AlfalfaGEDB | MsG0680033673.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033673.01

KEGG: -
GO: GO:0005515
PFAM: PF01535 PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033673.01.T01	RHN51821.1	79.119	522	107	1	51	570	71	592	0	865

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033673.01.T01	Q9LQ14	35.778	559	317	2	64	587	61	612	5.02E-131	400

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033673.01.T01	A0A396HH13	79.119	522	107	1	51	570	71	592	0.0	865

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680033673.01	PPI
MsG0080048802.01	MsG0680033673.01	PPI
MsG0080048802.01	MsG0680033673.01	PPI
MsG0080048925.01	MsG0680033673.01	PPI
MsG0080048925.01	MsG0680033673.01	PPI
MsG0080048926.01	MsG0680033673.01	PPI
MsG0180000086.01	MsG0680033673.01	PPI
MsG0180001046.01	MsG0680033673.01	PPI
MsG0180001047.01	MsG0680033673.01	PPI
MsG0180001883.01	MsG0680033673.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033673.01.T01	MTR_6g445320	78.269	520	102	3	52	570	28	537	0.0	832
MsG0680033673.01.T01	MTR_0308s0020	75.530	519	125	1	54	570	23	541	0.0	825
MsG0680033673.01.T01	MTR_0177s0060	77.099	524	97	4	52	570	12	517	0.0	819
MsG0680033673.01.T01	MTR_0177s0060	28.060	335	224	1	82	399	187	521	2.08e-39	151
MsG0680033673.01.T01	MTR_3g034990	76.245	522	120	2	53	570	34	555	0.0	817
MsG0680033673.01.T01	MTR_0019s0210	75.096	522	125	2	54	570	29	550	0.0	803
MsG0680033673.01.T01	MTR_0014s0210	77.555	499	107	2	74	570	1	496	0.0	802
MsG0680033673.01.T01	MTR_0014s0210	26.492	419	303	2	48	464	80	495	2.22e-55	195
MsG0680033673.01.T01	MTR_7g012890	72.587	518	136	2	59	570	29	546	0.0	775
MsG0680033673.01.T01	MTR_0014s0180	71.977	521	142	1	54	570	28	548	0.0	773

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033673.01.T01	AT1G62930	35.778	559	317	2	64	587	61	612	5.12e-132	400
MsG0680033673.01.T01	AT1G62930	26.058	449	295	3	45	456	180	628	1.96e-48	179
MsG0680033673.01.T01	AT1G62930	31.987	297	202	0	273	569	60	356	1.15e-38	151
MsG0680033673.01.T01	AT1G62910	37.179	546	306	2	64	574	64	607	5.36e-132	400
MsG0680033673.01.T01	AT1G62910	29.067	375	231	1	231	570	56	430	6.25e-54	194
MsG0680033673.01.T01	AT1G62910	29.680	219	149	1	351	564	31	249	2.79e-24	107
MsG0680033673.01.T01	AT1G12700	38.020	505	313	0	65	569	70	574	1.39e-131	398
MsG0680033673.01.T01	AT1G12700	26.959	434	315	1	47	478	155	588	2.91e-64	222
MsG0680033673.01.T01	AT1G12700	31.818	330	225	0	241	570	71	400	5.60e-54	194
MsG0680033673.01.T01	AT1G12700	25.140	358	268	0	64	421	244	601	1.22e-39	153

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Find 117 sgRNAs with CRISPR-Local

Find 379 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATGTATTCAACATCAT+AGG	0.335413	6:-70835531	MsG0680033673.01.T01:CDS
AACATGTATTCAACATCATA+GGG	0.518579	6:-70835530	MsG0680033673.01.T01:CDS
AAGATTCCCGCCAGTTGGAA+CGG	0.512793	6:-70839261	MsG0680033673.01.T01:CDS
AAGGCTATAATTTAACTGTT+TGG	0.327400	6:-70835083	MsG0680033673.01.T01:CDS
AATAGCATTATCAACAGTAT+TGG	0.419239	6:+70836391	None:intergenic
AATCTTTCGTATCCAACTGG+CGG	0.648162	6:+70839278	None:intergenic
AATCTTTCTCAACATTTGCA+AGG	0.493291	6:+70835953	None:intergenic
AATTGAATGATGGATGGAGT+AGG	0.582857	6:+70836338	None:intergenic
AATTGGAATTCAATGAAATT+AGG	0.243491	6:-70836250	MsG0680033673.01.T01:CDS
ACACTTTCACGACCATGTGG+TGG	0.652314	6:-70836054	MsG0680033673.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATCAGTGAGGTTATCT+CGG	+	Chr6:70836670-70836689	None:intergenic	30.0%
AAAGGAGCAACTAAATCAAT+CGG	+	Chr6:70837115-70837134	None:intergenic	30.0%
AACATAATTAACCGTCTGAT+GGG	-	Chr6:70835764-70835783	MsG0680033673.01.T01:CDS	30.0%
AAGATGCATCTTGTTGATAT+GGG	+	Chr6:70834922-70834941	None:intergenic	30.0%
AATCTTTCTCAACATTTGCA+AGG	+	Chr6:70838077-70838096	None:intergenic	30.0%
ACATACAACATACTTATCGA+TGG	-	Chr6:70838862-70838881	MsG0680033673.01.T01:intron	30.0%
ACTGATAAGTATATGGGAAT+CGG	+	Chr6:70836900-70836919	None:intergenic	30.0%
ACTTACAATTCTCTCATTGA+TGG	-	Chr6:70838652-70838671	MsG0680033673.01.T01:intron	30.0%
ATCGATAAGTATGTTGTATG+TGG	+	Chr6:70838863-70838882	None:intergenic	30.0%
ATGAAAGGTTTGTGTCTTAA+TGG	-	Chr6:70837932-70837951	MsG0680033673.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	70834713	70839336	70834713	ID=MsG0680033673.01;Name=MsG0680033673.01
Chr6	mRNA	70834713	70839336	70834713	ID=MsG0680033673.01.T01;Parent=MsG0680033673.01;Name=MsG0680033673.01.T01;_AED=0.44;_eAED=0.46;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|588
Chr6	exon	70839185	70839336	70839185	ID=MsG0680033673.01.T01:exon:10712;Parent=MsG0680033673.01.T01
Chr6	exon	70834886	70836442	70834886	ID=MsG0680033673.01.T01:exon:10713;Parent=MsG0680033673.01.T01
Chr6	exon	70834713	70834770	70834713	ID=MsG0680033673.01.T01:exon:10714;Parent=MsG0680033673.01.T01
Chr6	CDS	70839185	70839336	70839185	ID=MsG0680033673.01.T01:cds;Parent=MsG0680033673.01.T01
Chr6	CDS	70834886	70836442	70834886	ID=MsG0680033673.01.T01:cds;Parent=MsG0680033673.01.T01
Chr6	CDS	70834713	70834770	70834713	ID=MsG0680033673.01.T01:cds;Parent=MsG0680033673.01.T01

Gene Sequence

>MsG0680033673.01.T01

ATGCTCTGTAACACCCACGACGTGGCTTTCGATATCCCGCCAGTTGGATACGAAAGATTCCCGCCAGTTGGAACGGGCACTATCTGTAAGGATGACCCCATATTAATAAGAGGAACCGAATCACATCGGAATGAGAGGGATCCGGAGGCCGTAACACTACACTCTAACACTAGATTTGTTCCCAATACTGTTGATAATGCTATTTCTTCATTCAATCACATGATCGGTTTGAATCCTACTCCATCCATCATTCAATTTAACAAAATTTTAACCTCACTTGTTAAGTTGAACCATTACCCCACTGTTATTTCCCTTTCTCACAAATTGGAATTCAATGAAATTAGGCCAGATATTTTTACTTTTACCATCTTAATTAATTGCTACTGTCATGTTCATCAAATGCCTTTTGCCTTTTCTATATTGGCCAAAATTCTCAAGATGGGTTATCAGCCCGATACCATAACCTTGACTACACTTATGAAAGGTTTGTGTCTTAATGGTAAGGTCAATGAAGCTCTACACTTTCACGACCATGTGGTGGCAAAGGGATTTCAATTAGATCAAGTTAGTTATGGAACCTTGATCAATGGACTGTGCAAAATTGGGGAAACAAGAGCAGCCTTGCAAATGTTGAGAAAGATTGATGGTAAATTGGTCAAGATCAATGTGGTAATGTATAACACAATCATTGATAGTTTGAGTAAACATACATTGGTAATTGATGCCTATGAGTTATATTCTGAAATGATTGCAAAGAAAATTTCTCCTGATGTTGTCACTTTTAGTGCTCTAATTTATGGATTTTGCGTTGTTGGTCAACTGAAAGAAGCATTTGGTTTGTTCCATCAAATGGTATTGAAAAACATCAATCCAAATGTTTATACTTTTAATATATTGGTCGATGCTCTTTGTAAGGATGGAAAAGTGAAAGAAGCTAAGAATTTATTAGCTGTGATGATGAAAGAAGGTGTGGAACCTACTGTTGTTACATATAATACATTAATGGATGGGTATTGCCTAGCTAATGAAGTGAATAAGGCAAAACATGTATTCAACATCATAGGGAAAAGGAAAGCGACACCTGATGTTCACAGCTACAATATCATTATTAATGGCTTATGTAAGATTAAAATGGTCGATGAAGCCTTGAGTCTCTTTAATGAAATGTGTTGCAAAGGAATTACTCCTGATGAGGTAACTTACAATTCTCTCATTGATGGTTTGTGCAAATCAGAGAGAATCTCTCATGCATGGGATCTTCTTGATCAGATGCATGATAGAGGTCAACCTGCCAATGTAATCACTTACAGCTCCTTCTTACATGCTTTATGTAAAAATCGTCATGTTGACAAGGCAATTGAATTGGTCAAGAAGATCAAAGACCAAGGAATTCAACCAAATATGACCACATACAACATACTTATCGATGGACTATGCAAAGAAGGAAGACTTAAGAATGCACAAGTGATTTTTCAAGATCTTTTGATTAAAGGCTATAATTTAACTGTTTGGACATATAATATCATGATCAATGGTCTTTGTTTAAAGGGCTTATTTGATGAAGCTGTGACCCTGCTGTCAAAGATGGAAGACAATGGTTGCATTCCAGATGTTGTAACTTATGAAACAATTATCCGTGCTCTCTTTAAAAATGATGAGAATGATAAGGCAGAGAAACTTCTACGTGAAATGATTGCTCGAGGTCTACTATATGCAAATGATGATCAACTACACGATGATCTCACTATCACCTTAGTTTTAATTTGA

Protein sequence

>MsG0680033673.01.T01

MLCNTHDVAFDIPPVGYERFPPVGTGTICKDDPILIRGTESHRNERDPEAVTLHSNTRFVPNTVDNAISSFNHMIGLNPTPSIIQFNKILTSLVKLNHYPTVISLSHKLEFNEIRPDIFTFTILINCYCHVHQMPFAFSILAKILKMGYQPDTITLTTLMKGLCLNGKVNEALHFHDHVVAKGFQLDQVSYGTLINGLCKIGETRAALQMLRKIDGKLVKINVVMYNTIIDSLSKHTLVIDAYELYSEMIAKKISPDVVTFSALIYGFCVVGQLKEAFGLFHQMVLKNINPNVYTFNILVDALCKDGKVKEAKNLLAVMMKEGVEPTVVTYNTLMDGYCLANEVNKAKHVFNIIGKRKATPDVHSYNIIINGLCKIKMVDEALSLFNEMCCKGITPDEVTYNSLIDGLCKSERISHAWDLLDQMHDRGQPANVITYSSFLHALCKNRHVDKAIELVKKIKDQGIQPNMTTYNILIDGLCKEGRLKNAQVIFQDLLIKGYNLTVWTYNIMINGLCLKGLFDEAVTLLSKMEDNGCIPDVVTYETIIRALFKNDENDKAEKLLREMIARGLLYANDDQLHDDLTITLVLI*