AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024695.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024695.01.T01 MTR_5g014600 96.703 91 3 0 2 92 121 211 1.94e-56 177
MsG0580024695.01.T01 MTR_4g097950 59.551 89 29 2 6 87 39 127 1.38e-26 98.6
MsG0580024695.01.T01 MTR_5g014520 59.302 86 30 1 12 92 134 219 5.73e-25 95.9
MsG0580024695.01.T01 MTR_4g092700 65.714 70 23 1 29 97 157 226 6.63e-25 95.5
MsG0580024695.01.T01 MTR_2g104550 65.333 75 25 1 16 90 125 198 7.90e-25 95.1
MsG0580024695.01.T01 MTR_5g014560 55.172 87 33 1 13 93 108 194 4.01e-24 93.2
MsG0580024695.01.T01 MTR_2g010450 70.492 61 18 0 28 88 175 235 5.15e-24 93.6
MsG0580024695.01.T01 MTR_4g097940 56.818 88 31 2 1 85 127 210 6.33e-24 92.8
MsG0580024695.01.T01 MTR_0246s0020 54.651 86 36 1 10 92 175 260 1.26e-23 92.8
MsG0580024695.01.T01 MTR_0246s0020 54.651 86 36 1 10 92 175 260 1.35e-23 92.4
MsG0580024695.01.T01 MTR_4g097920 56.322 87 31 2 1 84 112 194 2.13e-23 91.3
MsG0580024695.01.T01 MTR_4g097920 56.322 87 31 2 1 84 127 209 2.25e-23 91.3
MsG0580024695.01.T01 MTR_6g047550 52.564 78 37 0 15 92 158 235 2.73e-22 87.8
MsG0580024695.01.T01 MTR_5g014640 51.136 88 41 1 2 89 128 213 4.97e-22 87.8
MsG0580024695.01.T01 MTR_0011s0260 60.000 65 26 0 25 89 178 242 9.55e-22 87.4
MsG0580024695.01.T01 MTR_0011s0210 60.938 64 25 0 25 88 178 241 1.18e-21 87.0
MsG0580024695.01.T01 MTR_0246s0050 48.837 86 42 1 1 84 120 205 5.79e-21 84.7
MsG0580024695.01.T01 MTR_4g098035 48.837 86 42 1 1 84 141 226 8.31e-21 84.7
MsG0580024695.01.T01 MTR_0250s0040 51.316 76 37 0 17 92 75 150 1.02e-20 83.6
MsG0580024695.01.T01 MTR_6g047570 52.000 75 36 0 18 92 147 221 1.96e-20 83.6
MsG0580024695.01.T01 MTR_2g104490 59.722 72 28 1 16 87 124 194 4.46e-20 82.4
MsG0580024695.01.T01 MTR_2g104490 59.722 72 28 1 16 87 124 194 4.58e-20 82.0
MsG0580024695.01.T01 MTR_8g009020 63.333 60 22 0 29 88 142 201 3.32e-19 80.1
MsG0580024695.01.T01 MTR_2g104560 49.254 67 34 0 24 90 48 114 5.44e-19 78.2
MsG0580024695.01.T01 MTR_4g067010 47.436 78 38 1 14 88 128 205 7.03e-19 79.3
MsG0580024695.01.T01 MTR_4g066460 49.412 85 38 1 2 86 132 211 1.01e-18 79.0
MsG0580024695.01.T01 MTR_4g009540 51.220 82 40 0 6 87 128 209 1.20e-18 78.6
MsG0580024695.01.T01 MTR_4g009540 51.220 82 40 0 6 87 128 209 1.30e-18 78.6
MsG0580024695.01.T01 MTR_4g066380 47.619 84 39 1 2 85 130 208 1.41e-18 78.6
MsG0580024695.01.T01 MTR_7g096350 58.065 62 25 1 30 90 355 416 2.08e-18 79.0
MsG0580024695.01.T01 MTR_7g080780 48.684 76 38 1 13 87 134 209 2.26e-18 77.8
MsG0580024695.01.T01 MTR_7g080780 48.684 76 38 1 13 87 147 222 2.55e-18 77.8
MsG0580024695.01.T01 MTR_2g104590 56.716 67 28 1 18 84 58 123 8.25e-18 75.1
MsG0580024695.01.T01 MTR_4g009110 59.677 62 25 0 30 91 174 235 1.04e-17 76.3
MsG0580024695.01.T01 MTR_8g009010 60.000 60 24 0 29 88 133 192 1.71e-17 75.5
MsG0580024695.01.T01 MTR_7g117670 53.571 56 26 0 30 85 402 457 6.16e-16 71.6
MsG0580024695.01.T01 MTR_1g072320 50.820 61 30 0 28 88 468 528 6.73e-16 71.6
MsG0580024695.01.T01 MTR_8g027495 50.847 59 29 0 30 88 458 516 1.33e-15 70.9
MsG0580024695.01.T01 MTR_8g069740 53.731 67 30 1 18 84 101 166 3.66e-15 68.6
MsG0580024695.01.T01 MTR_8g067280 45.833 72 39 0 12 83 444 515 5.00e-15 69.3
MsG0580024695.01.T01 MTR_8g024790 37.805 82 48 1 7 88 271 349 2.87e-14 67.0
MsG0580024695.01.T01 MTR_5g030430 51.852 54 26 0 30 83 493 546 4.06e-14 66.6
MsG0580024695.01.T01 MTR_8g024770 36.842 76 45 1 7 82 259 331 8.83e-13 62.8
MsG0580024695.01.T01 MTR_7g110810 44.118 68 38 0 15 82 197 264 9.01e-13 62.8
MsG0580024695.01.T01 MTR_2g104500 41.975 81 35 2 9 88 94 163 1.78e-11 58.5
MsG0580024695.01.T01 MTR_1g112680 42.647 68 39 0 23 90 310 377 2.30e-11 58.9
MsG0580024695.01.T01 MTR_2g104530 50.847 59 28 1 31 88 106 164 2.49e-11 58.2
MsG0580024695.01.T01 MTR_2g104650 55.556 45 20 0 30 74 94 138 5.70e-11 57.4
MsG0580024695.01.T01 MTR_8g098275 31.868 91 57 1 2 92 378 463 6.47e-11 57.4
MsG0580024695.01.T01 MTR_8g098275 31.868 91 57 1 2 92 285 370 6.57e-11 57.4
MsG0580024695.01.T01 MTR_8g098275 31.868 91 57 1 2 92 221 306 7.86e-11 57.4
MsG0580024695.01.T01 MTR_1g019240 46.774 62 33 0 25 86 90 151 8.35e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024695.01.T01 AT4G37850 60.494 81 28 1 5 85 131 207 9.97e-25 95.1
MsG0580024695.01.T01 AT4G37850 60.494 81 28 1 5 85 131 207 9.97e-25 95.1
MsG0580024695.01.T01 AT2G22750 69.118 68 18 1 15 79 108 175 1.06e-24 94.7
MsG0580024695.01.T01 AT2G22750 69.118 68 18 1 15 79 108 175 1.13e-24 94.7
MsG0580024695.01.T01 AT2G22750 69.118 68 18 1 15 79 136 203 1.41e-24 94.7
MsG0580024695.01.T01 AT2G22750 69.118 68 18 1 15 79 136 203 1.58e-24 94.7
MsG0580024695.01.T01 AT2G22750 69.118 68 18 1 15 79 132 199 1.58e-24 94.7
MsG0580024695.01.T01 AT2G22760 63.333 60 22 0 28 87 117 176 2.58e-19 80.1
MsG0580024695.01.T01 AT2G22770 68.421 57 18 0 29 85 131 187 2.94e-19 80.5
MsG0580024695.01.T01 AT2G22760 63.333 60 22 0 28 87 117 176 3.33e-19 79.7
MsG0580024695.01.T01 AT2G46510 47.297 74 39 0 12 85 377 450 9.53e-18 77.0
MsG0580024695.01.T01 AT1G01260 38.272 81 50 0 8 88 411 491 2.58e-15 70.1
MsG0580024695.01.T01 AT1G01260 38.272 81 50 0 8 88 411 491 2.58e-15 70.1
MsG0580024695.01.T01 AT1G01260 38.272 81 50 0 8 88 411 491 2.58e-15 70.1
MsG0580024695.01.T01 AT4G16430 40.476 84 46 1 4 87 298 377 4.95e-15 69.3
MsG0580024695.01.T01 AT1G32640 41.379 87 46 1 12 93 434 520 2.27e-14 67.4
MsG0580024695.01.T01 AT4G17880 50.980 51 25 0 30 80 416 466 1.01e-13 65.5
MsG0580024695.01.T01 AT5G46760 50.000 54 27 0 30 83 415 468 1.13e-13 65.5
MsG0580024695.01.T01 AT4G09820 39.726 73 44 0 15 87 348 420 1.80e-12 62.0
MsG0580024695.01.T01 AT2G16910 45.000 60 33 0 31 90 315 374 2.39e-12 61.6
MsG0580024695.01.T01 AT4G00870 45.283 53 29 0 31 83 250 302 1.58e-11 59.3
MsG0580024695.01.T01 AT5G57150 42.647 68 38 1 17 83 41 108 1.91e-11 58.2
MsG0580024695.01.T01 AT4G36930 43.478 69 39 0 26 94 197 265 1.97e-11 58.9
MsG0580024695.01.T01 AT5G57150 42.647 68 38 1 17 83 41 108 2.05e-11 58.2
MsG0580024695.01.T01 AT5G57150 42.647 68 38 1 17 83 40 107 2.31e-11 58.2
MsG0580024695.01.T01 AT5G57150 42.029 69 39 1 16 83 40 108 2.42e-11 58.2
MsG0580024695.01.T01 AT5G57150 42.647 68 38 1 17 83 40 107 2.47e-11 57.8
MsG0580024695.01.T01 AT4G00050 37.805 82 47 1 5 86 196 273 4.56e-11 57.8
MsG0580024695.01.T01 AT1G09530 40.789 76 41 1 7 82 328 399 5.57e-11 57.8
MsG0580024695.01.T01 AT1G09530 40.789 76 41 1 7 82 328 399 5.57e-11 57.8
MsG0580024695.01.T01 AT1G09530 40.789 76 41 1 7 82 328 399 5.57e-11 57.8
MsG0580024695.01.T01 AT1G09530 40.789 76 41 1 7 82 328 399 5.57e-11 57.8
MsG0580024695.01.T01 AT1G09530 40.789 76 41 1 7 82 328 399 5.57e-11 57.8
MsG0580024695.01.T01 AT1G09530 40.789 76 41 1 7 82 328 399 5.57e-11 57.8
MsG0580024695.01.T01 AT4G00050 37.805 82 47 1 5 86 196 273 7.72e-11 57.4
MsG0580024695.01.T01 AT1G63650 36.986 73 45 1 11 83 387 458 9.91e-11 57.0
MsG0580024695.01.T01 AT1G63650 36.986 73 45 1 11 83 387 458 9.91e-11 57.0
MsG0580024695.01.T01 AT1G63650 36.986 73 45 1 11 83 387 458 9.91e-11 57.0

Find 24 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTGATCTTGAGCATTTC+TGG 0.145443 5:-8322359 None:intergenic
ATGGATAAGGCTTCTGTATT+AGG 0.248877 5:+8322556 MsG0580024695.01.T01:CDS
CTCTCTTGAATATTGCGTTT+TGG 0.303171 5:-8322316 None:intergenic
TGGATAAGGCTTCTGTATTA+GGG 0.314098 5:+8322557 MsG0580024695.01.T01:CDS
ACTCCTTCCAGACTTGAAAA+AGG 0.315242 5:+8322438 MsG0580024695.01.T01:CDS
GACACTCTTGCCTCAACTTC+AGG 0.353101 5:-8322786 None:intergenic
CTAGTAGTGTGCATTGCTAT+TGG 0.373914 5:+8322902 MsG0580024695.01.T01:three_prime_UTR
TCAGACACCTCATCAAATTC+TGG 0.375463 5:+8322709 MsG0580024695.01.T01:three_prime_UTR
AGATTTGTACGTTGTTCCTT+TGG 0.377524 5:-8322639 None:intergenic
AGATGAGAGTCTCTATCTCT+TGG 0.448727 5:-8322872 None:intergenic
CATTTGGATGTAGATGGATA+AGG 0.492545 5:+8322543 MsG0580024695.01.T01:intron
ATATCATACCTCAGCAATGA+TGG 0.498759 5:-8322946 None:intergenic
ACCTCATCAAATTCTGGCTA+TGG 0.514888 5:+8322715 MsG0580024695.01.T01:three_prime_UTR
TTTCTTGAAGCTGTTTCACA+TGG 0.520049 5:-8322591 None:intergenic
GAAGTTCAGCCTAAGCGTGC+AGG 0.528653 5:+8323236 MsG0580024695.01.T01:three_prime_UTR
CACTCTTATGTTTCTCACAA+TGG 0.534111 5:-8322833 None:intergenic
TAGCTAGCTCCTGCACGCTT+AGG 0.538929 5:-8323245 None:intergenic
TAGGTCACTGCCTGAAGTTG+AGG 0.613765 5:+8322776 MsG0580024695.01.T01:three_prime_UTR
TATTGGAGGAAAAGAACCAA+AGG 0.643739 5:+8322623 MsG0580024695.01.T01:CDS
ACCATAGCCAGAATTTGATG+AGG 0.651808 5:-8322716 None:intergenic
ACTCACACCTCAAAACAAGT+AGG 0.653322 5:+8322757 MsG0580024695.01.T01:three_prime_UTR
GGATAAGGCTTCTGTATTAG+GGG 0.656232 5:+8322558 MsG0580024695.01.T01:CDS
AGGAACAACGTACAAATCTG+TGG 0.661305 5:+8322643 MsG0580024695.01.T01:CDS
TGACATAACCATCATTGCTG+AGG 0.668356 5:+8322938 MsG0580024695.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATGATACTAATTAA+TGG + Chr5:8322508-8322527 MsG0580024695.01.T01:intron 10.0%
!! TAAATAACACTTATTTAAAA+TGG + Chr5:8323091-8323110 MsG0580024695.01.T01:intron 10.0%
!!! TGATTTTTTAACATCAAAAT+AGG + Chr5:8323130-8323149 MsG0580024695.01.T01:intron 15.0%
!!! ATAACACTTATTTAAAATGG+AGG + Chr5:8323094-8323113 MsG0580024695.01.T01:intron 20.0%
!!! CACTTATATTTTTAAAACAG+TGG - Chr5:8322981-8323000 None:intergenic 20.0%
!!! TAACACTTATTTAAAATGGA+GGG + Chr5:8323095-8323114 MsG0580024695.01.T01:intron 20.0%
! ATGGTTACTCATTAATCATT+TGG + Chr5:8322527-8322546 MsG0580024695.01.T01:intron 25.0%
! ATTAATCATTTGGATGTAGA+TGG + Chr5:8322537-8322556 MsG0580024695.01.T01:intron 25.0%
! TCAAGAAAAAGTGAAACTAT+TGG + Chr5:8322606-8322625 MsG0580024695.01.T01:CDS 25.0%
! TCCTTAGAAAACCAAAAAAA+GGG + Chr5:8322292-8322311 MsG0580024695.01.T01:CDS 25.0%
AGAAAAAGTGAAACTATTGG+AGG + Chr5:8322609-8322628 MsG0580024695.01.T01:CDS 30.0%
GTCCTTAGAAAACCAAAAAA+AGG + Chr5:8322291-8322310 MsG0580024695.01.T01:CDS 30.0%
GTTGAATCAATGTTCAACAT+TGG - Chr5:8322219-8322238 None:intergenic 30.0%
! ATGATTAAAACCTTGCCTTT+TGG - Chr5:8322249-8322268 None:intergenic 30.0%
! ATGGTTATGTCAAGTTTTTG+TGG - Chr5:8322930-8322949 None:intergenic 30.0%
!! AAAAGGCAAGGTTTTAATCA+TGG + Chr5:8322248-8322267 MsG0580024695.01.T01:intron 30.0%
!! AAAGGCAAGGTTTTAATCAT+GGG + Chr5:8322249-8322268 MsG0580024695.01.T01:intron 30.0%
!! GATTCAACTTTGAAACCAAA+AGG + Chr5:8322231-8322250 MsG0580024695.01.T01:intron 30.0%
!! TACTTAGTTTGTCTATCTTC+TGG + Chr5:8323194-8323213 MsG0580024695.01.T01:intron 30.0%
!!! AATTGAACCTTTTTCAAGTC+TGG - Chr5:8322448-8322467 None:intergenic 30.0%
!!! TCCCTTTTTTTGGTTTTCTA+AGG - Chr5:8322296-8322315 None:intergenic 30.0%
AGATTTGTACGTTGTTCCTT+TGG - Chr5:8322642-8322661 None:intergenic 35.0%
ATATCATACCTCAGCAATGA+TGG - Chr5:8322949-8322968 None:intergenic 35.0%
ATGGATAAGGCTTCTGTATT+AGG + Chr5:8322556-8322575 MsG0580024695.01.T01:CDS 35.0%
CACTCTTATGTTTCTCACAA+TGG - Chr5:8322836-8322855 None:intergenic 35.0%
CATTTGGATGTAGATGGATA+AGG + Chr5:8322543-8322562 MsG0580024695.01.T01:intron 35.0%
GGGAAATCTTTAACCTCAAA+GGG + Chr5:8322269-8322288 MsG0580024695.01.T01:intron 35.0%
TATGTGATCTTGAGCATTTC+TGG - Chr5:8322362-8322381 None:intergenic 35.0%
TATTGGAGGAAAAGAACCAA+AGG + Chr5:8322623-8322642 MsG0580024695.01.T01:CDS 35.0%
TGGATAAGGCTTCTGTATTA+GGG + Chr5:8322557-8322576 MsG0580024695.01.T01:CDS 35.0%
TGGGAAATCTTTAACCTCAA+AGG + Chr5:8322268-8322287 MsG0580024695.01.T01:intron 35.0%
! CTCTCTTGAATATTGCGTTT+TGG - Chr5:8322319-8322338 None:intergenic 35.0%
! TTTCTTGAAGCTGTTTCACA+TGG - Chr5:8322594-8322613 None:intergenic 35.0%
!! AACTTTGAAACCAAAAGGCA+AGG + Chr5:8322236-8322255 MsG0580024695.01.T01:intron 35.0%
ACCATAGCCAGAATTTGATG+AGG - Chr5:8322719-8322738 None:intergenic 40.0%
ACTCACACCTCAAAACAAGT+AGG + Chr5:8322757-8322776 MsG0580024695.01.T01:three_prime_UTR 40.0%
ACTCCTTCCAGACTTGAAAA+AGG + Chr5:8322438-8322457 MsG0580024695.01.T01:CDS 40.0%
AGATGAGAGTCTCTATCTCT+TGG - Chr5:8322875-8322894 None:intergenic 40.0%
AGGAACAACGTACAAATCTG+TGG + Chr5:8322643-8322662 MsG0580024695.01.T01:CDS 40.0%
CTAGTAGTGTGCATTGCTAT+TGG + Chr5:8322902-8322921 MsG0580024695.01.T01:three_prime_UTR 40.0%
GGATAAGGCTTCTGTATTAG+GGG + Chr5:8322558-8322577 MsG0580024695.01.T01:CDS 40.0%
TCAGACACCTCATCAAATTC+TGG + Chr5:8322709-8322728 MsG0580024695.01.T01:three_prime_UTR 40.0%
TCTTCTGGTACAAATGCAGA+TGG + Chr5:8323209-8323228 MsG0580024695.01.T01:intron 40.0%
TGACATAACCATCATTGCTG+AGG + Chr5:8322938-8322957 MsG0580024695.01.T01:three_prime_UTR 40.0%
! ACCTCATCAAATTCTGGCTA+TGG + Chr5:8322715-8322734 MsG0580024695.01.T01:three_prime_UTR 40.0%
!! TGCGTTTTGGTCCCTTTTTT+TGG - Chr5:8322306-8322325 None:intergenic 40.0%
!!! GAACCTTTTTCAAGTCTGGA+AGG - Chr5:8322444-8322463 None:intergenic 40.0%
!! GGTTTTCTAAGGACCCTTTG+AGG - Chr5:8322285-8322304 None:intergenic 45.0%
!!! GCAGTGACCTACTTGTTTTG+AGG - Chr5:8322767-8322786 None:intergenic 45.0%
GACACTCTTGCCTCAACTTC+AGG - Chr5:8322789-8322808 None:intergenic 50.0%
TAGGTCACTGCCTGAAGTTG+AGG + Chr5:8322776-8322795 MsG0580024695.01.T01:three_prime_UTR 50.0%
GAAGTTCAGCCTAAGCGTGC+AGG + Chr5:8323236-8323255 MsG0580024695.01.T01:three_prime_UTR 55.0%
TAGCTAGCTCCTGCACGCTT+AGG - Chr5:8323248-8323267 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 8322219 8323359 8322219 ID=MsG0580024695.01;Name=MsG0580024695.01
Chr5 mRNA 8322219 8323359 8322219 ID=MsG0580024695.01.T01;Parent=MsG0580024695.01;Name=MsG0580024695.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0.66|0.5|1|0|0|4|416|97
Chr5 exon 8322219 8322221 8322219 ID=MsG0580024695.01.T01:exon:26278;Parent=MsG0580024695.01.T01
Chr5 exon 8322289 8322459 8322289 ID=MsG0580024695.01.T01:exon:26279;Parent=MsG0580024695.01.T01
Chr5 exon 8322556 8322959 8322556 ID=MsG0580024695.01.T01:exon:26280;Parent=MsG0580024695.01.T01
Chr5 exon 8323228 8323359 8323228 ID=MsG0580024695.01.T01:exon:26281;Parent=MsG0580024695.01.T01
Chr5 CDS 8322219 8322221 8322219 ID=MsG0580024695.01.T01:cds;Parent=MsG0580024695.01.T01
Chr5 CDS 8322289 8322459 8322289 ID=MsG0580024695.01.T01:cds;Parent=MsG0580024695.01.T01
Chr5 CDS 8322556 8322675 8322556 ID=MsG0580024695.01.T01:cds;Parent=MsG0580024695.01.T01
Chr5 three_prime_UTR 8322676 8322959 8322676 ID=MsG0580024695.01.T01:three_prime_utr;Parent=MsG0580024695.01.T01
Chr5 three_prime_UTR 8323228 8323359 8323228 ID=MsG0580024695.01.T01:three_prime_utr;Parent=MsG0580024695.01.T01
Gene Sequence

>MsG0580024695.01.T01

ATGGGGTCCTTAGAAAACCAAAAAAAGGGACCAAAACGCAATATTCAAGAGAGCAAGAAGACTGATTCAGCTGCCAGAAATGCTCAAGATCACATACTTGCTGAGAGAAAGAGAAGAGAAAAAATTAGTCAAAAGTTCATAGCTCTTTCAGCACTCCTTCCAGACTTGAAAAAGATGGATAAGGCTTCTGTATTAGGGGATGCTATAAACCATGTGAAACAGCTTCAAGAAAAAGTGAAACTATTGGAGGAAAAGAACCAAAGGAACAACGTACAAATCTGTGGTATATGTTGA

Protein sequence

>MsG0580024695.01.T01

MGSLENQKKGPKRNIQESKKTDSAARNAQDHILAERKRREKISQKFIALSALLPDLKKMDKASVLGDAINHVKQLQEKVKLLEEKNQRNNVQICGIC*