AlfalfaGEDB | MsG0780038139.01

M. sativa cultivar ZhongmuNo.1 / MsG0780038139.01

KEGG: K02963
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF01084

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780038139.01.T02	YP_009141632.1	88.71	62	7	0	1	62	33	94	3.79E-29	110
MsG0780038139.01.T01	YP_009141632.1	91.398	93	8	0	36	128	2	94	3.66E-51	168

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780038139.01.T01	P49169	77.174	92	21	0	36	127	2	93	4.67E-41	135
MsG0780038139.01.T02	P49169	75.41	61	15	0	1	61	33	93	2.18E-24	90.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780038139.01.T01	A0A0X8GKL0	91.398	93	8	0	36	128	2	94	1.75e-51	168
MsG0780038139.01.T02	A0A0F6NLD5	88.710	62	7	0	1	62	33	94	1.81e-29	110

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380013922.01	MsG0780038139.01	0.867706	1.122996e-65	3.957941e-62
MsG0580027686.01	MsG0780038139.01	0.801666	8.085434e-49	3.883540e-46
MsG0780037769.01	MsG0780038139.01	0.833867	4.165823e-56	4.859995e-53
MsG0780037869.01	MsG0780038139.01	0.853479	2.326267e-61	5.033196e-58
MsG0780038139.01	MsG0880044963.01	0.807650	4.557532e-50	2.552413e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780038139.01.T02	MTR_4g051350	91.304	46	4	0	1	46	118	163	2.35e-21	82.8
MsG0780038139.01.T01	MTR_4g051350	93.421	76	5	0	37	112	88	163	2.82e-41	136

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780038139.01.T02	ATCG00650	82.979	47	8	0	1	47	33	79	4.60e-20	76.6
MsG0780038139.01.T01	ATCG00650	79.747	79	16	0	36	114	2	80	4.76e-38	125

Find 13 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTATTAATATTACGTTGTT+GGG	0.345588	7:+41071676	MsG0780038139.01.T01:CDS
TATTACGTTGTTGGGAGAAC+GGG	0.346678	7:+41071684	MsG0780038139.01.T01:CDS
ATTTATTAATATTACGTTGT+TGG	0.373643	7:+41071675	MsG0780038139.01.T01:CDS
AGTTTGTCCCCGATCCAATC+GGG	0.455483	7:+41071763	MsG0780038139.01.T01:CDS
GAGTTTGTCCCCGATCCAAT+CGG	0.465376	7:+41071762	MsG0780038139.01.T01:CDS
AAAGGTGACGAATTAACAAA+AGG	0.521079	7:-41071937	None:intergenic
ACAAAGATAAAATACGAGCT+TGG	0.526187	7:-41071912	None:intergenic
ATATTACGTTGTTGGGAGAA+CGG	0.539020	7:+41071683	MsG0780038139.01.T01:CDS
TATCGACTTATTAGTCAACA+TGG	0.624658	7:+41071820	MsG0780038139.01.T01:CDS
ATTACGTTGTTGGGAGAACG+GGG	0.643445	7:+41071685	MsG0780038139.01.T01:CDS
GTTTGTCCCCGATCCAATCG+GGG	0.647747	7:+41071764	MsG0780038139.01.T01:CDS
TTTGTCCCCGATCCAATCGG+GGG	0.661952	7:+41071765	MsG0780038139.01.T01:CDS
AAATCAGAGTAATCACACAA+CGG	0.730942	7:-41071651	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTTCATTATTAACAAA+AGG	-	Chr7:41071958-41071977	None:intergenic	10.0%
!!	ATTTATTAATATTACGTTGT+TGG	+	Chr7:41071675-41071694	MsG0780038139.01.T01:CDS	15.0%
!!	TTTATTAATATTACGTTGTT+GGG	+	Chr7:41071676-41071695	MsG0780038139.01.T01:CDS	15.0%
!!!	AATTAATCGTTGTTGTTTTA+AGG	-	Chr7:41071878-41071897	None:intergenic	20.0%
!!	AATCTAAGCAATCTTTTTGT+AGG	+	Chr7:41071740-41071759	MsG0780038139.01.T01:CDS	25.0%
	AAAGGTGACGAATTAACAAA+AGG	-	Chr7:41071940-41071959	None:intergenic	30.0%
	AAATCAGAGTAATCACACAA+CGG	-	Chr7:41071654-41071673	None:intergenic	30.0%
	ACAAAGATAAAATACGAGCT+TGG	-	Chr7:41071915-41071934	None:intergenic	30.0%
!!	TATCGACTTATTAGTCAACA+TGG	+	Chr7:41071820-41071839	MsG0780038139.01.T01:CDS	30.0%
	ATATTACGTTGTTGGGAGAA+CGG	+	Chr7:41071683-41071702	MsG0780038139.01.T01:CDS	35.0%
	TATTACGTTGTTGGGAGAAC+GGG	+	Chr7:41071684-41071703	MsG0780038139.01.T01:CDS	40.0%
	AATCAATTCGATCCCCCGAT+TGG	-	Chr7:41071780-41071799	None:intergenic	45.0%
	ATTACGTTGTTGGGAGAACG+GGG	+	Chr7:41071685-41071704	MsG0780038139.01.T01:CDS	45.0%
	AGTTTGTCCCCGATCCAATC+GGG	+	Chr7:41071763-41071782	MsG0780038139.01.T01:CDS	50.0%
	ATTCGATCCCCCGATTGGAT+CGG	-	Chr7:41071775-41071794	None:intergenic	50.0%
	GAGTTTGTCCCCGATCCAAT+CGG	+	Chr7:41071762-41071781	MsG0780038139.01.T01:CDS	50.0%
	GTTTGTCCCCGATCCAATCG+GGG	+	Chr7:41071764-41071783	MsG0780038139.01.T01:CDS	55.0%
	TTCGATCCCCCGATTGGATC+GGG	-	Chr7:41071774-41071793	None:intergenic	55.0%
	TTTGTCCCCGATCCAATCGG+GGG	+	Chr7:41071765-41071784	MsG0780038139.01.T01:CDS	55.0%
	TCGATCCCCCGATTGGATCG+GGG	-	Chr7:41071773-41071792	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	41071610	41072026	41071610	ID=MsG0780038139.01;Name=MsG0780038139.01
Chr7	mRNA	41071610	41072026	41071610	ID=MsG0780038139.01.T01;Parent=MsG0780038139.01;Name=MsG0780038139.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|138
Chr7	exon	41071610	41072026	41071610	ID=MsG0780038139.01.T01:exon:8282;Parent=MsG0780038139.01.T01
Chr7	CDS	41071610	41072026	41071610	ID=MsG0780038139.01.T01:cds;Parent=MsG0780038139.01.T01
Chr7	mRNA	41071808	41072026	41071808	ID=MsG0780038139.01.T02;Parent=MsG0780038139.01;Name=MsG0780038139.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|72
Chr7	exon	41071808	41072026	41071808	ID=MsG0780038139.01.T02:exon:8283;Parent=MsG0780038139.01.T02
Chr7	CDS	41071808	41072026	41071808	ID=MsG0780038139.01.T02:cds;Parent=MsG0780038139.01.T02
Chr7	mRNA	41071982	41072026	41071982	ID=MsG0780038139.01.T03;Parent=MsG0780038139.01;Name=MsG0780038139.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|14
Chr7	exon	41071982	41072026	41071982	ID=MsG0780038139.01.T03:exon:8284;Parent=MsG0780038139.01.T03
Chr7	CDS	41071982	41072026	41071982	ID=MsG0780038139.01.T03:cds;Parent=MsG0780038139.01.T03
Chr7	mRNA	41071839	41071865	41071839	ID=MsG0780038139.01.T04;Parent=MsG0780038139.01;Name=MsG0780038139.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|8
Chr7	exon	41071839	41071865	41071839	ID=MsG0780038139.01.T04:exon:8285;Parent=MsG0780038139.01.T04
Chr7	CDS	41071839	41071865	41071839	ID=MsG0780038139.01.T04:cds;Parent=MsG0780038139.01.T04

Gene Sequence

>MsG0780038139.01.T04

ATGGAAAAATATTATCTAGACGCGTGA

>MsG0780038139.01.T03

ATGAAAAAAGCGAGTCGATCACTAGAACTCCTAACACTGTTCTAA

>MsG0780038139.01.T02

ATGAGTTTACTTTATCGACTTATTAGTCAACATGGAAAAATATTATCTAGACGCGTGAATAGATCAACCTTAAAACAACAACGATTAATTACGATTGCTATAAACCAAGCTCGTATTTTATCTTTGTTACCTTTTGTTAATTCGTCACCTTTTGTTAATAATGAAAAAAATAATATGAAAAAAGCGAGTCGATCACTAGAACTCCTAACACTGTTCTAA

>MsG0780038139.01.T01

CTATTTTCTATTTATGAAATTTTGATTAGCAGTGTGACACTCCGTTGTGTGATTACTCTGATTTTATTTATTAATATTACGTTGTTGGGAGAACGGGGTGTTACAGATAAATCTAAGCGACTCTTTCTTAAATCTAAGCAATCTTTTTGTAGGAGTTTGTCCCCGATCCAATCGGGGGATCGAATTGATTATAAAAACATGAGTTTACTTTATCGACTTATTAGTCAACATGGAAAAATATTATCTAGACGCGTGAATAGATCAACCTTAAAACAACAACGATTAATTACGATTGCTATAAACCAAGCTCGTATTTTATCTTTGTTACCTTTTGTTAATTCGTCACCTTTTGTTAATAATGAAAAAAATAATATGAAAAAAGCGAGTCGATCACTAGAACTCCTAACACTGTTCTAA

Protein sequence

>MsG0780038139.01.T04

MEKYYLDA*

>MsG0780038139.01.T03

MKKASRSLELLTLF*

>MsG0780038139.01.T02

MSLLYRLISQHGKILSRRVNRSTLKQQRLITIAINQARILSLLPFVNSSPFVNNEKNNMKKASRSLELLTLF*

>MsG0780038139.01.T01

LFSIYEILISSVTLRCVITLILFINITLLGERGVTDKSKRLFLKSKQSFCRSLSPIQSGDRIDYKNMSLLYRLISQHGKILSRRVNRSTLKQQRLITIAINQARILSLLPFVNSSPFVNNEKNNMKKASRSLELLTLF*