Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033159.01.T01 | XP_003599555.1 | 97.087 | 515 | 15 | 0 | 1 | 515 | 1 | 515 | 0 | 1028 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0680033089.01 | MsG0680033159.01 | 0.968182 | 2.577777e-128 | 2.098735e-122 |
| MsG0680033146.01 | MsG0680033159.01 | 0.917381 | 5.691379e-86 | 1.638362e-81 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680033159.01 | MsG0480021654.01 | PPI |
| MsG0680033159.01 | MsG0580025466.01 | PPI |
| MsG0680033159.01 | MsG0480021654.01 | PPI |
| MsG0680033159.01 | MsG0580025466.01 | PPI |
| MsG0680033159.01 | MsG0480021856.01 | PPI |
| MsG0680033159.01 | MsG0680035831.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033159.01.T01 | MTR_3g035130 | 97.087 | 515 | 15 | 0 | 1 | 515 | 1 | 515 | 0.0 | 1028 |
| MsG0680033159.01.T01 | MTR_3g035130 | 96.696 | 454 | 15 | 0 | 1 | 454 | 1 | 454 | 0.0 | 903 |
| MsG0680033159.01.T01 | MTR_4g057730 | 33.265 | 487 | 303 | 13 | 32 | 505 | 55 | 532 | 6.20e-61 | 209 |
| MsG0680033159.01.T01 | MTR_2g095510 | 32.091 | 483 | 311 | 11 | 32 | 505 | 55 | 529 | 5.93e-60 | 207 |
| MsG0680033159.01.T01 | MTR_4g011790 | 30.038 | 526 | 317 | 17 | 25 | 507 | 78 | 595 | 2.30e-56 | 198 |
| MsG0680033159.01.T01 | MTR_1569s0010 | 29.456 | 533 | 331 | 11 | 10 | 505 | 1 | 525 | 3.80e-56 | 196 |
| MsG0680033159.01.T01 | MTR_4g011790 | 29.981 | 517 | 315 | 16 | 25 | 502 | 78 | 586 | 1.38e-55 | 197 |
| MsG0680033159.01.T01 | MTR_5g027480 | 31.006 | 487 | 314 | 11 | 32 | 505 | 68 | 545 | 3.03e-55 | 194 |
| MsG0680033159.01.T01 | MTR_3g460770 | 27.745 | 501 | 328 | 12 | 32 | 514 | 56 | 540 | 5.34e-55 | 194 |
| MsG0680033159.01.T01 | MTR_4g011790 | 29.533 | 535 | 317 | 18 | 25 | 507 | 78 | 604 | 8.65e-54 | 191 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680033159.01.T01 | AT3G48990 | 75.147 | 511 | 123 | 3 | 6 | 514 | 5 | 513 | 0.0 | 767 |
| MsG0680033159.01.T01 | AT4G05160 | 29.143 | 525 | 349 | 12 | 5 | 514 | 27 | 543 | 1.65e-55 | 195 |
| MsG0680033159.01.T01 | AT1G20510 | 29.545 | 484 | 326 | 11 | 31 | 505 | 57 | 534 | 3.65e-55 | 194 |
| MsG0680033159.01.T01 | AT3G21240 | 29.459 | 499 | 307 | 15 | 33 | 505 | 65 | 544 | 8.73e-55 | 193 |
| MsG0680033159.01.T01 | AT3G16170 | 30.498 | 482 | 284 | 15 | 68 | 514 | 142 | 607 | 3.67e-54 | 192 |
| MsG0680033159.01.T01 | AT4G19010 | 29.592 | 490 | 323 | 11 | 30 | 505 | 71 | 552 | 7.06e-54 | 191 |
| MsG0680033159.01.T01 | AT5G38120 | 31.250 | 512 | 312 | 16 | 24 | 514 | 57 | 549 | 6.11e-53 | 188 |
| MsG0680033159.01.T01 | AT1G20480 | 28.630 | 489 | 326 | 11 | 32 | 505 | 75 | 555 | 1.67e-52 | 187 |
| MsG0680033159.01.T01 | AT1G20500 | 29.652 | 489 | 317 | 13 | 32 | 505 | 64 | 540 | 1.47e-51 | 184 |
| MsG0680033159.01.T01 | AT1G65060 | 29.796 | 490 | 315 | 13 | 33 | 505 | 77 | 554 | 2.26e-51 | 184 |
Find 179 sgRNAs with CRISPR-Local
Find 670 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACCAACCTAGAATAAC+CGG | 0.441521 | 6:-58318710 | None:intergenic |
| AAAGGGGTTGGAGGGAAACT+TGG | 0.461064 | 6:+58329359 | MsG0680033159.01.T01:three_prime_UTR |
| AACCACAAGTCGGCCTAAGG+GGG | 0.637615 | 6:+58318346 | MsG0680033159.01.T01:CDS |
| AACCGGAGTTTCGGGTAAAC+CGG | 0.388792 | 6:-58318659 | None:intergenic |
| AACCGGTTTACCCGAAACTC+CGG | 0.542049 | 6:+58318657 | MsG0680033159.01.T01:CDS |
| AACCTCGATGGTGTTGGGGA+AGG | 0.626421 | 6:-58317537 | None:intergenic |
| AACGGCGAGGTTTGTATTAA+GGG | 0.358728 | 6:+58321985 | MsG0680033159.01.T01:CDS |
| AAGATGGTTCCCACAAAGCT+GGG | 0.557458 | 6:+58321890 | MsG0680033159.01.T01:CDS |
| AAGGAGCTCATCAATAGAGG+AGG | 0.664962 | 6:+58322138 | MsG0680033159.01.T01:CDS |
| AAGGGACAAAATGTTACAAA+AGG | 0.489834 | 6:+58322003 | MsG0680033159.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACTACGGAAGTTAAGAA+TGG | - | Chr6:58319956-58319975 | None:intergenic | 30.0% | |
| AAAAGAACACTGGAAATAGT+AGG | - | Chr6:58327783-58327802 | None:intergenic | 30.0% | |
| AAACACCCTTAAGCTAAAAA+AGG | + | Chr6:58323889-58323908 | MsG0680033159.01.T01:intron | 30.0% | |
| AAATAGAAAATGGCAAGGTT+AGG | - | Chr6:58320513-58320532 | None:intergenic | 30.0% | |
| AACTTCATAGAAAAAGAGAG+AGG | - | Chr6:58318752-58318771 | None:intergenic | 30.0% | |
| AAGAGAGACTAAATTTGTGT+GGG | - | Chr6:58324354-58324373 | None:intergenic | 30.0% | |
| AAGGGACAAAATGTTACAAA+AGG | + | Chr6:58322003-58322022 | MsG0680033159.01.T01:CDS | 30.0% | |
| AAGTAAAGACTAGACTTAGA+AGG | + | Chr6:58327524-58327543 | MsG0680033159.01.T01:intron | 30.0% | |
| AAGTAACATCTGTACTGTAA+TGG | - | Chr6:58327974-58327993 | None:intergenic | 30.0% | |
| AAGTAAGGACTAAACTTACA+AGG | - | Chr6:58327081-58327100 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 58317270 | 58329514 | 58317270 | ID=MsG0680033159.01;Name=MsG0680033159.01 |
| Chr6 | mRNA | 58317270 | 58329514 | 58317270 | ID=MsG0680033159.01.T01;Parent=MsG0680033159.01;Name=MsG0680033159.01.T01;_AED=0.43;_eAED=0.43;_QI=83|1|1|1|1|1|5|199|515 |
| Chr6 | exon | 58329127 | 58329514 | 58329127 | ID=MsG0680033159.01.T01:exon:33626;Parent=MsG0680033159.01.T01 |
| Chr6 | exon | 58323563 | 58323666 | 58323563 | ID=MsG0680033159.01.T01:exon:33625;Parent=MsG0680033159.01.T01 |
| Chr6 | exon | 58321800 | 58322159 | 58321800 | ID=MsG0680033159.01.T01:exon:33624;Parent=MsG0680033159.01.T01 |
| Chr6 | exon | 58318032 | 58318722 | 58318032 | ID=MsG0680033159.01.T01:exon:33623;Parent=MsG0680033159.01.T01 |
| Chr6 | exon | 58317270 | 58317556 | 58317270 | ID=MsG0680033159.01.T01:exon:33622;Parent=MsG0680033159.01.T01 |
| Chr6 | five_prime_UTR | 58317270 | 58317352 | 58317270 | ID=MsG0680033159.01.T01:five_prime_utr;Parent=MsG0680033159.01.T01 |
| Chr6 | CDS | 58317353 | 58317556 | 58317353 | ID=MsG0680033159.01.T01:cds;Parent=MsG0680033159.01.T01 |
| Chr6 | CDS | 58318032 | 58318722 | 58318032 | ID=MsG0680033159.01.T01:cds;Parent=MsG0680033159.01.T01 |
| Chr6 | CDS | 58321800 | 58322159 | 58321800 | ID=MsG0680033159.01.T01:cds;Parent=MsG0680033159.01.T01 |
| Chr6 | CDS | 58323563 | 58323666 | 58323563 | ID=MsG0680033159.01.T01:cds;Parent=MsG0680033159.01.T01 |
| Chr6 | CDS | 58329127 | 58329315 | 58329127 | ID=MsG0680033159.01.T01:cds;Parent=MsG0680033159.01.T01 |
| Chr6 | three_prime_UTR | 58329316 | 58329514 | 58329316 | ID=MsG0680033159.01.T01:three_prime_utr;Parent=MsG0680033159.01.T01 |
| Gene Sequence |
>MsG0680033159.01.T01 ATGGAAACCGCAACCACCCTCACCGGATTACTCCAATCCGTCGCTGAAAAATTCCCCTCCCGTCGTGCCATCTCCGTCGCCGGAAAATTCGACCTCACCCATTCCCACCTCCATGAATTAGTCCAATCCGCTGCCAATCATCTCATCTCCGCTGGAATCAAACCCAACGATGTCGTCGCTCTCACCTTCCCCAACACCATCGAGTATGTTATCATGTTCCTTGCCGTGATTCGAGTCCGAGCCACGGCAGCACCATTAAACGCAGCATACACATCAGAAGAATTCGAGTTTTATCTCTCCGACTCAGAATCAAAGCTTCTCTTAACACCATTAGAAGGCAACAACGCTGCTCAAACCGCAGCTTCAAAGCTCAACATTCCACTCGGTACGGTTTCCTTCAATAAAACCGAAGAAGAAACAAAACTTTCAATCTCGCTGACCCAAACCGAGTCAGCGAACTCGGTTTCAGAACTCATTAACGAACCATCCGACGTGGCACTTTTTCTCCACACATCAGGAACCACAAGTCGGCCTAAGGGGGTCCCACTGAGTCAGCACAATTTGGTTTCATCGGTTCGAAACATTGAATCGGTTTATCGGCTCAGTGAGTTGGATTCGACTGTGATTGTTCTTCCTCTTTTTCATGTTCATGGGTTGATTGCTGGGTTGCTGAGTTCACTCGGTGCCGGAGGTGCGGTGGCGTTGCCGGCGGCGGGGAGATTCTCTGCATCAACGTTTTGGAAGGATATGATTCAATATAATGCCACGTGGTACACGGCGGTTCCTACCATACACCAGATTATATTGGACCGTCATCAAAGTAACCCTGAACCGGTTTACCCGAAACTCCGGTTTATTCGTAGCTGTAGTGCTTCGTTGGCTCCGGTTATTCTAGATCGTTTGGAGGAAGCATTTGGGGCACCCGTTTTGGAAGCTTATGCTATGACCGAGGCTACTCATTTGATGTGTTCAAATCCTTTGCCAGAAGATGGTTCCCACAAAGCTGGGTCGGTTGGGAAACCTGTGGGTCAAGAAATGGCTATTTTGGATGAATCGGGTCGGATTATGGAGGCTGATGTTAACGGCGAGGTTTGTATTAAGGGACAAAATGTTACAAAAGGCTATAAGAACAATGAAGAAGCTAATAAGTCAGCATTTTTGTTTGGTTGGTTTCATACTGGTGATATTGGTTACTTTGATTCTGATGGGTATTTGCATCTTGTGGGTCGGATTAAGGAGCTCATCAATAGAGGAGGAGAGAAAATATCACCAATTGAAGTGGATGCTGTCCTTCTGGCACATCCAGACGTAGCTCAGGCAGTTGCTTTCGGAGTGCCAGATCAGAAATACGGTGAAGAGATTCATTGTGCAATCATTCCAAGAGAAGGATCAAATATTGATGCTGAAGAGGTGCTAAAATATTGCAAGAAGAATCTCACATCTTTTAAAGTTCCCAAAAAGGTTTTCATTACCGATTCTTTGCCCAAGACAGCCACTGGCAAGATTTTACGTCGTCTTGTGGCAGAACACTTTGTCTCTCAAGCTTGA |
| Protein sequence |
>MsG0680033159.01.T01 METATTLTGLLQSVAEKFPSRRAISVAGKFDLTHSHLHELVQSAANHLISAGIKPNDVVALTFPNTIEYVIMFLAVIRVRATAAPLNAAYTSEEFEFYLSDSESKLLLTPLEGNNAAQTAASKLNIPLGTVSFNKTEEETKLSISLTQTESANSVSELINEPSDVALFLHTSGTTSRPKGVPLSQHNLVSSVRNIESVYRLSELDSTVIVLPLFHVHGLIAGLLSSLGAGGAVALPAAGRFSASTFWKDMIQYNATWYTAVPTIHQIILDRHQSNPEPVYPKLRFIRSCSASLAPVILDRLEEAFGAPVLEAYAMTEATHLMCSNPLPEDGSHKAGSVGKPVGQEMAILDESGRIMEADVNGEVCIKGQNVTKGYKNNEEANKSAFLFGWFHTGDIGYFDSDGYLHLVGRIKELINRGGEKISPIEVDAVLLAHPDVAQAVAFGVPDQKYGEEIHCAIIPREGSNIDAEEVLKYCKKNLTSFKVPKKVFITDSLPKTATGKILRRLVAEHFVSQA* |
