Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027686.01.T01 | QZL13490.1 | 98.077 | 52 | 1 | 0 | 1 | 52 | 1319 | 1370 | 3.06E-25 | 107 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027686.01.T01 | P12227 | 82.692 | 52 | 8 | 1 | 1 | 52 | 1110 | 1160 | 4.01E-20 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027686.01.T01 | A0A4P8F181 | 98.077 | 52 | 1 | 0 | 1 | 52 | 1319 | 1370 | 1.50e-25 | 107 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013922.01 | MsG0580027686.01 | 0.848358 | 6.449138e-60 | 1.180438e-56 |
| MsG0580027686.01 | MsG0780037769.01 | 0.845454 | 4.020070e-59 | 6.702704e-56 |
| MsG0580027686.01 | MsG0780038139.01 | 0.801666 | 8.085434e-49 | 3.883540e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 2 sgRNAs with CRISPR-Local
Find 6 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATGATACCCGTTGGTAC+CGG | 0.541673 | 5:+66139620 | None:intergenic |
| TATATTGCGTTGACTGTGAA+CGG | 0.589124 | 5:-66139661 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATAATTTTTTTTTTTTCA+AGG | - | Chr5:66139696-66139715 | None:intergenic | 10.0% |
| !!! | AATCAAATAATTTTCTGTGA+TGG | - | Chr5:66139745-66139764 | None:intergenic | 20.0% |
| ! | GTGCATTATTCTTTTGAATC+CGG | - | Chr5:66139642-66139661 | None:intergenic | 30.0% |
| !! | TATATTGCGTTGACTGTGAA+CGG | - | Chr5:66139664-66139683 | None:intergenic | 35.0% |
| ! | TCTTTTGAATCCGGTACCAA+CGG | - | Chr5:66139633-66139652 | None:intergenic | 40.0% |
| ! | CTTTTGAATCCGGTACCAAC+GGG | - | Chr5:66139632-66139651 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 66139623 | 66139781 | 66139623 | ID=MsG0580027686.01;Name=MsG0580027686.01 |
| Chr5 | mRNA | 66139623 | 66139781 | 66139623 | ID=MsG0580027686.01.T01;Parent=MsG0580027686.01;Name=MsG0580027686.01.T01;_AED=0.29;_eAED=0.29;_QI=0|-1|0|1|-1|0|1|0|52 |
| Chr5 | exon | 66139623 | 66139781 | 66139623 | ID=MsG0580027686.01.T01:exon:34071;Parent=MsG0580027686.01.T01 |
| Chr5 | CDS | 66139623 | 66139781 | 66139623 | ID=MsG0580027686.01.T01:cds;Parent=MsG0580027686.01.T01 |
| Gene Sequence |
| Protein sequence |
