Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017386.01.T01 | XP_003603333.2 | 99.306 | 432 | 3 | 0 | 1 | 432 | 1 | 432 | 0 | 861 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048345.01 | MsG0380017386.01 | 0.819928 | 8.973751e-53 | 7.001009e-50 |
| MsG0080048347.01 | MsG0380017386.01 | 0.814276 | 1.673420e-51 | 1.117932e-48 |
| MsG0080048352.01 | MsG0380017386.01 | 0.824765 | 6.758449e-54 | 6.043655e-51 |
| MsG0080048967.01 | MsG0380017386.01 | 0.804399 | 2.201340e-49 | 1.133836e-46 |
| MsG0180000437.01 | MsG0380017386.01 | 0.820197 | 7.788822e-53 | 6.122244e-50 |
| MsG0180000930.01 | MsG0380017386.01 | 0.801236 | 9.901740e-49 | 4.704851e-46 |
| MsG0180001050.01 | MsG0380017386.01 | 0.867387 | 1.421067e-65 | 4.953344e-62 |
| MsG0180003240.01 | MsG0380017386.01 | 0.862601 | 4.515669e-64 | 1.329889e-60 |
| MsG0180004203.01 | MsG0380017386.01 | 0.833573 | 4.935160e-56 | 5.706249e-53 |
| MsG0180004562.01 | MsG0380017386.01 | 0.803087 | 4.119930e-49 | 2.051695e-46 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017386.01.T01 | MTR_3g106430 | 99.306 | 432 | 3 | 0 | 1 | 432 | 1 | 432 | 0.0 | 861 |
| MsG0380017386.01.T01 | MTR_3g106480 | 92.593 | 432 | 32 | 0 | 1 | 432 | 1 | 432 | 0.0 | 808 |
| MsG0380017386.01.T01 | MTR_3g106485 | 92.130 | 432 | 34 | 0 | 1 | 432 | 1 | 432 | 0.0 | 780 |
| MsG0380017386.01.T01 | MTR_3g106420 | 92.130 | 432 | 34 | 0 | 1 | 432 | 3 | 434 | 0.0 | 777 |
| MsG0380017386.01.T01 | MTR_1g083190 | 92.593 | 432 | 32 | 0 | 1 | 432 | 3 | 434 | 0.0 | 774 |
| MsG0380017386.01.T01 | MTR_1g073400 | 92.757 | 428 | 31 | 0 | 1 | 428 | 1 | 428 | 0.0 | 759 |
| MsG0380017386.01.T01 | MTR_1g071130 | 86.806 | 432 | 31 | 1 | 1 | 432 | 1 | 406 | 0.0 | 756 |
| MsG0380017386.01.T01 | MTR_1g115145 | 89.145 | 433 | 40 | 3 | 1 | 432 | 1 | 427 | 0.0 | 752 |
| MsG0380017386.01.T01 | MTR_3g106470 | 81.713 | 432 | 23 | 2 | 1 | 432 | 32 | 407 | 0.0 | 658 |
| MsG0380017386.01.T01 | MTR_8g063830 | 80.831 | 433 | 42 | 5 | 1 | 432 | 1 | 393 | 0.0 | 613 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017386.01.T01 | AT5G25250 | 76.761 | 426 | 88 | 1 | 1 | 426 | 1 | 415 | 0.0 | 671 |
| MsG0380017386.01.T01 | AT5G25260 | 75.991 | 429 | 92 | 1 | 1 | 429 | 1 | 418 | 0.0 | 667 |
| MsG0380017386.01.T01 | AT5G64870 | 72.055 | 433 | 110 | 1 | 2 | 434 | 3 | 424 | 0.0 | 600 |
Find 106 sgRNAs with CRISPR-Local
Find 141 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACAACTACACAGCTGTAA+GGG | 0.585417 | 3:-95694338 | MsG0380017386.01.T01:CDS |
| AAACAGTTAGTTGATGTGCC+AGG | 0.562976 | 3:-95695853 | MsG0380017386.01.T01:CDS |
| AAAGGTTATTGCGATGCAGA+GGG | 0.625617 | 3:-95695674 | MsG0380017386.01.T01:CDS |
| AACAAGTTCCTTAACATGAT+TGG | 0.309318 | 3:+95696029 | None:intergenic |
| AACACTTGGGAATGCTCAAG+GGG | 0.627744 | 3:-95694392 | MsG0380017386.01.T01:CDS |
| AAGAAAGCATGGATTCTACC+CGG | 0.664070 | 3:-95696228 | MsG0380017386.01.T01:CDS |
| AAGACAGCGGGGAGAACAAA+AGG | 0.470694 | 3:+95696129 | None:intergenic |
| AAGACGGAGTAGGATTGACC+GGG | 0.657113 | 3:+95696210 | None:intergenic |
| AAGATTAGCATTTGGACTAA+TGG | 0.384982 | 3:-95694238 | MsG0380017386.01.T01:CDS |
| ACAAGGAGGGAATTAAAGTG+AGG | 0.693697 | 3:-95695639 | MsG0380017386.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATTGATGCAGAGACAA+AGG | - | Chr3:95694821-95694840 | MsG0380017386.01.T01:intron | 30.0% | |
| AACAAGTTCCTTAACATGAT+TGG | + | Chr3:95694487-95694506 | None:intergenic | 30.0% | |
| AAGATTAGCATTTGGACTAA+TGG | - | Chr3:95696275-95696294 | MsG0380017386.01.T01:CDS | 30.0% | |
| AGAGCTATATGCAAAGAAAA+AGG | - | Chr3:95696082-95696101 | MsG0380017386.01.T01:CDS | 30.0% | |
| ATTGAAATTCTGGATGGTAT+AGG | - | Chr3:95695923-95695942 | MsG0380017386.01.T01:CDS | 30.0% | |
| CAACTTGAACTAAACCAATT+CGG | - | Chr3:95694612-95694631 | MsG0380017386.01.T01:intron | 30.0% | |
| TAAACTCTCCAATCATGTTA+AGG | - | Chr3:95694476-95694495 | MsG0380017386.01.T01:CDS | 30.0% | |
| TACTATAATGAGATCACAGT+TGG | - | Chr3:95695722-95695741 | MsG0380017386.01.T01:CDS | 30.0% | |
| TATGAGAAATATTCATGACC+TGG | + | Chr3:95694681-95694700 | None:intergenic | 30.0% | |
| TTAGTCCAAATGCTAATCTT+AGG | + | Chr3:95696275-95696294 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 95694213 | 95696322 | 95694213 | ID=MsG0380017386.01;Name=MsG0380017386.01 |
| Chr3 | mRNA | 95694213 | 95696322 | 95694213 | ID=MsG0380017386.01.T01;Parent=MsG0380017386.01;Name=MsG0380017386.01.T01;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|434 |
| Chr3 | exon | 95695396 | 95696322 | 95695396 | ID=MsG0380017386.01.T01:exon:13010;Parent=MsG0380017386.01.T01 |
| Chr3 | exon | 95694213 | 95694590 | 95694213 | ID=MsG0380017386.01.T01:exon:13009;Parent=MsG0380017386.01.T01 |
| Chr3 | CDS | 95695396 | 95696322 | 95695396 | ID=MsG0380017386.01.T01:cds;Parent=MsG0380017386.01.T01 |
| Chr3 | CDS | 95694213 | 95694590 | 95694213 | ID=MsG0380017386.01.T01:cds;Parent=MsG0380017386.01.T01 |
| Gene Sequence |
>MsG0380017386.01.T01 ATGTACAAGGTAGCAAAAGCATCACAATACCTTGTGATAACTGGAATCGGAATCAAAGACATAAAGCTTGCAAAGAAAGCATGGATTCTACCCGGTCAATCCTACTCCGTCTTCGACCTCTCACCTGTAAATTACACCTTTGAAGTTCAAGCAATGAGCGCAGAGAAACTTCCTTTTGTTCTCCCCGCTGTCTTCACTATCGGTCCTCGTGTGGATGATAAGGAAAGCCTTCTCAAATACGCCAAACTAATTTCCCCTCATGATAAACTCTCCAATCATGTTAAGGAACTTGTTCAAGGAATCATTGAAGGCGAGACACGTGTCCTCGCTGCTTCAATGACAATGGAAGAGGTTTTCAGAGGAACAAAAGAGTTTAAACAAGAAGTATTTGGGAAAGTTCAACTTGAACTAAACCAATTCGGTTTATTGATTTACAATGCTAATGTTAAACAGTTAGTTGATGTGCCAGGTCATGAATATTTCTCATATTTGGGCCAAAAGACACAAATGGAAGCAGCGAATCAAGCAAGGGTTGATGTATCAGAAGCTAAGATGAAAGGTGAGATTGGTTCGAAATTAAGGGAAGGACAAACACTACAAAATGCAGCAAAAATTGATGCAGAGACAAAGGTTATTGCGATGCAGAGGGCTGGAGAGGGCGACAAGGAGGGAATTAAAGTGAGGACTGAAGTGAAGGTTTTTGAGAATCAGAGAGAAGCTGAAGTGGCTGAGGCTAATTCTGAATTGGCTAAGAAGAAAGCGGCTTGGACTAAGGCGGCTCAAGTGGCCGAAGTTGAGGCTGCTAAAGCCGTAGCTCTAAGGGATGCTGAGTTACAAGGTGAAGTTGAGAGGATGAATGCATTGACTACAACTGAGAAGCTTAAAGCAGAGTTCCTTAGCAAAGCTAGCGTTCAATATGAAACCAAGGTACAAGAAGCAAACTGGGAGCTGTACAAGAAACAAAAGGAGGCGGAAGCGATTTTGTATGAGAAGAAGGCAGAGGCAGAAGCACAGAAAGCATTAGCAGACGCAACATTTTATGCTCGCACGCAAGCAGCTGAAGCAGAGCTATATGCAAAGAAAAAGGAGGCAGAGGGAATTGTAACACTTGGGAATGCTCAAGGGGTGTATCTAAGTGCACTTCTGAATGCACTTGGAAACAACTACACAGCTGTAAGGGATTACTTAATGATAAATGGTGGCATGTTTCAAGAGATTGCTAAGATTAATGCCGAGGCGGTACGCGGACTTGAGCCTAAGATTAGCATTTGGACTAATGGTGGTGATAATAGGGGGAATAACTGA |
| Protein sequence |
>MsG0380017386.01.T01 MYKVAKASQYLVITGIGIKDIKLAKKAWILPGQSYSVFDLSPVNYTFEVQAMSAEKLPFVLPAVFTIGPRVDDKESLLKYAKLISPHDKLSNHVKELVQGIIEGETRVLAASMTMEEVFRGTKEFKQEVFGKVQLELNQFGLLIYNANVKQLVDVPGHEYFSYLGQKTQMEAANQARVDVSEAKMKGEIGSKLREGQTLQNAAKIDAETKVIAMQRAGEGDKEGIKVRTEVKVFENQREAEVAEANSELAKKKAAWTKAAQVAEVEAAKAVALRDAELQGEVERMNALTTTEKLKAEFLSKASVQYETKVQEANWELYKKQKEAEAILYEKKAEAEAQKALADATFYARTQAAEAELYAKKKEAEGIVTLGNAQGVYLSALLNALGNNYTAVRDYLMINGGMFQEIAKINAEAVRGLEPKISIWTNGGDNRGNN* |
