AlfalfaGEDB | MsG0680034104.01

M. sativa cultivar ZhongmuNo.1 / MsG0680034104.01

KEGG: -
GO: GO:0016491 GO:0055114
PFAM: PF13532

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034104.01.T01	XP_039682791.1	84.877	324	30	1	1	305	1	324	0	559

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034104.01.T01	P05050	32.086	187	103	5	121	304	46	211	5.29E-18	84

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180002912.01	MsG0680034104.01	0.805065	1.598049e-49	8.373956e-47
MsG0380016264.01	MsG0680034104.01	0.837551	4.861185e-57	6.336592e-54
MsG0680034104.01	MsG0780040442.01	0.834025	3.804334e-56	4.458968e-53
MsG0680034104.01	MsG0880046753.01	0.810607	1.059807e-50	6.417221e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034104.01.T01	MTR_4g029340	60.606	231	84	3	77	305	100	325	3.39e-94	282
MsG0680034104.01.T01	MTR_3g085290	33.117	154	86	4	125	277	181	318	2.28e-11	64.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680034104.01.T01	AT3G14160	62.500	248	85	4	64	305	210	455	4.31e-105	314
MsG0680034104.01.T01	AT5G01780	56.067	239	95	5	74	305	72	307	1.91e-87	264
MsG0680034104.01.T01	AT5G01780	56.067	239	95	5	74	305	72	307	1.91e-87	264
MsG0680034104.01.T01	AT5G01780	56.067	239	95	5	74	305	152	387	8.94e-87	265
MsG0680034104.01.T01	AT5G01780	56.067	239	95	5	74	305	207	442	4.60e-86	265
MsG0680034104.01.T01	AT3G14140	52.756	254	91	4	61	287	212	463	4.78e-86	266
MsG0680034104.01.T01	AT5G01780	49.660	147	65	4	74	213	207	351	1.73e-38	139

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Find 100 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGGATGTGTTAAGACC+TGG	0.549786	6:-80586532	MsG0680034104.01.T01:CDS
AAAGTAAGATTCAATCGGCC+AGG	0.525942	6:+80585640	None:intergenic
AAATTGAAAATGATGTGTCT+TGG	0.563234	6:-80586385	MsG0680034104.01.T01:CDS
AACAAAGGTGGTTTCAAACG+AGG	0.570284	6:+80586598	None:intergenic
AACAATGATTGTGACAAAGA+GGG	0.631402	6:-80586687	MsG0680034104.01.T01:intron
AACACCATGAAAGACATTTC+TGG	0.347763	6:+80585723	None:intergenic
AACACCGCTGCCCACAACAC+CGG	0.661276	6:-80587772	MsG0680034104.01.T01:intron
AACACGATCGCGACCGCGAA+TGG	0.522641	6:+80587827	None:intergenic
AACTCCAGTGGTTCCATTCG+CGG	0.497232	6:-80587840	MsG0680034104.01.T01:CDS
AAGAAAGTTCTTCTGGAATC+TGG	0.299435	6:-80585772	MsG0680034104.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAATGATTGTGACAAAGA+GGG	-	Chr6:80586639-80586658	MsG0680034104.01.T01:intron	30.0%
AATTCCAGTAAATAAGCAAG+AGG	+	Chr6:80587880-80587899	None:intergenic	30.0%
AGCAGAACCAAATTAGTAAA+GGG	-	Chr6:80586777-80586796	MsG0680034104.01.T01:intron	30.0%
ATATCCTCTTGCTTATTTAC+TGG	-	Chr6:80587873-80587892	MsG0680034104.01.T01:exon	30.0%
CGCTATTTAATACAAAGTGT+TGG	-	Chr6:80587286-80587305	MsG0680034104.01.T01:intron	30.0%
GTCTGAAAGTAAGATTCAAT+CGG	+	Chr6:80587694-80587713	None:intergenic	30.0%
GTGACTAAAACAAAGAGAAA+GGG	-	Chr6:80586265-80586284	MsG0680034104.01.T01:CDS	30.0%
TATTCGTATTTACTTGTCTG+AGG	+	Chr6:80586980-80586999	None:intergenic	30.0%
TCTCTGTTTCCTTTAAACAA+AGG	+	Chr6:80586746-80586765	None:intergenic	30.0%
TCTTGAGTAAACCACTAATT+TGG	+	Chr6:80586476-80586495	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	80585432	80587916	80585432	ID=MsG0680034104.01;Name=MsG0680034104.01
Chr6	mRNA	80585432	80587916	80585432	ID=MsG0680034104.01.T01;Parent=MsG0680034104.01;Name=MsG0680034104.01.T01;_AED=0.41;_eAED=0.41;_QI=27\|0.66\|0.5\|1\|0.66\|0.5\|4\|195\|305
Chr6	exon	80587773	80587916	80587773	ID=MsG0680034104.01.T01:exon:9618;Parent=MsG0680034104.01.T01
Chr6	exon	80586688	80586742	80586688	ID=MsG0680034104.01.T01:exon:9619;Parent=MsG0680034104.01.T01
Chr6	exon	80586165	80586631	80586165	ID=MsG0680034104.01.T01:exon:9620;Parent=MsG0680034104.01.T01
Chr6	exon	80585432	80585905	80585432	ID=MsG0680034104.01.T01:exon:9621;Parent=MsG0680034104.01.T01
Chr6	five_prime_UTR	80587890	80587916	80587890	ID=MsG0680034104.01.T01:five_prime_utr;Parent=MsG0680034104.01.T01
Chr6	CDS	80587773	80587889	80587773	ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6	CDS	80586688	80586742	80586688	ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6	CDS	80586165	80586631	80586165	ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6	CDS	80585627	80585905	80585627	ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6	three_prime_UTR	80585432	80585626	80585432	ID=MsG0680034104.01.T01:three_prime_utr;Parent=MsG0680034104.01.T01

Gene Sequence

>MsG0680034104.01.T01

ATGGACGATCGAGTTCCGCCATCACAAAACTCCAGTGGTTCCATTCGCGGTCGCGATCGTGTTCTTCCATTCAACCACCATCCTGTCTCTTCACAAACACCGCTGCCCACAACACCGGTTGCTGCTGTTGGAGATAAAACATCTATGAAGAACAATGATTGTGACAAAGAGGGTGGAAGTATTCCTCGTTTGAAACCACCTTTGTTTAAAGGAAACAGAGAAAAAAGGAAGGAGCAGAACCAAATTAGTAAAGGGGATGTGTTAAGACCTGGGATGATTCATTTGAAGGGTTACATTACATTAACCGATCAAGTTAAGATAGTGAAAGTATGTAGGGAATTAGGGTTAGGTGAAGGAGGATTTTACCAACCAAGTTATGAAGGTGGGGCTATGTTGAAATTGAAAATGATGTGTCTTGGTAGAAATTGGGATCCTCAGACAAGTAAATACGAATATCAGCGACCGTGTGATGGGTCAGTTCCCCCAAAGATTCCTGATGAATTCCTAACATTGGTTGACTCTGCAATCAAAGATTCACATTCCCTGGCCAAACACCCTAAATCCAAACTTCCCTTGATCACTCCAGACATTTGTATTGTTAATTATTACGCTAAGAACGGACAACTTGGTCTTCATCAGGACAAGGATGAAAGTGAACAGAGTATCCATGAAGGGTTGCCAGTGGTGTCATTCTCTATTGGAGACACTGCAGAGTTTTTGTATGGTGATGAAAGGGATGTTGACAAGGCAAAGAAAGTTCTTCTGGAATCTGGGGATGTTTTAATCTTTGGTGGAAAGGCCAGAAATGTCTTTCATGGTGTTAGTACTATTCAGCCAGAGACTGCTCCTAGCCGCTTGCTCGAAGAAACTAATCTCCGTAAACCTGGCCGATTGAATCTTACTTTCAGACAGTATTAA

Protein sequence

>MsG0680034104.01.T01

MDDRVPPSQNSSGSIRGRDRVLPFNHHPVSSQTPLPTTPVAAVGDKTSMKNNDCDKEGGSIPRLKPPLFKGNREKRKEQNQISKGDVLRPGMIHLKGYITLTDQVKIVKVCRELGLGEGGFYQPSYEGGAMLKLKMMCLGRNWDPQTSKYEYQRPCDGSVPPKIPDEFLTLVDSAIKDSHSLAKHPKSKLPLITPDICIVNYYAKNGQLGLHQDKDESEQSIHEGLPVVSFSIGDTAEFLYGDERDVDKAKKVLLESGDVLIFGGKARNVFHGVSTIQPETAPSRLLEETNLRKPGRLNLTFRQY*