AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680031053.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031053.01.T01 MTR_3g116380 96.887 257 7 1 1 257 52 307 0.0 517
MsG0680031053.01.T01 MTR_3g116410 54.911 224 100 1 30 252 52 275 2.55e-91 271
MsG0680031053.01.T01 MTR_4g069680 54.464 224 101 1 30 252 63 286 1.57e-89 267
MsG0680031053.01.T01 MTR_3g107830 51.852 243 111 3 16 252 13 255 7.01e-88 262
MsG0680031053.01.T01 MTR_4g069040 45.852 229 118 4 27 252 284 509 4.14e-67 216
MsG0680031053.01.T01 MTR_4g069570 43.636 220 118 4 37 253 120 336 9.53e-65 205
MsG0680031053.01.T01 MTR_3g116320 42.922 219 121 2 36 253 473 688 1.45e-62 208
MsG0680031053.01.T01 MTR_3g116270 40.639 219 126 2 36 253 291 506 1.83e-62 204
MsG0680031053.01.T01 MTR_4g069550 44.091 220 117 4 37 253 247 463 6.96e-61 199
MsG0680031053.01.T01 MTR_4g069520 43.182 220 119 4 37 253 507 723 1.56e-58 198
MsG0680031053.01.T01 MTR_4g069520 43.182 220 119 4 37 253 493 709 1.85e-58 197
MsG0680031053.01.T01 MTR_1g008500 42.793 222 121 4 32 251 166 383 2.01e-58 190
MsG0680031053.01.T01 MTR_3g109490 42.035 226 122 6 31 252 101 321 6.62e-54 177
MsG0680031053.01.T01 MTR_1g008530 41.589 214 118 5 41 251 13 222 6.75e-53 171
MsG0680031053.01.T01 MTR_1g008590 42.152 223 119 7 32 251 108 323 7.29e-53 174
MsG0680031053.01.T01 MTR_1g008560 41.667 228 124 6 29 251 105 328 1.04e-52 174
MsG0680031053.01.T01 MTR_0312s0030 41.704 223 120 7 32 251 108 323 2.70e-52 173
MsG0680031053.01.T01 MTR_1g007520 41.704 223 120 7 32 251 108 323 2.70e-52 173
MsG0680031053.01.T01 MTR_1g008510 41.364 220 120 5 34 251 112 324 5.45e-52 172
MsG0680031053.01.T01 MTR_1g008470 41.743 218 120 5 34 250 86 297 1.02e-51 171
MsG0680031053.01.T01 MTR_1g008450 39.035 228 129 5 24 250 92 310 2.76e-49 165
MsG0680031053.01.T01 MTR_6g086530 40.513 195 112 4 62 252 11 205 8.13e-49 160
MsG0680031053.01.T01 MTR_1g008460 40.826 218 119 5 34 250 86 294 1.39e-48 162
MsG0680031053.01.T01 MTR_1g008580 42.690 171 92 4 83 251 4 170 4.46e-43 144
MsG0680031053.01.T01 MTR_5g034320 31.984 247 157 4 6 251 391 627 1.30e-31 122
MsG0680031053.01.T01 MTR_8g064500 31.481 216 144 3 37 251 159 371 2.99e-30 116
MsG0680031053.01.T01 MTR_8g064530 32.407 216 140 4 37 250 429 640 2.52e-29 116
MsG0680031053.01.T01 MTR_8g064560 32.870 216 141 3 37 251 412 624 6.03e-29 115
MsG0680031053.01.T01 MTR_4g068420 37.857 140 65 4 117 253 1 121 1.62e-28 105
MsG0680031053.01.T01 MTR_3g078090 31.944 216 143 3 37 251 413 625 1.10e-27 111
MsG0680031053.01.T01 MTR_5g033350 31.633 196 131 2 56 250 5 198 1.58e-27 105
MsG0680031053.01.T01 MTR_5g033340 30.876 217 146 3 35 250 164 377 2.97e-27 108
MsG0680031053.01.T01 MTR_5g033340 30.968 155 105 1 80 234 1 153 1.24e-18 84.7
MsG0680031053.01.T01 MTR_5g033330 29.954 217 148 3 35 250 403 616 1.95e-24 102
MsG0680031053.01.T01 MTR_5g033330 30.846 201 135 3 35 234 195 392 3.42e-22 95.9
MsG0680031053.01.T01 MTR_5g033330 30.556 180 122 2 56 234 7 184 3.23e-20 90.1
MsG0680031053.01.T01 MTR_4g133840 30.303 198 117 5 56 250 7 186 3.15e-21 88.6
MsG0680031053.01.T01 MTR_8g445170 34.615 156 93 5 97 252 237 383 3.72e-21 92.0
MsG0680031053.01.T01 MTR_8g045890 39.286 140 76 5 113 252 301 431 5.52e-20 89.0
MsG0680031053.01.T01 MTR_8g045890 39.286 140 76 5 113 252 342 472 7.37e-20 88.6
MsG0680031053.01.T01 MTR_8g045890 39.286 140 76 5 113 252 379 509 9.08e-20 88.6
MsG0680031053.01.T01 MTR_8g445140 35.000 140 82 5 113 252 172 302 3.80e-19 85.1
MsG0680031053.01.T01 MTR_2g081610 34.043 141 84 5 112 252 128 259 4.69e-19 84.3
MsG0680031053.01.T01 MTR_8g045880 38.571 140 77 5 113 252 184 314 1.38e-18 84.0
MsG0680031053.01.T01 MTR_8g045880 38.571 140 77 5 113 252 219 349 3.05e-18 83.2
MsG0680031053.01.T01 MTR_8g045880 38.571 140 77 5 113 252 221 351 3.19e-18 83.2
MsG0680031053.01.T01 MTR_5g033360 27.189 217 136 4 35 250 25 220 5.12e-18 80.5
MsG0680031053.01.T01 MTR_8g046000 34.416 154 91 6 101 253 79 223 8.17e-18 80.5
MsG0680031053.01.T01 MTR_1g045860 32.653 147 91 5 106 252 121 259 1.40e-17 80.1
MsG0680031053.01.T01 MTR_2g081590 28.899 218 122 9 37 252 73 259 1.79e-16 77.4
MsG0680031053.01.T01 MTR_8g075930 38.824 85 49 2 159 241 4 87 6.42e-13 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680031053.01.T01 AT1G49320 48.458 227 111 3 31 251 52 278 1.67e-72 223
MsG0680031053.01.T01 AT5G25610 42.411 224 121 5 31 250 170 389 7.99e-56 184
MsG0680031053.01.T01 AT1G70370 33.333 219 136 5 37 251 411 623 5.53e-27 109
MsG0680031053.01.T01 AT1G70370 33.333 219 136 5 37 251 411 623 5.53e-27 109
MsG0680031053.01.T01 AT1G23760 32.579 221 135 6 37 251 407 619 8.50e-26 106
MsG0680031053.01.T01 AT1G60390 30.804 224 141 5 33 250 405 620 1.06e-24 103

Find 39 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCACATGGATACTTCTAAA+TGG 0.212561 6:-13375996 MsG0680031053.01.T01:CDS
TACTACAATTTCATACAAAA+TGG 0.266342 6:+13376059 None:intergenic
GACTTTGCAAATTACATGTT+TGG 0.295983 6:-13376255 MsG0680031053.01.T01:CDS
CCCTCCTGATAATCTGCTTT+GGG 0.308335 6:-13375905 MsG0680031053.01.T01:CDS
ATTTCAGTTCCAAATGCAAT+AGG 0.315492 6:-13376141 MsG0680031053.01.T01:CDS
AGCAGTTGATGAATCTTTCT+TGG 0.317340 6:+13376473 None:intergenic
TCCCTCCTGATAATCTGCTT+TGG 0.318544 6:-13375906 MsG0680031053.01.T01:CDS
GTTCCAAAGGATTTAAAAGT+TGG 0.342461 6:-13376519 MsG0680031053.01.T01:CDS
TTGTATGAAATTGTAGTAGA+AGG 0.362661 6:-13376054 MsG0680031053.01.T01:CDS
AAGTGTGTATTTCATGGCTT+TGG 0.373004 6:+13376347 None:intergenic
TTGTTCAAGTGTGTATTTCA+TGG 0.375445 6:+13376341 None:intergenic
GGTACCCAAAGCAGATTATC+AGG 0.389324 6:+13375901 None:intergenic
ATTGTAGTAGAAGGTGAAAA+TGG 0.391681 6:-13376045 MsG0680031053.01.T01:CDS
ACAAAATGGTATCACCCTTC+TGG 0.395540 6:+13376073 None:intergenic
AAATTGTGTAATTCTGTAAA+GGG 0.399598 6:+13376178 None:intergenic
TGACATACTGGAGAGTTTCC+AGG 0.407905 6:+13375934 None:intergenic
AAAGTCAAACAAAGACTCTA+AGG 0.413155 6:+13376272 None:intergenic
TGACAATAGAAAACTGCAAA+AGG 0.418592 6:+13376099 None:intergenic
GAAATTGTGTAATTCTGTAA+AGG 0.428701 6:+13376177 None:intergenic
GGGTGACACCCTATTGCATT+TGG 0.430364 6:+13376132 None:intergenic
AGATGGAGAAGAAATCTAGA+AGG 0.448342 6:+13376388 None:intergenic
TCACATGGATACTTCTAAAT+GGG 0.449148 6:-13375995 MsG0680031053.01.T01:CDS
TTTCAGTTCCAAATGCAATA+GGG 0.450082 6:-13376140 MsG0680031053.01.T01:CDS
TATTTCATGGCTTTGGCTTG+TGG 0.464005 6:+13376354 None:intergenic
GAGAAGAAATCTAGAAGGAA+AGG 0.485932 6:+13376393 None:intergenic
CGTGTGCTTAATGTAAAACC+TGG 0.488283 6:-13375952 MsG0680031053.01.T01:CDS
GGAGGGAAGAAATGACATAC+TGG 0.503691 6:+13375922 None:intergenic
CTGCAAAAGGGTAAGGCATA+GGG 0.513892 6:+13376112 None:intergenic
TAGAAAACTGCAAAAGGGTA+AGG 0.531191 6:+13376105 None:intergenic
GACAATAGAAAACTGCAAAA+GGG 0.537400 6:+13376100 None:intergenic
TATTATGGTGTTGCAGTCAA+AGG 0.559041 6:+13375880 None:intergenic
ACTGCAAAAGGGTAAGGCAT+AGG 0.564319 6:+13376111 None:intergenic
AGCTGCAGCAATTTGTCACA+TGG 0.567177 6:-13376010 MsG0680031053.01.T01:CDS
TTTCTATTGTCACAGCCAGA+AGG 0.572783 6:-13376088 MsG0680031053.01.T01:CDS
GTAATTCTGTAAAGGGAGAG+AGG 0.580516 6:+13376185 None:intergenic
TTCTATTGTCACAGCCAGAA+GGG 0.596892 6:-13376087 MsG0680031053.01.T01:CDS
GTAGTAGAAGGTGAAAATGG+AGG 0.623165 6:-13376042 MsG0680031053.01.T01:CDS
CCCAAAGCAGATTATCAGGA+GGG 0.626212 6:+13375905 None:intergenic
ACCCAAAGCAGATTATCAGG+AGG 0.663331 6:+13375904 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTGTGTAATTCTGTAAA+GGG + Chr6:13376340-13376359 None:intergenic 20.0%
!! TACTACAATTTCATACAAAA+TGG + Chr6:13376459-13376478 None:intergenic 20.0%
!! TCTTTCTTGGAAAAATAAAT+TGG + Chr6:13376032-13376051 None:intergenic 20.0%
!!! AAAACTTTGAATTTTGAGTT+GGG + Chr6:13376287-13376306 None:intergenic 20.0%
! ACAGAAAAAGATGATTTCAA+AGG - Chr6:13375929-13375948 MsG0680031053.01.T01:CDS 25.0%
! GAAATTGTGTAATTCTGTAA+AGG + Chr6:13376341-13376360 None:intergenic 25.0%
! TTGTATGAAATTGTAGTAGA+AGG - Chr6:13376461-13376480 MsG0680031053.01.T01:CDS 25.0%
!! TTTCCAACTTTTAAATCCTT+TGG + Chr6:13376002-13376021 None:intergenic 25.0%
!!! ATTTTGTTAGCTTCTTCTTT+TGG + Chr6:13376080-13376099 None:intergenic 25.0%
!!! CAAAACTTTGAATTTTGAGT+TGG + Chr6:13376288-13376307 None:intergenic 25.0%
AAAGTCAAACAAAGACTCTA+AGG + Chr6:13376246-13376265 None:intergenic 30.0%
ATTGTAGTAGAAGGTGAAAA+TGG - Chr6:13376470-13376489 MsG0680031053.01.T01:CDS 30.0%
ATTTCAGTTCCAAATGCAAT+AGG - Chr6:13376374-13376393 MsG0680031053.01.T01:CDS 30.0%
GACAATAGAAAACTGCAAAA+GGG + Chr6:13376418-13376437 None:intergenic 30.0%
GACTTTGCAAATTACATGTT+TGG - Chr6:13376260-13376279 MsG0680031053.01.T01:CDS 30.0%
GTTCCAAAGGATTTAAAAGT+TGG - Chr6:13375996-13376015 MsG0680031053.01.T01:CDS 30.0%
TCACATGGATACTTCTAAAT+GGG - Chr6:13376520-13376539 MsG0680031053.01.T01:CDS 30.0%
TGACAATAGAAAACTGCAAA+AGG + Chr6:13376419-13376438 None:intergenic 30.0%
TTGTTCAAGTGTGTATTTCA+TGG + Chr6:13376177-13376196 None:intergenic 30.0%
TTTCAGTTCCAAATGCAATA+GGG - Chr6:13376375-13376394 MsG0680031053.01.T01:CDS 30.0%
! ACAATGCAATTTTGAACCTA+TGG - Chr6:13376193-13376212 MsG0680031053.01.T01:CDS 30.0%
! TCTTCTTTTGGTAGGAATTT+TGG + Chr6:13376068-13376087 None:intergenic 30.0%
! TTTTACATTAAGCACACGAA+AGG + Chr6:13376561-13376580 None:intergenic 30.0%
!!! AGAAGGAAAGGTAGTTTTTT+TGG + Chr6:13376113-13376132 None:intergenic 30.0%
AAGTGTGTATTTCATGGCTT+TGG + Chr6:13376171-13376190 None:intergenic 35.0%
ACGAAAAACACGTTCAATTC+AGG + Chr6:13375978-13375997 None:intergenic 35.0%
AGATGGAGAAGAAATCTAGA+AGG + Chr6:13376130-13376149 None:intergenic 35.0%
CGAAAAACACGTTCAATTCA+GGG + Chr6:13375977-13375996 None:intergenic 35.0%
GAGAAGAAATCTAGAAGGAA+AGG + Chr6:13376125-13376144 None:intergenic 35.0%
GTCACATGGATACTTCTAAA+TGG - Chr6:13376519-13376538 MsG0680031053.01.T01:CDS 35.0%
TAGAAAACTGCAAAAGGGTA+AGG + Chr6:13376413-13376432 None:intergenic 35.0%
! AATTTTGTCTCTCCTTCCAT+AGG + Chr6:13376212-13376231 None:intergenic 35.0%
! TGCAATTTTGAACCTATGGA+AGG - Chr6:13376197-13376216 MsG0680031053.01.T01:CDS 35.0%
!! AGCAGTTGATGAATCTTTCT+TGG + Chr6:13376045-13376064 None:intergenic 35.0%
!! TGTTAGCTTCTTCTTTTGGT+AGG + Chr6:13376076-13376095 None:intergenic 35.0%
!!! GGTAGTTTTTTTGGTGTGAA+AGG + Chr6:13376104-13376123 None:intergenic 35.0%
!!! TTGTGGAGAGTTTTTTGAGA+TGG + Chr6:13376147-13376166 None:intergenic 35.0%
ACAAAATGGTATCACCCTTC+TGG + Chr6:13376445-13376464 None:intergenic 40.0%
CGTGTGCTTAATGTAAAACC+TGG - Chr6:13376563-13376582 MsG0680031053.01.T01:CDS 40.0%
GTAATTCTGTAAAGGGAGAG+AGG + Chr6:13376333-13376352 None:intergenic 40.0%
GTAGTAGAAGGTGAAAATGG+AGG - Chr6:13376473-13376492 MsG0680031053.01.T01:CDS 40.0%
TTCTATTGTCACAGCCAGAA+GGG - Chr6:13376428-13376447 MsG0680031053.01.T01:CDS 40.0%
TTTCTATTGTCACAGCCAGA+AGG - Chr6:13376427-13376446 MsG0680031053.01.T01:CDS 40.0%
! GAACGTGTTTTTCGTTCCAA+AGG - Chr6:13375983-13376002 MsG0680031053.01.T01:CDS 40.0%
! GAGAGAGGATTTTGTGACAT+GGG + Chr6:13376318-13376337 None:intergenic 40.0%
!! TATTTCATGGCTTTGGCTTG+TGG + Chr6:13376164-13376183 None:intergenic 40.0%
ACCCAAAGCAGATTATCAGG+AGG + Chr6:13376614-13376633 None:intergenic 45.0%
ACTGCAAAAGGGTAAGGCAT+AGG + Chr6:13376407-13376426 None:intergenic 45.0%
AGCTGCAGCAATTTGTCACA+TGG - Chr6:13376505-13376524 MsG0680031053.01.T01:CDS 45.0%
CCCAAAGCAGATTATCAGGA+GGG + Chr6:13376613-13376632 None:intergenic 45.0%
CCCTCCTGATAATCTGCTTT+GGG - Chr6:13376610-13376629 MsG0680031053.01.T01:CDS 45.0%
CTGCAAAAGGGTAAGGCATA+GGG + Chr6:13376406-13376425 None:intergenic 45.0%
GGAGGGAAGAAATGACATAC+TGG + Chr6:13376596-13376615 None:intergenic 45.0%
GGTACCCAAAGCAGATTATC+AGG + Chr6:13376617-13376636 None:intergenic 45.0%
TCCCTCCTGATAATCTGCTT+TGG - Chr6:13376609-13376628 MsG0680031053.01.T01:CDS 45.0%
TGACATACTGGAGAGTTTCC+AGG + Chr6:13376584-13376603 None:intergenic 45.0%
! GGAGAGAGGATTTTGTGACA+TGG + Chr6:13376319-13376338 None:intergenic 45.0%
!! GGATTTTGTGACATGGGTTG+TGG + Chr6:13376312-13376331 None:intergenic 45.0%
GGGTGACACCCTATTGCATT+TGG + Chr6:13376386-13376405 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr6 gene 13375882 13376655 13375882 ID=MsG0680031053.01;Name=MsG0680031053.01
Chr6 mRNA 13375882 13376655 13375882 ID=MsG0680031053.01.T01;Parent=MsG0680031053.01;Name=MsG0680031053.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|257
Chr6 exon 13375882 13376655 13375882 ID=MsG0680031053.01.T01:exon:9475;Parent=MsG0680031053.01.T01
Chr6 CDS 13375882 13376655 13375882 ID=MsG0680031053.01.T01:cds;Parent=MsG0680031053.01.T01
Gene Sequence

>MsG0680031053.01.T01

ATGGAAGTTGAAAACTCTGATCATGTAGAAATGAATAAAAAAGATGAACAGAAAAAGATGATTTCAAAGGATGTGCATCACAATCAAATAGACCCTGAATTGAACGTGTTTTTCGTTCCAAAGGATTTAAAAGTTGGAAAAATAATGCCAATTTATTTTTCCAAGAAAGATTCATCAACTGCTCCAAAATTCCTACCAAAAGAAGAAGCTAACAAAATTCCTTTCACACCAAAAAAACTACCTTTCCTTCTAGATTTCTTCTCCATCTCAAAAAACTCTCCACAAGCCAAAGCCATGAAATACACACTTGAACAATGCAATTTTGAACCTATGGAAGGAGAGACAAAATTCTGTGCTACATCCTTAGAGTCTTTGTTTGACTTTGCAAATTACATGTTTGGACCCAACTCAAAATTCAAAGTTTTGACCACAACCCATGTCACAAAATCCTCTCTCCCTTTACAGAATTACACAATTTCAAAAGTAAATGAAATTTCAGTTCCAAATGCAATAGGGTGTCACCCTATGCCTTACCCTTTTGCAGTTTTCTATTGTCACAGCCAGAAGGGTGATACCATTTTGTATGAAATTGTAGTAGAAGGTGAAAATGGAGGAATTGTTCAAGCTGCAGCAATTTGTCACATGGATACTTCTAAATGGGATGCAGATCATGTTGCCTTTCGTGTGCTTAATGTAAAACCTGGAAACTCTCCAGTATGTCATTTCTTCCCTCCTGATAATCTGCTTTGGGTACCTTTGACTGCAACACCATAA

Protein sequence

>MsG0680031053.01.T01

MEVENSDHVEMNKKDEQKKMISKDVHHNQIDPELNVFFVPKDLKVGKIMPIYFSKKDSSTAPKFLPKEEANKIPFTPKKLPFLLDFFSISKNSPQAKAMKYTLEQCNFEPMEGETKFCATSLESLFDFANYMFGPNSKFKVLTTTHVTKSSLPLQNYTISKVNEISVPNAIGCHPMPYPFAVFYCHSQKGDTILYEIVVEGENGGIVQAAAICHMDTSKWDADHVAFRVLNVKPGNSPVCHFFPPDNLLWVPLTATP*