Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025240.01.T01 | XP_003612452.1 | 100 | 114 | 0 | 0 | 1 | 114 | 1 | 114 | 1.83E-78 | 237 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025240.01.T01 | Q9C9A3 | 60 | 120 | 43 | 2 | 1 | 115 | 1 | 120 | 2.31E-43 | 142 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025240.01.T01 | G7K2P7 | 100.000 | 114 | 0 | 0 | 1 | 114 | 1 | 114 | 8.74e-79 | 237 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048845.01 | MsG0580025240.01 | 0.815050 | 1.127473e-51 | 7.690990e-49 |
| MsG0080049130.01 | MsG0580025240.01 | 0.811705 | 6.125421e-51 | 3.818401e-48 |
| MsG0180000843.01 | MsG0580025240.01 | 0.823747 | 1.172415e-53 | 1.018493e-50 |
| MsG0180001100.01 | MsG0580025240.01 | 0.806309 | 8.755236e-50 | 4.736548e-47 |
| MsG0180001685.01 | MsG0580025240.01 | 0.804176 | 2.449642e-49 | 1.254545e-46 |
| MsG0180003316.01 | MsG0580025240.01 | 0.804375 | 2.226547e-49 | 1.146179e-46 |
| MsG0180004934.01 | MsG0580025240.01 | 0.809964 | 1.458497e-50 | 8.683380e-48 |
| MsG0180005829.01 | MsG0580025240.01 | 0.800934 | 1.141787e-48 | 5.383968e-46 |
| MsG0280010975.01 | MsG0580025240.01 | 0.803259 | 3.795955e-49 | 1.898796e-46 |
| MsG0280011270.01 | MsG0580025240.01 | 0.834509 | 2.876818e-56 | 3.421567e-53 |
| MsG0380013177.01 | MsG0580025240.01 | 0.822803 | 1.947923e-53 | 1.647417e-50 |
| MsG0480021367.01 | MsG0580025240.01 | 0.811422 | 7.056108e-51 | 4.366039e-48 |
| MsG0480021406.01 | MsG0580025240.01 | 0.802935 | 4.429857e-49 | 2.197554e-46 |
| MsG0480022617.01 | MsG0580025240.01 | 0.805952 | 1.041270e-49 | 5.582201e-47 |
| MsG0480022664.01 | MsG0580025240.01 | 0.803317 | 3.692440e-49 | 1.849739e-46 |
| MsG0480022805.01 | MsG0580025240.01 | 0.800466 | 1.422303e-48 | 6.627117e-46 |
| MsG0480022918.01 | MsG0580025240.01 | 0.802609 | 5.172053e-49 | 2.544577e-46 |
| MsG0480023578.01 | MsG0580025240.01 | 0.853200 | 2.796302e-61 | 5.993981e-58 |
| MsG0580024717.01 | MsG0580025240.01 | 0.821108 | 4.810172e-53 | 3.877773e-50 |
| MsG0580024836.01 | MsG0580025240.01 | 0.809600 | 1.746821e-50 | 1.030089e-47 |
| MsG0580024976.01 | MsG0580025240.01 | -0.808857 | 2.520431e-50 | 1.456978e-47 |
| MsG0580025240.01 | MsG0580027331.01 | 0.810430 | 1.157065e-50 | 6.972995e-48 |
| MsG0580025240.01 | MsG0580027899.01 | 0.804366 | 2.236027e-49 | 1.150782e-46 |
| MsG0580025240.01 | MsG0580028024.01 | 0.803128 | 4.041078e-49 | 2.014583e-46 |
| MsG0580025240.01 | MsG0580028925.01 | 0.840477 | 8.495419e-58 | 1.211501e-54 |
| MsG0580025240.01 | MsG0580029276.01 | 0.802603 | 5.187929e-49 | 2.551988e-46 |
| MsG0580025240.01 | MsG0580029389.01 | 0.815622 | 8.410646e-52 | 5.828697e-49 |
| MsG0580025240.01 | MsG0580029954.01 | 0.802262 | 6.097001e-49 | 2.973286e-46 |
| MsG0580025240.01 | MsG0580029967.01 | 0.808344 | 3.244049e-50 | 1.850360e-47 |
| MsG0580025240.01 | MsG0680030464.01 | 0.806496 | 7.996099e-50 | 4.346507e-47 |
| MsG0580025240.01 | MsG0680030680.01 | 0.801773 | 7.687546e-49 | 3.702393e-46 |
| MsG0580025240.01 | MsG0680032000.01 | 0.810161 | 1.322568e-50 | 7.914417e-48 |
| MsG0580025240.01 | MsG0680032003.01 | 0.804477 | 2.120497e-49 | 1.094394e-46 |
| MsG0580025240.01 | MsG0680035308.01 | 0.814474 | 1.512634e-51 | 1.016002e-48 |
| MsG0580025240.01 | MsG0680035664.01 | 0.808025 | 3.792266e-50 | 2.144881e-47 |
| MsG0580025240.01 | MsG0780037946.01 | 0.838706 | 2.452723e-57 | 3.312392e-54 |
| MsG0580025240.01 | MsG0780039233.01 | 0.805484 | 1.305463e-49 | 6.914813e-47 |
| MsG0580025240.01 | MsG0780040080.01 | 0.806853 | 6.722044e-50 | 3.687708e-47 |
| MsG0580025240.01 | MsG0780040319.01 | 0.809767 | 1.607655e-50 | 9.522452e-48 |
| MsG0580025240.01 | MsG0780041004.01 | 0.834278 | 3.287904e-56 | 3.883326e-53 |
| MsG0580025240.01 | MsG0780041778.01 | 0.825171 | 5.422391e-54 | 4.905283e-51 |
| MsG0580025240.01 | MsG0880042343.01 | 0.817042 | 4.049019e-52 | 2.916930e-49 |
| MsG0580025240.01 | MsG0880042690.01 | 0.831560 | 1.557782e-55 | 1.696712e-52 |
| MsG0580025240.01 | MsG0880042876.01 | 0.801413 | 9.112212e-49 | 4.348828e-46 |
| MsG0580025240.01 | MsG0880043235.01 | 0.834333 | 3.184309e-56 | 3.767247e-53 |
| MsG0580025240.01 | MsG0880043250.01 | 0.803645 | 3.157767e-49 | 1.595303e-46 |
| MsG0580025240.01 | MsG0880044105.01 | 0.823713 | 1.194132e-53 | 1.036383e-50 |
| MsG0580025240.01 | MsG0880044558.01 | 0.818969 | 1.484925e-52 | 1.128068e-49 |
| MsG0580025240.01 | MsG0880044884.01 | 0.820405 | 6.980443e-53 | 5.518411e-50 |
| MsG0580025240.01 | MsG0880046735.01 | 0.800745 | 1.247931e-48 | 5.855985e-46 |
| MsG0580025240.01 | MsG0880047156.01 | 0.804427 | 2.172011e-49 | 1.119525e-46 |
| MsG0580025240.01 | MsG0880047460.01 | -0.807400 | 5.148396e-50 | 2.864854e-47 |
| MsG0380014936.01 | MsG0580025240.01 | 0.802796 | 4.732095e-49 | 2.339367e-46 |
| MsG0380016452.01 | MsG0580025240.01 | 0.800290 | 1.545001e-48 | 7.167094e-46 |
| MsG0380016762.01 | MsG0580025240.01 | 0.818617 | 1.785084e-52 | 1.342748e-49 |
| MsG0380017033.01 | MsG0580025240.01 | 0.806934 | 6.463092e-50 | 3.552979e-47 |
| MsG0480018232.01 | MsG0580025240.01 | 0.801730 | 7.842849e-49 | 3.773125e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025240.01.T01 | MTR_5g025150 | 100.000 | 114 | 0 | 0 | 1 | 114 | 1 | 114 | 2.22e-82 | 237 |
| MsG0580025240.01.T01 | MTR_1g030630 | 77.586 | 116 | 24 | 1 | 1 | 114 | 1 | 116 | 2.24e-62 | 187 |
| MsG0580025240.01.T01 | MTR_6g003990 | 62.295 | 122 | 38 | 2 | 1 | 114 | 1 | 122 | 3.61e-49 | 154 |
| MsG0580025240.01.T01 | MTR_7g083060 | 63.793 | 116 | 40 | 2 | 1 | 114 | 1 | 116 | 8.45e-47 | 147 |
| MsG0580025240.01.T01 | MTR_3g087770 | 53.390 | 118 | 51 | 1 | 2 | 115 | 5 | 122 | 3.20e-39 | 129 |
| MsG0580025240.01.T01 | MTR_4g073040 | 56.667 | 120 | 46 | 2 | 1 | 114 | 1 | 120 | 4.33e-39 | 128 |
| MsG0580025240.01.T01 | MTR_8g091420 | 55.556 | 117 | 49 | 1 | 2 | 115 | 5 | 121 | 3.80e-38 | 126 |
| MsG0580025240.01.T01 | MTR_3g117890 | 51.695 | 118 | 53 | 2 | 1 | 114 | 1 | 118 | 7.80e-38 | 125 |
| MsG0580025240.01.T01 | MTR_6g003990 | 68.235 | 85 | 26 | 1 | 31 | 114 | 1 | 85 | 1.67e-37 | 123 |
| MsG0580025240.01.T01 | MTR_5g022620 | 52.893 | 121 | 50 | 2 | 1 | 114 | 1 | 121 | 1.06e-35 | 120 |
| MsG0580025240.01.T01 | MTR_5g055020 | 48.276 | 116 | 58 | 1 | 1 | 114 | 1 | 116 | 4.05e-35 | 118 |
| MsG0580025240.01.T01 | MTR_3g073670 | 49.123 | 114 | 58 | 0 | 2 | 115 | 5 | 118 | 3.76e-31 | 108 |
| MsG0580025240.01.T01 | MTR_1g078090 | 49.438 | 89 | 41 | 1 | 15 | 103 | 45 | 129 | 1.62e-23 | 89.7 |
| MsG0580025240.01.T01 | MTR_6g026890 | 45.556 | 90 | 46 | 2 | 15 | 102 | 6 | 94 | 5.98e-23 | 87.4 |
| MsG0580025240.01.T01 | MTR_3g010130 | 69.231 | 52 | 15 | 1 | 1 | 51 | 1 | 52 | 1.74e-18 | 73.6 |
| MsG0580025240.01.T01 | MTR_2g091235 | 43.939 | 66 | 37 | 0 | 22 | 87 | 1 | 66 | 1.69e-16 | 70.5 |
| MsG0580025240.01.T01 | MTR_1g082650 | 39.189 | 74 | 45 | 0 | 22 | 95 | 9 | 82 | 5.78e-14 | 67.0 |
| MsG0580025240.01.T01 | MTR_2g022250 | 42.623 | 61 | 35 | 0 | 27 | 87 | 14 | 74 | 3.31e-13 | 64.7 |
| MsG0580025240.01.T01 | MTR_4g117220 | 42.623 | 61 | 35 | 0 | 27 | 87 | 14 | 74 | 3.78e-13 | 64.7 |
| MsG0580025240.01.T01 | MTR_7g013660 | 38.462 | 91 | 54 | 1 | 23 | 113 | 4 | 92 | 6.36e-13 | 60.8 |
| MsG0580025240.01.T01 | MTR_7g108560 | 41.538 | 65 | 38 | 0 | 23 | 87 | 22 | 86 | 1.27e-12 | 62.8 |
| MsG0580025240.01.T01 | MTR_4g117140 | 38.889 | 72 | 44 | 0 | 16 | 87 | 3 | 74 | 1.69e-12 | 62.8 |
| MsG0580025240.01.T01 | MTR_1g107565 | 39.394 | 66 | 40 | 0 | 22 | 87 | 12 | 77 | 1.85e-12 | 62.4 |
| MsG0580025240.01.T01 | MTR_4g094238 | 36.471 | 85 | 51 | 2 | 22 | 106 | 1 | 82 | 3.81e-12 | 58.2 |
| MsG0580025240.01.T01 | MTR_1g092670 | 40.000 | 65 | 39 | 0 | 23 | 87 | 4 | 68 | 1.10e-11 | 56.6 |
| MsG0580025240.01.T01 | MTR_2g436830 | 39.474 | 76 | 44 | 1 | 15 | 88 | 5 | 80 | 8.58e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025240.01.T01 | AT1G71050 | 60.000 | 120 | 43 | 2 | 1 | 115 | 1 | 120 | 2.36e-44 | 142 |
| MsG0580025240.01.T01 | AT1G22990 | 56.557 | 122 | 46 | 2 | 1 | 115 | 1 | 122 | 2.12e-43 | 139 |
| MsG0580025240.01.T01 | AT5G17450 | 58.120 | 117 | 46 | 1 | 1 | 114 | 1 | 117 | 9.08e-42 | 135 |
| MsG0580025240.01.T01 | AT1G22990 | 53.279 | 122 | 45 | 3 | 1 | 115 | 1 | 117 | 1.89e-38 | 126 |
| MsG0580025240.01.T01 | AT4G38580 | 52.101 | 119 | 53 | 2 | 1 | 115 | 1 | 119 | 4.47e-35 | 118 |
| MsG0580025240.01.T01 | AT4G38580 | 52.101 | 119 | 53 | 2 | 1 | 115 | 1 | 119 | 4.47e-35 | 118 |
| MsG0580025240.01.T01 | AT4G39700 | 51.667 | 120 | 52 | 1 | 2 | 115 | 5 | 124 | 2.03e-34 | 117 |
| MsG0580025240.01.T01 | AT4G35060 | 44.444 | 117 | 62 | 2 | 1 | 114 | 1 | 117 | 4.49e-33 | 113 |
| MsG0580025240.01.T01 | AT5G66110 | 56.566 | 99 | 42 | 1 | 17 | 114 | 13 | 111 | 2.78e-31 | 108 |
| MsG0580025240.01.T01 | AT5G66110 | 56.566 | 99 | 42 | 1 | 17 | 114 | 13 | 111 | 2.78e-31 | 108 |
| MsG0580025240.01.T01 | AT4G08570 | 45.217 | 115 | 62 | 1 | 2 | 115 | 5 | 119 | 1.32e-29 | 104 |
| MsG0580025240.01.T01 | AT5G66110 | 54.118 | 85 | 38 | 1 | 31 | 114 | 1 | 85 | 4.52e-26 | 94.4 |
| MsG0580025240.01.T01 | AT5G17450 | 55.224 | 67 | 30 | 0 | 48 | 114 | 7 | 73 | 9.18e-24 | 88.2 |
| MsG0580025240.01.T01 | AT4G10465 | 49.474 | 95 | 45 | 2 | 10 | 104 | 39 | 130 | 4.80e-22 | 85.9 |
| MsG0580025240.01.T01 | AT2G18196 | 45.977 | 87 | 46 | 1 | 3 | 89 | 27 | 112 | 5.91e-22 | 85.5 |
| MsG0580025240.01.T01 | AT1G06330 | 54.286 | 70 | 30 | 1 | 22 | 89 | 1 | 70 | 1.52e-20 | 81.3 |
| MsG0580025240.01.T01 | AT3G56891 | 47.059 | 68 | 36 | 0 | 22 | 89 | 15 | 82 | 1.57e-19 | 79.0 |
| MsG0580025240.01.T01 | AT3G56891 | 49.153 | 59 | 30 | 0 | 31 | 89 | 1 | 59 | 2.01e-17 | 73.2 |
| MsG0580025240.01.T01 | AT2G18196 | 54.237 | 59 | 27 | 0 | 31 | 89 | 1 | 59 | 1.01e-16 | 70.9 |
| MsG0580025240.01.T01 | AT1G29100 | 37.349 | 83 | 45 | 1 | 25 | 107 | 5 | 80 | 7.72e-13 | 61.2 |
| MsG0580025240.01.T01 | AT1G66240 | 43.077 | 65 | 37 | 0 | 23 | 87 | 33 | 97 | 2.42e-12 | 58.9 |
| MsG0580025240.01.T01 | AT3G06130 | 37.879 | 66 | 41 | 0 | 22 | 87 | 9 | 74 | 5.49e-12 | 61.2 |
| MsG0580025240.01.T01 | AT3G06130 | 36.111 | 72 | 46 | 0 | 22 | 93 | 9 | 80 | 7.66e-12 | 60.8 |
| MsG0580025240.01.T01 | AT1G29100 | 35.802 | 81 | 45 | 1 | 27 | 107 | 1 | 74 | 1.41e-11 | 57.8 |
| MsG0580025240.01.T01 | AT5G19090 | 37.879 | 66 | 41 | 0 | 22 | 87 | 9 | 74 | 1.53e-11 | 60.1 |
| MsG0580025240.01.T01 | AT5G19090 | 37.879 | 66 | 41 | 0 | 22 | 87 | 9 | 74 | 1.53e-11 | 60.1 |
| MsG0580025240.01.T01 | AT5G19090 | 37.879 | 66 | 41 | 0 | 22 | 87 | 9 | 74 | 1.53e-11 | 60.1 |
| MsG0580025240.01.T01 | AT3G56240 | 40.909 | 66 | 39 | 0 | 23 | 88 | 3 | 68 | 1.76e-11 | 57.0 |
| MsG0580025240.01.T01 | AT3G56240 | 40.909 | 66 | 39 | 0 | 23 | 88 | 3 | 68 | 1.76e-11 | 57.0 |
| MsG0580025240.01.T01 | AT5G19090 | 37.879 | 66 | 41 | 0 | 22 | 87 | 9 | 74 | 1.86e-11 | 59.7 |
| MsG0580025240.01.T01 | AT5G19090 | 37.879 | 66 | 41 | 0 | 22 | 87 | 9 | 74 | 1.86e-11 | 59.7 |
Find 45 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAGACTTTGAAGCTATT+TGG | 0.178715 | 5:-15764819 | MsG0580025240.01.T01:three_prime_UTR |
| CCCTTATAAAAGTATATAAT+TGG | 0.222278 | 5:-15766301 | MsG0580025240.01.T01:five_prime_UTR |
| ATCTTATCATCTGTTCCATT+TGG | 0.247141 | 5:+15764959 | None:intergenic |
| AGTGGAAATAAAGGTGAAAA+TGG | 0.297522 | 5:-15765981 | MsG0580025240.01.T01:CDS |
| CTGTGCTCTGCACCTTCCTT+AGG | 0.323100 | 5:+15765108 | None:intergenic |
| TCTTATCATCTGTTCCATTT+GGG | 0.328722 | 5:+15764960 | None:intergenic |
| CACATGATTGAACATGCATT+AGG | 0.335274 | 5:+15764911 | None:intergenic |
| CTTATAGACAGTGGAAATAA+AGG | 0.347942 | 5:-15765990 | MsG0580025240.01.T01:intron |
| TCATCTGTTCCATTTGGGTT+TGG | 0.349576 | 5:+15764965 | None:intergenic |
| CATCAATTAATAAATATAAT+AGG | 0.353692 | 5:-15764550 | MsG0580025240.01.T01:three_prime_UTR |
| ACATGATTGAACATGCATTA+GGG | 0.359404 | 5:+15764912 | None:intergenic |
| CAAAGCAAGGTAACCGTGAC+TGG | 0.363196 | 5:-15765152 | MsG0580025240.01.T01:CDS |
| ATAAGGGAATTATGGAACTA+TGG | 0.365707 | 5:+15766317 | None:intergenic |
| AAGAACAAGAATTAAGGAAA+TGG | 0.376478 | 5:-15766161 | MsG0580025240.01.T01:exon |
| GGATGTATGGGACAATATTA+TGG | 0.386593 | 5:+15766275 | None:intergenic |
| GTGTAAAGAAAGAAATAGAT+TGG | 0.390553 | 5:+15766189 | None:intergenic |
| TGGGAATGTGGATAGAAATA+AGG | 0.391392 | 5:-15765132 | MsG0580025240.01.T01:CDS |
| ATGATGTTCATGAAGTATAA+TGG | 0.425822 | 5:+15764704 | None:intergenic |
| TGATGGAAAAGGAGGATGTA+TGG | 0.427682 | 5:+15766262 | None:intergenic |
| ACCAAGTTAGCTTCAACATA+TGG | 0.451325 | 5:+15765065 | None:intergenic |
| TGTAAAGAAAGAAATAGATT+GGG | 0.462344 | 5:+15766190 | None:intergenic |
| GCCATATGTTGAAGCTAACT+TGG | 0.464839 | 5:-15765066 | MsG0580025240.01.T01:CDS |
| AGAACAAGAATTAAGGAAAT+GGG | 0.470739 | 5:-15766160 | MsG0580025240.01.T01:exon |
| TGCATATGCCTGTGTTATGT+AGG | 0.478193 | 5:+15765034 | None:intergenic |
| AATTCTGTTACTAACATGTC+AGG | 0.503481 | 5:-15765929 | MsG0580025240.01.T01:intron |
| CTAAGGAAGGTGCAGAGCAC+AGG | 0.508118 | 5:-15765107 | MsG0580025240.01.T01:CDS |
| AGGGTTATCATCACTGAAGA+AGG | 0.527497 | 5:+15764931 | None:intergenic |
| TGGATAGAAATAAGGTCCTA+AGG | 0.531057 | 5:-15765124 | MsG0580025240.01.T01:CDS |
| TTAGCAATACCAAACCCAAA+TGG | 0.531163 | 5:-15764974 | MsG0580025240.01.T01:three_prime_UTR |
| GCATATGCCTGTGTTATGTA+GGG | 0.531267 | 5:+15765035 | None:intergenic |
| GATGGAAAAGGAGGATGTAT+GGG | 0.536168 | 5:+15766263 | None:intergenic |
| TGTAGGTGTGAAGGAAGTAG+AGG | 0.545264 | 5:-15765186 | MsG0580025240.01.T01:intron |
| AAAGAAACACAAACCCATGC+AGG | 0.560046 | 5:-15766095 | MsG0580025240.01.T01:intron |
| GGTAACCGTGACTGGGAATG+TGG | 0.560905 | 5:-15765144 | MsG0580025240.01.T01:CDS |
| ATATGATAATGTAGGTGTGA+AGG | 0.572793 | 5:-15765195 | MsG0580025240.01.T01:intron |
| TAGAAATAAGGTCCTAAGGA+AGG | 0.572839 | 5:-15765120 | MsG0580025240.01.T01:CDS |
| TCTATCCACATTCCCAGTCA+CGG | 0.577046 | 5:+15765139 | None:intergenic |
| ATATGTTGAAGCTAACTTGG+TGG | 0.579782 | 5:-15765063 | MsG0580025240.01.T01:CDS |
| ATGGCTGTGAAAGAAGAGTT+CGG | 0.593996 | 5:-15765952 | MsG0580025240.01.T01:CDS |
| AAAGCAAGGTAACCGTGACT+GGG | 0.604590 | 5:-15765151 | MsG0580025240.01.T01:CDS |
| ACAAGTTGCACATATTGTAA+CGG | 0.633804 | 5:-15766349 | None:intergenic |
| GGTGAATAGAGAGCAAAGCA+AGG | 0.638066 | 5:-15765165 | MsG0580025240.01.T01:CDS |
| GGCATATCCCTACATAACAC+AGG | 0.656872 | 5:-15765042 | MsG0580025240.01.T01:CDS |
| AAGGTGAAAATGGACTGTGA+TGG | 0.710877 | 5:-15765971 | MsG0580025240.01.T01:CDS |
| ATGTTCATGAAGTATAATGG+TGG | 0.726385 | 5:+15764707 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATCAATTAATAAATATAAT+AGG | - | Chr5:15766313-15766332 | MsG0580025240.01.T01:five_prime_UTR | 10.0% |
| !! | GTTATATAAAAAAAATTCTA+AGG | + | Chr5:15765430-15765449 | None:intergenic | 10.0% |
| !! | ACTTAAAAAGAAAAGAAAAA+GGG | + | Chr5:15764648-15764667 | None:intergenic | 15.0% |
| !! | TACTTAAAAAGAAAAGAAAA+AGG | + | Chr5:15764649-15764668 | None:intergenic | 15.0% |
| !!! | ACCAATTATATACTTTTATA+AGG | + | Chr5:15764566-15764585 | None:intergenic | 15.0% |
| !!! | CCAATTATATACTTTTATAA+GGG | + | Chr5:15764565-15764584 | None:intergenic | 15.0% |
| !! | CAAAAAAAGAACAAGAATTA+AGG | - | Chr5:15764696-15764715 | MsG0580025240.01.T01:three_prime_UTR | 20.0% |
| !! | CTTAAAAAGAAAAGAAAAAG+GGG | + | Chr5:15764647-15764666 | None:intergenic | 20.0% |
| !! | TACATATACATAAATGAGAT+AGG | + | Chr5:15764816-15764835 | None:intergenic | 20.0% |
| !! | TGTAAAGAAAGAAATAGATT+GGG | + | Chr5:15764676-15764695 | None:intergenic | 20.0% |
| !!! | AATCAAATGGCATAATTTTT+AGG | - | Chr5:15764987-15765006 | MsG0580025240.01.T01:exon | 20.0% |
| !!! | ATACTTTTATAAGGGAATTA+TGG | + | Chr5:15764557-15764576 | None:intergenic | 20.0% |
| !!! | CCCTTATAAAAGTATATAAT+TGG | - | Chr5:15764562-15764581 | MsG0580025240.01.T01:three_prime_UTR | 20.0% |
| !!! | TTACAACAATATGATAATGT+AGG | - | Chr5:15765660-15765679 | MsG0580025240.01.T01:intron | 20.0% |
| !!! | TTTCAAGTGAATTTTAAGTA+CGG | + | Chr5:15765078-15765097 | None:intergenic | 20.0% |
| ! | AAGAACAAGAATTAAGGAAA+TGG | - | Chr5:15764702-15764721 | MsG0580025240.01.T01:three_prime_UTR | 25.0% |
| ! | AATGTTCATATTCTCAATGA+AGG | - | Chr5:15765026-15765045 | MsG0580025240.01.T01:CDS | 25.0% |
| ! | AGAACAAGAATTAAGGAAAT+GGG | - | Chr5:15764703-15764722 | MsG0580025240.01.T01:three_prime_UTR | 25.0% |
| ! | ATAAATGAGATAGGATACAT+AGG | + | Chr5:15764807-15764826 | None:intergenic | 25.0% |
| ! | ATGATGTTCATGAAGTATAA+TGG | + | Chr5:15766162-15766181 | None:intergenic | 25.0% |
| ! | CTTTCTTATCTCAAATCAAA+TGG | - | Chr5:15764974-15764993 | MsG0580025240.01.T01:three_prime_UTR | 25.0% |
| ! | GTGTAAAGAAAGAAATAGAT+TGG | + | Chr5:15764677-15764696 | None:intergenic | 25.0% |
| ! | TAAATGAGATAGGATACATA+GGG | + | Chr5:15764806-15764825 | None:intergenic | 25.0% |
| ! | TCATATCTTCATTGATAAGT+GGG | - | Chr5:15765450-15765469 | MsG0580025240.01.T01:intron | 25.0% |
| ! | TCATGAACATCATGAAAAAA+TGG | - | Chr5:15766169-15766188 | MsG0580025240.01.T01:five_prime_UTR | 25.0% |
| ! | TTGATATTTCAAACGATTCT+CGG | - | Chr5:15765351-15765370 | MsG0580025240.01.T01:intron | 25.0% |
| !! | TAGTAATCAAGTTCTGTTTT+TGG | - | Chr5:15766103-15766122 | MsG0580025240.01.T01:CDS | 25.0% |
| !! | TGTTTTCTTTATAGCCAAAT+TGG | - | Chr5:15765595-15765614 | MsG0580025240.01.T01:intron | 25.0% |
| !! | TTTTGTTAGAACAACCAATT+TGG | + | Chr5:15765612-15765631 | None:intergenic | 25.0% |
| !!! | AGTAATCAAGTTCTGTTTTT+GGG | - | Chr5:15766104-15766123 | MsG0580025240.01.T01:CDS | 25.0% |
| !!! | ATTAAGACTTTGAAGCTATT+TGG | - | Chr5:15766044-15766063 | MsG0580025240.01.T01:intron | 25.0% |
| AAATGAGATAGGATACATAG+GGG | + | Chr5:15764805-15764824 | None:intergenic | 30.0% | |
| AACAGTGGAAACAAAAATGA+TGG | + | Chr5:15764621-15764640 | None:intergenic | 30.0% | |
| AATTCTGTTACTAACATGTC+AGG | - | Chr5:15764934-15764953 | MsG0580025240.01.T01:three_prime_UTR | 30.0% | |
| ACATGATTGAACATGCATTA+GGG | + | Chr5:15765954-15765973 | None:intergenic | 30.0% | |
| AGTGGAAATAAAGGTGAAAA+TGG | - | Chr5:15764882-15764901 | MsG0580025240.01.T01:three_prime_UTR | 30.0% | |
| ATAAGGGAATTATGGAACTA+TGG | + | Chr5:15764549-15764568 | None:intergenic | 30.0% | |
| ATCTTATCATCTGTTCCATT+TGG | + | Chr5:15765907-15765926 | None:intergenic | 30.0% | |
| ATGTTCATGAAGTATAATGG+TGG | + | Chr5:15766159-15766178 | None:intergenic | 30.0% | |
| CTCATATCTTCATTGATAAG+TGG | - | Chr5:15765449-15765468 | MsG0580025240.01.T01:intron | 30.0% | |
| CTTATAGACAGTGGAAATAA+AGG | - | Chr5:15764873-15764892 | MsG0580025240.01.T01:three_prime_UTR | 30.0% | |
| GTTGAAACTCTATTGATCAA+CGG | - | Chr5:15765098-15765117 | MsG0580025240.01.T01:CDS | 30.0% | |
| TCTTATCATCTGTTCCATTT+GGG | + | Chr5:15765906-15765925 | None:intergenic | 30.0% | |
| TGATATTTCAAACGATTCTC+GGG | - | Chr5:15765352-15765371 | MsG0580025240.01.T01:intron | 30.0% | |
| TTCATATTCTCAATGAAGGT+GGG | - | Chr5:15765030-15765049 | MsG0580025240.01.T01:CDS | 30.0% | |
| ! | AAATTCTAAGGGCAGATTAT+AGG | + | Chr5:15765418-15765437 | None:intergenic | 30.0% |
| ! | AACAAAAATGATGGAAAAGG+AGG | + | Chr5:15764612-15764631 | None:intergenic | 30.0% |
| ! | AATTCTAAGGGCAGATTATA+GGG | + | Chr5:15765417-15765436 | None:intergenic | 30.0% |
| ! | ATATGATAATGTAGGTGTGA+AGG | - | Chr5:15765668-15765687 | MsG0580025240.01.T01:intron | 30.0% |
| ! | GGAAACAAAAATGATGGAAA+AGG | + | Chr5:15764615-15764634 | None:intergenic | 30.0% |
| ! | GGTGGGTTCTATAATTTATA+TGG | - | Chr5:15765047-15765066 | MsG0580025240.01.T01:CDS | 30.0% |
| !!! | ATGGGTTTGTGTTTCTTTTT+TGG | + | Chr5:15764766-15764785 | None:intergenic | 30.0% |
| !!! | CTTTTTTCTCTTATAGACAG+TGG | - | Chr5:15764864-15764883 | MsG0580025240.01.T01:three_prime_UTR | 30.0% |
| !!! | TGGGTTTGTGTTTCTTTTTT+GGG | + | Chr5:15764765-15764784 | None:intergenic | 30.0% |
| !!! | TGTTTTTTCACATATCCAGA+AGG | + | Chr5:15765864-15765883 | None:intergenic | 30.0% |
| ATATGTTGAAGCTAACTTGG+TGG | - | Chr5:15765800-15765819 | MsG0580025240.01.T01:intron | 35.0% | |
| ATGGTCTCAGCCATAAAAAT+TGG | - | Chr5:15765284-15765303 | MsG0580025240.01.T01:intron | 35.0% | |
| CACATGATTGAACATGCATT+AGG | + | Chr5:15765955-15765974 | None:intergenic | 35.0% | |
| GGATGTATGGGACAATATTA+TGG | + | Chr5:15764591-15764610 | None:intergenic | 35.0% | |
| GTCATGTCTCACTATAAGAA+AGG | + | Chr5:15765157-15765176 | None:intergenic | 35.0% | |
| GTTCATATTCTCAATGAAGG+TGG | - | Chr5:15765029-15765048 | MsG0580025240.01.T01:CDS | 35.0% | |
| TAGAAATAAGGTCCTAAGGA+AGG | - | Chr5:15765743-15765762 | MsG0580025240.01.T01:intron | 35.0% | |
| TATGCAAAAAATGCACCTTC+TGG | - | Chr5:15765846-15765865 | MsG0580025240.01.T01:intron | 35.0% | |
| TGGATAGAAATAAGGTCCTA+AGG | - | Chr5:15765739-15765758 | MsG0580025240.01.T01:intron | 35.0% | |
| TGGGAATGTGGATAGAAATA+AGG | - | Chr5:15765731-15765750 | MsG0580025240.01.T01:intron | 35.0% | |
| TGGTCTCAGCCATAAAAATT+GGG | - | Chr5:15765285-15765304 | MsG0580025240.01.T01:intron | 35.0% | |
| TTAGCAATACCAAACCCAAA+TGG | - | Chr5:15765889-15765908 | MsG0580025240.01.T01:intron | 35.0% | |
| ! | ACCAAGTTAGCTTCAACATA+TGG | + | Chr5:15765801-15765820 | None:intergenic | 35.0% |
| ! | AGACATGACATGATTTGTGT+TGG | - | Chr5:15765168-15765187 | MsG0580025240.01.T01:CDS | 35.0% |
| ! | CAGGAAAAAGAGCAAAGTTT+TGG | - | Chr5:15765775-15765794 | MsG0580025240.01.T01:intron | 35.0% |
| !! | GCTATTTCCCCCAATTTTTA+TGG | + | Chr5:15765297-15765316 | None:intergenic | 35.0% |
| !! | TGTGTTGGTTCAACAAAAAC+AGG | - | Chr5:15765183-15765202 | MsG0580025240.01.T01:CDS | 35.0% |
| !!! | GGGTTTGTGTTTCTTTTTTG+GGG | + | Chr5:15764764-15764783 | None:intergenic | 35.0% |
| AAAGAAACACAAACCCATGC+AGG | - | Chr5:15764768-15764787 | MsG0580025240.01.T01:three_prime_UTR | 40.0% | |
| AAGAAAAAGGGGTTGAACAG+TGG | + | Chr5:15764636-15764655 | None:intergenic | 40.0% | |
| AGGGTTATCATCACTGAAGA+AGG | + | Chr5:15765935-15765954 | None:intergenic | 40.0% | |
| ATAGGGTGAACTGCTTTGAT+AGG | + | Chr5:15765400-15765419 | None:intergenic | 40.0% | |
| ATGGCTGAGACCATGATAAT+TGG | + | Chr5:15765278-15765297 | None:intergenic | 40.0% | |
| ATGGCTGTGAAAGAAGAGTT+CGG | - | Chr5:15764911-15764930 | MsG0580025240.01.T01:three_prime_UTR | 40.0% | |
| GATGGAAAAGGAGGATGTAT+GGG | + | Chr5:15764603-15764622 | None:intergenic | 40.0% | |
| GCATATGCCTGTGTTATGTA+GGG | + | Chr5:15765831-15765850 | None:intergenic | 40.0% | |
| GCCATATGTTGAAGCTAACT+TGG | - | Chr5:15765797-15765816 | MsG0580025240.01.T01:intron | 40.0% | |
| GGTCTCAGCCATAAAAATTG+GGG | - | Chr5:15765286-15765305 | MsG0580025240.01.T01:intron | 40.0% | |
| GTCTCAGCCATAAAAATTGG+GGG | - | Chr5:15765287-15765306 | MsG0580025240.01.T01:intron | 40.0% | |
| TGATGGAAAAGGAGGATGTA+TGG | + | Chr5:15764604-15764623 | None:intergenic | 40.0% | |
| TGCATATGCCTGTGTTATGT+AGG | + | Chr5:15765832-15765851 | None:intergenic | 40.0% | |
| ! | GTTGGTTCAACAAAAACAGG+AGG | - | Chr5:15765186-15765205 | MsG0580025240.01.T01:intron | 40.0% |
| ! | TCATCTGTTCCATTTGGGTT+TGG | + | Chr5:15765901-15765920 | None:intergenic | 40.0% |
| !! | AAGGTGAAAATGGACTGTGA+TGG | - | Chr5:15764892-15764911 | MsG0580025240.01.T01:three_prime_UTR | 40.0% |
| AAAGCAAGGTAACCGTGACT+GGG | - | Chr5:15765712-15765731 | MsG0580025240.01.T01:intron | 45.0% | |
| ATTGTGAGCGTCTACTTTCG+AGG | + | Chr5:15765128-15765147 | None:intergenic | 45.0% | |
| CAAACGATTCTCGGGTATCT+AGG | - | Chr5:15765360-15765379 | MsG0580025240.01.T01:intron | 45.0% | |
| CGACTCGAGACCAATTATCA+TGG | - | Chr5:15765265-15765284 | MsG0580025240.01.T01:intron | 45.0% | |
| GGCATATCCCTACATAACAC+AGG | - | Chr5:15765821-15765840 | MsG0580025240.01.T01:intron | 45.0% | |
| GGCTTGTGAAAAACCTGCAT+GGG | + | Chr5:15764784-15764803 | None:intergenic | 45.0% | |
| GGTGAATAGAGAGCAAAGCA+AGG | - | Chr5:15765698-15765717 | MsG0580025240.01.T01:intron | 45.0% | |
| TCTATCCACATTCCCAGTCA+CGG | + | Chr5:15765727-15765746 | None:intergenic | 45.0% | |
| TGTAGGTGTGAAGGAAGTAG+AGG | - | Chr5:15765677-15765696 | MsG0580025240.01.T01:intron | 45.0% | |
| !! | TTATATAAAAAAAATTCTAA+GGG | + | Chr5:15765429-15765448 | None:intergenic | 5.0% |
| CAAAGCAAGGTAACCGTGAC+TGG | - | Chr5:15765711-15765730 | MsG0580025240.01.T01:intron | 50.0% | |
| GGGCTTGTGAAAAACCTGCA+TGG | + | Chr5:15764785-15764804 | None:intergenic | 50.0% | |
| GGGTGAACTGCTTTGATAGG+CGG | + | Chr5:15765397-15765416 | None:intergenic | 50.0% | |
| TCGGGTATCTAGGTTCACGA+TGG | - | Chr5:15765370-15765389 | MsG0580025240.01.T01:intron | 50.0% | |
| CTGTGCTCTGCACCTTCCTT+AGG | + | Chr5:15765758-15765777 | None:intergenic | 55.0% | |
| GGTAACCGTGACTGGGAATG+TGG | - | Chr5:15765719-15765738 | MsG0580025240.01.T01:intron | 55.0% | |
| ! | CTAAGGAAGGTGCAGAGCAC+AGG | - | Chr5:15765756-15765775 | MsG0580025240.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 15764525 | 15766360 | 15764525 | ID=MsG0580025240.01;Name=MsG0580025240.01 |
| Chr5 | mRNA | 15764525 | 15766360 | 15764525 | ID=MsG0580025240.01.T01;Parent=MsG0580025240.01;Name=MsG0580025240.01.T01;_AED=0.33;_eAED=0.33;_QI=196|1|1|1|1|1|3|473|116 |
| Chr5 | exon | 15764525 | 15765203 | 15764525 | ID=MsG0580025240.01.T01:exon:30212;Parent=MsG0580025240.01.T01 |
| Chr5 | exon | 15765930 | 15766005 | 15765930 | ID=MsG0580025240.01.T01:exon:30211;Parent=MsG0580025240.01.T01 |
| Chr5 | exon | 15766096 | 15766360 | 15766096 | ID=MsG0580025240.01.T01:exon:30210;Parent=MsG0580025240.01.T01 |
| Chr5 | five_prime_UTR | 15766165 | 15766360 | 15766165 | ID=MsG0580025240.01.T01:five_prime_utr;Parent=MsG0580025240.01.T01 |
| Chr5 | CDS | 15766096 | 15766164 | 15766096 | ID=MsG0580025240.01.T01:cds;Parent=MsG0580025240.01.T01 |
| Chr5 | CDS | 15765930 | 15766005 | 15765930 | ID=MsG0580025240.01.T01:cds;Parent=MsG0580025240.01.T01 |
| Chr5 | CDS | 15764998 | 15765203 | 15764998 | ID=MsG0580025240.01.T01:cds;Parent=MsG0580025240.01.T01 |
| Chr5 | three_prime_UTR | 15764525 | 15764997 | 15764525 | ID=MsG0580025240.01.T01:three_prime_utr;Parent=MsG0580025240.01.T01 |
| Gene Sequence |
| Protein sequence |
