AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041253.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041253.01.T01 MTR_7g107670 94.776 268 14 0 43 310 31 298 0.0 524
MsG0780041253.01.T01 MTR_3g113870 86.325 234 20 1 37 258 1 234 1.81e-143 404
MsG0780041253.01.T01 MTR_7g083870 55.124 283 122 2 28 309 22 300 5.02e-111 324
MsG0780041253.01.T01 MTR_4g052350 50.566 265 122 3 37 300 6 262 2.37e-85 258
MsG0780041253.01.T01 MTR_7g071680 44.853 272 136 2 43 300 27 298 4.95e-84 255
MsG0780041253.01.T01 MTR_4g052340 49.434 265 118 4 42 298 11 267 2.65e-81 247
MsG0780041253.01.T01 MTR_4g052320 49.612 258 122 3 44 300 7 257 1.86e-80 245
MsG0780041253.01.T01 MTR_4g052300 49.225 258 122 3 44 300 7 256 1.55e-77 238
MsG0780041253.01.T01 MTR_7g105460 48.425 254 125 2 44 296 12 260 2.13e-73 227
MsG0780041253.01.T01 MTR_7g110130 43.750 256 137 3 43 296 4 254 1.91e-70 219
MsG0780041253.01.T01 MTR_4g078630 44.788 259 136 5 43 297 17 272 5.29e-70 219
MsG0780041253.01.T01 MTR_2g090165 44.531 256 134 4 43 296 4 253 2.36e-69 216
MsG0780041253.01.T01 MTR_7g105580 44.922 256 117 3 43 297 6 238 2.94e-69 216
MsG0780041253.01.T01 MTR_3g020670 45.914 257 136 2 43 296 14 270 6.38e-68 213
MsG0780041253.01.T01 MTR_7g110140 43.609 266 142 4 44 307 5 264 7.14e-65 205
MsG0780041253.01.T01 MTR_6g023590 40.541 259 146 4 44 299 12 265 1.87e-62 199
MsG0780041253.01.T01 MTR_6g035395 42.529 261 131 5 43 302 3 245 2.98e-62 198
MsG0780041253.01.T01 MTR_6g023990 40.927 259 145 4 44 299 13 266 3.33e-61 196
MsG0780041253.01.T01 MTR_7g105590 52.128 188 89 1 43 229 6 193 5.27e-61 193
MsG0780041253.01.T01 MTR_6g023910 40.385 260 147 4 44 300 12 266 2.20e-60 194
MsG0780041253.01.T01 MTR_7g110160 42.188 256 122 4 43 296 4 235 3.03e-60 192
MsG0780041253.01.T01 MTR_6g023950 41.569 255 141 4 44 295 12 261 1.40e-59 191
MsG0780041253.01.T01 MTR_6g023610 39.231 260 148 5 44 299 12 265 6.15e-58 187
MsG0780041253.01.T01 MTR_6g023600 40.613 261 143 5 44 299 12 265 4.21e-56 182
MsG0780041253.01.T01 MTR_6g023600 39.847 261 139 6 44 299 12 259 5.14e-52 172
MsG0780041253.01.T01 MTR_7g105570 38.202 267 107 3 33 296 6 217 5.87e-51 168
MsG0780041253.01.T01 MTR_4g084800 52.632 133 62 1 43 174 11 143 1.67e-40 139
MsG0780041253.01.T01 MTR_3g085740 28.873 284 174 7 28 295 47 318 8.89e-30 115
MsG0780041253.01.T01 MTR_4g132850 28.462 260 167 7 43 297 5 250 1.75e-26 105
MsG0780041253.01.T01 MTR_1196s0010 28.626 262 160 8 42 295 271 513 1.50e-23 100
MsG0780041253.01.T01 MTR_3g068155 28.958 259 168 7 45 298 19 266 7.58e-23 95.9
MsG0780041253.01.T01 MTR_7g094510 27.969 261 166 8 45 295 6 254 1.51e-22 94.7
MsG0780041253.01.T01 MTR_3g068070 27.341 267 180 7 34 296 4 260 2.78e-21 91.7
MsG0780041253.01.T01 MTR_7g094520 25.969 258 175 5 45 295 11 259 4.50e-21 90.9
MsG0780041253.01.T01 MTR_3g068085 27.519 258 169 7 45 296 15 260 2.92e-20 89.0
MsG0780041253.01.T01 MTR_3g068065 26.848 257 174 6 45 297 17 263 3.34e-20 88.6
MsG0780041253.01.T01 MTR_4g064887 26.744 258 171 6 45 296 15 260 4.31e-19 85.5
MsG0780041253.01.T01 MTR_4g131740 28.947 266 159 10 44 294 39 289 4.70e-19 85.9
MsG0780041253.01.T01 MTR_3g068175 27.692 260 166 7 45 296 17 262 6.70e-19 85.1
MsG0780041253.01.T01 MTR_7g113650 26.316 266 165 8 44 294 39 288 1.19e-18 84.7
MsG0780041253.01.T01 MTR_8g442750 31.955 266 152 11 45 295 14 265 3.30e-18 83.6
MsG0780041253.01.T01 MTR_7g063330 28.958 259 169 6 45 297 15 264 4.77e-18 82.4
MsG0780041253.01.T01 MTR_4g097510 28.571 196 137 2 46 240 3 196 1.63e-17 81.3
MsG0780041253.01.T01 MTR_1g082760 26.136 264 168 7 44 294 39 288 2.20e-17 81.3
MsG0780041253.01.T01 MTR_3g068175 27.273 264 166 8 45 296 17 266 4.33e-17 80.1
MsG0780041253.01.T01 MTR_7g063270 29.057 265 161 7 45 297 16 265 1.20e-16 78.6
MsG0780041253.01.T01 MTR_6g023940 27.041 196 82 3 44 238 12 147 1.25e-16 76.3
MsG0780041253.01.T01 MTR_1g068955 28.358 268 153 7 45 293 14 261 2.38e-16 78.2
MsG0780041253.01.T01 MTR_7g063260 28.571 259 171 6 45 297 16 266 3.80e-16 77.4
MsG0780041253.01.T01 MTR_7g080410 30.928 194 124 5 45 230 38 229 4.81e-16 77.8
MsG0780041253.01.T01 MTR_7g080410 30.928 194 124 5 45 230 38 229 7.57e-16 77.0
MsG0780041253.01.T01 MTR_8g096580 29.557 203 129 7 45 239 45 241 1.64e-15 76.3
MsG0780041253.01.T01 MTR_7g063390 28.682 258 170 7 45 296 16 265 2.59e-15 74.7
MsG0780041253.01.T01 MTR_4g064883 25.000 256 178 7 45 296 14 259 2.68e-15 74.7
MsG0780041253.01.T01 MTR_1g051165 25.556 270 175 8 43 296 12 271 3.30e-15 74.7
MsG0780041253.01.T01 MTR_4g086400 26.619 278 158 11 50 308 9 259 4.30e-15 76.3
MsG0780041253.01.T01 MTR_7g063320 28.839 267 156 8 45 295 15 263 4.72e-15 73.9
MsG0780041253.01.T01 MTR_4g058810 28.409 264 166 9 45 296 11 263 1.97e-14 72.4
MsG0780041253.01.T01 MTR_3g087850 28.962 183 121 4 49 228 30 206 4.34e-14 71.6
MsG0780041253.01.T01 MTR_4g058840 27.969 261 171 8 45 296 11 263 1.02e-13 70.5
MsG0780041253.01.T01 MTR_4g058820 27.969 261 171 8 45 296 11 263 1.02e-13 70.5
MsG0780041253.01.T01 MTR_4g086410 29.703 202 122 5 50 237 9 204 2.10e-13 70.9
MsG0780041253.01.T01 MTR_4g064887 27.835 194 130 4 45 232 15 204 2.79e-13 68.6
MsG0780041253.01.T01 MTR_7g105560 56.897 58 21 1 43 100 11 64 1.50e-12 64.7
MsG0780041253.01.T01 MTR_4g064883 25.907 193 137 4 45 233 14 204 3.11e-12 65.1
MsG0780041253.01.T01 MTR_8g044240 26.667 195 133 4 51 235 75 269 5.43e-12 65.9
MsG0780041253.01.T01 MTR_3g087860 26.627 169 116 3 62 228 29 191 8.26e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041253.01.T01 AT4G03140 72.659 267 73 0 43 309 76 342 1.06e-143 409
MsG0780041253.01.T01 AT3G26770 56.604 265 114 1 43 306 39 303 3.65e-110 322
MsG0780041253.01.T01 AT3G26760 53.759 266 120 2 43 305 34 299 1.92e-105 310
MsG0780041253.01.T01 AT3G51680 46.667 270 132 4 43 300 30 299 1.15e-77 239
MsG0780041253.01.T01 AT2G47130 47.104 259 126 6 44 299 5 255 6.41e-76 233
MsG0780041253.01.T01 AT2G47120 45.385 260 132 6 42 299 4 255 2.38e-75 232
MsG0780041253.01.T01 AT2G47120 45.349 258 131 6 44 299 5 254 7.37e-75 230
MsG0780041253.01.T01 AT3G29250 44.251 287 143 6 24 299 14 294 2.38e-74 231
MsG0780041253.01.T01 AT3G29250 46.899 258 129 4 44 299 5 256 2.71e-74 229
MsG0780041253.01.T01 AT3G29250 44.406 286 142 6 24 299 14 292 6.69e-74 229
MsG0780041253.01.T01 AT3G29260 44.231 260 134 5 43 299 4 255 3.27e-71 221
MsG0780041253.01.T01 AT2G47140 42.085 259 142 5 43 299 4 256 2.50e-67 211
MsG0780041253.01.T01 AT1G52340 46.545 275 121 7 43 299 16 282 2.50e-66 209
MsG0780041253.01.T01 AT3G42960 43.077 260 140 6 43 297 6 262 5.18e-61 196
MsG0780041253.01.T01 AT2G47150 33.594 256 111 8 45 299 1 198 8.70e-32 117
MsG0780041253.01.T01 AT5G18210 30.303 264 159 9 45 295 8 259 1.19e-23 97.8
MsG0780041253.01.T01 AT1G07450 28.794 257 166 6 45 296 8 252 3.34e-23 96.7
MsG0780041253.01.T01 AT4G05530 30.000 260 163 7 43 297 7 252 7.54e-23 95.5
MsG0780041253.01.T01 AT1G24360 28.571 259 168 7 42 295 71 317 1.19e-22 96.3
MsG0780041253.01.T01 AT2G29310 28.516 256 169 5 45 296 7 252 1.23e-22 95.1
MsG0780041253.01.T01 AT5G06060 27.344 256 172 5 45 296 9 254 2.90e-22 94.4
MsG0780041253.01.T01 AT2G29340 27.734 256 171 5 45 296 7 252 5.58e-22 93.2
MsG0780041253.01.T01 AT2G29340 27.734 256 171 5 45 296 7 252 8.20e-22 94.0
MsG0780041253.01.T01 AT1G63380 29.412 289 175 11 19 296 2 272 1.59e-21 92.4
MsG0780041253.01.T01 AT1G63380 29.924 264 165 9 44 296 16 270 1.73e-21 92.4
MsG0780041253.01.T01 AT1G63380 29.924 264 165 9 44 296 14 268 1.75e-21 92.4
MsG0780041253.01.T01 AT1G63380 29.924 264 165 9 44 296 21 275 2.00e-21 92.4
MsG0780041253.01.T01 AT2G30670 28.405 257 168 7 45 296 7 252 5.74e-21 90.5
MsG0780041253.01.T01 AT2G29330 27.344 256 172 6 45 296 7 252 2.17e-20 89.0
MsG0780041253.01.T01 AT1G54870 27.925 265 162 8 44 294 81 330 4.16e-20 89.4
MsG0780041253.01.T01 AT1G62610 28.720 289 177 11 19 296 2 272 4.27e-20 88.6
MsG0780041253.01.T01 AT1G62610 29.167 264 167 9 44 296 13 267 4.46e-20 88.6
MsG0780041253.01.T01 AT1G62610 29.167 264 167 9 44 296 12 266 4.48e-20 88.6
MsG0780041253.01.T01 AT1G54870 27.925 265 162 8 44 294 83 332 4.65e-20 89.4
MsG0780041253.01.T01 AT1G62610 29.167 264 167 9 44 296 14 268 5.10e-20 88.2
MsG0780041253.01.T01 AT2G29360 29.924 264 159 10 50 301 21 270 1.17e-19 87.0
MsG0780041253.01.T01 AT5G18210 29.924 264 147 10 45 295 8 246 2.21e-19 86.7
MsG0780041253.01.T01 AT3G55290 30.075 266 161 9 44 296 16 269 2.25e-19 86.7
MsG0780041253.01.T01 AT3G55290 30.075 266 161 9 44 296 17 270 2.58e-19 86.3
MsG0780041253.01.T01 AT2G29300 25.475 263 173 7 45 298 7 255 3.33e-19 86.3
MsG0780041253.01.T01 AT2G29320 27.170 265 168 7 42 296 10 259 4.38e-19 85.5
MsG0780041253.01.T01 AT2G29370 29.845 258 163 9 45 296 16 261 5.27e-19 85.5
MsG0780041253.01.T01 AT2G29300 25.475 263 173 7 45 298 7 255 5.38e-19 85.1
MsG0780041253.01.T01 AT2G29350 28.090 267 169 7 45 301 15 268 5.74e-19 85.1
MsG0780041253.01.T01 AT3G55310 30.827 266 159 9 44 296 16 269 7.60e-19 85.1
MsG0780041253.01.T01 AT3G03980 27.820 266 167 7 45 295 14 269 7.94e-19 84.7
MsG0780041253.01.T01 AT2G17845 28.947 266 163 8 44 294 46 300 9.97e-19 85.1
MsG0780041253.01.T01 AT1G07440 27.734 256 170 6 45 296 12 256 1.28e-18 84.3
MsG0780041253.01.T01 AT3G46170 29.389 262 168 7 44 296 25 278 1.49e-18 84.3
MsG0780041253.01.T01 AT4G13180 28.462 260 166 7 45 295 13 261 1.73e-18 84.0
MsG0780041253.01.T01 AT2G29150 27.692 260 161 8 45 294 16 258 6.88e-18 82.0
MsG0780041253.01.T01 AT3G04000 26.937 271 162 6 45 294 14 269 1.40e-17 81.3
MsG0780041253.01.T01 AT1G49670 31.633 196 117 5 50 230 9 202 2.50e-17 82.8
MsG0780041253.01.T01 AT2G29260 26.718 262 176 7 50 307 73 322 2.65e-17 81.3
MsG0780041253.01.T01 AT1G49670 31.633 196 117 5 50 230 9 202 2.74e-17 82.8
MsG0780041253.01.T01 AT3G05260 26.616 263 169 7 44 295 36 285 1.08e-16 79.3
MsG0780041253.01.T01 AT2G29290 26.562 256 174 5 45 296 7 252 1.14e-16 78.6
MsG0780041253.01.T01 AT2G29340 28.205 195 134 3 45 235 7 199 2.06e-16 76.6
MsG0780041253.01.T01 AT2G29340 28.205 195 134 3 45 235 7 199 2.06e-16 76.6
MsG0780041253.01.T01 AT3G12800 29.562 274 167 8 45 304 10 271 2.70e-16 78.2
MsG0780041253.01.T01 AT2G29310 28.718 195 133 3 45 235 7 199 5.60e-16 75.5
MsG0780041253.01.T01 AT1G10310 30.526 190 128 3 48 235 18 205 5.82e-16 76.3
MsG0780041253.01.T01 AT2G29310 26.522 230 155 5 71 296 31 250 6.41e-16 76.6
MsG0780041253.01.T01 AT3G47350 27.854 219 146 6 32 241 26 241 4.45e-15 74.7
MsG0780041253.01.T01 AT3G47350 27.854 219 146 6 32 241 26 241 5.00e-15 74.7
MsG0780041253.01.T01 AT2G29350 29.101 189 124 4 50 232 20 204 1.75e-14 72.0
MsG0780041253.01.T01 AT2G29350 26.754 228 149 6 79 301 16 230 4.68e-14 70.9
MsG0780041253.01.T01 AT5G04900 27.320 194 132 3 51 235 83 276 2.49e-13 70.1
MsG0780041253.01.T01 AT2G29320 27.835 194 133 4 42 230 10 201 2.82e-13 68.2
MsG0780041253.01.T01 AT5G65205 29.032 186 123 4 46 228 7 186 3.17e-13 69.3
MsG0780041253.01.T01 AT3G47360 27.027 222 142 7 32 243 26 237 1.17e-11 64.7
MsG0780041253.01.T01 AT2G29290 24.545 220 153 5 78 296 28 235 3.06e-11 62.8
MsG0780041253.01.T01 AT3G03330 27.551 196 129 4 45 230 41 233 3.20e-11 63.5

Find 70 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCACAATTAGCTCCTTTC+AGG 0.179729 7:-88829594 None:intergenic
GACAAGGTAGCACTGATAAC+TGG 0.262621 7:+88828959 MsG0780041253.01.T01:CDS
GTATCCAAGTTTGCGGTTAT+AGG 0.264110 7:+88829409 MsG0780041253.01.T01:CDS
GTATATCAGCGGGCATAATT+TGG 0.276919 7:+88829666 MsG0780041253.01.T01:CDS
TTTGTTATTGCAAGTTATCT+TGG 0.318208 7:-88829925 None:intergenic
GTGTGAGATCTAATTCATTT+TGG 0.324717 7:+88828262 MsG0780041253.01.T01:CDS
GCAACAAAATTCATCAATAA+TGG 0.333166 7:+88829010 MsG0780041253.01.T01:CDS
AATAAATGTTCGCGGCGTTA+TGG 0.349280 7:+88829276 MsG0780041253.01.T01:CDS
TAGCTCATTATATAACTATA+TGG 0.371753 7:+88829875 MsG0780041253.01.T01:three_prime_UTR
GTAATGATCCCGCGTGGAAC+TGG 0.374917 7:+88829322 MsG0780041253.01.T01:CDS
CTATGCACTGCCAGCGTCAC+TGG 0.405254 7:+88829352 MsG0780041253.01.T01:CDS
TTTCTTGGCCAAGTTGTTGT+TGG 0.415820 7:-88829057 None:intergenic
TCTTTCTAATGCACTTGATC+TGG 0.425144 7:-88829736 None:intergenic
AATGTTCGCGGCGTTATGGC+TGG 0.433632 7:+88829280 MsG0780041253.01.T01:CDS
AATGCCTATAACCGCAAACT+TGG 0.438642 7:-88829413 None:intergenic
CCATGGCTTCAGAGCTATCC+AGG 0.442092 7:+88829443 MsG0780041253.01.T01:CDS
TCATGTTAAGAATTTGTTTG+AGG 0.443755 7:+88828292 MsG0780041253.01.T01:CDS
ATTGACTCGGATTCCATGCC+TGG 0.445621 7:-88829461 None:intergenic
CAAGTTATGAAGGTAGCATT+AGG 0.456185 7:-88829097 None:intergenic
GATATCATGTATAACAATGC+AGG 0.475659 7:+88829187 MsG0780041253.01.T01:CDS
ATACTCTGTATCCAAGTTTG+CGG 0.478404 7:+88829402 MsG0780041253.01.T01:CDS
CATAATTTGGTTGTGGATGG+AGG 0.480867 7:+88829679 MsG0780041253.01.T01:CDS
GTAGAAATTGTTCGTAATGT+TGG 0.482699 7:+88829568 MsG0780041253.01.T01:CDS
TATAGGCATTGTTAAATCCA+TGG 0.486525 7:+88829426 MsG0780041253.01.T01:CDS
AGATTGAGGTCAACAACGCT+TGG 0.496330 7:-88829229 None:intergenic
GCCAGCGTCACTGGTGTGAT+TGG 0.498683 7:+88829361 MsG0780041253.01.T01:CDS
TAATGCACTTGATCTGGTGC+AGG 0.501427 7:-88829730 None:intergenic
TCCATTTCGCTCATGACAAA+AGG 0.502082 7:-88829511 None:intergenic
CAGCGGGCATAATTTGGTTG+TGG 0.504711 7:+88829672 MsG0780041253.01.T01:CDS
ACAAGGTAGCACTGATAACT+GGG 0.508049 7:+88828960 MsG0780041253.01.T01:CDS
TTAAAGCTTGTTTAAAGGGG+TGG 0.510748 7:+88829776 MsG0780041253.01.T01:three_prime_UTR
CAAGAAACTGCAAAAGAACT+AGG 0.513734 7:+88829073 MsG0780041253.01.T01:CDS
AAGTTGTTTGTATTCAGAAA+CGG 0.514499 7:-88829164 None:intergenic
ACCTTGCATCTAATGATGCT+AGG 0.517372 7:+88829644 MsG0780041253.01.T01:CDS
CACTGGTGTGATTGGAGGGA+TGG 0.517491 7:+88829369 MsG0780041253.01.T01:CDS
GACGCTGGCAGTGCATAGAA+TGG 0.522764 7:-88829347 None:intergenic
CCTGGATAGCTCTGAAGCCA+TGG 0.523976 7:-88829443 None:intergenic
TGTCAAACGATTCTAGATTG+AGG 0.524531 7:-88829243 None:intergenic
TGTGACGTCGCAAGTTATGA+AGG 0.529384 7:-88829107 None:intergenic
AGCGTCACTGGTGTGATTGG+AGG 0.541442 7:+88829364 MsG0780041253.01.T01:CDS
GCTGATATCCAACAACAACT+TGG 0.550507 7:+88829049 MsG0780041253.01.T01:CDS
TTGAGGTCAACAACGCTTGG+AGG 0.555513 7:-88829226 None:intergenic
GGGCATAATTTGGTTGTGGA+TGG 0.562134 7:+88829676 MsG0780041253.01.T01:CDS
ACCTAGCATCATTAGATGCA+AGG 0.564196 7:-88829645 None:intergenic
TCCAATCACACCAGTGACGC+TGG 0.565675 7:-88829362 None:intergenic
TAATAATAAATATATAACGT+TGG 0.566848 7:+88829831 MsG0780041253.01.T01:three_prime_UTR
AAAGGAGTAGGAATAGCAAA+AGG 0.575525 7:-88829493 None:intergenic
TGCAGCATTAGCGATATCAT+CGG 0.580679 7:-88829617 None:intergenic
GCAGCATTAGCGATATCATC+GGG 0.582609 7:-88829616 None:intergenic
GTCATGGAAATAAATGTTCG+CGG 0.584404 7:+88829268 MsG0780041253.01.T01:CDS
CGTAATGTTGGTGTCCTGAA+AGG 0.587683 7:+88829580 MsG0780041253.01.T01:CDS
GCGTCACTGGTGTGATTGGA+GGG 0.588989 7:+88829365 MsG0780041253.01.T01:CDS
ATGCATGGACTGTGGTGTGA+TGG 0.590946 7:-88828069 None:intergenic
ATATCATGTATAACAATGCA+GGG 0.592185 7:+88829188 MsG0780041253.01.T01:CDS
CAAATTATTCAGAACTTGTG+CGG 0.596886 7:+88829944 MsG0780041253.01.T01:three_prime_UTR
TCGCTCATGACAAAAGGAGT+AGG 0.599202 7:-88829505 None:intergenic
AGAATCGTTTGACAAAGTCA+TGG 0.604965 7:+88829252 MsG0780041253.01.T01:CDS
GCTTCAGAGCTATCCAGGCA+TGG 0.629348 7:+88829448 MsG0780041253.01.T01:CDS
ATGAATTAGATCTCACACAC+TGG 0.629530 7:-88828257 None:intergenic
ATGATGCTAGGTATATCAGC+GGG 0.660569 7:+88829656 MsG0780041253.01.T01:CDS
GTCCAGGAAGCTACAAGACA+AGG 0.662037 7:+88828943 MsG0780041253.01.T01:intron
AAGGCGATATACAATTGACT+CGG 0.673213 7:-88829474 None:intergenic
AGTCGCTGCGAATCAAGATG+TGG 0.674277 7:-88829544 None:intergenic
GAATGGATCCAGTTCCACGC+GGG 0.678631 7:-88829330 None:intergenic
GGGATAAGATGCATGGACTG+TGG 0.688907 7:-88828077 None:intergenic
AATGATGCTAGGTATATCAG+CGG 0.703024 7:+88829655 MsG0780041253.01.T01:CDS
AAGGTAGCACTGATAACTGG+GGG 0.709904 7:+88828962 MsG0780041253.01.T01:CDS
CAAGGTAGCACTGATAACTG+GGG 0.710678 7:+88828961 MsG0780041253.01.T01:CDS
GCGCGTGTAATGATCCCGCG+TGG 0.726381 7:+88829316 MsG0780041253.01.T01:CDS
AGAATGGATCCAGTTCCACG+CGG 0.782409 7:-88829331 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAGTAAATATTATCAAA+AGG + Chr7:88828872-88828891 MsG0780041253.01.T01:intron 10.0%
!! TAATAATAAATATATAACGT+TGG + Chr7:88829831-88829850 MsG0780041253.01.T01:three_prime_UTR 10.0%
!!! TATATTTTATTCTATAACTT+TGG + Chr7:88828430-88828449 MsG0780041253.01.T01:intron 10.0%
!!! AATTTTTAAAGCTTGTTTAA+AGG + Chr7:88829771-88829790 MsG0780041253.01.T01:three_prime_UTR 15.0%
!!! ATTTTTAAAGCTTGTTTAAA+GGG + Chr7:88829772-88829791 MsG0780041253.01.T01:three_prime_UTR 15.0%
!!! TAATTTTTATTTGTCTCATA+GGG + Chr7:88828215-88828234 MsG0780041253.01.T01:intron 15.0%
!!! TTTTCTATTTATCTATAAGA+TGG + Chr7:88828656-88828675 MsG0780041253.01.T01:intron 15.0%
!! TAGCTCATTATATAACTATA+TGG + Chr7:88829875-88829894 MsG0780041253.01.T01:three_prime_UTR 20.0%
!!! CTAATTTTTATTTGTCTCAT+AGG + Chr7:88828214-88828233 MsG0780041253.01.T01:intron 20.0%
!!! TATAATTTGCATATATTCGT+TGG + Chr7:88828122-88828141 MsG0780041253.01.T01:intron 20.0%
!!! TTTCACATCTTTTAAGAATT+TGG + Chr7:88829702-88829721 MsG0780041253.01.T01:CDS 20.0%
!!! TTTTTAAAGCTTGTTTAAAG+GGG + Chr7:88829773-88829792 MsG0780041253.01.T01:three_prime_UTR 20.0%
! AAATTATTATCTACTCAGAC+TGG + Chr7:88828581-88828600 MsG0780041253.01.T01:intron 25.0%
! AAGTTGTTTGTATTCAGAAA+CGG - Chr7:88829167-88829186 None:intergenic 25.0%
! ATATCATGTATAACAATGCA+GGG + Chr7:88829188-88829207 MsG0780041253.01.T01:CDS 25.0%
! CTGAAAACTTATTTACAACT+TGG - Chr7:88828483-88828502 None:intergenic 25.0%
! GCAACAAAATTCATCAATAA+TGG + Chr7:88829010-88829029 MsG0780041253.01.T01:CDS 25.0%
! TCTTATCCCAAAAAATATCA+CGG + Chr7:88828091-88828110 MsG0780041253.01.T01:CDS 25.0%
!! TCATGTTAAGAATTTGTTTG+AGG + Chr7:88828292-88828311 MsG0780041253.01.T01:CDS 25.0%
!!! TGACTAAGAGTATTATATCA+TGG - Chr7:88828785-88828804 None:intergenic 25.0%
!!! TTATAGAAAAAGTACTGCAA+GGG - Chr7:88828164-88828183 None:intergenic 25.0%
!!! TTTGTTATTGCAAGTTATCT+TGG - Chr7:88829928-88829947 None:intergenic 25.0%
CAAATTATTCAGAACTTGTG+CGG + Chr7:88829944-88829963 MsG0780041253.01.T01:three_prime_UTR 30.0%
CTTATCCCAAAAAATATCAC+GGG + Chr7:88828092-88828111 MsG0780041253.01.T01:CDS 30.0%
GAAATAATCAGAGAACATGA+AGG - Chr7:88828461-88828480 None:intergenic 30.0%
GATATCATGTATAACAATGC+AGG + Chr7:88829187-88829206 MsG0780041253.01.T01:CDS 30.0%
GTAGAAATTGTTCGTAATGT+TGG + Chr7:88829568-88829587 MsG0780041253.01.T01:CDS 30.0%
TTATCCCAAAAAATATCACG+GGG + Chr7:88828093-88828112 MsG0780041253.01.T01:CDS 30.0%
! GTGTGAGATCTAATTCATTT+TGG + Chr7:88828262-88828281 MsG0780041253.01.T01:CDS 30.0%
! GTTAAGAATTTGTTTGAGGT+TGG + Chr7:88828296-88828315 MsG0780041253.01.T01:intron 30.0%
! TATAGGCATTGTTAAATCCA+TGG + Chr7:88829426-88829445 MsG0780041253.01.T01:CDS 30.0%
!! GTTATAGAAAAAGTACTGCA+AGG - Chr7:88828165-88828184 None:intergenic 30.0%
!!! ATTTTTTGGGATAAGATGCA+TGG - Chr7:88828087-88828106 None:intergenic 30.0%
AAAGGAGTAGGAATAGCAAA+AGG - Chr7:88829496-88829515 None:intergenic 35.0%
AAGGCGATATACAATTGACT+CGG - Chr7:88829477-88829496 None:intergenic 35.0%
AATGATGCTAGGTATATCAG+CGG + Chr7:88829655-88829674 MsG0780041253.01.T01:CDS 35.0%
AGAATCGTTTGACAAAGTCA+TGG + Chr7:88829252-88829271 MsG0780041253.01.T01:CDS 35.0%
AGAGAAACTGAAGGATTGTT+TGG - Chr7:88828616-88828635 None:intergenic 35.0%
ATAATCAGAGAACATGAAGG+AGG - Chr7:88828458-88828477 None:intergenic 35.0%
ATACTCTGTATCCAAGTTTG+CGG + Chr7:88829402-88829421 MsG0780041253.01.T01:CDS 35.0%
ATGAATTAGATCTCACACAC+TGG - Chr7:88828260-88828279 None:intergenic 35.0%
CAAGAAACTGCAAAAGAACT+AGG + Chr7:88829073-88829092 MsG0780041253.01.T01:CDS 35.0%
CAAGTTATGAAGGTAGCATT+AGG - Chr7:88829100-88829119 None:intergenic 35.0%
CATCATTTGATCATCCTCTA+TGG - Chr7:88828846-88828865 None:intergenic 35.0%
GTCATGGAAATAAATGTTCG+CGG + Chr7:88829268-88829287 MsG0780041253.01.T01:CDS 35.0%
TATTATCTACTCAGACTGGA+AGG + Chr7:88828585-88828604 MsG0780041253.01.T01:intron 35.0%
TCTTTCTAATGCACTTGATC+TGG - Chr7:88829739-88829758 None:intergenic 35.0%
TGTCAAACGATTCTAGATTG+AGG - Chr7:88829246-88829265 None:intergenic 35.0%
! TCATCCTCTATGGTTTCTTT+TGG - Chr7:88828836-88828855 None:intergenic 35.0%
! TTAAAGCTTGTTTAAAGGGG+TGG + Chr7:88829776-88829795 MsG0780041253.01.T01:three_prime_UTR 35.0%
!! AAAGTACTGCAAGGGTAATT+TGG - Chr7:88828156-88828175 None:intergenic 35.0%
!! CTAGTTCTTTTGCAGTTTCT+TGG - Chr7:88829075-88829094 None:intergenic 35.0%
!!! CATACCCCGTGATATTTTTT+GGG - Chr7:88828100-88828119 None:intergenic 35.0%
AATAAATGTTCGCGGCGTTA+TGG + Chr7:88829276-88829295 MsG0780041253.01.T01:CDS 40.0%
AATGCCTATAACCGCAAACT+TGG - Chr7:88829416-88829435 None:intergenic 40.0%
ACCTAGCATCATTAGATGCA+AGG - Chr7:88829648-88829667 None:intergenic 40.0%
ACCTTGCATCTAATGATGCT+AGG + Chr7:88829644-88829663 MsG0780041253.01.T01:CDS 40.0%
ATGATGCTAGGTATATCAGC+GGG + Chr7:88829656-88829675 MsG0780041253.01.T01:CDS 40.0%
CATAATTTGGTTGTGGATGG+AGG + Chr7:88829679-88829698 MsG0780041253.01.T01:CDS 40.0%
GAAAAAATCTCCCCTGTTCA+AGG + Chr7:88828534-88828553 MsG0780041253.01.T01:intron 40.0%
GCGATACAGTATTCAGTTCA+GGG - Chr7:88828714-88828733 None:intergenic 40.0%
GCTGATATCCAACAACAACT+TGG + Chr7:88829049-88829068 MsG0780041253.01.T01:CDS 40.0%
GTATATCAGCGGGCATAATT+TGG + Chr7:88829666-88829685 MsG0780041253.01.T01:CDS 40.0%
GTATCCAAGTTTGCGGTTAT+AGG + Chr7:88829409-88829428 MsG0780041253.01.T01:CDS 40.0%
GTTCACAATTAGCTCCTTTC+AGG - Chr7:88829597-88829616 None:intergenic 40.0%
TAGGCCAAAAGAAACCATAG+AGG + Chr7:88828829-88828848 MsG0780041253.01.T01:intron 40.0%
TCCATTTCGCTCATGACAAA+AGG - Chr7:88829514-88829533 None:intergenic 40.0%
TCCTTCAGTTTCTCTGTCTT+AGG + Chr7:88828621-88828640 MsG0780041253.01.T01:intron 40.0%
TGCAGCATTAGCGATATCAT+CGG - Chr7:88829620-88829639 None:intergenic 40.0%
TTAGATTAATCCAACGACCG+AGG + Chr7:88828805-88828824 MsG0780041253.01.T01:intron 40.0%
TTCGGCAAACTCTTGACTTT+GGG + Chr7:88828746-88828765 MsG0780041253.01.T01:intron 40.0%
TTGAAGCTCTCAGCAAACAA+AGG - Chr7:88828560-88828579 None:intergenic 40.0%
TTTCTTGGCCAAGTTGTTGT+TGG - Chr7:88829060-88829079 None:intergenic 40.0%
! TCCTTTTGTCATGAGCGAAA+TGG + Chr7:88829510-88829529 MsG0780041253.01.T01:CDS 40.0%
!! ACAAGGTAGCACTGATAACT+GGG + Chr7:88828960-88828979 MsG0780041253.01.T01:CDS 40.0%
!! TATCGCCTTTGAAACCTCTT+CGG + Chr7:88828728-88828747 MsG0780041253.01.T01:intron 40.0%
!!! GCATACCCCGTGATATTTTT+TGG - Chr7:88828101-88828120 None:intergenic 40.0%
AAGTCAAGAGTTTGCCGAAG+AGG - Chr7:88828745-88828764 None:intergenic 45.0%
AGATTGAGGTCAACAACGCT+TGG - Chr7:88829232-88829251 None:intergenic 45.0%
AGCAAACAAAGGCCTTGAAC+AGG - Chr7:88828549-88828568 None:intergenic 45.0%
CAAACAAAGGCCTTGAACAG+GGG - Chr7:88828547-88828566 None:intergenic 45.0%
CCACATCAGACGTGAATGAA+AGG - Chr7:88828921-88828940 None:intergenic 45.0%
CCTTTCATTCACGTCTGATG+TGG + Chr7:88828918-88828937 MsG0780041253.01.T01:intron 45.0%
CTTCGGCAAACTCTTGACTT+TGG + Chr7:88828745-88828764 MsG0780041253.01.T01:intron 45.0%
GCAAACAAAGGCCTTGAACA+GGG - Chr7:88828548-88828567 None:intergenic 45.0%
GCAGCATTAGCGATATCATC+GGG - Chr7:88829619-88829638 None:intergenic 45.0%
GCCTAAGACAGAGAAACTGA+AGG - Chr7:88828625-88828644 None:intergenic 45.0%
GGCGATACAGTATTCAGTTC+AGG - Chr7:88828715-88828734 None:intergenic 45.0%
GGGCATAATTTGGTTGTGGA+TGG + Chr7:88829676-88829695 MsG0780041253.01.T01:CDS 45.0%
GTTTGCCGAAGAGGTTTCAA+AGG - Chr7:88828736-88828755 None:intergenic 45.0%
TAATGCACTTGATCTGGTGC+AGG - Chr7:88829733-88829752 None:intergenic 45.0%
TACCTTGTCTTGTAGCTTCC+TGG - Chr7:88828948-88828967 None:intergenic 45.0%
TCGCTCATGACAAAAGGAGT+AGG - Chr7:88829508-88829527 None:intergenic 45.0%
TCTACTCAGACTGGAAGGTA+TGG + Chr7:88828590-88828609 MsG0780041253.01.T01:intron 45.0%
TGTGACGTCGCAAGTTATGA+AGG - Chr7:88829110-88829129 None:intergenic 45.0%
TTAATCCAACGACCGAGGTT+AGG + Chr7:88828810-88828829 MsG0780041253.01.T01:intron 45.0%
TTCATTCACGTCTGATGTGG+TGG + Chr7:88828921-88828940 MsG0780041253.01.T01:intron 45.0%
! CGTAATGTTGGTGTCCTGAA+AGG + Chr7:88829580-88829599 MsG0780041253.01.T01:CDS 45.0%
! GGTTTCTTTTGGCCTAACCT+CGG - Chr7:88828825-88828844 None:intergenic 45.0%
!! AAGGTAGCACTGATAACTGG+GGG + Chr7:88828962-88828981 MsG0780041253.01.T01:CDS 45.0%
!! CAAGGTAGCACTGATAACTG+GGG + Chr7:88828961-88828980 MsG0780041253.01.T01:CDS 45.0%
!! GACAAGGTAGCACTGATAAC+TGG + Chr7:88828959-88828978 MsG0780041253.01.T01:CDS 45.0%
AGAATGGATCCAGTTCCACG+CGG - Chr7:88829334-88829353 None:intergenic 50.0%
AGTCGCTGCGAATCAAGATG+TGG - Chr7:88829547-88829566 None:intergenic 50.0%
CAGCGGGCATAATTTGGTTG+TGG + Chr7:88829672-88829691 MsG0780041253.01.T01:CDS 50.0%
GTCCAGGAAGCTACAAGACA+AGG + Chr7:88828943-88828962 MsG0780041253.01.T01:intron 50.0%
TTGAGGTCAACAACGCTTGG+AGG - Chr7:88829229-88829248 None:intergenic 50.0%
! AACGCTTGGAGGAGTTTTGC+AGG - Chr7:88829218-88829237 None:intergenic 50.0%
! ACGCTTGGAGGAGTTTTGCA+GGG - Chr7:88829217-88829236 None:intergenic 50.0%
! ATGCATGGACTGTGGTGTGA+TGG - Chr7:88828072-88828091 None:intergenic 50.0%
! GGGATAAGATGCATGGACTG+TGG - Chr7:88828080-88828099 None:intergenic 50.0%
!! ATTGACTCGGATTCCATGCC+TGG - Chr7:88829464-88829483 None:intergenic 50.0%
!! GACTGTGGTGTGATGGACAT+AGG - Chr7:88828065-88828084 None:intergenic 50.0%
AATGTTCGCGGCGTTATGGC+TGG + Chr7:88829280-88829299 MsG0780041253.01.T01:CDS 55.0%
ACTGGGGGAGCTAGTGGAAT+AGG + Chr7:88828977-88828996 MsG0780041253.01.T01:CDS 55.0%
AGCGTCACTGGTGTGATTGG+AGG + Chr7:88829364-88829383 MsG0780041253.01.T01:CDS 55.0%
CACTGGTGTGATTGGAGGGA+TGG + Chr7:88829369-88829388 MsG0780041253.01.T01:CDS 55.0%
CCATGGCTTCAGAGCTATCC+AGG + Chr7:88829443-88829462 MsG0780041253.01.T01:CDS 55.0%
CTGATAACTGGGGGAGCTAG+TGG + Chr7:88828971-88828990 MsG0780041253.01.T01:CDS 55.0%
GAATGGATCCAGTTCCACGC+GGG - Chr7:88829333-88829352 None:intergenic 55.0%
GACGCTGGCAGTGCATAGAA+TGG - Chr7:88829350-88829369 None:intergenic 55.0%
GCGTCACTGGTGTGATTGGA+GGG + Chr7:88829365-88829384 MsG0780041253.01.T01:CDS 55.0%
GCTTCAGAGCTATCCAGGCA+TGG + Chr7:88829448-88829467 MsG0780041253.01.T01:CDS 55.0%
GTAATGATCCCGCGTGGAAC+TGG + Chr7:88829322-88829341 MsG0780041253.01.T01:CDS 55.0%
TCCAATCACACCAGTGACGC+TGG - Chr7:88829365-88829384 None:intergenic 55.0%
TTTGGCCTAACCTCGGTCGT+TGG - Chr7:88828818-88828837 None:intergenic 55.0%
!! CCTGGATAGCTCTGAAGCCA+TGG - Chr7:88829446-88829465 None:intergenic 55.0%
CACGTCTGATGTGGTGGTCC+AGG + Chr7:88828927-88828946 MsG0780041253.01.T01:intron 60.0%
CTATGCACTGCCAGCGTCAC+TGG + Chr7:88829352-88829371 MsG0780041253.01.T01:CDS 60.0%
GCCAGCGTCACTGGTGTGAT+TGG + Chr7:88829361-88829380 MsG0780041253.01.T01:CDS 60.0%
GCGCGTGTAATGATCCCGCG+TGG + Chr7:88829316-88829335 MsG0780041253.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 88828065 88829975 88828065 ID=MsG0780041253.01;Name=MsG0780041253.01
Chr7 mRNA 88828065 88829975 88828065 ID=MsG0780041253.01.T01;Parent=MsG0780041253.01;Name=MsG0780041253.01.T01;_AED=0.22;_eAED=0.25;_QI=0|0|0.33|1|0|0.66|3|222|310
Chr7 exon 88828065 88828114 88828065 ID=MsG0780041253.01.T01:exon:3401;Parent=MsG0780041253.01.T01
Chr7 exon 88828236 88828313 88828236 ID=MsG0780041253.01.T01:exon:3402;Parent=MsG0780041253.01.T01
Chr7 exon 88828949 88829975 88828949 ID=MsG0780041253.01.T01:exon:3403;Parent=MsG0780041253.01.T01
Chr7 CDS 88828065 88828114 88828065 ID=MsG0780041253.01.T01:cds;Parent=MsG0780041253.01.T01
Chr7 CDS 88828236 88828313 88828236 ID=MsG0780041253.01.T01:cds;Parent=MsG0780041253.01.T01
Chr7 CDS 88828949 88829753 88828949 ID=MsG0780041253.01.T01:cds;Parent=MsG0780041253.01.T01
Chr7 three_prime_UTR 88829754 88829975 88829754 ID=MsG0780041253.01.T01:three_prime_utr;Parent=MsG0780041253.01.T01
Gene Sequence

>MsG0780041253.01.T01

ATGTCCATCACACCACAGTCCATGCATCTTATCCCAAAAAATATCACGGGGGACTCAAACTACATTACACTCCAGTGTGTGAGATCTAATTCATTTTGGCAGAAAGTCATGTTAAGAATTTGTTTGAGGAAGCTACAAGACAAGGTAGCACTGATAACTGGGGGAGCTAGTGGAATAGGAAAAGCTGCAGCAACAAAATTCATCAATAATGGAGCAAAAGTAATTATTGCTGATATCCAACAACAACTTGGCCAAGAAACTGCAAAAGAACTAGGACCTAATGCTACCTTCATAACTTGCGACGTCACAAAAGAATCAGACATATCTGACGCAGTTGATTTCACCGTTTCTGAATACAAACAACTTGATATCATGTATAACAATGCAGGGATACCCTGCAAAACTCCTCCAAGCGTTGTTGACCTCAATCTAGAATCGTTTGACAAAGTCATGGAAATAAATGTTCGCGGCGTTATGGCTGGCATCAAGCATGCAGCGCGTGTAATGATCCCGCGTGGAACTGGATCCATTCTATGCACTGCCAGCGTCACTGGTGTGATTGGAGGGATGGCGCAGCATACATACTCTGTATCCAAGTTTGCGGTTATAGGCATTGTTAAATCCATGGCTTCAGAGCTATCCAGGCATGGAATCCGAGTCAATTGTATATCGCCTTTTGCTATTCCTACTCCTTTTGTCATGAGCGAAATGGATCAGATATATCCACATCTTGATTCGCAGCGACTTGTAGAAATTGTTCGTAATGTTGGTGTCCTGAAAGGAGCTAATTGTGAACCCGATGATATCGCTAATGCTGCACTTTACCTTGCATCTAATGATGCTAGGTATATCAGCGGGCATAATTTGGTTGTGGATGGAGGTTTCACATCTTTTAAGAATTTGGAGTTTCCTGCACCAGATCAAGTGCATTAG
Protein sequence

>MsG0780041253.01.T01

MSITPQSMHLIPKNITGDSNYITLQCVRSNSFWQKVMLRICLRKLQDKVALITGGASGIGKAAATKFINNGAKVIIADIQQQLGQETAKELGPNATFITCDVTKESDISDAVDFTVSEYKQLDIMYNNAGIPCKTPPSVVDLNLESFDKVMEINVRGVMAGIKHAARVMIPRGTGSILCTASVTGVIGGMAQHTYSVSKFAVIGIVKSMASELSRHGIRVNCISPFAIPTPFVMSEMDQIYPHLDSQRLVEIVRNVGVLKGANCEPDDIANAALYLASNDARYISGHNLVVDGGFTSFKNLEFPAPDQVH*