AlfalfaGEDB | MsG0080048134.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048134.01

KEGG: -
GO: GO:0005506 GO:0016705 GO:0020037 GO:0055114
PFAM: PF00067

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048134.01.T01	KEH34483.1	95.172	145	7	0	1	145	205	349	1.12E-98	297
MsG0080048134.01.T02	KEH34483.1	95.556	90	4	0	1	90	260	349	2.16E-55	184

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048134.01.T01	Q50EK0	47.552	143	73	1	1	143	355	495	1.60E-42	149
MsG0080048134.01.T02	Q50EK0	46.591	88	45	1	1	88	410	495	5.68E-20	85.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048134.01.T01	A0A072UZE0	95.172	145	7	1	145	205	349	5.34e-99	297
MsG0080048134.01.T02	A0A072UY40	95.556	90	4	1	90	393	482	1.02e-54	186
MsG0080048134.01.T02	A0A072UZE0	95.556	90	4	1	90	260	349	1.03e-55	184

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048134.01	MsG0080048889.01	0.801991	6.933096e-49	3.357634e-46
MsG0080048134.01	MsG0080049046.01	0.810858	9.351553e-51	5.700812e-48
MsG0080048134.01	MsG0080049154.01	0.807620	4.625160e-50	2.588300e-47
MsG0080048134.01	MsG0180000029.01	0.800776	1.229835e-48	5.775589e-46
MsG0080048134.01	MsG0180000399.01	0.800297	1.539961e-48	7.145036e-46
MsG0080048134.01	MsG0180000495.01	0.800998	1.107618e-48	5.231677e-46
MsG0080048134.01	MsG0180000497.01	0.808695	2.729626e-50	1.571169e-47
MsG0080048134.01	MsG0180000723.01	0.810451	1.145458e-50	6.906841e-48
MsG0080048134.01	MsG0180000870.01	0.804058	2.591764e-49	1.323408e-46
MsG0080048134.01	MsG0180000985.01	0.812169	4.854410e-51	3.064214e-48

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048134.01.T01	MTR_3g467140	95.172	145	7	0	1	145	205	349	1.35e-102	297
MsG0080048134.01.T01	MTR_3g467140	95.172	145	7	0	1	145	338	482	2.46e-101	298
MsG0080048134.01.T01	MTR_3g467130	81.944	144	26	0	1	144	338	481	2.02e-86	260
MsG0080048134.01.T01	MTR_5g091760	47.857	140	70	1	1	140	341	477	4.34e-45	153
MsG0080048134.01.T01	MTR_5g092150	51.429	140	66	2	1	140	334	471	1.64e-43	149
MsG0080048134.01.T01	MTR_8g100135	45.000	140	75	1	1	140	340	477	1.48e-39	139
MsG0080048134.01.T01	MTR_8g089190	41.606	137	79	1	1	137	306	441	7.33e-35	125
MsG0080048134.01.T01	MTR_5g025610	40.000	140	81	1	1	140	327	463	1.37e-27	106
MsG0080048134.01.T01	MTR_8g072260	39.416	137	80	1	1	137	329	462	3.17e-25	99.8
MsG0080048134.01.T01	MTR_1g019410	40.441	136	78	1	1	136	333	465	4.33e-25	99.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048134.01.T01	AT2G42850	46.479	142	73	1	1	142	347	485	2.40e-45	154
MsG0080048134.01.T01	AT5G36130	46.099	141	73	2	1	140	1	139	3.47e-42	137
MsG0080048134.01.T01	AT5G36110	45.070	142	75	2	1	141	338	477	4.82e-38	134
MsG0080048134.01.T01	AT2G29090	41.007	139	79	1	1	139	251	386	3.39e-30	112
MsG0080048134.01.T01	AT5G45340	41.304	138	78	1	1	138	326	460	8.36e-30	112
MsG0080048134.01.T01	AT2G29090	41.007	139	79	1	1	139	346	481	1.55e-29	112
MsG0080048134.01.T01	AT2G29090	41.007	139	79	1	1	139	346	481	1.55e-29	112
MsG0080048134.01.T01	AT4G19230	40.000	145	82	2	1	143	326	467	4.26e-27	105
MsG0080048134.01.T01	AT5G45340	46.226	106	54	1	1	106	326	428	4.78e-25	99.4
MsG0080048134.01.T01	AT3G19270	40.299	134	75	2	1	133	330	459	2.17e-24	97.8

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Find 42 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTGTGAATGGTAGTTA+AGG	0.403773	contig216end:+34579	None:intergenic
AAGGGTGGGATTGGCTTGGT+TGG	0.421963	contig216end:+34667	None:intergenic
AATCCTAACTTGTAGACCTA+AGG	0.510863	contig216end:+34486	None:intergenic
AATGGAAGGTAAGTAAAGGG+TGG	0.648522	contig216end:+34652	None:intergenic
ACCAAATGGAAGGTAAGTAA+AGG	0.529962	contig216end:+34648	None:intergenic
ACTTACCTTCCATTTGGTGC+AGG	0.402034	contig216end:-34643	MsG0080048134.01.T01:CDS
AGGATGATCCCTCCCTTGTT+TGG	0.399326	contig216end:-34938	MsG0080048134.01.T01:CDS
AGGTAAGTAAAGGGTGGGAT+TGG	0.449267	contig216end:+34658	None:intergenic
AGTAAAGGGTGGGATTGGCT+TGG	0.491689	contig216end:+34663	None:intergenic
ATGATCCCTCCCTTGTTTGG+TGG	0.435807	contig216end:-34935	MsG0080048134.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGATATGACATCCAAAAA+GGG	-	contig216end:34553-34572	MsG0080048134.01.T01:CDS	30.0%
CATCTTCACAAAATTGTGAA+TGG	+	contig216end:34866-34885	None:intergenic	30.0%
GGCTAACAATAATGTCAAAT+TGG	-	contig216end:34647-34666	MsG0080048134.01.T01:CDS	30.0%
ACCAAATGGAAGGTAAGTAA+AGG	+	contig216end:34788-34807	None:intergenic	35.0%
CAAGGATATGACATCCAAAA+AGG	-	contig216end:34552-34571	MsG0080048134.01.T01:CDS	35.0%
CCAAATGGAAGGTAAGTAAA+GGG	+	contig216end:34787-34806	None:intergenic	35.0%
CTTAAAGATACTAGCTACCA+AGG	-	contig216end:34534-34553	MsG0080048134.01.T01:CDS	35.0%
TTCCTAAATCCACCAAACAA+GGG	+	contig216end:34510-34529	None:intergenic	35.0%
TTTCCTAAATCCACCAAACA+AGG	+	contig216end:34511-34530	None:intergenic	35.0%
AATGGAAGGTAAGTAAAGGG+TGG	+	contig216end:34784-34803	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
contig216end	gene	34459	34996	34459	ID=MsG0080048134.01;Name=MsG0080048134.01
contig216end	mRNA	34459	34996	34459	ID=MsG0080048134.01.T01;Parent=MsG0080048134.01;Name=MsG0080048134.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|1\|0.5\|2\|0\|145
contig216end	exon	34459	34773	34459	ID=MsG0080048134.01.T01:exon:6187;Parent=MsG0080048134.01.T01
contig216end	exon	34874	34996	34874	ID=MsG0080048134.01.T01:exon:6186;Parent=MsG0080048134.01.T01
contig216end	CDS	34874	34996	34874	ID=MsG0080048134.01.T01:cds;Parent=MsG0080048134.01.T01
contig216end	CDS	34459	34773	34459	ID=MsG0080048134.01.T01:cds;Parent=MsG0080048134.01.T01
contig216end	mRNA	34459	34731	34459	ID=MsG0080048134.01.T02;Parent=MsG0080048134.01;Name=MsG0080048134.01.T02;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|90
contig216end	exon	34459	34731	34459	ID=MsG0080048134.01.T02:exon:6188;Parent=MsG0080048134.01.T02
contig216end	CDS	34459	34731	34459	ID=MsG0080048134.01.T02:cds;Parent=MsG0080048134.01.T02

Gene Sequence

>MsG0080048134.01.T02

TTTGAGAATCCATACAAGTTTGAACCATCTCGTTTTGAAAATCCAACCAAGCCAATCCCACCCTTTACTTACCTTCCATTTGGTGCAGGGCTTCATAATTGCATAGGAAATGAGTTTGCCAGGGTTGAGACCTTAACTACCATTCACAATTTTGTGAAGATGTGTGAATGGTCTCAATTGAATCCAGAGGAAACTATTACTCGTCAACCTATGCCATATCCTTCCTTAGGTCTACAAGTTAGGATTAAACCAAGATGTAATATATCTTCTTAA

>MsG0080048134.01.T01

ATGAAATACACATGGAGAGTTGCTCAAGAATTGATGAGGATGATCCCTCCCTTGTTTGGTGGATTTAGGAAAGCACTTAAAGATACTAGCTACCAAGGATATGACATCCAAAAAGGGTGGCAGGTATATTGGGCATCAAGTGGAACACATGAGAACAAAGATATATTTGAGAATCCATACAAGTTTGAACCATCTCGTTTTGAAAATCCAACCAAGCCAATCCCACCCTTTACTTACCTTCCATTTGGTGCAGGGCTTCATAATTGCATAGGAAATGAGTTTGCCAGGGTTGAGACCTTAACTACCATTCACAATTTTGTGAAGATGTGTGAATGGTCTCAATTGAATCCAGAGGAAACTATTACTCGTCAACCTATGCCATATCCTTCCTTAGGTCTACAAGTTAGGATTAAACCAAGATGTAATATATCTTCTTAA

Protein sequence

>MsG0080048134.01.T02

FENPYKFEPSRFENPTKPIPPFTYLPFGAGLHNCIGNEFARVETLTTIHNFVKMCEWSQLNPEETITRQPMPYPSLGLQVRIKPRCNISS*

>MsG0080048134.01.T01

MKYTWRVAQELMRMIPPLFGGFRKALKDTSYQGYDIQKGWQVYWASSGTHENKDIFENPYKFEPSRFENPTKPIPPFTYLPFGAGLHNCIGNEFARVETLTTIHNFVKMCEWSQLNPEETITRQPMPYPSLGLQVRIKPRCNISS*