AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034317.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034317.01.T01 MTR_6g059530 93.596 203 11 1 1 201 1 203 4.15e-126 354
MsG0680034317.01.T01 MTR_6g059410 92.611 203 12 2 1 201 1 202 6.86e-124 348
MsG0680034317.01.T01 MTR_6g059650 56.186 194 75 5 17 201 19 211 7.25e-66 202
MsG0680034317.01.T01 MTR_6g065460 54.854 206 78 7 1 193 1 204 1.43e-57 180
MsG0680034317.01.T01 MTR_6g059760 49.282 209 89 11 1 196 1 205 2.00e-49 160
MsG0680034317.01.T01 MTR_6g065460 49.754 203 69 6 1 193 1 180 1.67e-47 154
MsG0680034317.01.T01 MTR_6g044780 43.256 215 103 10 1 199 1 212 2.91e-42 142
MsG0680034317.01.T01 MTR_6g059680 48.020 202 86 8 16 201 18 216 4.83e-42 141
MsG0680034317.01.T01 MTR_3g463590 43.750 192 95 7 20 201 23 211 2.20e-40 137
MsG0680034317.01.T01 MTR_6g045097 42.326 215 105 10 1 199 1 212 2.92e-40 137
MsG0680034317.01.T01 MTR_6g044810 42.326 215 105 10 1 199 1 212 2.45e-39 134
MsG0680034317.01.T01 MTR_6g059730 45.116 215 101 9 1 201 1 212 2.31e-38 132
MsG0680034317.01.T01 MTR_6g009650 43.750 208 98 9 1 194 1 203 1.04e-37 130
MsG0680034317.01.T01 MTR_6g045403 41.121 214 107 9 1 199 1 210 3.61e-37 129
MsG0680034317.01.T01 MTR_6g066190 41.148 209 104 9 1 195 1 204 1.59e-35 124
MsG0680034317.01.T01 MTR_6g017225 41.364 220 106 12 1 201 1 216 2.27e-35 128
MsG0680034317.01.T01 MTR_6g009563 41.627 209 102 9 1 194 1 204 9.62e-34 119
MsG0680034317.01.T01 MTR_6g066210 40.566 212 111 8 1 199 1 210 1.39e-32 117
MsG0680034317.01.T01 MTR_6g045433 36.797 231 104 11 1 201 1 219 1.09e-31 115
MsG0680034317.01.T01 MTR_6g059810 40.097 207 98 10 4 190 8 208 5.43e-31 112
MsG0680034317.01.T01 MTR_1g035770 40.291 206 101 10 6 192 7 209 4.66e-27 102
MsG0680034317.01.T01 MTR_6g059755 50.000 108 48 4 98 199 18 125 3.89e-26 97.8
MsG0680034317.01.T01 MTR_6g078070 32.683 205 124 8 1 193 1 203 7.59e-21 86.3
MsG0680034317.01.T01 MTR_6g078100 33.173 208 121 9 1 193 1 205 4.10e-19 81.6
MsG0680034317.01.T01 MTR_1g057110 37.500 208 95 13 1 194 1 187 4.54e-19 81.3
MsG0680034317.01.T01 MTR_6g065570 53.333 60 25 1 16 72 14 73 1.72e-15 69.3
MsG0680034317.01.T01 MTR_0100s0150 26.471 204 120 8 7 190 5 198 3.84e-14 68.2
MsG0680034317.01.T01 MTR_3g106585 32.000 200 119 9 5 194 6 198 4.38e-14 68.6
MsG0680034317.01.T01 MTR_0084s0030 29.703 202 123 8 5 190 1 199 3.83e-13 65.5
MsG0680034317.01.T01 MTR_3g106620 31.492 181 107 9 24 194 27 200 6.19e-13 65.5
MsG0680034317.01.T01 MTR_0302s0030 27.273 209 119 8 5 190 1 199 1.17e-12 64.3
MsG0680034317.01.T01 MTR_6g078290 30.732 205 112 10 11 190 10 209 3.76e-12 63.2
MsG0680034317.01.T01 MTR_0211s0080 27.778 198 128 7 5 190 1 195 1.02e-11 61.6
MsG0680034317.01.T01 MTR_2g073010 28.218 202 127 7 1 193 6 198 4.40e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034317.01.T01 AT1G73260 35.326 184 105 7 25 197 29 209 2.38e-22 90.5
MsG0680034317.01.T01 AT1G17860 31.492 181 107 7 21 193 24 195 4.34e-16 73.6

Find 52 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTGAAAATGATCCATTT+AGG 0.174286 6:+85585899 MsG0680034317.01.T01:CDS
TCAACAAATACAACCCTAAA+TGG 0.255135 6:-85585913 None:intergenic
GAAACGGTTGAAGACATAAA+TGG 0.296997 6:+85585430 MsG0680034317.01.T01:CDS
AACTGAAAATGATCCATTTA+GGG 0.299087 6:+85585900 MsG0680034317.01.T01:CDS
CCTACATCTTGGGTTGGTAT+TGG 0.301766 6:+85585724 MsG0680034317.01.T01:CDS
GGTAAGCAATGTTGGCTTCA+TGG 0.309270 6:-85585359 None:intergenic
AAATCTTGTAGAACAGTAAC+TGG 0.355500 6:-85585553 None:intergenic
TGATGATTTCCCTACATCTT+GGG 0.363632 6:+85585714 MsG0680034317.01.T01:CDS
CCTGGTGCTTGTAGTGATAT+TGG 0.364762 6:+85585841 MsG0680034317.01.T01:CDS
ACCGTTTCAGTAAAAGCTAA+TGG 0.365801 6:-85585415 None:intergenic
AATGGAAACCCCGTATTTCC+TGG 0.375075 6:+85585448 MsG0680034317.01.T01:CDS
ACTCGTATGATAAATCTAAC+TGG 0.385923 6:-85585598 None:intergenic
AAAATTGAGACACTTGGTTC+AGG 0.392743 6:+85585778 MsG0680034317.01.T01:CDS
ATGATGATTTCCCTACATCT+TGG 0.402726 6:+85585713 MsG0680034317.01.T01:CDS
GATAATATTGATGCATTTCA+AGG 0.419889 6:+85585748 MsG0680034317.01.T01:CDS
CTGGTGCTTGTAGTGATATT+GGG 0.426790 6:+85585842 MsG0680034317.01.T01:CDS
ATCATGAGCTAGCACCCATT+TGG 0.447134 6:-85585695 None:intergenic
GTATTTGCTACCAGGAAATA+CGG 0.450228 6:-85585458 None:intergenic
TATTTGCTACCAGGAAATAC+GGG 0.456020 6:-85585457 None:intergenic
GAAAGTAGGTAAAGGAAAAG+AGG 0.466771 6:-85585393 None:intergenic
AGATGAGAATGGATGGCGTT+TGG 0.471954 6:+85585873 MsG0680034317.01.T01:CDS
GATTTCCCTACATCTTGGGT+TGG 0.486617 6:+85585718 MsG0680034317.01.T01:CDS
CCAATACCAACCCAAGATGT+AGG 0.489601 6:-85585724 None:intergenic
AGTAAAAGCTAATGGAAAGT+AGG 0.489860 6:-85585407 None:intergenic
GGATTGAAGCTTGGTAAGAC+AGG 0.499062 6:+85585517 MsG0680034317.01.T01:CDS
GGAAAAGAGGAGGAGAGAAA+AGG 0.510811 6:-85585380 None:intergenic
ATGTCTAAAGGAGGAGGTGG+TGG 0.513667 6:+85585496 MsG0680034317.01.T01:CDS
GGAGGTGGTGGATTGAAGCT+TGG 0.515566 6:+85585508 MsG0680034317.01.T01:CDS
GGGAGGTATAGAGATGAGAA+TGG 0.518670 6:+85585862 MsG0680034317.01.T01:CDS
TTGGAAGATTCAGCACACTT+TGG 0.519328 6:-85585676 None:intergenic
CCTCCTCCTTTAGACATAAG+TGG 0.521949 6:-85585490 None:intergenic
CCAAGGTTCAGTGCTCCACC+TGG 0.525036 6:+85585823 MsG0680034317.01.T01:CDS
AGAGAAAAGGTAAGCAATGT+TGG 0.528481 6:-85585367 None:intergenic
ATTGCACCACTTATGTCTAA+AGG 0.535923 6:+85585484 MsG0680034317.01.T01:CDS
AGTGTGCTGAATCTTCCAAA+TGG 0.536599 6:+85585680 MsG0680034317.01.T01:CDS
AGCTAATGGAAAGTAGGTAA+AGG 0.537202 6:-85585401 None:intergenic
CAATACCAACCCAAGATGTA+GGG 0.544334 6:-85585723 None:intergenic
CCAGGTGGAGCACTGAACCT+TGG 0.547779 6:-85585823 None:intergenic
AAATTTAAAATTGAGACACT+TGG 0.558333 6:+85585772 MsG0680034317.01.T01:CDS
ACAAGCTTGTGTATTGTCCA+AGG 0.573799 6:+85585806 MsG0680034317.01.T01:CDS
GTGTGCTGAATCTTCCAAAT+GGG 0.579792 6:+85585681 MsG0680034317.01.T01:CDS
CTTATGTCTAAAGGAGGAGG+TGG 0.581977 6:+85585493 MsG0680034317.01.T01:CDS
AGTAGGTAAAGGAAAAGAGG+AGG 0.584445 6:-85585390 None:intergenic
CCACTTATGTCTAAAGGAGG+AGG 0.587624 6:+85585490 MsG0680034317.01.T01:CDS
GGTATAGAGATGAGAATGGA+TGG 0.592483 6:+85585866 MsG0680034317.01.T01:CDS
GCAATATAGTATTTGCTACC+AGG 0.595672 6:-85585466 None:intergenic
CCAATATCACTACAAGCACC+AGG 0.612889 6:-85585841 None:intergenic
GCACCACTTATGTCTAAAGG+AGG 0.661209 6:+85585487 MsG0680034317.01.T01:CDS
ATTTGCTACCAGGAAATACG+GGG 0.675453 6:-85585456 None:intergenic
GTGCTTGTAGTGATATTGGG+AGG 0.713327 6:+85585845 MsG0680034317.01.T01:CDS
ATATCACTACAAGCACCAGG+TGG 0.748505 6:-85585838 None:intergenic
TTTATCATACGAGTAAAACG+TGG 0.798123 6:+85585607 MsG0680034317.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTAAAATTGAGACACT+TGG + Chr6:85585772-85585791 MsG0680034317.01.T01:CDS 20.0%
! AACTGAAAATGATCCATTTA+GGG + Chr6:85585900-85585919 MsG0680034317.01.T01:CDS 25.0%
! GATAATATTGATGCATTTCA+AGG + Chr6:85585748-85585767 MsG0680034317.01.T01:CDS 25.0%
!! TAAATGGATCATTTTCAGTT+GGG - Chr6:85585900-85585919 None:intergenic 25.0%
!!! TGTTCTACAAGATTTTAATG+AGG + Chr6:85585561-85585580 MsG0680034317.01.T01:CDS 25.0%
AAATCTTGTAGAACAGTAAC+TGG - Chr6:85585556-85585575 None:intergenic 30.0%
ACTCGTATGATAAATCTAAC+TGG - Chr6:85585601-85585620 None:intergenic 30.0%
AGTAAAAGCTAATGGAAAGT+AGG - Chr6:85585410-85585429 None:intergenic 30.0%
CAACTGAAAATGATCCATTT+AGG + Chr6:85585899-85585918 MsG0680034317.01.T01:CDS 30.0%
TCAACAAATACAACCCTAAA+TGG - Chr6:85585916-85585935 None:intergenic 30.0%
TTTATCATACGAGTAAAACG+TGG + Chr6:85585607-85585626 MsG0680034317.01.T01:CDS 30.0%
! CTAAATGGATCATTTTCAGT+TGG - Chr6:85585901-85585920 None:intergenic 30.0%
!! GATTTTAATGAGGTTGTTAG+AGG + Chr6:85585571-85585590 MsG0680034317.01.T01:CDS 30.0%
!!! TCCATTAGCTTTTACTGAAA+CGG + Chr6:85585414-85585433 MsG0680034317.01.T01:CDS 30.0%
AAAATTGAGACACTTGGTTC+AGG + Chr6:85585778-85585797 MsG0680034317.01.T01:CDS 35.0%
ACCGTTTCAGTAAAAGCTAA+TGG - Chr6:85585418-85585437 None:intergenic 35.0%
AGAGAAAAGGTAAGCAATGT+TGG - Chr6:85585370-85585389 None:intergenic 35.0%
AGCTAATGGAAAGTAGGTAA+AGG - Chr6:85585404-85585423 None:intergenic 35.0%
ATGATGATTTCCCTACATCT+TGG + Chr6:85585713-85585732 MsG0680034317.01.T01:CDS 35.0%
ATTGCACCACTTATGTCTAA+AGG + Chr6:85585484-85585503 MsG0680034317.01.T01:CDS 35.0%
GAAACGGTTGAAGACATAAA+TGG + Chr6:85585430-85585449 MsG0680034317.01.T01:CDS 35.0%
GAAAGTAGGTAAAGGAAAAG+AGG - Chr6:85585396-85585415 None:intergenic 35.0%
TGATGATTTCCCTACATCTT+GGG + Chr6:85585714-85585733 MsG0680034317.01.T01:CDS 35.0%
! GCAATATAGTATTTGCTACC+AGG - Chr6:85585469-85585488 None:intergenic 35.0%
! GTATTTGCTACCAGGAAATA+CGG - Chr6:85585461-85585480 None:intergenic 35.0%
! TATTTGCTACCAGGAAATAC+GGG - Chr6:85585460-85585479 None:intergenic 35.0%
ACAAGCTTGTGTATTGTCCA+AGG + Chr6:85585806-85585825 MsG0680034317.01.T01:CDS 40.0%
AGTAGGTAAAGGAAAAGAGG+AGG - Chr6:85585393-85585412 None:intergenic 40.0%
AGTGTGCTGAATCTTCCAAA+TGG + Chr6:85585680-85585699 MsG0680034317.01.T01:CDS 40.0%
CAATACCAACCCAAGATGTA+GGG - Chr6:85585726-85585745 None:intergenic 40.0%
CTGGTGCTTGTAGTGATATT+GGG + Chr6:85585842-85585861 MsG0680034317.01.T01:CDS 40.0%
GGTATAGAGATGAGAATGGA+TGG + Chr6:85585866-85585885 MsG0680034317.01.T01:CDS 40.0%
GTGTGCTGAATCTTCCAAAT+GGG + Chr6:85585681-85585700 MsG0680034317.01.T01:CDS 40.0%
TTGGAAGATTCAGCACACTT+TGG - Chr6:85585679-85585698 None:intergenic 40.0%
! ATTTGCTACCAGGAAATACG+GGG - Chr6:85585459-85585478 None:intergenic 40.0%
AATGGAAACCCCGTATTTCC+TGG + Chr6:85585448-85585467 MsG0680034317.01.T01:CDS 45.0%
AGATGAGAATGGATGGCGTT+TGG + Chr6:85585873-85585892 MsG0680034317.01.T01:CDS 45.0%
ATATCACTACAAGCACCAGG+TGG - Chr6:85585841-85585860 None:intergenic 45.0%
ATCATGAGCTAGCACCCATT+TGG - Chr6:85585698-85585717 None:intergenic 45.0%
CCAATACCAACCCAAGATGT+AGG - Chr6:85585727-85585746 None:intergenic 45.0%
CCAATATCACTACAAGCACC+AGG - Chr6:85585844-85585863 None:intergenic 45.0%
CCACTTATGTCTAAAGGAGG+AGG + Chr6:85585490-85585509 MsG0680034317.01.T01:CDS 45.0%
CCTCCTCCTTTAGACATAAG+TGG - Chr6:85585493-85585512 None:intergenic 45.0%
CCTGGTGCTTGTAGTGATAT+TGG + Chr6:85585841-85585860 MsG0680034317.01.T01:CDS 45.0%
CTTATGTCTAAAGGAGGAGG+TGG + Chr6:85585493-85585512 MsG0680034317.01.T01:CDS 45.0%
GATTTCCCTACATCTTGGGT+TGG + Chr6:85585718-85585737 MsG0680034317.01.T01:CDS 45.0%
GCACCACTTATGTCTAAAGG+AGG + Chr6:85585487-85585506 MsG0680034317.01.T01:CDS 45.0%
GGAAAAGAGGAGGAGAGAAA+AGG - Chr6:85585383-85585402 None:intergenic 45.0%
GGATTGAAGCTTGGTAAGAC+AGG + Chr6:85585517-85585536 MsG0680034317.01.T01:CDS 45.0%
GTGCTTGTAGTGATATTGGG+AGG + Chr6:85585845-85585864 MsG0680034317.01.T01:CDS 45.0%
! GGGAGGTATAGAGATGAGAA+TGG + Chr6:85585862-85585881 MsG0680034317.01.T01:CDS 45.0%
!! CCTACATCTTGGGTTGGTAT+TGG + Chr6:85585724-85585743 MsG0680034317.01.T01:CDS 45.0%
ATGTCTAAAGGAGGAGGTGG+TGG + Chr6:85585496-85585515 MsG0680034317.01.T01:CDS 50.0%
!! GGAGGTGGTGGATTGAAGCT+TGG + Chr6:85585508-85585527 MsG0680034317.01.T01:CDS 55.0%
CCAAGGTTCAGTGCTCCACC+TGG + Chr6:85585823-85585842 MsG0680034317.01.T01:CDS 60.0%
!! CCAGGTGGAGCACTGAACCT+TGG - Chr6:85585826-85585845 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 85585361 85585966 85585361 ID=MsG0680034317.01;Name=MsG0680034317.01
Chr6 mRNA 85585361 85585966 85585361 ID=MsG0680034317.01.T01;Parent=MsG0680034317.01;Name=MsG0680034317.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|201
Chr6 exon 85585361 85585966 85585361 ID=MsG0680034317.01.T01:exon:18050;Parent=MsG0680034317.01.T01
Chr6 CDS 85585361 85585966 85585361 ID=MsG0680034317.01.T01:cds;Parent=MsG0680034317.01.T01
Gene Sequence

>MsG0680034317.01.T01

ATGAAGCCAACATTGCTTACCTTTTCTCTCCTCCTCTTTTCCTTTACCTACTTTCCATTAGCTTTTACTGAAACGGTTGAAGACATAAATGGAAACCCCGTATTTCCTGGTAGCAAATACTATATTGCACCACTTATGTCTAAAGGAGGAGGTGGTGGATTGAAGCTTGGTAAGACAGGAAACTCAGAATGTCCAGTTACTGTTCTACAAGATTTTAATGAGGTTGTTAGAGGCTTGCCAGTTAGATTTATCATACGAGTAAAACGTGGTGTTATCTTTACAACTGACGAAGTAGATATTGAGTTTGTTAAGAAACCAAAGTGTGCTGAATCTTCCAAATGGGTGCTAGCTCATGATGATTTCCCTACATCTTGGGTTGGTATTGGTGATAATATTGATGCATTTCAAGGTAAATTTAAAATTGAGACACTTGGTTCAGGAGCATACAAGCTTGTGTATTGTCCAAGGTTCAGTGCTCCACCTGGTGCTTGTAGTGATATTGGGAGGTATAGAGATGAGAATGGATGGCGTTTGGTCCCAACTGAAAATGATCCATTTAGGGTTGTATTTGTTGATGCTACTGAATCTGAAAAAGCTGTAGTTTGA

Protein sequence

>MsG0680034317.01.T01

MKPTLLTFSLLLFSFTYFPLAFTETVEDINGNPVFPGSKYYIAPLMSKGGGGGLKLGKTGNSECPVTVLQDFNEVVRGLPVRFIIRVKRGVIFTTDEVDIEFVKKPKCAESSKWVLAHDDFPTSWVGIGDNIDAFQGKFKIETLGSGAYKLVYCPRFSAPPGACSDIGRYRDENGWRLVPTENDPFRVVFVDATESEKAVV*