Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027948.01.T01 | KEH24808.1 | 95 | 80 | 4 | 0 | 1 | 80 | 296 | 375 | 2.88E-40 | 152 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027948.01.T01 | A0A072U6P8 | 95.000 | 80 | 4 | 0 | 1 | 80 | 296 | 375 | 1.38e-40 | 152 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000470.01 | MsG0580027948.01 | 0.826398 | 2.772928e-54 | 2.597590e-51 |
| MsG0280010722.01 | MsG0580027948.01 | 0.822165 | 2.741599e-53 | 2.276793e-50 |
| MsG0380011609.01 | MsG0580027948.01 | 0.819289 | 1.255968e-52 | 9.624437e-50 |
| MsG0380012460.01 | MsG0580027948.01 | 0.801114 | 1.048940e-48 | 4.968718e-46 |
| MsG0580027947.01 | MsG0580027948.01 | 0.831878 | 1.300315e-55 | 1.429784e-52 |
| MsG0280007955.01 | MsG0580027948.01 | 0.824063 | 9.889389e-54 | 8.666285e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027948.01.T01 | MTR_6g005840 | 95.000 | 80 | 4 | 0 | 1 | 80 | 296 | 375 | 3.49e-44 | 152 |
| MsG0580027948.01.T01 | MTR_6g005840 | 95.000 | 80 | 4 | 0 | 1 | 80 | 338 | 417 | 3.61e-44 | 152 |
| MsG0580027948.01.T01 | MTR_6g005960 | 87.500 | 80 | 10 | 0 | 1 | 80 | 278 | 357 | 9.20e-41 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCTCTCTGTGCCACTATC+TGG | 0.338217 | 5:-71627551 | None:intergenic |
| AGTCGTTCGTTTGTTGTTGC+GGG | 0.355271 | 5:+71627461 | MsG0580027948.01.T01:CDS |
| AAATGAGGTTGGAAGGGTTC+TGG | 0.372760 | 5:+71627427 | MsG0580027948.01.T01:CDS |
| AACAGCATAGGTAAAAGCTT+TGG | 0.424351 | 5:-71627393 | None:intergenic |
| TTCGGCGGTTACACTTGAAC+CGG | 0.441099 | 5:+71627574 | MsG0580027948.01.T01:CDS |
| TAGTGGCACAGAGAGCATTT+CGG | 0.477803 | 5:+71627556 | MsG0580027948.01.T01:CDS |
| CAAGAAGCTGTAAATGAGGT+TGG | 0.503519 | 5:+71627416 | MsG0580027948.01.T01:CDS |
| GAGTCGTTCGTTTGTTGTTG+CGG | 0.521739 | 5:+71627460 | MsG0580027948.01.T01:CDS |
| GTCTGAATTCGCTCCAATGT+TGG | 0.525621 | 5:+71627493 | MsG0580027948.01.T01:CDS |
| AAGCTGTAAATGAGGTTGGA+AGG | 0.558828 | 5:+71627420 | MsG0580027948.01.T01:CDS |
| CCGGCTGAGTTACTTTCTCA+AGG | 0.559387 | 5:+71627593 | MsG0580027948.01.T01:CDS |
| CCTTGAGAAAGTAACTCAGC+CGG | 0.559575 | 5:-71627593 | None:intergenic |
| GTCGTTCGTTTGTTGTTGCG+GGG | 0.581074 | 5:+71627462 | MsG0580027948.01.T01:CDS |
| AGCTGTAAATGAGGTTGGAA+GGG | 0.590294 | 5:+71627421 | MsG0580027948.01.T01:CDS |
| TACAGCTTCTTGAACAGCAT+AGG | 0.602664 | 5:-71627405 | None:intergenic |
| TCGTTCGTTTGTTGTTGCGG+GGG | 0.630767 | 5:+71627463 | MsG0580027948.01.T01:CDS |
| TGTTCAAGAAGCTGTAAATG+AGG | 0.656142 | 5:+71627412 | MsG0580027948.01.T01:CDS |
| TTTGATGATATGGTGAGACG+AGG | 0.681159 | 5:+71627365 | None:intergenic |
| TGGCACAGAGAGCATTTCGG+CGG | 0.682197 | 5:+71627559 | MsG0580027948.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATTCAGGAATCAAGGA+AGG | + | Chr5:71627518-71627537 | MsG0580027948.01.T01:CDS | 30.0% | |
| AACAGCATAGGTAAAAGCTT+TGG | - | Chr5:71627396-71627415 | None:intergenic | 35.0% | |
| TGTTCAAGAAGCTGTAAATG+AGG | + | Chr5:71627412-71627431 | MsG0580027948.01.T01:CDS | 35.0% | |
| AAGCTGTAAATGAGGTTGGA+AGG | + | Chr5:71627420-71627439 | MsG0580027948.01.T01:CDS | 40.0% | |
| AGCTGTAAATGAGGTTGGAA+GGG | + | Chr5:71627421-71627440 | MsG0580027948.01.T01:CDS | 40.0% | |
| CAAGAAGCTGTAAATGAGGT+TGG | + | Chr5:71627416-71627435 | MsG0580027948.01.T01:CDS | 40.0% | |
| CCAATGTTGGCGAAAAATTC+AGG | + | Chr5:71627506-71627525 | MsG0580027948.01.T01:CDS | 40.0% | |
| GGCGAAAAATTCAGGAATCA+AGG | + | Chr5:71627514-71627533 | MsG0580027948.01.T01:CDS | 40.0% | |
| TACAGCTTCTTGAACAGCAT+AGG | - | Chr5:71627408-71627427 | None:intergenic | 40.0% | |
| ! | CCTGAATTTTTCGCCAACAT+TGG | - | Chr5:71627509-71627528 | None:intergenic | 40.0% |
| AAATGAGGTTGGAAGGGTTC+TGG | + | Chr5:71627427-71627446 | MsG0580027948.01.T01:CDS | 45.0% | |
| CCTTGAGAAAGTAACTCAGC+CGG | - | Chr5:71627596-71627615 | None:intergenic | 45.0% | |
| GTCTGAATTCGCTCCAATGT+TGG | + | Chr5:71627493-71627512 | MsG0580027948.01.T01:CDS | 45.0% | |
| !! | AGTCGTTCGTTTGTTGTTGC+GGG | + | Chr5:71627461-71627480 | MsG0580027948.01.T01:CDS | 45.0% |
| !! | GAGTCGTTCGTTTGTTGTTG+CGG | + | Chr5:71627460-71627479 | MsG0580027948.01.T01:CDS | 45.0% |
| !! | GGTGTTTTTGTGCCAGATAG+TGG | + | Chr5:71627539-71627558 | MsG0580027948.01.T01:CDS | 45.0% |
| !! | TAGTGGCACAGAGAGCATTT+CGG | + | Chr5:71627556-71627575 | MsG0580027948.01.T01:CDS | 45.0% |
| TTCGGCGGTTACACTTGAAC+CGG | + | Chr5:71627574-71627593 | MsG0580027948.01.T01:CDS | 50.0% | |
| ! | ATGCTCTCTGTGCCACTATC+TGG | - | Chr5:71627554-71627573 | None:intergenic | 50.0% |
| ! | CCGGCTGAGTTACTTTCTCA+AGG | + | Chr5:71627593-71627612 | MsG0580027948.01.T01:CDS | 50.0% |
| !! | GTCGTTCGTTTGTTGTTGCG+GGG | + | Chr5:71627462-71627481 | MsG0580027948.01.T01:CDS | 50.0% |
| !! | TCGTTCGTTTGTTGTTGCGG+GGG | + | Chr5:71627463-71627482 | MsG0580027948.01.T01:CDS | 50.0% |
| !! | TGGCACAGAGAGCATTTCGG+CGG | + | Chr5:71627559-71627578 | MsG0580027948.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 71627374 | 71627652 | 71627374 | ID=MsG0580027948.01;Name=MsG0580027948.01 |
| Chr5 | mRNA | 71627374 | 71627652 | 71627374 | ID=MsG0580027948.01.T01;Parent=MsG0580027948.01;Name=MsG0580027948.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|92 |
| Chr5 | exon | 71627374 | 71627652 | 71627374 | ID=MsG0580027948.01.T01:exon:4996;Parent=MsG0580027948.01.T01 |
| Chr5 | CDS | 71627374 | 71627652 | 71627374 | ID=MsG0580027948.01.T01:cds;Parent=MsG0580027948.01.T01 |
| Gene Sequence |
| Protein sequence |
