AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680035612.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035612.01.T01 MTR_6g085050 100.000 58 0 0 1 58 103 160 1.33e-37 122
MsG0680035612.01.T01 MTR_5g054770 84.483 58 9 0 1 58 99 156 2.49e-30 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680035612.01.T01 AT1G68660 86.207 58 8 0 1 58 102 159 1.77e-31 107

Find 10 sgRNAs with CRISPR-Local

Find 8 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCAAGAAGCACATATTAA+TGG 0.300616 6:-109351918 MsG0680035612.01.T01:CDS
GCAATATCAACTGTCATTCC+AGG 0.427583 6:+109351951 None:intergenic
TGCATGCAATTGAGAGGAAA+TGG 0.433575 6:-109351849 MsG0680035612.01.T01:CDS
TTCCTCTCAATTGCATGCAA+TGG 0.502233 6:+109351853 None:intergenic
ACTCAACAACCACCATCAGC+TGG 0.532785 6:+109351807 None:intergenic
AGTTCAATTGAGCCAGCTGA+TGG 0.556313 6:-109351819 MsG0680035612.01.T01:CDS
GACCATTGCATGCAATTGAG+AGG 0.614338 6:-109351855 MsG0680035612.01.T01:CDS
GTGTTGATGAAAGTGATACC+TGG 0.634232 6:-109351969 None:intergenic
ACATATTAATGGATTAGCAG+TGG 0.658551 6:-109351907 MsG0680035612.01.T01:CDS
TCAATTGAGCCAGCTGATGG+TGG 0.665740 6:-109351816 MsG0680035612.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATGCAAGAAGCACATATTAA+TGG - Chr6:109351854-109351873 MsG0680035612.01.T01:CDS 30.0%
!! ACATATTAATGGATTAGCAG+TGG - Chr6:109351865-109351884 MsG0680035612.01.T01:CDS 30.0%
GCAATATCAACTGTCATTCC+AGG + Chr6:109351824-109351843 None:intergenic 40.0%
TGCATGCAATTGAGAGGAAA+TGG - Chr6:109351923-109351942 MsG0680035612.01.T01:CDS 40.0%
TTCCTCTCAATTGCATGCAA+TGG + Chr6:109351922-109351941 None:intergenic 40.0%
AGTTCAATTGAGCCAGCTGA+TGG - Chr6:109351953-109351972 MsG0680035612.01.T01:CDS 45.0%
GACCATTGCATGCAATTGAG+AGG - Chr6:109351917-109351936 MsG0680035612.01.T01:CDS 45.0%
TCAATTGAGCCAGCTGATGG+TGG - Chr6:109351956-109351975 MsG0680035612.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 109351809 109351985 109351809 ID=MsG0680035612.01;Name=MsG0680035612.01
Chr6 mRNA 109351809 109351985 109351809 ID=MsG0680035612.01.T01;Parent=MsG0680035612.01;Name=MsG0680035612.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|58
Chr6 exon 109351809 109351985 109351809 ID=MsG0680035612.01.T01:exon:351;Parent=MsG0680035612.01.T01
Chr6 CDS 109351809 109351985 109351809 ID=MsG0680035612.01.T01:cds;Parent=MsG0680035612.01.T01
Gene Sequence

>MsG0680035612.01.T01

ATGAAAGTGATACCTGGAATGACAGTTGATATTGCAGTTAACGTCATGCAAGAAGCACATATTAATGGATTAGCAGTGGTCATTATTTGTGCTCAAGTTGATGCTGAAGACCATTGCATGCAATTGAGAGGAAATGGTCTTTTAAGTTCAATTGAGCCAGCTGATGGTGGTTGTTGA

Protein sequence

>MsG0680035612.01.T01

MKVIPGMTVDIAVNVMQEAHINGLAVVIICAQVDAEDHCMQLRGNGLLSSIEPADGGC*