AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028775.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028775.01.T01 MTR_5g076270 100.000 75 0 0 9 83 312 386 4.05e-48 162
MsG0580028775.01.T01 MTR_3g064050 93.333 75 5 0 9 83 77 151 3.49e-45 153
MsG0580028775.01.T01 MTR_3g064050 93.333 75 5 0 9 83 314 388 1.86e-44 152
MsG0580028775.01.T01 MTR_8g079492 89.474 76 8 0 9 84 228 303 2.29e-41 143
MsG0580028775.01.T01 MTR_8g079492 89.474 76 8 0 9 84 310 385 2.62e-41 143
MsG0580028775.01.T01 MTR_8g079492 90.541 74 7 0 9 82 310 383 7.79e-41 142
MsG0580028775.01.T01 MTR_2g018690 88.158 76 9 0 9 84 311 386 1.60e-40 141
MsG0580028775.01.T01 MTR_4g124900 63.014 73 21 3 9 78 309 378 2.62e-23 92.4
MsG0580028775.01.T01 MTR_4g124900 63.014 73 21 3 9 78 309 378 2.67e-23 92.4
MsG0580028775.01.T01 MTR_2g043250 72.727 55 14 1 9 63 309 362 3.83e-22 89.0
MsG0580028775.01.T01 MTR_8g101360 66.667 51 17 0 9 59 319 369 9.89e-21 85.1
MsG0580028775.01.T01 MTR_1g024025 52.857 70 32 1 9 78 330 398 5.12e-19 80.1
MsG0580028775.01.T01 MTR_2g014770 46.154 65 35 0 9 73 341 405 1.55e-12 61.6
MsG0580028775.01.T01 MTR_2g014770 46.154 65 35 0 9 73 341 405 1.69e-12 61.6
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 355 416 3.91e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 368 429 3.91e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 355 416 4.04e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 368 429 4.05e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 355 416 4.10e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 368 429 4.18e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 238 299 4.35e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 238 299 4.41e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 225 286 4.45e-12 60.5
MsG0580028775.01.T01 MTR_2g093740 45.161 62 34 0 12 73 225 286 4.52e-12 60.5
MsG0580028775.01.T01 MTR_8g100050 47.826 69 33 1 9 77 345 410 1.58e-11 58.9
MsG0580028775.01.T01 MTR_8g100050 47.826 69 33 1 9 77 345 410 2.01e-11 58.5
MsG0580028775.01.T01 MTR_4g088210 47.692 65 33 1 9 73 331 394 3.62e-11 57.8
MsG0580028775.01.T01 MTR_4g088210 47.692 65 33 1 9 73 331 394 3.62e-11 57.8
MsG0580028775.01.T01 MTR_4g021580 45.588 68 34 2 9 74 304 370 9.29e-11 56.6
MsG0580028775.01.T01 MTR_4g021580 45.588 68 34 2 9 74 304 370 9.34e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028775.01.T01 AT5G37020 93.243 74 5 0 9 82 310 383 9.58e-44 150
MsG0580028775.01.T01 AT5G37020 93.243 74 5 0 9 82 215 288 9.85e-44 150
MsG0580028775.01.T01 AT5G37020 93.243 74 5 0 9 82 310 383 1.28e-43 150
MsG0580028775.01.T01 AT1G30330 86.842 76 10 0 9 84 313 388 4.75e-40 140
MsG0580028775.01.T01 AT1G30330 86.842 76 10 0 9 84 313 388 4.75e-40 140
MsG0580028775.01.T01 AT1G30330 86.842 76 10 0 9 84 311 386 4.99e-40 140
MsG0580028775.01.T01 AT1G19220 61.644 73 22 3 9 78 309 378 1.27e-21 87.4
MsG0580028775.01.T01 AT5G20730 65.455 55 18 1 9 63 310 363 1.82e-20 84.3
MsG0580028775.01.T01 AT5G20730 65.455 55 18 1 9 63 311 364 1.86e-20 84.3
MsG0580028775.01.T01 AT5G20730 65.455 55 18 1 9 63 311 364 1.95e-20 84.3
MsG0580028775.01.T01 AT1G19850 50.725 69 33 1 9 77 331 398 4.84e-16 71.6
MsG0580028775.01.T01 AT1G19850 50.725 69 33 1 9 77 342 409 4.94e-16 71.6
MsG0580028775.01.T01 AT5G60450 47.761 67 35 0 9 75 335 401 2.51e-14 66.6
MsG0580028775.01.T01 AT5G60450 47.761 67 35 0 9 75 359 425 2.80e-14 66.6
MsG0580028775.01.T01 AT5G62000 46.377 69 34 1 9 77 347 412 2.09e-12 61.2
MsG0580028775.01.T01 AT5G62000 46.377 69 34 1 9 77 347 412 2.09e-12 61.2
MsG0580028775.01.T01 AT5G62000 46.377 69 34 1 9 77 347 412 2.09e-12 61.2
MsG0580028775.01.T01 AT5G62000 46.377 69 34 1 9 77 347 412 2.09e-12 61.2
MsG0580028775.01.T01 AT5G62000 46.377 69 34 1 9 77 347 412 2.40e-12 61.2
MsG0580028775.01.T01 AT2G33860 46.774 62 33 0 12 73 188 249 2.28e-11 58.5
MsG0580028775.01.T01 AT2G33860 57.778 45 19 0 12 56 345 389 2.47e-11 58.2

Find 33 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAGACCTCCAATGAGAATT+TGG 0.247430 5:+86109634 None:intergenic
GCCATGGTCGTTTCAATCTA+AGG 0.278500 5:+86108985 None:intergenic
TACATTGGAAATGTAGTTAA+AGG 0.316141 5:+86109023 None:intergenic
GACAGCCACGGGTATCGTTA+TGG 0.368607 5:-86109055 MsG0580028775.01.T01:CDS
TCCATTGTGCATAGGTTGGT+TGG 0.395710 5:-86109100 MsG0580028775.01.T01:intron
TTCTCATTGGAGGTCTGTTA+AGG 0.408471 5:-86109629 MsG0580028775.01.T01:intron
TGAGAATTTGGCCACCGAAC+AGG 0.420532 5:+86109646 None:intergenic
ACCTTGTTTGTGCAGGTACA+TGG 0.430262 5:-86109698 MsG0580028775.01.T01:CDS
TTCGGTGGCCAAATTCTCAT+TGG 0.448662 5:-86109642 MsG0580028775.01.T01:CDS
CCATTGTGCATAGGTTGGTT+GGG 0.449329 5:-86109099 MsG0580028775.01.T01:intron
ACAGCCACGGGTATCGTTAT+GGG 0.450191 5:-86109054 MsG0580028775.01.T01:CDS
GGTTGGGATGAATCGACAGC+TGG 0.467846 5:-86109083 MsG0580028775.01.T01:CDS
GGAAATAGTGATGGATACAT+TGG 0.468477 5:+86109008 None:intergenic
CCATGGTCGTTTCAATCTAA+GGG 0.508697 5:+86108986 None:intergenic
GGTGGCCAAATTCTCATTGG+AGG 0.509028 5:-86109639 MsG0580028775.01.T01:CDS
TTGAAACGACCATGGCATCC+TGG 0.526294 5:-86108978 MsG0580028775.01.T01:CDS
CCAAGAAAAGAAGAAGTGCC+AGG 0.532299 5:+86108960 None:intergenic
CCTTGTTTGTGCAGGTACAT+GGG 0.538001 5:-86109697 MsG0580028775.01.T01:CDS
ATGTGCCACCTTGTTTGTGC+AGG 0.550647 5:-86109705 MsG0580028775.01.T01:CDS
TAAGTGACATGGATCCTGTT+CGG 0.554501 5:-86109660 MsG0580028775.01.T01:CDS
AATCTAAGGGGAAATAGTGA+TGG 0.562581 5:+86108999 None:intergenic
CCCATGTACCTGCACAAACA+AGG 0.564028 5:+86109697 None:intergenic
GTTGGGATGAATCGACAGCT+GGG 0.567928 5:-86109082 MsG0580028775.01.T01:CDS
GAAGAAGTGCCAGGATGCCA+TGG 0.571373 5:+86108969 None:intergenic
CCCTTAGATTGAAACGACCA+TGG 0.577662 5:-86108986 MsG0580028775.01.T01:CDS
AGCTGGGGAGAGACAGCCAC+GGG 0.580172 5:-86109066 MsG0580028775.01.T01:CDS
CATGGTCGTTTCAATCTAAG+GGG 0.661723 5:+86108987 None:intergenic
TATAACTAGCATAAGTGACA+TGG 0.663626 5:-86109671 MsG0580028775.01.T01:CDS
ATTTCCCATAACGATACCCG+TGG 0.672203 5:+86109050 None:intergenic
CAGCTGGGGAGAGACAGCCA+CGG 0.687247 5:-86109067 MsG0580028775.01.T01:CDS
GTGACATGGATCCTGTTCGG+TGG 0.692792 5:-86109657 MsG0580028775.01.T01:CDS
TTGGGATGAATCGACAGCTG+GGG 0.697786 5:-86109081 MsG0580028775.01.T01:CDS
ATGTACCTGCACAAACAAGG+TGG 0.738221 5:+86109700 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCATTAAAAGTTTTGAA+AGG - Chr5:86109510-86109529 MsG0580028775.01.T01:intron 15.0%
!! AAATATCTACTTAATCGAAA+AGG + Chr5:86109349-86109368 None:intergenic 20.0%
!! CACAATGTAATACATAAAAT+TGG - Chr5:86109063-86109082 MsG0580028775.01.T01:CDS 20.0%
!! TTGTATTCCATATATTAGAA+AGG - Chr5:86109478-86109497 MsG0580028775.01.T01:intron 20.0%
!!! AGATTTCAAGTGTTTTTTAA+AGG + Chr5:86109217-86109236 None:intergenic 20.0%
!!! TATTTTCTTTTTACATAACC+TGG - Chr5:86109364-86109383 MsG0580028775.01.T01:intron 20.0%
! AAGGTTTATTAATCAAAGAC+AGG + Chr5:86109198-86109217 None:intergenic 25.0%
! ACTAAGACCTTTCTAATATA+TGG + Chr5:86109488-86109507 None:intergenic 25.0%
! CAAAGTAAACTTTATGAAAC+AGG + Chr5:86109245-86109264 None:intergenic 25.0%
! GAATCATGTTCAAATTCAAT+AGG - Chr5:86109400-86109419 MsG0580028775.01.T01:intron 25.0%
! TACATTGGAAATGTAGTTAA+AGG + Chr5:86109636-86109655 None:intergenic 25.0%
AAGGGAGAATGTTAATACAA+CGG - Chr5:86109127-86109146 MsG0580028775.01.T01:intron 30.0%
ATGGATGAAACAAGATAATC+AGG + Chr5:86109541-86109560 None:intergenic 30.0%
TATAACTAGCATAAGTGACA+TGG - Chr5:86108985-86109004 MsG0580028775.01.T01:CDS 30.0%
! TTGAGTTCTAATCATGAAGA+GGG - Chr5:86109171-86109190 MsG0580028775.01.T01:intron 30.0%
! TTTGAGTTCTAATCATGAAG+AGG - Chr5:86109170-86109189 MsG0580028775.01.T01:intron 30.0%
!! CTTCTTGACTAGGTTTTAAT+CGG - Chr5:86109436-86109455 MsG0580028775.01.T01:intron 30.0%
AATCTAAGGGGAAATAGTGA+TGG + Chr5:86109660-86109679 None:intergenic 35.0%
CCAAAAAACCTCATGGTTTA+AGG + Chr5:86109281-86109300 None:intergenic 35.0%
CCTTCGAAACATAAGACTAA+GGG - Chr5:86109109-86109128 MsG0580028775.01.T01:intron 35.0%
CGATTAAAACCTAGTCAAGA+AGG + Chr5:86109438-86109457 None:intergenic 35.0%
CTCATGGTTTAAGGATGAAA+AGG + Chr5:86109272-86109291 None:intergenic 35.0%
TCCTTCGAAACATAAGACTA+AGG - Chr5:86109108-86109127 MsG0580028775.01.T01:intron 35.0%
TTGTTTCATCCATTGTGCAT+AGG - Chr5:86109548-86109567 MsG0580028775.01.T01:intron 35.0%
! CTTTTCATCCTTAAACCATG+AGG - Chr5:86109270-86109289 MsG0580028775.01.T01:intron 35.0%
! TGAGTTCTAATCATGAAGAG+GGG - Chr5:86109172-86109191 MsG0580028775.01.T01:intron 35.0%
! TTTAGTTCACCTTCTTGACT+AGG - Chr5:86109426-86109445 MsG0580028775.01.T01:intron 35.0%
!! ACTAGGTTTTAATCGGTTCA+AGG - Chr5:86109443-86109462 MsG0580028775.01.T01:intron 35.0%
!! GGAAATAGTGATGGATACAT+TGG + Chr5:86109651-86109670 None:intergenic 35.0%
!!! AACGGTAACTTTGCTGTTTT+GGG - Chr5:86109145-86109164 MsG0580028775.01.T01:intron 35.0%
!!! CCTTAAACCATGAGGTTTTT+TGG - Chr5:86109278-86109297 MsG0580028775.01.T01:intron 35.0%
ACAGACCTCCAATGAGAATT+TGG + Chr5:86109025-86109044 None:intergenic 40.0%
CATGGTCGTTTCAATCTAAG+GGG + Chr5:86109672-86109691 None:intergenic 40.0%
CCATGGTCGTTTCAATCTAA+GGG + Chr5:86109673-86109692 None:intergenic 40.0%
GACTACTCCAAAAAACCTCA+TGG + Chr5:86109288-86109307 None:intergenic 40.0%
TAAGTGACATGGATCCTGTT+CGG - Chr5:86108996-86109015 MsG0580028775.01.T01:CDS 40.0%
TTCATCCATTGTGCATAGGT+TGG - Chr5:86109552-86109571 MsG0580028775.01.T01:intron 40.0%
TTCTCATTGGAGGTCTGTTA+AGG - Chr5:86109027-86109046 MsG0580028775.01.T01:CDS 40.0%
!! CCCTTAGTCTTATGTTTCGA+AGG + Chr5:86109112-86109131 None:intergenic 40.0%
!!! CAACGGTAACTTTGCTGTTT+TGG - Chr5:86109144-86109163 MsG0580028775.01.T01:intron 40.0%
!!! TGAGGTTTTTTGGAGTAGTC+TGG - Chr5:86109288-86109307 MsG0580028775.01.T01:intron 40.0%
ATGTACCTGCACAAACAAGG+TGG + Chr5:86108959-86108978 None:intergenic 45.0%
ATTTCCCATAACGATACCCG+TGG + Chr5:86109609-86109628 None:intergenic 45.0%
CCCTTAGATTGAAACGACCA+TGG - Chr5:86109670-86109689 MsG0580028775.01.T01:CDS 45.0%
GCCATGGTCGTTTCAATCTA+AGG + Chr5:86109674-86109693 None:intergenic 45.0%
TTCGGTGGCCAAATTCTCAT+TGG - Chr5:86109014-86109033 MsG0580028775.01.T01:CDS 45.0%
! ACCTTGTTTGTGCAGGTACA+TGG - Chr5:86108958-86108977 MsG0580028775.01.T01:CDS 45.0%
! CCTGGCACTTCTTCTTTTCT+TGG - Chr5:86109696-86109715 MsG0580028775.01.T01:CDS 45.0%
! CCTTGTTTGTGCAGGTACAT+GGG - Chr5:86108959-86108978 MsG0580028775.01.T01:CDS 45.0%
! GATTCGTGTTGCTGTATTCC+AGG + Chr5:86109385-86109404 None:intergenic 45.0%
!! CCAAGAAAAGAAGAAGTGCC+AGG + Chr5:86109699-86109718 None:intergenic 45.0%
!! CCATTGTGCATAGGTTGGTT+GGG - Chr5:86109557-86109576 MsG0580028775.01.T01:intron 45.0%
!! TCCATTGTGCATAGGTTGGT+TGG - Chr5:86109556-86109575 MsG0580028775.01.T01:intron 45.0%
CCCAACCAACCTATGCACAA+TGG + Chr5:86109560-86109579 None:intergenic 50.0%
CCCATGTACCTGCACAAACA+AGG + Chr5:86108962-86108981 None:intergenic 50.0%
GGTGGCCAAATTCTCATTGG+AGG - Chr5:86109017-86109036 MsG0580028775.01.T01:CDS 50.0%
GTTGGGATGAATCGACAGCT+GGG - Chr5:86109574-86109593 MsG0580028775.01.T01:intron 50.0%
TGAGAATTTGGCCACCGAAC+AGG + Chr5:86109013-86109032 None:intergenic 50.0%
TTGAAACGACCATGGCATCC+TGG - Chr5:86109678-86109697 MsG0580028775.01.T01:CDS 50.0%
TTGGGATGAATCGACAGCTG+GGG - Chr5:86109575-86109594 MsG0580028775.01.T01:intron 50.0%
! ACAGCCACGGGTATCGTTAT+GGG - Chr5:86109602-86109621 MsG0580028775.01.T01:intron 50.0%
! ATGTGCCACCTTGTTTGTGC+AGG - Chr5:86108951-86108970 MsG0580028775.01.T01:CDS 50.0%
GGTTGGGATGAATCGACAGC+TGG - Chr5:86109573-86109592 MsG0580028775.01.T01:intron 55.0%
GTGACATGGATCCTGTTCGG+TGG - Chr5:86108999-86109018 MsG0580028775.01.T01:CDS 55.0%
! GACAGCCACGGGTATCGTTA+TGG - Chr5:86109601-86109620 MsG0580028775.01.T01:intron 55.0%
!! GAAGAAGTGCCAGGATGCCA+TGG + Chr5:86109690-86109709 None:intergenic 55.0%
AGCTGGGGAGAGACAGCCAC+GGG - Chr5:86109590-86109609 MsG0580028775.01.T01:intron 65.0%
CAGCTGGGGAGAGACAGCCA+CGG - Chr5:86109589-86109608 MsG0580028775.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 86108947 86109731 86108947 ID=MsG0580028775.01;Name=MsG0580028775.01
Chr5 mRNA 86108947 86109731 86108947 ID=MsG0580028775.01.T01;Parent=MsG0580028775.01;Name=MsG0580028775.01.T01;_AED=0.49;_eAED=0.56;_QI=0|1|0|1|1|1|2|0|87
Chr5 exon 86109630 86109731 86109630 ID=MsG0580028775.01.T01:exon:21678;Parent=MsG0580028775.01.T01
Chr5 exon 86108947 86109108 86108947 ID=MsG0580028775.01.T01:exon:21677;Parent=MsG0580028775.01.T01
Chr5 CDS 86109630 86109731 86109630 ID=MsG0580028775.01.T01:cds;Parent=MsG0580028775.01.T01
Chr5 CDS 86108947 86109108 86108947 ID=MsG0580028775.01.T01:cds;Parent=MsG0580028775.01.T01
Gene Sequence

>MsG0580028775.01.T01

ATGCATGTGCCACCTTGTTTGTGCAGGTACATGGGTACTATAACTAGCATAAGTGACATGGATCCTGTTCGGTGGCCAAATTCTCATTGGAGGTCTGTTAAGGTTGGTTGGGATGAATCGACAGCTGGGGAGAGACAGCCACGGGTATCGTTATGGGAAATTGAGCCTTTAACTACATTTCCAATGTATCCATCACTATTTCCCCTTAGATTGAAACGACCATGGCATCCTGGCACTTCTTCTTTTCTTGGTACATATTTTTAA

Protein sequence

>MsG0580028775.01.T01

MHVPPCLCRYMGTITSISDMDPVRWPNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTFPMYPSLFPLRLKRPWHPGTSSFLGTYF*