Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026066.01.T01 | KEH18875.1 | 72.84 | 81 | 22 | 0 | 14 | 94 | 64 | 144 | 1.18E-33 | 130 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026066.01.T01 | A0A072TZD4 | 72.840 | 81 | 22 | 0 | 14 | 94 | 64 | 144 | 5.64e-34 | 130 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005354.01 | MsG0580026066.01 | 0.815372 | 9.564241e-52 | 6.582105e-49 |
| MsG0580026066.01 | MsG0580026067.01 | 0.819977 | 8.749431e-53 | 6.835188e-50 |
| MsG0380017215.01 | MsG0580026066.01 | 0.808312 | 3.294211e-50 | 1.877384e-47 |
| MsG0380017229.01 | MsG0580026066.01 | 0.804412 | 2.187629e-49 | 1.127152e-46 |
| MsG0280007691.01 | MsG0580026066.01 | 0.804864 | 1.760491e-49 | 9.178154e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026066.01.T01 | MTR_8g031080 | 72.840 | 81 | 22 | 0 | 14 | 94 | 64 | 144 | 1.43e-37 | 130 |
| MsG0580026066.01.T01 | MTR_8g028785 | 50.000 | 94 | 24 | 2 | 1 | 94 | 1 | 71 | 6.47e-23 | 91.3 |
| MsG0580026066.01.T01 | MTR_3g097110 | 38.043 | 92 | 57 | 0 | 3 | 94 | 20 | 111 | 9.51e-14 | 63.9 |
| MsG0580026066.01.T01 | MTR_6g033560 | 37.647 | 85 | 53 | 0 | 10 | 94 | 179 | 263 | 1.56e-13 | 65.5 |
| MsG0580026066.01.T01 | MTR_2g079840 | 35.106 | 94 | 42 | 2 | 1 | 94 | 64 | 138 | 6.46e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCGTTAGGAGCGCATAT+CGG | 0.361392 | 5:-30118927 | MsG0580026066.01.T01:CDS |
| CTCCGTTAGGAGCGCATATC+GGG | 0.362386 | 5:-30118926 | MsG0580026066.01.T01:CDS |
| ACAAGTTAATAAGGATAGAT+TGG | 0.371938 | 5:-30118976 | MsG0580026066.01.T01:CDS |
| GCTGTTTCCACAAGTTAATA+AGG | 0.372095 | 5:-30118985 | MsG0580026066.01.T01:CDS |
| TGGTGAACCATGGTTGGGTA+CGG | 0.383423 | 5:-30119159 | MsG0580026066.01.T01:CDS |
| GGTGAACCATGGTTGGGTAC+GGG | 0.391972 | 5:-30119158 | MsG0580026066.01.T01:CDS |
| GACAGAGCATCCGGAGTTGC+AGG | 0.394787 | 5:+30119122 | None:intergenic |
| TTAATAAGGATAGATTGGTT+TGG | 0.413550 | 5:-30118971 | MsG0580026066.01.T01:CDS |
| TCGTATCTTGATGGATCAAA+TGG | 0.431896 | 5:+30119078 | None:intergenic |
| CTTATTGGTGAACCATGGTT+GGG | 0.434335 | 5:-30119164 | MsG0580026066.01.T01:CDS |
| TCTTATTGGTGAACCATGGT+TGG | 0.440218 | 5:-30119165 | MsG0580026066.01.T01:CDS |
| AGGAACAGCGCAGAAAATAT+TGG | 0.443459 | 5:-30119015 | MsG0580026066.01.T01:CDS |
| TCTGTCCATTCAAAGATATT+CGG | 0.456985 | 5:-30119105 | MsG0580026066.01.T01:CDS |
| CTTTGAATGGACAGAGCATC+CGG | 0.464790 | 5:+30119113 | None:intergenic |
| GCTTACCGAATATCTTTGAA+TGG | 0.466872 | 5:+30119100 | None:intergenic |
| AATCTATCCTTATTAACTTG+TGG | 0.472288 | 5:+30118978 | None:intergenic |
| AAAATGAACAATACTCCGTT+AGG | 0.472779 | 5:-30118939 | MsG0580026066.01.T01:CDS |
| AAGTATTCCTCCTGCAACTC+CGG | 0.483184 | 5:-30119132 | MsG0580026066.01.T01:CDS |
| TTTAGTGCGAAAATGGTAGT+TGG | 0.483825 | 5:-30119230 | None:intergenic |
| GTGAACCATGGTTGGGTACG+GGG | 0.512502 | 5:-30119157 | MsG0580026066.01.T01:CDS |
| AAAACGTGGAATGAAGAACT+TGG | 0.529480 | 5:-30119056 | MsG0580026066.01.T01:CDS |
| CATGGTGCGCGATGGAGGAT+TGG | 0.535001 | 5:-30119206 | MsG0580026066.01.T01:CDS |
| TTGGTCATGGTGCGCGATGG+AGG | 0.536307 | 5:-30119211 | MsG0580026066.01.T01:CDS |
| GGGAGATACATATTTGAGGC+AGG | 0.539227 | 5:-30119035 | MsG0580026066.01.T01:CDS |
| TACCCGATATGCGCTCCTAA+CGG | 0.563690 | 5:+30118924 | None:intergenic |
| GCGCGATGGAGGATTGGTAA+TGG | 0.575626 | 5:-30119200 | MsG0580026066.01.T01:CDS |
| ACTTGGGAGATACATATTTG+AGG | 0.579324 | 5:-30119039 | MsG0580026066.01.T01:CDS |
| AAACGTGGAATGAAGAACTT+GGG | 0.613512 | 5:-30119055 | MsG0580026066.01.T01:CDS |
| GCGAAAATGGTAGTTGGTCA+TGG | 0.619208 | 5:-30119224 | None:intergenic |
| TCTCTCTTATTGGTGAACCA+TGG | 0.619265 | 5:-30119169 | MsG0580026066.01.T01:CDS |
| CTTAACCCCGTACCCAACCA+TGG | 0.631884 | 5:+30119152 | None:intergenic |
| TAGTTGGTCATGGTGCGCGA+TGG | 0.645095 | 5:-30119214 | MsG0580026066.01.T01:CDS |
| AGAGCATCCGGAGTTGCAGG+AGG | 0.670364 | 5:+30119125 | None:intergenic |
| TCCATCAAGATACGAAAACG+TGG | 0.680647 | 5:-30119070 | MsG0580026066.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAGTTAATAAGGATAGAT+TGG | - | Chr5:30119159-30119178 | MsG0580026066.01.T01:CDS | 25.0% |
| !! | AATCTATCCTTATTAACTTG+TGG | + | Chr5:30119160-30119179 | None:intergenic | 25.0% |
| !! | TTAATAAGGATAGATTGGTT+TGG | - | Chr5:30119164-30119183 | MsG0580026066.01.T01:CDS | 25.0% |
| AAAATGAACAATACTCCGTT+AGG | - | Chr5:30119196-30119215 | MsG0580026066.01.T01:CDS | 30.0% | |
| TCTGTCCATTCAAAGATATT+CGG | - | Chr5:30119030-30119049 | MsG0580026066.01.T01:CDS | 30.0% | |
| !! | GGTTTTGATATCTCTCTTAT+TGG | - | Chr5:30118956-30118975 | MsG0580026066.01.T01:CDS | 30.0% |
| AAAACGTGGAATGAAGAACT+TGG | - | Chr5:30119079-30119098 | MsG0580026066.01.T01:CDS | 35.0% | |
| AAACGTGGAATGAAGAACTT+GGG | - | Chr5:30119080-30119099 | MsG0580026066.01.T01:CDS | 35.0% | |
| ACTTGGGAGATACATATTTG+AGG | - | Chr5:30119096-30119115 | MsG0580026066.01.T01:CDS | 35.0% | |
| GCTTACCGAATATCTTTGAA+TGG | + | Chr5:30119038-30119057 | None:intergenic | 35.0% | |
| ! | GCTGTTTCCACAAGTTAATA+AGG | - | Chr5:30119150-30119169 | MsG0580026066.01.T01:CDS | 35.0% |
| ! | TCGTATCTTGATGGATCAAA+TGG | + | Chr5:30119060-30119079 | None:intergenic | 35.0% |
| !!! | CGGAGTATTGTTCATTTTCT+CGG | + | Chr5:30119194-30119213 | None:intergenic | 35.0% |
| AGGAACAGCGCAGAAAATAT+TGG | - | Chr5:30119120-30119139 | MsG0580026066.01.T01:CDS | 40.0% | |
| CTTATTGGTGAACCATGGTT+GGG | - | Chr5:30118971-30118990 | MsG0580026066.01.T01:CDS | 40.0% | |
| TCCATCAAGATACGAAAACG+TGG | - | Chr5:30119065-30119084 | MsG0580026066.01.T01:CDS | 40.0% | |
| TCTCTCTTATTGGTGAACCA+TGG | - | Chr5:30118966-30118985 | MsG0580026066.01.T01:CDS | 40.0% | |
| TCTTATTGGTGAACCATGGT+TGG | - | Chr5:30118970-30118989 | MsG0580026066.01.T01:CDS | 40.0% | |
| ! | TCCACGTTTTCGTATCTTGA+TGG | + | Chr5:30119069-30119088 | None:intergenic | 40.0% |
| AAGTATTCCTCCTGCAACTC+CGG | - | Chr5:30119003-30119022 | MsG0580026066.01.T01:CDS | 45.0% | |
| ! | CTTTGAATGGACAGAGCATC+CGG | + | Chr5:30119025-30119044 | None:intergenic | 45.0% |
| ! | GGGAGATACATATTTGAGGC+AGG | - | Chr5:30119100-30119119 | MsG0580026066.01.T01:CDS | 45.0% |
| TACCCGATATGCGCTCCTAA+CGG | + | Chr5:30119214-30119233 | None:intergenic | 50.0% | |
| TGGTGAACCATGGTTGGGTA+CGG | - | Chr5:30118976-30118995 | MsG0580026066.01.T01:CDS | 50.0% | |
| ! | ACTCCGTTAGGAGCGCATAT+CGG | - | Chr5:30119208-30119227 | MsG0580026066.01.T01:CDS | 50.0% |
| CTTAACCCCGTACCCAACCA+TGG | + | Chr5:30118986-30119005 | None:intergenic | 55.0% | |
| GGTGAACCATGGTTGGGTAC+GGG | - | Chr5:30118977-30118996 | MsG0580026066.01.T01:CDS | 55.0% | |
| GTGAACCATGGTTGGGTACG+GGG | - | Chr5:30118978-30118997 | MsG0580026066.01.T01:CDS | 55.0% | |
| TAGTTGGTCATGGTGCGCGA+TGG | - | Chr5:30118921-30118940 | MsG0580026066.01.T01:CDS | 55.0% | |
| ! | CTCCGTTAGGAGCGCATATC+GGG | - | Chr5:30119209-30119228 | MsG0580026066.01.T01:CDS | 55.0% |
| ! | GCGCGATGGAGGATTGGTAA+TGG | - | Chr5:30118935-30118954 | MsG0580026066.01.T01:CDS | 55.0% |
| CATGGTGCGCGATGGAGGAT+TGG | - | Chr5:30118929-30118948 | MsG0580026066.01.T01:CDS | 60.0% | |
| TTGGTCATGGTGCGCGATGG+AGG | - | Chr5:30118924-30118943 | MsG0580026066.01.T01:CDS | 60.0% | |
| ! | AGAGCATCCGGAGTTGCAGG+AGG | + | Chr5:30119013-30119032 | None:intergenic | 60.0% |
| ! | GACAGAGCATCCGGAGTTGC+AGG | + | Chr5:30119016-30119035 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 30118917 | 30119240 | 30118917 | ID=MsG0580026066.01;Name=MsG0580026066.01 |
| Chr5 | mRNA | 30118917 | 30119240 | 30118917 | ID=MsG0580026066.01.T01;Parent=MsG0580026066.01;Name=MsG0580026066.01.T01;_AED=0.54;_eAED=0.69;_QI=0|-1|0|1|-1|1|1|0|107 |
| Chr5 | exon | 30118917 | 30119240 | 30118917 | ID=MsG0580026066.01.T01:exon:33748;Parent=MsG0580026066.01.T01 |
| Chr5 | CDS | 30118917 | 30119240 | 30118917 | ID=MsG0580026066.01.T01:cds;Parent=MsG0580026066.01.T01 |
| Gene Sequence |
| Protein sequence |
