Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032220.01.T01 | XP_013451922.1 | 94.949 | 198 | 10 | 0 | 1 | 198 | 26 | 223 | 1.04E-131 | 388 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032220.01.T01 | O22822 | 41.117 | 197 | 106 | 3 | 3 | 197 | 24 | 212 | 5.97E-44 | 155 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032220.01.T01 | A0A072UJF5 | 94.949 | 198 | 10 | 0 | 1 | 198 | 26 | 223 | 4.95e-132 | 388 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002809.01 | MsG0680032220.01 | 0.804701 | 1.904189e-49 | 9.884758e-47 |
| MsG0680032220.01 | MsG0780040158.01 | 0.811001 | 8.706398e-51 | 5.328263e-48 |
| MsG0680032220.01 | MsG0880046854.01 | 0.809110 | 2.225250e-50 | 1.295153e-47 |
| MsG0480018844.01 | MsG0680032220.01 | 0.810413 | 1.167331e-50 | 7.031551e-48 |
| MsG0480021308.01 | MsG0680032220.01 | 0.826824 | 2.194603e-54 | 2.080944e-51 |
| MsG0380016896.01 | MsG0680032220.01 | 0.801710 | 7.917605e-49 | 3.807193e-46 |
| MsG0280010356.01 | MsG0680032220.01 | 0.801681 | 8.028430e-49 | 3.857542e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032220.01.T01 | MTR_6g038300 | 94.949 | 198 | 10 | 0 | 1 | 198 | 26 | 223 | 1.26e-135 | 388 |
| MsG0680032220.01.T01 | MTR_6g038240 | 68.342 | 199 | 61 | 2 | 1 | 198 | 26 | 223 | 2.72e-93 | 280 |
| MsG0680032220.01.T01 | MTR_6g038220 | 62.312 | 199 | 74 | 1 | 1 | 199 | 26 | 223 | 4.05e-89 | 269 |
| MsG0680032220.01.T01 | MTR_6g038200 | 61.809 | 199 | 76 | 0 | 1 | 199 | 26 | 224 | 1.38e-86 | 263 |
| MsG0680032220.01.T01 | MTR_6g038230 | 59.596 | 198 | 80 | 0 | 1 | 198 | 26 | 223 | 2.20e-81 | 249 |
| MsG0680032220.01.T01 | MTR_7g080960 | 47.449 | 196 | 101 | 2 | 1 | 195 | 27 | 221 | 9.21e-59 | 192 |
| MsG0680032220.01.T01 | MTR_7g080900 | 47.716 | 197 | 101 | 2 | 1 | 196 | 27 | 222 | 1.49e-58 | 191 |
| MsG0680032220.01.T01 | MTR_7g080935 | 46.939 | 196 | 102 | 1 | 1 | 196 | 26 | 219 | 1.72e-55 | 183 |
| MsG0680032220.01.T01 | MTR_7g080940 | 46.231 | 199 | 105 | 1 | 1 | 199 | 26 | 222 | 1.72e-55 | 182 |
| MsG0680032220.01.T01 | MTR_8g088560 | 44.949 | 198 | 107 | 1 | 1 | 196 | 25 | 222 | 7.56e-54 | 179 |
| MsG0680032220.01.T01 | MTR_7g080950 | 44.898 | 196 | 106 | 1 | 1 | 196 | 21 | 214 | 8.15e-53 | 176 |
| MsG0680032220.01.T01 | MTR_5g035580 | 42.268 | 194 | 108 | 1 | 1 | 194 | 28 | 217 | 7.25e-45 | 155 |
| MsG0680032220.01.T01 | MTR_5g035560 | 40.206 | 194 | 112 | 1 | 1 | 194 | 26 | 215 | 2.55e-42 | 149 |
| MsG0680032220.01.T01 | MTR_8g083290 | 32.367 | 207 | 121 | 8 | 2 | 194 | 20 | 221 | 5.31e-22 | 93.2 |
| MsG0680032220.01.T01 | MTR_3g084520 | 31.658 | 199 | 127 | 4 | 2 | 191 | 22 | 220 | 7.86e-21 | 90.1 |
| MsG0680032220.01.T01 | MTR_1g107380 | 28.505 | 214 | 131 | 5 | 1 | 196 | 28 | 237 | 6.09e-20 | 87.4 |
| MsG0680032220.01.T01 | MTR_4g095008 | 30.693 | 202 | 127 | 6 | 1 | 194 | 26 | 222 | 4.13e-19 | 85.1 |
| MsG0680032220.01.T01 | MTR_1g032490 | 26.794 | 209 | 142 | 4 | 1 | 198 | 25 | 233 | 1.90e-17 | 80.5 |
| MsG0680032220.01.T01 | MTR_3g049970 | 27.273 | 220 | 145 | 5 | 1 | 207 | 24 | 241 | 4.65e-17 | 79.3 |
| MsG0680032220.01.T01 | MTR_3g084530 | 28.641 | 206 | 130 | 5 | 2 | 194 | 22 | 223 | 1.52e-16 | 77.8 |
| MsG0680032220.01.T01 | MTR_1g107315 | 26.794 | 209 | 142 | 4 | 1 | 198 | 23 | 231 | 2.07e-16 | 77.4 |
| MsG0680032220.01.T01 | MTR_1g107360 | 24.402 | 209 | 147 | 3 | 1 | 198 | 24 | 232 | 4.80e-16 | 76.3 |
| MsG0680032220.01.T01 | MTR_8g064880 | 26.364 | 220 | 149 | 5 | 1 | 207 | 24 | 243 | 9.24e-15 | 72.4 |
| MsG0680032220.01.T01 | MTR_4g094920 | 28.000 | 200 | 133 | 5 | 2 | 194 | 42 | 237 | 1.03e-14 | 72.4 |
| MsG0680032220.01.T01 | MTR_1g107375 | 25.604 | 207 | 140 | 4 | 1 | 198 | 23 | 224 | 1.50e-14 | 72.0 |
| MsG0680032220.01.T01 | MTR_8g083280 | 28.910 | 211 | 132 | 8 | 2 | 194 | 23 | 233 | 1.33e-13 | 69.3 |
| MsG0680032220.01.T01 | MTR_1g107350 | 24.402 | 209 | 147 | 4 | 1 | 198 | 23 | 231 | 3.81e-13 | 67.8 |
| MsG0680032220.01.T01 | MTR_1g107365 | 23.585 | 212 | 140 | 3 | 1 | 198 | 60 | 263 | 5.90e-13 | 67.4 |
| MsG0680032220.01.T01 | MTR_8g064870 | 25.121 | 207 | 142 | 5 | 1 | 196 | 26 | 230 | 2.20e-11 | 62.8 |
| MsG0680032220.01.T01 | MTR_8g083300 | 27.119 | 118 | 85 | 1 | 2 | 118 | 23 | 140 | 5.18e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032220.01.T01 | AT2G43820 | 41.117 | 197 | 106 | 3 | 3 | 197 | 24 | 212 | 6.09e-45 | 155 |
| MsG0680032220.01.T01 | AT2G43840 | 41.206 | 199 | 105 | 4 | 3 | 198 | 24 | 213 | 1.09e-43 | 152 |
| MsG0680032220.01.T01 | AT2G43840 | 40.704 | 199 | 106 | 4 | 3 | 198 | 24 | 213 | 9.19e-43 | 149 |
| MsG0680032220.01.T01 | AT1G05680 | 35.897 | 195 | 119 | 3 | 1 | 191 | 21 | 213 | 6.77e-28 | 109 |
| MsG0680032220.01.T01 | AT1G05675 | 35.385 | 195 | 120 | 3 | 1 | 191 | 21 | 213 | 6.51e-27 | 107 |
| MsG0680032220.01.T01 | AT1G24100 | 33.333 | 195 | 122 | 4 | 1 | 191 | 26 | 216 | 2.82e-26 | 105 |
| MsG0680032220.01.T01 | AT1G05560 | 30.808 | 198 | 126 | 4 | 2 | 194 | 21 | 212 | 3.25e-23 | 96.7 |
| MsG0680032220.01.T01 | AT1G05560 | 30.808 | 198 | 126 | 4 | 2 | 194 | 71 | 262 | 3.99e-23 | 96.7 |
| MsG0680032220.01.T01 | AT2G31790 | 30.303 | 198 | 131 | 3 | 1 | 194 | 23 | 217 | 6.28e-23 | 95.9 |
| MsG0680032220.01.T01 | AT4G15550 | 31.925 | 213 | 123 | 7 | 2 | 194 | 29 | 239 | 1.23e-21 | 92.4 |
| MsG0680032220.01.T01 | AT2G31750 | 33.981 | 206 | 112 | 7 | 1 | 194 | 23 | 216 | 2.13e-21 | 91.7 |
| MsG0680032220.01.T01 | AT2G31750 | 33.981 | 206 | 112 | 7 | 1 | 194 | 23 | 216 | 3.26e-21 | 91.3 |
| MsG0680032220.01.T01 | AT4G15490 | 26.761 | 213 | 135 | 7 | 1 | 196 | 23 | 231 | 3.38e-20 | 88.2 |
| MsG0680032220.01.T01 | AT4G15480 | 26.066 | 211 | 142 | 5 | 1 | 198 | 34 | 243 | 2.91e-19 | 85.5 |
| MsG0680032220.01.T01 | AT3G21560 | 23.223 | 211 | 146 | 5 | 1 | 196 | 27 | 236 | 3.54e-16 | 76.6 |
| MsG0680032220.01.T01 | AT1G05530 | 27.638 | 199 | 129 | 5 | 2 | 193 | 21 | 211 | 1.24e-15 | 75.1 |
| MsG0680032220.01.T01 | AT2G23210 | 26.531 | 196 | 129 | 6 | 1 | 191 | 25 | 210 | 3.24e-15 | 72.8 |
| MsG0680032220.01.T01 | AT2G23250 | 27.000 | 200 | 130 | 6 | 1 | 194 | 13 | 202 | 8.66e-15 | 72.8 |
| MsG0680032220.01.T01 | AT2G28080 | 24.638 | 207 | 138 | 5 | 2 | 191 | 34 | 239 | 1.11e-13 | 69.3 |
| MsG0680032220.01.T01 | AT2G23260 | 31.690 | 142 | 86 | 4 | 57 | 194 | 81 | 215 | 8.34e-13 | 67.0 |
| MsG0680032220.01.T01 | AT4G14090 | 23.590 | 195 | 143 | 3 | 2 | 191 | 29 | 222 | 3.21e-12 | 65.1 |
| MsG0680032220.01.T01 | AT1G22340 | 24.651 | 215 | 141 | 6 | 1 | 194 | 28 | 242 | 3.33e-12 | 65.1 |
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGATCAATCTTTGAAAAT+TGG | 0.136693 | 6:+36652468 | None:intergenic |
| AATCTTACAAAGACACTTTC+TGG | 0.150707 | 6:-36652811 | MsG0680032220.01.T01:CDS |
| CCTTCTTTCTTGTATCAATA+TGG | 0.199884 | 6:-36652524 | MsG0680032220.01.T01:CDS |
| TTTGATGTCTCCAAGAGTTT+TGG | 0.276354 | 6:-36652689 | MsG0680032220.01.T01:CDS |
| TCAAAACGTTTAGCCCAAAA+AGG | 0.302682 | 6:-36652968 | MsG0680032220.01.T01:CDS |
| ACAATTTCGCTCTTCGAAAT+CGG | 0.344070 | 6:+36652584 | None:intergenic |
| AAATCAGCAGGTTGGAGCTT+TGG | 0.359401 | 6:+36652548 | None:intergenic |
| CAAAGATTGATCAGGCTGAT+TGG | 0.362084 | 6:-36652460 | MsG0680032220.01.T01:CDS |
| AAAGATTGATCAGGCTGATT+GGG | 0.399248 | 6:-36652459 | MsG0680032220.01.T01:CDS |
| AAAGACACTTTCTGGAAAGT+TGG | 0.411115 | 6:-36652803 | MsG0680032220.01.T01:CDS |
| TCATAGACAACACAATCAAC+AGG | 0.420837 | 6:+36652731 | None:intergenic |
| TGAGTTAAGAAAACAGCAAC+AGG | 0.452642 | 6:+36652662 | None:intergenic |
| TAGTGAACACCATTTCCATT+TGG | 0.456695 | 6:-36652931 | MsG0680032220.01.T01:CDS |
| GGTTGGAGCTTTGGCAATCC+AGG | 0.457185 | 6:+36652557 | None:intergenic |
| TATCAATATGGAACCTATCC+TGG | 0.464951 | 6:-36652512 | MsG0680032220.01.T01:CDS |
| AAATGCTAGTGTCATATTTG+TGG | 0.466537 | 6:+36652362 | None:intergenic |
| AACAGGAATTCCAAAACTCT+TGG | 0.485454 | 6:+36652679 | None:intergenic |
| TCTATGATGCTTTCTTACAT+TGG | 0.487232 | 6:-36652715 | MsG0680032220.01.T01:CDS |
| AAGAGCGAAATTGTGTTACC+TGG | 0.503915 | 6:-36652575 | MsG0680032220.01.T01:CDS |
| ATAAGTAAATCACTTACCTC+TGG | 0.511051 | 6:+36652407 | None:intergenic |
| AATATCAAAGTAACCAGGAT+AGG | 0.527436 | 6:+36652499 | None:intergenic |
| CCATATTGATACAAGAAAGA+AGG | 0.534781 | 6:+36652524 | None:intergenic |
| AAGAAAGAAGGCAAATCAGC+AGG | 0.535346 | 6:+36652536 | None:intergenic |
| GAAACTGAAAGCATTTCAGA+TGG | 0.546294 | 6:-36652872 | MsG0680032220.01.T01:CDS |
| AAGAAGGCAAATCAGCAGGT+TGG | 0.549618 | 6:+36652540 | None:intergenic |
| AATGTCATCAGCAGAAAACA+TGG | 0.565111 | 6:-36652834 | MsG0680032220.01.T01:CDS |
| ATTTCAGATGGTTATGACAA+TGG | 0.599843 | 6:-36652860 | MsG0680032220.01.T01:CDS |
| TCAGATGGTTATGACAATGG+AGG | 0.652364 | 6:-36652857 | MsG0680032220.01.T01:CDS |
| GTAAGAATATCAAAGTAACC+AGG | 0.674674 | 6:+36652494 | None:intergenic |
| CATAGACAACACAATCAACA+GGG | 0.697199 | 6:+36652732 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTCATGCTTTTATGAAA+TGG | - | Chr6:36652737-36652756 | MsG0680032220.01.T01:CDS | 20.0% |
| !!! | TGTTATTTTTACTCCTTTTT+GGG | + | Chr6:36652401-36652420 | None:intergenic | 20.0% |
| !!! | GTGTTATTTTTACTCCTTTT+TGG | + | Chr6:36652402-36652421 | None:intergenic | 25.0% |
| AATATCAAAGTAACCAGGAT+AGG | + | Chr6:36652857-36652876 | None:intergenic | 30.0% | |
| AATCTTACAAAGACACTTTC+TGG | - | Chr6:36652542-36652561 | MsG0680032220.01.T01:CDS | 30.0% | |
| ATAAGTAAATCACTTACCTC+TGG | + | Chr6:36652949-36652968 | None:intergenic | 30.0% | |
| ATTTCAGATGGTTATGACAA+TGG | - | Chr6:36652493-36652512 | MsG0680032220.01.T01:CDS | 30.0% | |
| CCATATTGATACAAGAAAGA+AGG | + | Chr6:36652832-36652851 | None:intergenic | 30.0% | |
| CCTTCTTTCTTGTATCAATA+TGG | - | Chr6:36652829-36652848 | MsG0680032220.01.T01:CDS | 30.0% | |
| GTAAGAATATCAAAGTAACC+AGG | + | Chr6:36652862-36652881 | None:intergenic | 30.0% | |
| TCTATGATGCTTTCTTACAT+TGG | - | Chr6:36652638-36652657 | MsG0680032220.01.T01:CDS | 30.0% | |
| ! | CCAATTTTCAAAGATTGATC+AGG | - | Chr6:36652885-36652904 | MsG0680032220.01.T01:CDS | 30.0% |
| ! | CCTGATCAATCTTTGAAAAT+TGG | + | Chr6:36652888-36652907 | None:intergenic | 30.0% |
| ! | GATTTTGTTCCAAATGGAAA+TGG | + | Chr6:36652434-36652453 | None:intergenic | 30.0% |
| !!! | AGAAGTTGAGAAAGTGTTTT+TGG | + | Chr6:36652577-36652596 | None:intergenic | 30.0% |
| !!! | GTTGTTGATTTTGTTCCAAA+TGG | + | Chr6:36652440-36652459 | None:intergenic | 30.0% |
| AAAGATTGATCAGGCTGATT+GGG | - | Chr6:36652894-36652913 | MsG0680032220.01.T01:CDS | 35.0% | |
| AACAGGAATTCCAAAACTCT+TGG | + | Chr6:36652677-36652696 | None:intergenic | 35.0% | |
| AATGTCATCAGCAGAAAACA+TGG | - | Chr6:36652519-36652538 | MsG0680032220.01.T01:CDS | 35.0% | |
| ACAATTTCGCTCTTCGAAAT+CGG | + | Chr6:36652772-36652791 | None:intergenic | 35.0% | |
| CATAGACAACACAATCAACA+GGG | + | Chr6:36652624-36652643 | None:intergenic | 35.0% | |
| TAGTGAACACCATTTCCATT+TGG | - | Chr6:36652422-36652441 | MsG0680032220.01.T01:CDS | 35.0% | |
| TATCAATATGGAACCTATCC+TGG | - | Chr6:36652841-36652860 | MsG0680032220.01.T01:CDS | 35.0% | |
| TCAAAACGTTTAGCCCAAAA+AGG | - | Chr6:36652385-36652404 | MsG0680032220.01.T01:CDS | 35.0% | |
| TCATAGACAACACAATCAAC+AGG | + | Chr6:36652625-36652644 | None:intergenic | 35.0% | |
| TGAGTTAAGAAAACAGCAAC+AGG | + | Chr6:36652694-36652713 | None:intergenic | 35.0% | |
| ! | AAAGACACTTTCTGGAAAGT+TGG | - | Chr6:36652550-36652569 | MsG0680032220.01.T01:CDS | 35.0% |
| ! | TTTGATGTCTCCAAGAGTTT+TGG | - | Chr6:36652664-36652683 | MsG0680032220.01.T01:CDS | 35.0% |
| !! | GAAACTGAAAGCATTTCAGA+TGG | - | Chr6:36652481-36652500 | MsG0680032220.01.T01:CDS | 35.0% |
| AAGAAAGAAGGCAAATCAGC+AGG | + | Chr6:36652820-36652839 | None:intergenic | 40.0% | |
| AAGAGCGAAATTGTGTTACC+TGG | - | Chr6:36652778-36652797 | MsG0680032220.01.T01:CDS | 40.0% | |
| CAAAGATTGATCAGGCTGAT+TGG | - | Chr6:36652893-36652912 | MsG0680032220.01.T01:CDS | 40.0% | |
| TCAGATGGTTATGACAATGG+AGG | - | Chr6:36652496-36652515 | MsG0680032220.01.T01:CDS | 40.0% | |
| AAGAAGGCAAATCAGCAGGT+TGG | + | Chr6:36652816-36652835 | None:intergenic | 45.0% | |
| ! | AAATCAGCAGGTTGGAGCTT+TGG | + | Chr6:36652808-36652827 | None:intergenic | 45.0% |
| ! | GTTTTACGAACTTGAGCCAG+AGG | - | Chr6:36652930-36652949 | MsG0680032220.01.T01:CDS | 45.0% |
| ! | GGTTGGAGCTTTGGCAATCC+AGG | + | Chr6:36652799-36652818 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 36652373 | 36653002 | 36652373 | ID=MsG0680032220.01;Name=MsG0680032220.01 |
| Chr6 | mRNA | 36652373 | 36653002 | 36652373 | ID=MsG0680032220.01.T01;Parent=MsG0680032220.01;Name=MsG0680032220.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|209 |
| Chr6 | exon | 36652373 | 36653002 | 36652373 | ID=MsG0680032220.01.T01:exon:19883;Parent=MsG0680032220.01.T01 |
| Chr6 | CDS | 36652373 | 36653002 | 36652373 | ID=MsG0680032220.01.T01:cds;Parent=MsG0680032220.01.T01 |
| Gene Sequence |
| Protein sequence |
