Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042922.01.T01 | XP_013444550.1 | 96.45 | 676 | 23 | 1 | 1 | 675 | 86 | 761 | 0 | 1339 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042922.01.T01 | A0A072TYE4 | 96.450 | 676 | 23 | 1 | 1 | 675 | 86 | 761 | 0.0 | 1339 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048887.01 | MsG0880042922.01 | 0.840900 | 6.582284e-58 | 9.511296e-55 |
| MsG0080048896.01 | MsG0880042922.01 | 0.803774 | 2.969533e-49 | 1.505185e-46 |
| MsG0080048969.01 | MsG0880042922.01 | 0.838128 | 3.457202e-57 | 4.586500e-54 |
| MsG0080048987.01 | MsG0880042922.01 | 0.809117 | 2.217349e-50 | 1.290796e-47 |
| MsG0080049023.01 | MsG0880042922.01 | 0.805610 | 1.228268e-49 | 6.526957e-47 |
| MsG0080049024.01 | MsG0880042922.01 | 0.818468 | 1.929955e-52 | 1.445934e-49 |
| MsG0180000017.01 | MsG0880042922.01 | 0.822420 | 2.392358e-53 | 2.001458e-50 |
| MsG0180000040.01 | MsG0880042922.01 | 0.877543 | 5.777315e-69 | 2.915623e-65 |
| MsG0180000490.01 | MsG0880042922.01 | 0.861390 | 1.061453e-63 | 2.998803e-60 |
| MsG0180000491.01 | MsG0880042922.01 | 0.846036 | 2.794975e-59 | 4.748827e-56 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042922.01.T01 | MTR_8g024380 | 96.450 | 676 | 23 | 1 | 1 | 675 | 86 | 761 | 0.0 | 1339 |
| MsG0880042922.01.T01 | MTR_1g078110 | 82.249 | 676 | 117 | 2 | 1 | 676 | 85 | 757 | 0.0 | 1155 |
| MsG0880042922.01.T01 | MTR_8g024420 | 70.545 | 679 | 191 | 6 | 1 | 672 | 45 | 721 | 0.0 | 964 |
| MsG0880042922.01.T01 | MTR_2g049200 | 72.206 | 680 | 178 | 7 | 1 | 672 | 79 | 755 | 0.0 | 964 |
| MsG0880042922.01.T01 | MTR_8g024390 | 70.044 | 681 | 193 | 7 | 1 | 672 | 79 | 757 | 0.0 | 947 |
| MsG0880042922.01.T01 | MTR_7g118080 | 68.041 | 679 | 208 | 6 | 1 | 675 | 85 | 758 | 0.0 | 921 |
| MsG0880042922.01.T01 | MTR_8g024370 | 68.850 | 687 | 194 | 9 | 1 | 672 | 75 | 756 | 0.0 | 915 |
| MsG0880042922.01.T01 | MTR_1g079120 | 52.117 | 685 | 310 | 11 | 1 | 673 | 83 | 761 | 0.0 | 682 |
| MsG0880042922.01.T01 | MTR_7g079310 | 50.437 | 686 | 320 | 11 | 1 | 673 | 86 | 764 | 0.0 | 678 |
| MsG0880042922.01.T01 | MTR_7g079300 | 50.436 | 688 | 316 | 13 | 1 | 673 | 89 | 766 | 0.0 | 668 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042922.01.T01 | AT5G67090 | 47.515 | 684 | 300 | 17 | 1 | 661 | 68 | 715 | 0.0 | 572 |
| MsG0880042922.01.T01 | AT4G34980 | 42.817 | 703 | 369 | 16 | 1 | 676 | 66 | 762 | 0.0 | 535 |
| MsG0880042922.01.T01 | AT2G05920 | 42.302 | 695 | 361 | 13 | 1 | 670 | 68 | 747 | 3.64e-174 | 516 |
| MsG0880042922.01.T01 | AT5G67360 | 42.796 | 701 | 355 | 12 | 1 | 673 | 73 | 755 | 5.74e-173 | 513 |
| MsG0880042922.01.T01 | AT3G14240 | 42.053 | 711 | 370 | 14 | 1 | 676 | 70 | 773 | 2.79e-170 | 506 |
| MsG0880042922.01.T01 | AT1G01900 | 43.777 | 699 | 352 | 14 | 1 | 672 | 87 | 771 | 3.09e-169 | 503 |
| MsG0880042922.01.T01 | AT1G04110 | 40.736 | 707 | 373 | 14 | 1 | 674 | 79 | 772 | 6.38e-168 | 500 |
| MsG0880042922.01.T01 | AT5G51750 | 40.143 | 700 | 385 | 13 | 1 | 675 | 87 | 777 | 2.37e-162 | 486 |
| MsG0880042922.01.T01 | AT3G14067 | 40.429 | 700 | 380 | 17 | 1 | 672 | 77 | 767 | 3.17e-162 | 486 |
| MsG0880042922.01.T01 | AT1G20160 | 40.423 | 710 | 364 | 18 | 1 | 673 | 36 | 723 | 2.34e-151 | 456 |
Find 143 sgRNAs with CRISPR-Local
Find 164 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGAAGAAAGTGGCTTT+TGG | 0.302465 | 8:+16028282 | MsG0880042922.01.T01:CDS |
| AAACCTGACATCACTGCTCC+TGG | 0.518027 | 8:+16027592 | MsG0880042922.01.T01:CDS |
| AAATATCCAAATATAACCTT+AGG | 0.419204 | 8:+16026698 | MsG0880042922.01.T01:CDS |
| AAATGTTCCTGTGATGGATT+TGG | 0.347365 | 8:+16027642 | MsG0880042922.01.T01:CDS |
| AACAAGTTCAATTTGATAAG+TGG | 0.388291 | 8:+16027682 | MsG0880042922.01.T01:CDS |
| AACACCTTGGGTCATAACTG+TGG | 0.724927 | 8:+16027090 | MsG0880042922.01.T01:CDS |
| AACAGTTACCAATGTTGGTG+AGG | 0.572151 | 8:+16028125 | MsG0880042922.01.T01:CDS |
| AACTCCACTCGTGATTCACA+TGG | 0.603671 | 8:+16026725 | MsG0880042922.01.T01:CDS |
| AACTGTGGCTGCTGGAACTA+TGG | 0.205089 | 8:+16027105 | MsG0880042922.01.T01:CDS |
| AACTGTTCTATGAAATTCTT+GGG | 0.410693 | 8:-16028109 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACGACAAAAATGAAGAAAG+TGG | + | Chr8:16028275-16028294 | MsG0880042922.01.T01:CDS | 30.0% | |
| ACTATGGATAGAGAATTTCA+AGG | + | Chr8:16027121-16027140 | MsG0880042922.01.T01:CDS | 30.0% | |
| ATCATTGTTGATCCAATCAA+TGG | + | Chr8:16027415-16027434 | MsG0880042922.01.T01:CDS | 30.0% | |
| ATGTCAGGTTTCAAAACATA+TGG | - | Chr8:16027583-16027602 | None:intergenic | 30.0% | |
| ATTGATTGGATCAACAATGA+TGG | - | Chr8:16027416-16027435 | None:intergenic | 30.0% | |
| CAATCCTTTGTTGAAGAATT+TGG | - | Chr8:16026672-16026691 | None:intergenic | 30.0% | |
| CATAAACCCATGAAAACAAT+TGG | - | Chr8:16027223-16027242 | None:intergenic | 30.0% | |
| CTATGGATAGAGAATTTCAA+GGG | + | Chr8:16027122-16027141 | MsG0880042922.01.T01:CDS | 30.0% | |
| GAAATTCCTTCAAAATGGAA+TGG | + | Chr8:16026587-16026606 | MsG0880042922.01.T01:CDS | 30.0% | |
| GAACTATAAGAAGAAACACT+AGG | - | Chr8:16027538-16027557 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 16026341 | 16028371 | 16026341 | ID=MsG0880042922.01;Name=MsG0880042922.01 |
| Chr8 | mRNA | 16026341 | 16028371 | 16026341 | ID=MsG0880042922.01.T01;Parent=MsG0880042922.01;Name=MsG0880042922.01.T01;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|676 |
| Chr8 | exon | 16026341 | 16028371 | 16026341 | ID=MsG0880042922.01.T01:exon:4220;Parent=MsG0880042922.01.T01 |
| Chr8 | CDS | 16026341 | 16028371 | 16026341 | ID=MsG0880042922.01.T01:cds;Parent=MsG0880042922.01.T01 |
| Gene Sequence |
>MsG0880042922.01.T01 ATGAATGGTTTTAGTGCTAATTTATCACCAAAAGAACATGAATCCCTCAAAACTTCACCAGGCTACATTTCATCCATAAAAGATTCACACATGAAACTCGACACAACACATTCACCTCAGTTCCTCGGCCTCAATCCCAACAAAGGCGCTTGGCATGATTCGAATTTTGGCAACGACGTAATTGTTGGTTTAATCGACACCGGAATTTGGCCCGAAAGCGAAAGCTTTAAAGATAATTTAATGTCCGAAATTCCTTCAAAATGGAATGGACAGTGTGAAAATAGCATTCTTTTCAATTCATCTCTTTGCAACAAGAAACTCATTGGTGCCAAATTCTTCAACAAAGGATTGCGATCCAAATATCCAAATATAACCTTAGGTTTGAACTCCACTCGTGATTCACATGGCCATGGGACTCACACTTCATCAACAGCAGCTGGAAACAGAGTTGATGATGCTTCATTTTTTGGTTATGCACCTGGAACAGCTTCAGGAATAGCTTCAAATTCACGAGTAGCAATGTATAAAGCAATATGGGATGTTGGATTTTTATCATCTGATGTAATAGCCGCAATTGACGCGGCAATATCAGACGGTGTTGATGTTCTTTCACTATCATTTGGTAAAAATGACGTGCCTTTGTATGACGATCCTGTTGCCATAGCAACATTTGCAGCTATGGAAAAAGGTGTTTTTGTTTCCACTTCAGCTGGTAATAGTGGTCCTGCCCTTGAAACTCTTCACAATGGAACACCTTGGGTCATAACTGTGGCTGCTGGAACTATGGATAGAGAATTTCAAGGGACCCTTACACTTGGAAATGGTAACACAATCATGGGGTTGTCACTTTATATAGGAACATTTTCTTCCAATAATGTTCCAATTGTTTTCATGGGTTTATGTGACAATGTTAAGGAATTGAAAAAAGTGAAAAACAAGATTGTTGTGTGTGAAGACAAGGATGGAGCAAGCCTTTTTTATCAAGTCGATAATTTGGATGAAGCAAAAGTTCTTGGGGCTGTTTTTATTACAAATGTTACAGACATGTACTATTCAGAAAATAGTTTTGCATCCATCATTGTTGATCCAATCAATGGAGAAGCAGTCAAAGCTTACATCAAGAGTTACTCTAAAAACTCTACTTCAAAAGCAAGCATGTCTTTCATGAAAACAGTTTTTGGAATTAAACCAGCACCTAGTGTTTCTTCTTATAGTTCAAGAGGGCCTTCAAATAGCTGTCCATATGTTTTGAAACCTGACATCACTGCTCCTGGTACAAATATCTTAGCTGCATGGCCCACAAATGTTCCTGTGATGGATTTGGGGAATCATAAGATCTTCAACAAGTTCAATTTGATAAGTGGAACATCTATGGCATGTCCTCATGTTGCAGGTGTCGCGGCACTTTTGAAAGGAGCACACGGTGATTGGAGCCCTGCCGCGATTCGGTCGGCAATTATGACAACGTCGGACATATTTGATAATACTAAGGAGCATATTAAAGACATTGGAAATGATAATAAAGCAGCAACTCCTTTTGCATTGGGAGCTGGTCATGTTAATCCAAATAGAGCACTTGACCCTGGTCTTATTTACGATGTTGGTGTACAAGATTATGTTAATCTTCTTTGTGCACTTAACTACTCACAGAAAAACATCACTGCCATCACAAGATCTTCTTCGAATGATTGCTCTGAACCTTCTTTGGATCTTAACTACCCTTCTTTTATTGCATATTTCAATAATGGAAATTCTTCTTCAAGGACAACCCAAGAATTTCATAGAACAGTTACCAATGTTGGTGAGGGAGAAACAATTTATGTTTCTAGCATTACTCAGGTTGAAGGGTTCCGTGTCAGTGTTATCCCAAACAAGTTGGTATTCACTGAGAAGAATGAGAAACTAAGCTACAAGTTGAGGATTGAAGTTGCAAGAACGACAAAAATGAAGAAAGTGGCTTTTGGGTATCTAACTTGGATGGATATGAAGCATGTGGTTAGAAGTCCAATCGTGGTCGCAATACTCGAATAG |
| Protein sequence |
>MsG0880042922.01.T01 MNGFSANLSPKEHESLKTSPGYISSIKDSHMKLDTTHSPQFLGLNPNKGAWHDSNFGNDVIVGLIDTGIWPESESFKDNLMSEIPSKWNGQCENSILFNSSLCNKKLIGAKFFNKGLRSKYPNITLGLNSTRDSHGHGTHTSSTAAGNRVDDASFFGYAPGTASGIASNSRVAMYKAIWDVGFLSSDVIAAIDAAISDGVDVLSLSFGKNDVPLYDDPVAIATFAAMEKGVFVSTSAGNSGPALETLHNGTPWVITVAAGTMDREFQGTLTLGNGNTIMGLSLYIGTFSSNNVPIVFMGLCDNVKELKKVKNKIVVCEDKDGASLFYQVDNLDEAKVLGAVFITNVTDMYYSENSFASIIVDPINGEAVKAYIKSYSKNSTSKASMSFMKTVFGIKPAPSVSSYSSRGPSNSCPYVLKPDITAPGTNILAAWPTNVPVMDLGNHKIFNKFNLISGTSMACPHVAGVAALLKGAHGDWSPAAIRSAIMTTSDIFDNTKEHIKDIGNDNKAATPFALGAGHVNPNRALDPGLIYDVGVQDYVNLLCALNYSQKNITAITRSSSNDCSEPSLDLNYPSFIAYFNNGNSSSRTTQEFHRTVTNVGEGETIYVSSITQVEGFRVSVIPNKLVFTEKNEKLSYKLRIEVARTTKMKKVAFGYLTWMDMKHVVRSPIVVAILE* |
