AlfalfaGEDB | MsG0280007688.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007688.01

KEGG: K00430
GO: GO:0004601 GO:0006979 GO:0020037 GO:0042744 GO:0055114
PFAM: PF00141

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007688.01.T01	XP_003594502.1	82.436	353	58	2	1	349	1	353	0	583

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007688.01.T01	Q9LEH3	58.896	326	129	2	3	327	2	323	1.83E-126	368

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007688.01.T01	G7IJV0	82.436	353	58	2	1	349	1	353	0.0	583

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0280007688.01	0.808223	3.441063e-50	1.956517e-47
MsG0080048258.01	MsG0280007688.01	0.805874	1.081121e-49	5.784307e-47
MsG0080048797.01	MsG0280007688.01	0.813610	2.346011e-51	1.539247e-48
MsG0180001290.01	MsG0280007688.01	0.816114	6.535376e-52	4.590039e-49
MsG0180001293.01	MsG0280007688.01	0.822445	2.360322e-53	1.976033e-50
MsG0180001719.01	MsG0280007688.01	0.847502	1.110508e-59	1.977919e-56
MsG0180002134.01	MsG0280007688.01	0.805317	1.414833e-49	7.462056e-47
MsG0180002218.01	MsG0280007688.01	0.802821	4.678063e-49	2.314118e-46
MsG0180002397.01	MsG0280007688.01	0.800917	1.150650e-48	5.423534e-46
MsG0180003504.01	MsG0280007688.01	0.848072	7.734903e-60	1.402573e-56

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PPI

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Gene1	Gene2	Type
MsG0280007688.01	MsG0880043112.01	PPI
MsG0280007688.01	MsG0280010150.01	PPI
MsG0280007688.01	MsG0680032464.01	PPI
MsG0280009597.01	MsG0280007688.01	PPI
MsG0280010097.01	MsG0280007688.01	PPI
MsG0280010152.01	MsG0280007688.01	PPI
MsG0280011128.01	MsG0280007688.01	PPI
MsG0380016158.01	MsG0280007688.01	PPI
MsG0680032461.01	MsG0280007688.01	PPI
MsG0880047383.01	MsG0280007688.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007688.01.T01	MTR_2g029910	82.436	353	58	2	1	349	1	353	0.0	583
MsG0280007688.01.T01	MTR_2g029850	78.286	350	72	2	4	349	3	352	0.0	538
MsG0280007688.01.T01	MTR_2g029820	67.139	353	109	1	4	349	3	355	2.27e-177	496
MsG0280007688.01.T01	MTR_2g029800	68.091	351	107	1	4	349	3	353	1.14e-174	489
MsG0280007688.01.T01	MTR_2g029860	71.347	349	93	3	7	349	6	353	2.87e-174	488
MsG0280007688.01.T01	MTR_2g029815	65.429	350	117	1	4	349	3	352	2.99e-169	475
MsG0280007688.01.T01	MTR_2g029740	67.372	331	108	0	4	334	3	333	1.76e-162	458
MsG0280007688.01.T01	MTR_2g028980	64.387	351	119	2	4	348	3	353	1.53e-161	456
MsG0280007688.01.T01	MTR_2g029830	70.192	312	93	0	23	334	21	332	3.84e-161	455
MsG0280007688.01.T01	MTR_2g029750	66.163	331	112	0	4	334	3	333	9.14e-160	451

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007688.01.T01	AT5G06720	56.151	317	139	0	15	331	17	333	3.87e-124	360
MsG0280007688.01.T01	AT5G06730	53.709	337	152	1	15	347	18	354	2.82e-123	359
MsG0280007688.01.T01	AT2G38380	55.247	324	142	2	26	348	27	348	1.07e-121	355
MsG0280007688.01.T01	AT2G38390	53.704	324	147	2	26	348	27	348	3.02e-118	346
MsG0280007688.01.T01	AT3G49120	53.374	326	150	2	26	349	28	353	9.50e-115	337
MsG0280007688.01.T01	AT3G32980	53.067	326	151	2	26	349	27	352	2.84e-113	333
MsG0280007688.01.T01	AT3G49110	53.067	326	151	2	26	349	29	354	5.62e-112	330
MsG0280007688.01.T01	AT4G08770	51.411	319	153	2	26	342	20	338	2.70e-110	325
MsG0280007688.01.T01	AT4G08780	50.613	326	159	2	26	349	20	345	3.96e-110	325
MsG0280007688.01.T01	AT5G66390	46.108	334	176	2	1	331	1	333	8.90e-104	308

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Find 76 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTATCTCTTCTTCCTAA+TGG	0.298706	2:+19231605	None:intergenic
AAAGTGAAGCCTAATAAAAC+TGG	0.387546	2:+19232970	None:intergenic
AAATTGGTGAGTGTGCTATT+AGG	0.482129	2:+19230904	None:intergenic
AAGACAAGTTTGCATGAGAA+AGG	0.424864	2:-19230567	MsG0280007688.01.T01:three_prime_UTR
AAGCTTCAAAGAAAAGAGTT+TGG	0.356755	2:+19230740	None:intergenic
AAGGCCTCTATGATTAAAAT+GGG	0.441216	2:-19230715	MsG0280007688.01.T01:CDS
AAGTAGGTTGTGTTAAGAGT+TGG	0.473975	2:+19230961	None:intergenic
AAGTTGTGCATTTGAAGAGA+AGG	0.544272	2:+19233087	None:intergenic
ACAATTGGTAGAGGTCAATG+TGG	0.678112	2:-19231033	MsG0280007688.01.T01:CDS
ACATACATGTGCTCTTGTGC+TGG	0.468347	2:-19230513	MsG0280007688.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTATCTCTTCTTCCTAA+TGG	+	Chr2:19231885-19231904	None:intergenic	30.0%
AAAGTGAAGCCTAATAAAAC+TGG	+	Chr2:19230520-19230539	None:intergenic	30.0%
AAGCTTCAAAGAAAAGAGTT+TGG	+	Chr2:19232750-19232769	None:intergenic	30.0%
AAGGCCTCTATGATTAAAAT+GGG	-	Chr2:19232772-19232791	MsG0280007688.01.T01:intron	30.0%
AATTTACAACTGTCTCTAAC+GGG	-	Chr2:19232316-19232335	MsG0280007688.01.T01:intron	30.0%
ATATTAGGTGCTCATACAAT+TGG	-	Chr2:19232439-19232458	MsG0280007688.01.T01:intron	30.0%
CAATTTACAACTGTCTCTAA+CGG	-	Chr2:19232315-19232334	MsG0280007688.01.T01:intron	30.0%
CCAAATAAAAACTCCATAAG+AGG	-	Chr2:19230887-19230906	MsG0280007688.01.T01:CDS	30.0%
CCACATATACATACATAATG+AGG	+	Chr2:19232393-19232412	None:intergenic	30.0%
CCTAGAAAACACTCTTTATT+GGG	+	Chr2:19232952-19232971	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	19230333	19233176	19230333	ID=MsG0280007688.01;Name=MsG0280007688.01
Chr2	mRNA	19230333	19233176	19230333	ID=MsG0280007688.01.T01;Parent=MsG0280007688.01;Name=MsG0280007688.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|0.66\|0.5\|1\|1\|1\|4\|264\|349
Chr2	exon	19230333	19231063	19230333	ID=MsG0280007688.01.T01:exon:10152;Parent=MsG0280007688.01.T01
Chr2	exon	19231486	19231651	19231486	ID=MsG0280007688.01.T01:exon:10151;Parent=MsG0280007688.01.T01
Chr2	exon	19232494	19232685	19232494	ID=MsG0280007688.01.T01:exon:10150;Parent=MsG0280007688.01.T01
Chr2	exon	19232952	19233176	19232952	ID=MsG0280007688.01.T01:exon:10149;Parent=MsG0280007688.01.T01
Chr2	CDS	19232952	19233176	19232952	ID=MsG0280007688.01.T01:cds;Parent=MsG0280007688.01.T01
Chr2	CDS	19232494	19232685	19232494	ID=MsG0280007688.01.T01:cds;Parent=MsG0280007688.01.T01
Chr2	CDS	19231486	19231651	19231486	ID=MsG0280007688.01.T01:cds;Parent=MsG0280007688.01.T01
Chr2	CDS	19230597	19231063	19230597	ID=MsG0280007688.01.T01:cds;Parent=MsG0280007688.01.T01
Chr2	three_prime_UTR	19230333	19230596	19230333	ID=MsG0280007688.01.T01:three_prime_utr;Parent=MsG0280007688.01.T01

Gene Sequence

>MsG0280007688.01.T01

ATGAACTCTTCTTTTCATCTCATATTGGCAGCTTTGTGCTGTGTAGTGGTTGTGCTTGGAGGGTTTCCCTTCTCTTCAAATGCACAACTTGATCCTAACTTCTACAAGGACACTTGTCCAAATGTCACTTCCATTGTCAAACAAGTCTTAGCAAACGTTTCTCAGACAGATCCTCGTATGCTTGCCAGTTTTATTAGGCTTCACTTTCATGATTGTTTTGTTCAAGGCTGTGATGCATCAATCTTGTTAAATGACACTGATACTATCGTGAGCGAGCAAGGAGCTAGGCCAAATAAAAACTCCATAAGAGGTTTGGATGTTGTGAATCAAATCAAAGACGCAGTGGAACTTGCTTGTCCTAGTGTTGTGTCTTGTGCGGATATTCTTGCACTTGGTGCTAATATTTCCACAGTTCTGGCTCTTGGTCCTGATTGGGAAGTTCCATTAGGAAGAAGAGATAGTTTGAATGCAAATCAAACTCTTGCTAATTCAAACCTTCCTGGTCCCCAATTTGATCTTACTCAGCTAATATCCTCCTTTGCTAAACAAGGTCTCAACATTACTGATCTTGTTGCACTTTCAGGTGCTCATACAATTGGTAGAGGTCAATGTGGATTGTTCTTTGATCGATTATACAATTTCAACGATACTAGAAAACCCGATCCAACTCTTAACACAACCTACTTACAAACATTGAGAACAATATGTCCCATTGATGGACCTAATAGCACACTCACCAATTTGGACCCAACCACTCCAGATACATTCGACTCGAGCTACTACTCAAACCTAAGGATTCAAAAGGGCTTGTTTCAGAGTGACCAAGAATTGTTTTCCACAAGCGGCGCAAACACCACTGCCATTGTCAATAGTTTCAACAACAACCAAACTCTTTTCTTTGAAGCTTTTAAGGCCTCTATGATTAAAATGGGTAATATTGGAGTGTTAAATGAAACTCAAGGTGAAGTTAGAACACACTGTAACTTTGTTAATACTGTTAGCCTTGCTACCAAAGTCACCAAGGAAGATGGTACTGTTAGCTCATTCTAA

Protein sequence

>MsG0280007688.01.T01

MNSSFHLILAALCCVVVVLGGFPFSSNAQLDPNFYKDTCPNVTSIVKQVLANVSQTDPRMLASFIRLHFHDCFVQGCDASILLNDTDTIVSEQGARPNKNSIRGLDVVNQIKDAVELACPSVVSCADILALGANISTVLALGPDWEVPLGRRDSLNANQTLANSNLPGPQFDLTQLISSFAKQGLNITDLVALSGAHTIGRGQCGLFFDRLYNFNDTRKPDPTLNTTYLQTLRTICPIDGPNSTLTNLDPTTPDTFDSSYYSNLRIQKGLFQSDQELFSTSGANTTAIVNSFNNNQTLFFEAFKASMIKMGNIGVLNETQGEVRTHCNFVNTVSLATKVTKEDGTVSSF*