AlfalfaGEDB | MsG0580025023.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025023.01

KEGG: K00430
GO: GO:0004601 GO:0006979 GO:0020037 GO:0042744 GO:0055114
PFAM: PF00141 PF00650

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025023.01.T01	XP_003612077.1	64.182	483	56	11	1	377	1	472	0	543

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025023.01.T01	Q9FJZ9	64.262	305	57	2	381	634	31	334	6.26E-133	396

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025023.01.T01	M4D5C6	46.738	751	241	17	10	634	6	723	0.0	573

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047831.01	MsG0580025023.01	0.815956	7.086948e-52	4.955879e-49
MsG0080047967.01	MsG0580025023.01	-0.832479	9.233320e-56	1.033299e-52
MsG0080048036.01	MsG0580025023.01	0.818876	1.559247e-52	1.181388e-49
MsG0080048134.01	MsG0580025023.01	0.833398	5.456219e-56	6.276131e-53
MsG0080048347.01	MsG0580025023.01	0.813815	2.114057e-51	1.394783e-48
MsG0080048352.01	MsG0580025023.01	0.803213	3.881746e-49	1.939382e-46
MsG0080048486.01	MsG0580025023.01	0.822175	2.726909e-53	2.265194e-50
MsG0080048487.01	MsG0580025023.01	0.824715	6.945179e-54	6.201972e-51
MsG0080048488.01	MsG0580025023.01	0.823846	1.111641e-53	9.683093e-51
MsG0080048489.01	MsG0580025023.01	0.825053	5.779565e-54	5.210845e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025023.01.T01	MTR_5g021050	64.182	483	56	11	1	377	1	472	0.0	543
MsG0580025023.01.T01	MTR_5g021050	64.182	483	56	11	1	377	1	472	0.0	542
MsG0580025023.01.T01	MTR_5g021060	79.935	309	12	2	376	634	23	331	3.98e-169	485
MsG0580025023.01.T01	MTR_8g076820	68.301	306	47	2	378	633	25	330	1.92e-142	417
MsG0580025023.01.T01	MTR_1g022970	50.993	302	97	3	383	633	46	347	3.47e-90	283
MsG0580025023.01.T01	MTR_4g029170	47.039	304	99	5	383	633	27	321	3.23e-80	257
MsG0580025023.01.T01	MTR_7g093370	43.894	303	118	5	383	633	69	371	3.20e-79	256
MsG0580025023.01.T01	MTR_2g084000	42.951	305	117	2	379	633	22	319	3.16e-78	251
MsG0580025023.01.T01	MTR_2g084010	42.020	307	121	2	377	633	19	318	1.82e-77	249
MsG0580025023.01.T01	MTR_2g084080	42.020	307	120	3	377	633	19	317	4.07e-77	248

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025023.01.T01	AT5G66390	64.262	305	57	2	381	634	31	334	6.39e-134	396
MsG0580025023.01.T01	AT2G18150	59.868	304	72	2	381	634	34	337	6.39e-122	365
MsG0580025023.01.T01	AT4G36430	57.566	304	79	2	381	634	28	331	2.80e-117	353
MsG0580025023.01.T01	AT2G18140	57.616	302	78	2	383	634	35	336	5.94e-117	352
MsG0580025023.01.T01	AT3G50990	57.616	302	78	2	383	634	41	342	1.30e-112	342
MsG0580025023.01.T01	AT1G44970	53.974	302	88	3	383	633	45	346	4.98e-98	304
MsG0580025023.01.T01	AT4G36490	39.811	530	136	15	10	368	14	531	2.75e-97	308
MsG0580025023.01.T01	AT2G18180	41.531	431	126	10	54	369	18	437	1.46e-90	288
MsG0580025023.01.T01	AT2G18180	41.531	431	126	10	54	369	45	464	1.72e-90	289
MsG0580025023.01.T01	AT2G18180	40.848	448	137	11	53	383	44	480	1.86e-90	289

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Find 136 sgRNAs with CRISPR-Local

Find 468 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTCTCAGGAATTCTT+TGG	0.264075	5:-12896889	MsG0580025023.01.T01:CDS
AAAGACTCTACAACCAAACT+GGG	0.636762	5:-12889426	MsG0580025023.01.T01:CDS
AAAGCTTCAGCACACATGCT+AGG	0.500325	5:-12896054	MsG0580025023.01.T01:CDS
AAAGGGTAGGCGAAGTAGTA+AGG	0.477923	5:-12896862	MsG0580025023.01.T01:CDS
AACAGATGAATACCTTCTTG+GGG	0.614618	5:+12894255	None:intergenic
AAGAACTCTTGGCCCCAAGA+AGG	0.585453	5:-12894267	MsG0580025023.01.T01:CDS
AAGAACTTATTGACCAACAA+GGG	0.649683	5:-12889268	MsG0580025023.01.T01:CDS
AAGCTTCAGCACACATGCTA+GGG	0.554708	5:-12896053	MsG0580025023.01.T01:CDS
AATCAGAAAACGCTGCAGAA+AGG	0.603094	5:-12889089	MsG0580025023.01.T01:CDS
AATGTTTCCCATCTTAACCA+TGG	0.593838	5:+12889138	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATTCTCAGGAATTCTT+TGG	-	Chr5:12889251-12889270	MsG0580025023.01.T01:CDS	30.0%
AAATACTATCATTTCCACCA+TGG	-	Chr5:12895401-12895420	MsG0580025023.01.T01:intron	30.0%
AAATATTGTTTCGATGTCAC+AGG	-	Chr5:12890057-12890076	MsG0580025023.01.T01:CDS	30.0%
AAATCTATAAAAGATCACCG+GGG	+	Chr5:12893894-12893913	None:intergenic	30.0%
AACGGAAGTTATGAAATAGT+GGG	+	Chr5:12894332-12894351	None:intergenic	30.0%
AACTTCTTAACTGATTGATG+AGG	-	Chr5:12890956-12890975	MsG0580025023.01.T01:intron	30.0%
AAGAACTTATTGACCAACAA+GGG	-	Chr5:12896872-12896891	MsG0580025023.01.T01:CDS	30.0%
AATATTCAATCCTTTCAGAC+TGG	-	Chr5:12896247-12896266	MsG0580025023.01.T01:CDS	30.0%
AATATTGTTTCGATGTCACA+GGG	-	Chr5:12890058-12890077	MsG0580025023.01.T01:CDS	30.0%
AATTAGGCTCAATCATTGTT+TGG	-	Chr5:12894923-12894942	MsG0580025023.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	12889078	12897084	12889078	ID=MsG0580025023.01;Name=MsG0580025023.01
Chr5	mRNA	12889078	12897084	12889078	ID=MsG0580025023.01.T01;Parent=MsG0580025023.01;Name=MsG0580025023.01.T01;_AED=0.50;_eAED=0.53;_QI=0\|0\|0\|1\|1\|1\|14\|0\|634
Chr5	exon	12897057	12897084	12897057	ID=MsG0580025023.01.T01:exon:20037;Parent=MsG0580025023.01.T01
Chr5	exon	12896799	12896942	12896799	ID=MsG0580025023.01.T01:exon:20036;Parent=MsG0580025023.01.T01
Chr5	exon	12896204	12896441	12896204	ID=MsG0580025023.01.T01:exon:20035;Parent=MsG0580025023.01.T01
Chr5	exon	12896008	12896083	12896008	ID=MsG0580025023.01.T01:exon:20034;Parent=MsG0580025023.01.T01
Chr5	exon	12895665	12895714	12895665	ID=MsG0580025023.01.T01:exon:20033;Parent=MsG0580025023.01.T01
Chr5	exon	12895426	12895525	12895426	ID=MsG0580025023.01.T01:exon:20032;Parent=MsG0580025023.01.T01
Chr5	exon	12895254	12895328	12895254	ID=MsG0580025023.01.T01:exon:20031;Parent=MsG0580025023.01.T01
Chr5	exon	12895105	12895172	12895105	ID=MsG0580025023.01.T01:exon:20030;Parent=MsG0580025023.01.T01
Chr5	exon	12894685	12894773	12894685	ID=MsG0580025023.01.T01:exon:20029;Parent=MsG0580025023.01.T01
Chr5	exon	12894240	12894424	12894240	ID=MsG0580025023.01.T01:exon:20028;Parent=MsG0580025023.01.T01
Chr5	exon	12893856	12893933	12893856	ID=MsG0580025023.01.T01:exon:20027;Parent=MsG0580025023.01.T01
Chr5	exon	12890457	12890609	12890457	ID=MsG0580025023.01.T01:exon:20026;Parent=MsG0580025023.01.T01
Chr5	exon	12889970	12890177	12889970	ID=MsG0580025023.01.T01:exon:20025;Parent=MsG0580025023.01.T01
Chr5	exon	12889078	12889490	12889078	ID=MsG0580025023.01.T01:exon:20024;Parent=MsG0580025023.01.T01
Chr5	CDS	12897057	12897084	12897057	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12896799	12896942	12896799	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12896204	12896441	12896204	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12896008	12896083	12896008	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12895665	12895714	12895665	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12895426	12895525	12895426	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12895254	12895328	12895254	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12895105	12895172	12895105	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12894685	12894773	12894685	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12894240	12894424	12894240	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12893856	12893933	12893856	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12890457	12890609	12890457	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12889970	12890177	12889970	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01
Chr5	CDS	12889078	12889490	12889078	ID=MsG0580025023.01.T01:cds;Parent=MsG0580025023.01.T01

Gene Sequence

>MsG0580025023.01.T01

ATGTCAGAACCTTTGGCTTCTGGGAATGGTTCTTTTAAGAAGAAAGCAATGAATGCCTCAAACATTCTCAGGAATTCTTTGGCAAAAAAGGGTAGGCGAAGTAGTAAGGTCATGTCTGTTGAAATTGAGGATGTTCATGATGCCGAGGAGTTGAAAGCTGTTGAAGAGTTCCGAATTGAATTCAAGGAAATTGATGAAGTATTGAAATATTATCCACAAGGACATCATGGTGTAGATAAAGAAGGACGACCTGTGTACATAGAAAGATTAGGACAAGTCGATGCGACCAAGATGATGCAAGTCACAACCATGGATCGCTATATCAAATACCATGTAAAGGAGTTTGAGAGGACATTCGATGTAAAGTTTGCGGCTTGTTCCATTGCTGCCAAGAAGCAACATCGATCAAAGGGCTTAAAAGCTTCAGCACACATGCTAGGGAACTTGTTACTCGCATTCAGAAGGTTGATGGTGACAACTATCCAGGTTCTTGGCAACAAGTATGATAGTAAGTTGCTTGAAGTAATTGACGAAAGTGAATTGCCTGAGTTTTTAGGAGGTACATGTAAATGTGCTGATGAAGGAGGATGTATGCGTTCCGATAAGGGCCCATGGAAAGATCCTGAAATACTGAGGTTGGTTGAGAGTGGGGCACACAAATGTTCAAAAAAATCTGAGTCTAATGTTGACGAAGAGAAAACAACTTCTGAGACCACACGACTTCCAAACGGAGGAAATCTCACCACCTCACAAGTTTTCTCCGATTCCGATTCTGAGAGCTTCCAAGCACGAGGATCCAATCCCGTAGATGATAAAAAAACTGCTCAGAAAAAAGTTGACGAGACAGACGCAATGGCAACCTCAGAAGAGTTTGTGACTGTCATGAAACGCATGACTGAGTTGGAACAGAAAATGACAACCATTAATCATCAGCCTGTTTTAATGCCACCTGAGAAGGAGGAAATGCTACATAATACTATTAATCGAGCTGATTTATTGGAAAAAGAACTCTTGGCCCCAAGAAGGTATTCATCTGTTAGAACAGCATATTTGGCTTTAGAGGATTCACTGGTTAAGCAAGAGGAGATTTCGGCTTATGTTGAGAAGAAAAAACAGAATAGAAGAAAGTGTAAAGTTGATGGTTACCTCTACCCTCAATTTTACGATGATTCGTGTCCCAAAGTTGAAGAGATTGTCAAGTCCGTAGTTGCTAAGGCTGTCGCTAAAGAACCCCGCATGGCTGCTTCCTTGCTGAGACTACATTTCCATGACTGTTTTGTCAAGGGTTGTGATGCATCAGTGTTGCTAGATAGCAGTGGAACAATCATCAGCGAAAAGAGGTCAAATCCAAACCGTAATTCAGCTAGAGGATTTGAAGTCATTGAAGAAATTAAATCCGCTGTAGAGAAAGAGTGTCCCCAGACAGTCTCTTGTGCTGATATTTTGACTCTAGCTGCTAGAGATTCAACTGTTCTTGTAAATATTTCCTCTTGTAGCCACACCATAGGAGATTCGCGGTGCACCAGCTTTAGACAAAGACTCTACAACCAAACTGGGAATGGCAAGTCAGATTTCACTCTTGACCAAAACTATGCAGCTCAATTGCGCACTCGGTGCCCAAGATCTGGTGGAGACCAAAATCTGTTTGTCCTAGACTTTGTCACACCGGGTAAATTCGACAACAACTACTACAAGAACTTATTGACCAACAAGGGTCTCCTAAGTTCTGATGAAATTCTGTTGACAAAGAATCAAGTATCTGCAGATTTAGTGAAAAAGTATGCAGAAAGCAATGATCTTTTCTTTGAACAATTTGCCAAGTCCATGGTTAAGATGGGAAACATTACTCCTTTAACAGGATCAAGGGGTGAAATCAGAAAACGCTGCAGAAAGGTTAACAACTGA

Protein sequence

>MsG0580025023.01.T01

MSEPLASGNGSFKKKAMNASNILRNSLAKKGRRSSKVMSVEIEDVHDAEELKAVEEFRIEFKEIDEVLKYYPQGHHGVDKEGRPVYIERLGQVDATKMMQVTTMDRYIKYHVKEFERTFDVKFAACSIAAKKQHRSKGLKASAHMLGNLLLAFRRLMVTTIQVLGNKYDSKLLEVIDESELPEFLGGTCKCADEGGCMRSDKGPWKDPEILRLVESGAHKCSKKSESNVDEEKTTSETTRLPNGGNLTTSQVFSDSDSESFQARGSNPVDDKKTAQKKVDETDAMATSEEFVTVMKRMTELEQKMTTINHQPVLMPPEKEEMLHNTINRADLLEKELLAPRRYSSVRTAYLALEDSLVKQEEISAYVEKKKQNRRKCKVDGYLYPQFYDDSCPKVEEIVKSVVAKAVAKEPRMAASLLRLHFHDCFVKGCDASVLLDSSGTIISEKRSNPNRNSARGFEVIEEIKSAVEKECPQTVSCADILTLAARDSTVLVNISSCSHTIGDSRCTSFRQRLYNQTGNGKSDFTLDQNYAAQLRTRCPRSGGDQNLFVLDFVTPGKFDNNYYKNLLTNKGLLSSDEILLTKNQVSADLVKKYAESNDLFFEQFAKSMVKMGNITPLTGSRGEIRKRCRKVNN*