AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029531.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029531.01.T01 MTR_5g087960 86.667 165 3 2 1 147 1 164 3.95e-98 279
MsG0580029531.01.T01 MTR_5g087980 73.171 41 11 0 34 74 18 58 1.75e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029531.01.T01 AT5G60335 51.020 147 52 3 15 141 12 158 1.44e-44 144
MsG0580029531.01.T01 AT5G60335 51.020 147 52 3 15 141 12 158 1.44e-44 144
MsG0580029531.01.T01 AT5G60335 51.020 147 52 3 15 141 12 158 1.44e-44 144

Find 38 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTTTAACACTAATGATTT+TGG 0.083726 5:+98205947 MsG0580029531.01.T01:three_prime_UTR
TCCAAAATCATTAGTGTTAA+AGG 0.147715 5:-98205948 None:intergenic
CATTATCAATTGCAACAATT+TGG 0.183397 5:-98205801 None:intergenic
CCAAAATCATTAGTGTTAAA+GGG 0.332799 5:-98205947 None:intergenic
GACTGGTAACTTAAAATTCA+AGG 0.371883 5:-98205494 None:intergenic
GCTCGAAGTGTTGGATTTGA+TGG 0.372933 5:+98203488 MsG0580029531.01.T01:CDS
TATAGAGCAGTGGAAGTATA+AGG 0.379763 5:+98205755 MsG0580029531.01.T01:CDS
TTTAAGTTACCAGTCTATAT+CGG 0.383846 5:+98205502 MsG0580029531.01.T01:CDS
ATTATTTAGTGTCCGCGATT+TGG 0.384448 5:+98205835 MsG0580029531.01.T01:three_prime_UTR
TAATCCTTTGCATACCGATT+CGG 0.416395 5:+98203460 MsG0580029531.01.T01:CDS
TCGGCTGCTGCTCGAAGTGT+TGG 0.445083 5:+98203479 MsG0580029531.01.T01:CDS
GGAGACATTCTTGTCGTTGA+AGG 0.449171 5:+98205701 MsG0580029531.01.T01:CDS
AAGACAAGATGCTTCAAGAA+TGG 0.472461 5:+98205680 MsG0580029531.01.T01:CDS
ATAATTAGAGAACATACTAA+TGG 0.476766 5:-98206062 None:intergenic
ATTAGAATCATGGCTCACTT+TGG 0.488635 5:-98203441 None:intergenic
ACTTCGAGCAGCAGCCGAAT+CGG 0.492615 5:-98203474 None:intergenic
GAGGTTTACCTTTAGTTTCG+TGG 0.497541 5:+98205996 MsG0580029531.01.T01:three_prime_UTR
TGTTGGATTTGATGGTCCAT+TGG 0.521699 5:+98203496 MsG0580029531.01.T01:CDS
TATGCAAAGGATTAGAATCA+TGG 0.523318 5:-98203451 None:intergenic
TGTCGTTGAAGGTGAAGCAT+TGG 0.530286 5:+98205712 MsG0580029531.01.T01:CDS
ATCTGATCTCCGATATAGAC+TGG 0.540031 5:-98205511 None:intergenic
TATATCGGAGATCAGATTGT+TGG 0.545806 5:+98205517 MsG0580029531.01.T01:CDS
CTTCCACTGCTCTATAGTCA+AGG 0.549215 5:-98205748 None:intergenic
ACAAATTGCCACGAAACTAA+AGG 0.552719 5:-98206004 None:intergenic
TGAGATACATACACAGCTGC+AGG 0.568759 5:-98205469 None:intergenic
CTATACAACTCTGAATCAGA+TGG 0.573952 5:+98205885 MsG0580029531.01.T01:three_prime_UTR
ACTCCTCATGTACTTAAACC+TGG 0.600357 5:+98203365 MsG0580029531.01.T01:CDS
CTGTACTGTAATAGTGAGTG+AGG 0.608224 5:+98205977 MsG0580029531.01.T01:three_prime_UTR
TTCCACTGCTCTATAGTCAA+GGG 0.621924 5:-98205747 None:intergenic
GTACTGAAGAACATCTTCCT+CGG 0.633973 5:-98203417 None:intergenic
ACACTAAATAATGAAACATG+AGG 0.638542 5:-98205824 None:intergenic
TACTAGAATGTAAACTCCTG+AGG 0.642498 5:-98203301 None:intergenic
AACTTCAGTATCTAAGTCTG+AGG 0.645534 5:+98203327 MsG0580029531.01.T01:CDS
ACTGCTCTATAGTCAAGGGT+TGG 0.652806 5:-98205743 None:intergenic
TCACCAGGTTTAAGTACATG+AGG 0.653739 5:-98203368 None:intergenic
AACCCTTGACTATAGAGCAG+TGG 0.656459 5:+98205745 MsG0580029531.01.T01:CDS
TGCAAAACATAACCAAATCG+CGG 0.659977 5:-98205847 None:intergenic
GCAGCCGAATCGGTATGCAA+AGG 0.665902 5:-98203464 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGATCAAATATAATATAT+AGG + Chr5:98203897-98203916 MsG0580029531.01.T01:intron 10.0%
!! AAGATCAAATATAATATATA+GGG + Chr5:98203898-98203917 MsG0580029531.01.T01:intron 10.0%
!! ATATAATAGATATGATATTA+TGG + Chr5:98205101-98205120 MsG0580029531.01.T01:intron 10.0%
!! ATTTAATACATGTAAATAAT+TGG + Chr5:98205314-98205333 MsG0580029531.01.T01:intron 10.0%
!! TTTAATACATGTAAATAATT+GGG + Chr5:98205315-98205334 MsG0580029531.01.T01:intron 10.0%
!!! TGAAAAAATTGATTATTTTT+TGG + Chr5:98203664-98203683 MsG0580029531.01.T01:intron 10.0%
!! AATTACACATATTATTATTC+AGG + Chr5:98203691-98203710 MsG0580029531.01.T01:intron 15.0%
!! TACATGTATTAAATAGAATA+AGG - Chr5:98205308-98205327 None:intergenic 15.0%
!!! AAGATGTTATAGAAATTTTA+AGG + Chr5:98204549-98204568 MsG0580029531.01.T01:intron 15.0%
!!! AATAAAGATTTTAGAAGAAT+GGG + Chr5:98203718-98203737 MsG0580029531.01.T01:intron 15.0%
!!! GTTTATTAGATATACATTTT+TGG + Chr5:98204590-98204609 MsG0580029531.01.T01:intron 15.0%
!! ATAATTAGAGAACATACTAA+TGG - Chr5:98206065-98206084 None:intergenic 20.0%
!! ATAATTCACAATGAGAAAAT+TGG - Chr5:98204771-98204790 None:intergenic 20.0%
!! ATCTTAATCTTTCTAATAAG+AGG - Chr5:98204340-98204359 None:intergenic 20.0%
!! ATTATATCCAACAATACTAT+GGG + Chr5:98205139-98205158 MsG0580029531.01.T01:intron 20.0%
!! TAATTCACAATGAGAAAATT+GGG - Chr5:98204770-98204789 None:intergenic 20.0%
!! TATTATATCCAACAATACTA+TGG + Chr5:98205138-98205157 MsG0580029531.01.T01:intron 20.0%
!! TGAAAATAACCACAAAATAT+TGG + Chr5:98204688-98204707 MsG0580029531.01.T01:intron 20.0%
!!! AAGATGTCATAGAAATTTTA+AGG + Chr5:98204355-98204374 MsG0580029531.01.T01:intron 20.0%
!!! AGTATCTATGATCAATTTTT+GGG + Chr5:98205915-98205934 MsG0580029531.01.T01:three_prime_UTR 20.0%
!!! CTTCACTTTTCTAATATTAT+GGG + Chr5:98205435-98205454 MsG0580029531.01.T01:intron 20.0%
!!! GAATAAAGATTTTAGAAGAA+TGG + Chr5:98203717-98203736 MsG0580029531.01.T01:intron 20.0%
!!! TCTTCACTTTTCTAATATTA+TGG + Chr5:98205434-98205453 MsG0580029531.01.T01:intron 20.0%
!!! TGATGTTTTGAAAAAAACAA+GGG + Chr5:98203388-98203407 MsG0580029531.01.T01:CDS 20.0%
! ACAAAATATTGGATTCTCAT+TGG + Chr5:98204699-98204718 MsG0580029531.01.T01:intron 25.0%
! ACAAGAATAGGAATGAAATT+TGG + Chr5:98203861-98203880 MsG0580029531.01.T01:intron 25.0%
! ACACTAAATAATGAAACATG+AGG - Chr5:98205827-98205846 None:intergenic 25.0%
! CATTATCAATTGCAACAATT+TGG - Chr5:98205804-98205823 None:intergenic 25.0%
! CCAAAATCATTAGTGTTAAA+GGG - Chr5:98205950-98205969 None:intergenic 25.0%
! GATGAACATTTAATGTCATT+CGG - Chr5:98205286-98205305 None:intergenic 25.0%
! TATAATATATAGGGTCTGTT+TGG + Chr5:98203907-98203926 MsG0580029531.01.T01:intron 25.0%
! TCCAAAATCATTAGTGTTAA+AGG - Chr5:98205951-98205970 None:intergenic 25.0%
! TGTAATTAACTGACAAGAAT+AGG + Chr5:98203849-98203868 MsG0580029531.01.T01:intron 25.0%
! TTGAGTAATAATGTGAATTG+AGG + Chr5:98205048-98205067 MsG0580029531.01.T01:intron 25.0%
! TTGTAAAACAGTTAGGAAAA+TGG + Chr5:98204074-98204093 MsG0580029531.01.T01:intron 25.0%
! TTGTCAGTTAATTACATTAG+AGG - Chr5:98203845-98203864 None:intergenic 25.0%
!! AATGAGAATCCAATATTTTG+TGG - Chr5:98204700-98204719 None:intergenic 25.0%
!! ATTAAGTTGTTTATCCAAAC+AGG + Chr5:98204045-98204064 MsG0580029531.01.T01:intron 25.0%
!! GAGTATCTATGATCAATTTT+TGG + Chr5:98205914-98205933 MsG0580029531.01.T01:three_prime_UTR 25.0%
!! GATTGACTATTTTCATAATG+CGG + Chr5:98204960-98204979 MsG0580029531.01.T01:intron 25.0%
!! TTAAGTTGTTTATCCAAACA+GGG + Chr5:98204046-98204065 MsG0580029531.01.T01:intron 25.0%
!! TTTAAGTTACCAGTCTATAT+CGG + Chr5:98205502-98205521 MsG0580029531.01.T01:CDS 25.0%
!!! GTGATGTTTTGAAAAAAACA+AGG + Chr5:98203387-98203406 MsG0580029531.01.T01:CDS 25.0%
!!! TCTTGTGCATTTTTTTGAAT+TGG + Chr5:98205604-98205623 MsG0580029531.01.T01:intron 25.0%
!!! TGACTGAAAGAAGTTTTAAA+GGG - Chr5:98204192-98204211 None:intergenic 25.0%
!!! TTGACTGAAAGAAGTTTTAA+AGG - Chr5:98204193-98204212 None:intergenic 25.0%
!!! TTTTCAGTGTTATTGAATAG+CGG + Chr5:98204806-98204825 MsG0580029531.01.T01:intron 25.0%
AAATGTGATGAGATGATATG+CGG - Chr5:98203551-98203570 None:intergenic 30.0%
AATGAAACAGATCCATTTCA+GGG + Chr5:98203746-98203765 MsG0580029531.01.T01:intron 30.0%
AATTACAAAGTACACTTACG+TGG - Chr5:98204662-98204681 None:intergenic 30.0%
AGTTATTCCCATAGTATTGT+TGG - Chr5:98205149-98205168 None:intergenic 30.0%
GACTGGTAACTTAAAATTCA+AGG - Chr5:98205497-98205516 None:intergenic 30.0%
TAGCATAAACACTTACCATA+TGG - Chr5:98204015-98204034 None:intergenic 30.0%
TATGCAAAGGATTAGAATCA+TGG - Chr5:98203454-98203473 None:intergenic 30.0%
TATTGAAGGATGAAGTGAAA+AGG + Chr5:98204254-98204273 MsG0580029531.01.T01:intron 30.0%
TGAATCTCAGAATCAAATCT+AGG - Chr5:98204159-98204178 None:intergenic 30.0%
TTCAAAAAAATGCACAAGAC+AGG - Chr5:98205603-98205622 None:intergenic 30.0%
! CCCTTTAACACTAATGATTT+TGG + Chr5:98205947-98205966 MsG0580029531.01.T01:three_prime_UTR 30.0%
! GTAATATCACGAGTTTTCAT+GGG - Chr5:98204640-98204659 None:intergenic 30.0%
!! AAACTGACGATAAGTATTGA+AGG + Chr5:98204240-98204259 MsG0580029531.01.T01:intron 30.0%
!! AATTTCTAGCAGAGATATCA+AGG - Chr5:98203611-98203630 None:intergenic 30.0%
!! TTGTACTAATCAGAGTATTG+CGG - Chr5:98204865-98204884 None:intergenic 30.0%
!! TTTAAAGAGAGCATTAGCAT+GGG - Chr5:98203581-98203600 None:intergenic 30.0%
!!! GTTTTTATGTTGCTTTGGTT+GGG - Chr5:98205261-98205280 None:intergenic 30.0%
!!! GTTTTTTTCAAAACATCACC+AGG - Chr5:98203386-98203405 None:intergenic 30.0%
!!! TGTTTTTATGTTGCTTTGGT+TGG - Chr5:98205262-98205281 None:intergenic 30.0%
!!! TTTTAAAGAGAGCATTAGCA+TGG - Chr5:98203582-98203601 None:intergenic 30.0%
AACTTCAGTATCTAAGTCTG+AGG + Chr5:98203327-98203346 MsG0580029531.01.T01:CDS 35.0%
AAGACAAGATGCTTCAAGAA+TGG + Chr5:98205680-98205699 MsG0580029531.01.T01:CDS 35.0%
ACAAATTGCCACGAAACTAA+AGG - Chr5:98206007-98206026 None:intergenic 35.0%
ACCTTCGTGTTATGTTAGTA+GGG - Chr5:98205354-98205373 None:intergenic 35.0%
AGACTAATAGACATTTGCTC+AGG - Chr5:98203772-98203791 None:intergenic 35.0%
ATTAGAAAAGTGAAGAAGCC+AGG - Chr5:98205430-98205449 None:intergenic 35.0%
ATTAGAATCATGGCTCACTT+TGG - Chr5:98203444-98203463 None:intergenic 35.0%
ATTATTTAGTGTCCGCGATT+TGG + Chr5:98205835-98205854 MsG0580029531.01.T01:three_prime_UTR 35.0%
CTATACAACTCTGAATCAGA+TGG + Chr5:98205885-98205904 MsG0580029531.01.T01:three_prime_UTR 35.0%
GAATGAAACAGATCCATTTC+AGG + Chr5:98203745-98203764 MsG0580029531.01.T01:intron 35.0%
GGCGATATTGTAAAACAGTT+AGG + Chr5:98204067-98204086 MsG0580029531.01.T01:intron 35.0%
TACTAGAATGTAAACTCCTG+AGG - Chr5:98203304-98203323 None:intergenic 35.0%
TAGCTTATGACATGTCCATA+TGG + Chr5:98203997-98204016 MsG0580029531.01.T01:intron 35.0%
TAGTGACTTACACAACTTAG+AGG + Chr5:98205215-98205234 MsG0580029531.01.T01:intron 35.0%
TATATCGGAGATCAGATTGT+TGG + Chr5:98205517-98205536 MsG0580029531.01.T01:CDS 35.0%
TGCAAAACATAACCAAATCG+CGG - Chr5:98205850-98205869 None:intergenic 35.0%
TTATTGAATAGCGGCTATAG+TGG + Chr5:98204815-98204834 MsG0580029531.01.T01:intron 35.0%
! AAAAACAAGGGTTTTTACCG+AGG + Chr5:98203400-98203419 MsG0580029531.01.T01:CDS 35.0%
! AATAATTGGGCCTTGTCTAT+TGG + Chr5:98205328-98205347 MsG0580029531.01.T01:intron 35.0%
! ACTGTATTTTCTCTCTGTTC+TGG - Chr5:98205189-98205208 None:intergenic 35.0%
! AGTTTCTTCGTTTTCTTTCC+TGG + Chr5:98205409-98205428 MsG0580029531.01.T01:intron 35.0%
! GGTAATATCACGAGTTTTCA+TGG - Chr5:98204641-98204660 None:intergenic 35.0%
! TAATCCTTTGCATACCGATT+CGG + Chr5:98203460-98203479 MsG0580029531.01.T01:CDS 35.0%
! TATAGAGCAGTGGAAGTATA+AGG + Chr5:98205755-98205774 MsG0580029531.01.T01:CDS 35.0%
! TTTTACAATATCGCCCTGTT+TGG - Chr5:98204062-98204081 None:intergenic 35.0%
!! TCATGACAGATGTTAGCTTT+AGG + Chr5:98204130-98204149 MsG0580029531.01.T01:intron 35.0%
!!! AAAGAAGTTTTAAAGGGCAG+CGG - Chr5:98204186-98204205 None:intergenic 35.0%
!!! CGGTTGTTTTTATGTTGCTT+TGG - Chr5:98205266-98205285 None:intergenic 35.0%
AAGCACTTGAGTAGAAGTCT+TGG - Chr5:98205030-98205049 None:intergenic 40.0%
ACAAGCATTCCATGTACCAA+TGG - Chr5:98203515-98203534 None:intergenic 40.0%
ACTCCTCATGTACTTAAACC+TGG + Chr5:98203365-98203384 MsG0580029531.01.T01:CDS 40.0%
ATCTGATCTCCGATATAGAC+TGG - Chr5:98205514-98205533 None:intergenic 40.0%
CTGTACTGTAATAGTGAGTG+AGG + Chr5:98205977-98205996 MsG0580029531.01.T01:three_prime_UTR 40.0%
GACCTTCGTGTTATGTTAGT+AGG - Chr5:98205355-98205374 None:intergenic 40.0%
GTACTGAAGAACATCTTCCT+CGG - Chr5:98203420-98203439 None:intergenic 40.0%
TAATAGCAGCCATAGTACTG+TGG + Chr5:98204894-98204913 MsG0580029531.01.T01:intron 40.0%
TCACCAGGTTTAAGTACATG+AGG - Chr5:98203371-98203390 None:intergenic 40.0%
TCATGAAAGAACTAGAGCGA+AGG - Chr5:98205377-98205396 None:intergenic 40.0%
TTCCACTGCTCTATAGTCAA+GGG - Chr5:98205750-98205769 None:intergenic 40.0%
! GAGGTTTACCTTTAGTTTCG+TGG + Chr5:98205996-98206015 MsG0580029531.01.T01:three_prime_UTR 40.0%
! TGTTGGATTTGATGGTCCAT+TGG + Chr5:98203496-98203515 MsG0580029531.01.T01:CDS 40.0%
! TTTGATGGTCCATTGGTACA+TGG + Chr5:98203503-98203522 MsG0580029531.01.T01:intron 40.0%
!!! AAGAAGTTTTAAAGGGCAGC+GGG - Chr5:98204185-98204204 None:intergenic 40.0%
AACCCTTGACTATAGAGCAG+TGG + Chr5:98205745-98205764 MsG0580029531.01.T01:CDS 45.0%
ACTGCTCTATAGTCAAGGGT+TGG - Chr5:98205746-98205765 None:intergenic 45.0%
CTTCCACTGCTCTATAGTCA+AGG - Chr5:98205751-98205770 None:intergenic 45.0%
GCAGATCGCATCAAATAGAG+AGG - Chr5:98204940-98204959 None:intergenic 45.0%
GCCCTACTAACATAACACGA+AGG + Chr5:98205350-98205369 MsG0580029531.01.T01:intron 45.0%
GCGGTGAAAATATGCATGCA+TGG + Chr5:98204979-98204998 MsG0580029531.01.T01:intron 45.0%
GGAATAACTCTCTGCAGAGT+TGG + Chr5:98205160-98205179 MsG0580029531.01.T01:intron 45.0%
GGAGACATTCTTGTCGTTGA+AGG + Chr5:98205701-98205720 MsG0580029531.01.T01:CDS 45.0%
GTTAGTAGGGCCAATAGACA+AGG - Chr5:98205341-98205360 None:intergenic 45.0%
TCTGCTATGCCACAGTACTA+TGG - Chr5:98204906-98204925 None:intergenic 45.0%
TGAGATACATACACAGCTGC+AGG - Chr5:98205472-98205491 None:intergenic 45.0%
TGTCGTTGAAGGTGAAGCAT+TGG + Chr5:98205712-98205731 MsG0580029531.01.T01:CDS 45.0%
! CACGAGTTTTCATGGGATGT+TGG - Chr5:98204633-98204652 None:intergenic 45.0%
!! GCTCGAAGTGTTGGATTTGA+TGG + Chr5:98203488-98203507 MsG0580029531.01.T01:CDS 45.0%
CATTTGCTCAGGCCCTGAAA+TGG - Chr5:98203761-98203780 None:intergenic 50.0%
ACTTCGAGCAGCAGCCGAAT+CGG - Chr5:98203477-98203496 None:intergenic 55.0%
GCAGCCGAATCGGTATGCAA+AGG - Chr5:98203467-98203486 None:intergenic 55.0%
TCGGCTGCTGCTCGAAGTGT+TGG + Chr5:98203479-98203498 MsG0580029531.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 98203296 98206117 98203296 ID=MsG0580029531.01;Name=MsG0580029531.01
Chr5 mRNA 98203296 98206117 98203296 ID=MsG0580029531.01.T01;Parent=MsG0580029531.01;Name=MsG0580029531.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0.5|0.66|1|1|1|3|342|147
Chr5 exon 98203296 98203517 98203296 ID=MsG0580029531.01.T01:exon:25556;Parent=MsG0580029531.01.T01
Chr5 exon 98205469 98205584 98205469 ID=MsG0580029531.01.T01:exon:25557;Parent=MsG0580029531.01.T01
Chr5 exon 98205670 98206117 98205670 ID=MsG0580029531.01.T01:exon:25558;Parent=MsG0580029531.01.T01
Chr5 CDS 98203296 98203517 98203296 ID=MsG0580029531.01.T01:cds;Parent=MsG0580029531.01.T01
Chr5 CDS 98205469 98205584 98205469 ID=MsG0580029531.01.T01:cds;Parent=MsG0580029531.01.T01
Chr5 CDS 98205670 98205775 98205670 ID=MsG0580029531.01.T01:cds;Parent=MsG0580029531.01.T01
Chr5 three_prime_UTR 98205776 98206117 98205776 ID=MsG0580029531.01.T01:three_prime_utr;Parent=MsG0580029531.01.T01
Gene Sequence

>MsG0580029531.01.T01

ATGCTCCTCAGGAGTTTACATTCTAGTAGCAAACTTCAGTATCTAAGTCTGAGGTGTTTTTCATCTACAACTCCTCATGTACTTAAACCTGGTGATGTTTTGAAAAAAACAAGGGTTTTTACCGAGGAAGATGTTCTTCAGTACTCCAAAGTGAGCCATGATTCTAATCCTTTGCATACCGATTCGGCTGCTGCTCGAAGTGTTGGATTTGATGGTCCATTGCCTGCAGCTGTGTATGTATCTCAAACCTTGAATTTTAAGTTACCAGTCTATATCGGAGATCAGATTGTTGGTGAAGTACAAGCAACTAATCTACGAGAAAACAAAAATCGATATTTTGCGAAGTTTAAGACAAGATGCTTCAAGAATGGAGACATTCTTGTCGTTGAAGGTGAAGCATTGGCTTTGTTACCAACCCTTGACTATAGAGCAGTGGAAGTATAA
Protein sequence

>MsG0580029531.01.T01

MLLRSLHSSSKLQYLSLRCFSSTTPHVLKPGDVLKKTRVFTEEDVLQYSKVSHDSNPLHTDSAAARSVGFDGPLPAAVYVSQTLNFKLPVYIGDQIVGEVQATNLRENKNRYFAKFKTRCFKNGDILVVEGEALALLPTLDYRAVEV*