AlfalfaGEDB | MsG0580024778.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024778.01

KEGG: K14487
GO: -
PFAM: PF03321

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024778.01.T01	XP_003611653.1	96.667	600	20	0	1	600	1	600	0	1201

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024778.01.T01	O82333	74.959	603	134	4	1	600	1	589	0	942

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380015009.01	MsG0580024778.01	0.816449	5.500020e-52	3.898071e-49
MsG0480019217.01	MsG0580024778.01	0.802216	6.232879e-49	3.035896e-46
MsG0580024778.01	MsG0680034317.01	0.803940	2.742415e-49	1.396121e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024778.01.T01	MTR_5g016320	96.667	600	20	0	1	600	1	600	0.0	1201
MsG0580024778.01.T01	MTR_0035s0150	77.741	602	118	4	1	600	1	588	0.0	959
MsG0580024778.01.T01	MTR_8g467000	63.481	586	203	3	24	600	18	601	0.0	788
MsG0580024778.01.T01	MTR_2g081860	60.199	603	225	4	12	600	2	603	0.0	760
MsG0580024778.01.T01	MTR_4g045903	60.757	581	221	4	23	600	32	608	0.0	752
MsG0580024778.01.T01	MTR_1g088765	53.425	584	262	5	23	596	13	596	0.0	635
MsG0580024778.01.T01	MTR_1g088765	53.319	452	203	4	153	596	1	452	4.54e-171	494
MsG0580024778.01.T01	MTR_7g117110	39.587	581	340	6	15	591	5	578	8.80e-146	434
MsG0580024778.01.T01	MTR_8g027970	38.554	581	340	6	15	590	5	573	1.06e-142	426
MsG0580024778.01.T01	MTR_8g027920	37.629	582	344	8	15	590	5	573	1.05e-141	423

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0580024778.01.T01	AT2G14960	74.959	603	134	4	1	600	1	589	942
MsG0580024778.01.T01	AT2G23170	75.372	605	134	6	1	600	1	595	931
MsG0580024778.01.T01	AT4G37390	74.257	606	147	4	1	600	1	603	926
MsG0580024778.01.T01	AT1G59500	69.934	602	174	4	1	600	1	597	853
MsG0580024778.01.T01	AT5G54510	64.094	596	202	5	14	600	16	608	801
MsG0580024778.01.T01	AT4G27260	64.034	595	204	4	14	600	16	608	801
MsG0580024778.01.T01	AT1G28130	53.488	602	260	7	14	596	4	604	662
MsG0580024778.01.T01	AT2G47750	50.000	584	277	8	21	596	9	585	590
MsG0580024778.01.T01	AT1G23160	47.945	584	285	6	13	588	5	577	561
MsG0580024778.01.T01	AT5G13320	46.573	569	288	4	27	588	30	589	546

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Find 126 sgRNAs with CRISPR-Local

Find 144 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCTGAGATTGGATGAG+AGG	0.584597	5:+9579510	None:intergenic
AAATGAAGCTATCCTTTGTC+TGG	0.396144	5:-9578955	MsG0580024778.01.T01:CDS
AAATGCTTCTGAATTGTTAA+AGG	0.206551	5:-9578169	MsG0580024778.01.T01:CDS
AAATTAGCGAGTTCGGGATC+CGG	0.454702	5:+9578717	None:intergenic
AACACATTGTAAGGGTCAAA+TGG	0.480939	5:+9578987	None:intergenic
AACGTATAATCCCAAGAATC+TGG	0.429037	5:-9578662	MsG0580024778.01.T01:CDS
AAGAATGTTCAGGAGACAAT+TGG	0.367565	5:-9578686	MsG0580024778.01.T01:CDS
AAGATCTTCATAGGTGATAA+CGG	0.566927	5:+9579582	None:intergenic
AATGGAACTTCTTGACTCTA+GGG	0.522711	5:-9577824	MsG0580024778.01.T01:CDS
AATTCAAAGTAACCCATGTT+TGG	0.385809	5:+9578459	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATCTTCATAGGTGATAA+CGG	+	Chr5:9577988-9578007	None:intergenic	30.0%
AATTCAAAGTAACCCATGTT+TGG	+	Chr5:9579111-9579130	None:intergenic	30.0%
AGAGTAGCTGATAATTCAAT+TGG	-	Chr5:9579600-9579619	MsG0580024778.01.T01:CDS	30.0%
ATCCTCAATTCAAATTTGTG+AGG	-	Chr5:9579307-9579326	MsG0580024778.01.T01:CDS	30.0%
CAATAGATAACATAATGTCC+AGG	+	Chr5:9579477-9579496	None:intergenic	30.0%
CACAAATTTGAATTGAGGAT+CGG	+	Chr5:9579307-9579326	None:intergenic	30.0%
CATGTCCAAAATACTTAAAC+CGG	-	Chr5:9578831-9578850	MsG0580024778.01.T01:CDS	30.0%
CTGGACATTATGTTATCTAT+TGG	-	Chr5:9579475-9579494	MsG0580024778.01.T01:intron	30.0%
CTTTGGATTTGAAAGTTTCA+CGG	+	Chr5:9577962-9577981	None:intergenic	30.0%
GTTACCACTGTAATAATCAA+GGG	+	Chr5:9578989-9579008	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	9577765	9579824	9577765	ID=MsG0580024778.01;Name=MsG0580024778.01
Chr5	mRNA	9577765	9579824	9577765	ID=MsG0580024778.01.T01;Parent=MsG0580024778.01;Name=MsG0580024778.01.T01;_AED=0.14;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|3\|0\|600
Chr5	exon	9579502	9579824	9579502	ID=MsG0580024778.01.T01:exon:26509;Parent=MsG0580024778.01.T01
Chr5	exon	9579274	9579375	9579274	ID=MsG0580024778.01.T01:exon:26508;Parent=MsG0580024778.01.T01
Chr5	exon	9577765	9579142	9577765	ID=MsG0580024778.01.T01:exon:26507;Parent=MsG0580024778.01.T01
Chr5	CDS	9579502	9579824	9579502	ID=MsG0580024778.01.T01:cds;Parent=MsG0580024778.01.T01
Chr5	CDS	9579274	9579375	9579274	ID=MsG0580024778.01.T01:cds;Parent=MsG0580024778.01.T01
Chr5	CDS	9577765	9579142	9577765	ID=MsG0580024778.01.T01:cds;Parent=MsG0580024778.01.T01

Gene Sequence

>MsG0580024778.01.T01

ATGGCTGTTGAATCTGAAATCCTTTTGGAACCACCTTCTTCTGTTGATGAAAAGAGTCTAAACAATGCTCTTGAATTCATTGAAGAAATGACCATAAACACTGACTCAGTTCAAGAGAGAGTTCTCTCTGAGATTCTTGCACAAAACGCAGAAACTGAGTACTTGAAACGGTTTGGACTTAACGGTGCCACTGACCGTGAAACTTTCAAATCCAAAGTCGCCGTTATCACCTATGAAGATCTTTCACCTGACATTCAACGCATTGCTAACGGTGACACTTCTCCGATCTTGTCCTCTCATCCAATCTCAGAGTTTCTGACCAGTTCAGGAACATCAGCTGGTGAAAGAAAATTGATGCCTACTATTCATCAAGAAATGGACCGTCGTCAGTTATTGTACAGCCTACTCATGCCAGTGATGAACCAATATGTTCCTGATTTGGACAAAGGAAAAGCACTTCACTTTCTTTTCATCAAAGCAGAAACAAAAACTCCAAGTGGGTTAGTGGCACGTCCAGTTCTAACAGCTTACTACAAAAGTGAACAGTTCAAGAAAAGACCATTTGACCCTTACAATGTGTTAACAAGTCCAAATGAAGCTATCCTTTGTCTGGATTCATTTCAAAGCATGTATACTCAGATGCTTTGTGGCCTAATCATGCGCCATGAAGTTCTTCGAGTCGGAGCGGTTTTCGCTTCCGGGCTTCTCCGAGCCATTAGGTTCCTTCAGCTCAACTGGGCTGAATTAGTCCATGACATTGAAACAGGAACCCTAAACCCAAAAATCACTGATCCTTCTATCAAACAGTGCATGTCCAAAATACTTAAACCGGATCCCGAACTCGCTAATTTTGTTACAAAAGAATGTTCAGGAGACAATTGGGAACGTATAATCCCAAGAATCTGGCCTAACACAAAGTATCTTGAGGTTATTGTCACTGGTGCTATGGCTCAGTACATTCCTACCCTTGATTATTACAGTGGTAACCTACCAAAGCCATGTACAATGTATGCTTCATCTGAATGTTATTTTGGTCTTAACCTTAAGCCAATGACAGAACCTAATGAAGTGTCTTACACCATTATGCCAAACATGGGTTACTTTGAATTTTTACCACATGATGATTCATCACCTATAACCCTCTCACGTGACTCACCTCCAAAGCTAGTAGACCTTGCTGATGTTGAAATCGGTAAATCTTATGAGCTTATAATCACAACTTATTCAGGTCTTTGCCGTTACAGAGTTGGTGATATTCTTCAAGTCACAGGATTCCATAACTCCGATCCTCAATTCAAATTTGTGAGGCGAAAGAACGTGTTGTTGAGTATCGATTCGGATAAAACAGACGAATCGGAGCTTCAAAAAGCTATTGAAAATGCTTCTGAATTGTTAAAGGAGTTCAAAACAAGTGTTGTTGAATACACTAGTTTTGCTGATACAAAATCCATTCCTGGACATTATGTTATCTATTGGGAGCTTTTGATGAAAGATTCCTCTGATCCTCCAACAGAAGAAGTGTTGAATCAATGTTGTTTGGTTATGGAAGAGTCTTTGAATTCTGTTTATAGACAAGGAAGAGTAGCTGATAATTCAATTGGACCATTAGAGATACGTGTGGTGAAAAATGGCACGTTTGAGGAACTAATGGATTATGCAATATCGCGTGGTGCAAGCATTAATCAGTATAAAGTTCCTAGGTGCGTGAGTTTCACTCCTATAATGGAACTTCTTGACTCTAGGGTGGTTTCAGTTCATTTTAGTCCTTCTCTACCTCATTGGACTCCAGAACGACGTCGTTGA

Protein sequence

>MsG0580024778.01.T01

MAVESEILLEPPSSVDEKSLNNALEFIEEMTINTDSVQERVLSEILAQNAETEYLKRFGLNGATDRETFKSKVAVITYEDLSPDIQRIANGDTSPILSSHPISEFLTSSGTSAGERKLMPTIHQEMDRRQLLYSLLMPVMNQYVPDLDKGKALHFLFIKAETKTPSGLVARPVLTAYYKSEQFKKRPFDPYNVLTSPNEAILCLDSFQSMYTQMLCGLIMRHEVLRVGAVFASGLLRAIRFLQLNWAELVHDIETGTLNPKITDPSIKQCMSKILKPDPELANFVTKECSGDNWERIIPRIWPNTKYLEVIVTGAMAQYIPTLDYYSGNLPKPCTMYASSECYFGLNLKPMTEPNEVSYTIMPNMGYFEFLPHDDSSPITLSRDSPPKLVDLADVEIGKSYELIITTYSGLCRYRVGDILQVTGFHNSDPQFKFVRRKNVLLSIDSDKTDESELQKAIENASELLKEFKTSVVEYTSFADTKSIPGHYVIYWELLMKDSSDPPTEEVLNQCCLVMEESLNSVYRQGRVADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSVHFSPSLPHWTPERRR*