Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030634.01.T01 | XP_013450888.1 | 93.22 | 59 | 4 | 0 | 1 | 59 | 68 | 126 | 1.06E-29 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030634.01.T01 | Q67YE6 | 55.932 | 59 | 26 | 0 | 1 | 59 | 68 | 126 | 2.70E-15 | 70.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030634.01.T01 | A0A396H9D4 | 93.220 | 59 | 4 | 0 | 1 | 59 | 113 | 171 | 1.26e-29 | 115 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004777.01 | MsG0680030634.01 | 0.828232 | 1.007869e-54 | 9.957771e-52 |
| MsG0480023518.01 | MsG0680030634.01 | 0.844861 | 5.813856e-59 | 9.511846e-56 |
| MsG0580025440.01 | MsG0680030634.01 | 0.804931 | 1.704499e-49 | 8.901594e-47 |
| MsG0680030634.01 | MsG0780041177.01 | 0.815549 | 8.731441e-52 | 6.038313e-49 |
| MsG0680030634.01 | MsG0880045818.01 | 0.812645 | 3.819599e-51 | 2.441892e-48 |
| MsG0280010521.01 | MsG0680030634.01 | 0.808199 | 3.483347e-50 | 1.979297e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030634.01.T01 | MTR_6g008210 | 93.220 | 59 | 4 | 0 | 1 | 59 | 68 | 126 | 1.29e-33 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030634.01.T01 | AT3G28210 | 55.932 | 59 | 26 | 0 | 1 | 59 | 68 | 126 | 2.75e-16 | 70.1 |
| MsG0680030634.01.T01 | AT3G28210 | 55.932 | 59 | 26 | 0 | 1 | 59 | 68 | 126 | 2.75e-16 | 70.1 |
Find 22 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAAGAAACATCGTAGTTC+AGG | 0.309481 | 6:+6433003 | MsG0680030634.01.T01:CDS |
| ATCTCCTTGCAACGCTTCAC+GGG | 0.399170 | 6:-6433063 | None:intergenic |
| GTTGGTTTCTTCTTCTTGCT+AGG | 0.399693 | 6:-6433036 | None:intergenic |
| AATCTCCTTGCAACGCTTCA+CGG | 0.405276 | 6:-6433064 | None:intergenic |
| TCTGTAAAACTTGCCACATT+AGG | 0.413892 | 6:+6433109 | MsG0680030634.01.T01:CDS |
| TGAAGAATGCTCCATGTCTA+TGG | 0.426712 | 6:+6432942 | None:intergenic |
| TTGGTTTCTTCTTCTTGCTA+GGG | 0.455380 | 6:-6433035 | None:intergenic |
| CAACGCTTCACGGGGCAAGT+TGG | 0.457687 | 6:-6433054 | None:intergenic |
| TAAAGAAACATCGTAGTTCA+GGG | 0.514380 | 6:+6433004 | MsG0680030634.01.T01:CDS |
| TATGGAGATCACAGGCAACA+TGG | 0.561346 | 6:+6432960 | MsG0680030634.01.T01:CDS |
| TGCAACAGCAGTTGTTGATG+AGG | 0.577728 | 6:-6433188 | None:intergenic |
| GCTGATCATGCTTGTAGTAG+AGG | 0.580042 | 6:+6433155 | MsG0680030634.01.T01:CDS |
| AAGCATGATCAGCAGCAAAA+CGG | 0.580672 | 6:-6433145 | None:intergenic |
| CAACTGCTGTTGCAAATGCA+AGG | 0.582464 | 6:+6433197 | MsG0680030634.01.T01:CDS |
| CAGCAGCAAAACGGTGCTTG+AGG | 0.584113 | 6:-6433136 | None:intergenic |
| TCCATGTCTATGGAGATCAC+AGG | 0.587969 | 6:+6432952 | None:intergenic |
| TGCTTGAGGCACACCTAATG+TGG | 0.589537 | 6:-6433122 | None:intergenic |
| ATGGAGATCACAGGCAACAT+GGG | 0.594572 | 6:+6432961 | MsG0680030634.01.T01:CDS |
| TTGCCCCGTGAAGCGTTGCA+AGG | 0.613440 | 6:+6433059 | MsG0680030634.01.T01:CDS |
| GCCTGTGATCTCCATAGACA+TGG | 0.623654 | 6:-6432953 | None:intergenic |
| TCTCCTTGCAACGCTTCACG+GGG | 0.654277 | 6:-6433062 | None:intergenic |
| CAACATGGGAGAAAACGAAG+AGG | 0.737384 | 6:+6432975 | MsG0680030634.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAAGAAACATCGTAGTTCA+GGG | + | Chr6:6433004-6433023 | MsG0680030634.01.T01:CDS | 30.0% | |
| TTAAAGAAACATCGTAGTTC+AGG | + | Chr6:6433003-6433022 | MsG0680030634.01.T01:CDS | 30.0% | |
| TCTGTAAAACTTGCCACATT+AGG | + | Chr6:6433109-6433128 | MsG0680030634.01.T01:CDS | 35.0% | |
| TTGGTTTCTTCTTCTTGCTA+GGG | - | Chr6:6433038-6433057 | None:intergenic | 35.0% | |
| AAGCATGATCAGCAGCAAAA+CGG | - | Chr6:6433148-6433167 | None:intergenic | 40.0% | |
| ! | GTTGGTTTCTTCTTCTTGCT+AGG | - | Chr6:6433039-6433058 | None:intergenic | 40.0% |
| AATCTCCTTGCAACGCTTCA+CGG | - | Chr6:6433067-6433086 | None:intergenic | 45.0% | |
| ATGGAGATCACAGGCAACAT+GGG | + | Chr6:6432961-6432980 | MsG0680030634.01.T01:CDS | 45.0% | |
| CAACATGGGAGAAAACGAAG+AGG | + | Chr6:6432975-6432994 | MsG0680030634.01.T01:CDS | 45.0% | |
| CAACTGCTGTTGCAAATGCA+AGG | + | Chr6:6433197-6433216 | MsG0680030634.01.T01:CDS | 45.0% | |
| GCTGATCATGCTTGTAGTAG+AGG | + | Chr6:6433155-6433174 | MsG0680030634.01.T01:CDS | 45.0% | |
| TATGGAGATCACAGGCAACA+TGG | + | Chr6:6432960-6432979 | MsG0680030634.01.T01:CDS | 45.0% | |
| TGCAACAGCAGTTGTTGATG+AGG | - | Chr6:6433191-6433210 | None:intergenic | 45.0% | |
| ATCTCCTTGCAACGCTTCAC+GGG | - | Chr6:6433066-6433085 | None:intergenic | 50.0% | |
| TGCTTGAGGCACACCTAATG+TGG | - | Chr6:6433125-6433144 | None:intergenic | 50.0% | |
| CAGCAGCAAAACGGTGCTTG+AGG | - | Chr6:6433139-6433158 | None:intergenic | 55.0% | |
| TCTCCTTGCAACGCTTCACG+GGG | - | Chr6:6433065-6433084 | None:intergenic | 55.0% | |
| CAACGCTTCACGGGGCAAGT+TGG | - | Chr6:6433057-6433076 | None:intergenic | 60.0% | |
| !! | TTGCCCCGTGAAGCGTTGCA+AGG | + | Chr6:6433059-6433078 | MsG0680030634.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 6432955 | 6433227 | 6432955 | ID=MsG0680030634.01;Name=MsG0680030634.01 |
| Chr6 | mRNA | 6432955 | 6433227 | 6432955 | ID=MsG0680030634.01.T01;Parent=MsG0680030634.01;Name=MsG0680030634.01.T01;_AED=0.49;_eAED=0.58;_QI=0|-1|0|1|-1|0|1|0|90 |
| Chr6 | exon | 6432955 | 6433227 | 6432955 | ID=MsG0680030634.01.T01:exon:6192;Parent=MsG0680030634.01.T01 |
| Chr6 | CDS | 6432955 | 6433227 | 6432955 | ID=MsG0680030634.01.T01:cds;Parent=MsG0680030634.01.T01 |
| Gene Sequence |
| Protein sequence |
