG0580024079.01.T01:CDS 25.0%
! AGTGTTGTATGAAAATTGAA+AGG - Chr5:564295-564314 MsG0580024079.01.T01:CDS 25.0%
! CAAAAAATATCAGCTCAAAT+AGG - Chr5:564939-564958 MsG0580024079.01.T01:intron 25.0%
! GTATCAAATTTCTATGGAAA+AGG - Chr5:565891-565910 MsG0580024079.01.T01:intron 25.0%
! GTGTTGTATGAAAATTGAAA+GGG - Chr5:564296-564315 MsG0580024079.01.T01:CDS 25.0%
! TTCATTACGTTATTATGGTA+GGG - Chr5:564522-564541 MsG0580024079.01.T01:CDS 25.0%
! TTGGAAAAATTGAAATCACT+AGG + Chr5:566006-566025 None:intergenic 25.0%
! TTTCATTACGTTATTATGGT+AGG - Chr5:564521-564540 MsG0580024079.01.T01:CDS 25.0%
!! ATATTTTCTGGATTTTTGCA+GGG + Chr5:565471-565490 None:intergenic 25.0%
!! TTTCTTGATAGTAACAGAAT+GGG + Chr5:565717-565736 None:intergenic 25.0%
!!! ACTTTCCATTTATTGACTTA+CGG - Chr5:564679-564698 MsG0580024079.01.T01:CDS 25.0%
!!! CTATGTTCTGTTTTAAATGT+GGG - Chr5:566268-566287 MsG0580024079.01.T01:intron 25.0%
AAAGGGAAATGAATAGGAAA+AGG + Chr5:564576-564595 None:intergenic 30.0%
AAGATATCAATAGAGCTCTA+AGG - Chr5:565917-565936 MsG0580024079.01.T01:intron 30.0%
AAGGAAAAAGGGAAATGAAT+AGG + Chr5:564582-564601 None:intergenic 30.0%
ACTGTAAATCTATAGCTATG+GGG - Chr5:566064-566083 MsG0580024079.01.T01:intron 30.0%
AGGATAAGGGAAAGAATATA+TGG + Chr5:565202-565221 None:intergenic 30.0%
AGTTTGACAAAGTGAAGAAA+GGG - Chr5:564355-564374 MsG0580024079.01.T01:CDS 30.0%
ATAAGGGAAAGAATATATGG+GGG + Chr5:565199-565218 None:intergenic 30.0%
ATCACTGAATTGAGTTATGA+TGG + Chr5:565506-565525 None:intergenic 30.0%
CACTGTAAATCTATAGCTAT+GGG - Chr5:566063-566082 MsG0580024079.01.T01:intron 30.0%
GAATCTTGATTTCCAAGATT+GGG + Chr5:565634-565653 None:intergenic 30.0%
GATAAGGGAAAGAATATATG+GGG + Chr5:565200-565219 None:intergenic 30.0%
GGATAAGGGAAAGAATATAT+GGG + Chr5:565201-565220 None:intergenic 30.0%
TCACTGAATTGAGTTATGAT+GGG + Chr5:565505-565524 None:intergenic 30.0%
TCACTGTAAATCTATAGCTA+TGG - Chr5:566062-566081 MsG0580024079.01.T01:intron 30.0%
TGAATGAAGAAATAAGTGAG+AGG - Chr5:564324-564343 MsG0580024079.01.T01:CDS 30.0%
! AGATATCAATAGAGCTCTAA+GGG - Chr5:565918-565937 MsG0580024079.01.T01:intron 30.0%
! CAATTTTCATACAACACTCA+TGG + Chr5:564293-564312 None:intergenic 30.0%
! CCACATTTAAAACAGAACAT+AGG + Chr5:566270-566289 None:intergenic 30.0%
! GATATTTTCTGGATTTTTGC+AGG + Chr5:565472-565491 None:intergenic 30.0%
! GTTTCTTGATAGTAACAGAA+TGG + Chr5:565718-565737 None:intergenic 30.0%
! TTTGGTTTCTTGCTGTATAA+TGG - Chr5:564478-564497 MsG0580024079.01.T01:CDS 30.0%
!! ATGATGGTGATTTTATTGCA+AGG - Chr5:564233-564252 MsG0580024079.01.T01:CDS 30.0%
!! CCTATGTTCTGTTTTAAATG+TGG - Chr5:566267-566286 MsG0580024079.01.T01:intron 30.0%
!! CTTTCCATTTATTGACTTAC+GGG - Chr5:564680-564699 MsG0580024079.01.T01:CDS 30.0%
!! TGTTTTAAATGTGGGCTAAT+AGG - Chr5:566276-566295 MsG0580024079.01.T01:intron 30.0%
!!! AGATGACTCCTTTGTTTTTA+GGG + Chr5:565396-565415 None:intergenic 30.0%
!!! TCTGAAATTTTGAAACCTGA+GGG + Chr5:565856-565875 None:intergenic 30.0%
!!! TTCTGAAATTTTGAAACCTG+AGG + Chr5:565857-565876 None:intergenic 30.0%
AAATCCAGTCTACTAATGCA+GGG - Chr5:566334-566353 MsG0580024079.01.T01:CDS 35.0%
AAGATTAGTGAAAGGGATTG+TGG + Chr5:565230-565249 None:intergenic 35.0%
AAGGTAGCAAGATTAGTGAA+AGG + Chr5:565238-565257 None:intergenic 35.0%
AATATATGGGGGAGAAAAAG+AGG + Chr5:565188-565207 None:intergenic 35.0%
AGGAATCTACATAGGTAATG+AGG - Chr5:566203-566222 MsG0580024079.01.T01:intron 35.0%
AGGTAGCAAGATTAGTGAAA+GGG + Chr5:565237-565256 None:intergenic 35.0%
ATATCTAAGCGTATCATTGC+TGG + Chr5:565590-565609 None:intergenic 35.0%
ATGAGGTAGATGGAATTAAC+TGG - Chr5:566220-566239 MsG0580024079.01.T01:intron 35.0%
ATGTCAGACCCTAAAAACAA+AGG - Chr5:565385-565404 MsG0580024079.01.T01:intron 35.0%
ATTACACCAGATACCTGTTA+AGG + Chr5:565833-565852 None:intergenic 35.0%
CACTGAATTGAGTTATGATG+GGG + Chr5:565504-565523 None:intergenic 35.0%
CTCAAAAACCAACGAAATAC+AGG + Chr5:564432-564451 None:intergenic 35.0%
CTGTAAATCTATAGCTATGG+GGG - Chr5:566065-566084 MsG0580024079.01.T01:intron 35.0%
CTTCATTGTGAGATTCTTGA+TGG + Chr5:565678-565697 None:intergenic 35.0%
GAGATAAAGAAAGAAACCAG+AGG + Chr5:565304-565323 None:intergenic 35.0%
GAGTTTGACAAAGTGAAGAA+AGG - Chr5:564354-564373 MsG0580024079.01.T01:CDS 35.0%
GCTATAGATTTACAGTGAAG+TGG + Chr5:566060-566079 None:intergenic 35.0%
GGAATCTTGATTTCCAAGAT+TGG + Chr5:565635-565654 None:intergenic 35.0%
GTTTGACAAAGTGAAGAAAG+GGG - Chr5:564356-564375 MsG0580024079.01.T01:CDS 35.0%
TACATAGGTAATGAGGTAGA+TGG - Chr5:566210-566229 MsG0580024079.01.T01:intron 35.0%
TATCATCGCATAAACGATGA+AGG + Chr5:565080-565099 None:intergenic 35.0%
TCAAGAATCTCACAATGAAG+AGG - Chr5:565678-565697 MsG0580024079.01.T01:intron 35.0%
TCATTCGTACGGATCTTAAT+CGG + Chr5:564262-564281 None:intergenic 35.0%
TGAGGTAGATGGAATTAACT+GGG - Chr5:566221-566240 MsG0580024079.01.T01:intron 35.0%
TGCATTAGTAGACTGGATTT+GGG + Chr5:566334-566353 None:intergenic 35.0%
TTTGACAAAGTGAAGAAAGG+GGG - Chr5:564357-564376 MsG0580024079.01.T01:CDS 35.0%
! AAAGAAACCAGAGGTGTAAA+AGG + Chr5:565295-565314 None:intergenic 35.0%
! CTTGATGGTTATGGAATTGA+GGG + Chr5:565663-565682 None:intergenic 35.0%
! TCTTGATGGTTATGGAATTG+AGG + Chr5:565664-565683 None:intergenic 35.0%
! TGTGAGATTCTTGATGGTTA+TGG + Chr5:565672-565691 None:intergenic 35.0%
! TGTTTGGTGAAAGTTGTTTG+AGG + Chr5:565425-565444 None:intergenic 35.0%
! TTGATGGTTATGGAATTGAG+GGG + Chr5:565662-565681 None:intergenic 35.0%
!! AGAATTGAAAGAGCATCACA+AGG + Chr5:565123-565142 None:intergenic 35.0%
!! GATATCAATAGAGCTCTAAG+GGG - Chr5:565919-565938 MsG0580024079.01.T01:intron 35.0%
!!! AAGGATGGTTTTGATGATGA+TGG - Chr5:564217-564236 MsG0580024079.01.T01:CDS 35.0%
!!! GAGATGACTCCTTTGTTTTT+AGG + Chr5:565397-565416 None:intergenic 35.0%
!!! GTGGTTGGTTTTTGTATGTT+GGG - Chr5:564379-564398 MsG0580024079.01.T01:CDS 35.0%
!!! TGGTTGGTTTTTGTATGTTG+GGG - Chr5:564380-564399 MsG0580024079.01.T01:CDS 35.0%
!!! TTTGTTTTTAGGGTCTGACA+TGG + Chr5:565386-565405 None:intergenic 35.0%
AAAGGCTAAGGGAAAGAGAT+TGG + Chr5:565277-565296 None:intergenic 40.0%
ACAGTTCCTTAACAGGTATC+TGG - Chr5:565824-565843 MsG0580024079.01.T01:intron 40.0%
AGATACCTGTTAAGGAACTG+TGG + Chr5:565825-565844 None:intergenic 40.0%
AGATTCCAACATCTTGTACC+TGG + Chr5:566113-566132 None:intergenic 40.0%
ATCACAAGGTGGAGAAACTA+CGG + Chr5:565109-565128 None:intergenic 40.0%
ATCAGAGCAGGAATCTACAT+AGG - Chr5:566195-566214 MsG0580024079.01.T01:intron 40.0%
ATGTAGATTCCTGCTCTGAT+GGG + Chr5:566195-566214 None:intergenic 40.0%
CAAATCCAGTCTACTAATGC+AGG - Chr5:566333-566352 MsG0580024079.01.T01:CDS 40.0%
CATAACCACCAAGAACACAT+TGG - Chr5:565562-565581 MsG0580024079.01.T01:intron 40.0%
CGATCCCGTAAGTCAATAAA+TGG + Chr5:564687-564706 None:intergenic 40.0%
CTGCATTAGTAGACTGGATT+TGG + Chr5:566335-566354 None:intergenic 40.0%
GAGCAAATTGGATCTCAGAT+TGG - Chr5:566087-566106 MsG0580024079.01.T01:intron 40.0%
GATTGTGGAAGAGAGGATAA+GGG + Chr5:565215-565234 None:intergenic 40.0%
GCATTAGTAGACTGGATTTG+GGG + Chr5:566333-566352 None:intergenic 40.0%
GCGATAATCGCAAAAAAGAG+AGG + Chr5:564996-565015 None:intergenic 40.0%
TAAGGGAAAGAGATTGGTGT+GGG + Chr5:565271-565290 None:intergenic 40.0%
TATGTAGATTCCTGCTCTGA+TGG + Chr5:566196-566215 None:intergenic 40.0%
TCATCAAAACCATCCTTCTC+CGG + Chr5:564214-564233 None:intergenic 40.0%
TGAAGGAAAACTCAGCATAG+CGG + Chr5:565063-565082 None:intergenic 40.0%
TGGAGATCAAGAAAGGAAAG+AGG + Chr5:565161-565180 None:intergenic 40.0%
TGTATGTTGGGGATTGAGTA+TGG - Chr5:564391-564410 MsG0580024079.01.T01:CDS 40.0%
! AAGGAAAGAGGCACATCATA+AGG + Chr5:565149-565168 None:intergenic 40.0%
! ATTTTTCCAAGATCCCCCTT+TGG - Chr5:566016-566035 MsG0580024079.01.T01:intron 40.0%
! GGCTAATGGCTGATATTTTC+TGG + Chr5:565483-565502 None:intergenic 40.0%
! TTGAGTTATGATGGGGCTAA+TGG + Chr5:565497-565516 None:intergenic 40.0%
! TTTTCCCTGCATTAGTAGAC+TGG + Chr5:566341-566360 None:intergenic 40.0%
! TTTTCTGGATTTTTGCAGGG+CGG + Chr5:565468-565487 None:intergenic 40.0%
!! AAGTTGGTATGAGGATGGAA+GGG + Chr5:565361-565380 None:intergenic 40.0%
!! ATTGAAAGAGCATCACAAGG+TGG + Chr5:565120-565139 None:intergenic 40.0%
!! TAATGGGGCTAGTTTCTGTT+TGG + Chr5:565441-565460 None:intergenic 40.0%
!! TACGGGATCGATTTTCTACT+AGG - Chr5:564697-564716 MsG0580024079.01.T01:CDS 40.0%
!!! GGTGGTTATGCTGATTTTGT+TGG + Chr5:565552-565571 None:intergenic 40.0%
!!! GGTGGTTGGTTTTTGTATGT+TGG - Chr5:564378-564397 MsG0580024079.01.T01:CDS 40.0%
AACTGTGGAGGATAGAGATG+GGG + Chr5:565810-565829 None:intergenic 45.0%
AAGATCCGTACGAATGAGCA+CGG - Chr5:564265-564284 MsG0580024079.01.T01:CDS 45.0%
AGCCCCATAATTGGATCCAA+AGG + Chr5:566035-566054 None:intergenic 45.0%
AGGCAGATGGAGATCAAGAA+AGG + Chr5:565168-565187 None:intergenic 45.0%
ATAGCTATGGGGGAGCAAAT+TGG - Chr5:566075-566094 MsG0580024079.01.T01:intron 45.0%
ATATCCAATCCCATCAGAGC+AGG - Chr5:566183-566202 MsG0580024079.01.T01:intron 45.0%
ATCCTCCACAGTTCCTTAAC+AGG - Chr5:565817-565836 MsG0580024079.01.T01:intron 45.0%
ATTGGCCAGGTACAAGATGT+TGG - Chr5:566105-566124 MsG0580024079.01.T01:intron 45.0%
CAGCAGTTCCTTATGATCCA+TGG + Chr5:565949-565968 None:intergenic 45.0%
CATGGATCATAAGGAACTGC+TGG - Chr5:565947-565966 MsG0580024079.01.T01:intron 45.0%
CCCATAATTGGATCCAAAGG+GGG + Chr5:566032-566051 None:intergenic 45.0%
CCCCATAATTGGATCCAAAG+GGG + Chr5:566033-566052 None:intergenic 45.0%
CCCCCTTTGGATCCAATTAT+GGG - Chr5:566029-566048 MsG0580024079.01.T01:intron 45.0%
CCCCTTTGGATCCAATTATG+GGG - Chr5:566030-566049 MsG0580024079.01.T01:intron 45.0%
CTAAGGGAAAGAGATTGGTG+TGG + Chr5:565272-565291 None:intergenic 45.0%
GAACTGTGGAGGATAGAGAT+GGG + Chr5:565811-565830 None:intergenic 45.0%
GCAGGGAAAACTAATCCAAG+AGG - Chr5:566351-566370 MsG0580024079.01.T01:CDS 45.0%
GCCCCATAATTGGATCCAAA+GGG + Chr5:566034-566053 None:intergenic 45.0%
GCTTGAAGGATGAACTTTGC+CGG - Chr5:564192-564211 MsG0580024079.01.T01:CDS 45.0%
GGATTGTGGAAGAGAGGATA+AGG + Chr5:565216-565235 None:intergenic 45.0%
GGATTTACTTGTCTGTCCCA+TGG + Chr5:565985-566004 None:intergenic 45.0%
GGGATTGAGTATGGAAACTG+AGG - Chr5:564400-564419 MsG0580024079.01.T01:CDS 45.0%
GGTTATGGAATTGAGGGGTT+GGG + Chr5:565657-565676 None:intergenic 45.0%
GTTATGGAATTGAGGGGTTG+GGG + Chr5:565656-565675 None:intergenic 45.0%
TACCTGTTAAGGAACTGTGG+AGG + Chr5:565822-565841 None:intergenic 45.0%
TCACAATGAAGAGGTCACTG+AGG - Chr5:565687-565706 MsG0580024079.01.T01:intron 45.0%
TCCCCCTTTGGATCCAATTA+TGG - Chr5:566028-566047 MsG0580024079.01.T01:intron 45.0%
TGAAAGGGATTGTGGAAGAG+AGG + Chr5:565222-565241 None:intergenic 45.0%
TGGTTATGGAATTGAGGGGT+TGG + Chr5:565658-565677 None:intergenic 45.0%
TGGTTCCAATGTGTTCTTGG+TGG + Chr5:565570-565589 None:intergenic 45.0%
TGTTGCCAATGATGCTGTTC+TGG + Chr5:565768-565787 None:intergenic 45.0%
TTCCAAGATTGGGCTGTTGT+GGG + Chr5:565624-565643 None:intergenic 45.0%
TTTCCAAGATTGGGCTGTTG+TGG + Chr5:565625-565644 None:intergenic 45.0%
! AAACTCAGCATAGCGGAAAG+CGG + Chr5:565056-565075 None:intergenic 45.0%
! AATTGGATCTCAGATTGGCC+AGG - Chr5:566092-566111 MsG0580024079.01.T01:intron 45.0%
! ACCAGAGGTGTAAAAGGCTA+AGG + Chr5:565289-565308 None:intergenic 45.0%
! CCAGAGGTGTAAAAGGCTAA+GGG + Chr5:565288-565307 None:intergenic 45.0%
! TGGTGTGGGAACTTTTCTCA+AGG + Chr5:565257-565276 None:intergenic 45.0%
!! GAAGTTGGTATGAGGATGGA+AGG + Chr5:565362-565381 None:intergenic 45.0%
!! GACATGGAGAAGTTGGTATG+AGG + Chr5:565370-565389 None:intergenic 45.0%
!! TGCTGGTTCCAATGTGTTCT+TGG + Chr5:565573-565592 None:intergenic 45.0%
!! TGGAGAAGTTGGTATGAGGA+TGG + Chr5:565366-565385 None:intergenic 45.0%
!! AAAAAAGAATAAAATAAAAA+AGG + Chr5:564601-564620 None:intergenic 5.0%
AAGTGAAGAAAGGGGGTGGT+TGG - Chr5:564364-564383 MsG0580024079.01.T01:CDS 50.0%
ACCCAACAAGGGGGAAAGAA+CGG + Chr5:565337-565356 None:intergenic 50.0%
AGACTGGATTTGGGGAGAAG+TGG + Chr5:566325-566344 None:intergenic 50.0%
AGGATGAACTTTGCCGGAGA+AGG - Chr5:564198-564217 MsG0580024079.01.T01:CDS 50.0%
AGGGTCTGACATGGAGAAGT+TGG + Chr5:565377-565396 None:intergenic 50.0%
ATCCCACAACAGCCCAATCT+TGG - Chr5:565619-565638 MsG0580024079.01.T01:intron 50.0%
ATGGAAGGGAACCCAACAAG+GGG + Chr5:565347-565366 None:intergenic 50.0%
GAAGTGGCAAGCCCCATAAT+TGG + Chr5:566044-566063 None:intergenic 50.0%
GACAAAGTGAAGAAAGGGGG+TGG - Chr5:564360-564379 MsG0580024079.01.T01:CDS 50.0%
GATCATAAGGAACTGCTGGC+TGG - Chr5:565951-565970 MsG0580024079.01.T01:intron 50.0%
GATGGAAGGGAACCCAACAA+GGG + Chr5:565348-565367 None:intergenic 50.0%
GATTCCTGCTCTGATGGGAT+TGG + Chr5:566190-566209 None:intergenic 50.0%
GGAACTGTGGAGGATAGAGA+TGG + Chr5:565812-565831 None:intergenic 50.0%
GGGGAAGTCCATGGATCATA+AGG - Chr5:565938-565957 MsG0580024079.01.T01:intron 50.0%
GGTATCTGGTGTAATCCCTC+AGG - Chr5:565838-565857 MsG0580024079.01.T01:intron 50.0%
GGTGACAGCCTGTATTTCGT+TGG - Chr5:564421-564440 MsG0580024079.01.T01:CDS 50.0%
TCCGTTCTTTCCCCCTTGTT+GGG - Chr5:565333-565352 MsG0580024079.01.T01:intron 50.0%
TCCTGCCAGAACAGCATCAT+TGG - Chr5:565760-565779 MsG0580024079.01.T01:intron 50.0%
TGAACTTTGCCGGAGAAGGA+TGG - Chr5:564202-564221 MsG0580024079.01.T01:CDS 50.0%
TGGAGGATAGAGATGGGGAT+AGG + Chr5:565805-565824 None:intergenic 50.0%
TGGATCCGTGCTCATTCGTA+CGG + Chr5:564273-564292 None:intergenic 50.0%
TTGGATCCAAAGGGGGATCT+TGG + Chr5:566025-566044 None:intergenic 50.0%
!! CCCTTAGCCTTTTACACCTC+TGG - Chr5:565285-565304 MsG0580024079.01.T01:intron 50.0%
!! CTGGCTGGTCTTACAACCAT+GGG - Chr5:565966-565985 MsG0580024079.01.T01:intron 50.0%
CAACAAGGGGGAAAGAACGG+AGG + Chr5:565334-565353 None:intergenic 55.0%
CTCCGTTCTTTCCCCCTTGT+TGG - Chr5:565332-565351 MsG0580024079.01.T01:intron 55.0%
GCCAATGATGCTGTTCTGGC+AGG + Chr5:565764-565783 None:intergenic 55.0%
GGATGGAAGGGAACCCAACA+AGG + Chr5:565349-565368 None:intergenic 55.0%
GGGGGAGAAAAAGAGGCAGA+TGG + Chr5:565181-565200 None:intergenic 55.0%
TGGAAGGGAACCCAACAAGG+GGG + Chr5:565346-565365 None:intergenic 55.0%
TTTGCAGGGCGGTGGGTAAT+GGG + Chr5:565457-565476 None:intergenic 55.0%
! CTGGATTTTTGCAGGGCGGT+GGG + Chr5:565464-565483 None:intergenic 55.0%
! TCTGGATTTTTGCAGGGCGG+TGG + Chr5:565465-565484 None:intergenic 55.0%
! TTTTGCAGGGCGGTGGGTAA+TGG + Chr5:565458-565477 None:intergenic 55.0%
!! GAGCTCTAAGGGGAAGTCCA+TGG - Chr5:565929-565948 MsG0580024079.01.T01:intron 55.0%
!! GCTGGCTGGTCTTACAACCA+TGG - Chr5:565965-565984 MsG0580024079.01.T01:intron 55.0%
AGGGGGAAAGAACGGAGGAG+TGG + Chr5:565329-565348 None:intergenic 60.0%
TTGCAGGGCGGTGGGTAATG+GGG + Chr5:565456-565475 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 564185 566380 564185 ID=MsG0580024079.01;Name=MsG0580024079.01
Chr5 mRNA 564185 566380 564185 ID=MsG0580024079.01.T01;Parent=MsG0580024079.01;Name=MsG0580024079.01.T01;_AED=0.46;_eAED=0.57;_QI=0|0|0|1|1|1|2|0|282
Chr5 exon 566294 566380 566294 ID=MsG0580024079.01.T01:exon:212;Parent=MsG0580024079.01.T01
Chr5 exon 564185 564946 564185 ID=MsG0580024079.01.T01:exon:211;Parent=MsG0580024079.01.T01
Chr5 CDS 566294 566380 566294 ID=MsG0580024079.01.T01:cds;Parent=MsG0580024079.01.T01
Chr5 CDS 564185 564946 564185 ID=MsG0580024079.01.T01:cds;Parent=MsG0580024079.01.T01
Gene Sequence

>MsG0580024079.01.T01

ATGTGGAGCTTGAAGGATGAACTTTGCCGGAGAAGGATGGTTTTGATGATGATGGTGATTTTATTGCAAGGTTTCCGATTAAGATCCATCCCACAACAGCCCAATCTTGGAAATCAAGATTCCCCAACCCCTCAATTCCATAACCATCAAGAATCTCACAATGAAGAGGTCACTGAGGAAGACCCATTCTGTTACTATCAAGAAACACACGTTCAAGATAGTGTTAGCTCCTGCCAGAACAGCATCATTGGCAACATCTTAGCAGACAAACCTATCCCCATCTCTATCCTCCACAGTTCCTTAACAGGTATCTGGTGTAATCCCTCAGGTTTCAAAATTTCAGAAATAAAAGGAAAAATGTATCAAATTTCTATGGAAAAGGAGCAAGATATCAATAGAGCTCTAAGGGGAAGTCCATGGATCATAAGGAACTGCTGGCTGGTCTTACAACCATGGGACAGACAAGTAAATCCTAGTGATTTCAATTTTTCCAAGATCCCCCTTTGGATCCAATTATGGGGCTTGCCACTTCACTGTAAATCTATAGCTATGGGGGAGCAAATTGGATCTCAGATTGGCCAGGTACAAGATGTTGGAATCTATGAGAACCCAGAAAAAGCTAAAATTGTAAAGGTAAAAATTCTCTTTGATATATCCAATCCCATCAGAGCAGGAATCTACATAGGTAATGAGGTAGATGGAATTAACTGGGTTGACTTTCGCTTTGAGAATTTGCCTATGTTCTGTTTTAAATGTGGGCTAATAGGACACAATGAAGAAAATTGCTATTCCACTTCTCCCCAAATCCAGTCTACTAATGCAGGGAAAACTAATCCAAGAGGAGCCTGA
Protein sequence

>MsG0580024079.01.T01

MWSLKDELCRRRMVLMMMVILLQGFRLRSIPQQPNLGNQDSPTPQFHNHQESHNEEVTEEDPFCYYQETHVQDSVSSCQNSIIGNILADKPIPISILHSSLTGIWCNPSGFKISEIKGKMYQISMEKEQDINRALRGSPWIIRNCWLVLQPWDRQVNPSDFNFSKIPLWIQLWGLPLHCKSIAMGEQIGSQIGQVQDVGIYENPEKAKIVKVKILFDISNPIRAGIYIGNEVDGINWVDFRFENLPMFCFKCGLIGHNEENCYSTSPQIQSTNAGKTNPRGA*