Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042832.01.T01 | XP_003627446.1 | 94.03 | 134 | 7 | 1 | 1 | 134 | 1 | 133 | 1.31E-87 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042832.01.T01 | B7FMF3 | 94.030 | 134 | 7 | 1 | 1 | 134 | 1 | 133 | 6.24e-88 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048302.01 | MsG0880042832.01 | 0.808715 | 2.702497e-50 | 1.556345e-47 |
| MsG0180003792.01 | MsG0880042832.01 | -0.821847 | 3.248018e-53 | 2.673216e-50 |
| MsG0180003898.01 | MsG0880042832.01 | 0.835128 | 2.009118e-56 | 2.433878e-53 |
| MsG0180004506.01 | MsG0880042832.01 | -0.808340 | 3.250526e-50 | 1.853839e-47 |
| MsG0780040654.01 | MsG0880042832.01 | 0.818754 | 1.662163e-52 | 1.255075e-49 |
| MsG0880042832.01 | MsG0880043262.01 | 0.830222 | 3.316945e-55 | 3.473103e-52 |
| MsG0880042832.01 | MsG0880043537.01 | 0.829247 | 5.726290e-55 | 5.828656e-52 |
| MsG0880042832.01 | MsG0880044855.01 | 0.811096 | 8.306112e-51 | 5.095374e-48 |
| MsG0880042832.01 | MsG0880045256.01 | 0.830961 | 2.186408e-55 | 2.339338e-52 |
| MsG0880042832.01 | MsG0880045861.01 | 0.833779 | 4.382969e-56 | 5.099552e-53 |
| MsG0880042832.01 | MsG0880046615.01 | 0.801333 | 9.461323e-49 | 4.506408e-46 |
| MsG0880042832.01 | MsG0880046753.01 | 0.881175 | 2.984808e-70 | 1.730858e-66 |
| MsG0880042832.01 | MsG0880047142.01 | 0.809002 | 2.346294e-50 | 1.361578e-47 |
| MsG0280010824.01 | MsG0880042832.01 | 0.820246 | 7.588896e-53 | 5.972859e-50 |
| MsG0380012008.01 | MsG0880042832.01 | 0.817578 | 3.066580e-52 | 2.241773e-49 |
| MsG0380013100.01 | MsG0880042832.01 | 0.815408 | 9.388710e-52 | 6.467623e-49 |
| MsG0380013185.01 | MsG0880042832.01 | 0.816262 | 6.053258e-52 | 4.268921e-49 |
| MsG0680031053.01 | MsG0880042832.01 | 0.803648 | 3.152923e-49 | 1.592976e-46 |
| MsG0480021001.01 | MsG0880042832.01 | 0.829085 | 6.271689e-55 | 6.352285e-52 |
| MsG0480021166.01 | MsG0880042832.01 | -0.801986 | 6.949290e-49 | 3.365057e-46 |
| MsG0480022530.01 | MsG0880042832.01 | 0.830785 | 2.414908e-55 | 2.570438e-52 |
| MsG0480023318.01 | MsG0880042832.01 | 0.826297 | 2.931342e-54 | 2.737982e-51 |
| MsG0580024467.01 | MsG0880042832.01 | 0.840830 | 6.865656e-58 | 9.899554e-55 |
| MsG0580024812.01 | MsG0880042832.01 | 0.821386 | 4.150923e-53 | 3.372112e-50 |
| MsG0380015796.01 | MsG0880042832.01 | 0.812324 | 4.489566e-51 | 2.845854e-48 |
| MsG0380016702.01 | MsG0880042832.01 | 0.830362 | 3.064951e-55 | 3.222183e-52 |
| MsG0380018026.01 | MsG0880042832.01 | 0.813404 | 2.604473e-51 | 1.699305e-48 |
| MsG0280007868.01 | MsG0880042832.01 | 0.803031 | 4.233160e-49 | 2.105103e-46 |
| MsG0280008116.01 | MsG0880042832.01 | 0.814684 | 1.359007e-51 | 9.179523e-49 |
| MsG0280008856.01 | MsG0880042832.01 | 0.822694 | 2.065284e-53 | 1.741330e-50 |
| MsG0680035842.01 | MsG0880042832.01 | 0.805182 | 1.510489e-49 | 7.938849e-47 |
| MsG0780038857.01 | MsG0880042832.01 | 0.805522 | 1.282113e-49 | 6.797396e-47 |
| MsG0780040060.01 | MsG0880042832.01 | 0.825340 | 4.943309e-54 | 4.493312e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042832.01.T01 | MTR_8g023100 | 94.030 | 134 | 7 | 1 | 1 | 134 | 1 | 133 | 1.58e-91 | 261 |
| MsG0880042832.01.T01 | MTR_7g117550 | 53.488 | 129 | 45 | 6 | 1 | 123 | 1 | 120 | 2.18e-35 | 120 |
| MsG0880042832.01.T01 | MTR_7g117550 | 55.670 | 97 | 39 | 3 | 1 | 97 | 23 | 115 | 1.71e-29 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880042832.01.T01 | AT3G14280 | 46.250 | 80 | 36 | 2 | 49 | 128 | 48 | 120 | 6.33e-15 | 67.0 |
Find 30 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATTTGAATTGCAATAAAA+TGG | 0.221285 | 8:-14647188 | MsG0880042832.01.T01:exon |
| CACTCATAGCTTTGAGTTTG+AGG | 0.236813 | 8:-14647129 | MsG0880042832.01.T01:three_prime_UTR |
| TTCTTGAAGAATTAGCTTAA+TGG | 0.238281 | 8:-14648224 | MsG0880042832.01.T01:exon |
| GAATTATCATATTTCTGAAT+TGG | 0.275390 | 8:+14648172 | None:intergenic |
| AATTATCATATTTCTGAATT+GGG | 0.345181 | 8:+14648173 | None:intergenic |
| GGAAGAAGATGAGATAAATA+AGG | 0.373874 | 8:+14648274 | None:intergenic |
| AGTTGGTTTCAAATTGCAAA+AGG | 0.405294 | 8:-14647258 | MsG0880042832.01.T01:CDS |
| CTTTGGAGCATAGCATCGTT+TGG | 0.411384 | 8:+14648117 | None:intergenic |
| TGAATCTTCTAAAACAAAGT+TGG | 0.441522 | 8:-14647275 | MsG0880042832.01.T01:CDS |
| TCAAATAGCACATGTGTAGT+AGG | 0.455657 | 8:-14647060 | MsG0880042832.01.T01:three_prime_UTR |
| CTTGAAATTTGTCTATAAGA+TGG | 0.456782 | 8:+14647597 | None:intergenic |
| AGAGGTGGAAGAAGAAGATG+TGG | 0.466622 | 8:+14648253 | None:intergenic |
| GTTGGTTTCAAATTGCAAAA+GGG | 0.500062 | 8:-14647257 | MsG0880042832.01.T01:CDS |
| ATTTGTGAAGGGTATGATGA+AGG | 0.501766 | 8:-14647621 | MsG0880042832.01.T01:CDS |
| GACCAACGGTTCATCACAAA+AGG | 0.504204 | 8:-14647575 | MsG0880042832.01.T01:intron |
| GAAATTGATGATGCAATTGA+AGG | 0.528412 | 8:-14647231 | MsG0880042832.01.T01:CDS |
| GTGGAGAAATAATTTGTGAA+GGG | 0.550320 | 8:-14647632 | MsG0880042832.01.T01:CDS |
| AGAAGATGAGATAAATAAGG+AGG | 0.555381 | 8:+14648277 | None:intergenic |
| AGTGGAGAAATAATTTGTGA+AGG | 0.561503 | 8:-14647633 | MsG0880042832.01.T01:CDS |
| TTTGTGAAGGGTATGATGAA+GGG | 0.566441 | 8:-14647620 | MsG0880042832.01.T01:CDS |
| CACAGCATCCATCAAGAACA+AGG | 0.570428 | 8:-14648059 | MsG0880042832.01.T01:intron |
| ATTCTTCAAGAACAAGAAAG+AGG | 0.573168 | 8:+14648235 | None:intergenic |
| TAGACAAATTTCAAGACCAA+CGG | 0.578411 | 8:-14647589 | MsG0880042832.01.T01:CDS |
| TGAAGGTGGTCGTTTGCAAG+AGG | 0.580771 | 8:-14647214 | MsG0880042832.01.T01:CDS |
| TTATTTCTCCACTCTCATCT+CGG | 0.581409 | 8:+14647643 | None:intergenic |
| AAAGTCAACAGTTGGTCCAG+TGG | 0.602548 | 8:-14648092 | MsG0880042832.01.T01:CDS |
| CAAAGCTATGAGTGAGACAA+AGG | 0.622287 | 8:+14647138 | None:intergenic |
| ATTTGCTACCGAGATGAGAG+TGG | 0.626981 | 8:-14647651 | MsG0880042832.01.T01:CDS |
| CTTCAAGAACAAGAAAGAGG+TGG | 0.639571 | 8:+14648238 | None:intergenic |
| ATTGATGATGCAATTGAAGG+TGG | 0.706142 | 8:-14647228 | MsG0880042832.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAGATGAAAAAAAACTT+TGG | - | Chr8:14647361-14647380 | MsG0880042832.01.T01:intron | 15.0% |
| !! | AAGAATAGATAAAAGATAAT+AGG | + | Chr8:14647272-14647291 | None:intergenic | 15.0% |
| !! | ATAGATATATGTATATCAAT+TGG | + | Chr8:14647315-14647334 | None:intergenic | 15.0% |
| !! | TAATTTGAATTGCAATAAAA+TGG | - | Chr8:14648110-14648129 | MsG0880042832.01.T01:CDS | 15.0% |
| !! | TAGATAATATTCAATCAAAA+AGG | - | Chr8:14647746-14647765 | MsG0880042832.01.T01:intron | 15.0% |
| !!! | AAAATGACAATTTTATTCTT+CGG | - | Chr8:14647808-14647827 | MsG0880042832.01.T01:intron | 15.0% |
| !!! | AATTATCATATTTCTGAATT+GGG | + | Chr8:14647128-14647147 | None:intergenic | 15.0% |
| !!! | AGAATAAAATTGTCATTTTA+TGG | + | Chr8:14647807-14647826 | None:intergenic | 15.0% |
| !!! | AGATAAGTTTTTTCTTTTTT+TGG | - | Chr8:14647481-14647500 | MsG0880042832.01.T01:intron | 15.0% |
| !!! | TACCTTATAGTTTTTTTTAA+GGG | + | Chr8:14647449-14647468 | None:intergenic | 15.0% |
| !! | AAATACCAAATTAACATCAT+CGG | + | Chr8:14647875-14647894 | None:intergenic | 20.0% |
| !! | AATTGATGTTTATATTCTGA+AGG | - | Chr8:14647771-14647790 | MsG0880042832.01.T01:intron | 20.0% |
| !! | ATATAAATTTGTCTTACCAA+TGG | - | Chr8:14647967-14647986 | MsG0880042832.01.T01:intron | 20.0% |
| !!! | AAAAAAACTTTGGTTAGTTA+TGG | - | Chr8:14647371-14647390 | MsG0880042832.01.T01:intron | 20.0% |
| !!! | GAATTATCATATTTCTGAAT+TGG | + | Chr8:14647129-14647148 | None:intergenic | 20.0% |
| !!! | GTACCTTATAGTTTTTTTTA+AGG | + | Chr8:14647450-14647469 | None:intergenic | 20.0% |
| !!! | TGGATATATCTACTTTTTAT+TGG | - | Chr8:14647501-14647520 | MsG0880042832.01.T01:intron | 20.0% |
| ! | AATAAGGAGGAAAAATGAAA+TGG | + | Chr8:14647011-14647030 | None:intergenic | 25.0% |
| ! | CTTGAAATTTGTCTATAAGA+TGG | + | Chr8:14647704-14647723 | None:intergenic | 25.0% |
| ! | GTCCCTTAAAAAAAACTATA+AGG | - | Chr8:14647444-14647463 | MsG0880042832.01.T01:intron | 25.0% |
| ! | TGTCTAATTTGATACTAAAC+AGG | - | Chr8:14647595-14647614 | MsG0880042832.01.T01:CDS | 25.0% |
| ! | TTTCTCCTAGTAGTAAAAAT+AGG | - | Chr8:14647537-14647556 | MsG0880042832.01.T01:intron | 25.0% |
| ! | TTTGTCTCTAGTTATATCTT+TGG | - | Chr8:14647418-14647437 | MsG0880042832.01.T01:intron | 25.0% |
| !! | TTCTTGAAGAATTAGCTTAA+TGG | - | Chr8:14647074-14647093 | MsG0880042832.01.T01:three_prime_UTR | 25.0% |
| !! | TTTGGTTAGTTATGGAAATT+TGG | - | Chr8:14647379-14647398 | MsG0880042832.01.T01:intron | 25.0% |
| !!! | ATGTTAATTTGGTATTTTCG+TGG | - | Chr8:14647878-14647897 | MsG0880042832.01.T01:intron | 25.0% |
| !!! | TACTAAACAGGTTTTTTATG+AGG | - | Chr8:14647607-14647626 | MsG0880042832.01.T01:CDS | 25.0% |
| !!! | TGAATCTTCTAAAACAAAGT+TGG | - | Chr8:14648023-14648042 | MsG0880042832.01.T01:intron | 25.0% |
| ACTATAAGGTACGTCAAAAT+TGG | - | Chr8:14647458-14647477 | MsG0880042832.01.T01:intron | 30.0% | |
| AGAAGATGAGATAAATAAGG+AGG | + | Chr8:14647024-14647043 | None:intergenic | 30.0% | |
| AGCAGAACAAAAAATTCCAT+TGG | + | Chr8:14647986-14648005 | None:intergenic | 30.0% | |
| ATTCTTCAAGAACAAGAAAG+AGG | + | Chr8:14647066-14647085 | None:intergenic | 30.0% | |
| GGAAGAAGATGAGATAAATA+AGG | + | Chr8:14647027-14647046 | None:intergenic | 30.0% | |
| TAAAATGAAAAATGTGCTGC+AGG | + | Chr8:14648270-14648289 | None:intergenic | 30.0% | |
| TAGACAAATTTCAAGACCAA+CGG | - | Chr8:14647709-14647728 | MsG0880042832.01.T01:intron | 30.0% | |
| TGCTACCAGTTAAAATCTTT+CGG | - | Chr8:14647839-14647858 | MsG0880042832.01.T01:intron | 30.0% | |
| ! | AGTGGAGAAATAATTTGTGA+AGG | - | Chr8:14647665-14647684 | MsG0880042832.01.T01:CDS | 30.0% |
| ! | AGTTGGTTTCAAATTGCAAA+AGG | - | Chr8:14648040-14648059 | MsG0880042832.01.T01:intron | 30.0% |
| ! | ATGACAATTTTATTCTTCGG+CGG | - | Chr8:14647811-14647830 | MsG0880042832.01.T01:intron | 30.0% |
| ! | GTGGAGAAATAATTTGTGAA+GGG | - | Chr8:14647666-14647685 | MsG0880042832.01.T01:CDS | 30.0% |
| ! | GTTGGTTTCAAATTGCAAAA+GGG | - | Chr8:14648041-14648060 | MsG0880042832.01.T01:intron | 30.0% |
| !! | GAAATTGATGATGCAATTGA+AGG | - | Chr8:14648067-14648086 | MsG0880042832.01.T01:CDS | 30.0% |
| !! | GATTTTAACCTTGTTCTTGA+TGG | + | Chr8:14647250-14647269 | None:intergenic | 30.0% |
| !!! | GGAATTTTTTGTTCTGCTTA+GGG | - | Chr8:14647988-14648007 | MsG0880042832.01.T01:intron | 30.0% |
| !!! | TGGAATTTTTTGTTCTGCTT+AGG | - | Chr8:14647987-14648006 | MsG0880042832.01.T01:intron | 30.0% |
| CCAAAGAAAAAGTCAACAGT+TGG | - | Chr8:14647198-14647217 | MsG0880042832.01.T01:exon | 35.0% | |
| CCTAGTAGTAAAAATAGGCT+AGG | - | Chr8:14647542-14647561 | MsG0880042832.01.T01:intron | 35.0% | |
| TACTGCCGATGATGTTAATT+TGG | - | Chr8:14647867-14647886 | MsG0880042832.01.T01:intron | 35.0% | |
| TCAAATAGCACATGTGTAGT+AGG | - | Chr8:14648238-14648257 | MsG0880042832.01.T01:five_prime_UTR | 35.0% | |
| TTCCTAGCATTCTTTGATGT+CGG | - | Chr8:14647904-14647923 | MsG0880042832.01.T01:intron | 35.0% | |
| ! | ATTTGTGAAGGGTATGATGA+AGG | - | Chr8:14647677-14647696 | MsG0880042832.01.T01:CDS | 35.0% |
| ! | TTATTTCTCCACTCTCATCT+CGG | + | Chr8:14647658-14647677 | None:intergenic | 35.0% |
| ! | TTTGTGAAGGGTATGATGAA+GGG | - | Chr8:14647678-14647697 | MsG0880042832.01.T01:CDS | 35.0% |
| ! | TTTTATGAGGATGAGTCTCA+AGG | - | Chr8:14647620-14647639 | MsG0880042832.01.T01:CDS | 35.0% |
| !! | AACTGCCGAAAGATTTTAAC+TGG | + | Chr8:14647847-14647866 | None:intergenic | 35.0% |
| !! | ATTGATGATGCAATTGAAGG+TGG | - | Chr8:14648070-14648089 | MsG0880042832.01.T01:CDS | 35.0% |
| !!! | CCAACTGTTGACTTTTTCTT+TGG | + | Chr8:14647201-14647220 | None:intergenic | 35.0% |
| !!! | CCTAGCCTATTTTTACTACT+AGG | + | Chr8:14647545-14647564 | None:intergenic | 35.0% |
| ATAGGCTAGGACTGAGATTA+AGG | - | Chr8:14647555-14647574 | MsG0880042832.01.T01:intron | 40.0% | |
| CAAAGCTATGAGTGAGACAA+AGG | + | Chr8:14648163-14648182 | None:intergenic | 40.0% | |
| CTTCAAGAACAAGAAAGAGG+TGG | + | Chr8:14647063-14647082 | None:intergenic | 40.0% | |
| TTCCGACATCAAAGAATGCT+AGG | + | Chr8:14647909-14647928 | None:intergenic | 40.0% | |
| ! | GCTGTGATTTTCAATACCAC+TGG | + | Chr8:14647225-14647244 | None:intergenic | 40.0% |
| ! | TACCTTTTGTGATGAACCGT+TGG | + | Chr8:14647728-14647747 | None:intergenic | 40.0% |
| !! | CACTCATAGCTTTGAGTTTG+AGG | - | Chr8:14648169-14648188 | MsG0880042832.01.T01:CDS | 40.0% |
| AAAGTCAACAGTTGGTCCAG+TGG | - | Chr8:14647206-14647225 | MsG0880042832.01.T01:CDS | 45.0% | |
| AGAGGTGGAAGAAGAAGATG+TGG | + | Chr8:14647048-14647067 | None:intergenic | 45.0% | |
| CACAGCATCCATCAAGAACA+AGG | - | Chr8:14647239-14647258 | MsG0880042832.01.T01:CDS | 45.0% | |
| GACCAACGGTTCATCACAAA+AGG | - | Chr8:14647723-14647742 | MsG0880042832.01.T01:intron | 45.0% | |
| ! | ATTTGCTACCGAGATGAGAG+TGG | - | Chr8:14647647-14647666 | MsG0880042832.01.T01:CDS | 45.0% |
| !! | CTTTGGAGCATAGCATCGTT+TGG | + | Chr8:14647184-14647203 | None:intergenic | 45.0% |
| TGAAGGTGGTCGTTTGCAAG+AGG | - | Chr8:14648084-14648103 | MsG0880042832.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 14646992 | 14648328 | 14646992 | ID=MsG0880042832.01;Name=MsG0880042832.01 |
| Chr8 | mRNA | 14646992 | 14648328 | 14646992 | ID=MsG0880042832.01.T01;Parent=MsG0880042832.01;Name=MsG0880042832.01.T01;_AED=0.26;_eAED=0.26;_QI=101|1|1|1|1|1|3|211|134 |
| Chr8 | exon | 14646992 | 14647311 | 14646992 | ID=MsG0880042832.01.T01:exon:13541;Parent=MsG0880042832.01.T01 |
| Chr8 | exon | 14647576 | 14647703 | 14647576 | ID=MsG0880042832.01.T01:exon:13540;Parent=MsG0880042832.01.T01 |
| Chr8 | exon | 14648060 | 14648328 | 14648060 | ID=MsG0880042832.01.T01:exon:13539;Parent=MsG0880042832.01.T01 |
| Chr8 | five_prime_UTR | 14648228 | 14648328 | 14648228 | ID=MsG0880042832.01.T01:five_prime_utr;Parent=MsG0880042832.01.T01 |
| Chr8 | CDS | 14648060 | 14648227 | 14648060 | ID=MsG0880042832.01.T01:cds;Parent=MsG0880042832.01.T01 |
| Chr8 | CDS | 14647576 | 14647703 | 14647576 | ID=MsG0880042832.01.T01:cds;Parent=MsG0880042832.01.T01 |
| Chr8 | CDS | 14647203 | 14647311 | 14647203 | ID=MsG0880042832.01.T01:cds;Parent=MsG0880042832.01.T01 |
| Chr8 | three_prime_UTR | 14646992 | 14647202 | 14646992 | ID=MsG0880042832.01.T01:three_prime_utr;Parent=MsG0880042832.01.T01 |
| Gene Sequence |
| Protein sequence |
