AlfalfaGEDB | MsG0680030691.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030691.01

KEGG: -
GO: -
PFAM: PF00314

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030691.01.T01	XP_003618434.1	94.815	270	14	0	15	284	1	270	0	530

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030691.01.T01	Q5DWG1	43.902	246	130	5	21	266	1	238	7.44E-69	216

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030691.01.T01	G7KK13	94.815	270	14	0	15	284	1	270	0.0	530

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0480024019.01	MsG0680030691.01	PPI
MsG0580024408.01	MsG0680030691.01	PPI
MsG0680030691.01	MsG0480020937.01	PPI
MsG0680030691.01	MsG0380017755.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030691.01.T01	MTR_6g009480	94.815	270	14	0	15	284	1	270	0.0	530
MsG0680030691.01.T01	MTR_6g079580	49.590	244	113	5	24	266	6	240	7.98e-78	237
MsG0680030691.01.T01	MTR_8g107140	47.511	221	109	4	47	266	31	245	2.11e-70	218
MsG0680030691.01.T01	MTR_3g068015	42.972	249	135	3	22	266	3	248	3.74e-69	215
MsG0680030691.01.T01	MTR_3g111620	44.583	240	128	4	27	266	12	246	3.50e-66	207
MsG0680030691.01.T01	MTR_8g036215	46.186	236	121	4	31	265	7	237	1.37e-65	206
MsG0680030691.01.T01	MTR_1g021945	48.899	227	106	7	45	268	28	247	7.44e-65	204
MsG0680030691.01.T01	MTR_5g059200	46.473	241	112	8	28	265	13	239	9.60e-64	202
MsG0680030691.01.T01	MTR_1g062340	44.538	238	122	7	31	266	13	242	3.87e-63	199
MsG0680030691.01.T01	MTR_5g022310	41.107	253	143	4	18	266	1	251	5.35e-63	199

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030691.01.T01	AT5G40020	64.961	254	86	2	20	273	5	255	5.87e-123	352
MsG0680030691.01.T01	AT5G24620	43.123	269	139	3	2	266	48	306	6.52e-70	224
MsG0680030691.01.T01	AT5G24620	43.123	269	139	3	2	266	69	327	8.00e-70	224
MsG0680030691.01.T01	AT1G18250	46.903	226	113	4	43	267	21	240	8.91e-70	216
MsG0680030691.01.T01	AT1G18250	46.903	226	113	4	43	267	22	241	9.29e-70	216
MsG0680030691.01.T01	AT5G24620	44.980	249	132	2	22	266	4	251	1.81e-69	221
MsG0680030691.01.T01	AT4G24180	44.402	259	132	5	15	266	3	256	2.43e-67	211
MsG0680030691.01.T01	AT1G73620	44.783	230	120	4	39	267	38	261	3.80e-67	210
MsG0680030691.01.T01	AT4G24180	44.402	259	132	5	15	266	3	256	4.89e-67	212
MsG0680030691.01.T01	AT4G24180	44.358	257	130	5	15	266	3	251	5.34e-67	210

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Find 66 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCATAGCTAAAATAATT+AGG	0.333353	6:-7273822	None:intergenic
AAATGTGATTATATATTCTA+AGG	0.337850	6:-7273877	None:intergenic
AAATTATGAAGAAAATTAAA+GGG	0.426233	6:-7272529	None:intergenic
AAATTCTAATGGAGAAGTTG+TGG	0.540338	6:+7273683	MsG0680030691.01.T01:CDS
AAATTCTAGCACCATGGTCA+TGG	0.538771	6:+7273352	MsG0680030691.01.T01:CDS
AAGGATCCACAACTCACAAG+TGG	0.632763	6:-7273858	None:intergenic
AATCCACCTTCAGCTATAAT+TGG	0.318536	6:-7273303	None:intergenic
AATTATGAAGAAAATTAAAG+GGG	0.640454	6:-7272528	None:intergenic
AATTCGAAGTGTAACATTCA+AGG	0.437934	6:+7273612	MsG0680030691.01.T01:CDS
ACACCAACAATTGAGGTTGA+CGG	0.605459	6:+7273957	MsG0680030691.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATTCTAATGGAGAAGTTG+TGG	+	Chr6:7273683-7273702	MsG0680030691.01.T01:CDS	30.0%
AATTCGAAGTGTAACATTCA+AGG	+	Chr6:7273612-7273631	MsG0680030691.01.T01:CDS	30.0%
ATAAATGTCCCTTTCCAATA+TGG	+	Chr6:7273253-7273272	MsG0680030691.01.T01:CDS	30.0%
CACTATAGATGCATATGAAA+TGG	-	Chr6:7272609-7272628	None:intergenic	30.0%
GAAGAAAATTAAAGGGGTTA+TGG	-	Chr6:7272525-7272544	None:intergenic	30.0%
GACAATGCATAATATATGCA+AGG	+	Chr6:7273059-7273078	MsG0680030691.01.T01:intron	30.0%
TAACATTCAAGGTTGTTGTA+AGG	+	Chr6:7273623-7273642	MsG0680030691.01.T01:CDS	30.0%
TGCATAATATATGCAAGGTT+CGG	+	Chr6:7273064-7273083	MsG0680030691.01.T01:intron	30.0%
TGGTTACTATTAGTAGTTAG+TGG	+	Chr6:7272740-7272759	MsG0680030691.01.T01:intron	30.0%
AATCCACCTTCAGCTATAAT+TGG	-	Chr6:7273306-7273325	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	7272464	7273995	7272464	ID=MsG0680030691.01;Name=MsG0680030691.01
Chr6	mRNA	7272464	7273995	7272464	ID=MsG0680030691.01.T01;Parent=MsG0680030691.01;Name=MsG0680030691.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|0\|0.5\|2\|0\|295
Chr6	exon	7272464	7272569	7272464	ID=MsG0680030691.01.T01:exon:6698;Parent=MsG0680030691.01.T01
Chr6	exon	7273214	7273995	7273214	ID=MsG0680030691.01.T01:exon:6699;Parent=MsG0680030691.01.T01
Chr6	CDS	7272464	7272569	7272464	ID=MsG0680030691.01.T01:cds;Parent=MsG0680030691.01.T01
Chr6	CDS	7273214	7273995	7273214	ID=MsG0680030691.01.T01:cds;Parent=MsG0680030691.01.T01

Gene Sequence

>MsG0680030691.01.T01

TGGTCATTGATCAACATATACCAAATTGTATTAAGATTCAGAATGCAGCTATTAGGGTCCATAACCCCTTTAATTTTCTTCATAATTTCGGCTTACATTTTCCATGGGCTTGTCGAAGGACATCAAGCAACATTCTATATACACAATAAATGTCCCTTTCCAATATGGCCAGCAACAGCACCAAACACTGGTCAACCAATTATAGCTGAAGGTGGATTCTACCTTCCTTCAGGCCAAACAAAGAAAATTCTAGCACCATGGTCATGGAGTGGTAGAATTTGGGCTAGAACAGGTTGTAATTTTGCTTCAAATAATTGGAAACCATCTTGTGAAACTGGGGATTGTGATGGAAGATTAGCTTGCAATGGACTCATTGGAACACCACCAGCTACACTTGTTGAAATCACACTTCAAGGTGATAAAGGGAAACCAAATTTCTATGATGTTAGTCTTGTTGATGGTTACAACATTCCGGTATCCGTCGTCGTCGCTAACAAGAACATAAATTCGAAGTGTAACATTCAAGGTTGTTGTAAGGATGTTAAAAGTTTGTGTCCTCGTGAGCTTGAAGTTTTAAATTCTAATGGAGAAGTTGTGGCTTGCAAAAGTGCTTGTTTGGCGTTTGATCTTGACAATTTTTGTTGTAGGAATGATTATGGAAGTCCTAAGAAGTGTAGGCCAAATGTTTACTCGAAAATATTTAAGGATGCTTGTCCTAATTATTTTAGCTATGCTTTTGACACTCCTACTCCACTTGTGAGTTGTGGATCCTTAGAATATATAATCACATTTTGTCCTTATGGATGGGGTGGTGCTGCTGGTGTGCCAACGGTTGAGGTTGACGGCGTAACACCAACAATTGAGGTTGACGGCGTATATGATGTCTGA

Protein sequence

>MsG0680030691.01.T01

WSLINIYQIVLRFRMQLLGSITPLIFFIISAYIFHGLVEGHQATFYIHNKCPFPIWPATAPNTGQPIIAEGGFYLPSGQTKKILAPWSWSGRIWARTGCNFASNNWKPSCETGDCDGRLACNGLIGTPPATLVEITLQGDKGKPNFYDVSLVDGYNIPVSVVVANKNINSKCNIQGCCKDVKSLCPRELEVLNSNGEVVACKSACLAFDLDNFCCRNDYGSPKKCRPNVYSKIFKDACPNYFSYAFDTPTPLVSCGSLEYIITFCPYGWGGAAGVPTVEVDGVTPTIEVDGVYDV*