Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024080.01.T01 | RHN53256.1 | 99.522 | 209 | 1 | 0 | 1 | 209 | 272 | 480 | 1.01E-148 | 432 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024080.01.T01 | A0A396HJ24 | 99.522 | 209 | 1 | 0 | 1 | 209 | 272 | 480 | 4.85e-149 | 432 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005199.01 | MsG0580024080.01 | 0.868912 | 4.588090e-66 | 1.688598e-62 |
| MsG0280006293.01 | MsG0580024080.01 | 0.835296 | 1.822737e-56 | 2.219770e-53 |
| MsG0480021652.01 | MsG0580024080.01 | 0.805580 | 1.246315e-49 | 6.617751e-47 |
| MsG0480021859.01 | MsG0580024080.01 | 0.833429 | 5.359814e-56 | 6.170899e-53 |
| MsG0580024079.01 | MsG0580024080.01 | 0.816126 | 6.492196e-52 | 4.561220e-49 |
| MsG0580024080.01 | MsG0780039440.01 | 0.846291 | 2.382169e-59 | 4.080425e-56 |
| MsG0580024080.01 | MsG0880047123.01 | 0.808641 | 2.803443e-50 | 1.611352e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024080.01.T01 | MTR_5g005420 | 99.522 | 209 | 1 | 0 | 1 | 209 | 209 | 417 | 2.28e-152 | 429 |
| MsG0580024080.01.T01 | MTR_3g110630 | 56.332 | 229 | 64 | 4 | 2 | 209 | 235 | 448 | 1.16e-68 | 217 |
| MsG0580024080.01.T01 | MTR_6g090060 | 52.885 | 208 | 71 | 5 | 2 | 207 | 232 | 414 | 6.10e-64 | 204 |
| MsG0580024080.01.T01 | MTR_8g009770 | 45.662 | 219 | 90 | 5 | 2 | 208 | 229 | 430 | 2.10e-49 | 166 |
| MsG0580024080.01.T01 | MTR_8g102270 | 48.039 | 204 | 65 | 6 | 13 | 207 | 183 | 354 | 3.38e-48 | 161 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580024080.01.T01 | AT3G19680 | 62.882 | 229 | 59 | 5 | 2 | 209 | 261 | 484 | 2.63e-78 | 243 |
| MsG0580024080.01.T01 | AT1G50040 | 63.426 | 216 | 70 | 4 | 2 | 209 | 239 | 453 | 5.98e-68 | 215 |
| MsG0580024080.01.T01 | AT4G29310 | 52.381 | 210 | 71 | 4 | 2 | 207 | 231 | 415 | 3.04e-63 | 202 |
| MsG0580024080.01.T01 | AT1G10020 | 53.153 | 222 | 87 | 5 | 2 | 207 | 235 | 455 | 6.61e-62 | 199 |
| MsG0580024080.01.T01 | AT5G17640 | 48.293 | 205 | 82 | 5 | 2 | 197 | 228 | 417 | 6.75e-46 | 157 |
| MsG0580024080.01.T01 | AT4G29310 | 67.901 | 81 | 24 | 1 | 2 | 80 | 231 | 311 | 4.85e-33 | 121 |
Find 66 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAACGGAAGCTGGGATTT+AGG | 0.285254 | 5:+567236 | MsG0580024080.01.T01:CDS |
| TCCAACGGAGTTTGAGTAAC+CGG | 0.343106 | 5:-567090 | None:intergenic |
| TCAAGACCCGGGTCTGGTTC+CGG | 0.376107 | 5:+567272 | MsG0580024080.01.T01:CDS |
| CTGCAACCGGCGAGTGTGAC+TGG | 0.377352 | 5:-567146 | None:intergenic |
| ACCGGTTGCGTGTGCTTCAA+TGG | 0.380208 | 5:+566938 | MsG0580024080.01.T01:CDS |
| AACCCGATGGTGATGGTACT+TGG | 0.396043 | 5:+567033 | MsG0580024080.01.T01:CDS |
| ATACCGGTTCGACGTTCTTC+CGG | 0.400080 | 5:+567112 | MsG0580024080.01.T01:CDS |
| CTTCCGTTTGGAGTGTTAAC+GGG | 0.405734 | 5:-567224 | None:intergenic |
| AGCTGCTATGGATTTGAGTA+TGG | 0.413553 | 5:+567448 | MsG0580024080.01.T01:CDS |
| TCCTTCACCTGGTTCACAAC+AGG | 0.413673 | 5:+566974 | MsG0580024080.01.T01:CDS |
| TGGGATTTAGGATCCGGTTC+AGG | 0.417320 | 5:+567248 | MsG0580024080.01.T01:CDS |
| TGTTGCTCTTGCAGCTGCTA+TGG | 0.432619 | 5:+567436 | MsG0580024080.01.T01:CDS |
| AGCACACGCAACCGGTGAAC+CGG | 0.433904 | 5:-566931 | None:intergenic |
| ACCATTGAAGCACACGCAAC+CGG | 0.440718 | 5:-566939 | None:intergenic |
| CCTAAATCCCAGCTTCCGTT+TGG | 0.449770 | 5:-567236 | None:intergenic |
| GCTTCCGTTTGGAGTGTTAA+CGG | 0.458009 | 5:-567225 | None:intergenic |
| GGAAGCTGGGATTTAGGATC+CGG | 0.461778 | 5:+567242 | MsG0580024080.01.T01:CDS |
| ACACCGTTCGTTCCTTCACC+TGG | 0.465337 | 5:+566963 | MsG0580024080.01.T01:CDS |
| TTAACACTCCAAACGGAAGC+TGG | 0.465898 | 5:+567228 | MsG0580024080.01.T01:CDS |
| TTCACACCAACTTCCACCTC+CGG | 0.469096 | 5:-567380 | None:intergenic |
| TTTACAATTGATGTAACATC+AGG | 0.469500 | 5:+567194 | MsG0580024080.01.T01:CDS |
| CTGTGAACTGATACTGCAAC+CGG | 0.471720 | 5:-567159 | None:intergenic |
| CCCGGGTCTTGATCCTGAAC+CGG | 0.472126 | 5:-567261 | None:intergenic |
| ATCTGAACCGGAACCAGACC+CGG | 0.472927 | 5:-567279 | None:intergenic |
| TTTCCATCAATGGATAGAAA+AGG | 0.487017 | 5:+566882 | None:intergenic |
| ATTACGATACACGATTTATC+CGG | 0.490765 | 5:+566912 | MsG0580024080.01.T01:CDS |
| CATGGGGTCGACTCGAAGCT+TGG | 0.495685 | 5:+567060 | MsG0580024080.01.T01:CDS |
| GTGATGTCGGCGAGATTTGA+CGG | 0.496053 | 5:+567338 | MsG0580024080.01.T01:CDS |
| CTCGAAGCTTGGCGTGAACC+CGG | 0.500118 | 5:+567071 | MsG0580024080.01.T01:CDS |
| GTGACTGGATCAGCGGTGGC+CGG | 0.500258 | 5:-567131 | None:intergenic |
| TTCCAAGTACCATCACCATC+GGG | 0.502674 | 5:-567035 | None:intergenic |
| ACTCAAACTCCGTTGGATAC+CGG | 0.505974 | 5:+567096 | MsG0580024080.01.T01:CDS |
| AAAGTGTAGTAAACCGGAGG+TGG | 0.509798 | 5:+567367 | MsG0580024080.01.T01:CDS |
| TTGATAATTAACCAAGCACC+TGG | 0.518437 | 5:-567011 | None:intergenic |
| TCAGGATCAAGACCCGGGTC+TGG | 0.518778 | 5:+567266 | MsG0580024080.01.T01:CDS |
| ACACGATTTATCCGGTTCAC+CGG | 0.530542 | 5:+566920 | MsG0580024080.01.T01:CDS |
| TGGCCGGAAGAACGTCGAAC+CGG | 0.534116 | 5:-567115 | None:intergenic |
| CCCGGTTACTCAAACTCCGT+TGG | 0.543060 | 5:+567089 | MsG0580024080.01.T01:CDS |
| TGCAGTATCAGTTCACAGAA+TGG | 0.545387 | 5:+567164 | MsG0580024080.01.T01:CDS |
| GTAAATCAAACCCAGGTGCT+TGG | 0.549731 | 5:+567000 | MsG0580024080.01.T01:CDS |
| AGTAAACCGGAGGTGGAAGT+TGG | 0.559317 | 5:+567374 | MsG0580024080.01.T01:CDS |
| ACTCCCGTTAACACTCCAAA+CGG | 0.562154 | 5:+567221 | MsG0580024080.01.T01:CDS |
| CCAACGGAGTTTGAGTAACC+GGG | 0.565981 | 5:-567089 | None:intergenic |
| TTTCCAAGTACCATCACCAT+CGG | 0.566391 | 5:-567036 | None:intergenic |
| TTACTGACCTGTTGTGAACC+AGG | 0.566852 | 5:-566981 | None:intergenic |
| TCTGAACCGGAACCAGACCC+GGG | 0.569477 | 5:-567278 | None:intergenic |
| TCCGGTTCAGGATCAAGACC+CGG | 0.572090 | 5:+567260 | MsG0580024080.01.T01:CDS |
| TAACACTCCAAACGGAAGCT+GGG | 0.572899 | 5:+567229 | MsG0580024080.01.T01:CDS |
| TGATGGTACTTGGAAACCAT+GGG | 0.577535 | 5:+567043 | MsG0580024080.01.T01:CDS |
| CATCAATGGATAGAAAAGGT+TGG | 0.582790 | 5:+566886 | None:intergenic |
| CCGGTTCAGGATCAAGACCC+GGG | 0.584337 | 5:+567261 | MsG0580024080.01.T01:CDS |
| GTGATGGTACTTGGAAACCA+TGG | 0.592053 | 5:+567042 | MsG0580024080.01.T01:CDS |
| ATTATCAAACCCGATGGTGA+TGG | 0.600303 | 5:+567026 | MsG0580024080.01.T01:CDS |
| TGGTTAATTATCAAACCCGA+TGG | 0.611258 | 5:+567020 | MsG0580024080.01.T01:CDS |
| CAAGCTTCGAGTCGACCCCA+TGG | 0.631720 | 5:-567059 | None:intergenic |
| GGCGAGTGTGACTGGATCAG+CGG | 0.640369 | 5:-567138 | None:intergenic |
| GAGTGTGACTGGATCAGCGG+TGG | 0.643291 | 5:-567135 | None:intergenic |
| AACGTCGAACCGGTATCCAA+CGG | 0.651013 | 5:-567105 | None:intergenic |
| ACCTGTTGTGAACCAGGTGA+AGG | 0.654247 | 5:-566975 | None:intergenic |
| GCTGATCCAGTCACACTCGC+CGG | 0.659941 | 5:+567140 | MsG0580024080.01.T01:CDS |
| TGATAATTAACCAAGCACCT+GGG | 0.663225 | 5:-567010 | None:intergenic |
| GAACCAGGTGAAGGAACGAA+CGG | 0.681706 | 5:-566966 | None:intergenic |
| AGTATCAGTTCACAGAATGG+TGG | 0.683678 | 5:+567167 | MsG0580024080.01.T01:CDS |
| TGTGAAGCACGTGACATGCA+CGG | 0.711455 | 5:+567397 | MsG0580024080.01.T01:CDS |
| CAGGTCAGTAAATCAAACCC+AGG | 0.712291 | 5:+566993 | MsG0580024080.01.T01:CDS |
| GATGGTACTTGGAAACCATG+GGG | 0.759321 | 5:+567044 | MsG0580024080.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTACAATTGATGTAACATC+AGG | + | Chr5:567194-567213 | MsG0580024080.01.T01:CDS | 25.0% |
| !! | TGAAAAAAAGTGTAGTAAAC+CGG | + | Chr5:567361-567380 | MsG0580024080.01.T01:CDS | 25.0% |
| ATTACGATACACGATTTATC+CGG | + | Chr5:566912-566931 | MsG0580024080.01.T01:CDS | 30.0% | |
| ! | GAGAATCAGTTTTTGTATAG+CGG | + | Chr5:567311-567330 | MsG0580024080.01.T01:CDS | 30.0% |
| CTCCAATGAAAAATCTGAAC+CGG | - | Chr5:567294-567313 | None:intergenic | 35.0% | |
| TGATAATTAACCAAGCACCT+GGG | - | Chr5:567013-567032 | None:intergenic | 35.0% | |
| TGGTTAATTATCAAACCCGA+TGG | + | Chr5:567020-567039 | MsG0580024080.01.T01:CDS | 35.0% | |
| ! | AAAAAAGTGTAGTAAACCGG+AGG | + | Chr5:567364-567383 | MsG0580024080.01.T01:CDS | 35.0% |
| ! | TTCCGGTTCAGATTTTTCAT+TGG | + | Chr5:567289-567308 | MsG0580024080.01.T01:CDS | 35.0% |
| ! | TTGATAATTAACCAAGCACC+TGG | - | Chr5:567014-567033 | None:intergenic | 35.0% |
| AGCTGCTATGGATTTGAGTA+TGG | + | Chr5:567448-567467 | MsG0580024080.01.T01:CDS | 40.0% | |
| AGTATCAGTTCACAGAATGG+TGG | + | Chr5:567167-567186 | MsG0580024080.01.T01:CDS | 40.0% | |
| ATTATCAAACCCGATGGTGA+TGG | + | Chr5:567026-567045 | MsG0580024080.01.T01:CDS | 40.0% | |
| TGATGGTACTTGGAAACCAT+GGG | + | Chr5:567043-567062 | MsG0580024080.01.T01:CDS | 40.0% | |
| TGCAGTATCAGTTCACAGAA+TGG | + | Chr5:567164-567183 | MsG0580024080.01.T01:CDS | 40.0% | |
| TTTCCAAGTACCATCACCAT+CGG | - | Chr5:567039-567058 | None:intergenic | 40.0% | |
| !! | GTATAGCGGTTTTGTGATGT+CGG | + | Chr5:567325-567344 | MsG0580024080.01.T01:CDS | 40.0% |
| AAAGTGTAGTAAACCGGAGG+TGG | + | Chr5:567367-567386 | MsG0580024080.01.T01:CDS | 45.0% | |
| ACACGATTTATCCGGTTCAC+CGG | + | Chr5:566920-566939 | MsG0580024080.01.T01:CDS | 45.0% | |
| ACTCAAACTCCGTTGGATAC+CGG | + | Chr5:567096-567115 | MsG0580024080.01.T01:CDS | 45.0% | |
| ACTCCCGTTAACACTCCAAA+CGG | + | Chr5:567221-567240 | MsG0580024080.01.T01:CDS | 45.0% | |
| CAGGTCAGTAAATCAAACCC+AGG | + | Chr5:566993-567012 | MsG0580024080.01.T01:CDS | 45.0% | |
| CTGTGAACTGATACTGCAAC+CGG | - | Chr5:567162-567181 | None:intergenic | 45.0% | |
| GATGGTACTTGGAAACCATG+GGG | + | Chr5:567044-567063 | MsG0580024080.01.T01:CDS | 45.0% | |
| GTAAATCAAACCCAGGTGCT+TGG | + | Chr5:567000-567019 | MsG0580024080.01.T01:CDS | 45.0% | |
| GTGATGGTACTTGGAAACCA+TGG | + | Chr5:567042-567061 | MsG0580024080.01.T01:CDS | 45.0% | |
| TAACACTCCAAACGGAAGCT+GGG | + | Chr5:567229-567248 | MsG0580024080.01.T01:CDS | 45.0% | |
| TCCAACGGAGTTTGAGTAAC+CGG | - | Chr5:567093-567112 | None:intergenic | 45.0% | |
| TTAACACTCCAAACGGAAGC+TGG | + | Chr5:567228-567247 | MsG0580024080.01.T01:CDS | 45.0% | |
| TTACTGACCTGTTGTGAACC+AGG | - | Chr5:566984-567003 | None:intergenic | 45.0% | |
| TTCCAAGTACCATCACCATC+GGG | - | Chr5:567038-567057 | None:intergenic | 45.0% | |
| ! | CTTCCGTTTGGAGTGTTAAC+GGG | - | Chr5:567227-567246 | None:intergenic | 45.0% |
| ! | GCTTCCGTTTGGAGTGTTAA+CGG | - | Chr5:567228-567247 | None:intergenic | 45.0% |
| AACGTCGAACCGGTATCCAA+CGG | - | Chr5:567108-567127 | None:intergenic | 50.0% | |
| ACCATTGAAGCACACGCAAC+CGG | - | Chr5:566942-566961 | None:intergenic | 50.0% | |
| ACCTGTTGTGAACCAGGTGA+AGG | - | Chr5:566978-566997 | None:intergenic | 50.0% | |
| AGTAAACCGGAGGTGGAAGT+TGG | + | Chr5:567374-567393 | MsG0580024080.01.T01:CDS | 50.0% | |
| CCAAACGGAAGCTGGGATTT+AGG | + | Chr5:567236-567255 | MsG0580024080.01.T01:CDS | 50.0% | |
| CCAACGGAGTTTGAGTAACC+GGG | - | Chr5:567092-567111 | None:intergenic | 50.0% | |
| CCTAAATCCCAGCTTCCGTT+TGG | - | Chr5:567239-567258 | None:intergenic | 50.0% | |
| GAACCAGGTGAAGGAACGAA+CGG | - | Chr5:566969-566988 | None:intergenic | 50.0% | |
| GTGATGTCGGCGAGATTTGA+CGG | + | Chr5:567338-567357 | MsG0580024080.01.T01:CDS | 50.0% | |
| TCCTTCACCTGGTTCACAAC+AGG | + | Chr5:566974-566993 | MsG0580024080.01.T01:CDS | 50.0% | |
| TGTGAAGCACGTGACATGCA+CGG | + | Chr5:567397-567416 | MsG0580024080.01.T01:CDS | 50.0% | |
| TGTTGCTCTTGCAGCTGCTA+TGG | + | Chr5:567436-567455 | MsG0580024080.01.T01:CDS | 50.0% | |
| TTCACACCAACTTCCACCTC+CGG | - | Chr5:567383-567402 | None:intergenic | 50.0% | |
| ! | GGAAGCTGGGATTTAGGATC+CGG | + | Chr5:567242-567261 | MsG0580024080.01.T01:CDS | 50.0% |
| ! | TGGGATTTAGGATCCGGTTC+AGG | + | Chr5:567248-567267 | MsG0580024080.01.T01:CDS | 50.0% |
| !! | AACCCGATGGTGATGGTACT+TGG | + | Chr5:567033-567052 | MsG0580024080.01.T01:CDS | 50.0% |
| !! | ATACCGGTTCGACGTTCTTC+CGG | + | Chr5:567112-567131 | MsG0580024080.01.T01:CDS | 50.0% |
| ACACCGTTCGTTCCTTCACC+TGG | + | Chr5:566963-566982 | MsG0580024080.01.T01:CDS | 55.0% | |
| ACCGGTTGCGTGTGCTTCAA+TGG | + | Chr5:566938-566957 | MsG0580024080.01.T01:CDS | 55.0% | |
| ATCTGAACCGGAACCAGACC+CGG | - | Chr5:567282-567301 | None:intergenic | 55.0% | |
| CCCGGTTACTCAAACTCCGT+TGG | + | Chr5:567089-567108 | MsG0580024080.01.T01:CDS | 55.0% | |
| TCCGGTTCAGGATCAAGACC+CGG | + | Chr5:567260-567279 | MsG0580024080.01.T01:CDS | 55.0% | |
| AGCACACGCAACCGGTGAAC+CGG | - | Chr5:566934-566953 | None:intergenic | 60.0% | |
| CAAGCTTCGAGTCGACCCCA+TGG | - | Chr5:567062-567081 | None:intergenic | 60.0% | |
| CCCGGGTCTTGATCCTGAAC+CGG | - | Chr5:567264-567283 | None:intergenic | 60.0% | |
| CCGGTTCAGGATCAAGACCC+GGG | + | Chr5:567261-567280 | MsG0580024080.01.T01:CDS | 60.0% | |
| CTCGAAGCTTGGCGTGAACC+CGG | + | Chr5:567071-567090 | MsG0580024080.01.T01:CDS | 60.0% | |
| GAGTGTGACTGGATCAGCGG+TGG | - | Chr5:567138-567157 | None:intergenic | 60.0% | |
| GCTGATCCAGTCACACTCGC+CGG | + | Chr5:567140-567159 | MsG0580024080.01.T01:CDS | 60.0% | |
| GGCGAGTGTGACTGGATCAG+CGG | - | Chr5:567141-567160 | None:intergenic | 60.0% | |
| TCAGGATCAAGACCCGGGTC+TGG | + | Chr5:567266-567285 | MsG0580024080.01.T01:CDS | 60.0% | |
| TCTGAACCGGAACCAGACCC+GGG | - | Chr5:567281-567300 | None:intergenic | 60.0% | |
| TGGCCGGAAGAACGTCGAAC+CGG | - | Chr5:567118-567137 | None:intergenic | 60.0% | |
| ! | TCAAGACCCGGGTCTGGTTC+CGG | + | Chr5:567272-567291 | MsG0580024080.01.T01:CDS | 60.0% |
| !! | CATGGGGTCGACTCGAAGCT+TGG | + | Chr5:567060-567079 | MsG0580024080.01.T01:CDS | 60.0% |
| CTGCAACCGGCGAGTGTGAC+TGG | - | Chr5:567149-567168 | None:intergenic | 65.0% | |
| GTGACTGGATCAGCGGTGGC+CGG | - | Chr5:567134-567153 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 566891 | 567520 | 566891 | ID=MsG0580024080.01;Name=MsG0580024080.01 |
| Chr5 | mRNA | 566891 | 567520 | 566891 | ID=MsG0580024080.01.T01;Parent=MsG0580024080.01;Name=MsG0580024080.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|209 |
| Chr5 | exon | 566891 | 567520 | 566891 | ID=MsG0580024080.01.T01:exon:168;Parent=MsG0580024080.01.T01 |
| Chr5 | CDS | 566891 | 567520 | 566891 | ID=MsG0580024080.01.T01:cds;Parent=MsG0580024080.01.T01 |
| Gene Sequence |
| Protein sequence |
