AlfalfaGEDB | MsG0580030180.01

M. sativa cultivar ZhongmuNo.1 / MsG0580030180.01

KEGG: K10638
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030180.01.T01	XP_003618072.2	91.667	60	4	1	12	70	29	88	1.80E-20	94.7

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030180.01.T01	G7K0P6	91.667	60	4	1	12	70	29	88	8.60e-21	94.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001719.01	MsG0580030180.01	0.817970	2.500992e-52	1.848050e-49
MsG0180004828.01	MsG0580030180.01	0.814873	1.234215e-51	8.378760e-49
MsG0180005655.01	MsG0580030180.01	0.814427	1.549386e-51	1.039341e-48
MsG0380014807.01	MsG0580030180.01	0.811201	7.880269e-51	4.847548e-48
MsG0380017331.01	MsG0580030180.01	0.803802	2.929222e-49	1.485844e-46
MsG0580030180.01	MsG0780036553.01	0.837335	5.522869e-57	7.151057e-54
MsG0580030180.01	MsG0780038736.01	0.804574	2.023318e-49	1.046894e-46
MsG0580030180.01	MsG0880044259.01	0.805356	1.388871e-49	7.332433e-47
MsG0280007688.01	MsG0580030180.01	0.805483	1.305876e-49	6.916893e-47
MsG0280009216.01	MsG0580030180.01	0.832664	8.310192e-56	9.351614e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580030180.01.T01	MTR_5g098680	91.667	60	4	1	12	70	29	88	2.18e-24	94.7
MsG0580030180.01.T01	MTR_8g102580	67.647	68	18	3	4	70	16	80	7.47e-23	90.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 18 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGCTGCTCCGTCTGTTGC+GGG	0.263511	5:-107893637	MsG0580030180.01.T01:CDS
CAATCGAGCATTTCACTTGT+TGG	0.315685	5:+107893703	None:intergenic
CTCCGCTATTCGCGCTATTG+AGG	0.444398	5:-107893605	MsG0580030180.01.T01:CDS
CAACAAGTGAAATGCTCGAT+TGG	0.467872	5:-107893702	MsG0580030180.01.T01:CDS
TGCTGCTGCTCCGTCTGTTG+CGG	0.468294	5:-107893638	MsG0580030180.01.T01:CDS
TCACTTGGTTGAGCACAATC+AGG	0.500895	5:+107893673	None:intergenic
AACATGCCATGGTGCTGTGC+AGG	0.512754	5:+107893744	None:intergenic
ATTTCACTTGTTGGAAACAC+AGG	0.519317	5:+107893712	None:intergenic
CTGCTGCTCCGTCTGTTGCG+GGG	0.521108	5:-107893636	MsG0580030180.01.T01:CDS
TGCTGCTCCGTCTGTTGCGG+GGG	0.540490	5:-107893635	MsG0580030180.01.T01:CDS
GTTGGAAACACAGGGAGACA+TGG	0.583385	5:+107893721	None:intergenic
TTCCTCAATAGCGCGAATAG+CGG	0.585677	5:+107893603	None:intergenic
TGACGCTGACGGTGTATGCA+TGG	0.605697	5:-107893808	None:intergenic
GCAGCCGCAACAACATCACT+TGG	0.625060	5:+107893658	None:intergenic
TTTCACTTGTTGGAAACACA+GGG	0.626214	5:+107893713	None:intergenic
TCAACCAAGTGATGTTGTTG+CGG	0.635377	5:-107893662	MsG0580030180.01.T01:CDS
GGGAGACATGGAACATGCCA+TGG	0.637125	5:+107893733	None:intergenic
GCAAAACCTGCACAGCACCA+TGG	0.665753	5:-107893750	MsG0580030180.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTTCACTTGTTGGAAACAC+AGG	+	Chr5:107893684-107893703	None:intergenic	35.0%
!	TTTCACTTGTTGGAAACACA+GGG	+	Chr5:107893683-107893702	None:intergenic	35.0%
!!	AGGTTTTGCAATGAAGTGTT+CGG	+	Chr5:107893632-107893651	None:intergenic	35.0%
	CAACAAGTGAAATGCTCGAT+TGG	-	Chr5:107893691-107893710	MsG0580030180.01.T01:CDS	40.0%
	CAATCGAGCATTTCACTTGT+TGG	+	Chr5:107893693-107893712	None:intergenic	40.0%
	TCAACCAAGTGATGTTGTTG+CGG	-	Chr5:107893731-107893750	MsG0580030180.01.T01:CDS	40.0%
	TCACTTGGTTGAGCACAATC+AGG	+	Chr5:107893723-107893742	None:intergenic	45.0%
	TTCCTCAATAGCGCGAATAG+CGG	+	Chr5:107893793-107893812	None:intergenic	45.0%
	GTTGGAAACACAGGGAGACA+TGG	+	Chr5:107893675-107893694	None:intergenic	50.0%
	ACAAGATCCCCCGCAACAGA+CGG	+	Chr5:107893768-107893787	None:intergenic	55.0%
	CTCCGCTATTCGCGCTATTG+AGG	-	Chr5:107893788-107893807	MsG0580030180.01.T01:CDS	55.0%
	GCAAAACCTGCACAGCACCA+TGG	-	Chr5:107893643-107893662	MsG0580030180.01.T01:CDS	55.0%
	GCAGCCGCAACAACATCACT+TGG	+	Chr5:107893738-107893757	None:intergenic	55.0%
	GGGAGACATGGAACATGCCA+TGG	+	Chr5:107893663-107893682	None:intergenic	55.0%
!!	AACATGCCATGGTGCTGTGC+AGG	+	Chr5:107893652-107893671	None:intergenic	55.0%
	TGCTGCTGCTCCGTCTGTTG+CGG	-	Chr5:107893755-107893774	MsG0580030180.01.T01:CDS	60.0%
	CTGCTGCTCCGTCTGTTGCG+GGG	-	Chr5:107893757-107893776	MsG0580030180.01.T01:CDS	65.0%
	GCTGCTGCTCCGTCTGTTGC+GGG	-	Chr5:107893756-107893775	MsG0580030180.01.T01:CDS	65.0%
	TGCTGCTCCGTCTGTTGCGG+GGG	-	Chr5:107893758-107893777	MsG0580030180.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	107893600	107893815	107893600	ID=MsG0580030180.01;Name=MsG0580030180.01
Chr5	mRNA	107893600	107893815	107893600	ID=MsG0580030180.01.T01;Parent=MsG0580030180.01;Name=MsG0580030180.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|71
Chr5	exon	107893600	107893815	107893600	ID=MsG0580030180.01.T01:exon:17581;Parent=MsG0580030180.01.T01
Chr5	CDS	107893600	107893815	107893600	ID=MsG0580030180.01.T01:cds;Parent=MsG0580030180.01.T01

Gene Sequence	>MsG0580030180.01.T01 ATGCATGGTTGCAAAACAAACATCAGAAACCGAACACTTCATTGCAAAACCTGCACAGCACCATGGCATGTTCCATGTCTCCCTGTGTTTCCAACAAGTGAAATGCTCGATTGGTTGTGTCCTGATTGTGCTCAACCAAGTGATGTTGTTGCGGCTGCTGCTGCTCCGTCTGTTGCGGGGGATCTTGTCTCCGCTATTCGCGCTATTGAGGAATGA
Protein sequence	>MsG0580030180.01.T01 MHGCKTNIRNRTLHCKTCTAPWHVPCLPVFPTSEMLDWLCPDCAQPSDVVAAAAAPSVAGDLVSAIRAIEE*

Copyright © Liu Lab , TPR, XTBG, China.

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