AlfalfaGEDB | MsG0180003669.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003669.01

KEGG: K07901
GO: GO:0003924 GO:0005525
PFAM: PF00071

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003669.01.T01	KAF2592179.1	61.765	306	85	6	3	281	46	346	1.11E-108	342
MsG0180003669.01.T01	KAF2592179.1	64.151	159	40	4	135	281	26	179	7.30E-51	191

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003669.01.T01	Q39433	81.538	130	16	2	132	253	60	189	8.39E-64	213

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003669.01.T01	A0A2U1LM85	65.613	253	73	2	3	254	83	322	1.75e-97	314

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0180003669.01	0.806094	9.720706e-50	5.230071e-47
MsG0080047869.01	MsG0180003669.01	0.875565	2.786908e-68	1.307525e-64
MsG0080047940.01	MsG0180003669.01	0.807023	6.187300e-50	3.409275e-47
MsG0080047944.01	MsG0180003669.01	0.832498	9.136887e-56	1.023080e-52
MsG0080047957.01	MsG0180003669.01	0.817585	3.055202e-52	2.233890e-49
MsG0080047959.01	MsG0180003669.01	0.833675	4.654986e-56	5.398597e-53
MsG0080048113.01	MsG0180003669.01	0.835160	1.972671e-56	2.392200e-53
MsG0080048461.01	MsG0180003669.01	0.830793	2.404290e-55	2.559704e-52
MsG0080048462.01	MsG0180003669.01	0.856685	2.722912e-62	6.551257e-59
MsG0080048600.01	MsG0180003669.01	0.801672	8.063060e-49	3.873366e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003669.01.T01	MTR_1g068730	86.154	130	10	2	132	253	60	189	2.92e-68	222
MsG0180003669.01.T01	MTR_1g068730	96.000	75	3	0	3	77	83	157	2.37e-43	155
MsG0180003669.01.T01	MTR_1g068740	85.385	130	11	2	132	253	60	189	1.03e-67	221
MsG0180003669.01.T01	MTR_1g068740	96.000	75	3	0	3	77	83	157	1.96e-43	155
MsG0180003669.01.T01	MTR_7g100020	81.538	130	16	2	132	253	60	189	1.21e-63	210
MsG0180003669.01.T01	MTR_7g100020	74.038	104	25	1	3	104	83	186	3.36e-42	152
MsG0180003669.01.T01	MTR_1g115325	80.769	130	17	2	132	253	60	189	1.78e-63	209
MsG0180003669.01.T01	MTR_1g115325	93.333	75	5	0	3	77	83	157	2.00e-42	153
MsG0180003669.01.T01	MTR_4g063160	79.545	132	17	2	132	253	60	191	1.11e-62	207
MsG0180003669.01.T01	MTR_4g063160	92.105	76	6	0	2	77	84	159	1.06e-42	154

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003669.01.T01	AT3G46060	80.000	130	18	2	132	253	60	189	8.63e-64	211
MsG0180003669.01.T01	AT3G46060	93.333	75	5	0	3	77	83	157	2.15e-42	153
MsG0180003669.01.T01	AT3G46060	80.000	130	18	2	132	253	60	189	8.63e-64	211
MsG0180003669.01.T01	AT3G46060	93.333	75	5	0	3	77	83	157	2.15e-42	153
MsG0180003669.01.T01	AT3G46060	80.000	130	18	2	132	253	60	189	8.63e-64	211
MsG0180003669.01.T01	AT3G46060	93.333	75	5	0	3	77	83	157	2.15e-42	153
MsG0180003669.01.T01	AT5G59840	79.231	130	19	2	132	253	60	189	4.73e-63	209
MsG0180003669.01.T01	AT5G59840	93.333	75	5	0	3	77	83	157	4.90e-42	152
MsG0180003669.01.T01	AT5G03520	77.692	130	21	2	132	253	50	179	9.99e-62	205
MsG0180003669.01.T01	AT5G03520	70.192	104	29	1	3	104	73	176	7.78e-42	151

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Find 214 sgRNAs with CRISPR-Local

Find 2105 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAATGGAGATTGAT+TGG	0.415782	1:+66338625	None:intergenic
AAAATAAAGGGTGTCGGTGA+GGG	0.548302	1:-66309225	MsG0180003669.01.T01:intron
AAAGCAATGCGATCGACCAC+AGG	0.523166	1:+66314360	None:intergenic
AAATTCTAGGTTATAGAAAG+GGG	0.577129	1:-66321879	MsG0180003669.01.T01:intron
AACAGCCACGACTTCGGTTC+TGG	0.365918	1:-66310262	MsG0180003669.01.T01:CDS
AACCAGTTTATTACAACCAT+TGG	0.395231	1:-66336267	MsG0180003669.01.T01:five_prime_UTR
AACTAATATGAATGTAGATG+AGG	0.592652	1:-66323689	MsG0180003669.01.T01:CDS
AACTGCAAAGGGTCAAGCTC+TGG	0.455648	1:-66323833	MsG0180003669.01.T01:CDS
AACTTCAAAGGGTCAAGCTC+TGG	0.455648	1:-66332285	MsG0180003669.01.T01:CDS
AAGGGTGTCGGTGAGGGAGA+TGG	0.430711	1:-66309219	MsG0180003669.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAGTGGAAAAAAAGGGTT+TGG	-	Chr1:66319534-66319553	MsG0180003669.01.T01:intron	30.0%
AAAATCTATTAATAGGCCAC+AGG	+	Chr1:66320971-66320990	None:intergenic	30.0%
AAAGAAAACCAGCAATATCA+AGG	+	Chr1:66315655-66315674	None:intergenic	30.0%
AAAGATGCTAACATACTTAG+AGG	-	Chr1:66335157-66335176	MsG0180003669.01.T01:intron	30.0%
AAATTACAATAACGCCCATA+CGG	+	Chr1:66334563-66334582	None:intergenic	30.0%
AACATTAGCACAATATGACA+TGG	-	Chr1:66312112-66312131	MsG0180003669.01.T01:intron	30.0%
AACCAGTTTATTACAACCAT+TGG	-	Chr1:66311328-66311347	MsG0180003669.01.T01:intron	30.0%
AACCATGCATCACAAATATT+TGG	+	Chr1:66330052-66330071	None:intergenic	30.0%
AACTCAAAGGGATAAAAATG+TGG	+	Chr1:66334965-66334984	None:intergenic	30.0%
AAGAAATGACATAGCAGAAT+TGG	+	Chr1:66324739-66324758	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	66308908	66338709	66308908	ID=MsG0180003669.01;Name=MsG0180003669.01
Chr1	mRNA	66308908	66338709	66308908	ID=MsG0180003669.01.T01;Parent=MsG0180003669.01;Name=MsG0180003669.01.T01;_AED=0.45;_eAED=0.53;_QI=680\|0.27\|0.39\|0.95\|0.95\|0.91\|23\|0\|668
Chr1	exon	66338543	66338709	66338543	ID=MsG0180003669.01.T01:exon:3651;Parent=MsG0180003669.01.T01
Chr1	exon	66338346	66338499	66338346	ID=MsG0180003669.01.T01:exon:3652;Parent=MsG0180003669.01.T01
Chr1	exon	66336247	66336337	66336247	ID=MsG0180003669.01.T01:exon:3653;Parent=MsG0180003669.01.T01
Chr1	exon	66336053	66336152	66336053	ID=MsG0180003669.01.T01:exon:3654;Parent=MsG0180003669.01.T01
Chr1	exon	66335538	66335605	66335538	ID=MsG0180003669.01.T01:exon:3655;Parent=MsG0180003669.01.T01
Chr1	exon	66332936	66333042	66332936	ID=MsG0180003669.01.T01:exon:3656;Parent=MsG0180003669.01.T01
Chr1	exon	66332746	66332811	66332746	ID=MsG0180003669.01.T01:exon:3657;Parent=MsG0180003669.01.T01
Chr1	exon	66332422	66332519	66332422	ID=MsG0180003669.01.T01:exon:3658;Parent=MsG0180003669.01.T01
Chr1	exon	66332254	66332315	66332254	ID=MsG0180003669.01.T01:exon:3659;Parent=MsG0180003669.01.T01
Chr1	exon	66328778	66328925	66328778	ID=MsG0180003669.01.T01:exon:3660;Parent=MsG0180003669.01.T01
Chr1	exon	66326990	66327051	66326990	ID=MsG0180003669.01.T01:exon:3661;Parent=MsG0180003669.01.T01
Chr1	exon	66324298	66324362	66324298	ID=MsG0180003669.01.T01:exon:3662;Parent=MsG0180003669.01.T01
Chr1	exon	66323969	66324066	66323969	ID=MsG0180003669.01.T01:exon:3663;Parent=MsG0180003669.01.T01
Chr1	exon	66323802	66323863	66323802	ID=MsG0180003669.01.T01:exon:3664;Parent=MsG0180003669.01.T01
Chr1	exon	66323627	66323717	66323627	ID=MsG0180003669.01.T01:exon:3665;Parent=MsG0180003669.01.T01
Chr1	exon	66321816	66321892	66321816	ID=MsG0180003669.01.T01:exon:3666;Parent=MsG0180003669.01.T01
Chr1	exon	66314675	66314905	66314675	ID=MsG0180003669.01.T01:exon:3667;Parent=MsG0180003669.01.T01
Chr1	exon	66314353	66314479	66314353	ID=MsG0180003669.01.T01:exon:3668;Parent=MsG0180003669.01.T01
Chr1	exon	66313563	66313656	66313563	ID=MsG0180003669.01.T01:exon:3669;Parent=MsG0180003669.01.T01
Chr1	exon	66310255	66310378	66310255	ID=MsG0180003669.01.T01:exon:3670;Parent=MsG0180003669.01.T01
Chr1	exon	66309979	66310171	66309979	ID=MsG0180003669.01.T01:exon:3671;Parent=MsG0180003669.01.T01
Chr1	exon	66309516	66309586	66309516	ID=MsG0180003669.01.T01:exon:3672;Parent=MsG0180003669.01.T01
Chr1	exon	66308908	66309238	66308908	ID=MsG0180003669.01.T01:exon:3673;Parent=MsG0180003669.01.T01
Chr1	five_prime_UTR	66338543	66338709	66338543	ID=MsG0180003669.01.T01:five_prime_utr;Parent=MsG0180003669.01.T01
Chr1	five_prime_UTR	66338346	66338499	66338346	ID=MsG0180003669.01.T01:five_prime_utr;Parent=MsG0180003669.01.T01
Chr1	five_prime_UTR	66336247	66336337	66336247	ID=MsG0180003669.01.T01:five_prime_utr;Parent=MsG0180003669.01.T01
Chr1	five_prime_UTR	66336053	66336152	66336053	ID=MsG0180003669.01.T01:five_prime_utr;Parent=MsG0180003669.01.T01
Chr1	five_prime_UTR	66335538	66335605	66335538	ID=MsG0180003669.01.T01:five_prime_utr;Parent=MsG0180003669.01.T01
Chr1	five_prime_UTR	66332943	66333042	66332943	ID=MsG0180003669.01.T01:five_prime_utr;Parent=MsG0180003669.01.T01
Chr1	CDS	66332936	66332942	66332936	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66332746	66332811	66332746	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66332422	66332519	66332422	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66332254	66332315	66332254	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66328778	66328925	66328778	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66326990	66327051	66326990	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66324298	66324362	66324298	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66323969	66324066	66323969	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66323802	66323863	66323802	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66323627	66323717	66323627	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66321816	66321892	66321816	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66314675	66314905	66314675	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66314353	66314479	66314353	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66313563	66313656	66313563	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66310255	66310378	66310255	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66309979	66310171	66309979	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66309516	66309586	66309516	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01
Chr1	CDS	66308908	66309238	66308908	ID=MsG0180003669.01.T01:cds;Parent=MsG0180003669.01.T01

Gene Sequence

>MsG0180003669.01.T01

ATGTATTCTTATTACCGTGGAGCAATGGGTATTTTGCTTGTGTATGATGTCACCGATGAGTCGTCTTTTAACAACATCAAGAATTGGATTCGCAACATTGAGCAGCATGCTTCTGACAATGTCAACAAAATATTAGTGGGGAACAAGGCTGACATGGATGAAAGCAAAAGGGCTGTTCCAACTTCAAAGGGTCAAGCTCTGGCAGACGAATACGGCATCAAGTTTTTGAAACTGTGGTTTGGGGGTAAGGTGAGAGTGGTCAACTTGAGTCTTTTGGCAAAATGGCGTTGGCGTATTCTACAACATGATTCTCCTTTGTGGAAAAATGTCCTTGTCAAAAAGTTTGATCGGAGTGGAGTCACTTGGCCGAGAAGTGCTTCTGACCATAGAGCTGATGGTAAGCGAATCAAGTTGCAAATATGGGACACGGCTGGTCAAGAGCGCTTATTACCGTGGAGCAATGGGTATTTGCTTGTGTATGATGTCACCGATGAGTCATCTTTTAACAACATCAAAAATTGGATTCGCAACATTGAGCAGCATGCTTCTGACAATGTCAACAAAAATTTAGTGGGGAACAAGGCCGACATGGATGAAAGCAAAAGGGCTGTTCCAACTGCAAAGGGTCAAGCTCTGGCAGATGAATACGGCATCAAATTTTTCGAACTTGCAAAAACTAATATGAATGTAGATGAGGTTTTCTTTTCAATAGCTCGGGACATCAAACAAAGACTTGCAGAAAGCGACTCAAAGACTGAGGTTATAGAAAGGGGAAACTTTCAAGGCGTTGGATTTGTTCAATCTTTGAAGAGGAGGCTTCAATCGAAGAACTCAAGATGCGGTAATGAAGAATATGAATTTTTCCAACAATTTGGTGTAAATGGATTTTGGAATGTATTGGGACGGCGACGGCGTCATATTGGGTTAATGGTTGTCCGACTGATGATTTTCTTATTGAGCGAGGTTTCTTCGTCAGGAGATCCATTGTCTCCTTTTTTATTTTTGCTTGCCGCAGAAGGTTTTAATGTGCTAATGAACGCGGTGGTGGCTGCTCACCGCTTTGTTGGAGGCGGCGATGGTTATGAATTGTCGAAGGGGTACTGTTCCGTTTACTTACTTGGTTTGCCTATCGGTGGTGATTCAAGGAAACTTAGTTTCTGGAAACCTGTGGTCGATCGCATTGCTTTCTCGCCTTACATTTCTGATAAATGGAGTTGGATTCTAGACCCTTCTGATGGGTACTCAGTTAGGAGTGCTTACCGCTATCTTACTGATTCAGGAGATCCTGGCTTAAGACTCGATGGATTTTACTTATTTGATGAGGTTATGAAGAAGATGAATTTTCTGGTGCTTTGGAGGAAGTGGATTACAGAATGTGTTGGAACAGCCACGACTTCGGTTCTGGTTAATGGGTTCCATGTCCTTATGCAATCTCTGTGGAGGCCGACCTTTTTCATGGGTTTAAAGTGGGGCCGTGAAAATGCATTGGGTCTGACGCACCTTCAATTCGCAGATGATACCTTGATTATAGGAGATAAAAGTTGGTTGAATGTGCGATCGATGCGGGCTGCCTTGTTGATTTTTGAGCAAATGTCTGGTTTAAAGGAGGATGGAGGGTTAGGTGTTAGAAGGTTGTGTGAGTTTAATGTGGCATGTTTTAGGGAAGTGGTGCTGGCGGTGTCGGTGAGGGAGATGGAGGTAGAGGGTGGGTGGTTGATGGAGGAGCCTGGGAGTGGAGAAGGCGTTTGTTGGCTTGGGAGAGGAATCCTTGTCGGAGTGTGCACCCTTCTGTCTAATATTGTTTTGCAGGATTCTATTCTTGACAGGTGGAGGTGGGTACACGATCCTATTAATGGTTATTCTGTTCGAGGAACTTACAAATACCTCACGACGTCGGTTTACTACGTGGGGCGGCCTTTACCTGATGTTGTGTGGCTGAAGCAGGTTCCACTGAAGGTGTCGGTGTTTGTTTGCGACTCCTACACAACAGACTTCGACAAAAGATAA

Protein sequence

>MsG0180003669.01.T01

MYSYYRGAMGILLVYDVTDESSFNNIKNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFLKLWFGGKVRVVNLSLLAKWRWRILQHDSPLWKNVLVKKFDRSGVTWPRSASDHRADGKRIKLQIWDTAGQERLLPWSNGYLLVYDVTDESSFNNIKNWIRNIEQHASDNVNKNLVGNKADMDESKRAVPTAKGQALADEYGIKFFELAKTNMNVDEVFFSIARDIKQRLAESDSKTEVIERGNFQGVGFVQSLKRRLQSKNSRCGNEEYEFFQQFGVNGFWNVLGRRRRHIGLMVVRLMIFLLSEVSSSGDPLSPFLFLLAAEGFNVLMNAVVAAHRFVGGGDGYELSKGYCSVYLLGLPIGGDSRKLSFWKPVVDRIAFSPYISDKWSWILDPSDGYSVRSAYRYLTDSGDPGLRLDGFYLFDEVMKKMNFLVLWRKWITECVGTATTSVLVNGFHVLMQSLWRPTFFMGLKWGRENALGLTHLQFADDTLIIGDKSWLNVRSMRAALLIFEQMSGLKEDGGLGVRRLCEFNVACFREVVLAVSVREMEVEGGWLMEEPGSGEGVCWLGRGILVGVCTLLSNIVLQDSILDRWRWVHDPINGYSVRGTYKYLTTSVYYVGRPLPDVVWLKQVPLKVSVFVCDSYTTDFDKR*