Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004777.01.T01 | XP_003591381.1 | 89.394 | 330 | 27 | 3 | 1 | 327 | 1 | 325 | 0 | 524 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048904.01 | MsG0180004777.01 | 0.839597 | 1.440884e-57 | 1.999487e-54 |
| MsG0080048978.01 | MsG0180004777.01 | -0.800410 | 1.460204e-48 | 6.794456e-46 |
| MsG0080049066.01 | MsG0180004777.01 | -0.804622 | 1.977767e-49 | 1.024547e-46 |
| MsG0080049133.01 | MsG0180004777.01 | -0.806308 | 8.761102e-50 | 4.739544e-47 |
| MsG0180000194.01 | MsG0180004777.01 | -0.809041 | 2.301631e-50 | 1.337087e-47 |
| MsG0180000322.01 | MsG0180004777.01 | -0.846186 | 2.543580e-59 | 4.342137e-56 |
| MsG0180000440.01 | MsG0180004777.01 | 0.805866 | 1.085285e-49 | 5.805478e-47 |
| MsG0180000762.01 | MsG0180004777.01 | 0.808511 | 2.988002e-50 | 1.711716e-47 |
| MsG0180001111.01 | MsG0180004777.01 | -0.815327 | 9.784837e-52 | 6.725681e-49 |
| MsG0180001113.01 | MsG0180004777.01 | -0.807443 | 5.042552e-50 | 2.808941e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004777.01.T01 | MTR_1g086790 | 89.394 | 330 | 27 | 3 | 1 | 327 | 1 | 325 | 0.0 | 524 |
| MsG0180004777.01.T01 | MTR_7g109800 | 58.974 | 312 | 80 | 17 | 27 | 327 | 21 | 295 | 1.09e-88 | 268 |
| MsG0180004777.01.T01 | MTR_2g033820 | 47.923 | 313 | 123 | 10 | 44 | 327 | 71 | 372 | 6.86e-56 | 186 |
| MsG0180004777.01.T01 | MTR_5g043880 | 47.236 | 199 | 70 | 5 | 134 | 323 | 98 | 270 | 4.34e-45 | 155 |
| MsG0180004777.01.T01 | MTR_4g094588 | 46.119 | 219 | 91 | 7 | 114 | 324 | 133 | 332 | 9.35e-44 | 153 |
| MsG0180004777.01.T01 | MTR_5g091390 | 80.769 | 78 | 15 | 0 | 146 | 223 | 143 | 220 | 3.58e-41 | 145 |
| MsG0180004777.01.T01 | MTR_4g122530 | 39.301 | 229 | 74 | 7 | 144 | 319 | 192 | 408 | 3.70e-38 | 140 |
| MsG0180004777.01.T01 | MTR_7g079010 | 48.387 | 155 | 62 | 4 | 67 | 217 | 58 | 198 | 9.30e-35 | 126 |
| MsG0180004777.01.T01 | MTR_3g104750 | 66.667 | 78 | 26 | 0 | 141 | 218 | 85 | 162 | 1.21e-31 | 116 |
| MsG0180004777.01.T01 | MTR_7g028415 | 71.429 | 70 | 20 | 0 | 148 | 217 | 81 | 150 | 5.04e-31 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004777.01.T01 | AT2G47260 | 48.024 | 329 | 130 | 8 | 31 | 327 | 18 | 337 | 2.87e-69 | 219 |
| MsG0180004777.01.T01 | AT4G18170 | 77.000 | 100 | 23 | 0 | 124 | 223 | 136 | 235 | 1.55e-50 | 171 |
| MsG0180004777.01.T01 | AT5G49520 | 46.718 | 259 | 113 | 7 | 79 | 323 | 137 | 384 | 2.64e-49 | 169 |
| MsG0180004777.01.T01 | AT1G29860 | 51.087 | 184 | 68 | 4 | 144 | 327 | 120 | 281 | 2.96e-49 | 166 |
| MsG0180004777.01.T01 | AT3G62340 | 36.877 | 301 | 147 | 7 | 27 | 327 | 19 | 276 | 5.79e-46 | 157 |
| MsG0180004777.01.T01 | AT1G69310 | 63.478 | 115 | 36 | 1 | 108 | 216 | 89 | 203 | 3.33e-43 | 150 |
| MsG0180004777.01.T01 | AT1G69310 | 63.478 | 115 | 36 | 1 | 108 | 216 | 89 | 203 | 3.33e-43 | 150 |
| MsG0180004777.01.T01 | AT1G69310 | 63.478 | 115 | 36 | 1 | 108 | 216 | 89 | 203 | 3.33e-43 | 150 |
| MsG0180004777.01.T01 | AT5G46350 | 77.647 | 85 | 18 | 1 | 146 | 229 | 169 | 253 | 5.44e-43 | 151 |
| MsG0180004777.01.T01 | AT1G69310 | 60.000 | 115 | 36 | 2 | 108 | 216 | 89 | 199 | 1.31e-37 | 135 |
Find 87 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCCACCCGAGACTACAC+CGG | 0.638946 | 1:+82668393 | None:intergenic |
| AACTCCGGCAAAGGCGGAAC+GGG | 0.372094 | 1:+82668432 | None:intergenic |
| AAGCGTGTTTGATTTCTGTG+AGG | 0.717658 | 1:-82669567 | MsG0180004777.01.T01:CDS |
| AAGGCGGAACGGGACATAGT+TGG | 0.509083 | 1:+82668442 | None:intergenic |
| AAGGGACAACTGCATTTCTT+AGG | 0.222393 | 1:-82668189 | MsG0180004777.01.T01:CDS |
| AAGTAGTTGTTGATGTTGAT+AGG | 0.343522 | 1:+82668309 | None:intergenic |
| AAGTTCTTCTTTAGGGTTTA+TGG | 0.122782 | 1:-82669537 | MsG0180004777.01.T01:CDS |
| AATCCAAATCCTTCATTTGT+AGG | 0.298705 | 1:+82669698 | None:intergenic |
| AATGATGAAGAATTGTTATA+AGG | 0.284749 | 1:+82668259 | None:intergenic |
| ACAAAGCTGTACTCTCTCTT+GGG | 0.501900 | 1:+82668216 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGTAGTTGTTGATGTTGAT+AGG | + | Chr1:82669353-82669372 | None:intergenic | 30.0% | |
| AATCCAAATCCTTCATTTGT+AGG | + | Chr1:82667964-82667983 | None:intergenic | 30.0% | |
| AGTAGTTGTTGATGTTGATA+GGG | + | Chr1:82669352-82669371 | None:intergenic | 30.0% | |
| ATAACCATTTAGCAAAGATG+AGG | + | Chr1:82668064-82668083 | None:intergenic | 30.0% | |
| ATTATTGTACTGTACCATGA+TGG | - | Chr1:82669746-82669765 | MsG0180004777.01.T01:five_prime_UTR | 30.0% | |
| ATTGTAGTCACAACATATGA+AGG | - | Chr1:82669175-82669194 | MsG0180004777.01.T01:intron | 30.0% | |
| CAAAAAAAGTGAATGGTTGT+TGG | - | Chr1:82668602-82668621 | MsG0180004777.01.T01:intron | 30.0% | |
| CTACATGGAAAAAGATAAAG+TGG | + | Chr1:82669712-82669731 | None:intergenic | 30.0% | |
| CTATGTGTTTATGTTTGTGT+AGG | - | Chr1:82669080-82669099 | MsG0180004777.01.T01:intron | 30.0% | |
| CTGGTTGTATATATGTGATT+AGG | - | Chr1:82668814-82668833 | MsG0180004777.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 82667876 | 82669805 | 82667876 | ID=MsG0180004777.01;Name=MsG0180004777.01 |
| Chr1 | mRNA | 82667876 | 82669805 | 82667876 | ID=MsG0180004777.01.T01;Parent=MsG0180004777.01;Name=MsG0180004777.01.T01;_AED=0.18;_eAED=0.18;_QI=127|1|1|1|1|1|3|259|327 |
| Chr1 | exon | 82667876 | 82668579 | 82667876 | ID=MsG0180004777.01.T01:exon:8608;Parent=MsG0180004777.01.T01 |
| Chr1 | exon | 82668702 | 82668845 | 82668702 | ID=MsG0180004777.01.T01:exon:8607;Parent=MsG0180004777.01.T01 |
| Chr1 | exon | 82669284 | 82669805 | 82669284 | ID=MsG0180004777.01.T01:exon:8606;Parent=MsG0180004777.01.T01 |
| Chr1 | five_prime_UTR | 82669679 | 82669805 | 82669679 | ID=MsG0180004777.01.T01:five_prime_utr;Parent=MsG0180004777.01.T01 |
| Chr1 | CDS | 82669284 | 82669678 | 82669284 | ID=MsG0180004777.01.T01:cds;Parent=MsG0180004777.01.T01 |
| Chr1 | CDS | 82668702 | 82668845 | 82668702 | ID=MsG0180004777.01.T01:cds;Parent=MsG0180004777.01.T01 |
| Chr1 | CDS | 82668135 | 82668579 | 82668135 | ID=MsG0180004777.01.T01:cds;Parent=MsG0180004777.01.T01 |
| Chr1 | three_prime_UTR | 82667876 | 82668134 | 82667876 | ID=MsG0180004777.01.T01:three_prime_utr;Parent=MsG0180004777.01.T01 |
| Gene Sequence |
>MsG0180004777.01.T01 ATGGAGAAGAAAGAGATGGGTGTAAGTGTGAAGAGTGAGGATGTTATTGTTGATTCTTCCTCATCTTTGCTAAATGGTTATCAATTTTCAAGCGTGTTTGATTTCTGTGAGGTTGAAAGAAGTTCTTCTTTAGGGTTTATGGAGTTACTTGGTGGTCAGGACTATACTCCTCTCCTTGATGTACCAACCATGTCTGCAGTGACTCATAATCACAATACAGTTAAATCTTCATCTGAAAAAGAGTGTTCTGAGGTTTTGAACCAGCAACAACCTGCAACTCCTAACTCTTGTTCGATTTCATCTGCATCTAGTGAAGCAGTCAATGATAATAAGATTGTTGTTAACCAAGCAGAAGAAGATGAAGAAGAAGAAGAAAAGCAAAAGACAAACAAACAGTTGAAAACGAAGAAGACAAATCTAAAGAGACAGAGAGAACCAAGATTTGCTTTCATGACGAAAAGCGAGGTTGATCATCTGGAAGATGGGTACAGATGGAGAAAGTACGGTCAAAAAGCTGTCAAAAATAGCCCTTTTCCAAGGAGCTACTATCGTTGCACCACTGCTTCATGTAATGTCAAGAAACGTGTGGAACGTTCTTACACTGATCCAAGCATTGTAGTCACAACATATGAAGGCCAACACACACATCCAAGTCCAACTATGTCCCGTTCCGCCTTTGCCGGAGTTCAAATACCACCACCCGCCGGTGTAGTCTCGGGTGGATTTCCAGCTACTAACTTTGGTTCAGTTTTGCAAGGAAACTACTTATCACAATATCATCAACAACCCTATCAACATCAACAACTACTTGTTAATACATTGTCCTCTTTGAATCACCCTTATAACAATTCTTCATCATTCAAGAATTCACCTTTTACTGCCCAAGAGAGAGTACAGCTTTGTAATCAAGGGACAACTGCATTTCTTAGGGATAATGGGCTACTTCAGGATGTTGTTCCTTCACATATGTTGAAAGAAGAGTAG |
| Protein sequence |
>MsG0180004777.01.T01 MEKKEMGVSVKSEDVIVDSSSSLLNGYQFSSVFDFCEVERSSSLGFMELLGGQDYTPLLDVPTMSAVTHNHNTVKSSSEKECSEVLNQQQPATPNSCSISSASSEAVNDNKIVVNQAEEDEEEEEKQKTNKQLKTKKTNLKRQREPRFAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSYTDPSIVVTTYEGQHTHPSPTMSRSAFAGVQIPPPAGVVSGGFPATNFGSVLQGNYLSQYHQQPYQHQQLLVNTLSSLNHPYNNSSSFKNSPFTAQERVQLCNQGTTAFLRDNGLLQDVVPSHMLKEE* |
