AlfalfaGEDB | MsG0580029954.01

M. sativa cultivar ZhongmuNo.1 / MsG0580029954.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029954.01.T01	AET00753.1	76.884	199	34	4	1	189	75	271	6.59E-61	201

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029954.01.T01	G7K4N7	76.884	199	34	4	1	189	75	271	3.15e-61	201

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0580025240.01	MsG0580029954.01	0.802262	6.097001e-49	2.973286e-46
MsG0380015754.01	MsG0580029954.01	0.813422	2.580896e-51	1.684714e-48
MsG0580029414.01	MsG0580029954.01	0.816023	6.846754e-52	4.796872e-49
MsG0580029954.01	MsG0780039975.01	0.812205	4.765364e-51	3.011003e-48
MsG0580029954.01	MsG0780041004.01	0.802896	4.513790e-49	2.237049e-46
MsG0580029954.01	MsG0880044538.01	0.806416	8.314379e-50	4.510181e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580029954.01.T01	MTR_5g095540	76.884	199	34	4	1	189	75	271	7.98e-65	201
MsG0580029954.01.T01	MTR_5g095630	60.317	189	62	2	1	189	75	250	4.11e-41	139

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCTACTTCTTTAGAAAA+TGG	0.214081	5:-104436526	None:intergenic
TGATGAATCTTCCGAGTTAT+TGG	0.252316	5:-104436344	None:intergenic
TCTCTCCAACATTGGCTCAT+TGG	0.315687	5:-104436434	None:intergenic
GCTTCAACATTCTGTTCATC+AGG	0.375456	5:-104436481	None:intergenic
AGCATGTTCTTCATTTGTAA+TGG	0.413583	5:+104436256	None:intergenic
TCAGCTTCTCTCTCCAACAT+TGG	0.445320	5:-104436442	None:intergenic
TGCTAGACGGCATTTAATCA+TGG	0.458364	5:+104436801	MsG0580029954.01.T01:CDS
ATATCATGTTCTTGATGTTG+AGG	0.561116	5:+104436411	MsG0580029954.01.T01:CDS
CGAAGAAAACAACATAGAAG+AGG	0.563412	5:+104436666	MsG0580029954.01.T01:CDS
GAATCTTCCGAGTTATTGGA+AGG	0.566810	5:-104436340	None:intergenic
TTTATCATCATCACTAGAAG+AGG	0.567340	5:-104436317	None:intergenic
TGACAAGGGAGATAAATTGT+CGG	0.590740	5:+104436624	MsG0580029954.01.T01:CDS
ATTTAAGAATTGATGCTAGA+CGG	0.591072	5:+104436788	MsG0580029954.01.T01:CDS
TTTATAGAGCAAGAAGAACA+AGG	0.591980	5:+104436568	MsG0580029954.01.T01:CDS
TGATAAACCTTCCAATAACT+CGG	0.596540	5:+104436333	MsG0580029954.01.T01:CDS
TGAACAGAATGTTGAAGCAG+AGG	0.598499	5:+104436486	MsG0580029954.01.T01:CDS
TAGTTAAACCAACGAAAACA+AGG	0.611579	5:-104436282	None:intergenic
TGAGGCCAATGAGCCAATGT+TGG	0.620191	5:+104436429	MsG0580029954.01.T01:CDS
ATTTGATGCACATGATGACA+AGG	0.638307	5:+104436609	MsG0580029954.01.T01:CDS
TTTGATGCACATGATGACAA+GGG	0.703672	5:+104436610	MsG0580029954.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATGAAATCTTCAAATTTT+TGG	-	Chr5:104436753-104436772	None:intergenic	15.0%
!	ATTTAAGAATTGATGCTAGA+CGG	+	Chr5:104436788-104436807	MsG0580029954.01.T01:CDS	25.0%
!!	TCTTCTACTTCTTTAGAAAA+TGG	-	Chr5:104436529-104436548	None:intergenic	25.0%
	ATATCATGTTCTTGATGTTG+AGG	+	Chr5:104436411-104436430	MsG0580029954.01.T01:CDS	30.0%
	TAGTTAAACCAACGAAAACA+AGG	-	Chr5:104436285-104436304	None:intergenic	30.0%
	TGATAAACCTTCCAATAACT+CGG	+	Chr5:104436333-104436352	MsG0580029954.01.T01:CDS	30.0%
	TTTATAGAGCAAGAAGAACA+AGG	+	Chr5:104436568-104436587	MsG0580029954.01.T01:CDS	30.0%
	TTTATCATCATCACTAGAAG+AGG	-	Chr5:104436320-104436339	None:intergenic	30.0%
	ATTTGATGCACATGATGACA+AGG	+	Chr5:104436609-104436628	MsG0580029954.01.T01:CDS	35.0%
	CGAAGAAAACAACATAGAAG+AGG	+	Chr5:104436666-104436685	MsG0580029954.01.T01:CDS	35.0%
	TGACAAGGGAGATAAATTGT+CGG	+	Chr5:104436624-104436643	MsG0580029954.01.T01:CDS	35.0%
	TGATGAATCTTCCGAGTTAT+TGG	-	Chr5:104436347-104436366	None:intergenic	35.0%
	TTTGATGCACATGATGACAA+GGG	+	Chr5:104436610-104436629	MsG0580029954.01.T01:CDS	35.0%
	GAATCTTCCGAGTTATTGGA+AGG	-	Chr5:104436343-104436362	None:intergenic	40.0%
	GCTTCAACATTCTGTTCATC+AGG	-	Chr5:104436484-104436503	None:intergenic	40.0%
	TGAACAGAATGTTGAAGCAG+AGG	+	Chr5:104436486-104436505	MsG0580029954.01.T01:CDS	40.0%
	TGCTAGACGGCATTTAATCA+TGG	+	Chr5:104436801-104436820	MsG0580029954.01.T01:CDS	40.0%
!!!	AATGGTCTCCTTGTTTTCGT+TGG	+	Chr5:104436274-104436293	MsG0580029954.01.T01:CDS	40.0%
	TCAGCTTCTCTCTCCAACAT+TGG	-	Chr5:104436445-104436464	None:intergenic	45.0%
!!	TCTCTCCAACATTGGCTCAT+TGG	-	Chr5:104436437-104436456	None:intergenic	45.0%
	TGAGGCCAATGAGCCAATGT+TGG	+	Chr5:104436429-104436448	MsG0580029954.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	104436259	104436828	104436259	ID=MsG0580029954.01;Name=MsG0580029954.01
Chr5	mRNA	104436259	104436828	104436259	ID=MsG0580029954.01.T01;Parent=MsG0580029954.01;Name=MsG0580029954.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|189
Chr5	exon	104436259	104436828	104436259	ID=MsG0580029954.01.T01:exon:7127;Parent=MsG0580029954.01.T01
Chr5	CDS	104436259	104436828	104436259	ID=MsG0580029954.01.T01:cds;Parent=MsG0580029954.01.T01

Gene Sequence

>MsG0580029954.01.T01

ATGTTCTTCATTTGTAATGGTCTCCTTGTTTTCGTTGGTTTAACTAAATCTTTCTCTGCCTCTTCTAGTGATGATGATAAACCTTCCAATAACTCGGAAGATTCATCAACATCACAGTATATTCAAGAAGAAGTATACGAAGATGTTTCACGATATCATGTTCTTGATGTTGAGGCCAATGAGCCAATGTTGGAGAGAGAAGCTGAGAAGATGACTAGTGAGCCTGATGAACAGAATGTTGAAGCAGAGGAAGAAATAGTAATAAAACCATTTTCTAAAGAAGTAGAAGAAAATATTGAAAATATGATTTTTATAGAGCAAGAAGAACAAGGAGAAGAAGAAATTGAACTATTTGATGCACATGATGACAAGGGAGATAAATTGTCGGAAATTGATTACATTCTAATCGAAGAAAACAACATAGAAGAGGAAGAAGAAGAAGATTATGTTGAAGAAGAAGAAAGTAGTGTGTTAAGTACAGAAGAGTTAAACCAAAAATTTGAAGATTTCATTAGAAAGATGAAAGAAGATTTAAGAATTGATGCTAGACGGCATTTAATCATGGTTTAA

Protein sequence

>MsG0580029954.01.T01

MFFICNGLLVFVGLTKSFSASSSDDDKPSNNSEDSSTSQYIQEEVYEDVSRYHVLDVEANEPMLEREAEKMTSEPDEQNVEAEEEIVIKPFSKEVEENIENMIFIEQEEQGEEEIELFDAHDDKGDKLSEIDYILIEENNIEEEEEEDYVEEEESSVLSTEELNQKFEDFIRKMKEDLRIDARRHLIMV*