Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031180.01.T04 | QGA85355.1 | 96.875 | 32 | 1 | 0 | 1 | 32 | 51 | 82 | 2.17E-13 | 67.8 |
| MsG0680031180.01.T02 | XP_039683458.1 | 92.857 | 70 | 5 | 0 | 1 | 70 | 111 | 180 | 8.32E-41 | 142 |
| MsG0680031180.01.T03 | XP_039683458.1 | 92.308 | 65 | 5 | 0 | 1 | 65 | 116 | 180 | 6.87E-37 | 132 |
| MsG0680031180.01.T01 | XP_039683458.1 | 93.243 | 74 | 5 | 0 | 6 | 79 | 107 | 180 | 1.02E-43 | 149 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031180.01.T01 | B5LML3 | 91.892 | 74 | 6 | 0 | 6 | 79 | 75 | 148 | 1.56E-45 | 145 |
| MsG0680031180.01.T02 | B5LML3 | 91.429 | 70 | 6 | 0 | 1 | 70 | 79 | 148 | 1.13E-42 | 138 |
| MsG0680031180.01.T03 | B5LML3 | 90.769 | 65 | 6 | 0 | 1 | 65 | 84 | 148 | 1.01E-38 | 128 |
| MsG0680031180.01.T04 | Q09G43 | 93.75 | 32 | 2 | 0 | 1 | 32 | 117 | 148 | 1.74E-15 | 67.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031180.01.T01 | B8R4A5 | 93.243 | 74 | 5 | 0 | 6 | 79 | 75 | 148 | 1.08e-43 | 147 |
| MsG0680031180.01.T03 | W8CYE2 | 92.308 | 65 | 5 | 0 | 1 | 65 | 84 | 148 | 7.50e-37 | 130 |
| MsG0680031180.01.T04 | A0A5Q0UVN1 | 96.875 | 32 | 1 | 0 | 1 | 32 | 51 | 82 | 1.03e-13 | 67.8 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0380013922.01 | MsG0680031180.01 | 0.809522 | 1.815363e-50 | 1.068223e-47 |
| MsG0580024908.01 | MsG0680031180.01 | 0.879664 | 1.036021e-69 | 5.665162e-66 |
| MsG0380016059.01 | MsG0680031180.01 | 0.829295 | 5.577161e-55 | 5.685280e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031180.01.T04 | MTR_7g021800 | 93.750 | 32 | 2 | 0 | 1 | 32 | 117 | 148 | 2.52e-16 | 67.0 |
| MsG0680031180.01.T04 | MTR_4g035040 | 93.750 | 32 | 2 | 0 | 1 | 32 | 117 | 148 | 2.52e-16 | 67.0 |
| MsG0680031180.01.T04 | MTR_0002s0630 | 90.625 | 32 | 3 | 0 | 1 | 32 | 277 | 308 | 1.30e-15 | 67.4 |
| MsG0680031180.01.T02 | MTR_7g021800 | 92.857 | 70 | 5 | 0 | 1 | 70 | 79 | 148 | 2.40e-44 | 140 |
| MsG0680031180.01.T02 | MTR_4g035040 | 92.857 | 70 | 5 | 0 | 1 | 70 | 79 | 148 | 2.40e-44 | 140 |
| MsG0680031180.01.T02 | MTR_0002s0630 | 82.857 | 70 | 12 | 0 | 1 | 70 | 239 | 308 | 2.24e-39 | 132 |
| MsG0680031180.01.T03 | MTR_7g021800 | 92.308 | 65 | 5 | 0 | 1 | 65 | 84 | 148 | 1.90e-40 | 130 |
| MsG0680031180.01.T03 | MTR_4g035040 | 92.308 | 65 | 5 | 0 | 1 | 65 | 84 | 148 | 1.90e-40 | 130 |
| MsG0680031180.01.T03 | MTR_0002s0630 | 83.077 | 65 | 11 | 0 | 1 | 65 | 244 | 308 | 3.32e-36 | 123 |
| MsG0680031180.01.T01 | MTR_7g021800 | 93.243 | 74 | 5 | 0 | 6 | 79 | 75 | 148 | 3.08e-47 | 147 |
| MsG0680031180.01.T01 | MTR_4g035040 | 93.243 | 74 | 5 | 0 | 6 | 79 | 75 | 148 | 3.08e-47 | 147 |
| MsG0680031180.01.T01 | MTR_0002s0630 | 79.730 | 74 | 15 | 0 | 6 | 79 | 235 | 308 | 6.03e-41 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680031180.01.T04 | ATCG00420 | 87.500 | 32 | 4 | 0 | 1 | 32 | 117 | 148 | 1.76e-15 | 64.7 |
| MsG0680031180.01.T02 | ATCG00420 | 81.429 | 70 | 13 | 0 | 1 | 70 | 79 | 148 | 3.24e-39 | 127 |
| MsG0680031180.01.T03 | ATCG00420 | 83.077 | 65 | 11 | 0 | 1 | 65 | 84 | 148 | 8.52e-37 | 120 |
| MsG0680031180.01.T01 | ATCG00420 | 79.730 | 74 | 15 | 0 | 6 | 79 | 75 | 148 | 4.06e-41 | 132 |
Find 9 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCATCCTATCCAACTTTC+GGG | 0.254679 | 6:-15654418 | None:intergenic |
| AGGCCATCCTATCCAACTTT+CGG | 0.340000 | 6:-15654419 | None:intergenic |
| CAGAAAATAGAAGGAATTCT+AGG | 0.348933 | 6:-15654301 | None:intergenic |
| TTCCAAACCCAGAAAATAGA+AGG | 0.366444 | 6:-15654310 | None:intergenic |
| AGGGAATCTTATGATATGTT+AGG | 0.415137 | 6:+15654352 | MsG0680031180.01.T01:CDS |
| TGCCCGAAAGTTGGATAGGA+TGG | 0.516198 | 6:+15654416 | MsG0680031180.01.T01:CDS |
| TTAATGCCCGAAAGTTGGAT+AGG | 0.556642 | 6:+15654412 | MsG0680031180.01.T01:CDS |
| GAGATATAATCTTTATGCAA+AGG | 0.598735 | 6:-15654439 | None:intergenic |
| ATTAAAATACGTTTCAGACG+CGG | 0.619896 | 6:-15654394 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATTTTTATGCATACTTCTT+CGG | - | Chr6:15654263-15654282 | None:intergenic | 20.0% |
| ! | GAGATATAATCTTTATGCAA+AGG | - | Chr6:15654442-15654461 | None:intergenic | 25.0% |
| !! | ATTTTTATGCATACTTCTTC+GGG | - | Chr6:15654262-15654281 | None:intergenic | 25.0% |
| !! | GCATAAAAATATTTTTCCCA+TGG | + | Chr6:15654272-15654291 | MsG0680031180.01.T01:CDS | 25.0% |
| !! | TAGAATTCCTTCTATTTTCT+GGG | + | Chr6:15654303-15654322 | MsG0680031180.01.T01:CDS | 25.0% |
| AGGGAATCTTATGATATGTT+AGG | + | Chr6:15654352-15654371 | MsG0680031180.01.T01:CDS | 30.0% | |
| ATTAAAATACGTTTCAGACG+CGG | - | Chr6:15654397-15654416 | None:intergenic | 30.0% | |
| CAGAAAATAGAAGGAATTCT+AGG | - | Chr6:15654304-15654323 | None:intergenic | 30.0% | |
| ! | CTAGAATTCCTTCTATTTTC+TGG | + | Chr6:15654302-15654321 | MsG0680031180.01.T01:CDS | 30.0% |
| !!! | AAAGTGCTGATTTTCAAGAA+AGG | + | Chr6:15654332-15654351 | MsG0680031180.01.T01:CDS | 30.0% |
| !!! | AAGTGCTGATTTTCAAGAAA+GGG | + | Chr6:15654333-15654352 | MsG0680031180.01.T01:CDS | 30.0% |
| TTCCAAACCCAGAAAATAGA+AGG | - | Chr6:15654313-15654332 | None:intergenic | 35.0% | |
| ! | TTCCTTCTATTTTCTGGGTT+TGG | + | Chr6:15654308-15654327 | MsG0680031180.01.T01:CDS | 35.0% |
| !!! | GAATTCTAGGATTTTGCCAT+GGG | - | Chr6:15654291-15654310 | None:intergenic | 35.0% |
| !!! | GTATTTTAATGCCCGAAAGT+TGG | + | Chr6:15654407-15654426 | MsG0680031180.01.T01:CDS | 35.0% |
| TTAATGCCCGAAAGTTGGAT+AGG | + | Chr6:15654412-15654431 | MsG0680031180.01.T01:CDS | 40.0% | |
| !!! | GGAATTCTAGGATTTTGCCA+TGG | - | Chr6:15654292-15654311 | None:intergenic | 40.0% |
| AGGCCATCCTATCCAACTTT+CGG | - | Chr6:15654422-15654441 | None:intergenic | 45.0% | |
| GGCCATCCTATCCAACTTTC+GGG | - | Chr6:15654421-15654440 | None:intergenic | 50.0% | |
| TGCCCGAAAGTTGGATAGGA+TGG | + | Chr6:15654416-15654435 | MsG0680031180.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 15654226 | 15654474 | 15654226 | ID=MsG0680031180.01;Name=MsG0680031180.01 |
| Chr6 | mRNA | 15654226 | 15654474 | 15654226 | ID=MsG0680031180.01.T01;Parent=MsG0680031180.01;Name=MsG0680031180.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|82 |
| Chr6 | exon | 15654226 | 15654474 | 15654226 | ID=MsG0680031180.01.T01:exon:10546;Parent=MsG0680031180.01.T01 |
| Chr6 | CDS | 15654226 | 15654474 | 15654226 | ID=MsG0680031180.01.T01:cds;Parent=MsG0680031180.01.T01 |
| Chr6 | mRNA | 15654253 | 15654474 | 15654253 | ID=MsG0680031180.01.T02;Parent=MsG0680031180.01;Name=MsG0680031180.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|73 |
| Chr6 | exon | 15654253 | 15654474 | 15654253 | ID=MsG0680031180.01.T02:exon:10547;Parent=MsG0680031180.01.T02 |
| Chr6 | CDS | 15654253 | 15654474 | 15654253 | ID=MsG0680031180.01.T02:cds;Parent=MsG0680031180.01.T02 |
| Chr6 | mRNA | 15654268 | 15654474 | 15654268 | ID=MsG0680031180.01.T03;Parent=MsG0680031180.01;Name=MsG0680031180.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|68 |
| Chr6 | exon | 15654268 | 15654474 | 15654268 | ID=MsG0680031180.01.T03:exon:10548;Parent=MsG0680031180.01.T03 |
| Chr6 | CDS | 15654268 | 15654474 | 15654268 | ID=MsG0680031180.01.T03:cds;Parent=MsG0680031180.01.T03 |
| Chr6 | mRNA | 15654367 | 15654474 | 15654367 | ID=MsG0680031180.01.T04;Parent=MsG0680031180.01;Name=MsG0680031180.01.T04;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|35 |
| Chr6 | exon | 15654367 | 15654474 | 15654367 | ID=MsG0680031180.01.T04:exon:10549;Parent=MsG0680031180.01.T04 |
| Chr6 | CDS | 15654367 | 15654474 | 15654367 | ID=MsG0680031180.01.T04:cds;Parent=MsG0680031180.01.T04 |
| Chr6 | mRNA | 15654415 | 15654474 | 15654415 | ID=MsG0680031180.01.T05;Parent=MsG0680031180.01;Name=MsG0680031180.01.T05;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|19 |
| Chr6 | exon | 15654415 | 15654474 | 15654415 | ID=MsG0680031180.01.T05:exon:10550;Parent=MsG0680031180.01.T05 |
| Chr6 | CDS | 15654415 | 15654474 | 15654415 | ID=MsG0680031180.01.T05:cds;Parent=MsG0680031180.01.T05 |
| Gene Sequence |
| Protein sequence |
