Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030691.01.T01 | XP_003618434.1 | 94.815 | 270 | 14 | 0 | 15 | 284 | 1 | 270 | 0 | 530 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030691.01.T01 | Q5DWG1 | 43.902 | 246 | 130 | 5 | 21 | 266 | 1 | 238 | 7.44E-69 | 216 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030691.01.T01 | G7KK13 | 94.815 | 270 | 14 | 0 | 15 | 284 | 1 | 270 | 0.0 | 530 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680030691.01 | MsG0480020937.01 | PPI |
| MsG0680030691.01 | MsG0380017755.01 | PPI |
| MsG0580024408.01 | MsG0680030691.01 | PPI |
| MsG0480024019.01 | MsG0680030691.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030691.01.T01 | MTR_6g009480 | 94.815 | 270 | 14 | 0 | 15 | 284 | 1 | 270 | 0.0 | 530 |
| MsG0680030691.01.T01 | MTR_6g079580 | 49.590 | 244 | 113 | 5 | 24 | 266 | 6 | 240 | 7.98e-78 | 237 |
| MsG0680030691.01.T01 | MTR_8g107140 | 47.511 | 221 | 109 | 4 | 47 | 266 | 31 | 245 | 2.11e-70 | 218 |
| MsG0680030691.01.T01 | MTR_3g068015 | 42.972 | 249 | 135 | 3 | 22 | 266 | 3 | 248 | 3.74e-69 | 215 |
| MsG0680030691.01.T01 | MTR_3g111620 | 44.583 | 240 | 128 | 4 | 27 | 266 | 12 | 246 | 3.50e-66 | 207 |
| MsG0680030691.01.T01 | MTR_8g036215 | 46.186 | 236 | 121 | 4 | 31 | 265 | 7 | 237 | 1.37e-65 | 206 |
| MsG0680030691.01.T01 | MTR_1g021945 | 48.899 | 227 | 106 | 7 | 45 | 268 | 28 | 247 | 7.44e-65 | 204 |
| MsG0680030691.01.T01 | MTR_5g059200 | 46.473 | 241 | 112 | 8 | 28 | 265 | 13 | 239 | 9.60e-64 | 202 |
| MsG0680030691.01.T01 | MTR_1g062340 | 44.538 | 238 | 122 | 7 | 31 | 266 | 13 | 242 | 3.87e-63 | 199 |
| MsG0680030691.01.T01 | MTR_5g022310 | 41.107 | 253 | 143 | 4 | 18 | 266 | 1 | 251 | 5.35e-63 | 199 |
| MsG0680030691.01.T01 | MTR_1g062630 | 42.642 | 265 | 139 | 8 | 20 | 282 | 2 | 255 | 6.56e-63 | 199 |
| MsG0680030691.01.T01 | MTR_5g022310 | 41.107 | 253 | 143 | 4 | 18 | 266 | 1 | 251 | 1.13e-62 | 200 |
| MsG0680030691.01.T01 | MTR_3g114030 | 47.807 | 228 | 113 | 6 | 42 | 266 | 6 | 230 | 1.27e-62 | 198 |
| MsG0680030691.01.T01 | MTR_4g073720 | 43.254 | 252 | 129 | 8 | 15 | 265 | 1 | 239 | 1.36e-62 | 201 |
| MsG0680030691.01.T01 | MTR_1g062350 | 44.958 | 238 | 121 | 7 | 31 | 266 | 13 | 242 | 2.05e-62 | 197 |
| MsG0680030691.01.T01 | MTR_7g062610 | 42.915 | 247 | 135 | 4 | 20 | 266 | 2 | 242 | 2.65e-62 | 197 |
| MsG0680030691.01.T01 | MTR_1g062370 | 44.538 | 238 | 122 | 7 | 31 | 266 | 13 | 242 | 3.72e-62 | 197 |
| MsG0680030691.01.T01 | MTR_1g025420 | 44.934 | 227 | 119 | 6 | 42 | 265 | 22 | 245 | 3.90e-62 | 197 |
| MsG0680030691.01.T01 | MTR_1g062660 | 44.538 | 238 | 122 | 7 | 31 | 266 | 13 | 242 | 4.57e-62 | 197 |
| MsG0680030691.01.T01 | MTR_4g073730 | 45.344 | 247 | 128 | 6 | 24 | 266 | 5 | 248 | 8.76e-62 | 197 |
| MsG0680030691.01.T01 | MTR_1g025420 | 45.333 | 225 | 117 | 6 | 44 | 265 | 24 | 245 | 8.81e-62 | 199 |
| MsG0680030691.01.T01 | MTR_4g073730 | 45.344 | 247 | 128 | 6 | 24 | 266 | 5 | 248 | 2.24e-61 | 197 |
| MsG0680030691.01.T01 | MTR_1g062590 | 41.767 | 249 | 135 | 6 | 20 | 266 | 2 | 242 | 3.63e-61 | 194 |
| MsG0680030691.01.T01 | MTR_8g037890 | 42.000 | 250 | 141 | 4 | 18 | 266 | 1 | 247 | 5.95e-61 | 196 |
| MsG0680030691.01.T01 | MTR_8g075550 | 44.958 | 238 | 120 | 5 | 35 | 265 | 20 | 253 | 7.08e-61 | 196 |
| MsG0680030691.01.T01 | MTR_2g069660 | 45.082 | 244 | 127 | 6 | 25 | 266 | 5 | 243 | 1.31e-60 | 193 |
| MsG0680030691.01.T01 | MTR_5g022350 | 43.388 | 242 | 121 | 8 | 28 | 267 | 17 | 244 | 2.88e-60 | 194 |
| MsG0680030691.01.T01 | MTR_7g102380 | 41.296 | 247 | 135 | 7 | 22 | 266 | 3 | 241 | 1.13e-59 | 191 |
| MsG0680030691.01.T01 | MTR_8g056820 | 41.767 | 249 | 140 | 5 | 20 | 266 | 2 | 247 | 3.70e-58 | 187 |
| MsG0680030691.01.T01 | MTR_8g075510 | 44.898 | 245 | 118 | 9 | 24 | 266 | 15 | 244 | 7.14e-58 | 186 |
| MsG0680030691.01.T01 | MTR_2g068030 | 43.498 | 223 | 120 | 5 | 45 | 265 | 25 | 243 | 1.41e-57 | 186 |
| MsG0680030691.01.T01 | MTR_8g075510 | 44.672 | 244 | 118 | 9 | 24 | 265 | 15 | 243 | 1.18e-56 | 185 |
| MsG0680030691.01.T01 | MTR_4g063630 | 42.678 | 239 | 128 | 6 | 32 | 265 | 17 | 251 | 1.65e-55 | 180 |
| MsG0680030691.01.T01 | MTR_2g067980 | 40.000 | 250 | 142 | 5 | 21 | 266 | 2 | 247 | 2.24e-53 | 187 |
| MsG0680030691.01.T01 | MTR_2g068655 | 41.921 | 229 | 129 | 4 | 40 | 267 | 28 | 253 | 9.93e-53 | 175 |
| MsG0680030691.01.T01 | MTR_1g062690 | 39.496 | 238 | 110 | 7 | 31 | 266 | 13 | 218 | 5.16e-49 | 162 |
| MsG0680030691.01.T01 | MTR_5g010635 | 36.078 | 255 | 126 | 11 | 41 | 293 | 22 | 241 | 1.33e-39 | 139 |
| MsG0680030691.01.T01 | MTR_8g096900 | 33.840 | 263 | 145 | 10 | 7 | 266 | 12 | 248 | 2.19e-36 | 130 |
| MsG0680030691.01.T01 | MTR_2g067990 | 38.667 | 225 | 108 | 8 | 43 | 266 | 27 | 222 | 1.78e-35 | 135 |
| MsG0680030691.01.T01 | MTR_5g010640 | 33.846 | 260 | 140 | 11 | 15 | 266 | 1 | 236 | 2.29e-35 | 127 |
| MsG0680030691.01.T01 | MTR_2g067970 | 39.785 | 186 | 105 | 7 | 84 | 266 | 1 | 182 | 2.12e-33 | 129 |
| MsG0680030691.01.T01 | MTR_5g023850 | 34.167 | 240 | 115 | 8 | 32 | 266 | 15 | 216 | 2.18e-33 | 122 |
| MsG0680030691.01.T01 | MTR_8g089020 | 45.385 | 130 | 64 | 3 | 126 | 255 | 31 | 153 | 5.42e-31 | 114 |
| MsG0680030691.01.T01 | MTR_5g022350 | 37.755 | 196 | 87 | 7 | 5 | 176 | 38 | 222 | 1.01e-30 | 115 |
| MsG0680030691.01.T01 | MTR_2g063150 | 35.052 | 194 | 119 | 4 | 55 | 247 | 46 | 233 | 7.36e-30 | 114 |
| MsG0680030691.01.T01 | MTR_1g062390 | 30.800 | 250 | 148 | 9 | 24 | 266 | 7 | 238 | 5.90e-25 | 100 |
| MsG0680030691.01.T01 | MTR_8g096910 | 37.500 | 168 | 85 | 8 | 20 | 186 | 2 | 150 | 1.02e-22 | 92.8 |
| MsG0680030691.01.T01 | MTR_8g096920 | 39.073 | 151 | 75 | 7 | 37 | 186 | 13 | 147 | 1.52e-21 | 89.7 |
| MsG0680030691.01.T01 | MTR_7g076360 | 31.797 | 217 | 80 | 6 | 40 | 255 | 5 | 154 | 2.05e-20 | 86.7 |
| MsG0680030691.01.T01 | MTR_3g081550 | 42.708 | 96 | 52 | 3 | 128 | 222 | 16 | 109 | 5.28e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030691.01.T01 | AT5G40020 | 64.961 | 254 | 86 | 2 | 20 | 273 | 5 | 255 | 5.87e-123 | 352 |
| MsG0680030691.01.T01 | AT5G24620 | 43.123 | 269 | 139 | 3 | 2 | 266 | 48 | 306 | 6.52e-70 | 224 |
| MsG0680030691.01.T01 | AT5G24620 | 43.123 | 269 | 139 | 3 | 2 | 266 | 69 | 327 | 8.00e-70 | 224 |
| MsG0680030691.01.T01 | AT1G18250 | 46.903 | 226 | 113 | 4 | 43 | 267 | 21 | 240 | 8.91e-70 | 216 |
| MsG0680030691.01.T01 | AT1G18250 | 46.903 | 226 | 113 | 4 | 43 | 267 | 22 | 241 | 9.29e-70 | 216 |
| MsG0680030691.01.T01 | AT5G24620 | 44.980 | 249 | 132 | 2 | 22 | 266 | 4 | 251 | 1.81e-69 | 221 |
| MsG0680030691.01.T01 | AT4G24180 | 44.402 | 259 | 132 | 5 | 15 | 266 | 3 | 256 | 2.43e-67 | 211 |
| MsG0680030691.01.T01 | AT1G73620 | 44.783 | 230 | 120 | 4 | 39 | 267 | 38 | 261 | 3.80e-67 | 210 |
| MsG0680030691.01.T01 | AT4G24180 | 44.402 | 259 | 132 | 5 | 15 | 266 | 3 | 256 | 4.89e-67 | 212 |
| MsG0680030691.01.T01 | AT4G24180 | 44.358 | 257 | 130 | 5 | 15 | 266 | 3 | 251 | 5.34e-67 | 210 |
| MsG0680030691.01.T01 | AT4G24180 | 44.358 | 257 | 130 | 5 | 15 | 266 | 3 | 251 | 1.60e-66 | 210 |
| MsG0680030691.01.T01 | AT1G75050 | 42.798 | 243 | 130 | 7 | 25 | 266 | 9 | 243 | 4.63e-64 | 202 |
| MsG0680030691.01.T01 | AT4G38660 | 45.815 | 227 | 113 | 5 | 41 | 266 | 28 | 245 | 5.71e-64 | 205 |
| MsG0680030691.01.T01 | AT4G38660 | 45.455 | 231 | 116 | 5 | 37 | 266 | 2 | 223 | 6.70e-64 | 204 |
| MsG0680030691.01.T01 | AT1G77700 | 42.803 | 264 | 137 | 7 | 4 | 265 | 20 | 271 | 2.69e-63 | 202 |
| MsG0680030691.01.T01 | AT1G77700 | 42.803 | 264 | 137 | 7 | 4 | 265 | 20 | 271 | 4.93e-63 | 202 |
| MsG0680030691.01.T01 | AT1G75040 | 46.091 | 243 | 118 | 10 | 25 | 266 | 9 | 239 | 1.94e-62 | 197 |
| MsG0680030691.01.T01 | AT1G77700 | 48.416 | 221 | 102 | 6 | 45 | 265 | 91 | 299 | 6.35e-62 | 200 |
| MsG0680030691.01.T01 | AT1G75030 | 41.732 | 254 | 135 | 7 | 15 | 266 | 1 | 243 | 1.39e-61 | 196 |
| MsG0680030691.01.T01 | AT2G28790 | 45.909 | 220 | 112 | 5 | 49 | 265 | 58 | 273 | 5.08e-61 | 195 |
| MsG0680030691.01.T01 | AT2G28790 | 42.683 | 246 | 134 | 5 | 23 | 265 | 7 | 248 | 6.43e-61 | 194 |
| MsG0680030691.01.T01 | AT4G24180 | 44.444 | 243 | 123 | 5 | 15 | 250 | 3 | 240 | 1.87e-60 | 195 |
| MsG0680030691.01.T01 | AT1G75800 | 44.000 | 250 | 127 | 8 | 23 | 266 | 6 | 248 | 2.68e-60 | 195 |
| MsG0680030691.01.T01 | AT4G24180 | 44.398 | 241 | 121 | 5 | 15 | 250 | 3 | 235 | 4.19e-60 | 194 |
| MsG0680030691.01.T01 | AT4G36010 | 41.991 | 231 | 128 | 3 | 41 | 266 | 21 | 250 | 9.86e-59 | 190 |
| MsG0680030691.01.T01 | AT4G36010 | 41.991 | 231 | 128 | 3 | 41 | 266 | 21 | 250 | 9.86e-59 | 190 |
| MsG0680030691.01.T01 | AT2G17860 | 42.041 | 245 | 134 | 5 | 26 | 266 | 9 | 249 | 1.27e-58 | 188 |
| MsG0680030691.01.T01 | AT1G20030 | 45.175 | 228 | 117 | 6 | 44 | 266 | 4 | 228 | 1.58e-58 | 189 |
| MsG0680030691.01.T01 | AT1G20030 | 45.175 | 228 | 117 | 6 | 44 | 266 | 21 | 245 | 2.19e-58 | 189 |
| MsG0680030691.01.T01 | AT5G02140 | 42.972 | 249 | 120 | 7 | 24 | 266 | 9 | 241 | 2.79e-58 | 189 |
| MsG0680030691.01.T01 | AT4G38670 | 41.870 | 246 | 137 | 6 | 24 | 266 | 5 | 247 | 3.23e-58 | 188 |
| MsG0680030691.01.T01 | AT4G38670 | 41.870 | 246 | 137 | 6 | 24 | 266 | 5 | 247 | 3.23e-58 | 188 |
| MsG0680030691.01.T01 | AT5G02140 | 42.972 | 249 | 120 | 7 | 24 | 266 | 9 | 241 | 4.01e-58 | 187 |
| MsG0680030691.01.T01 | AT1G19320 | 43.426 | 251 | 128 | 8 | 20 | 265 | 5 | 246 | 7.26e-57 | 184 |
| MsG0680030691.01.T01 | AT4G38670 | 42.489 | 233 | 129 | 5 | 37 | 266 | 1 | 231 | 2.33e-56 | 183 |
| MsG0680030691.01.T01 | AT5G38280 | 38.113 | 265 | 154 | 7 | 24 | 284 | 9 | 267 | 4.63e-52 | 181 |
| MsG0680030691.01.T01 | AT5G38280 | 38.113 | 265 | 154 | 7 | 24 | 284 | 9 | 267 | 7.86e-52 | 180 |
| MsG0680030691.01.T01 | AT4G18253 | 35.772 | 246 | 143 | 7 | 23 | 266 | 6 | 238 | 7.69e-42 | 145 |
| MsG0680030691.01.T01 | AT4G11650 | 35.878 | 262 | 140 | 10 | 27 | 284 | 4 | 241 | 1.22e-41 | 144 |
| MsG0680030691.01.T01 | AT4G36000 | 46.622 | 148 | 69 | 4 | 119 | 266 | 1 | 138 | 6.20e-38 | 133 |
| MsG0680030691.01.T01 | AT4G36000 | 46.622 | 148 | 69 | 4 | 119 | 266 | 1 | 138 | 6.20e-38 | 133 |
| MsG0680030691.01.T01 | AT4G18250 | 36.576 | 257 | 147 | 9 | 13 | 266 | 29 | 272 | 4.88e-36 | 137 |
| MsG0680030691.01.T01 | AT1G70250 | 36.910 | 233 | 126 | 10 | 44 | 266 | 146 | 367 | 5.71e-33 | 128 |
| MsG0680030691.01.T01 | AT5G24620 | 47.500 | 120 | 59 | 1 | 151 | 266 | 15 | 134 | 1.20e-31 | 119 |
| MsG0680030691.01.T01 | AT2G24810 | 48.718 | 78 | 39 | 1 | 188 | 265 | 116 | 192 | 8.64e-17 | 77.4 |
Find 66 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTACTCGAAAATATTTA+AGG | 0.141708 | 6:+7273791 | MsG0680030691.01.T01:CDS |
| TTCTGAATCTTAATACAATT+TGG | 0.198968 | 6:-7272484 | None:intergenic |
| AGATTCAGAATGCAGCTATT+AGG | 0.225225 | 6:+7272497 | MsG0680030691.01.T01:CDS |
| AGACTAACATCATAGAAATT+TGG | 0.226393 | 6:-7273537 | None:intergenic |
| GGTCATGGAGTGGTAGAATT+TGG | 0.228734 | 6:+7273367 | MsG0680030691.01.T01:CDS |
| ACATTTGGCCTACACTTCTT+AGG | 0.242147 | 6:-7273771 | None:intergenic |
| CCAGTGTTTGGTGCTGTTGC+TGG | 0.252470 | 6:-7273276 | None:intergenic |
| TGGAAACCATCTTGTGAAAC+TGG | 0.283626 | 6:+7273423 | MsG0680030691.01.T01:CDS |
| ATCACACTTCAAGGTGATAA+AGG | 0.292993 | 6:+7273510 | MsG0680030691.01.T01:CDS |
| GATTCAGAATGCAGCTATTA+GGG | 0.308672 | 6:+7272498 | MsG0680030691.01.T01:CDS |
| GAAGAAAATTAAAGGGGTTA+TGG | 0.311977 | 6:-7272522 | None:intergenic |
| GAAATTATGAAGAAAATTAA+AGG | 0.312086 | 6:-7272530 | None:intergenic |
| AATCCACCTTCAGCTATAAT+TGG | 0.318536 | 6:-7273303 | None:intergenic |
| ATAAATGTCCCTTTCCAATA+TGG | 0.329767 | 6:+7273253 | MsG0680030691.01.T01:CDS |
| AAAGCATAGCTAAAATAATT+AGG | 0.333353 | 6:-7273822 | None:intergenic |
| AAATGTGATTATATATTCTA+AGG | 0.337850 | 6:-7273877 | None:intergenic |
| TATGATGTTAGTCTTGTTGA+TGG | 0.351124 | 6:+7273546 | MsG0680030691.01.T01:CDS |
| TATGGATGGGGTGGTGCTGC+TGG | 0.356773 | 6:+7273906 | MsG0680030691.01.T01:CDS |
| TGTTGATGGTTACAACATTC+CGG | 0.373965 | 6:+7273560 | MsG0680030691.01.T01:CDS |
| TTAGCTTGCAATGGACTCAT+TGG | 0.397789 | 6:+7273462 | MsG0680030691.01.T01:CDS |
| CTTGTTGAAATCACACTTCA+AGG | 0.399140 | 6:+7273501 | MsG0680030691.01.T01:CDS |
| GTCATGGAGTGGTAGAATTT+GGG | 0.407113 | 6:+7273368 | MsG0680030691.01.T01:CDS |
| GGTGGATTCTACCTTCCTTC+AGG | 0.409270 | 6:+7273318 | MsG0680030691.01.T01:CDS |
| GGCTTGCAAAAGTGCTTGTT+TGG | 0.422698 | 6:+7273704 | MsG0680030691.01.T01:CDS |
| AAATTATGAAGAAAATTAAA+GGG | 0.426233 | 6:-7272529 | None:intergenic |
| GGTAGAATTTGGGCTAGAAC+AGG | 0.432125 | 6:+7273378 | MsG0680030691.01.T01:CDS |
| AATTCGAAGTGTAACATTCA+AGG | 0.437934 | 6:+7273612 | MsG0680030691.01.T01:CDS |
| GGTGCTGTTGCTGGCCATAT+TGG | 0.448741 | 6:-7273267 | None:intergenic |
| CAATCCCCAGTTTCACAAGA+TGG | 0.451184 | 6:-7273429 | None:intergenic |
| TCACACTTCAAGGTGATAAA+GGG | 0.463282 | 6:+7273511 | MsG0680030691.01.T01:CDS |
| TAACATTCAAGGTTGTTGTA+AGG | 0.467243 | 6:+7273623 | MsG0680030691.01.T01:CDS |
| ATAATTGGTTGACCAGTGTT+TGG | 0.480494 | 6:-7273288 | None:intergenic |
| TTAGCGACGACGACGGATAC+CGG | 0.481979 | 6:-7273579 | None:intergenic |
| ACGCCGTCAACCTCAATTGT+TGG | 0.483971 | 6:-7273960 | None:intergenic |
| TGCTGGTGTGCCAACGGTTG+AGG | 0.503762 | 6:+7273923 | MsG0680030691.01.T01:CDS |
| TGGTGCTGCTGGTGTGCCAA+CGG | 0.504606 | 6:+7273917 | MsG0680030691.01.T01:CDS |
| CGGCGTAACACCAACAATTG+AGG | 0.509628 | 6:+7273950 | MsG0680030691.01.T01:CDS |
| GCAGCACCACCCCATCCATA+AGG | 0.512882 | 6:-7273903 | None:intergenic |
| GTGCCAACGGTTGAGGTTGA+CGG | 0.521033 | 6:+7273930 | MsG0680030691.01.T01:CDS |
| AAATTCTAGCACCATGGTCA+TGG | 0.538771 | 6:+7273352 | MsG0680030691.01.T01:CDS |
| AAATTCTAATGGAGAAGTTG+TGG | 0.540338 | 6:+7273683 | MsG0680030691.01.T01:CDS |
| CCTACTCCACTTGTGAGTTG+TGG | 0.542595 | 6:+7273852 | MsG0680030691.01.T01:CDS |
| GGAAACCATCTTGTGAAACT+GGG | 0.562657 | 6:+7273424 | MsG0680030691.01.T01:CDS |
| GTGATTTCAACAAGTGTAGC+TGG | 0.563029 | 6:-7273492 | None:intergenic |
| TCTTTGTTTGGCCTGAAGGA+AGG | 0.566171 | 6:-7273329 | None:intergenic |
| ATGGAAGTCCTAAGAAGTGT+AGG | 0.582085 | 6:+7273763 | MsG0680030691.01.T01:CDS |
| CCACAACTCACAAGTGGAGT+AGG | 0.582731 | 6:-7273852 | None:intergenic |
| GTTGCTGGCCATATTGGAAA+GGG | 0.593909 | 6:-7273261 | None:intergenic |
| GATGGAAGATTAGCTTGCAA+TGG | 0.600555 | 6:+7273453 | MsG0680030691.01.T01:CDS |
| ACACCAACAATTGAGGTTGA+CGG | 0.605459 | 6:+7273957 | MsG0680030691.01.T01:CDS |
| CAACCAATTATAGCTGAAGG+TGG | 0.611874 | 6:+7273300 | MsG0680030691.01.T01:CDS |
| GGTCAACCAATTATAGCTGA+AGG | 0.618100 | 6:+7273297 | MsG0680030691.01.T01:CDS |
| TGTTGCTGGCCATATTGGAA+AGG | 0.621427 | 6:-7273262 | None:intergenic |
| ACGCCGTCAACCTCAACCGT+TGG | 0.623078 | 6:-7273933 | None:intergenic |
| TGTGAAACTGGGGATTGTGA+TGG | 0.623588 | 6:+7273435 | MsG0680030691.01.T01:CDS |
| AAGGATCCACAACTCACAAG+TGG | 0.632763 | 6:-7273858 | None:intergenic |
| TTTAAAACTTCAAGCTCACG+AGG | 0.637599 | 6:-7273663 | None:intergenic |
| CAAAGAAAATTCTAGCACCA+TGG | 0.637826 | 6:+7273346 | MsG0680030691.01.T01:CDS |
| AATTATGAAGAAAATTAAAG+GGG | 0.640454 | 6:-7272528 | None:intergenic |
| TAATTTGTAGGGCTTGTCGA+AGG | 0.641451 | 6:+7273204 | MsG0680030691.01.T01:intron |
| GAAACCATCTTGTGAAACTG+GGG | 0.649627 | 6:+7273425 | MsG0680030691.01.T01:CDS |
| CCAGCAACAGCACCAAACAC+TGG | 0.657996 | 6:+7273276 | MsG0680030691.01.T01:CDS |
| GTTCTTGTTAGCGACGACGA+CGG | 0.658078 | 6:-7273586 | None:intergenic |
| CTAGCACCATGGTCATGGAG+TGG | 0.668399 | 6:+7273357 | MsG0680030691.01.T01:CDS |
| ATTCTACCACTCCATGACCA+TGG | 0.689426 | 6:-7273363 | None:intergenic |
| ATTTCAACAAGTGTAGCTGG+TGG | 0.757907 | 6:-7273489 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTATGAAGAAAATTAAA+GGG | - | Chr6:7272532-7272551 | None:intergenic | 10.0% |
| !!! | TTTAATTTTCTTCATAATTT+CGG | + | Chr6:7272532-7272551 | MsG0680030691.01.T01:CDS | 10.0% |
| !! | AAATGTGATTATATATTCTA+AGG | - | Chr6:7273880-7273899 | None:intergenic | 15.0% |
| !! | AATTATGAAGAAAATTAAAG+GGG | - | Chr6:7272531-7272550 | None:intergenic | 15.0% |
| !! | GAAATTATGAAGAAAATTAA+AGG | - | Chr6:7272533-7272552 | None:intergenic | 15.0% |
| !! | TTAAATATAACTACGTATAT+AGG | - | Chr6:7273139-7273158 | None:intergenic | 15.0% |
| !!! | ACTAAAACTAAATAATTTGT+AGG | + | Chr6:7273192-7273211 | MsG0680030691.01.T01:intron | 15.0% |
| !!! | CTAAAACTAAATAATTTGTA+GGG | + | Chr6:7273193-7273212 | MsG0680030691.01.T01:intron | 15.0% |
| !! | AAAGCATAGCTAAAATAATT+AGG | - | Chr6:7273825-7273844 | None:intergenic | 20.0% |
| !! | AAATCGCTTAAATGTTAAAT+CGG | - | Chr6:7272786-7272805 | None:intergenic | 20.0% |
| !! | CTATAAATATAGATTTACCA+TGG | - | Chr6:7272568-7272587 | None:intergenic | 20.0% |
| !! | TGTTTACTCGAAAATATTTA+AGG | + | Chr6:7273791-7273810 | MsG0680030691.01.T01:CDS | 20.0% |
| !! | TTCTGAATCTTAATACAATT+TGG | - | Chr6:7272487-7272506 | None:intergenic | 20.0% |
| !!! | AATATTTTCGAGTAAACATT+TGG | - | Chr6:7273789-7273808 | None:intergenic | 20.0% |
| !!! | CTCAAATTTTGATTTAGATT+GGG | + | Chr6:7272857-7272876 | MsG0680030691.01.T01:intron | 20.0% |
| !!! | CTTGAAGTTTTAAATTCTAA+TGG | + | Chr6:7273672-7273691 | MsG0680030691.01.T01:CDS | 20.0% |
| !!! | GTAATTTTGCTTCAAATAAT+TGG | + | Chr6:7273403-7273422 | MsG0680030691.01.T01:CDS | 20.0% |
| !!! | TAACATTTAAGCGATTTTAA+CGG | + | Chr6:7272789-7272808 | MsG0680030691.01.T01:intron | 20.0% |
| !!! | TCTCAAATTTTGATTTAGAT+TGG | + | Chr6:7272856-7272875 | MsG0680030691.01.T01:intron | 20.0% |
| ! | AGACTAACATCATAGAAATT+TGG | - | Chr6:7273540-7273559 | None:intergenic | 25.0% |
| ! | TGAAATGGATAGATATATAG+TGG | - | Chr6:7272594-7272613 | None:intergenic | 25.0% |
| !! | ATAATCACATTTTGTCCTTA+TGG | + | Chr6:7273888-7273907 | MsG0680030691.01.T01:CDS | 25.0% |
| !! | ATTTTCGCAATGAATCTTAT+TGG | + | Chr6:7272720-7272739 | MsG0680030691.01.T01:intron | 25.0% |
| !!! | ATCTTGACAATTTTTGTTGT+AGG | + | Chr6:7273733-7273752 | MsG0680030691.01.T01:CDS | 25.0% |
| !!! | TTTTGTTGTAGGAATGATTA+TGG | + | Chr6:7273744-7273763 | MsG0680030691.01.T01:CDS | 25.0% |
| AAATTCTAATGGAGAAGTTG+TGG | + | Chr6:7273683-7273702 | MsG0680030691.01.T01:CDS | 30.0% | |
| AATTCGAAGTGTAACATTCA+AGG | + | Chr6:7273612-7273631 | MsG0680030691.01.T01:CDS | 30.0% | |
| ATAAATGTCCCTTTCCAATA+TGG | + | Chr6:7273253-7273272 | MsG0680030691.01.T01:CDS | 30.0% | |
| CACTATAGATGCATATGAAA+TGG | - | Chr6:7272609-7272628 | None:intergenic | 30.0% | |
| GAAGAAAATTAAAGGGGTTA+TGG | - | Chr6:7272525-7272544 | None:intergenic | 30.0% | |
| GACAATGCATAATATATGCA+AGG | + | Chr6:7273059-7273078 | MsG0680030691.01.T01:intron | 30.0% | |
| TAACATTCAAGGTTGTTGTA+AGG | + | Chr6:7273623-7273642 | MsG0680030691.01.T01:CDS | 30.0% | |
| TGCATAATATATGCAAGGTT+CGG | + | Chr6:7273064-7273083 | MsG0680030691.01.T01:intron | 30.0% | |
| TGGTTACTATTAGTAGTTAG+TGG | + | Chr6:7272740-7272759 | MsG0680030691.01.T01:intron | 30.0% | |
| ! | GTGCTAGAATTTTCTTTGTT+TGG | - | Chr6:7273344-7273363 | None:intergenic | 30.0% |
| !! | TATGATGTTAGTCTTGTTGA+TGG | + | Chr6:7273546-7273565 | MsG0680030691.01.T01:CDS | 30.0% |
| AATCCACCTTCAGCTATAAT+TGG | - | Chr6:7273306-7273325 | None:intergenic | 35.0% | |
| AGATTCAGAATGCAGCTATT+AGG | + | Chr6:7272497-7272516 | MsG0680030691.01.T01:CDS | 35.0% | |
| ATAATTGGTTGACCAGTGTT+TGG | - | Chr6:7273291-7273310 | None:intergenic | 35.0% | |
| ATCACACTTCAAGGTGATAA+AGG | + | Chr6:7273510-7273529 | MsG0680030691.01.T01:CDS | 35.0% | |
| CAAAGAAAATTCTAGCACCA+TGG | + | Chr6:7273346-7273365 | MsG0680030691.01.T01:CDS | 35.0% | |
| CATAATATATGCAAGGTTCG+GGG | + | Chr6:7273066-7273085 | MsG0680030691.01.T01:intron | 35.0% | |
| CTTGTTGAAATCACACTTCA+AGG | + | Chr6:7273501-7273520 | MsG0680030691.01.T01:CDS | 35.0% | |
| GATTCAGAATGCAGCTATTA+GGG | + | Chr6:7272498-7272517 | MsG0680030691.01.T01:CDS | 35.0% | |
| GCATAATATATGCAAGGTTC+GGG | + | Chr6:7273065-7273084 | MsG0680030691.01.T01:intron | 35.0% | |
| TCACACTTCAAGGTGATAAA+GGG | + | Chr6:7273511-7273530 | MsG0680030691.01.T01:CDS | 35.0% | |
| ! | ACATTTTGTCCTTATGGATG+GGG | + | Chr6:7273894-7273913 | MsG0680030691.01.T01:CDS | 35.0% |
| ! | ATTTCGGCTTACATTTTCCA+TGG | + | Chr6:7272548-7272567 | MsG0680030691.01.T01:CDS | 35.0% |
| ! | CACATTTTGTCCTTATGGAT+GGG | + | Chr6:7273893-7273912 | MsG0680030691.01.T01:CDS | 35.0% |
| ! | TCACATTTTGTCCTTATGGA+TGG | + | Chr6:7273892-7273911 | MsG0680030691.01.T01:CDS | 35.0% |
| ! | TTTAAAACTTCAAGCTCACG+AGG | - | Chr6:7273666-7273685 | None:intergenic | 35.0% |
| !! | AGCACCGACACTAAAATTAA+AGG | + | Chr6:7272998-7273017 | MsG0680030691.01.T01:intron | 35.0% |
| !! | ATTTTCTTTGTTTGGCCTGA+AGG | - | Chr6:7273336-7273355 | None:intergenic | 35.0% |
| !! | CGTGCCTTTAATTTTAGTGT+CGG | - | Chr6:7273005-7273024 | None:intergenic | 35.0% |
| !! | TGTTGATGGTTACAACATTC+CGG | + | Chr6:7273560-7273579 | MsG0680030691.01.T01:CDS | 35.0% |
| !!! | AATGACGTGCCTTTTTTTTG+TGG | - | Chr6:7273107-7273126 | None:intergenic | 35.0% |
| AAATTCTAGCACCATGGTCA+TGG | + | Chr6:7273352-7273371 | MsG0680030691.01.T01:CDS | 40.0% | |
| ACACCAACAATTGAGGTTGA+CGG | + | Chr6:7273957-7273976 | MsG0680030691.01.T01:CDS | 40.0% | |
| ACATTTGGCCTACACTTCTT+AGG | - | Chr6:7273774-7273793 | None:intergenic | 40.0% | |
| ATTTCAACAAGTGTAGCTGG+TGG | - | Chr6:7273492-7273511 | None:intergenic | 40.0% | |
| CAACCAATTATAGCTGAAGG+TGG | + | Chr6:7273300-7273319 | MsG0680030691.01.T01:CDS | 40.0% | |
| GAAACCATCTTGTGAAACTG+GGG | + | Chr6:7273425-7273444 | MsG0680030691.01.T01:CDS | 40.0% | |
| GAGTTAAGCTTACGAGAACA+AGG | - | Chr6:7273029-7273048 | None:intergenic | 40.0% | |
| GGAAACCATCTTGTGAAACT+GGG | + | Chr6:7273424-7273443 | MsG0680030691.01.T01:CDS | 40.0% | |
| GGTCAACCAATTATAGCTGA+AGG | + | Chr6:7273297-7273316 | MsG0680030691.01.T01:CDS | 40.0% | |
| GTCATGGAGTGGTAGAATTT+GGG | + | Chr6:7273368-7273387 | MsG0680030691.01.T01:CDS | 40.0% | |
| GTGATTTCAACAAGTGTAGC+TGG | - | Chr6:7273495-7273514 | None:intergenic | 40.0% | |
| TCTCGTAAGCTTAACTCAAG+TGG | + | Chr6:7273032-7273051 | MsG0680030691.01.T01:intron | 40.0% | |
| TGGAAACCATCTTGTGAAAC+TGG | + | Chr6:7273423-7273442 | MsG0680030691.01.T01:CDS | 40.0% | |
| ! | ATGGAAGTCCTAAGAAGTGT+AGG | + | Chr6:7273763-7273782 | MsG0680030691.01.T01:CDS | 40.0% |
| ! | TAATTTGTAGGGCTTGTCGA+AGG | + | Chr6:7273204-7273223 | MsG0680030691.01.T01:intron | 40.0% |
| ! | TTAGTAGTTAGTGGACGCTA+AGG | + | Chr6:7272749-7272768 | MsG0680030691.01.T01:intron | 40.0% |
| !! | GATGGAAGATTAGCTTGCAA+TGG | + | Chr6:7273453-7273472 | MsG0680030691.01.T01:CDS | 40.0% |
| !! | TTAGCTTGCAATGGACTCAT+TGG | + | Chr6:7273462-7273481 | MsG0680030691.01.T01:CDS | 40.0% |
| AAGGATCCACAACTCACAAG+TGG | - | Chr6:7273861-7273880 | None:intergenic | 45.0% | |
| AAGGTTCGGGGTTTAAACCT+CGG | + | Chr6:7273078-7273097 | MsG0680030691.01.T01:intron | 45.0% | |
| ACGGCGTATATGATGTCTGA+TGG | + | Chr6:7273976-7273995 | MsG0680030691.01.T01:CDS | 45.0% | |
| AGTGGACGCTAAGGTTAATG+CGG | + | Chr6:7272758-7272777 | MsG0680030691.01.T01:intron | 45.0% | |
| ATTCTACCACTCCATGACCA+TGG | - | Chr6:7273366-7273385 | None:intergenic | 45.0% | |
| CAATCCCCAGTTTCACAAGA+TGG | - | Chr6:7273432-7273451 | None:intergenic | 45.0% | |
| CCTCGGTCACCACAAAAAAA+AGG | + | Chr6:7273095-7273114 | MsG0680030691.01.T01:intron | 45.0% | |
| GGTCATGGAGTGGTAGAATT+TGG | + | Chr6:7273367-7273386 | MsG0680030691.01.T01:CDS | 45.0% | |
| GTTGCTGGCCATATTGGAAA+GGG | - | Chr6:7273264-7273283 | None:intergenic | 45.0% | |
| TGTGAAACTGGGGATTGTGA+TGG | + | Chr6:7273435-7273454 | MsG0680030691.01.T01:CDS | 45.0% | |
| TGTTGCTGGCCATATTGGAA+AGG | - | Chr6:7273265-7273284 | None:intergenic | 45.0% | |
| ! | GGTAGAATTTGGGCTAGAAC+AGG | + | Chr6:7273378-7273397 | MsG0680030691.01.T01:CDS | 45.0% |
| ! | TCTTTGTTTGGCCTGAAGGA+AGG | - | Chr6:7273332-7273351 | None:intergenic | 45.0% |
| ! | TTTTGTCCTTATGGATGGGG+TGG | + | Chr6:7273897-7273916 | MsG0680030691.01.T01:CDS | 45.0% |
| !! | GGCTTGCAAAAGTGCTTGTT+TGG | + | Chr6:7273704-7273723 | MsG0680030691.01.T01:CDS | 45.0% |
| !!! | CCTTTTTTTTGTGGTGACCG+AGG | - | Chr6:7273098-7273117 | None:intergenic | 45.0% |
| ACGCCGTCAACCTCAATTGT+TGG | - | Chr6:7273963-7273982 | None:intergenic | 50.0% | |
| CCACAACTCACAAGTGGAGT+AGG | - | Chr6:7273855-7273874 | None:intergenic | 50.0% | |
| CCTACTCCACTTGTGAGTTG+TGG | + | Chr6:7273852-7273871 | MsG0680030691.01.T01:CDS | 50.0% | |
| CGGCGTAACACCAACAATTG+AGG | + | Chr6:7273950-7273969 | MsG0680030691.01.T01:CDS | 50.0% | |
| GTTCTTGTTAGCGACGACGA+CGG | - | Chr6:7273589-7273608 | None:intergenic | 50.0% | |
| ! | GGTGGATTCTACCTTCCTTC+AGG | + | Chr6:7273318-7273337 | MsG0680030691.01.T01:CDS | 50.0% |
| CCAGCAACAGCACCAAACAC+TGG | + | Chr6:7273276-7273295 | MsG0680030691.01.T01:CDS | 55.0% | |
| CTAGCACCATGGTCATGGAG+TGG | + | Chr6:7273357-7273376 | MsG0680030691.01.T01:CDS | 55.0% | |
| GTGCCAACGGTTGAGGTTGA+CGG | + | Chr6:7273930-7273949 | MsG0680030691.01.T01:CDS | 55.0% | |
| TTAGCGACGACGACGGATAC+CGG | - | Chr6:7273582-7273601 | None:intergenic | 55.0% | |
| !! | CCAGTGTTTGGTGCTGTTGC+TGG | - | Chr6:7273279-7273298 | None:intergenic | 55.0% |
| !! | GGTGCTGTTGCTGGCCATAT+TGG | - | Chr6:7273270-7273289 | None:intergenic | 55.0% |
| ACGCCGTCAACCTCAACCGT+TGG | - | Chr6:7273936-7273955 | None:intergenic | 60.0% | |
| GCAGCACCACCCCATCCATA+AGG | - | Chr6:7273906-7273925 | None:intergenic | 60.0% | |
| TGCTGGTGTGCCAACGGTTG+AGG | + | Chr6:7273923-7273942 | MsG0680030691.01.T01:CDS | 60.0% | |
| !! | TATGGATGGGGTGGTGCTGC+TGG | + | Chr6:7273906-7273925 | MsG0680030691.01.T01:CDS | 60.0% |
| !! | TGGTGCTGCTGGTGTGCCAA+CGG | + | Chr6:7273917-7273936 | MsG0680030691.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 7272464 | 7273995 | 7272464 | ID=MsG0680030691.01;Name=MsG0680030691.01 |
| Chr6 | mRNA | 7272464 | 7273995 | 7272464 | ID=MsG0680030691.01.T01;Parent=MsG0680030691.01;Name=MsG0680030691.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|0|0.5|2|0|295 |
| Chr6 | exon | 7272464 | 7272569 | 7272464 | ID=MsG0680030691.01.T01:exon:6698;Parent=MsG0680030691.01.T01 |
| Chr6 | exon | 7273214 | 7273995 | 7273214 | ID=MsG0680030691.01.T01:exon:6699;Parent=MsG0680030691.01.T01 |
| Chr6 | CDS | 7272464 | 7272569 | 7272464 | ID=MsG0680030691.01.T01:cds;Parent=MsG0680030691.01.T01 |
| Chr6 | CDS | 7273214 | 7273995 | 7273214 | ID=MsG0680030691.01.T01:cds;Parent=MsG0680030691.01.T01 |
| Gene Sequence |
| Protein sequence |
