Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008117.01.T01 | XP_003594889.2 | 97.046 | 237 | 6 | 1 | 1 | 236 | 1 | 237 | 8.89E-165 | 464 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008117.01.T01 | G7INF7 | 97.046 | 237 | 6 | 1 | 1 | 236 | 1 | 237 | 4.25e-165 | 464 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001050.01 | MsG0280008117.01 | 0.825623 | 4.236366e-54 | 3.881870e-51 |
| MsG0180003240.01 | MsG0280008117.01 | 0.851546 | 8.269692e-61 | 1.679692e-57 |
| MsG0180005697.01 | MsG0280008117.01 | 0.836343 | 9.895446e-57 | 1.243592e-53 |
| MsG0280008117.01 | MsG0280008258.01 | 0.830686 | 2.554112e-55 | 2.710675e-52 |
| MsG0280008117.01 | MsG0280011041.01 | 0.823039 | 1.716907e-53 | 1.461835e-50 |
| MsG0280008117.01 | MsG0280011042.01 | 0.807322 | 5.350164e-50 | 2.971035e-47 |
| MsG0280008117.01 | MsG0280011053.01 | 0.834492 | 2.904892e-56 | 3.453042e-53 |
| MsG0280008117.01 | MsG0380011613.01 | 0.806657 | 7.395630e-50 | 4.036417e-47 |
| MsG0280008117.01 | MsG0380014920.01 | 0.821812 | 3.308181e-53 | 2.720133e-50 |
| MsG0280008117.01 | MsG0380016643.01 | 0.828437 | 8.994969e-55 | 8.940653e-52 |
| MsG0280008117.01 | MsG0380017122.01 | 0.840063 | 1.089711e-57 | 1.534472e-54 |
| MsG0280008117.01 | MsG0380017386.01 | 0.823720 | 1.189833e-53 | 1.032833e-50 |
| MsG0280008117.01 | MsG0480019894.01 | 0.803567 | 3.278135e-49 | 1.652908e-46 |
| MsG0280008117.01 | MsG0480020219.01 | 0.838321 | 3.082709e-57 | 4.113801e-54 |
| MsG0280008117.01 | MsG0580025180.01 | 0.813752 | 2.183509e-51 | 1.438076e-48 |
| MsG0280008117.01 | MsG0580025683.01 | 0.830520 | 2.803626e-55 | 2.960859e-52 |
| MsG0280008117.01 | MsG0580025861.01 | 0.815824 | 7.582424e-52 | 5.283229e-49 |
| MsG0280008117.01 | MsG0580025876.01 | 0.806458 | 8.147254e-50 | 4.424458e-47 |
| MsG0280008117.01 | MsG0580027227.01 | 0.820135 | 8.049075e-53 | 6.316199e-50 |
| MsG0280008117.01 | MsG0580029555.01 | 0.851784 | 7.083372e-61 | 1.449618e-57 |
| MsG0280008117.01 | MsG0680031429.01 | 0.856411 | 3.275338e-62 | 7.810941e-59 |
| MsG0280008117.01 | MsG0680034852.01 | 0.800378 | 1.482487e-48 | 6.892535e-46 |
| MsG0280008117.01 | MsG0680034919.01 | 0.816565 | 5.179760e-52 | 3.683001e-49 |
| MsG0280008117.01 | MsG0680035496.01 | 0.827733 | 1.329012e-54 | 1.294031e-51 |
| MsG0280008117.01 | MsG0780036946.01 | 0.823632 | 1.247371e-53 | 1.080002e-50 |
| MsG0280008117.01 | MsG0780039334.01 | 0.843103 | 1.721398e-58 | 2.664724e-55 |
| MsG0280008117.01 | MsG0780039968.01 | 0.807050 | 6.108664e-50 | 3.368210e-47 |
| MsG0280008117.01 | MsG0880044669.01 | 0.808264 | 3.372706e-50 | 1.919770e-47 |
| MsG0280008117.01 | MsG0880047747.01 | 0.810982 | 8.791650e-51 | 5.377616e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008117.01.T01 | MTR_2g035850 | 97.046 | 237 | 6 | 1 | 1 | 236 | 1 | 237 | 1.08e-168 | 464 |
| MsG0280008117.01.T01 | MTR_4g094698 | 50.000 | 134 | 44 | 6 | 1 | 131 | 1 | 114 | 2.47e-30 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACCTTGTTGAAAGTTTA+AGG | 0.076288 | 2:-25623314 | None:intergenic |
| GCTCTAGATCTTCTTCTAAT+TGG | 0.209397 | 2:-25623157 | None:intergenic |
| TTGCACAAGTTGTCTAAAAT+TGG | 0.266497 | 2:-25623221 | None:intergenic |
| GATAGTATTGCATCCAATAT+TGG | 0.285397 | 2:-25622967 | None:intergenic |
| TTGGTTGTGGATAATGATTT+TGG | 0.308541 | 2:+25623577 | MsG0280008117.01.T01:CDS |
| CTCAAAATCATAGTCTCTAA+TGG | 0.318292 | 2:-25623070 | None:intergenic |
| TGTTGAAAGTTTAAGGTAAT+AGG | 0.323637 | 2:-25623307 | None:intergenic |
| CTGCTTCTACTGACCAATAT+TGG | 0.333962 | 2:+25622954 | MsG0280008117.01.T01:CDS |
| AGATCTTCTTCTAATTGGTT+TGG | 0.337371 | 2:-25623152 | None:intergenic |
| TGCTCCTTGTTCCATGGAAA+TGG | 0.342079 | 2:-25623430 | None:intergenic |
| CTTGGATGTTTCTGATGGTT+TGG | 0.350629 | 2:+25623558 | MsG0280008117.01.T01:CDS |
| TCATCACATAAAAGTATCAT+TGG | 0.393436 | 2:-25623629 | None:intergenic |
| TTGGATATATTACCACCCTT+TGG | 0.400197 | 2:-25623133 | None:intergenic |
| GTGTTTGTGTATGAATTTGT+TGG | 0.407347 | 2:-25623094 | None:intergenic |
| TACCACCCTTTGGTGTCAAT+TGG | 0.410144 | 2:-25623123 | None:intergenic |
| ATCATAGTCTCTAATGGAAT+AGG | 0.414200 | 2:-25623064 | None:intergenic |
| GACAACGAGTTACTCGAATT+CGG | 0.437705 | 2:-25623523 | None:intergenic |
| TTTGCATTGAGTAGTGTTGT+TGG | 0.438305 | 2:-25623193 | None:intergenic |
| TGTTTCTGATGGTTTGGTTG+TGG | 0.449182 | 2:+25623564 | MsG0280008117.01.T01:CDS |
| GTTGTCAAGGCCTAGTAACT+TGG | 0.455240 | 2:+25623540 | MsG0280008117.01.T01:CDS |
| TGTTTGTGTATGAATTTGTT+GGG | 0.464118 | 2:-25623093 | None:intergenic |
| TGAATTTGCTCCTTGTTCCA+TGG | 0.471507 | 2:-25623436 | None:intergenic |
| AGTAACTTGGATGTTTCTGA+TGG | 0.501901 | 2:+25623553 | MsG0280008117.01.T01:CDS |
| CTTTGGTGTCAATTGGTCAC+TGG | 0.505907 | 2:-25623116 | None:intergenic |
| TCGCCGCTAGGTGTTCATAA+AGG | 0.509070 | 2:+25623283 | MsG0280008117.01.T01:CDS |
| TTGTCCTCGTCGTCGCCGCT+AGG | 0.509736 | 2:+25623271 | MsG0280008117.01.T01:CDS |
| ATACTATCAACAAACTCTCA+TGG | 0.509978 | 2:+25622982 | MsG0280008117.01.T01:CDS |
| TCAGAAACATCCAAGTTACT+AGG | 0.537297 | 2:-25623550 | None:intergenic |
| AGTGACCAATTGACACCAAA+GGG | 0.544491 | 2:+25623118 | MsG0280008117.01.T01:CDS |
| CAACTTGTGCAACAATTCAC+AGG | 0.545584 | 2:+25623232 | MsG0280008117.01.T01:CDS |
| AGGAGCAAATTCAAGCGCCT+CGG | 0.584501 | 2:+25623446 | MsG0280008117.01.T01:CDS |
| GACGAGGACAAAGTTGTACT+AGG | 0.587439 | 2:-25623259 | None:intergenic |
| TAACCACTCTGCTGTTGCCG+AGG | 0.590379 | 2:-25623463 | None:intergenic |
| TTGGTCAGTAGAAGCAGTCA+TGG | 0.595873 | 2:-25622948 | None:intergenic |
| CAGTGACCAATTGACACCAA+AGG | 0.605207 | 2:+25623117 | MsG0280008117.01.T01:CDS |
| AAGTGACTAAGCCATTTCCA+TGG | 0.609099 | 2:+25623419 | MsG0280008117.01.T01:CDS |
| ATTCGAGTAACTCGTTGTCA+AGG | 0.619347 | 2:+25623527 | MsG0280008117.01.T01:CDS |
| TAAGCCATTTCCATGGAACA+AGG | 0.637693 | 2:+25623426 | MsG0280008117.01.T01:CDS |
| AACACCTAGCGGCGACGACG+AGG | 0.640819 | 2:-25623275 | None:intergenic |
| TTCAATCCATAACACATGAG+TGG | 0.650240 | 2:+25622923 | None:intergenic |
| ATTACCTTAAACTTTCAACA+AGG | 0.690320 | 2:+25623310 | MsG0280008117.01.T01:CDS |
| GACCAATTGACACCAAAGGG+TGG | 0.701501 | 2:+25623121 | MsG0280008117.01.T01:CDS |
| GCGCCTCGGCAACAGCAGAG+TGG | 0.703474 | 2:+25623460 | MsG0280008117.01.T01:CDS |
| GGTCCTTTATGAACACCTAG+CGG | 0.739954 | 2:-25623286 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACCTTAAACTTTCAACA+AGG | + | Chr2:25623310-25623329 | MsG0280008117.01.T01:CDS | 25.0% |
| !! | GAATCAGAGAAATTTTCTAT+AGG | - | Chr2:25623025-25623044 | None:intergenic | 25.0% |
| !! | TGTTGAAAGTTTAAGGTAAT+AGG | - | Chr2:25623310-25623329 | None:intergenic | 25.0% |
| !! | TGTTTGTGTATGAATTTGTT+GGG | - | Chr2:25623096-25623115 | None:intergenic | 25.0% |
| ACTACCTTGTTGAAAGTTTA+AGG | - | Chr2:25623317-25623336 | None:intergenic | 30.0% | |
| AGATCTTCTTCTAATTGGTT+TGG | - | Chr2:25623155-25623174 | None:intergenic | 30.0% | |
| ATACTATCAACAAACTCTCA+TGG | + | Chr2:25622982-25623001 | MsG0280008117.01.T01:CDS | 30.0% | |
| ATCATAGTCTCTAATGGAAT+AGG | - | Chr2:25623067-25623086 | None:intergenic | 30.0% | |
| CTCAAAATCATAGTCTCTAA+TGG | - | Chr2:25623073-25623092 | None:intergenic | 30.0% | |
| GATAGTATTGCATCCAATAT+TGG | - | Chr2:25622970-25622989 | None:intergenic | 30.0% | |
| GTGTTTGTGTATGAATTTGT+TGG | - | Chr2:25623097-25623116 | None:intergenic | 30.0% | |
| ! | TCAGAGAAATTTTCTATAGG+TGG | - | Chr2:25623022-25623041 | None:intergenic | 30.0% |
| ! | TTGCACAAGTTGTCTAAAAT+TGG | - | Chr2:25623224-25623243 | None:intergenic | 30.0% |
| ! | TTGGTTGTGGATAATGATTT+TGG | + | Chr2:25623577-25623596 | MsG0280008117.01.T01:CDS | 30.0% |
| TCAGAAACATCCAAGTTACT+AGG | - | Chr2:25623553-25623572 | None:intergenic | 35.0% | |
| TTGGATATATTACCACCCTT+TGG | - | Chr2:25623136-25623155 | None:intergenic | 35.0% | |
| TTTGCATTGAGTAGTGTTGT+TGG | - | Chr2:25623196-25623215 | None:intergenic | 35.0% | |
| ! | AGTAACTTGGATGTTTCTGA+TGG | + | Chr2:25623553-25623572 | MsG0280008117.01.T01:CDS | 35.0% |
| ! | GCTCTAGATCTTCTTCTAAT+TGG | - | Chr2:25623160-25623179 | None:intergenic | 35.0% |
| AAGTGACTAAGCCATTTCCA+TGG | + | Chr2:25623419-25623438 | MsG0280008117.01.T01:CDS | 40.0% | |
| AGTGACCAATTGACACCAAA+GGG | + | Chr2:25623118-25623137 | MsG0280008117.01.T01:CDS | 40.0% | |
| ATTCGAGTAACTCGTTGTCA+AGG | + | Chr2:25623527-25623546 | MsG0280008117.01.T01:CDS | 40.0% | |
| CAACTTGTGCAACAATTCAC+AGG | + | Chr2:25623232-25623251 | MsG0280008117.01.T01:CDS | 40.0% | |
| CTGCTTCTACTGACCAATAT+TGG | + | Chr2:25622954-25622973 | MsG0280008117.01.T01:CDS | 40.0% | |
| GACAACGAGTTACTCGAATT+CGG | - | Chr2:25623526-25623545 | None:intergenic | 40.0% | |
| TAAGCCATTTCCATGGAACA+AGG | + | Chr2:25623426-25623445 | MsG0280008117.01.T01:CDS | 40.0% | |
| ! | CTTGGATGTTTCTGATGGTT+TGG | + | Chr2:25623558-25623577 | MsG0280008117.01.T01:CDS | 40.0% |
| ! | TGAATTTGCTCCTTGTTCCA+TGG | - | Chr2:25623439-25623458 | None:intergenic | 40.0% |
| !! | TGTTTCTGATGGTTTGGTTG+TGG | + | Chr2:25623564-25623583 | MsG0280008117.01.T01:CDS | 40.0% |
| CAGTGACCAATTGACACCAA+AGG | + | Chr2:25623117-25623136 | MsG0280008117.01.T01:CDS | 45.0% | |
| GACGAGGACAAAGTTGTACT+AGG | - | Chr2:25623262-25623281 | None:intergenic | 45.0% | |
| GGTCCTTTATGAACACCTAG+CGG | - | Chr2:25623289-25623308 | None:intergenic | 45.0% | |
| GTTGTCAAGGCCTAGTAACT+TGG | + | Chr2:25623540-25623559 | MsG0280008117.01.T01:CDS | 45.0% | |
| TGCTCCTTGTTCCATGGAAA+TGG | - | Chr2:25623433-25623452 | None:intergenic | 45.0% | |
| TTGGTCAGTAGAAGCAGTCA+TGG | - | Chr2:25622951-25622970 | None:intergenic | 45.0% | |
| !! | CTTTGGTGTCAATTGGTCAC+TGG | - | Chr2:25623119-25623138 | None:intergenic | 45.0% |
| !! | TACCACCCTTTGGTGTCAAT+TGG | - | Chr2:25623126-25623145 | None:intergenic | 45.0% |
| AGGAGCAAATTCAAGCGCCT+CGG | + | Chr2:25623446-25623465 | MsG0280008117.01.T01:CDS | 50.0% | |
| GACCAATTGACACCAAAGGG+TGG | + | Chr2:25623121-25623140 | MsG0280008117.01.T01:CDS | 50.0% | |
| TCGCCGCTAGGTGTTCATAA+AGG | + | Chr2:25623283-25623302 | MsG0280008117.01.T01:CDS | 50.0% | |
| TAACCACTCTGCTGTTGCCG+AGG | - | Chr2:25623466-25623485 | None:intergenic | 55.0% | |
| AACACCTAGCGGCGACGACG+AGG | - | Chr2:25623278-25623297 | None:intergenic | 65.0% | |
| TTGTCCTCGTCGTCGCCGCT+AGG | + | Chr2:25623271-25623290 | MsG0280008117.01.T01:CDS | 65.0% | |
| GCGCCTCGGCAACAGCAGAG+TGG | + | Chr2:25623460-25623479 | MsG0280008117.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 25622938 | 25623648 | 25622938 | ID=MsG0280008117.01;Name=MsG0280008117.01 |
| Chr2 | mRNA | 25622938 | 25623648 | 25622938 | ID=MsG0280008117.01.T01;Parent=MsG0280008117.01;Name=MsG0280008117.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|236 |
| Chr2 | exon | 25622938 | 25623648 | 25622938 | ID=MsG0280008117.01.T01:exon:14072;Parent=MsG0280008117.01.T01 |
| Chr2 | CDS | 25622938 | 25623648 | 25622938 | ID=MsG0280008117.01.T01:cds;Parent=MsG0280008117.01.T01 |
| Gene Sequence |
| Protein sequence |
