AlfalfaGEDB | MsG0580025861.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025861.01

KEGG: -
GO: -
PFAM: PF06880

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025861.01.T01	XP_003613362.1	92.945	326	23	0	345	670	61	386	0	627
MsG0580025861.01.T01	XP_003613362.1	71.217	337	68	3	1	310	1	335	5.19E-157	468

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025861.01.T01	G7K475	92.945	326	23	0	345	670	61	386	0.0	627

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280008117.01	MsG0580025861.01	0.815824	7.582424e-52	5.283229e-49
MsG0380017386.01	MsG0580025861.01	0.803258	3.798922e-49	1.900222e-46
MsG0580025180.01	MsG0580025861.01	0.802081	6.643573e-49	3.224885e-46
MsG0580025860.01	MsG0580025861.01	0.867410	1.397102e-65	4.873508e-62
MsG0580025861.01	MsG0680031429.01	0.827605	1.426869e-54	1.384057e-51

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025861.01.T01	MTR_5g035690	92.945	326	23	0	345	670	61	386	0.0	627
MsG0580025861.01.T01	MTR_5g035690	71.217	337	68	3	1	310	1	335	6.29e-161	468
MsG0580025861.01.T01	MTR_5g035665	92.532	308	23	0	363	670	72	379	0.0	590
MsG0580025861.01.T01	MTR_5g035665	72.424	330	69	2	1	310	1	328	2.88e-166	481
MsG0580025861.01.T01	MTR_5g035750	87.926	323	38	1	349	670	65	387	0.0	582
MsG0580025861.01.T01	MTR_5g035750	69.027	339	73	3	1	310	1	336	1.52e-155	454
MsG0580025861.01.T01	MTR_5g035760	83.891	329	34	1	1	310	1	329	0.0	569
MsG0580025861.01.T01	MTR_5g035760	72.674	344	85	3	336	670	37	380	4.32e-172	496
MsG0580025861.01.T01	MTR_5g035670	82.759	319	52	2	354	670	64	381	0.0	533
MsG0580025861.01.T01	MTR_5g035670	69.091	330	82	1	1	310	1	330	3.11e-152	446

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025861.01.T01	AT1G13480	41.257	366	181	13	319	670	42	387	4.61e-73	241
MsG0580025861.01.T01	AT1G13480	38.484	343	166	10	1	308	1	333	8.27e-57	197
MsG0580025861.01.T01	AT1G13530	40.489	368	179	14	319	670	42	385	5.48e-73	241
MsG0580025861.01.T01	AT1G13530	41.813	342	158	9	1	311	1	332	4.56e-60	206
MsG0580025861.01.T01	AT1G13520	44.012	334	160	12	354	670	64	387	3.27e-72	239
MsG0580025861.01.T01	AT1G13520	38.596	342	166	10	1	307	1	333	8.72e-56	195
MsG0580025861.01.T01	AT1G13510	40.054	367	194	11	314	670	30	380	1.30e-71	237
MsG0580025861.01.T01	AT1G13510	40.000	340	162	11	1	311	1	327	3.69e-63	214
MsG0580025861.01.T01	AT1G13470	46.057	317	152	10	363	670	72	378	4.05e-71	236
MsG0580025861.01.T01	AT1G13470	40.060	332	154	11	1	301	1	318	1.09e-54	192

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Find 164 sgRNAs with CRISPR-Local

Find 489 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCACAATAAAAGCATG+TGG	0.665724	5:-25312559	None:intergenic
AAAATTGGAAAGTTGAAATT+TGG	0.232410	5:-25312801	None:intergenic
AAACAGTCACAAACTACAAG+TGG	0.602720	5:-25310921	None:intergenic
AAACATAAATTCAAAGGAAG+AGG	0.508991	5:+25307297	MsG0580025861.01.T01:CDS
AAAGATTTGGTTGGATTTCT+GGG	0.321762	5:+25307932	MsG0580025861.01.T01:CDS
AAAGTTGAAATTTGGTAGCT+TGG	0.373273	5:-25312793	None:intergenic
AAATGGGAACAAAGAAGGTT+TGG	0.394970	5:+25313146	MsG0580025861.01.T01:CDS
AAATGGGAACAAGAAAGATT+TGG	0.391384	5:+25307919	MsG0580025861.01.T01:CDS
AACCAGTCGTTGTTGGAAAA+TGG	0.314365	5:+25307605	MsG0580025861.01.T01:CDS
AACTCCTTGTGGTTTATTAA+GGG	0.361842	5:+25307456	MsG0580025861.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAGAATGGATAGAAGAT+TGG	+	Chr5:25308053-25308072	MsG0580025861.01.T01:intron	30.0%
AAATGGGAACAAGAAAGATT+TGG	+	Chr5:25307919-25307938	MsG0580025861.01.T01:CDS	30.0%
AACTCCTTGTGGTTTATTAA+GGG	+	Chr5:25307456-25307475	MsG0580025861.01.T01:CDS	30.0%
AATCTCTTGGAAGACAAAAA+AGG	-	Chr5:25311593-25311612	None:intergenic	30.0%
AATGACATTAAAGGAACAGA+TGG	+	Chr5:25307657-25307676	MsG0580025861.01.T01:CDS	30.0%
AATTATAAGCCTAGTGGTAT+TGG	+	Chr5:25311129-25311148	MsG0580025861.01.T01:intron	30.0%
ACAAAACAACCAAATGTAGT+TGG	-	Chr5:25309945-25309964	None:intergenic	30.0%
ACAAAAGTTGAAGAATTTGG+TGG	+	Chr5:25313203-25313222	MsG0580025861.01.T01:CDS	30.0%
ACTATGTTATAAGAAGGAAG+GGG	+	Chr5:25312285-25312304	MsG0580025861.01.T01:CDS	30.0%
AGAAAATGTTATGTTCCTTC+CGG	+	Chr5:25306882-25306901	MsG0580025861.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	25306664	25313355	25306664	ID=MsG0580025861.01;Name=MsG0580025861.01
Chr5	mRNA	25306664	25313355	25306664	ID=MsG0580025861.01.T01;Parent=MsG0580025861.01;Name=MsG0580025861.01.T01;_AED=0.41;_eAED=0.42;_QI=0\|0\|0\|0.83\|1\|1\|6\|0\|670
Chr5	exon	25306664	25306811	25306664	ID=MsG0580025861.01.T01:exon:4099;Parent=MsG0580025861.01.T01
Chr5	exon	25306869	25306968	25306869	ID=MsG0580025861.01.T01:exon:4100;Parent=MsG0580025861.01.T01
Chr5	exon	25307291	25307966	25307291	ID=MsG0580025861.01.T01:exon:4101;Parent=MsG0580025861.01.T01
Chr5	exon	25310909	25310961	25310909	ID=MsG0580025861.01.T01:exon:4102;Parent=MsG0580025861.01.T01
Chr5	exon	25312115	25312318	25312115	ID=MsG0580025861.01.T01:exon:4103;Parent=MsG0580025861.01.T01
Chr5	exon	25312524	25313355	25312524	ID=MsG0580025861.01.T01:exon:4104;Parent=MsG0580025861.01.T01
Chr5	CDS	25306664	25306811	25306664	ID=MsG0580025861.01.T01:cds;Parent=MsG0580025861.01.T01
Chr5	CDS	25306869	25306968	25306869	ID=MsG0580025861.01.T01:cds;Parent=MsG0580025861.01.T01
Chr5	CDS	25307291	25307966	25307291	ID=MsG0580025861.01.T01:cds;Parent=MsG0580025861.01.T01
Chr5	CDS	25310909	25310961	25310909	ID=MsG0580025861.01.T01:cds;Parent=MsG0580025861.01.T01
Chr5	CDS	25312115	25312318	25312115	ID=MsG0580025861.01.T01:cds;Parent=MsG0580025861.01.T01
Chr5	CDS	25312524	25313355	25312524	ID=MsG0580025861.01.T01:cds;Parent=MsG0580025861.01.T01

Gene Sequence

>MsG0580025861.01.T01

ATGTATGCAGCAAGACTCCTTTCCATGTACAAAAGGAACCCTAGTGCTCTTTCAGATCCACCACCATCAGGTCCAAATTCAGGTTATCTTGTAATATTGGATGAAGAAGCTCAAACTTACTCTTTCTTTGGTTTGTGGAAGGACATTGGTATATACCAAAGAGAAAATGTTATGTTCCTTCCGGTATTGAATCAACCATTGTCTTCGAACCGTTATCATGTCATAAGAAGGAAGGGGAAGAATCAAGGCCAAGCAAACATAAATTCAAAGGAAGAGGACATTAGCACTAGTGTATGCTGCCATTTTGGCCATGATATTAAAACAAGTCCCTTGGAGCCCTTCAATCATTATCAACAAATGGAAATAAGCAAGAAACGTTTTGGTTTTCATGCCGAGTCGGTTGCCTTGGATGGAACTCCTTGTGGTTTATTAAGGGAAAAAGTGTGGACAGTTTTCGCTACCACTCCTACAAATTACCATTTGAGTGAAGCCTTGGCTTCAAATGATTCCTTGCGTTCTAAGCTACCAGATTTCAACTTTCCATTGTCCAATGATCGTTCTGAACCAGTCGTTGTTGGAAAATGGTATTGTCCTTTTATGTTTGTGAAGGAAGGAATGACATTAAAGGAACAGATGGAAATATCAATGTTCTATGAGTTGACTCTCGAGCAAAGATGGGAGAAGATATTTTCAAAAGAAAATGTGAACAGTGAAGAGAGTGATGTATTTGTAGATGTTGTTATTCAGACAGAAGATGCTAAGGTTGAAGGAAAGGATGTTGTGTGGGATGAAAATAGATTGGTTGATGGAGTTTCGTGGTTTAAGAGTGTTGAAGAGAAAAGTGTTGGATTGAGTTTGGAAGTTGTTAAAGGAATGAAATGGGAACAAGAAAGATTTGGTTGGATTTCTGGGAATGGAAGACAAATTAAATCTAGACCACTTGTAGTTTGTGACTGTTTTGGTTATGACTTTCACTGTCAAACTGGTTATTCATTTGGTGTAAACAGTCAAACTGGTTATTCATTTGGTGTAAACCGTCAAACTGGTTATTCATCTGGTGTAAACAGTCAAACAGGACATGCAAACACCTACAGTGAAGAAGCTATGTTCATTCCAGTATTGAATCAACCATTGTCTTCGAATCGCTACTATGTTATAAGAAGGAAGGGGAAGTATCAGGGCCAAGCTAATACAAGTTCAAAGGAAGAGGACATGACCACATGCTTTTATTGTGGTTTTGTCAATGATGTTAAACCAATAGCTTTGGAGCCCTTCAATGATTATCAACAGGTAGAAATAATCAAAAAAAGTTATGGTTTTCACGCAAAATCCGTTGCCTCTGATGGAATTCCTCCTGGGTTATTGAGGGAAAAAGGGTGGACACTTTATGCTAGCACTCCCCGCAACTACCATTTAAGCCAAGCTTTAGGCTCAAATGATTCCTTGCGTTCCAAGCTACCAAATTTCAACTTTCCAATTTTCAATGATTGTTCCGAATCAGTGGTTGTTGGAAAGTGGTATTGTCCATTTATGTTTGTGAAGGAAGGAATGAATTTAAAAGAGCAGATGAAGATGTCAGTGTTCTATGAGCTAACACTTGAGCAAAGATGGGAGAAGATTTTTTCAAAGGAAAATAGTGGAGAAGGTGGTGTGCTTGTAGATGTTATTATTCAAACAGAAGTTGCTAAGGTTGAAGGGAAGGATGCAGTTTGGGATGAAAATAGATTGGTTGATGGAGTTTTGTGGTTTAAGAGTGTTGATGAGAAAAGTGTTGGATTGAGTTTGGAAGTTGTTGAGGCAATGAAATGGGAACAAAGAAGGTTTGGATGGAATGCTGGGAAGGGAAGACAAGTGAGAGTAACAAAAGTTGAAGAATTTGGTGGAACAAACAAATGGAAGAAATTTAGTAGTTATGTGTTGGTTGAAAGTTATTCTTTGAGGAGAATGGATACAAAATTGGTTCTTACATATGATTATAGACACTCACATCAAATTAGAAGCAAATGGGAATGA

Protein sequence

>MsG0580025861.01.T01

MYAARLLSMYKRNPSALSDPPPSGPNSGYLVILDEEAQTYSFFGLWKDIGIYQRENVMFLPVLNQPLSSNRYHVIRRKGKNQGQANINSKEEDISTSVCCHFGHDIKTSPLEPFNHYQQMEISKKRFGFHAESVALDGTPCGLLREKVWTVFATTPTNYHLSEALASNDSLRSKLPDFNFPLSNDRSEPVVVGKWYCPFMFVKEGMTLKEQMEISMFYELTLEQRWEKIFSKENVNSEESDVFVDVVIQTEDAKVEGKDVVWDENRLVDGVSWFKSVEEKSVGLSLEVVKGMKWEQERFGWISGNGRQIKSRPLVVCDCFGYDFHCQTGYSFGVNSQTGYSFGVNRQTGYSSGVNSQTGHANTYSEEAMFIPVLNQPLSSNRYYVIRRKGKYQGQANTSSKEEDMTTCFYCGFVNDVKPIALEPFNDYQQVEIIKKSYGFHAKSVASDGIPPGLLREKGWTLYASTPRNYHLSQALGSNDSLRSKLPNFNFPIFNDCSESVVVGKWYCPFMFVKEGMNLKEQMKMSVFYELTLEQRWEKIFSKENSGEGGVLVDVIIQTEVAKVEGKDAVWDENRLVDGVLWFKSVDEKSVGLSLEVVEAMKWEQRRFGWNAGKGRQVRVTKVEEFGGTNKWKKFSSYVLVESYSLRRMDTKLVLTYDYRHSHQIRSKWE*