AlfalfaGEDB | MsG0580027410.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027410.01

KEGG: K15397
GO: GO:0003824 GO:0006633 GO:0008152 GO:0016020 GO:0016747
PFAM: PF08392 PF08541

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027410.01.T01	XP_003614634.1	99.518	415	2	0	1	415	82	496	0	861

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027410.01.T01	Q9XF43	85.06	415	62	0	1	415	83	497	0	761

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027410.01.T01	G7K1J8	99.518	415	2	0	1	415	82	496	0.0	861

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047955.01	MsG0580027410.01	0.824433	8.091364e-54	7.165658e-51
MsG0080047956.01	MsG0580027410.01	0.821377	4.170490e-53	3.387105e-50
MsG0080048059.01	MsG0580027410.01	0.821105	4.818686e-53	3.884301e-50
MsG0080048068.01	MsG0580027410.01	0.807375	5.213282e-50	2.898894e-47
MsG0080048198.01	MsG0580027410.01	0.810745	9.892373e-51	6.012301e-48
MsG0080048528.01	MsG0580027410.01	0.852392	4.756480e-61	9.924364e-58
MsG0080048611.01	MsG0580027410.01	0.801850	7.411634e-49	3.576712e-46
MsG0080048776.01	MsG0580027410.01	0.821276	4.400961e-53	3.564143e-50
MsG0080048777.01	MsG0580027410.01	0.820839	5.547595e-53	4.438861e-50
MsG0080048780.01	MsG0580027410.01	0.823780	1.152118e-53	1.001758e-50

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PPI

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Gene1	Gene2	Type
MsG0280006369.01	MsG0580027410.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027410.01.T01	MTR_5g056510	99.518	415	2	0	1	415	82	496	0.0	861
MsG0580027410.01.T01	MTR_1g110930	92.771	415	30	0	1	415	82	496	0.0	814
MsG0580027410.01.T01	MTR_4g070270	68.704	409	128	0	1	409	94	502	0.0	611
MsG0580027410.01.T01	MTR_1g041085	65.385	416	143	1	1	415	103	518	0.0	572
MsG0580027410.01.T01	MTR_1g076430	63.942	416	149	1	1	415	97	512	0.0	570
MsG0580027410.01.T01	MTR_2g054940	65.133	413	143	1	1	412	96	508	0.0	567
MsG0580027410.01.T01	MTR_7g118170	64.146	410	139	3	7	410	122	529	0.0	555
MsG0580027410.01.T01	MTR_4g113280	61.836	414	144	3	1	412	85	486	0.0	539
MsG0580027410.01.T01	MTR_7g081695	63.391	407	136	2	1	406	96	490	0.0	531
MsG0580027410.01.T01	MTR_3g105550	56.905	420	170	3	1	409	117	536	2.87e-180	513

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0580027410.01.T01	AT1G68530	85.060	415	62	0	1	415	83	497	761
MsG0580027410.01.T01	AT1G25450	82.892	415	71	0	1	415	78	492	752
MsG0580027410.01.T01	AT1G19440	68.059	407	130	0	1	407	106	512	615
MsG0580027410.01.T01	AT2G16280	68.460	409	129	0	1	409	102	510	613
MsG0580027410.01.T01	AT4G34510	65.122	410	142	1	1	409	76	485	578
MsG0580027410.01.T01	AT2G26640	62.228	413	155	1	1	412	94	506	551
MsG0580027410.01.T01	AT1G04220	62.500	416	148	3	3	410	101	516	548
MsG0580027410.01.T01	AT5G43760	63.007	419	146	4	3	413	107	524	543
MsG0580027410.01.T01	AT1G01120	60.827	411	159	1	3	411	118	528	542
MsG0580027410.01.T01	AT2G15090	57.554	417	169	2	1	409	63	479	520

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Find 80 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGACGCGGCAAGTAAT+CGG	0.440585	5:+60022429	None:intergenic
AAATCCAATATAACAACTAT+TGG	0.388103	5:-60021703	MsG0580027410.01.T01:CDS
AACTCCTTCTTTGTCTGCTA+TGG	0.395630	5:-60022136	MsG0580027410.01.T01:CDS
AACTGTTTATTCAGAATGGG+TGG	0.613269	5:-60021916	MsG0580027410.01.T01:CDS
AAGAAGGAGTTGGAGAAAAG+AGG	0.546500	5:+60022148	None:intergenic
AAGAGTAGTTGTTCTGAAGC+AGG	0.493059	5:+60021667	None:intergenic
AAGATCGCGGGCTAAATCGA+TGG	0.572509	5:+60022035	None:intergenic
AAGGATCTTATGGCTATAGC+AGG	0.535171	5:-60021736	MsG0580027410.01.T01:CDS
ACAACAACTGCGTTTGAATT+CGG	0.360073	5:+60022000	None:intergenic
ACAATCTCAGGAATATGAAC+AGG	0.469213	5:+60021265	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACCAAACTGTTTATTCAGAA+TGG	-	Chr5:60021815-60021834	MsG0580027410.01.T01:CDS	30.0%
AGGACCAATAGTTGTTATAT+TGG	+	Chr5:60022039-60022058	None:intergenic	30.0%
AGTTGGAATTTCACTTCAAA+AGG	-	Chr5:60021980-60021999	MsG0580027410.01.T01:CDS	30.0%
ATAAGAATGTCTATGTCCTT+AGG	+	Chr5:60021555-60021574	None:intergenic	30.0%
ATTACTCCAAACTATTACCA+AGG	-	Chr5:60021771-60021790	MsG0580027410.01.T01:CDS	30.0%
CCAAACTGTTTATTCAGAAT+GGG	-	Chr5:60021816-60021835	MsG0580027410.01.T01:CDS	30.0%
TTCACTTCAAAAGGATCTTA+TGG	-	Chr5:60021989-60022008	MsG0580027410.01.T01:CDS	30.0%
AACTGTTTATTCAGAATGGG+TGG	-	Chr5:60021819-60021838	MsG0580027410.01.T01:CDS	35.0%
ACAACAACTGCGTTTGAATT+CGG	+	Chr5:60021738-60021757	None:intergenic	35.0%
ACAATCTCAGGAATATGAAC+AGG	+	Chr5:60022473-60022492	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	60021255	60022502	60021255	ID=MsG0580027410.01;Name=MsG0580027410.01
Chr5	mRNA	60021255	60022502	60021255	ID=MsG0580027410.01.T01;Parent=MsG0580027410.01;Name=MsG0580027410.01.T01;_AED=0.31;_eAED=0.31;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|415
Chr5	exon	60021255	60022502	60021255	ID=MsG0580027410.01.T01:exon:22247;Parent=MsG0580027410.01.T01
Chr5	CDS	60021255	60022502	60021255	ID=MsG0580027410.01.T01:cds;Parent=MsG0580027410.01.T01

Gene Sequence

>MsG0580027410.01.T01

ATGTTAAAGCCACGCGCAATCTACCTAGTCGATTATGCCTGCTTCAAACCACCGATTACTTGCCGCGTCCCTTTCGCTACTTTCATGGAACATTCAAGACTAATCCTCAAGAACAACCCGAAAAGCGTTGAGTTCCAAATGAGAATTCTCGAACGCTCTGGTCTTGGAGAAGAAACATGTTTGCCACCAGCAATCCATTACATCCCACCTAAACCAACAATGGAAGAAGCAAGACGCGAAGCCGAGCTTGTTATCTTCTCATCCATGGATACCTTGTTTGCGAAAACCGGACTCAAACCTAAGGACATAGACATTCTTATAGTCAATTGCAGCCTCTTTTCTCCAACTCCTTCTTTGTCTGCTATGGTGATCAACAAATACAAACTTAGAAGCAACATCAAGAGCTTCAACCTCTCCGGAATGGGATGTAGCGCGGGTTTAATCTCCATCGATTTAGCCCGCGATCTTCTTCAGGTTTATCCGAATTCAAACGCAGTTGTTGTGAGCACAGAAATCATTACTCCAAACTATTACCAAGGAAAAGAAAGAGCAATGCTTTTACCAAACTGTTTATTCAGAATGGGTGGTGCTGCTATTCTTTTATCAAACAAAAAAACAGAACACAAAAGAGCAAAATATAGATTGATGCAAGTTGTTAGAACTCACAAAGGAGGTGATGATAAAGCTTACCGTTGCGTTTTCGAAGAAGAAGACAGAGAAGGTAAAGTTGGAATTTCACTTCAAAAGGATCTTATGGCTATAGCAGGAGAAGCATTGAAATCCAATATAACAACTATTGGTCCTCTTGTTCTACCTGCTTCAGAACAACTACTCTTTCTTTTCACACTCATTGGAAAGAAAATCTTCAATCCTAAAATGAAGCCTTATATTCCTGATTTCAAACAAGCGTTTGAGCATTTTTGTATTCATGCTGGTGGACGTGCCGTGATCGATGAGTTACAGAAGAATCTTCAGCTATCTGCTGAACATGTCGAGGCTTCAAGAATGACGCTGCATCGATTCGGGAACACGTCTTCTTCATCTTTATGGTATGAATTGAACTATATTGAATCAAAAGGAAGGATGAAGAGAGGTGATAGAGTATGGCAAATAGCTTTTGGAAGTGGATTTAAATGCAATAGTGCTGTGTGGAAATGTAATAGAAGCATTAAGACACCTGTTAATGGACCTTGGGAAGATTGCATTGATCGCTATCCTGTTCATATTCCTGAGATTGTTAAGCTCTAA

Protein sequence

>MsG0580027410.01.T01

MLKPRAIYLVDYACFKPPITCRVPFATFMEHSRLILKNNPKSVEFQMRILERSGLGEETCLPPAIHYIPPKPTMEEARREAELVIFSSMDTLFAKTGLKPKDIDILIVNCSLFSPTPSLSAMVINKYKLRSNIKSFNLSGMGCSAGLISIDLARDLLQVYPNSNAVVVSTEIITPNYYQGKERAMLLPNCLFRMGGAAILLSNKKTEHKRAKYRLMQVVRTHKGGDDKAYRCVFEEEDREGKVGISLQKDLMAIAGEALKSNITTIGPLVLPASEQLLFLFTLIGKKIFNPKMKPYIPDFKQAFEHFCIHAGGRAVIDELQKNLQLSAEHVEASRMTLHRFGNTSSSSLWYELNYIESKGRMKRGDRVWQIAFGSGFKCNSAVWKCNRSIKTPVNGPWEDCIDRYPVHIPEIVKL*