AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030638.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030638.01.T01 MTR_6g008480 91.265 332 29 0 1 332 1 332 0.0 623
MsG0680030638.01.T01 MTR_5g036410 36.455 299 153 6 42 329 53 325 7.99e-58 191
MsG0680030638.01.T01 MTR_8g005900 38.255 298 147 7 41 329 37 306 4.13e-55 184
MsG0680030638.01.T01 MTR_8g062770 34.870 347 172 9 6 329 10 325 2.50e-53 179
MsG0680030638.01.T01 MTR_5g012680 36.207 290 147 9 47 329 54 312 6.95e-51 172
MsG0680030638.01.T01 MTR_5g036080 31.507 292 152 10 46 329 67 318 2.25e-35 131
MsG0680030638.01.T01 MTR_5g036087 27.586 290 175 9 48 333 55 313 3.12e-23 99.0
MsG0680030638.01.T01 MTR_5g036083 27.389 314 155 10 3 308 1 249 2.48e-21 93.2
MsG0680030638.01.T01 MTR_5g036093 26.224 286 162 11 57 329 35 284 6.50e-20 89.0
MsG0680030638.01.T01 MTR_5g036270 25.974 308 146 11 53 308 3 280 4.88e-19 87.0
MsG0680030638.01.T01 MTR_5g036290 27.703 296 150 13 30 308 57 305 3.51e-18 84.7
MsG0680030638.01.T01 MTR_5g036360 25.776 322 163 13 39 329 63 339 1.75e-16 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030638.01.T01 AT1G59970 37.162 296 156 6 39 329 43 313 4.92e-60 196
MsG0680030638.01.T01 AT4G16640 36.232 345 177 8 1 329 1 318 8.07e-57 188
MsG0680030638.01.T01 AT1G24140 33.807 352 182 11 5 329 1 328 1.04e-53 181
MsG0680030638.01.T01 AT1G70170 34.694 343 172 9 12 329 8 323 2.25e-53 180
MsG0680030638.01.T01 AT2G45040 37.634 279 136 7 56 329 50 295 4.23e-47 162

Find 89 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCATCATGTTGAACATTT+AGG 0.265192 6:+6491683 None:intergenic
AATTAAGTTATGGGAAATAA+TGG 0.270384 6:-6489946 MsG0680030638.01.T01:three_prime_UTR
GGTCTTCATGCTGGATTGTT+GGG 0.280757 6:-6490216 MsG0680030638.01.T01:CDS
CAAAAGGGACAAACACTAAA+AGG 0.294871 6:-6491745 MsG0680030638.01.T01:CDS
AAGTGGAGCCTTCCATCTTC+TGG 0.306584 6:+6491193 None:intergenic
TTTCTAAAAGGAGTAATAAA+AGG 0.309742 6:+6491851 None:intergenic
TACTGTGTCCAAGCCCTAAA+AGG 0.314707 6:+6490109 None:intergenic
TCCACACTTAATTAAGTTAT+GGG 0.317637 6:-6489955 MsG0680030638.01.T01:three_prime_UTR
TCTCCTTACACCTGCCCAAT+AGG 0.318351 6:+6490062 None:intergenic
CACACACTCTAGTGCCAATA+TGG 0.318442 6:-6491905 MsG0680030638.01.T01:exon
TTGCCGTAGCCGTTGGTCTT+TGG 0.323120 6:+6490175 None:intergenic
AGGGATACATAGCTGCATTT+TGG 0.323892 6:+6490082 None:intergenic
ATGCAGCTATGTATCCCTAT+TGG 0.324355 6:-6490077 MsG0680030638.01.T01:CDS
TGACTATGTTTAGGCAAATC+AGG 0.324771 6:+6491517 None:intergenic
TACGGCAAAACAATTTGATT+TGG 0.328072 6:-6490160 MsG0680030638.01.T01:CDS
TTCCACACTTAATTAAGTTA+TGG 0.336005 6:-6489956 MsG0680030638.01.T01:three_prime_UTR
AACAAGATTAAGAGCCATAT+TGG 0.339209 6:+6491891 None:intergenic
ATCTGTGGCTTTGCATGAAA+TGG 0.342954 6:-6490136 MsG0680030638.01.T01:CDS
TCTTCCAAGACTCACCAAAA+TGG 0.357094 6:-6491456 MsG0680030638.01.T01:CDS
CTTTGTTTGTGACTATGTTT+AGG 0.357411 6:+6491508 None:intergenic
ACTTTGATGGAGACGAAAAC+TGG 0.368546 6:-6491174 MsG0680030638.01.T01:CDS
CTTAAGACTTGTGGGATTGT+TGG 0.378391 6:+6491150 None:intergenic
CAAAATGGAGAGACTCAAAA+AGG 0.380859 6:-6491441 MsG0680030638.01.T01:CDS
TGGGAAATGGAATTTCTAAA+AGG 0.381930 6:+6491839 None:intergenic
ATTTCACATTCACGGAAGTT+GGG 0.387217 6:-6491318 MsG0680030638.01.T01:CDS
AGGTCTTCATGCTGGATTGT+TGG 0.390597 6:-6490217 MsG0680030638.01.T01:CDS
CTCCTTACACCTGCCCAATA+GGG 0.395717 6:+6490063 None:intergenic
GATGGAAGGCTCCACTTTGA+TGG 0.398360 6:-6491187 MsG0680030638.01.T01:CDS
TACCATCACCTACATGTAAC+TGG 0.404024 6:-6491592 MsG0680030638.01.T01:CDS
AAGTTATCCTTAAGACTTGT+GGG 0.410453 6:+6491142 None:intergenic
AAGTGTTGATGTGTTGAGTA+TGG 0.416250 6:-6491395 MsG0680030638.01.T01:CDS
CAACAATCCCACAAGTCTTA+AGG 0.419763 6:-6491149 MsG0680030638.01.T01:CDS
GATTTCACATTCACGGAAGT+TGG 0.420328 6:-6491319 MsG0680030638.01.T01:CDS
AAACCTCAAAGGTGTTCAAA+AGG 0.423946 6:-6491761 MsG0680030638.01.T01:CDS
TGCAGCTATGTATCCCTATT+GGG 0.424514 6:-6490076 MsG0680030638.01.T01:CDS
ACATTTCCTGGCCCATCAAA+AGG 0.433183 6:+6491235 None:intergenic
GTATGAGCTAGAACATTTCC+TGG 0.435265 6:+6491223 None:intergenic
ATTATTTCAGGTCTTCATGC+TGG 0.444176 6:-6490225 MsG0680030638.01.T01:intron
ATCCCTATTGGGCAGGTGTA+AGG 0.446624 6:-6490065 MsG0680030638.01.T01:CDS
ATGGTTCTTGACTCAACAAT+GGG 0.446939 6:+6491820 None:intergenic
CTTGACTCAACAATGGGAAA+TGG 0.449294 6:+6491826 None:intergenic
TGATTCCATCCACATCATCT+TGG 0.462472 6:+6490031 None:intergenic
AGTTAGTGATTTCACATTCA+CGG 0.469287 6:-6491326 MsG0680030638.01.T01:CDS
ACAAGTCTTAAGGATAACTT+TGG 0.471399 6:-6491139 MsG0680030638.01.T01:intron
CATACTTTCCCTCCAGAAGA+TGG 0.473529 6:-6491205 MsG0680030638.01.T01:CDS
ACAATTTGATTTGGAATCTG+TGG 0.478019 6:-6490151 MsG0680030638.01.T01:CDS
GTGAAATCCGATCACTATAT+CGG 0.481999 6:+6491282 None:intergenic
CGTAGCCGTTGGTCTTTGGA+AGG 0.484074 6:+6490179 None:intergenic
GTATGTTTGAAAATCTTTGA+GGG 0.484231 6:+6491612 None:intergenic
AGTTTGGATGAATGAATCCT+TGG 0.493372 6:+6491789 None:intergenic
TCCCATAACTTAATTAAGTG+TGG 0.505325 6:+6489954 None:intergenic
GTCTTCATGCTGGATTGTTG+GGG 0.519679 6:-6490215 MsG0680030638.01.T01:CDS
AAGGAGTAATAAAAGGAAAG+AGG 0.519969 6:+6491858 None:intergenic
AACCTCAAAGGTGTTCAAAA+GGG 0.530859 6:-6491760 MsG0680030638.01.T01:CDS
GGTATGTTTGAAAATCTTTG+AGG 0.532790 6:+6491611 None:intergenic
AAAATGGTCATTTAGAGTAG+TGG 0.537551 6:+6491654 None:intergenic
GCTATGTATCCCTATTGGGC+AGG 0.543151 6:-6490072 MsG0680030638.01.T01:CDS
CAAACAAAGTGGTTTAGAGA+AGG 0.546779 6:-6491494 MsG0680030638.01.T01:CDS
TCTGTGGCTTTGCATGAAAT+GGG 0.554545 6:-6490135 MsG0680030638.01.T01:CDS
CATTTCCTGGCCCATCAAAA+GGG 0.556591 6:+6491236 None:intergenic
AGATGATGTGGATGGAATCA+TGG 0.557045 6:-6490028 MsG0680030638.01.T01:CDS
AAAGTTATCCTTAAGACTTG+TGG 0.558696 6:+6491141 None:intergenic
CAAACTCTTCAAAACCTCAA+AGG 0.558738 6:-6491772 MsG0680030638.01.T01:CDS
AAGAACCATAAAACCTTCCA+AGG 0.561044 6:-6491806 MsG0680030638.01.T01:CDS
CTAAACCAAGATGATGTGGA+TGG 0.563005 6:-6490036 MsG0680030638.01.T01:CDS
TGGATGAATGAATCCTTGGA+AGG 0.563353 6:+6491793 None:intergenic
TATGGTTCTTGACTCAACAA+TGG 0.565742 6:+6491819 None:intergenic
GACTATGTTTAGGCAAATCA+GGG 0.568078 6:+6491518 None:intergenic
TTTCACATTCACGGAAGTTG+GGG 0.569728 6:-6491317 MsG0680030638.01.T01:CDS
ATCTTGAGTCTTCCACGTTG+TGG 0.572692 6:-6491544 MsG0680030638.01.T01:CDS
CTTCCAAAGACCAACGGCTA+CGG 0.579541 6:-6490178 MsG0680030638.01.T01:CDS
AAATCTAAACCAAGATGATG+TGG 0.581039 6:-6490040 MsG0680030638.01.T01:CDS
TCATGCTGGATTGTTGGGGA+AGG 0.584729 6:-6490211 MsG0680030638.01.T01:CDS
TGGAGCCTTCCATCTTCTGG+AGG 0.591987 6:+6491196 None:intergenic
GGACAAACACTAAAAGGCAT+TGG 0.594183 6:-6491739 MsG0680030638.01.T01:CDS
GGATTTCACCATGGTGATCA+CGG 0.597300 6:-6491268 MsG0680030638.01.T01:CDS
TGGTCATTTAGAGTAGTGGA+AGG 0.597345 6:+6491658 None:intergenic
AAACATAGTCACAAACAAAG+TGG 0.601641 6:-6491505 MsG0680030638.01.T01:CDS
GTAGCCGTTGGTCTTTGGAA+GGG 0.606243 6:+6490180 None:intergenic
CTTTCCCTCCAGAAGATGGA+AGG 0.609700 6:-6491201 MsG0680030638.01.T01:CDS
AACTCGACCGATATAGTGAT+CGG 0.611162 6:-6491289 MsG0680030638.01.T01:CDS
GATCACTATATCGGTCGAGT+TGG 0.619534 6:+6491291 None:intergenic
GGAGCCTTCCATCTTCTGGA+GGG 0.638922 6:+6491197 None:intergenic
GACCAGTTACATGTAGGTGA+TGG 0.653297 6:+6491590 None:intergenic
TATTCCCTTCCAAAGACCAA+CGG 0.657183 6:-6490184 MsG0680030638.01.T01:CDS
AAATATCACCGTGATCACCA+TGG 0.688526 6:+6491260 None:intergenic
CTACACGACCAGTTACATGT+AGG 0.697312 6:+6491584 None:intergenic
ATAGTGATCGGATTTCACCA+TGG 0.731745 6:-6491277 MsG0680030638.01.T01:CDS
AAATCAGGGACACCACAACG+TGG 0.781696 6:+6491532 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTATTAAAATTATAA+AGG + Chr6:6490946-6490965 None:intergenic 0.0%
!! TTAAATTTGTATTATCATTA+TGG + Chr6:6491220-6491239 None:intergenic 10.0%
!!! AAATTTAATGTACAATTTTT+AGG - Chr6:6491232-6491251 MsG0680030638.01.T01:CDS 10.0%
!!! TATATTATATTTTTGTAAGT+TGG - Chr6:6491903-6491922 MsG0680030638.01.T01:exon 10.0%
!!! TATATTTTTAAAAAAAACTC+CGG - Chr6:6491414-6491433 MsG0680030638.01.T01:CDS 10.0%
!! AAAAATTACCTTAAAAGATT+TGG - Chr6:6490098-6490117 MsG0680030638.01.T01:CDS 15.0%
!! ATGAAGAAAAAAATTGTTAA+CGG - Chr6:6491440-6491459 MsG0680030638.01.T01:CDS 15.0%
!! ATTATGTATCAAAGTTTAAA+CGG - Chr6:6491154-6491173 MsG0680030638.01.T01:CDS 15.0%
!! CTTTATCATCTAATAAAAAA+AGG - Chr6:6490795-6490814 MsG0680030638.01.T01:intron 15.0%
!! AAGCACTAATTACTAATTAA+AGG + Chr6:6491491-6491510 None:intergenic 20.0%
!! AATTAAGTTATGGGAAATAA+TGG - Chr6:6491861-6491880 MsG0680030638.01.T01:CDS 20.0%
!! ACTACAACAAAAATTATTTC+AGG - Chr6:6491570-6491589 MsG0680030638.01.T01:CDS 20.0%
!!! AATTTTTAGGTTATCATGTT+AGG - Chr6:6491245-6491264 MsG0680030638.01.T01:CDS 20.0%
!!! ATTTTTAGGTTATCATGTTA+GGG - Chr6:6491246-6491265 MsG0680030638.01.T01:CDS 20.0%
!!! CAATTTTTTTCTTCATAAAC+CGG + Chr6:6491436-6491455 None:intergenic 20.0%
!!! TTTCTAAAAGGAGTAATAAA+AGG + Chr6:6489959-6489978 None:intergenic 20.0%
! GTATGTTTGAAAATCTTTGA+GGG + Chr6:6490198-6490217 None:intergenic 25.0%
! TACTAGCATGAGAAATATTA+AGG - Chr6:6491069-6491088 MsG0680030638.01.T01:intron 25.0%
! TCCACACTTAATTAAGTTAT+GGG - Chr6:6491852-6491871 MsG0680030638.01.T01:CDS 25.0%
! TTATAATATGAGATGAAAGG+AGG - Chr6:6491178-6491197 MsG0680030638.01.T01:CDS 25.0%
! TTCCACACTTAATTAAGTTA+TGG - Chr6:6491851-6491870 MsG0680030638.01.T01:CDS 25.0%
!! ATTCCAAGTTTTCATCAAAA+TGG + Chr6:6490172-6490191 None:intergenic 25.0%
AAACATAGTCACAAACAAAG+TGG - Chr6:6490302-6490321 MsG0680030638.01.T01:intron 30.0%
AAAGTTATCCTTAAGACTTG+TGG + Chr6:6490669-6490688 None:intergenic 30.0%
AAATCTAAACCAAGATGATG+TGG - Chr6:6491767-6491786 MsG0680030638.01.T01:CDS 30.0%
AACAAGATTAAGAGCCATAT+TGG + Chr6:6489919-6489938 None:intergenic 30.0%
AAGGAGTAATAAAAGGAAAG+AGG + Chr6:6489952-6489971 None:intergenic 30.0%
AAGTTATCCTTAAGACTTGT+GGG + Chr6:6490668-6490687 None:intergenic 30.0%
ACAAGTCTTAAGGATAACTT+TGG - Chr6:6490668-6490687 MsG0680030638.01.T01:intron 30.0%
AGTTAGTGATTTCACATTCA+CGG - Chr6:6490481-6490500 MsG0680030638.01.T01:intron 30.0%
GACAATGCATAAAATATGCA+AGG - Chr6:6491332-6491351 MsG0680030638.01.T01:CDS 30.0%
GGCTTATAATATGAGATGAA+AGG - Chr6:6491175-6491194 MsG0680030638.01.T01:CDS 30.0%
GGTATGTTTGAAAATCTTTG+AGG + Chr6:6490199-6490218 None:intergenic 30.0%
TACGGCAAAACAATTTGATT+TGG - Chr6:6491647-6491666 MsG0680030638.01.T01:CDS 30.0%
TAGCATCATGTTGAACATTT+AGG + Chr6:6490127-6490146 None:intergenic 30.0%
TCCCATAACTTAATTAAGTG+TGG + Chr6:6491856-6491875 None:intergenic 30.0%
TCTCTGCTTCATATTATAAG+TGG - Chr6:6490889-6490908 MsG0680030638.01.T01:intron 30.0%
TGGGAAATGGAATTTCTAAA+AGG + Chr6:6489971-6489990 None:intergenic 30.0%
! AAAATGGTCATTTAGAGTAG+TGG + Chr6:6490156-6490175 None:intergenic 30.0%
! CTTTGTTTGTGACTATGTTT+AGG + Chr6:6490302-6490321 None:intergenic 30.0%
! GTGATATTTACCCTTTTGAT+GGG - Chr6:6490561-6490580 MsG0680030638.01.T01:intron 30.0%
! TGGAAGACTCAAGATTTTTA+AGG + Chr6:6490258-6490277 None:intergenic 30.0%
! TTAGGTAGCCAAATCTTTTA+AGG + Chr6:6490109-6490128 None:intergenic 30.0%
!! ACAATTTGATTTGGAATCTG+TGG - Chr6:6491656-6491675 MsG0680030638.01.T01:CDS 30.0%
!! TGACCATTTTGATGAAAACT+TGG - Chr6:6490166-6490185 MsG0680030638.01.T01:CDS 30.0%
AAGAACCATAAAACCTTCCA+AGG - Chr6:6490001-6490020 MsG0680030638.01.T01:exon 35.0%
AGTTTGGATGAATGAATCCT+TGG + Chr6:6490021-6490040 None:intergenic 35.0%
ATGGTTCTTGACTCAACAAT+GGG + Chr6:6489990-6490009 None:intergenic 35.0%
ATTTCACATTCACGGAAGTT+GGG - Chr6:6490489-6490508 MsG0680030638.01.T01:intron 35.0%
CAAAAGGGACAAACACTAAA+AGG - Chr6:6490062-6490081 MsG0680030638.01.T01:CDS 35.0%
CAAAATGGAGAGACTCAAAA+AGG - Chr6:6490366-6490385 MsG0680030638.01.T01:intron 35.0%
CAAACAAAGTGGTTTAGAGA+AGG - Chr6:6490313-6490332 MsG0680030638.01.T01:intron 35.0%
CAAACTCTTCAAAACCTCAA+AGG - Chr6:6490035-6490054 MsG0680030638.01.T01:CDS 35.0%
GACTATGTTTAGGCAAATCA+GGG + Chr6:6490292-6490311 None:intergenic 35.0%
GTGAAATCCGATCACTATAT+CGG + Chr6:6490528-6490547 None:intergenic 35.0%
TAACGCTTGTATCAATCTAC+TGG - Chr6:6491279-6491298 MsG0680030638.01.T01:CDS 35.0%
TAGATTGATACAAGCGTTAG+AGG + Chr6:6491278-6491297 None:intergenic 35.0%
TATGGTTCTTGACTCAACAA+TGG + Chr6:6489991-6490010 None:intergenic 35.0%
TGACTATGTTTAGGCAAATC+AGG + Chr6:6490293-6490312 None:intergenic 35.0%
TGCATAAAATATGCAAGGTC+CGG - Chr6:6491337-6491356 MsG0680030638.01.T01:CDS 35.0%
! ATGAAATGGGTCACCTTTTA+GGG - Chr6:6491685-6491704 MsG0680030638.01.T01:CDS 35.0%
! ATTATTTCAGGTCTTCATGC+TGG - Chr6:6491582-6491601 MsG0680030638.01.T01:CDS 35.0%
! GGTGATATTTACCCTTTTGA+TGG - Chr6:6490560-6490579 MsG0680030638.01.T01:intron 35.0%
! TGAATCCTTGGAAGGTTTTA+TGG + Chr6:6490009-6490028 None:intergenic 35.0%
! TTACCACCAGTAGATTTTCT+TGG + Chr6:6491383-6491402 None:intergenic 35.0%
!! AAACCTCAAAGGTGTTCAAA+AGG - Chr6:6490046-6490065 MsG0680030638.01.T01:CDS 35.0%
!! AACCTCAAAGGTGTTCAAAA+GGG - Chr6:6490047-6490066 MsG0680030638.01.T01:CDS 35.0%
!! AAGTGTTGATGTGTTGAGTA+TGG - Chr6:6490412-6490431 MsG0680030638.01.T01:intron 35.0%
!!! CTTTGAGGTTTTGAAGAGTT+TGG + Chr6:6490037-6490056 None:intergenic 35.0%
!!! CTTTTTGAGTCTCTCCATTT+TGG + Chr6:6490368-6490387 None:intergenic 35.0%
AAATATCACCGTGATCACCA+TGG + Chr6:6490550-6490569 None:intergenic 40.0%
AACTCGACCGATATAGTGAT+CGG - Chr6:6490518-6490537 MsG0680030638.01.T01:intron 40.0%
ACTTTGATGGAGACGAAAAC+TGG - Chr6:6490633-6490652 MsG0680030638.01.T01:intron 40.0%
AGATGATGTGGATGGAATCA+TGG - Chr6:6491779-6491798 MsG0680030638.01.T01:CDS 40.0%
ATAGTGATCGGATTTCACCA+TGG - Chr6:6490530-6490549 MsG0680030638.01.T01:intron 40.0%
ATGCAGCTATGTATCCCTAT+TGG - Chr6:6491730-6491749 MsG0680030638.01.T01:CDS 40.0%
CAACAATCCCACAAGTCTTA+AGG - Chr6:6490658-6490677 MsG0680030638.01.T01:intron 40.0%
CATAAAATATGCAAGGTCCG+GGG - Chr6:6491339-6491358 MsG0680030638.01.T01:CDS 40.0%
CTAAACCAAGATGATGTGGA+TGG - Chr6:6491771-6491790 MsG0680030638.01.T01:CDS 40.0%
CTTAAGACTTGTGGGATTGT+TGG + Chr6:6490660-6490679 None:intergenic 40.0%
CTTGACTCAACAATGGGAAA+TGG + Chr6:6489984-6490003 None:intergenic 40.0%
GATTTCACATTCACGGAAGT+TGG - Chr6:6490488-6490507 MsG0680030638.01.T01:intron 40.0%
GCATAAAATATGCAAGGTCC+GGG - Chr6:6491338-6491357 MsG0680030638.01.T01:CDS 40.0%
GTATGAGCTAGAACATTTCC+TGG + Chr6:6490587-6490606 None:intergenic 40.0%
TACCATCACCTACATGTAAC+TGG - Chr6:6490215-6490234 MsG0680030638.01.T01:CDS 40.0%
TATTCCCTTCCAAAGACCAA+CGG - Chr6:6491623-6491642 MsG0680030638.01.T01:CDS 40.0%
TCTTCCAAGACTCACCAAAA+TGG - Chr6:6490351-6490370 MsG0680030638.01.T01:intron 40.0%
TGATTCCATCCACATCATCT+TGG + Chr6:6491779-6491798 None:intergenic 40.0%
TGCAGCTATGTATCCCTATT+GGG - Chr6:6491731-6491750 MsG0680030638.01.T01:CDS 40.0%
TGGATGAATGAATCCTTGGA+AGG + Chr6:6490017-6490036 None:intergenic 40.0%
TTTCACATTCACGGAAGTTG+GGG - Chr6:6490490-6490509 MsG0680030638.01.T01:intron 40.0%
! AGGGATACATAGCTGCATTT+TGG + Chr6:6491728-6491747 None:intergenic 40.0%
! AGTTTTCGTCTCCATCAAAG+TGG + Chr6:6490634-6490653 None:intergenic 40.0%
! ATCTGTGGCTTTGCATGAAA+TGG - Chr6:6491671-6491690 MsG0680030638.01.T01:CDS 40.0%
! CATGAAATGGGTCACCTTTT+AGG - Chr6:6491684-6491703 MsG0680030638.01.T01:CDS 40.0%
! GGACAAACACTAAAAGGCAT+TGG - Chr6:6490068-6490087 MsG0680030638.01.T01:CDS 40.0%
! TCTGTGGCTTTGCATGAAAT+GGG - Chr6:6491672-6491691 MsG0680030638.01.T01:CDS 40.0%
! TGGTCATTTAGAGTAGTGGA+AGG + Chr6:6490152-6490171 None:intergenic 40.0%
! TTTTGGTGAGTCTTGGAAGA+AGG + Chr6:6490351-6490370 None:intergenic 40.0%
!!! GTGAGAACGCGTTTTTAAGT+TGG - Chr6:6490453-6490472 MsG0680030638.01.T01:intron 40.0%
ACATTTCCTGGCCCATCAAA+AGG + Chr6:6490575-6490594 None:intergenic 45.0%
AGGTCTTCATGCTGGATTGT+TGG - Chr6:6491590-6491609 MsG0680030638.01.T01:CDS 45.0%
ATCTTGAGTCTTCCACGTTG+TGG - Chr6:6490263-6490282 MsG0680030638.01.T01:intron 45.0%
CATACTTTCCCTCCAGAAGA+TGG - Chr6:6490602-6490621 MsG0680030638.01.T01:intron 45.0%
CATTTCCTGGCCCATCAAAA+GGG + Chr6:6490574-6490593 None:intergenic 45.0%
CCACCAAGAAAATCTACTGG+TGG - Chr6:6491377-6491396 MsG0680030638.01.T01:CDS 45.0%
CCACCACCAAGAAAATCTAC+TGG - Chr6:6491374-6491393 MsG0680030638.01.T01:CDS 45.0%
CTACACGACCAGTTACATGT+AGG + Chr6:6490226-6490245 None:intergenic 45.0%
GACCAGTTACATGTAGGTGA+TGG + Chr6:6490220-6490239 None:intergenic 45.0%
GATCACTATATCGGTCGAGT+TGG + Chr6:6490519-6490538 None:intergenic 45.0%
GGATTTCACCATGGTGATCA+CGG - Chr6:6490539-6490558 MsG0680030638.01.T01:intron 45.0%
GGTCTTCATGCTGGATTGTT+GGG - Chr6:6491591-6491610 MsG0680030638.01.T01:CDS 45.0%
GTCTTCATGCTGGATTGTTG+GGG - Chr6:6491592-6491611 MsG0680030638.01.T01:CDS 45.0%
TACTGTGTCCAAGCCCTAAA+AGG + Chr6:6491701-6491720 None:intergenic 45.0%
! CCACCAGTAGATTTTCTTGG+TGG + Chr6:6491380-6491399 None:intergenic 45.0%
! CCAGTAGATTTTCTTGGTGG+TGG + Chr6:6491377-6491396 None:intergenic 45.0%
! GTCCCTTTTGAACACCTTTG+AGG + Chr6:6490052-6490071 None:intergenic 45.0%
! TGAGCTTAGCTCAGTTGGTA+TGG - Chr6:6491310-6491329 MsG0680030638.01.T01:CDS 45.0%
!! AATTGTTTTGCCGTAGCCGT+TGG + Chr6:6491642-6491661 None:intergenic 45.0%
!! ATTTACCCTTTTGATGGGCC+AGG - Chr6:6490566-6490585 MsG0680030638.01.T01:intron 45.0%
!! CTCTCCATTTTGGTGAGTCT+TGG + Chr6:6490358-6490377 None:intergenic 45.0%
AAATCAGGGACACCACAACG+TGG + Chr6:6490278-6490297 None:intergenic 50.0%
AAGTGGAGCCTTCCATCTTC+TGG + Chr6:6490617-6490636 None:intergenic 50.0%
ATCCCTATTGGGCAGGTGTA+AGG - Chr6:6491742-6491761 MsG0680030638.01.T01:CDS 50.0%
CATCGTGAGCTTAGCTCAGT+TGG - Chr6:6491305-6491324 MsG0680030638.01.T01:CDS 50.0%
CTCCTTACACCTGCCCAATA+GGG + Chr6:6491747-6491766 None:intergenic 50.0%
CTTCCAAAGACCAACGGCTA+CGG - Chr6:6491629-6491648 MsG0680030638.01.T01:CDS 50.0%
CTTTCCCTCCAGAAGATGGA+AGG - Chr6:6490606-6490625 MsG0680030638.01.T01:intron 50.0%
GATGGAAGGCTCCACTTTGA+TGG - Chr6:6490620-6490639 MsG0680030638.01.T01:intron 50.0%
GCTATGTATCCCTATTGGGC+AGG - Chr6:6491735-6491754 MsG0680030638.01.T01:CDS 50.0%
TCATGCTGGATTGTTGGGGA+AGG - Chr6:6491596-6491615 MsG0680030638.01.T01:CDS 50.0%
TCTCCTTACACCTGCCCAAT+AGG + Chr6:6491748-6491767 None:intergenic 50.0%
!! GTAGCCGTTGGTCTTTGGAA+GGG + Chr6:6491630-6491649 None:intergenic 50.0%
!!! ATGGGTCACCTTTTAGGGCT+TGG - Chr6:6491690-6491709 MsG0680030638.01.T01:CDS 50.0%
GGAGCCTTCCATCTTCTGGA+GGG + Chr6:6490613-6490632 None:intergenic 55.0%
TGGAGCCTTCCATCTTCTGG+AGG + Chr6:6490614-6490633 None:intergenic 55.0%
!! CGTAGCCGTTGGTCTTTGGA+AGG + Chr6:6491631-6491650 None:intergenic 55.0%
!! TTGCCGTAGCCGTTGGTCTT+TGG + Chr6:6491635-6491654 None:intergenic 55.0%
!!! GATTTTCTTGGTGGTGGCCG+AGG + Chr6:6491371-6491390 None:intergenic 55.0%
AAGGTCCGGGGTTCGAACCT+CGG - Chr6:6491351-6491370 MsG0680030638.01.T01:CDS 60.0%
GGTGGCCGAGGTTCGAACCC+CGG + Chr6:6491359-6491378 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr6 gene 6489903 6491926 6489903 ID=MsG0680030638.01;Name=MsG0680030638.01
Chr6 mRNA 6489903 6491926 6489903 ID=MsG0680030638.01.T01;Parent=MsG0680030638.01;Name=MsG0680030638.01.T01;_AED=0.49;_eAED=0.49;_QI=18|1|1|1|1|1|2|102|333
Chr6 exon 6489903 6490237 6489903 ID=MsG0680030638.01.T01:exon:6231;Parent=MsG0680030638.01.T01
Chr6 exon 6491140 6491926 6491140 ID=MsG0680030638.01.T01:exon:6230;Parent=MsG0680030638.01.T01
Chr6 five_prime_UTR 6491909 6491926 6491909 ID=MsG0680030638.01.T01:five_prime_utr;Parent=MsG0680030638.01.T01
Chr6 CDS 6491140 6491908 6491140 ID=MsG0680030638.01.T01:cds;Parent=MsG0680030638.01.T01
Chr6 CDS 6490005 6490237 6490005 ID=MsG0680030638.01.T01:cds;Parent=MsG0680030638.01.T01
Chr6 three_prime_UTR 6489903 6490004 6489903 ID=MsG0680030638.01.T01:three_prime_utr;Parent=MsG0680030638.01.T01
Gene Sequence

>MsG0680030638.01.T01

ATGGCTCTTAATCTTGTTAAACTTTCAACCTCTTTCCTTTTATTACTCCTTTTAGAAATTCCATTTCCCATTGTTGAGTCAAGAACCATAAAACCTTCCAAGGATTCATTCATCCAAACTCTTCAAAACCTCAAAGGTGTTCAAAAGGGACAAACACTAAAAGGCATTGGTGAACTCAAAAATTACCTTAAAAGATTTGGCTACCTAAATGTTCAACATGATGCTACTCCTTCCACTACTCTAAATGACCATTTTGATGAAAACTTGGAATTTGCCCTCAAAGATTTTCAAACATACCATCACCTACATGTAACTGGTCGTGTAGACACTACAACCTTAAAAATCTTGAGTCTTCCACGTTGTGGTGTCCCTGATTTGCCTAAACATAGTCACAAACAAAGTGGTTTAGAGAAGGCTTCAAGCTATACCTTCTTCCAAGACTCACCAAAATGGAGAGACTCAAAAAGGAATTTGAAATACATGTATAAATCAAGTGTTGATGTGTTGAGTATGGATGTTGTGAGAAAAGCGTGTGAGAACGCGTTTTTAAGTTGGTCAGAAGTTAGTGATTTCACATTCACGGAAGTTGGGGATGCCAACTCGACCGATATAGTGATCGGATTTCACCATGGTGATCACGGTGATATTTACCCTTTTGATGGGCCAGGAAATGTTCTAGCTCATACTTTCCCTCCAGAAGATGGAAGGCTCCACTTTGATGGAGACGAAAACTGGACCAACAATCCCACAAGTCTTAAGGATAACTTTGGTCTTCATGCTGGATTGTTGGGGAAGGAATCTATTCCCTTCCAAAGACCAACGGCTACGGCAAAACAATTTGATTTGGAATCTGTGGCTTTGCATGAAATGGGTCACCTTTTAGGGCTTGGACACAGTACTGACCAAAATGCAGCTATGTATCCCTATTGGGCAGGTGTAAGGAGAAATCTAAACCAAGATGATGTGGATGGAATCATGGCTCTTTATGTGCTTCATAGTTAA
Protein sequence

>MsG0680030638.01.T01

MALNLVKLSTSFLLLLLLEIPFPIVESRTIKPSKDSFIQTLQNLKGVQKGQTLKGIGELKNYLKRFGYLNVQHDATPSTTLNDHFDENLEFALKDFQTYHHLHVTGRVDTTTLKILSLPRCGVPDLPKHSHKQSGLEKASSYTFFQDSPKWRDSKRNLKYMYKSSVDVLSMDVVRKACENAFLSWSEVSDFTFTEVGDANSTDIVIGFHHGDHGDIYPFDGPGNVLAHTFPPEDGRLHFDGDENWTNNPTSLKDNFGLHAGLLGKESIPFQRPTATAKQFDLESVALHEMGHLLGLGHSTDQNAAMYPYWAGVRRNLNQDDVDGIMALYVLHS*