AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027164.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027164.01.T01 MTR_0014s0220 65.455 385 103 4 1 384 232 587 4.29e-177 514
MsG0580027164.01.T01 MTR_2g016390 53.506 385 178 1 1 384 89 473 2.66e-141 418
MsG0580027164.01.T01 MTR_4g076760 52.308 390 180 2 1 384 110 499 1.65e-140 410
MsG0580027164.01.T01 MTR_5g032840 51.206 373 181 1 5 376 4 376 5.80e-135 392
MsG0580027164.01.T01 MTR_2g055890 67.286 269 87 1 1 269 139 406 4.27e-132 385
MsG0580027164.01.T01 MTR_7g050355 51.613 372 175 2 2 368 248 619 5.80e-131 389
MsG0580027164.01.T01 MTR_5g071700 52.809 356 159 2 1 355 9 356 3.69e-129 378
MsG0580027164.01.T01 MTR_1g057360 48.042 383 146 2 1 382 22 352 5.98e-119 349
MsG0580027164.01.T01 MTR_4g062370 50.267 374 166 3 1 372 316 671 9.47e-119 361
MsG0580027164.01.T01 MTR_0296s0010 51.125 311 150 2 76 384 1 311 5.87e-113 333
MsG0580027164.01.T01 MTR_4g037500 60.148 271 86 3 1 271 87 335 3.24e-110 326
MsG0580027164.01.T01 MTR_1g012040 47.899 357 184 2 1 356 66 421 4.96e-108 330
MsG0580027164.01.T01 MTR_2g064230 45.093 377 185 2 1 376 25 380 3.88e-104 317
MsG0580027164.01.T01 MTR_7g022020 44.032 377 165 3 1 376 223 554 7.41e-101 311
MsG0580027164.01.T01 MTR_5g061500 43.784 370 185 4 4 372 90 437 2.90e-98 301
MsG0580027164.01.T01 MTR_8g061330 42.779 367 172 4 4 369 77 406 2.87e-92 284
MsG0580027164.01.T01 MTR_1g021210 45.930 344 155 4 1 343 265 578 3.43e-92 288
MsG0580027164.01.T01 MTR_2g066670 45.455 319 173 1 41 358 50 368 4.90e-91 281
MsG0580027164.01.T01 MTR_3g034020 43.935 371 156 3 15 384 71 390 2.92e-90 279
MsG0580027164.01.T01 MTR_0003s0250 66.310 187 63 0 198 384 1 187 1.13e-88 267
MsG0580027164.01.T01 MTR_3g100990 48.289 263 136 0 122 384 182 444 3.00e-87 273
MsG0580027164.01.T01 MTR_7g027013 42.896 366 141 4 1 365 371 669 3.11e-87 278
MsG0580027164.01.T01 MTR_0002s0870 45.161 310 137 2 27 335 62 339 9.85e-86 264
MsG0580027164.01.T01 MTR_4g086180 38.442 385 197 7 1 384 67 412 1.34e-84 264
MsG0580027164.01.T01 MTR_1g092990 59.361 219 75 3 166 384 119 323 2.41e-82 256
MsG0580027164.01.T01 MTR_4g120080 42.560 336 162 4 1 335 424 729 1.23e-81 265
MsG0580027164.01.T01 MTR_5g032940 41.641 329 160 4 53 376 1 302 2.51e-81 253
MsG0580027164.01.T01 MTR_1g092930 56.621 219 81 3 166 384 118 322 1.72e-80 252
MsG0580027164.01.T01 MTR_0550s0010 46.207 290 125 2 95 384 4 262 4.07e-80 247
MsG0580027164.01.T01 MTR_1g093010 59.804 204 72 2 181 384 107 300 7.45e-80 249
MsG0580027164.01.T01 MTR_0003s0410 39.637 386 181 9 1 384 169 504 6.06e-79 258
MsG0580027164.01.T01 MTR_2g089470 39.944 358 171 6 1 351 429 749 1.06e-73 244
MsG0580027164.01.T01 MTR_2g068990 39.944 358 171 6 1 351 429 749 1.06e-73 244
MsG0580027164.01.T01 MTR_5g053570 73.418 158 41 1 1 158 311 467 2.76e-72 233
MsG0580027164.01.T01 MTR_5g059380 38.482 369 144 6 2 369 66 352 4.56e-72 230
MsG0580027164.01.T01 MTR_7g034270 41.967 305 153 4 1 302 67 350 1.15e-71 228
MsG0580027164.01.T01 MTR_2g058780 41.958 286 127 3 84 368 67 314 8.64e-71 224
MsG0580027164.01.T01 MTR_1g116150 38.310 355 138 5 16 369 47 321 9.51e-70 223
MsG0580027164.01.T01 MTR_0003s0390 44.238 269 138 2 1 268 115 372 2.16e-67 218
MsG0580027164.01.T01 MTR_4g096800 45.536 224 122 0 153 376 1 224 5.24e-67 214
MsG0580027164.01.T01 MTR_2g013330 44.094 254 119 1 131 384 197 427 1.01e-66 221
MsG0580027164.01.T01 MTR_0032s0020 37.134 307 143 2 79 384 310 567 1.03e-66 222
MsG0580027164.01.T01 MTR_8g022960 43.673 245 112 1 117 361 1 219 3.27e-65 209
MsG0580027164.01.T01 MTR_7g018160 39.796 294 113 3 91 383 3 233 7.56e-63 203
MsG0580027164.01.T01 MTR_1g054110 52.604 192 90 1 24 214 72 263 1.27e-62 202
MsG0580027164.01.T01 MTR_0017s0290 38.602 329 124 7 4 319 90 353 1.03e-60 201
MsG0580027164.01.T01 MTR_8g075630 50.000 176 88 0 209 384 2 177 2.43e-58 189
MsG0580027164.01.T01 MTR_6g029550 33.973 365 178 5 21 369 20 337 1.43e-57 193
MsG0580027164.01.T01 MTR_0073s0030 49.432 176 89 0 209 384 2 177 3.42e-57 186
MsG0580027164.01.T01 MTR_1g081340 42.259 239 135 2 127 365 112 347 3.54e-57 192
MsG0580027164.01.T01 MTR_6g055280 73.504 117 31 0 28 144 6 122 3.63e-57 184
MsG0580027164.01.T01 MTR_8g023250 38.246 285 147 3 89 372 1 257 8.15e-57 188
MsG0580027164.01.T01 MTR_8g469370 37.255 306 118 4 31 335 156 388 5.58e-56 189
MsG0580027164.01.T01 MTR_3g085530 46.667 195 104 0 182 376 547 741 3.84e-55 194
MsG0580027164.01.T01 MTR_3g085530 53.226 62 29 0 1 62 482 543 2.21e-11 65.9
MsG0580027164.01.T01 MTR_5g024850 36.389 360 134 7 11 369 75 340 2.62e-54 184
MsG0580027164.01.T01 MTR_3g031910 38.603 272 98 2 113 384 112 314 1.16e-53 181
MsG0580027164.01.T01 MTR_1g044190 40.316 253 111 3 1 252 226 439 2.90e-53 182
MsG0580027164.01.T01 MTR_5g087830 40.553 217 107 1 153 369 1 195 1.06e-52 176
MsG0580027164.01.T01 MTR_2g084465 37.500 288 122 5 72 358 72 302 1.77e-51 174
MsG0580027164.01.T01 MTR_3g052540 40.486 247 127 4 89 333 1 229 3.72e-51 173
MsG0580027164.01.T01 MTR_1g047970 44.845 194 107 0 191 384 2 195 1.59e-50 182
MsG0580027164.01.T01 MTR_8g045705 36.576 257 138 3 90 341 47 283 2.10e-50 171
MsG0580027164.01.T01 MTR_1g093700 34.853 307 147 4 72 372 2 261 2.89e-50 171
MsG0580027164.01.T01 MTR_0073s0010 52.326 172 81 1 1 171 75 246 1.64e-48 165
MsG0580027164.01.T01 MTR_3g113230 55.128 156 69 1 1 155 90 245 2.37e-48 164
MsG0580027164.01.T01 MTR_1g107470 48.677 189 80 2 1 188 41 213 4.30e-48 167
MsG0580027164.01.T01 MTR_1g107470 54.198 131 60 0 254 384 213 343 3.70e-41 149
MsG0580027164.01.T01 MTR_3g109050 45.455 176 80 1 209 384 2 161 9.64e-48 162
MsG0580027164.01.T01 MTR_2g049690 35.789 285 157 6 33 310 92 357 2.18e-47 165
MsG0580027164.01.T01 MTR_5g071480 36.934 287 110 6 84 369 34 250 2.25e-47 163
MsG0580027164.01.T01 MTR_7g052070 38.793 232 112 2 153 384 1 202 2.46e-46 158
MsG0580027164.01.T01 MTR_8g019090 44.444 207 75 1 153 359 1 167 4.05e-46 158
MsG0580027164.01.T01 MTR_3g048710 58.394 137 55 2 8 143 4 139 5.26e-46 155
MsG0580027164.01.T01 MTR_6g071545 36.800 250 106 2 1 249 37 235 5.77e-46 160
MsG0580027164.01.T01 MTR_6g071545 60.000 70 28 0 313 382 215 284 3.14e-23 99.4
MsG0580027164.01.T01 MTR_6g452450 45.238 168 92 0 191 358 2 169 7.24e-46 159
MsG0580027164.01.T01 MTR_3g109055 53.750 160 73 1 1 159 49 208 3.01e-45 155
MsG0580027164.01.T01 MTR_0134s0020 41.837 196 96 3 179 374 122 299 2.02e-44 157
MsG0580027164.01.T01 MTR_5g020370 55.172 145 48 4 1 144 87 215 4.25e-44 156
MsG0580027164.01.T01 MTR_5g020370 55.238 105 47 0 280 384 229 333 3.66e-36 135
MsG0580027164.01.T01 MTR_3g434910 39.574 235 116 5 1 232 125 336 8.13e-44 155
MsG0580027164.01.T01 MTR_0118s0040 51.449 138 67 0 231 368 151 288 2.26e-43 152
MsG0580027164.01.T01 MTR_2g064240 33.449 287 121 4 91 376 3 220 8.87e-43 150
MsG0580027164.01.T01 MTR_3g102890 61.290 124 47 1 1 123 41 164 2.76e-42 146
MsG0580027164.01.T01 MTR_0631s0010 59.524 126 50 1 1 125 128 253 8.60e-42 147
MsG0580027164.01.T01 MTR_7g113060 62.857 105 39 0 280 384 59 163 1.51e-41 145
MsG0580027164.01.T01 MTR_3g074460 59.292 113 43 1 275 384 47 159 8.81e-41 142
MsG0580027164.01.T01 MTR_2g058680 38.974 195 94 1 173 367 2 171 9.62e-41 143
MsG0580027164.01.T01 MTR_6g044370 38.462 195 102 2 162 351 75 256 2.63e-40 145
MsG0580027164.01.T01 MTR_7g070715 47.445 137 72 0 240 376 200 336 3.10e-40 147
MsG0580027164.01.T01 MTR_7g070715 55.319 94 42 0 1 94 89 182 4.97e-24 102
MsG0580027164.01.T01 MTR_3g030470 44.079 152 69 1 213 364 37 172 3.62e-40 142
MsG0580027164.01.T01 MTR_8g015280 49.600 125 63 0 259 383 19 143 9.90e-40 138
MsG0580027164.01.T01 MTR_4g078000 48.872 133 68 0 252 384 2 134 1.26e-39 141
MsG0580027164.01.T01 MTR_4g010800 55.238 105 47 0 280 384 28 132 9.99e-39 136
MsG0580027164.01.T01 MTR_8g071880 56.204 137 59 1 1 136 387 523 1.13e-38 145
MsG0580027164.01.T01 MTR_3g032470 35.827 254 112 5 92 344 4 207 2.22e-38 137
MsG0580027164.01.T01 MTR_4g009520 39.362 188 80 1 191 378 2 155 3.14e-38 136
MsG0580027164.01.T01 MTR_4g088095 58.261 115 47 1 4 117 69 183 6.47e-38 135
MsG0580027164.01.T01 MTR_1g092960 57.391 115 48 1 4 117 3 117 1.26e-37 132
MsG0580027164.01.T01 MTR_7g104240 46.154 143 62 1 227 369 149 276 1.54e-37 137
MsG0580027164.01.T01 MTR_7g060105 51.200 125 61 0 104 228 55 179 2.56e-37 133
MsG0580027164.01.T01 MTR_1g106700 45.638 149 81 0 210 358 23 171 2.59e-37 135
MsG0580027164.01.T01 MTR_4g115110 48.175 137 71 0 248 384 1 137 5.63e-37 132
MsG0580027164.01.T01 MTR_7g117680 51.282 117 57 0 252 368 4 120 7.05e-37 130
MsG0580027164.01.T01 MTR_4g037490 72.152 79 22 0 306 384 1 79 5.35e-36 127
MsG0580027164.01.T01 MTR_4g132830 46.763 139 59 1 231 369 184 307 1.11e-34 131
MsG0580027164.01.T01 MTR_4g132830 40.964 166 75 4 11 175 44 187 3.00e-22 97.1
MsG0580027164.01.T01 MTR_7g006890 45.000 140 68 2 229 368 96 226 5.92e-34 126
MsG0580027164.01.T01 MTR_4g123460 54.955 111 49 1 1 110 89 199 3.24e-33 123
MsG0580027164.01.T01 MTR_1g033220 41.830 153 75 2 178 330 103 241 3.41e-33 125
MsG0580027164.01.T01 MTR_1g033220 50.442 113 48 2 5 116 1 106 1.90e-26 107
MsG0580027164.01.T01 MTR_1g092940 52.381 105 33 1 280 384 3 90 2.05e-32 119
MsG0580027164.01.T01 MTR_8g469120 46.043 139 74 1 91 228 3 141 2.56e-32 119
MsG0580027164.01.T01 MTR_4g036720 34.167 240 103 3 1 240 75 259 4.51e-32 122
MsG0580027164.01.T01 MTR_0022s0370 54.639 97 44 0 288 384 65 161 5.13e-32 119
MsG0580027164.01.T01 MTR_7g108370 45.902 122 66 0 229 350 27 148 1.54e-31 117
MsG0580027164.01.T01 MTR_8g057880 51.515 99 47 1 272 369 47 145 5.30e-31 119
MsG0580027164.01.T01 MTR_0003s0260 68.675 83 26 0 1 83 144 226 6.53e-31 118
MsG0580027164.01.T01 MTR_6g024100 53.982 113 44 2 5 116 1 106 7.73e-31 118
MsG0580027164.01.T01 MTR_6g024100 36.310 168 80 1 178 345 103 243 1.02e-30 117
MsG0580027164.01.T01 MTR_4g034880 49.587 121 43 2 255 375 235 337 8.43e-31 122
MsG0580027164.01.T01 MTR_5g020340 55.340 103 45 1 283 384 260 362 1.86e-30 120
MsG0580027164.01.T01 MTR_3g103370 47.619 105 55 0 248 352 1 105 2.61e-29 110
MsG0580027164.01.T01 MTR_5g054110 37.791 172 84 5 55 226 3 151 4.57e-29 110
MsG0580027164.01.T01 MTR_3g462200 59.494 79 32 0 306 384 1 79 2.73e-28 106
MsG0580027164.01.T01 MTR_5g040660 48.246 114 46 1 27 140 85 185 5.06e-28 108
MsG0580027164.01.T01 MTR_8g085330 53.409 88 41 0 297 384 141 228 9.05e-28 110
MsG0580027164.01.T01 MTR_3g101850 36.628 172 70 3 205 376 44 176 2.46e-27 108
MsG0580027164.01.T01 MTR_3g045160 38.596 114 70 0 252 365 2 115 6.11e-27 103
MsG0580027164.01.T01 MTR_6g012610 58.228 79 33 0 306 384 1 79 2.94e-26 101
MsG0580027164.01.T01 MTR_1g033190 55.814 86 37 1 300 384 34 119 3.35e-26 102
MsG0580027164.01.T01 MTR_7g031700 32.669 251 108 6 1 249 129 320 3.43e-26 107
MsG0580027164.01.T01 MTR_7g039200 49.438 89 45 0 153 241 1 89 4.17e-26 100
MsG0580027164.01.T01 MTR_2g008505 42.593 108 60 1 243 350 197 302 2.76e-25 105
MsG0580027164.01.T01 MTR_2g008505 54.255 94 43 0 1 94 89 182 3.66e-24 102
MsG0580027164.01.T01 MTR_1g045920 38.255 149 69 2 202 350 98 223 2.81e-25 103
MsG0580027164.01.T01 MTR_5g077050 56.818 88 38 0 1 88 96 183 5.33e-25 101
MsG0580027164.01.T01 MTR_1g055385 54.639 97 41 2 153 249 1 94 7.89e-25 97.4
MsG0580027164.01.T01 MTR_7g110460 42.857 105 60 0 255 359 8 112 1.20e-24 100
MsG0580027164.01.T01 MTR_4g101340 56.338 71 31 0 306 376 1 71 3.74e-24 95.5
MsG0580027164.01.T01 MTR_7g062790 48.649 111 53 2 15 121 65 175 8.37e-24 99.8
MsG0580027164.01.T01 MTR_7g062790 31.169 154 86 5 127 271 112 254 7.41e-11 62.4
MsG0580027164.01.T01 MTR_6g008045 42.017 119 69 0 153 271 1 119 1.19e-23 96.3
MsG0580027164.01.T01 MTR_0025s0050 41.221 131 55 1 254 384 61 169 2.61e-23 96.7
MsG0580027164.01.T01 MTR_1g037600 48.810 84 42 1 267 349 150 233 4.03e-23 97.8
MsG0580027164.01.T01 MTR_0356s0050 31.088 193 84 4 32 224 63 206 1.83e-22 94.7
MsG0580027164.01.T01 MTR_5g009440 53.947 76 35 0 279 354 30 105 2.13e-22 93.2
MsG0580027164.01.T01 MTR_4g039550 40.870 115 64 2 247 359 11 123 5.27e-22 92.8
MsG0580027164.01.T01 MTR_6g033775 56.579 76 33 0 153 228 1 76 5.46e-22 89.4
MsG0580027164.01.T01 MTR_5g071420 47.573 103 52 2 282 384 101 201 7.78e-22 93.6
MsG0580027164.01.T01 MTR_7g490340 42.308 104 59 1 282 384 9 112 1.12e-21 90.1
MsG0580027164.01.T01 MTR_3g085980 35.417 144 54 2 227 368 28 134 2.76e-21 90.9
MsG0580027164.01.T01 MTR_3g114960 72.881 59 15 1 51 109 39 96 7.42e-21 87.4
MsG0580027164.01.T01 MTR_8g039580 52.632 76 36 0 279 354 30 105 1.41e-20 88.2
MsG0580027164.01.T01 MTR_3g098220 40.000 105 62 1 281 384 26 130 3.70e-20 86.3
MsG0580027164.01.T01 MTR_8g072100 48.571 70 36 0 280 349 56 125 5.93e-20 87.0
MsG0580027164.01.T01 MTR_3g095720 57.627 59 25 0 280 338 19 77 1.34e-19 84.7
MsG0580027164.01.T01 MTR_4g050743 32.258 186 68 4 117 301 16 144 9.39e-19 84.3
MsG0580027164.01.T01 MTR_2g058900 55.556 63 28 0 322 384 35 97 3.02e-18 80.5
MsG0580027164.01.T01 MTR_1g012410 57.377 61 26 0 278 338 5 65 4.13e-18 78.2
MsG0580027164.01.T01 MTR_2g047010 43.103 116 57 2 117 223 16 131 5.14e-18 82.0
MsG0580027164.01.T01 MTR_3g081620 57.895 57 24 0 328 384 1 57 1.29e-17 77.8
MsG0580027164.01.T01 MTR_2g026000 49.333 75 37 1 268 341 2 76 2.43e-17 77.0
MsG0580027164.01.T01 MTR_7g096440 29.545 176 119 3 193 366 67 239 3.06e-17 81.3
MsG0580027164.01.T01 MTR_6g061660 37.190 121 61 2 265 384 10 116 4.80e-17 77.0
MsG0580027164.01.T01 MTR_8g470080 50.000 82 38 1 1 82 37 115 4.88e-17 79.3
MsG0580027164.01.T01 MTR_8g470080 54.386 57 26 0 327 383 146 202 1.90e-14 72.0
MsG0580027164.01.T01 MTR_6g082730 53.125 64 30 0 27 90 85 148 2.21e-16 76.3
MsG0580027164.01.T01 MTR_4g134740 57.895 57 24 0 328 384 1 57 2.49e-16 75.1
MsG0580027164.01.T01 MTR_8g099480 51.316 76 27 1 153 228 1 66 4.13e-16 72.8
MsG0580027164.01.T01 MTR_7g070010 39.773 88 52 1 282 368 9 96 9.50e-16 72.8
MsG0580027164.01.T01 MTR_5g064790 48.438 64 33 0 306 369 1 64 1.53e-15 73.2
MsG0580027164.01.T01 MTR_3g112030 60.377 53 21 0 332 384 5 57 1.89e-15 71.6
MsG0580027164.01.T01 MTR_2g435290 40.909 88 44 2 60 147 57 136 3.56e-15 72.8
MsG0580027164.01.T01 MTR_3g046370 50.794 63 31 0 306 368 1 63 4.53e-15 71.2
MsG0580027164.01.T01 MTR_4g129060 48.000 75 38 1 74 147 2 76 6.08e-15 74.3
MsG0580027164.01.T01 MTR_6g082720 39.506 81 49 0 191 271 2 82 1.60e-14 68.9
MsG0580027164.01.T01 MTR_5g040670 39.506 81 49 0 191 271 2 82 1.60e-14 68.9
MsG0580027164.01.T01 MTR_1g098810 36.893 103 48 1 282 384 5 90 2.27e-14 70.1
MsG0580027164.01.T01 MTR_8g039750 51.667 60 29 0 306 365 1 60 2.29e-14 70.5
MsG0580027164.01.T01 MTR_1g106680 47.761 67 35 0 106 172 10 76 2.10e-13 65.5
MsG0580027164.01.T01 MTR_8g057780 42.857 63 36 0 305 367 3 65 5.29e-13 65.9
MsG0580027164.01.T01 MTR_7g117425 55.932 59 26 0 326 384 130 188 1.26e-12 67.8
MsG0580027164.01.T01 MTR_0057s0040 35.766 137 72 4 153 287 1 123 1.42e-12 64.7
MsG0580027164.01.T01 MTR_1g086460 44.444 54 30 0 310 363 51 104 2.04e-12 65.1
MsG0580027164.01.T01 MTR_1g051735 37.179 78 49 0 282 359 9 86 2.09e-12 63.2
MsG0580027164.01.T01 MTR_3g113220 38.462 78 48 0 210 287 3 80 6.54e-12 62.0
MsG0580027164.01.T01 MTR_2g083850 38.750 80 48 1 306 384 1 80 7.14e-12 62.0
MsG0580027164.01.T01 MTR_3g072880 41.250 80 26 2 306 384 1 60 5.78e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027164.01.T01 AT3G51700 43.056 216 120 2 170 384 60 273 6.16e-51 174
MsG0580027164.01.T01 AT3G51690 40.465 215 124 2 170 384 59 269 2.11e-48 167
MsG0580027164.01.T01 AT5G28780 41.627 209 117 3 178 384 66 271 5.55e-45 158

Find 54 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTATAAATGCTTCATATATT+TGG 0.087337 5:-55103305 MsG0580027164.01.T01:CDS
AAATTTCTTATCAGCATTAT+AGG 0.186625 5:+55102702 None:intergenic
AATTGACAAAATTAATAATA+TGG 0.213847 5:-55103515 MsG0580027164.01.T01:CDS
CATTCCATCAAACTTAAAAT+TGG 0.231346 5:-55102729 MsG0580027164.01.T01:CDS
CTACTTCCTCTTGGAATTAC+AGG 0.237065 5:+55103348 None:intergenic
GGAGGAAAGGTTGTATTCTT+TGG 0.283057 5:-55103393 MsG0580027164.01.T01:CDS
ATAGCAAAAGAAACAATAAT+TGG 0.309624 5:+55102501 None:intergenic
TCAGAATGGATATTGAAAAT+TGG 0.311483 5:-55103195 MsG0580027164.01.T01:CDS
CTAAAATAGCTCTACTTTGT+AGG 0.316920 5:+55103025 None:intergenic
TATAAATGCTTCATATATTT+GGG 0.326635 5:-55103304 MsG0580027164.01.T01:CDS
CATGGCCATTCAAGCTCATT+AGG 0.353396 5:-55102533 MsG0580027164.01.T01:CDS
GATGGTGACAATGACATTCT+TGG 0.358747 5:+55102564 None:intergenic
ATTGAAGCTAAAATAATTAC+AGG 0.363314 5:-55102615 MsG0580027164.01.T01:CDS
AAGTCTTGTAACAATTAATC+TGG 0.378266 5:+55102650 None:intergenic
TTTGAAATTAAAAGTTCATC+AGG 0.382522 5:+55103123 None:intergenic
TTGAGTTCTCTAAAATCATC+TGG 0.383659 5:-55102762 MsG0580027164.01.T01:CDS
CAAATTGAATTTCCAATGGT+TGG 0.387298 5:+55103576 None:intergenic
AAGAACATGAGGTTACAAAA+TGG 0.389542 5:-55103255 MsG0580027164.01.T01:CDS
ATTTCTGCAAACCCATCGTT+TGG 0.405716 5:+55103153 None:intergenic
GAATTTCCAATGGTTGGAAC+TGG 0.409060 5:+55103582 None:intergenic
AAGATTCCAGTTCCAACCAT+TGG 0.427077 5:-55103588 MsG0580027164.01.T01:CDS
GTGAAATGATTCAATAAATC+CGG 0.430404 5:+55103060 None:intergenic
TATAGAGAGGGGCTCTGAAC+TGG 0.437456 5:-55103550 MsG0580027164.01.T01:CDS
AAACAGAATTTGTGAGCCAT+TGG 0.449690 5:+55103483 None:intergenic
ATTGACAAAATTAATAATAT+GGG 0.461331 5:-55103514 MsG0580027164.01.T01:CDS
TATTGTTAAAAGTACATATC+CGG 0.464895 5:-55103079 MsG0580027164.01.T01:CDS
GAATGTGCTGTCCTTCCTCC+AGG 0.470881 5:+55103618 None:intergenic
TTAAGTTTGATGGAATGGTT+TGG 0.472098 5:+55102735 None:intergenic
ATGTCTGATCTACTTCCTCT+TGG 0.490647 5:+55103339 None:intergenic
TGCTATACCGCTTGATGCCA+CGG 0.497217 5:+55103653 None:intergenic
TGTCTCCTAATGAGCTTGAA+TGG 0.498040 5:+55102528 None:intergenic
TACATACTGAATCACATTCC+AGG 0.500277 5:-55102967 MsG0580027164.01.T01:intron
AATGAGTTATGACAAATCTT+CGG 0.518835 5:-55103430 MsG0580027164.01.T01:CDS
TTCAATTTGTGATATAGAGA+GGG 0.519774 5:-55103562 MsG0580027164.01.T01:CDS
CAAGCTGAGGGGTTATGTAA+TGG 0.530928 5:-55102675 MsG0580027164.01.T01:CDS
TGGATATTGAAAATTGGAGA+TGG 0.532966 5:-55103189 MsG0580027164.01.T01:CDS
ATCACAAATTGAATTTCCAA+TGG 0.537390 5:+55103572 None:intergenic
GGAAAGGTTGTATTCTTTGG+TGG 0.541209 5:-55103390 MsG0580027164.01.T01:CDS
TTTAGAGAACTCAAGAACTC+TGG 0.545103 5:+55102771 None:intergenic
ATTCAATTTGTGATATAGAG+AGG 0.567633 5:-55103563 MsG0580027164.01.T01:CDS
AAATTTCAGAACCAAACGAT+GGG 0.572052 5:-55103164 MsG0580027164.01.T01:CDS
TAACTCTAACAAAGAACATG+AGG 0.577765 5:-55103266 MsG0580027164.01.T01:CDS
AGAAATTTAGATCAAGCTGA+GGG 0.578046 5:-55102687 MsG0580027164.01.T01:CDS
AATATGGGATGAAGCTCCAA+TGG 0.580485 5:-55103499 MsG0580027164.01.T01:CDS
CTAATGAGCTTGAATGGCCA+TGG 0.584714 5:+55102534 None:intergenic
AAAATTTCAGAACCAAACGA+TGG 0.587733 5:-55103165 MsG0580027164.01.T01:CDS
AAGAAATTTAGATCAAGCTG+AGG 0.608134 5:-55102688 MsG0580027164.01.T01:CDS
AATGGCCATGGTGATTCTGA+TGG 0.615887 5:+55102546 None:intergenic
TACTTCCTCTTGGAATTACA+GGG 0.617065 5:+55103349 None:intergenic
TCAATTTGTGATATAGAGAG+GGG 0.639846 5:-55103561 MsG0580027164.01.T01:CDS
ATCCTCCCTGTAATTCCAAG+AGG 0.654577 5:-55103354 MsG0580027164.01.T01:CDS
TTCCTCTTGGAATTACAGGG+AGG 0.697482 5:+55103352 None:intergenic
GAAATTTAGATCAAGCTGAG+GGG 0.714066 5:-55102686 MsG0580027164.01.T01:CDS
TGTCACCATCAGAATCACCA+TGG 0.725989 5:-55102551 MsG0580027164.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGACAAAATTAATAATA+TGG - Chr5:55102669-55102688 MsG0580027164.01.T01:CDS 10.0%
!! ATTGACAAAATTAATAATAT+GGG - Chr5:55102670-55102689 MsG0580027164.01.T01:CDS 10.0%
!! ATTATATGAATATCACAATA+CGG + Chr5:55103285-55103304 None:intergenic 15.0%
!! TATAAATGCTTCATATATTT+GGG - Chr5:55102880-55102899 MsG0580027164.01.T01:intron 15.0%
!!! TGTACAATTTTTTTTTAAAC+AGG - Chr5:55103311-55103330 MsG0580027164.01.T01:CDS 15.0%
!!! TTATTAATTTTGTCAATTGT+AGG + Chr5:55102667-55102686 None:intergenic 15.0%
!! AAATTTCTTATCAGCATTAT+AGG + Chr5:55103485-55103504 None:intergenic 20.0%
!! ATAATTACAGGAAAAAACAT+TGG - Chr5:55103581-55103600 MsG0580027164.01.T01:CDS 20.0%
!! ATAGCAAAAGAAACAATAAT+TGG + Chr5:55103686-55103705 None:intergenic 20.0%
!! ATTGAAGCTAAAATAATTAC+AGG - Chr5:55103569-55103588 MsG0580027164.01.T01:CDS 20.0%
!! CTATAAATGCTTCATATATT+TGG - Chr5:55102879-55102898 MsG0580027164.01.T01:intron 20.0%
!!! TATTGTTAAAAGTACATATC+CGG - Chr5:55103105-55103124 MsG0580027164.01.T01:CDS 20.0%
!!! TTTGAAATTAAAAGTTCATC+AGG + Chr5:55103064-55103083 None:intergenic 20.0%
! AAAGAAGAATTAGAAATACC+TGG + Chr5:55103238-55103257 None:intergenic 25.0%
! AATGAGTTATGACAAATCTT+CGG - Chr5:55102754-55102773 MsG0580027164.01.T01:CDS 25.0%
! ATCAAAAATATTTGGAGGAA+AGG - Chr5:55102778-55102797 MsG0580027164.01.T01:CDS 25.0%
! ATCACAAATTGAATTTCCAA+TGG + Chr5:55102615-55102634 None:intergenic 25.0%
! ATTCAATTTGTGATATAGAG+AGG - Chr5:55102621-55102640 MsG0580027164.01.T01:CDS 25.0%
! CATTCCATCAAACTTAAAAT+TGG - Chr5:55103455-55103474 MsG0580027164.01.T01:CDS 25.0%
! GTGAAATGATTCAATAAATC+CGG + Chr5:55103127-55103146 None:intergenic 25.0%
! TCAGAATGGATATTGAAAAT+TGG - Chr5:55102989-55103008 MsG0580027164.01.T01:CDS 25.0%
! TTCAATTTGTGATATAGAGA+GGG - Chr5:55102622-55102641 MsG0580027164.01.T01:CDS 25.0%
!! AAGTCTTGTAACAATTAATC+TGG + Chr5:55103537-55103556 None:intergenic 25.0%
!! AGATTAGATTGTTTTCAGAA+TGG - Chr5:55102975-55102994 MsG0580027164.01.T01:CDS 25.0%
!! ATAGCATTTTTACTATTACC+TGG - Chr5:55102548-55102567 MsG0580027164.01.T01:CDS 25.0%
!!! AATTTTTTTTTAAACAGGCG+AGG - Chr5:55103316-55103335 MsG0580027164.01.T01:CDS 25.0%
!!! CAATTTTAAGTTTGATGGAA+TGG + Chr5:55103457-55103476 None:intergenic 25.0%
!!! TATTTTAGCATCAACAATTG+AGG - Chr5:55103174-55103193 MsG0580027164.01.T01:CDS 25.0%
!!! TTTTAACAATAGCATCGATT+GGG + Chr5:55103096-55103115 None:intergenic 25.0%
AAAATTTCAGAACCAAACGA+TGG - Chr5:55103019-55103038 MsG0580027164.01.T01:CDS 30.0%
AAATTTCAGAACCAAACGAT+GGG - Chr5:55103020-55103039 MsG0580027164.01.T01:CDS 30.0%
AAGAAATTTAGATCAAGCTG+AGG - Chr5:55103496-55103515 MsG0580027164.01.T01:CDS 30.0%
AAGAACATGAGGTTACAAAA+TGG - Chr5:55102929-55102948 MsG0580027164.01.T01:intron 30.0%
AGAAATTTAGATCAAGCTGA+GGG - Chr5:55103497-55103516 MsG0580027164.01.T01:CDS 30.0%
CAAATTGAATTTCCAATGGT+TGG + Chr5:55102611-55102630 None:intergenic 30.0%
TAACTCTAACAAAGAACATG+AGG - Chr5:55102918-55102937 MsG0580027164.01.T01:intron 30.0%
TCAATTTGTGATATAGAGAG+GGG - Chr5:55102623-55102642 MsG0580027164.01.T01:CDS 30.0%
TCTTCGGCATCAAAAATATT+TGG - Chr5:55102770-55102789 MsG0580027164.01.T01:CDS 30.0%
TGGATATTGAAAATTGGAGA+TGG - Chr5:55102995-55103014 MsG0580027164.01.T01:CDS 30.0%
TTAAGTTTGATGGAATGGTT+TGG + Chr5:55103452-55103471 None:intergenic 30.0%
! CTAAAATAGCTCTACTTTGT+AGG + Chr5:55103162-55103181 None:intergenic 30.0%
!! TTGAGTTCTCTAAAATCATC+TGG - Chr5:55103422-55103441 MsG0580027164.01.T01:CDS 30.0%
!!! AGTGCCAATTTTAAGTTTGA+TGG + Chr5:55103462-55103481 None:intergenic 30.0%
!!! CAAATTCTGTTTTGAAGCTT+TGG - Chr5:55102712-55102731 MsG0580027164.01.T01:CDS 30.0%
!!! CTTTTAACAATAGCATCGAT+TGG + Chr5:55103097-55103116 None:intergenic 30.0%
AAACAGAATTTGTGAGCCAT+TGG + Chr5:55102704-55102723 None:intergenic 35.0%
GAAATTTAGATCAAGCTGAG+GGG - Chr5:55103498-55103517 MsG0580027164.01.T01:CDS 35.0%
TACATACTGAATCACATTCC+AGG - Chr5:55103217-55103236 MsG0580027164.01.T01:CDS 35.0%
TACTTCCTCTTGGAATTACA+GGG + Chr5:55102838-55102857 None:intergenic 35.0%
TCGGCATCAAAAATATTTGG+AGG - Chr5:55102773-55102792 MsG0580027164.01.T01:CDS 35.0%
TTTAGAGAACTCAAGAACTC+TGG + Chr5:55103416-55103435 None:intergenic 35.0%
! GCATTTTTACTATTACCTGG+AGG - Chr5:55102551-55102570 MsG0580027164.01.T01:CDS 35.0%
! TTTTACTATTACCTGGAGGA+AGG - Chr5:55102555-55102574 MsG0580027164.01.T01:CDS 35.0%
AAGATTCCAGTTCCAACCAT+TGG - Chr5:55102596-55102615 MsG0580027164.01.T01:CDS 40.0%
AATATGGGATGAAGCTCCAA+TGG - Chr5:55102685-55102704 MsG0580027164.01.T01:CDS 40.0%
ATGTCTGATCTACTTCCTCT+TGG + Chr5:55102848-55102867 None:intergenic 40.0%
ATTTCTGCAAACCCATCGTT+TGG + Chr5:55103034-55103053 None:intergenic 40.0%
CTACTTCCTCTTGGAATTAC+AGG + Chr5:55102839-55102858 None:intergenic 40.0%
GAATTTCCAATGGTTGGAAC+TGG + Chr5:55102605-55102624 None:intergenic 40.0%
GATGGTGACAATGACATTCT+TGG + Chr5:55103623-55103642 None:intergenic 40.0%
GGAAAGGTTGTATTCTTTGG+TGG - Chr5:55102794-55102813 MsG0580027164.01.T01:CDS 40.0%
GGAGGAAAGGTTGTATTCTT+TGG - Chr5:55102791-55102810 MsG0580027164.01.T01:CDS 40.0%
TGTCTCCTAATGAGCTTGAA+TGG + Chr5:55103659-55103678 None:intergenic 40.0%
! TTTTTAAACAGGCGAGGCAA+AGG - Chr5:55103322-55103341 MsG0580027164.01.T01:CDS 40.0%
!! ATCGCGTTTTGAACGTAAAG+CGG + Chr5:55102501-55102520 None:intergenic 40.0%
AATGGCCATGGTGATTCTGA+TGG + Chr5:55103641-55103660 None:intergenic 45.0%
ATCCTCCCTGTAATTCCAAG+AGG - Chr5:55102830-55102849 MsG0580027164.01.T01:CDS 45.0%
CAAGCTGAGGGGTTATGTAA+TGG - Chr5:55103509-55103528 MsG0580027164.01.T01:CDS 45.0%
CATGGCCATTCAAGCTCATT+AGG - Chr5:55103651-55103670 MsG0580027164.01.T01:CDS 45.0%
CTAATGAGCTTGAATGGCCA+TGG + Chr5:55103653-55103672 None:intergenic 45.0%
TGTCACCATCAGAATCACCA+TGG - Chr5:55103633-55103652 MsG0580027164.01.T01:CDS 45.0%
TTCCTCTTGGAATTACAGGG+AGG + Chr5:55102835-55102854 None:intergenic 45.0%
!!! ACGCGATATTGTTTTGACCG+TGG - Chr5:55102514-55102533 MsG0580027164.01.T01:CDS 45.0%
TGCTATACCGCTTGATGCCA+CGG + Chr5:55102534-55102553 None:intergenic 50.0%
!! GTTTTGACCGTGGCATCAAG+CGG - Chr5:55102524-55102543 MsG0580027164.01.T01:CDS 50.0%
!! TATAGAGAGGGGCTCTGAAC+TGG - Chr5:55102634-55102653 MsG0580027164.01.T01:CDS 50.0%
GAATGTGCTGTCCTTCCTCC+AGG + Chr5:55102569-55102588 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 55102479 55103727 55102479 ID=MsG0580027164.01;Name=MsG0580027164.01
Chr5 mRNA 55102479 55103727 55102479 ID=MsG0580027164.01.T01;Parent=MsG0580027164.01;Name=MsG0580027164.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|384
Chr5 exon 55102968 55103727 55102968 ID=MsG0580027164.01.T01:exon:12404;Parent=MsG0580027164.01.T01
Chr5 exon 55102479 55102873 55102479 ID=MsG0580027164.01.T01:exon:12403;Parent=MsG0580027164.01.T01
Chr5 CDS 55102968 55103727 55102968 ID=MsG0580027164.01.T01:cds;Parent=MsG0580027164.01.T01
Chr5 CDS 55102479 55102873 55102479 ID=MsG0580027164.01.T01:cds;Parent=MsG0580027164.01.T01
Gene Sequence

>MsG0580027164.01.T01

ATGTGGAATACTCTTGCATCCGCTTTACGTTCAAAACGCGATATTGTTTTGACCGTGGCATCAAGCGGTATAGCATTTTTACTATTACCTGGAGGAAGGACAGCACATTCAAAGTTCAAGATTCCAGTTCCAACCATTGGAAATTCAATTTGTGATATAGAGAGGGGCTCTGAACTGGCACAACTCCTACAATTGACAAAATTAATAATATGGGATGAAGCTCCAATGGCTCACAAATTCTGTTTTGAAGCTTTGGACAGATCTTTAAGAGACATAATGAGTTATGACAAATCTTCGGCATCAAAAATATTTGGAGGAAAGGTTGTATTCTTTGGTGGTGATTTTAGACAGATCCTCCCTGTAATTCCAAGAGGAAGTAGATCAGACATTGTTCATGCAACTATAAATGCTTCATATATTTGGGATCATTGTACTGTTTTAACTCTAACAAAGAACATGAGGTTACAAAATGGATCATCAAATTCAAACTCTGAAGAGATTAGATTGTTTTCAGAATGGATATTGAAAATTGGAGATGGAAAAATTTCAGAACCAAACGATGGGTTTGCAGAAATCAAAATTCCTGATGAACTTTTAATTTCAAATTTTAATGACCCAATCGATGCTATTGTTAAAAGTACATATCCGGATTTATTGAATCATTTCACTGATGCTCAATTCCTACAAAGTAGAGCTATTTTAGCATCAACAATTGAGGTCGTTGATGAAATCAATGATTACATACTGAATCACATTCCAGGCGAGGCAAAGGATTATCACAGTTCAAACTCAATAGATCGAACAGAAGCTAATGATAATGAAGCATTTGAACAATTGACACCAGAGTTCTTGAGTTCTCTAAAATCATCTGGACTGCCAAACCATTCCATCAAACTTAAAATTGGCACTCCTATAATGCTGATAAGAAATTTAGATCAAGCTGAGGGGTTATGTAATGGTACCAGATTAATTGTTACAAGACTTGCAAATCATGTAATTGAAGCTAAAATAATTACAGGAAAAAACATTGGAAATTTGATCTACATTCCAAGAATGTCATTGTCACCATCAGAATCACCATGGCCATTCAAGCTCATTAGGAGACAATTTCCAATTATTGTTTCTTTTGCTATGACAATAAACAAATCACAATGA
Protein sequence

>MsG0580027164.01.T01

MWNTLASALRSKRDIVLTVASSGIAFLLLPGGRTAHSKFKIPVPTIGNSICDIERGSELAQLLQLTKLIIWDEAPMAHKFCFEALDRSLRDIMSYDKSSASKIFGGKVVFFGGDFRQILPVIPRGSRSDIVHATINASYIWDHCTVLTLTKNMRLQNGSSNSNSEEIRLFSEWILKIGDGKISEPNDGFAEIKIPDELLISNFNDPIDAIVKSTYPDLLNHFTDAQFLQSRAILASTIEVVDEINDYILNHIPGEAKDYHSSNSIDRTEANDNEAFEQLTPEFLSSLKSSGLPNHSIKLKIGTPIMLIRNLDQAEGLCNGTRLIVTRLANHVIEAKIITGKNIGNLIYIPRMSLSPSESPWPFKLIRRQFPIIVSFAMTINKSQ*