AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024308.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024308.01.T01 MTR_5g008720 98.400 250 4 0 1 250 1 250 0.0 517
MsG0580024308.01.T01 MTR_5g008720 95.294 170 4 1 1 166 1 170 2.15e-117 334
MsG0580024308.01.T01 MTR_2g013780 35.176 199 125 2 28 222 70 268 4.94e-42 145
MsG0580024308.01.T01 MTR_4g088770 34.483 232 140 6 12 240 68 290 9.57e-42 145
MsG0580024308.01.T01 MTR_4g078310 40.331 181 105 3 44 222 66 245 3.01e-40 140
MsG0580024308.01.T01 MTR_4g088760 32.759 232 143 5 12 240 122 343 5.73e-40 142
MsG0580024308.01.T01 MTR_2g013880 30.973 226 149 3 18 240 65 286 2.19e-39 139
MsG0580024308.01.T01 MTR_4g132963 32.673 202 111 6 45 243 76 255 2.59e-30 114
MsG0580024308.01.T01 MTR_4g132940 32.673 202 111 6 45 243 76 255 2.59e-30 114
MsG0580024308.01.T01 MTR_4g132970 32.512 203 113 6 45 243 94 276 2.14e-29 112
MsG0580024308.01.T01 MTR_7g083080 29.697 165 105 4 76 240 117 270 7.81e-28 108
MsG0580024308.01.T01 MTR_1g041155 34.677 124 75 1 97 220 153 270 2.27e-24 100
MsG0580024308.01.T01 MTR_2g025520 36.290 124 73 1 97 220 134 251 4.36e-24 98.6
MsG0580024308.01.T01 MTR_8g093440 33.594 128 84 1 94 220 79 206 9.91e-24 96.7
MsG0580024308.01.T01 MTR_8g093440 33.594 128 84 1 94 220 17 144 1.02e-23 95.1
MsG0580024308.01.T01 MTR_1g076500 33.333 141 83 2 97 232 175 309 9.59e-23 95.9
MsG0580024308.01.T01 MTR_1g070970 28.646 192 120 4 29 220 111 285 2.79e-22 94.4
MsG0580024308.01.T01 MTR_8g012240 31.544 149 95 3 99 241 133 280 1.69e-21 91.3
MsG0580024308.01.T01 MTR_7g053310 34.109 129 83 2 95 222 118 245 1.95e-21 90.9
MsG0580024308.01.T01 MTR_7g095960 32.812 128 78 3 98 225 161 280 1.87e-19 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024308.01.T01 AT4G32480 36.548 197 123 2 28 222 45 241 1.55e-46 157
MsG0580024308.01.T01 AT2G20670 35.096 208 130 3 44 249 63 267 2.44e-43 149
MsG0580024308.01.T01 AT3G07350 35.714 126 81 0 97 222 135 260 6.89e-30 114
MsG0580024308.01.T01 AT3G25240 38.760 129 78 1 95 222 113 241 1.37e-28 110
MsG0580024308.01.T01 AT2G39650 33.071 127 85 0 94 220 45 171 1.33e-26 103
MsG0580024308.01.T01 AT2G39650 33.071 127 85 0 94 220 107 233 1.97e-26 104
MsG0580024308.01.T01 AT4G14620 34.677 124 75 1 97 220 143 260 8.93e-26 103
MsG0580024308.01.T01 AT2G38820 36.508 126 73 2 95 220 158 276 1.20e-24 100
MsG0580024308.01.T01 AT1G12030 33.333 171 93 5 52 222 81 230 1.54e-24 99.4
MsG0580024308.01.T01 AT3G22970 33.871 124 76 1 97 220 57 174 3.88e-23 95.1
MsG0580024308.01.T01 AT3G22970 33.871 124 76 1 97 220 168 285 1.76e-22 95.1
MsG0580024308.01.T01 AT1G62420 32.540 126 82 1 98 223 109 231 7.97e-22 92.0
MsG0580024308.01.T01 AT3G54550 27.465 142 93 2 101 238 148 283 8.92e-17 78.2
MsG0580024308.01.T01 AT1G77145 29.545 132 81 2 101 220 79 210 8.73e-15 72.4
MsG0580024308.01.T01 AT1G77160 29.545 132 81 2 101 220 29 160 1.15e-14 70.9
MsG0580024308.01.T01 AT1G77160 29.545 132 81 2 101 220 84 215 1.56e-14 71.6
MsG0580024308.01.T01 AT1G77145 35.789 95 57 1 130 220 59 153 7.50e-14 68.6

Find 81 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTTGTGTTAGGAAATTT+TGG 0.191126 5:-3237296 MsG0580024308.01.T01:intron
TTTGACATTCATTTCCCTTT+AGG 0.255256 5:-3236312 MsG0580024308.01.T01:three_prime_UTR
TTTGCTTCAACCACTTTGTT+TGG 0.290431 5:-3238549 MsG0580024308.01.T01:five_prime_UTR
GCAAACAAGACACAAGTTTC+TGG 0.293899 5:+3236674 None:intergenic
AGTACCATTTCTGATTGAAT+TGG 0.335912 5:-3236740 MsG0580024308.01.T01:CDS
TCAAAATCTACCACTGGAAT+TGG 0.346284 5:+3236406 None:intergenic
AACACAAAGGACTTTGTATT+GGG 0.356331 5:-3238322 MsG0580024308.01.T01:CDS
AATACAAAGTCCTTTGTGTT+AGG 0.357106 5:+3238325 None:intergenic
GAATTTGCTTGTGTTGTTGA+AGG 0.360556 5:-3236106 MsG0580024308.01.T01:three_prime_UTR
GAGTCATGTGTCGCGTTTCT+AGG 0.366332 5:-3236171 MsG0580024308.01.T01:three_prime_UTR
GGCTTCCCAACATAAAATTC+AGG 0.380679 5:+3236652 None:intergenic
TAACACAAAGGACTTTGTAT+TGG 0.383816 5:-3238323 MsG0580024308.01.T01:CDS
TTGGAGCGACACCATATGAT+TGG 0.387836 5:-3237277 MsG0580024308.01.T01:CDS
GAAGGAGAAGAAAATGTATC+TGG 0.388065 5:-3236575 MsG0580024308.01.T01:CDS
ATCAGAGCTGCATGCCCCTC+TGG 0.391295 5:-3238391 MsG0580024308.01.T01:CDS
AATTCCAATTCAATCAGAAA+TGG 0.405930 5:+3236736 None:intergenic
TAGAAGCATGAAATTGAAAA+GGG 0.407876 5:+3236054 None:intergenic
GTAGAAGCATGAAATTGAAA+AGG 0.412944 5:+3236053 None:intergenic
TTCATGCAAATGAAGTGGTC+AGG 0.435123 5:-3236532 MsG0580024308.01.T01:CDS
GTGGTGCAATAATGAAAGTT+AGG 0.436885 5:+3237181 None:intergenic
TTGACTGCTACCACTGCATT+AGG 0.442412 5:+3236448 None:intergenic
CTACCAACCTTTGCACATCT+AGG 0.445506 5:-3237111 MsG0580024308.01.T01:CDS
AAAACCACAAATGAATTTCC+AGG 0.449507 5:-3237085 MsG0580024308.01.T01:CDS
GGAATATATTATAAAGCATA+TGG 0.454576 5:-3236344 MsG0580024308.01.T01:three_prime_UTR
TAGATTCACTTGCTTGTGTA+TGG 0.461506 5:+3236491 None:intergenic
TGGAGCGACACCATATGATT+GGG 0.464808 5:-3237276 MsG0580024308.01.T01:CDS
AGAAAGACAAAGAGGGAAGA+AGG 0.470745 5:+3238509 None:intergenic
AGCCACTAGTCTTCTCAAAC+AGG 0.471489 5:+3237159 None:intergenic
TAACATAACATAGGACCTAA+AGG 0.473739 5:+3236297 None:intergenic
ACCTCCTGGAAATTCATTTG+TGG 0.474315 5:+3237081 None:intergenic
AGAAAATGTATCTGGCTCCT+TGG 0.483912 5:-3236567 MsG0580024308.01.T01:CDS
TCAATTTCGACCCAATCATA+TGG 0.486618 5:+3237266 None:intergenic
AACATAACATAGGACCTAAA+GGG 0.488803 5:+3236298 None:intergenic
AAATGTATCTGGCTCCTTGG+AGG 0.492634 5:-3236564 MsG0580024308.01.T01:CDS
TATTGTGTTGTTGAAGAGCA+TGG 0.504095 5:-3238454 MsG0580024308.01.T01:exon
GCTTGTGTATGGTGATATGT+TGG 0.515521 5:+3236502 None:intergenic
TCCCCTTCAAAATCTACCAC+TGG 0.525171 5:+3236400 None:intergenic
TGCTTTGTTCAGACAAGCAT+AGG 0.542553 5:+3238364 None:intergenic
CAAAGAGGGAAGAAGGGCTA+AGG 0.542597 5:+3238516 None:intergenic
ACAATCGCGGTTAAGTACTC+TGG 0.547258 5:+3236631 None:intergenic
GAAAGACAAAGAGGGAAGAA+GGG 0.549864 5:+3238510 None:intergenic
ACACAATAGAAACTACTACT+AGG 0.551480 5:+3236254 None:intergenic
AAACAAAGTGGTTGAAGCAA+AGG 0.555672 5:+3238551 None:intergenic
CCACCTAGATGTGCAAAGGT+TGG 0.561006 5:+3237108 None:intergenic
TGCTGCAAAGAAATCAATGA+AGG 0.567461 5:-3236593 MsG0580024308.01.T01:CDS
CAGTTCCAGATTGCAAAAGC+AGG 0.571376 5:-3236706 MsG0580024308.01.T01:CDS
GCATGAAATTGAAAAGGGGA+AGG 0.575445 5:+3236059 None:intergenic
AGGGGAAGGAAGAATGCAAA+GGG 0.579820 5:+3236073 None:intergenic
GCAGTTTCATGCAAATGAAG+TGG 0.583111 5:-3236537 MsG0580024308.01.T01:CDS
ATTCGTTTAGAAAGACAAAG+AGG 0.584945 5:+3238501 None:intergenic
ATTACATCATAACATAACAT+AGG 0.585846 5:+3236288 None:intergenic
GCTGCTATGCTATGTGAAAG+AGG 0.587932 5:-3237139 MsG0580024308.01.T01:CDS
GACAAGCATAGGTCCTCCAG+AGG 0.591272 5:+3238375 None:intergenic
ATTCCTACGTACTAATGAAG+AGG 0.597235 5:+3236362 None:intergenic
TTCAGGAGCTCCTAATGCAG+TGG 0.598514 5:-3236458 MsG0580024308.01.T01:CDS
GCAAGTAAGTTTCACAATGG+AGG 0.598818 5:-3236217 MsG0580024308.01.T01:three_prime_UTR
AAAGCAAGTAAGTTTCACAA+TGG 0.612119 5:-3236220 MsG0580024308.01.T01:three_prime_UTR
AGAGCTGCATGCCCCTCTGG+AGG 0.612588 5:-3238388 MsG0580024308.01.T01:CDS
TTCTTCAATTCCAATTCCAG+TGG 0.613919 5:-3236416 MsG0580024308.01.T01:three_prime_UTR
TTCTAAACGAATAACAAAGA+GGG 0.613986 5:-3238490 MsG0580024308.01.T01:five_prime_UTR
TTTCTAAACGAATAACAAAG+AGG 0.615612 5:-3238491 MsG0580024308.01.T01:five_prime_UTR
TTCGTTTAGAAAGACAAAGA+GGG 0.620609 5:+3238502 None:intergenic
TCGAAATTGAGACAAGAGGT+TGG 0.623564 5:-3237253 MsG0580024308.01.T01:CDS
TGGGTCGAAATTGAGACAAG+AGG 0.625191 5:-3237257 MsG0580024308.01.T01:CDS
CCAACCTTTGCACATCTAGG+TGG 0.637798 5:-3237108 MsG0580024308.01.T01:CDS
AGAGAAAGTGCCAAACAAAG+TGG 0.639651 5:+3238539 None:intergenic
TGAACAAAGCAATGACTCTG+AGG 0.640823 5:-3238352 MsG0580024308.01.T01:CDS
CACCTGTTTGAGAAGACTAG+TGG 0.640946 5:-3237161 MsG0580024308.01.T01:CDS
AGAAGCATGAAATTGAAAAG+GGG 0.641184 5:+3236055 None:intergenic
AAGGGGAAGGAAGAATGCAA+AGG 0.644340 5:+3236072 None:intergenic
CACTAGTCTTCTCAAACAGG+TGG 0.650169 5:+3237162 None:intergenic
ATCTCAGCTAGCACTTCTTG+AGG 0.650841 5:-3238298 MsG0580024308.01.T01:intron
ACACACAAGTCGAACAATCG+CGG 0.653266 5:+3236618 None:intergenic
ATGTTGTCAACAGAGAGTAG+AGG 0.653934 5:-3236135 MsG0580024308.01.T01:three_prime_UTR
ACAAGCATAGGTCCTCCAGA+GGG 0.654210 5:+3238376 None:intergenic
CAATAGAAACTACTACTAGG+TGG 0.667165 5:+3236257 None:intergenic
AGACCTCTTCATTAGTACGT+AGG 0.670566 5:-3236365 MsG0580024308.01.T01:three_prime_UTR
TCTCTGTTGACAACATCTAG+TGG 0.677328 5:+3236142 None:intergenic
CTGAGGAACACCTAACACAA+AGG 0.685583 5:-3238335 MsG0580024308.01.T01:CDS
CAAGCATAGGTCCTCCAGAG+GGG 0.712265 5:+3238377 None:intergenic
ACCACAAATGAATTTCCAGG+AGG 0.741742 5:-3237082 MsG0580024308.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTATTTTATTTTATTT+TGG + Chr5:3237016-3237035 None:intergenic 0.0%
!! TTTATAATAACTAACTATTT+AGG + Chr5:3237586-3237605 None:intergenic 10.0%
!!! ACAAAATAATGAATTTTTAT+TGG - Chr5:3237708-3237727 MsG0580024308.01.T01:intron 10.0%
!!! AGTAATTAATTTTAGATTAA+AGG + Chr5:3237743-3237762 None:intergenic 10.0%
!!! TAAAATCATTTTATGTAATT+AGG - Chr5:3237034-3237053 MsG0580024308.01.T01:intron 10.0%
!! ATAAAATAAATCTTTGATGT+TGG - Chr5:3238598-3238617 MsG0580024308.01.T01:five_prime_UTR 15.0%
!! ATCTTTAAACTAATAGAATA+TGG - Chr5:3236639-3236658 MsG0580024308.01.T01:CDS 15.0%
!! ATTTGATTATGATTATGAAA+TGG - Chr5:3237242-3237261 MsG0580024308.01.T01:CDS 15.0%
!! TAGTTAGTTATTATAAAGTT+TGG - Chr5:3237589-3237608 MsG0580024308.01.T01:intron 15.0%
!!! TTAATTACTATTTTACACTA+TGG - Chr5:3237754-3237773 MsG0580024308.01.T01:intron 15.0%
!!! TTTAAAGAATTCATTTTCAT+TGG + Chr5:3236824-3236843 None:intergenic 15.0%
!!! TTTTATTTTTCAAGATATAG+TGG + Chr5:3237276-3237295 None:intergenic 15.0%
!! AATAACTAACTATTTAGGTA+AGG + Chr5:3237581-3237600 None:intergenic 20.0%
!! ATTACATCATAACATAACAT+AGG + Chr5:3238324-3238343 None:intergenic 20.0%
!! GGAATATATTATAAAGCATA+TGG - Chr5:3238265-3238284 MsG0580024308.01.T01:intron 20.0%
!! TAAAAATTATAATGCGTCAA+AGG + Chr5:3237659-3237678 None:intergenic 20.0%
!! TTAAAAGCAATTATTCAATG+TGG + Chr5:3236998-3237017 None:intergenic 20.0%
!! TTAATCAAGCACATAATATT+TGG + Chr5:3236540-3236559 None:intergenic 20.0%
!!! AGTTTTACTATTTTCAAACA+TGG - Chr5:3236779-3236798 MsG0580024308.01.T01:CDS 20.0%
!!! CATTTTATGTAATTAGGTAT+TGG - Chr5:3237040-3237059 MsG0580024308.01.T01:intron 20.0%
!!! TATTATTTTTTTGACCAAGT+TGG - Chr5:3236683-3236702 MsG0580024308.01.T01:CDS 20.0%
!!! TCTTGGATATTTTTAATTTG+TGG - Chr5:3237082-3237101 MsG0580024308.01.T01:intron 20.0%
!!! TTATTTGTGTTAGGAAATTT+TGG - Chr5:3237313-3237332 MsG0580024308.01.T01:intron 20.0%
! AAAATCAAATAGAGCCAATT+TGG - Chr5:3236404-3236423 MsG0580024308.01.T01:three_prime_UTR 25.0%
! AATTCCAATTCAATCAGAAA+TGG + Chr5:3237876-3237895 None:intergenic 25.0%
! AGTTATGTCATTGTAAAATG+TGG + Chr5:3237620-3237639 None:intergenic 25.0%
! ATATCCAAGAATATCCTTAA+AGG + Chr5:3237072-3237091 None:intergenic 25.0%
! ATGTTTAATTCAAACTCCAT+GGG + Chr5:3236738-3236757 None:intergenic 25.0%
! GTTGTCATAATTTCATATGT+TGG + Chr5:3237135-3237154 None:intergenic 25.0%
! TCAAATACTACATTTGTATG+TGG + Chr5:3237228-3237247 None:intergenic 25.0%
! TTCTAAACGAATAACAAAGA+GGG - Chr5:3236119-3236138 MsG0580024308.01.T01:three_prime_UTR 25.0%
! TTTCTAAACGAATAACAAAG+AGG - Chr5:3236118-3236137 MsG0580024308.01.T01:three_prime_UTR 25.0%
!! CTTTTTGCATTATTTGTGTT+AGG - Chr5:3237304-3237323 MsG0580024308.01.T01:intron 25.0%
!! TAGAAGCATGAAATTGAAAA+GGG + Chr5:3238558-3238577 None:intergenic 25.0%
!! TATTCCTTTAAGGATATTCT+TGG - Chr5:3237065-3237084 MsG0580024308.01.T01:intron 25.0%
!!! AGTAAAACTGATTTTACCAA+GGG + Chr5:3236768-3236787 None:intergenic 25.0%
!!! GGCTCTATTTGATTTTAATT+TGG + Chr5:3236400-3236419 None:intergenic 25.0%
!!! TAGTAAAACTGATTTTACCA+AGG + Chr5:3236769-3236788 None:intergenic 25.0%
AAAACCACAAATGAATTTCC+AGG - Chr5:3237524-3237543 MsG0580024308.01.T01:intron 30.0%
AAAGCAAGTAAGTTTCACAA+TGG - Chr5:3238389-3238408 MsG0580024308.01.T01:CDS 30.0%
AACACAAAGGACTTTGTATT+GGG - Chr5:3236287-3236306 MsG0580024308.01.T01:three_prime_UTR 30.0%
AACATAACATAGGACCTAAA+GGG + Chr5:3238314-3238333 None:intergenic 30.0%
AATACAAAGTCCTTTGTGTT+AGG + Chr5:3236287-3236306 None:intergenic 30.0%
ACACAATAGAAACTACTACT+AGG + Chr5:3238358-3238377 None:intergenic 30.0%
ACATCAACAATAAACCAACT+TGG + Chr5:3236700-3236719 None:intergenic 30.0%
ACTAACTATTTAGGTAAGGT+AGG + Chr5:3237577-3237596 None:intergenic 30.0%
AGAATATGGTCATATCATGT+TGG - Chr5:3236653-3236672 MsG0580024308.01.T01:CDS 30.0%
AGTACCATTTCTGATTGAAT+TGG - Chr5:3237869-3237888 MsG0580024308.01.T01:intron 30.0%
ATCAATGCCACTAGATATTT+CGG + Chr5:3236858-3236877 None:intergenic 30.0%
ATTAAACATGAAGAAACCCT+TGG - Chr5:3236749-3236768 MsG0580024308.01.T01:CDS 30.0%
ATTCGTTTAGAAAGACAAAG+AGG + Chr5:3236111-3236130 None:intergenic 30.0%
CATGGCAAAAAAATAGAACA+AGG - Chr5:3236797-3236816 MsG0580024308.01.T01:intron 30.0%
CATGTTTAATTCAAACTCCA+TGG + Chr5:3236739-3236758 None:intergenic 30.0%
TAACACAAAGGACTTTGTAT+TGG - Chr5:3236286-3236305 MsG0580024308.01.T01:three_prime_UTR 30.0%
TAACATAACATAGGACCTAA+AGG + Chr5:3238315-3238334 None:intergenic 30.0%
TCACATGAATAAATTACCCA+TGG - Chr5:3236719-3236738 MsG0580024308.01.T01:CDS 30.0%
TGAAGCTTGATGTTATGATA+AGG + Chr5:3236478-3236497 None:intergenic 30.0%
TTCGTTTAGAAAGACAAAGA+GGG + Chr5:3236110-3236129 None:intergenic 30.0%
TTTGACATTCATTTCCCTTT+AGG - Chr5:3238297-3238316 MsG0580024308.01.T01:intron 30.0%
! AGAAGCATGAAATTGAAAAG+GGG + Chr5:3238557-3238576 None:intergenic 30.0%
! ATTTTTCCCGAAATATCTAG+TGG - Chr5:3236848-3236867 MsG0580024308.01.T01:intron 30.0%
! GAGAATAGATGTTTTCTGTT+TGG - Chr5:3236904-3236923 MsG0580024308.01.T01:intron 30.0%
! GGTATTGGAATATTCCTTTA+AGG - Chr5:3237055-3237074 MsG0580024308.01.T01:intron 30.0%
! GTAGAAGCATGAAATTGAAA+AGG + Chr5:3238559-3238578 None:intergenic 30.0%
! GTTTGCCTGAATTTTATGTT+GGG - Chr5:3237952-3237971 MsG0580024308.01.T01:intron 30.0%
! TCATATGTTGGTTGAAGTAA+CGG + Chr5:3237123-3237142 None:intergenic 30.0%
! TGTTTGCCTGAATTTTATGT+TGG - Chr5:3237951-3237970 MsG0580024308.01.T01:intron 30.0%
! TTTATTTGTTTCTCCCTTGA+AGG - Chr5:3236562-3236581 MsG0580024308.01.T01:CDS 30.0%
!! TAGATTTTGAAGGGGAATTT+TGG - Chr5:3238216-3238235 MsG0580024308.01.T01:intron 30.0%
!! TTCTGTTTGGCTTCTTTTAT+TGG - Chr5:3236917-3236936 MsG0580024308.01.T01:intron 30.0%
!!! CTGATGTTGCTTTTGTTTTT+AGG - Chr5:3237795-3237814 MsG0580024308.01.T01:intron 30.0%
AAACAAAGTGGTTGAAGCAA+AGG + Chr5:3236061-3236080 None:intergenic 35.0%
AGAAAACATCTATTCTCGCA+AGG + Chr5:3236901-3236920 None:intergenic 35.0%
AGCTTGATGTTATGATAAGG+AGG + Chr5:3236475-3236494 None:intergenic 35.0%
ATATTGATATATGCACCTCC+TGG + Chr5:3237545-3237564 None:intergenic 35.0%
ATTCCTACGTACTAATGAAG+AGG + Chr5:3238250-3238269 None:intergenic 35.0%
CAATAGAAACTACTACTAGG+TGG + Chr5:3238355-3238374 None:intergenic 35.0%
GAAGGAGAAGAAAATGTATC+TGG - Chr5:3238034-3238053 MsG0580024308.01.T01:intron 35.0%
GTGGTGCAATAATGAAAGTT+AGG + Chr5:3237431-3237450 None:intergenic 35.0%
TAGATTCACTTGCTTGTGTA+TGG + Chr5:3238121-3238140 None:intergenic 35.0%
TCAAAATCTACCACTGGAAT+TGG + Chr5:3238206-3238225 None:intergenic 35.0%
TCAATGCCACTAGATATTTC+GGG + Chr5:3236857-3236876 None:intergenic 35.0%
TCAATTTCGACCCAATCATA+TGG + Chr5:3237346-3237365 None:intergenic 35.0%
TGCTGCAAAGAAATCAATGA+AGG - Chr5:3238016-3238035 MsG0580024308.01.T01:intron 35.0%
TTCTTCAATTCCAATTCCAG+TGG - Chr5:3238193-3238212 MsG0580024308.01.T01:intron 35.0%
TTTGCTTCAACCACTTTGTT+TGG - Chr5:3236060-3236079 MsG0580024308.01.T01:three_prime_UTR 35.0%
! AGAAAAAGTGTGCGTGTTTA+TGG - Chr5:3236188-3236207 MsG0580024308.01.T01:three_prime_UTR 35.0%
! ATAGAGCCAATTTGGTTTCT+TGG - Chr5:3236412-3236431 MsG0580024308.01.T01:three_prime_UTR 35.0%
! ATTCCAGTGGTAGATTTTGA+AGG - Chr5:3238206-3238225 MsG0580024308.01.T01:intron 35.0%
! CATATGTTGGTTGAAGTAAC+GGG + Chr5:3237122-3237141 None:intergenic 35.0%
! GAATCTAACATGAGCTTTTC+AGG - Chr5:3238134-3238153 MsG0580024308.01.T01:intron 35.0%
! GAATTTGCTTGTGTTGTTGA+AGG - Chr5:3238503-3238522 MsG0580024308.01.T01:five_prime_UTR 35.0%
! TATTGTGTTGTTGAAGAGCA+TGG - Chr5:3236155-3236174 MsG0580024308.01.T01:three_prime_UTR 35.0%
! TTCCAGTGGTAGATTTTGAA+GGG - Chr5:3238207-3238226 MsG0580024308.01.T01:intron 35.0%
!! TGTTTGGCTTCTTTTATTGG+TGG - Chr5:3236920-3236939 MsG0580024308.01.T01:intron 35.0%
ACCACAAATGAATTTCCAGG+AGG - Chr5:3237527-3237546 MsG0580024308.01.T01:intron 40.0%
ACCTCCTGGAAATTCATTTG+TGG + Chr5:3237531-3237550 None:intergenic 40.0%
AGAAAGACAAAGAGGGAAGA+AGG + Chr5:3236103-3236122 None:intergenic 40.0%
AGACCTCTTCATTAGTACGT+AGG - Chr5:3238244-3238263 MsG0580024308.01.T01:intron 40.0%
AGCCAAGATCAAATCCTTCA+AGG + Chr5:3236579-3236598 None:intergenic 40.0%
ATGTTGTCAACAGAGAGTAG+AGG - Chr5:3238474-3238493 MsG0580024308.01.T01:five_prime_UTR 40.0%
GAAAGACAAAGAGGGAAGAA+GGG + Chr5:3236102-3236121 None:intergenic 40.0%
GCAAACAAGACACAAGTTTC+TGG + Chr5:3237938-3237957 None:intergenic 40.0%
GCAAGTAAGTTTCACAATGG+AGG - Chr5:3238392-3238411 MsG0580024308.01.T01:CDS 40.0%
GCAGTTTCATGCAAATGAAG+TGG - Chr5:3238072-3238091 MsG0580024308.01.T01:intron 40.0%
GCATGAAATTGAAAAGGGGA+AGG + Chr5:3238553-3238572 None:intergenic 40.0%
GCCAAGATCAAATCCTTCAA+GGG + Chr5:3236578-3236597 None:intergenic 40.0%
GCTTGTGTATGGTGATATGT+TGG + Chr5:3238110-3238129 None:intergenic 40.0%
GGCTTCCCAACATAAAATTC+AGG + Chr5:3237960-3237979 None:intergenic 40.0%
TCCCTTGAAGGATTTGATCT+TGG - Chr5:3236574-3236593 MsG0580024308.01.T01:CDS 40.0%
TCGAAATTGAGACAAGAGGT+TGG - Chr5:3237356-3237375 MsG0580024308.01.T01:intron 40.0%
TCTCTGTTGACAACATCTAG+TGG + Chr5:3238470-3238489 None:intergenic 40.0%
TGAACAAAGCAATGACTCTG+AGG - Chr5:3236257-3236276 MsG0580024308.01.T01:three_prime_UTR 40.0%
TGCATGCCAAGAAACCAAAT+TGG + Chr5:3236421-3236440 None:intergenic 40.0%
TGGAGGAAAGTCGAAAAACT+CGG - Chr5:3238409-3238428 MsG0580024308.01.T01:CDS 40.0%
TTCATGCAAATGAAGTGGTC+AGG - Chr5:3238077-3238096 MsG0580024308.01.T01:intron 40.0%
! AGAAAATGTATCTGGCTCCT+TGG - Chr5:3238042-3238061 MsG0580024308.01.T01:intron 40.0%
! TGCTTTGTTCAGACAAGCAT+AGG + Chr5:3236248-3236267 None:intergenic 40.0%
! TTTTCCACCTAGATGTGCAA+AGG + Chr5:3237508-3237527 None:intergenic 40.0%
!! AGAGAAAGTGCCAAACAAAG+TGG + Chr5:3236073-3236092 None:intergenic 40.0%
!! ATCTAGTGGCATTGATCTAG+TGG - Chr5:3236862-3236881 MsG0580024308.01.T01:intron 40.0%
!! ATGAAACTGCTTTTCCTCCA+AGG + Chr5:3238062-3238081 None:intergenic 40.0%
!! TCCAGTGGTAGATTTTGAAG+GGG - Chr5:3238208-3238227 MsG0580024308.01.T01:intron 40.0%
AAGGGGAAGGAAGAATGCAA+AGG + Chr5:3238540-3238559 None:intergenic 45.0%
ACAATCGCGGTTAAGTACTC+TGG + Chr5:3237981-3238000 None:intergenic 45.0%
ACACACAAGTCGAACAATCG+CGG + Chr5:3237994-3238013 None:intergenic 45.0%
AGGAAAGTCGAAAAACTCGG+TGG - Chr5:3238412-3238431 MsG0580024308.01.T01:CDS 45.0%
AGGGGAAGGAAGAATGCAAA+GGG + Chr5:3238539-3238558 None:intergenic 45.0%
ATCTCAGCTAGCACTTCTTG+AGG - Chr5:3236311-3236330 MsG0580024308.01.T01:three_prime_UTR 45.0%
CACCTGTTTGAGAAGACTAG+TGG - Chr5:3237448-3237467 MsG0580024308.01.T01:intron 45.0%
CAGTTCCAGATTGCAAAAGC+AGG - Chr5:3237903-3237922 MsG0580024308.01.T01:intron 45.0%
CTACCAACCTTTGCACATCT+AGG - Chr5:3237498-3237517 MsG0580024308.01.T01:intron 45.0%
CTGAGGAACACCTAACACAA+AGG - Chr5:3236274-3236293 MsG0580024308.01.T01:three_prime_UTR 45.0%
GCTGCTATGCTATGTGAAAG+AGG - Chr5:3237470-3237489 MsG0580024308.01.T01:intron 45.0%
TCCCCTTCAAAATCTACCAC+TGG + Chr5:3238212-3238231 None:intergenic 45.0%
TGGAGCGACACCATATGATT+GGG - Chr5:3237333-3237352 MsG0580024308.01.T01:intron 45.0%
TGGGTCGAAATTGAGACAAG+AGG - Chr5:3237352-3237371 MsG0580024308.01.T01:intron 45.0%
TTGACTGCTACCACTGCATT+AGG + Chr5:3238164-3238183 None:intergenic 45.0%
TTGGAGCGACACCATATGAT+TGG - Chr5:3237332-3237351 MsG0580024308.01.T01:intron 45.0%
! AAATGTATCTGGCTCCTTGG+AGG - Chr5:3238045-3238064 MsG0580024308.01.T01:intron 45.0%
!! AGCCACTAGTCTTCTCAAAC+AGG + Chr5:3237453-3237472 None:intergenic 45.0%
!! CACTAGTCTTCTCAAACAGG+TGG + Chr5:3237450-3237469 None:intergenic 45.0%
!! CTTCACCTGCTTTTGCAATC+TGG + Chr5:3237911-3237930 None:intergenic 45.0%
ACAAGCATAGGTCCTCCAGA+GGG + Chr5:3236236-3236255 None:intergenic 50.0%
CAAAGAGGGAAGAAGGGCTA+AGG + Chr5:3236096-3236115 None:intergenic 50.0%
CCAACCTTTGCACATCTAGG+TGG - Chr5:3237501-3237520 MsG0580024308.01.T01:intron 50.0%
CCACCTAGATGTGCAAAGGT+TGG + Chr5:3237504-3237523 None:intergenic 50.0%
GAGTCATGTGTCGCGTTTCT+AGG - Chr5:3238438-3238457 MsG0580024308.01.T01:CDS 50.0%
TTCAGGAGCTCCTAATGCAG+TGG - Chr5:3238151-3238170 MsG0580024308.01.T01:intron 50.0%
CAAGCATAGGTCCTCCAGAG+GGG + Chr5:3236235-3236254 None:intergenic 55.0%
GACAAGCATAGGTCCTCCAG+AGG + Chr5:3236237-3236256 None:intergenic 55.0%
ATCAGAGCTGCATGCCCCTC+TGG - Chr5:3236218-3236237 MsG0580024308.01.T01:three_prime_UTR 60.0%
AGAGCTGCATGCCCCTCTGG+AGG - Chr5:3236221-3236240 MsG0580024308.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 3236012 3238619 3236012 ID=MsG0580024308.01;Name=MsG0580024308.01
Chr5 mRNA 3236012 3238619 3236012 ID=MsG0580024308.01.T01;Parent=MsG0580024308.01;Name=MsG0580024308.01.T01;_AED=0.25;_eAED=0.25;_QI=162|1|1|1|1|1|3|432|250
Chr5 exon 3236012 3236814 3236012 ID=MsG0580024308.01.T01:exon:2001;Parent=MsG0580024308.01.T01
Chr5 exon 3237083 3237305 3237083 ID=MsG0580024308.01.T01:exon:2000;Parent=MsG0580024308.01.T01
Chr5 exon 3238299 3238619 3238299 ID=MsG0580024308.01.T01:exon:1999;Parent=MsG0580024308.01.T01
Chr5 five_prime_UTR 3238458 3238619 3238458 ID=MsG0580024308.01.T01:five_prime_utr;Parent=MsG0580024308.01.T01
Chr5 CDS 3238299 3238457 3238299 ID=MsG0580024308.01.T01:cds;Parent=MsG0580024308.01.T01
Chr5 CDS 3237083 3237305 3237083 ID=MsG0580024308.01.T01:cds;Parent=MsG0580024308.01.T01
Chr5 CDS 3236444 3236814 3236444 ID=MsG0580024308.01.T01:cds;Parent=MsG0580024308.01.T01
Chr5 three_prime_UTR 3236012 3236443 3236012 ID=MsG0580024308.01.T01:three_prime_utr;Parent=MsG0580024308.01.T01
Gene Sequence

>MsG0580024308.01.T01

ATGGAAAGCTTTGAAGAAAAAGTGTGCGTGTTTATGGATATTGAATCAGAGCTGCATGCCCCTCTGGAGGACCTATGCTTGTCTGAACAAAGCAATGACTCTGAGGAACACCTAACACAAAGGACTTTGTATTGGGAATCTCAGCTAGCACTTCTTGAGGAAATTTTGGAGCGACACCATATGATTGGGTCGAAATTGAGACAAGAGGTTGGTAGAATCATAGACAATATCAAAGCTTCGAATTATTGCAGCTGCTTCAAGCCTAACTTTCATTATTGCACCACCTGTTTGAGAAGACTAGTGGCTGCTATGCTATGTGAAAGAGGATTCACTACCAACCTTTGCACATCTAGGTGGAAAACCACAAATGAATTTCCAGGAGGCTCGCACGAGTACATTGAAGTGATTGCAAGCACAACAACAAGAAAGAAACAAGTACCATTTCTGATTGAATTGGAATTGAAAGAACAGTTCCAGATTGCAAAAGCAGGTGAAGAATACCAGAAACTTGTGTCTTGTTTGCCTGAATTTTATGTTGGGAAGCCAGAGTACTTAACCGCGATTGTTCGACTTGTGTGTGATGCTGCAAAGAAATCAATGAAGGAGAAGAAAATGTATCTGGCTCCTTGGAGGAAAAGCAGTTTCATGCAAATGAAGTGGTCAGGTTTCAATCCAACATATCACCATACACAAGCAAGTGAATCTAACATGAGCTTTTCAGGAGCTCCTAATGCAGTGGTAGCAGTCAACTGA
Protein sequence

>MsG0580024308.01.T01

MESFEEKVCVFMDIESELHAPLEDLCLSEQSNDSEEHLTQRTLYWESQLALLEEILERHHMIGSKLRQEVGRIIDNIKASNYCSCFKPNFHYCTTCLRRLVAAMLCERGFTTNLCTSRWKTTNEFPGGSHEYIEVIASTTTRKKQVPFLIELELKEQFQIAKAGEEYQKLVSCLPEFYVGKPEYLTAIVRLVCDAAKKSMKEKKMYLAPWRKSSFMQMKWSGFNPTYHHTQASESNMSFSGAPNAVVAVN*