Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041177.01.T01 | XP_013450194.1 | 94.146 | 205 | 12 | 0 | 1 | 205 | 1 | 205 | 2.78E-140 | 401 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041177.01.T01 | Q9SJ52 | 43.284 | 201 | 104 | 5 | 5 | 201 | 4 | 198 | 1.79E-55 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041177.01.T01 | A0A072U3C0 | 94.146 | 205 | 12 | 0 | 1 | 205 | 1 | 205 | 1.33e-140 | 401 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000234.01 | MsG0780041177.01 | 0.805780 | 1.131319e-49 | 6.038396e-47 |
| MsG0180003240.01 | MsG0780041177.01 | 0.808063 | 3.723673e-50 | 2.108135e-47 |
| MsG0180004328.01 | MsG0780041177.01 | 0.821886 | 3.180288e-53 | 2.620362e-50 |
| MsG0180005648.01 | MsG0780041177.01 | 0.814418 | 1.556483e-51 | 1.043869e-48 |
| MsG0780041176.01 | MsG0780041177.01 | 0.840672 | 7.551399e-58 | 1.083462e-54 |
| MsG0780041177.01 | MsG0780041574.01 | 0.825799 | 3.848183e-54 | 3.544029e-51 |
| MsG0780041177.01 | MsG0880045272.01 | 0.827020 | 1.969962e-54 | 1.878691e-51 |
| MsG0780041177.01 | MsG0880045818.01 | 0.815427 | 9.298304e-52 | 6.408807e-49 |
| MsG0480023349.01 | MsG0780041177.01 | 0.815780 | 7.755461e-52 | 5.397860e-49 |
| MsG0480023658.01 | MsG0780041177.01 | 0.805721 | 1.164174e-49 | 6.204489e-47 |
| MsG0480023921.01 | MsG0780041177.01 | 0.832601 | 8.612115e-56 | 9.672785e-53 |
| MsG0380015834.01 | MsG0780041177.01 | 0.814153 | 1.780759e-51 | 1.185666e-48 |
| MsG0380016020.01 | MsG0780041177.01 | 0.817016 | 4.103298e-52 | 2.954043e-49 |
| MsG0380016839.01 | MsG0780041177.01 | 0.802209 | 6.254758e-49 | 3.045909e-46 |
| MsG0380017859.01 | MsG0780041177.01 | 0.833723 | 4.527823e-56 | 5.258729e-53 |
| MsG0580028909.01 | MsG0780041177.01 | 0.803070 | 4.154466e-49 | 2.067954e-46 |
| MsG0580029626.01 | MsG0780041177.01 | 0.813461 | 2.530075e-51 | 1.653393e-48 |
| MsG0680030634.01 | MsG0780041177.01 | 0.815549 | 8.731441e-52 | 6.038313e-49 |
| MsG0280010521.01 | MsG0780041177.01 | 0.802532 | 5.364649e-49 | 2.634135e-46 |
| MsG0780040168.01 | MsG0780041177.01 | 0.810035 | 1.407867e-50 | 8.397756e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041177.01.T01 | MTR_7g106000 | 94.146 | 205 | 12 | 0 | 1 | 205 | 1 | 205 | 3.37e-144 | 401 |
| MsG0780041177.01.T01 | MTR_7g105970 | 79.512 | 205 | 42 | 0 | 1 | 205 | 1 | 205 | 3.52e-109 | 312 |
| MsG0780041177.01.T01 | MTR_7g106010 | 73.000 | 200 | 53 | 1 | 6 | 204 | 2 | 201 | 2.32e-105 | 303 |
| MsG0780041177.01.T01 | MTR_7g106030 | 71.078 | 204 | 57 | 2 | 4 | 205 | 3 | 206 | 2.18e-98 | 285 |
| MsG0780041177.01.T01 | MTR_1g057400 | 62.703 | 185 | 66 | 2 | 22 | 204 | 2 | 185 | 7.64e-79 | 235 |
| MsG0780041177.01.T01 | MTR_4g119428 | 36.486 | 222 | 116 | 6 | 1 | 205 | 2 | 215 | 9.07e-42 | 142 |
| MsG0780041177.01.T01 | MTR_1g057290 | 44.974 | 189 | 99 | 2 | 19 | 205 | 8 | 193 | 1.90e-40 | 137 |
| MsG0780041177.01.T01 | MTR_7g105870 | 42.995 | 207 | 109 | 5 | 4 | 205 | 2 | 204 | 1.35e-39 | 135 |
| MsG0780041177.01.T01 | MTR_1g057270 | 39.732 | 224 | 109 | 5 | 1 | 205 | 1 | 217 | 1.40e-36 | 128 |
| MsG0780041177.01.T01 | MTR_1g057300 | 53.535 | 99 | 45 | 1 | 32 | 130 | 11 | 108 | 5.44e-24 | 92.4 |
| MsG0780041177.01.T01 | MTR_6g471090 | 31.250 | 176 | 105 | 5 | 28 | 200 | 5 | 167 | 6.80e-22 | 89.4 |
| MsG0780041177.01.T01 | MTR_6g071410 | 29.651 | 172 | 110 | 6 | 36 | 200 | 2 | 169 | 8.30e-18 | 78.6 |
| MsG0780041177.01.T01 | MTR_6g070890 | 29.651 | 172 | 110 | 6 | 36 | 200 | 2 | 169 | 8.30e-18 | 78.6 |
| MsG0780041177.01.T01 | MTR_6g471080 | 31.429 | 175 | 100 | 6 | 31 | 200 | 5 | 164 | 2.46e-17 | 77.4 |
| MsG0780041177.01.T01 | MTR_6g070900 | 29.630 | 162 | 104 | 5 | 36 | 191 | 2 | 159 | 9.46e-16 | 73.2 |
| MsG0780041177.01.T01 | MTR_6g071400 | 29.630 | 162 | 104 | 5 | 36 | 191 | 2 | 159 | 9.46e-16 | 73.2 |
| MsG0780041177.01.T01 | MTR_7g106120 | 31.148 | 183 | 107 | 7 | 21 | 199 | 4 | 171 | 2.57e-13 | 66.2 |
| MsG0780041177.01.T01 | MTR_5g087760 | 25.253 | 198 | 131 | 6 | 18 | 204 | 41 | 232 | 2.32e-12 | 64.3 |
| MsG0780041177.01.T01 | MTR_2g011350 | 30.714 | 140 | 89 | 4 | 54 | 191 | 36 | 169 | 2.46e-12 | 63.5 |
| MsG0780041177.01.T01 | MTR_7g106100 | 35.849 | 106 | 63 | 2 | 32 | 137 | 67 | 167 | 1.39e-11 | 62.4 |
| MsG0780041177.01.T01 | MTR_2g087000 | 27.439 | 164 | 110 | 3 | 27 | 190 | 39 | 193 | 3.29e-11 | 60.8 |
| MsG0780041177.01.T01 | MTR_2g048270 | 33.043 | 115 | 71 | 3 | 84 | 196 | 7 | 117 | 9.35e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780041177.01.T01 | AT2G35980 | 43.284 | 201 | 104 | 5 | 5 | 201 | 4 | 198 | 1.83e-56 | 179 |
| MsG0780041177.01.T01 | AT3G11650 | 36.111 | 216 | 111 | 4 | 4 | 200 | 3 | 210 | 3.65e-43 | 145 |
| MsG0780041177.01.T01 | AT5G06320 | 35.023 | 217 | 121 | 4 | 1 | 205 | 1 | 209 | 3.58e-38 | 132 |
| MsG0780041177.01.T01 | AT2G35460 | 34.884 | 215 | 118 | 5 | 9 | 205 | 5 | 215 | 8.10e-38 | 131 |
| MsG0780041177.01.T01 | AT4G05220 | 22.632 | 190 | 135 | 3 | 20 | 201 | 16 | 201 | 3.60e-15 | 71.6 |
| MsG0780041177.01.T01 | AT1G32270 | 32.414 | 145 | 93 | 2 | 63 | 206 | 1 | 141 | 9.90e-14 | 69.3 |
| MsG0780041177.01.T01 | AT1G32270 | 32.414 | 145 | 93 | 2 | 63 | 206 | 1 | 141 | 1.54e-13 | 68.9 |
| MsG0780041177.01.T01 | AT5G53730 | 25.490 | 153 | 105 | 4 | 54 | 201 | 37 | 185 | 3.76e-11 | 60.5 |
Find 47 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAACTCAAAGGAACTTTA+AGG | 0.223136 | 7:+87672157 | None:intergenic |
| GCTGTCTCTTCAGTTTCTTC+TGG | 0.267585 | 7:-87672658 | MsG0780041177.01.T01:CDS |
| TGAGGCTTTACTTCAGGATT+AGG | 0.300660 | 7:-87672232 | MsG0780041177.01.T01:CDS |
| TGTGCTGGTGGTGGAATGGC+GGG | 0.331387 | 7:+87672719 | None:intergenic |
| TCTATGTGAGGCTTTACTTC+AGG | 0.355824 | 7:-87672238 | MsG0780041177.01.T01:CDS |
| TTTCTCAGGACAACATGTTT+TGG | 0.356714 | 7:-87672321 | MsG0780041177.01.T01:CDS |
| AGACTCGGGGATTTGATATC+TGG | 0.358540 | 7:-87672206 | MsG0780041177.01.T01:CDS |
| GGTTGCTGCTCTTCTTGTAT+TGG | 0.359850 | 7:+87672357 | None:intergenic |
| ATCGCACTCATAGTTCTTGC+TGG | 0.377894 | 7:-87672626 | MsG0780041177.01.T01:CDS |
| TTGCGAGGGCGAGGTTGTGC+TGG | 0.439367 | 7:+87672704 | None:intergenic |
| TTCTGCTTCGGTGGTTGCGA+GGG | 0.450941 | 7:+87672690 | None:intergenic |
| TCCTGAGAAAACAGCACTCA+TGG | 0.453273 | 7:+87672334 | None:intergenic |
| TTTAAGGTCACATTCACCTT+AGG | 0.456537 | 7:+87672173 | None:intergenic |
| GGGCGAGGTTGTGCTGGTGG+TGG | 0.461649 | 7:+87672710 | None:intergenic |
| AGGTTGTGCTGGTGGTGGAA+TGG | 0.471755 | 7:+87672715 | None:intergenic |
| CTTCTGCTTCGGTGGTTGCG+AGG | 0.479014 | 7:+87672689 | None:intergenic |
| AGCAACAACAACTTCTGCTT+CGG | 0.479078 | 7:+87672678 | None:intergenic |
| CTTCGGTGGTTGCGAGGGCG+AGG | 0.496928 | 7:+87672695 | None:intergenic |
| TAGTACGCACCGTTCAATTG+TGG | 0.499335 | 7:+87672746 | None:intergenic |
| CAATTGAACGGTGCGTACTA+CGG | 0.499960 | 7:-87672743 | MsG0780041177.01.T01:CDS |
| TTGTGCTGGTGGTGGAATGG+CGG | 0.506260 | 7:+87672718 | None:intergenic |
| AACAACAACTTCTGCTTCGG+TGG | 0.516162 | 7:+87672681 | None:intergenic |
| TCAGGATTAGGTTCAGACTC+GGG | 0.521074 | 7:-87672220 | MsG0780041177.01.T01:CDS |
| CAACATCTATTATGATAAAG+TGG | 0.524570 | 7:-87672450 | MsG0780041177.01.T01:CDS |
| TTCGTCGACATAGAACTTGA+AGG | 0.533993 | 7:+87672562 | None:intergenic |
| CGAGGGCGAGGTTGTGCTGG+TGG | 0.535016 | 7:+87672707 | None:intergenic |
| GACATAGAACTTGAAGGGGC+GGG | 0.537286 | 7:+87672568 | None:intergenic |
| CCTGAGAAAACAGCACTCAT+GGG | 0.540981 | 7:+87672335 | None:intergenic |
| TTCAGGATTAGGTTCAGACT+CGG | 0.548093 | 7:-87672221 | MsG0780041177.01.T01:CDS |
| ATCTGGTGATTACAAGCCTA+AGG | 0.550508 | 7:-87672189 | MsG0780041177.01.T01:CDS |
| TCGTCGACATAGAACTTGAA+GGG | 0.552525 | 7:+87672563 | None:intergenic |
| GAGCATTCTACGAAGGGTCG+AGG | 0.554221 | 7:-87672421 | MsG0780041177.01.T01:CDS |
| GTACAACGAGATAGAAGATA+AGG | 0.563903 | 7:+87672594 | None:intergenic |
| ACATAGAACTTGAAGGGGCG+GGG | 0.572104 | 7:+87672569 | None:intergenic |
| CAGGATTAGGTTCAGACTCG+GGG | 0.604096 | 7:-87672219 | MsG0780041177.01.T01:CDS |
| AACAAGCAACCACAATTGAA+CGG | 0.610539 | 7:-87672755 | MsG0780041177.01.T01:CDS |
| CAATACACTAAACTACAACA+TGG | 0.612954 | 7:-87672510 | MsG0780041177.01.T01:CDS |
| CGACATAGAACTTGAAGGGG+CGG | 0.619644 | 7:+87672567 | None:intergenic |
| GTGCTGGTGGTGGAATGGCG+GGG | 0.625212 | 7:+87672720 | None:intergenic |
| CTGAGAAAACAGCACTCATG+GGG | 0.650128 | 7:+87672336 | None:intergenic |
| CGTCGACATAGAACTTGAAG+GGG | 0.659465 | 7:+87672564 | None:intergenic |
| GAGGCACGAGCATTCTACGA+AGG | 0.666387 | 7:-87672428 | MsG0780041177.01.T01:CDS |
| AGGCACGAGCATTCTACGAA+GGG | 0.671696 | 7:-87672427 | MsG0780041177.01.T01:CDS |
| AAGTTTATGATATCTATGTG+AGG | 0.684871 | 7:-87672250 | MsG0780041177.01.T01:CDS |
| CATCTATTATGATAAAGTGG+AGG | 0.687150 | 7:-87672447 | MsG0780041177.01.T01:CDS |
| GTGTGTGATCACATCAACAT+TGG | 0.703529 | 7:+87672394 | None:intergenic |
| GATAGAAGATAAGGACTGCG+AGG | 0.714786 | 7:+87672603 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGTTTATGATATCTATGTG+AGG | - | Chr7:87672677-87672696 | MsG0780041177.01.T01:CDS | 25.0% |
| ! | CAACATCTATTATGATAAAG+TGG | - | Chr7:87672477-87672496 | MsG0780041177.01.T01:CDS | 25.0% |
| !!! | TAGATGTTGAGTTTTTTGTT+TGG | + | Chr7:87672466-87672485 | None:intergenic | 25.0% |
| CAATACACTAAACTACAACA+TGG | - | Chr7:87672417-87672436 | MsG0780041177.01.T01:CDS | 30.0% | |
| CATCTATTATGATAAAGTGG+AGG | - | Chr7:87672480-87672499 | MsG0780041177.01.T01:CDS | 30.0% | |
| AACAAGCAACCACAATTGAA+CGG | - | Chr7:87672172-87672191 | MsG0780041177.01.T01:CDS | 35.0% | |
| GTACAACGAGATAGAAGATA+AGG | + | Chr7:87672336-87672355 | None:intergenic | 35.0% | |
| ! | TTTAAGGTCACATTCACCTT+AGG | + | Chr7:87672757-87672776 | None:intergenic | 35.0% |
| ! | TTTCTCAGGACAACATGTTT+TGG | - | Chr7:87672606-87672625 | MsG0780041177.01.T01:CDS | 35.0% |
| AGCAACAACAACTTCTGCTT+CGG | + | Chr7:87672252-87672271 | None:intergenic | 40.0% | |
| ATCTGGTGATTACAAGCCTA+AGG | - | Chr7:87672738-87672757 | MsG0780041177.01.T01:CDS | 40.0% | |
| GTGTGTGATCACATCAACAT+TGG | + | Chr7:87672536-87672555 | None:intergenic | 40.0% | |
| TCGTCGACATAGAACTTGAA+GGG | + | Chr7:87672367-87672386 | None:intergenic | 40.0% | |
| TCTATGTGAGGCTTTACTTC+AGG | - | Chr7:87672689-87672708 | MsG0780041177.01.T01:CDS | 40.0% | |
| TGAGGCTTTACTTCAGGATT+AGG | - | Chr7:87672695-87672714 | MsG0780041177.01.T01:CDS | 40.0% | |
| TTCAGGATTAGGTTCAGACT+CGG | - | Chr7:87672706-87672725 | MsG0780041177.01.T01:CDS | 40.0% | |
| TTCGTCGACATAGAACTTGA+AGG | + | Chr7:87672368-87672387 | None:intergenic | 40.0% | |
| AACAACAACTTCTGCTTCGG+TGG | + | Chr7:87672249-87672268 | None:intergenic | 45.0% | |
| AGACTCGGGGATTTGATATC+TGG | - | Chr7:87672721-87672740 | MsG0780041177.01.T01:CDS | 45.0% | |
| ATCGCACTCATAGTTCTTGC+TGG | - | Chr7:87672301-87672320 | MsG0780041177.01.T01:CDS | 45.0% | |
| CAATTGAACGGTGCGTACTA+CGG | - | Chr7:87672184-87672203 | MsG0780041177.01.T01:CDS | 45.0% | |
| CCTGAGAAAACAGCACTCAT+GGG | + | Chr7:87672595-87672614 | None:intergenic | 45.0% | |
| CGTCGACATAGAACTTGAAG+GGG | + | Chr7:87672366-87672385 | None:intergenic | 45.0% | |
| CTGAGAAAACAGCACTCATG+GGG | + | Chr7:87672594-87672613 | None:intergenic | 45.0% | |
| GATAGAAGATAAGGACTGCG+AGG | + | Chr7:87672327-87672346 | None:intergenic | 45.0% | |
| GCTGTCTCTTCAGTTTCTTC+TGG | - | Chr7:87672269-87672288 | MsG0780041177.01.T01:CDS | 45.0% | |
| GGTTGCTGCTCTTCTTGTAT+TGG | + | Chr7:87672573-87672592 | None:intergenic | 45.0% | |
| TAGTACGCACCGTTCAATTG+TGG | + | Chr7:87672184-87672203 | None:intergenic | 45.0% | |
| TCAGGATTAGGTTCAGACTC+GGG | - | Chr7:87672707-87672726 | MsG0780041177.01.T01:CDS | 45.0% | |
| TCCTGAGAAAACAGCACTCA+TGG | + | Chr7:87672596-87672615 | None:intergenic | 45.0% | |
| ACATAGAACTTGAAGGGGCG+GGG | + | Chr7:87672361-87672380 | None:intergenic | 50.0% | |
| AGGCACGAGCATTCTACGAA+GGG | - | Chr7:87672500-87672519 | MsG0780041177.01.T01:CDS | 50.0% | |
| CAGGATTAGGTTCAGACTCG+GGG | - | Chr7:87672708-87672727 | MsG0780041177.01.T01:CDS | 50.0% | |
| CGACATAGAACTTGAAGGGG+CGG | + | Chr7:87672363-87672382 | None:intergenic | 50.0% | |
| GACATAGAACTTGAAGGGGC+GGG | + | Chr7:87672362-87672381 | None:intergenic | 50.0% | |
| !!! | CCCATGAGTGCTGTTTTCTC+AGG | - | Chr7:87672592-87672611 | MsG0780041177.01.T01:CDS | 50.0% |
| GAGCATTCTACGAAGGGTCG+AGG | - | Chr7:87672506-87672525 | MsG0780041177.01.T01:CDS | 55.0% | |
| GAGGCACGAGCATTCTACGA+AGG | - | Chr7:87672499-87672518 | MsG0780041177.01.T01:CDS | 55.0% | |
| TTCTGCTTCGGTGGTTGCGA+GGG | + | Chr7:87672240-87672259 | None:intergenic | 55.0% | |
| !! | AGGTTGTGCTGGTGGTGGAA+TGG | + | Chr7:87672215-87672234 | None:intergenic | 55.0% |
| !! | TTGTGCTGGTGGTGGAATGG+CGG | + | Chr7:87672212-87672231 | None:intergenic | 55.0% |
| CTTCTGCTTCGGTGGTTGCG+AGG | + | Chr7:87672241-87672260 | None:intergenic | 60.0% | |
| !! | TGTGCTGGTGGTGGAATGGC+GGG | + | Chr7:87672211-87672230 | None:intergenic | 60.0% |
| TTGCGAGGGCGAGGTTGTGC+TGG | + | Chr7:87672226-87672245 | None:intergenic | 65.0% | |
| !! | GTGCTGGTGGTGGAATGGCG+GGG | + | Chr7:87672210-87672229 | None:intergenic | 65.0% |
| CGAGGGCGAGGTTGTGCTGG+TGG | + | Chr7:87672223-87672242 | None:intergenic | 70.0% | |
| CTTCGGTGGTTGCGAGGGCG+AGG | + | Chr7:87672235-87672254 | None:intergenic | 70.0% | |
| ! | GGGCGAGGTTGTGCTGGTGG+TGG | + | Chr7:87672220-87672239 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 87672163 | 87672786 | 87672163 | ID=MsG0780041177.01;Name=MsG0780041177.01 |
| Chr7 | mRNA | 87672163 | 87672786 | 87672163 | ID=MsG0780041177.01.T01;Parent=MsG0780041177.01;Name=MsG0780041177.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|207 |
| Chr7 | exon | 87672163 | 87672786 | 87672163 | ID=MsG0780041177.01.T01:exon:20452;Parent=MsG0780041177.01.T01 |
| Chr7 | CDS | 87672163 | 87672786 | 87672163 | ID=MsG0780041177.01.T01:cds;Parent=MsG0780041177.01.T01 |
| Gene Sequence |
| Protein sequence |
