AlfalfaGEDB | MsG0680033777.01

M. sativa cultivar ZhongmuNo.1 / MsG0680033777.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033777.01.T01	XP_013452083.1	77.737	539	113	1	2	540	1	532	0	858
MsG0680033777.01.T01	XP_013452083.1	31.609	348	224	3	61	403	197	535	8.66E-40	163
MsG0680033777.01.T01	XP_013452083.1	28.571	322	220	2	49	367	220	534	1.04E-33	146
MsG0680033777.01.T01	XP_013452083.1	26.667	225	165	0	318	542	72	296	2.87E-22	112
MsG0680033777.01.T01	XP_013452083.1	26.389	288	179	3	45	300	251	537	2.60E-19	103

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033777.01.T01	Q9LQ14	37.687	536	296	2	42	539	61	596	7.14E-131	398

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033777.01.T01	A0A072U9K7	77.737	539	113	1	2	540	1	532	0.0	858

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680033777.01	PPI
MsG0080048802.01	MsG0680033777.01	PPI
MsG0080048925.01	MsG0680033777.01	PPI
MsG0180001046.01	MsG0680033777.01	PPI
MsG0180001047.01	MsG0680033777.01	PPI
MsG0180001883.01	MsG0680033777.01	PPI
MsG0180001883.01	MsG0680033777.01	PPI
MsG0180001883.01	MsG0680033777.01	PPI
MsG0180001883.01	MsG0680033777.01	PPI
MsG0180001883.01	MsG0680033777.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033777.01.T01	MTR_6g445320	77.737	539	113	1	2	540	1	532	0.0	858
MsG0680033777.01.T01	MTR_6g445320	31.609	348	224	3	61	403	197	535	1.05e-43	163
MsG0680033777.01.T01	MTR_6g445320	28.571	322	220	2	49	367	220	534	1.26e-37	146
MsG0680033777.01.T01	MTR_6g445320	26.667	225	165	0	318	542	72	296	3.47e-26	112
MsG0680033777.01.T01	MTR_6g445320	26.389	288	179	3	45	300	251	537	3.15e-23	103
MsG0680033777.01.T01	MTR_0572s0010	82.470	502	74	2	42	540	43	533	0.0	842
MsG0680033777.01.T01	MTR_0572s0010	26.444	329	228	2	42	367	218	535	7.86e-36	140
MsG0680033777.01.T01	MTR_0572s0010	26.132	287	177	3	256	542	50	301	1.95e-24	107
MsG0680033777.01.T01	MTR_0572s0010	26.977	215	157	0	86	300	324	538	3.22e-24	106
MsG0680033777.01.T01	MTR_0019s0210	77.099	524	111	4	25	540	23	545	0.0	816

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680033777.01.T01	AT1G62930	37.687	536	296	2	42	539	61	596	7.28e-132	398
MsG0680033777.01.T01	AT1G62930	29.024	379	269	0	164	542	81	459	2.84e-52	188
MsG0680033777.01.T01	AT1G62930	29.396	364	222	3	211	539	58	421	1.76e-48	178
MsG0680033777.01.T01	AT1G12700	37.593	540	327	3	3	539	37	569	1.58e-131	396
MsG0680033777.01.T01	AT1G12700	27.978	361	225	1	215	540	70	430	1.45e-43	164
MsG0680033777.01.T01	AT1G12700	27.437	277	197	2	49	322	321	596	2.84e-33	134
MsG0680033777.01.T01	AT1G12700	25.822	213	158	0	331	543	81	293	7.97e-19	90.5
MsG0680033777.01.T01	AT1G12700	37.593	540	327	3	3	539	53	585	1.94e-131	396
MsG0680033777.01.T01	AT1G12700	27.978	361	225	1	215	540	86	446	1.65e-43	164
MsG0680033777.01.T01	AT1G12700	27.437	277	197	2	49	322	337	612	2.82e-33	134

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Find 89 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAATCTTGAGAATCT+TGG	0.347964	6:-73306005	None:intergenic
AAGAGAATAACAAGAAAAGT+GGG	0.510299	6:-73305707	None:intergenic
AAGGCTACAATTTAACAGTT+TGG	0.326804	6:+73307070	MsG0680033777.01.T01:CDS
AATAGCATAATCAACAGTAT+TGG	0.466023	6:-73305771	None:intergenic
AATCTTGGCGAATATAGAAA+AGG	0.509595	6:-73305990	None:intergenic
AATGCAAAATCCATAAATTA+GGG	0.370972	6:-73306368	None:intergenic
AATTGAATGATGGATGGCGT+AGG	0.554746	6:-73305824	None:intergenic
AATTTGTTTCAACATCTGCA+AGG	0.613566	6:-73306200	None:intergenic
ACATATAATATACTTATCGA+TGG	0.573220	6:+73306988	MsG0680033777.01.T01:CDS
ACATATAATATTATGATCAA+TGG	0.498176	6:+73307093	MsG0680033777.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCAATCTTGAGAATCT+TGG	-	Chr6:73306008-73306027	None:intergenic	30.0%
AAGGCTACAATTTAACAGTT+TGG	+	Chr6:73307070-73307089	MsG0680033777.01.T01:CDS	30.0%
AATCTTGGCGAATATAGAAA+AGG	-	Chr6:73305993-73306012	None:intergenic	30.0%
ACATATCTAGTTGAATGTCT+TGG	-	Chr6:73306966-73306985	None:intergenic	30.0%
ACCTACAATTCATTAATGGA+TGG	+	Chr6:73306568-73306587	MsG0680033777.01.T01:CDS	30.0%
AGAAGAAGAATAAAACCTGA+AGG	-	Chr6:73305681-73305700	None:intergenic	30.0%
ATGAAAGGTTTGTGTCTTAA+TGG	+	Chr6:73306058-73306077	MsG0680033777.01.T01:CDS	30.0%
CAGATGTTGAAACAAATTGA+AGG	+	Chr6:73306205-73306224	MsG0680033777.01.T01:CDS	30.0%
CATTTCATAAAAGAGACTCA+AGG	-	Chr6:73306728-73306747	None:intergenic	30.0%
GAAACAAATTGAAGGGAAAA+TGG	+	Chr6:73306213-73306232	MsG0680033777.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	73305656	73307287	73305656	ID=MsG0680033777.01;Name=MsG0680033777.01
Chr6	mRNA	73305656	73307287	73305656	ID=MsG0680033777.01.T01;Parent=MsG0680033777.01;Name=MsG0680033777.01.T01;_AED=0.25;_eAED=0.25;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|543
Chr6	exon	73305656	73307287	73305656	ID=MsG0680033777.01.T01:exon:3783;Parent=MsG0680033777.01.T01
Chr6	CDS	73305656	73307287	73305656	ID=MsG0680033777.01.T01:cds;Parent=MsG0680033777.01.T01

Gene Sequence

>MsG0680033777.01.T01

ATGTTGTCGCTGTTTTCATCATCCTTCAGGTTTTATTCTTCTTCTACTAAACCCACTTTTCTTGTTATTCTCTTCTTAACAAGAACACGTTACTACTCTTACTCTAGATTTGTTCCCAATACTGTTGATTATGCTATTTCTTCATTTAATCACATGCTCCGCTTGAACCCTACGCCATCCATCATTCAATTTAACAAAATTTTAACCTCCCTTCTTAAATTGAACCATTACCAAACTGTTATTTCCCTTTCTAAAAAATTGGAAATTAGGCAAGATATTTTTACTTTGAACATCTTGATTAATTGCTACTGTCATGTTCGTCAAATGCCTTTTGCCTTTTCTATATTCGCCAAGATTCTCAAGATTGGTTTTCAACCTGATACCATAACCTTCACTACACTTATGAAAGGTTTGTGTCTTAATGGTAAGGTCAACGAAGCTCTACACTTTCACGACCATGTGTTTGCACTTGGATTTCACTTGGATCATGTTAGCTATGGGACCTTGATCAATGGATTGTGTAAAATGGGAGAAACAAGACCCGCCTTGCAGATGTTGAAACAAATTGAAGGGAAAATGGTCGACACCAATGTGGTAATGTACAACACAATTATCGATAGTTTGTGTAAAGATAAACTTGTAAATGATGCATATGAGTTATATTCTGAAATGATTGCAAAGAAAATTTATCCTGATGTTGTCACTTTTAGTGCCCTAATTTATGGATTTTGCATTGTTGGTCAATTGAAAGAAGTATTTGATTTGTTTCATGAAATGGTATTGAAAAACATCAACCCTGATGTTTATACTTTTAATATATTGGTTGATGCTCTATGTAAGGAAGGAAAGATCAAAGAAGCTAAAAGTGTGATAGCTGTGATGATGAAAGAAGGTGTGATGTCAGATGTTGTTACCTACAATTCATTAATGGATGGATATTGCCTTGTTAATGAAGTTAATAAGGCCAAACATGTGTTAAGTATTATATCTCGAATGAGAGTGGCTCCCAATGCTCGTAGCTATAGTATCATGATTAATGGATTCTGTAAGATTAAAATGGTCGACAAAGCCTTGAGTCTCTTTTATGAAATGCGTTGCAGAAGAATTGCTCCTGATACGGTAACTTATAATTCTCTAATTGATGGTTTGTGCAAATCTGGGAGAATCTCCCATGCTTGGGAGCTTGTTGATGAGATGCGTGGTAATGGTCAACCAGCTGATATAATCACTTACAATTCGTTAATAGATGCTTTATGCAAAAACCATCATGTTGACAAGGCTATTGTATTAGTCAAGAAAATTAAAGACCAAGACATTCAACTAGATATGTACACATATAATATACTTATCGATGGACTATGCAAACAAGGAAGGCTTAAGGATGCACAAGTGATTTTTCAGGATCTTTTGATTAAAGGCTACAATTTAACAGTTTGGACATATAATATTATGATCAATGGTCTTTGTTTAGAGGGTTTGCTTGATGAAGCTGAGGCCTTGTTGTCAAAAATGGAAGACAATGGATGTGTTCCTGATGCTGTAACTTGTGAAACAATTATTCGCGCTCTCTTTGAAAATGATAAGAACGAAAGGGCAGAGAAACTACTACTTGAAATGATAAGAGAAGACTAG

Protein sequence

>MsG0680033777.01.T01

MLSLFSSSFRFYSSSTKPTFLVILFLTRTRYYSYSRFVPNTVDYAISSFNHMLRLNPTPSIIQFNKILTSLLKLNHYQTVISLSKKLEIRQDIFTLNILINCYCHVRQMPFAFSIFAKILKIGFQPDTITFTTLMKGLCLNGKVNEALHFHDHVFALGFHLDHVSYGTLINGLCKMGETRPALQMLKQIEGKMVDTNVVMYNTIIDSLCKDKLVNDAYELYSEMIAKKIYPDVVTFSALIYGFCIVGQLKEVFDLFHEMVLKNINPDVYTFNILVDALCKEGKIKEAKSVIAVMMKEGVMSDVVTYNSLMDGYCLVNEVNKAKHVLSIISRMRVAPNARSYSIMINGFCKIKMVDKALSLFYEMRCRRIAPDTVTYNSLIDGLCKSGRISHAWELVDEMRGNGQPADIITYNSLIDALCKNHHVDKAIVLVKKIKDQDIQLDMYTYNILIDGLCKQGRLKDAQVIFQDLLIKGYNLTVWTYNIMINGLCLEGLLDEAEALLSKMEDNGCVPDAVTCETIIRALFENDKNERAEKLLLEMIRED*