AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034104.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034104.01.T01 MTR_4g029340 60.606 231 84 3 77 305 100 325 3.39e-94 282
MsG0680034104.01.T01 MTR_3g085290 33.117 154 86 4 125 277 181 318 2.28e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034104.01.T01 AT3G14160 62.500 248 85 4 64 305 210 455 4.31e-105 314
MsG0680034104.01.T01 AT5G01780 56.067 239 95 5 74 305 72 307 1.91e-87 264
MsG0680034104.01.T01 AT5G01780 56.067 239 95 5 74 305 72 307 1.91e-87 264
MsG0680034104.01.T01 AT5G01780 56.067 239 95 5 74 305 152 387 8.94e-87 265
MsG0680034104.01.T01 AT5G01780 56.067 239 95 5 74 305 207 442 4.60e-86 265
MsG0680034104.01.T01 AT3G14140 52.756 254 91 4 61 287 212 463 4.78e-86 266
MsG0680034104.01.T01 AT5G01780 49.660 147 65 4 74 213 207 351 1.73e-38 139

Find 100 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACATCCCCTTTACTAATT+TGG 0.146522 6:+80586542 None:intergenic
TTGAAACCACCTTTGTTTAA+AGG 0.158222 6:-80586592 MsG0680034104.01.T01:CDS
GTGAAAGTATGTAGGGAATT+AGG 0.173680 6:-80586460 MsG0680034104.01.T01:CDS
ATATCCTCTTGCTTATTTAC+TGG 0.223599 6:-80585453 MsG0680034104.01.T01:three_prime_UTR
ATCAAGGGAAGTTTGGATTT+AGG 0.229089 6:+80586226 None:intergenic
TGATGTGTCTTGGTAGAAAT+TGG 0.246815 6:-80586375 MsG0680034104.01.T01:CDS
TCAAGGGAAGTTTGGATTTA+GGG 0.256412 6:+80586227 None:intergenic
AGGAATTCATCAGGAATCTT+TGG 0.258019 6:+80586298 None:intergenic
CAAGGCAAAGAAAGTTCTTC+TGG 0.270262 6:-80585779 MsG0680034104.01.T01:CDS
CCCCATGTAATTCACAAAAT+AGG 0.275286 6:+80585560 None:intergenic
TAATTCACAAAATAGGTTTC+AGG 0.285367 6:+80585567 None:intergenic
AAGAAAGTTCTTCTGGAATC+TGG 0.299435 6:-80585772 MsG0680034104.01.T01:CDS
GGAATTCATCAGGAATCTTT+GGG 0.303780 6:+80586299 None:intergenic
ACACCACTGGCAACCCTTCA+TGG 0.331278 6:+80585857 None:intergenic
GATTCAATCGGCCAGGTTTA+CGG 0.336875 6:+80585647 None:intergenic
TGAAAGTATGTAGGGAATTA+GGG 0.346841 6:-80586459 MsG0680034104.01.T01:CDS
AACACCATGAAAGACATTTC+TGG 0.347763 6:+80585723 None:intergenic
TGGAGTGATCAAGGGAAGTT+TGG 0.352721 6:+80586219 None:intergenic
CCAGTGGTGTCATTCTCTAT+TGG 0.369089 6:-80585844 MsG0680034104.01.T01:CDS
AAGTTTGGATTTAGGGTGTT+TGG 0.370855 6:+80586234 None:intergenic
TTGGTAACTAGCATCGTAAA+TGG 0.382914 6:-80587886 MsG0680034104.01.T01:exon
TGGATTTAGGGTGTTTGGCC+AGG 0.383526 6:+80586239 None:intergenic
TTGCAGAGTCAACCAATGTT+AGG 0.385230 6:+80586278 None:intergenic
AGCAGAACCAAATTAGTAAA+GGG 0.396089 6:-80586549 MsG0680034104.01.T01:CDS
ACCTGGGATGATTCATTTGA+AGG 0.433069 6:-80586515 MsG0680034104.01.T01:CDS
TTAAGATAGTGAAAGTATGT+AGG 0.436961 6:-80586468 MsG0680034104.01.T01:CDS
ACAAATGTCTGGAGTGATCA+AGG 0.446455 6:+80586210 None:intergenic
TAATTAACAATACAAATGTC+TGG 0.460593 6:+80586199 None:intergenic
AGAAAGTTCTTCTGGAATCT+GGG 0.460884 6:-80585771 MsG0680034104.01.T01:CDS
TAAGATAGTGAAAGTATGTA+GGG 0.463631 6:-80586467 MsG0680034104.01.T01:CDS
GAGACAGGATGGTGGTTGAA+TGG 0.469387 6:+80587801 None:intergenic
ACCAATGTTAGGAATTCATC+AGG 0.473909 6:+80586289 None:intergenic
GTATGTAGGGAATTAGGGTT+AGG 0.475609 6:-80586454 MsG0680034104.01.T01:CDS
AAGGCCAGAAATGTCTTTCA+TGG 0.477207 6:-80585727 MsG0680034104.01.T01:CDS
AAGCGGCTAGGAGCAGTCTC+TGG 0.478134 6:+80585688 None:intergenic
GAATTAGGGTTAGGTGAAGG+AGG 0.478275 6:-80586445 MsG0680034104.01.T01:CDS
TTGAACGTTACCGGTGTTGT+GGG 0.486685 6:+80587762 None:intergenic
GAGCAGAACCAAATTAGTAA+AGG 0.487213 6:-80586550 MsG0680034104.01.T01:CDS
GAATATCAGCGACCGTGTGA+TGG 0.488611 6:-80586331 MsG0680034104.01.T01:CDS
ATTGAACGTTACCGGTGTTG+TGG 0.489735 6:+80587761 None:intergenic
AATCTATGATAGTCAAAATA+CGG 0.491635 6:+80585592 None:intergenic
AACTCCAGTGGTTCCATTCG+CGG 0.497232 6:-80587840 MsG0680034104.01.T01:CDS
TTTGATAAGGTTGCTGCTGT+TGG 0.497816 6:-80586729 MsG0680034104.01.T01:intron
TCCTGATGAATTCCTAACAT+TGG 0.507183 6:-80586290 MsG0680034104.01.T01:CDS
AATATCAGCGACCGTGTGAT+GGG 0.507213 6:-80586330 MsG0680034104.01.T01:CDS
TTTCACTATCTTAACTTGAT+CGG 0.509198 6:+80586477 None:intergenic
ACCAACCAAGTTATGAAGGT+GGG 0.510742 6:-80586417 MsG0680034104.01.T01:CDS
ATAGCCCCACCTTCATAACT+TGG 0.511486 6:+80586412 None:intergenic
CCTGGGATGATTCATTTGAA+GGG 0.514599 6:-80586514 MsG0680034104.01.T01:CDS
GAAACTAATCTCCGTAAACC+TGG 0.515725 6:-80585658 MsG0680034104.01.T01:CDS
AATCAAAGATTCACATTCCC+TGG 0.515806 6:-80586257 MsG0680034104.01.T01:CDS
ATTCGTATCTCGATACCCAT+TGG 0.516805 6:-80587905 None:intergenic
GGATTTAGGGTGTTTGGCCA+GGG 0.519052 6:+80586240 None:intergenic
CGGACAACTTGGTCTTCATC+AGG 0.520033 6:-80586164 MsG0680034104.01.T01:intron
GTCTGAAAGTAAGATTCAAT+CGG 0.520803 6:+80585635 None:intergenic
AACACGATCGCGACCGCGAA+TGG 0.522641 6:+80587827 None:intergenic
AAAGTAAGATTCAATCGGCC+AGG 0.525942 6:+80585640 None:intergenic
TTACGATGCTAGTTACCAAT+GGG 0.526785 6:+80587890 None:intergenic
GATGTGTCTTGGTAGAAATT+GGG 0.527686 6:-80586374 MsG0680034104.01.T01:CDS
TACGCTAAGAACGGACAACT+TGG 0.530542 6:-80586175 MsG0680034104.01.T01:CDS
GTTAATTATTACGCTAAGAA+CGG 0.531004 6:-80586184 MsG0680034104.01.T01:CDS
CTGTTTCCTTTAAACAAAGG+TGG 0.534028 6:+80586586 None:intergenic
CCCCACCTTCATAACTTGGT+TGG 0.537014 6:+80586416 None:intergenic
TCTCTGTTTCCTTTAAACAA+AGG 0.540412 6:+80586583 None:intergenic
TTTACGATGCTAGTTACCAA+TGG 0.540512 6:+80587889 None:intergenic
GTTACCGGTGTTGTGGGCAG+CGG 0.542345 6:+80587768 None:intergenic
TATTCGTATTTACTTGTCTG+AGG 0.548174 6:+80586349 None:intergenic
AAAGGGGATGTGTTAAGACC+TGG 0.549786 6:-80586532 MsG0680034104.01.T01:CDS
AGCGGTGTTTGTGAAGAGAC+AGG 0.560348 6:+80587786 None:intergenic
AAATTGAAAATGATGTGTCT+TGG 0.563234 6:-80586385 MsG0680034104.01.T01:CDS
TGAAGGATGTAAAATTAGCA+TGG 0.565950 6:+80585518 None:intergenic
AACAAAGGTGGTTTCAAACG+AGG 0.570284 6:+80586598 None:intergenic
CCCTTCAAATGAATCATCCC+AGG 0.577935 6:+80586514 None:intergenic
GCGACCGCGAATGGAACCAC+TGG 0.580510 6:+80587836 None:intergenic
GATTAGTTTCTTCGAGCAAG+CGG 0.581328 6:+80585671 None:intergenic
GAAAGTTCTTCTGGAATCTG+GGG 0.590028 6:-80585770 MsG0680034104.01.T01:CDS
CCAACCAAGTTATGAAGGTG+GGG 0.591876 6:-80586416 MsG0680034104.01.T01:CDS
TAGAAAGTCACTGTCACTGA+AGG 0.592156 6:+80585501 None:intergenic
TTTGTGAATTACATGGGGCA+GGG 0.595697 6:-80585555 MsG0680034104.01.T01:three_prime_UTR
CAAATGTCTGGAGTGATCAA+GGG 0.599205 6:+80586211 None:intergenic
AGGGAATTAGGGTTAGGTGA+AGG 0.601485 6:-80586448 MsG0680034104.01.T01:CDS
AGTGAACAGAGTATCCATGA+AGG 0.602569 6:-80585871 MsG0680034104.01.T01:CDS
AATTCCAGTAAATAAGCAAG+AGG 0.605263 6:+80585449 None:intergenic
AAGGGGATGTGTTAAGACCT+GGG 0.608973 6:-80586531 MsG0680034104.01.T01:CDS
TACCAACCAAGTTATGAAGG+TGG 0.609432 6:-80586418 MsG0680034104.01.T01:CDS
GTTTCTTCGAGCAAGCGGCT+AGG 0.612254 6:+80585676 None:intergenic
GCAGAACCAAATTAGTAAAG+GGG 0.612341 6:-80586548 MsG0680034104.01.T01:CDS
GAATTCATCAGGAATCTTTG+GGG 0.622105 6:+80586300 None:intergenic
GAACAATGATTGTGACAAAG+AGG 0.622895 6:-80586688 MsG0680034104.01.T01:CDS
GTGAACAGAGTATCCATGAA+GGG 0.626296 6:-80585870 MsG0680034104.01.T01:CDS
TTTGTGAAGAGACAGGATGG+TGG 0.630879 6:+80587793 None:intergenic
AACAATGATTGTGACAAAGA+GGG 0.631402 6:-80586687 MsG0680034104.01.T01:intron
CCAATAGAGAATGACACCAC+TGG 0.639301 6:+80585844 None:intergenic
AATTCATCAGGAATCTTTGG+GGG 0.652640 6:+80586301 None:intergenic
TGATGAAAGGGATGTTGACA+AGG 0.660233 6:-80585797 MsG0680034104.01.T01:CDS
AACACCGCTGCCCACAACAC+CGG 0.661276 6:-80587772 MsG0680034104.01.T01:intron
TATCCATGAAGGGTTGCCAG+TGG 0.666760 6:-80585860 MsG0680034104.01.T01:CDS
CCGCCATCACAAAACTCCAG+TGG 0.672392 6:-80587852 MsG0680034104.01.T01:CDS
GTGTTTGTGAAGAGACAGGA+TGG 0.696338 6:+80587790 None:intergenic
CTATGATAGTCAAAATACGG+CGG 0.710459 6:+80585595 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACAAAAATTTATTCAATTT+TGG - Chr6:80585731-80585750 MsG0680034104.01.T01:CDS 10.0%
!! ATGATTCTTTCATTAATTAA+TGG - Chr6:80586567-80586586 MsG0680034104.01.T01:CDS 15.0%
!! TTGAAATATTAATAGAGTTA+GGG - Chr6:80586418-80586437 MsG0680034104.01.T01:CDS 15.0%
!!! TAATGGATTTTATTTTGATA+AGG - Chr6:80586584-80586603 MsG0680034104.01.T01:CDS 15.0%
!!! TTTGAAATATTAATAGAGTT+AGG - Chr6:80586417-80586436 MsG0680034104.01.T01:CDS 15.0%
!! AAGATTCAAAATGTAAAATG+TGG + Chr6:80587393-80587412 None:intergenic 20.0%
!! AATCTATGATAGTCAAAATA+CGG + Chr6:80587737-80587756 None:intergenic 20.0%
!! TAATTAACAATACAAATGTC+TGG + Chr6:80587130-80587149 None:intergenic 20.0%
!!! AGGGATTAATTTATTGATTT+GGG - Chr6:80586048-80586067 MsG0680034104.01.T01:intron 20.0%
! AAATTGAAAATGATGTGTCT+TGG - Chr6:80586941-80586960 MsG0680034104.01.T01:intron 25.0%
! ATCATCACGAAATTGAAATT+AGG - Chr6:80585617-80585636 MsG0680034104.01.T01:exon 25.0%
! ATTGAATAAAAGACCCAATT+TGG + Chr6:80586095-80586114 None:intergenic 25.0%
! CTAATAAAATTGAACGTTAC+CGG + Chr6:80585576-80585595 None:intergenic 25.0%
! GTTAATTATTACGCTAAGAA+CGG - Chr6:80587142-80587161 MsG0680034104.01.T01:intron 25.0%
! GTTGAATTATTGTAAACAGA+AGG - Chr6:80585998-80586017 MsG0680034104.01.T01:intron 25.0%
! TAATTCACAAAATAGGTTTC+AGG + Chr6:80587762-80587781 None:intergenic 25.0%
! TCATCACGAAATTGAAATTA+GGG - Chr6:80585618-80585637 MsG0680034104.01.T01:exon 25.0%
! TTAAAGGAAACAGAGAAAAA+AGG - Chr6:80586750-80586769 MsG0680034104.01.T01:intron 25.0%
! TTAGTGATTACTGTATTTGT+TGG - Chr6:80585655-80585674 MsG0680034104.01.T01:CDS 25.0%
! TTGAGATTAAAAACTCAACT+GGG - Chr6:80585918-80585937 MsG0680034104.01.T01:intron 25.0%
!! AAATATTTGCCTCTATGTTT+TGG + Chr6:80586370-80586389 None:intergenic 25.0%
!! AACCTATTTTGTGAATTACA+TGG - Chr6:80587764-80587783 MsG0680034104.01.T01:intron 25.0%
!! ACCTATTTTGTGAATTACAT+GGG - Chr6:80587765-80587784 MsG0680034104.01.T01:intron 25.0%
!! GAGGGATTAATTTATTGATT+TGG - Chr6:80586047-80586066 MsG0680034104.01.T01:intron 25.0%
!! TAAGATAGTGAAAGTATGTA+GGG - Chr6:80586859-80586878 MsG0680034104.01.T01:intron 25.0%
!! TTAAGATAGTGAAAGTATGT+AGG - Chr6:80586858-80586877 MsG0680034104.01.T01:intron 25.0%
!! TTTACTCAAGAAGATTTTGT+AGG - Chr6:80586485-80586504 MsG0680034104.01.T01:CDS 25.0%
!! TTTCACTATCTTAACTTGAT+CGG + Chr6:80586852-80586871 None:intergenic 25.0%
!! TTTGAGATTAAAAACTCAAC+TGG - Chr6:80585917-80585936 MsG0680034104.01.T01:intron 25.0%
!!! AAAATTTTAAGGGACCAAAT+TGG - Chr6:80586078-80586097 MsG0680034104.01.T01:intron 25.0%
!!! AAATTTTAAGGGACCAAATT+GGG - Chr6:80586079-80586098 MsG0680034104.01.T01:intron 25.0%
AACAATGATTGTGACAAAGA+GGG - Chr6:80586639-80586658 MsG0680034104.01.T01:intron 30.0%
AATTCCAGTAAATAAGCAAG+AGG + Chr6:80587880-80587899 None:intergenic 30.0%
AGCAGAACCAAATTAGTAAA+GGG - Chr6:80586777-80586796 MsG0680034104.01.T01:intron 30.0%
ATATCCTCTTGCTTATTTAC+TGG - Chr6:80587873-80587892 MsG0680034104.01.T01:exon 30.0%
CGCTATTTAATACAAAGTGT+TGG - Chr6:80587286-80587305 MsG0680034104.01.T01:intron 30.0%
GTCTGAAAGTAAGATTCAAT+CGG + Chr6:80587694-80587713 None:intergenic 30.0%
GTGACTAAAACAAAGAGAAA+GGG - Chr6:80586265-80586284 MsG0680034104.01.T01:CDS 30.0%
TATTCGTATTTACTTGTCTG+AGG + Chr6:80586980-80586999 None:intergenic 30.0%
TCTCTGTTTCCTTTAAACAA+AGG + Chr6:80586746-80586765 None:intergenic 30.0%
TCTTGAGTAAACCACTAATT+TGG + Chr6:80586476-80586495 None:intergenic 30.0%
TGAAGGATGTAAAATTAGCA+TGG + Chr6:80587811-80587830 None:intergenic 30.0%
TGTGACTAAAACAAAGAGAA+AGG - Chr6:80586264-80586283 MsG0680034104.01.T01:CDS 30.0%
TTACAAAGACAGAACCAATT+TGG + Chr6:80586180-80586199 None:intergenic 30.0%
! ATTTTCCTCGGTTTCAATTT+CGG + Chr6:80585795-80585814 None:intergenic 30.0%
! CCTATTTTGTGAATTACATG+GGG - Chr6:80587766-80587785 MsG0680034104.01.T01:intron 30.0%
! TGAAAGTATGTAGGGAATTA+GGG - Chr6:80586867-80586886 MsG0680034104.01.T01:intron 30.0%
! TTGAAACCACCTTTGTTTAA+AGG - Chr6:80586734-80586753 MsG0680034104.01.T01:intron 30.0%
! TTTTACCAACCAAGTTATGA+AGG - Chr6:80586905-80586924 MsG0680034104.01.T01:intron 30.0%
!! AGTTTTTGTATGGTGATGAA+AGG - Chr6:80587516-80587535 MsG0680034104.01.T01:intron 30.0%
!! ATACTACTTCTAAAACCACT+TGG + Chr6:80585894-80585913 None:intergenic 30.0%
!! GTTTTTGTATGGTGATGAAA+GGG - Chr6:80587517-80587536 MsG0680034104.01.T01:intron 30.0%
!! TACTACTTCTAAAACCACTT+GGG + Chr6:80585893-80585912 None:intergenic 30.0%
!!! ATGTTTTGGTCACATAACAA+GGG + Chr6:80586356-80586375 None:intergenic 30.0%
!!! TATGTTTTGGTCACATAACA+AGG + Chr6:80586357-80586376 None:intergenic 30.0%
!!! TGTTTTAATCTTTGGTGGAA+AGG - Chr6:80587580-80587599 MsG0680034104.01.T01:intron 30.0%
AACACCATGAAAGACATTTC+TGG + Chr6:80587606-80587625 None:intergenic 35.0%
AACATTAACACAGACACTCA+TGG + Chr6:80585981-80586000 None:intergenic 35.0%
AATCAAAGATTCACATTCCC+TGG - Chr6:80587069-80587088 MsG0680034104.01.T01:intron 35.0%
AATTCATCAGGAATCTTTGG+GGG + Chr6:80587028-80587047 None:intergenic 35.0%
ACACATCCCCTTTACTAATT+TGG + Chr6:80586787-80586806 None:intergenic 35.0%
ACATTAACACAGACACTCAT+GGG + Chr6:80585980-80585999 None:intergenic 35.0%
ACCAATGTTAGGAATTCATC+AGG + Chr6:80587040-80587059 None:intergenic 35.0%
AGGAAACAGAGAAAAAAGGA+AGG - Chr6:80586754-80586773 MsG0680034104.01.T01:intron 35.0%
AGGAATTCATCAGGAATCTT+TGG + Chr6:80587031-80587050 None:intergenic 35.0%
ATCAAGGGAAGTTTGGATTT+AGG + Chr6:80587103-80587122 None:intergenic 35.0%
CCCCATGTAATTCACAAAAT+AGG + Chr6:80587769-80587788 None:intergenic 35.0%
CTATGATAGTCAAAATACGG+CGG + Chr6:80587734-80587753 None:intergenic 35.0%
CTGTTTCCTTTAAACAAAGG+TGG + Chr6:80586743-80586762 None:intergenic 35.0%
GAACAATGATTGTGACAAAG+AGG - Chr6:80586638-80586657 MsG0680034104.01.T01:intron 35.0%
GAATTCATCAGGAATCTTTG+GGG + Chr6:80587029-80587048 None:intergenic 35.0%
GAGCAGAACCAAATTAGTAA+AGG - Chr6:80586776-80586795 MsG0680034104.01.T01:intron 35.0%
GATGTGTCTTGGTAGAAATT+GGG - Chr6:80586952-80586971 MsG0680034104.01.T01:intron 35.0%
GCAGAACCAAATTAGTAAAG+GGG - Chr6:80586778-80586797 MsG0680034104.01.T01:intron 35.0%
GGAATTCATCAGGAATCTTT+GGG + Chr6:80587030-80587049 None:intergenic 35.0%
GTTATGTGACCAAAACATAG+AGG - Chr6:80586358-80586377 MsG0680034104.01.T01:CDS 35.0%
TCAAGGGAAGTTTGGATTTA+GGG + Chr6:80587102-80587121 None:intergenic 35.0%
TCCTGATGAATTCCTAACAT+TGG - Chr6:80587036-80587055 MsG0680034104.01.T01:intron 35.0%
TGATGTGTCTTGGTAGAAAT+TGG - Chr6:80586951-80586970 MsG0680034104.01.T01:intron 35.0%
TTACGATGCTAGTTACCAAT+GGG + Chr6:80585439-80585458 None:intergenic 35.0%
TTTACGATGCTAGTTACCAA+TGG + Chr6:80585440-80585459 None:intergenic 35.0%
! AAGTTTGGATTTAGGGTGTT+TGG + Chr6:80587095-80587114 None:intergenic 35.0%
! GGGCTGTGAGAAAATTTTAA+GGG - Chr6:80586068-80586087 MsG0680034104.01.T01:intron 35.0%
! GTGAAAGTATGTAGGGAATT+AGG - Chr6:80586866-80586885 MsG0680034104.01.T01:intron 35.0%
! TGGGCTGTGAGAAAATTTTA+AGG - Chr6:80586067-80586086 MsG0680034104.01.T01:intron 35.0%
! TTGGTAACTAGCATCGTAAA+TGG - Chr6:80585440-80585459 MsG0680034104.01.T01:three_prime_UTR 35.0%
! TTTGGGGTGCAAATATCAAA+AGG + Chr6:80586693-80586712 None:intergenic 35.0%
!! AAGAAAGTTCTTCTGGAATC+TGG - Chr6:80587554-80587573 MsG0680034104.01.T01:intron 35.0%
!! AGAAAGTTCTTCTGGAATCT+GGG - Chr6:80587555-80587574 MsG0680034104.01.T01:intron 35.0%
!! TTTTGAGTAAGCCACCAAAT+TGG - Chr6:80586163-80586182 MsG0680034104.01.T01:intron 35.0%
!!! TCTGGGGATGTTTTAATCTT+TGG - Chr6:80587572-80587591 MsG0680034104.01.T01:intron 35.0%
!!! TTGTTTTAGTCACACGATGA+GGG + Chr6:80586258-80586277 None:intergenic 35.0%
!!! TTTGTTTTAGTCACACGATG+AGG + Chr6:80586259-80586278 None:intergenic 35.0%
AAAGACAGGGACCAAATTAG+TGG - Chr6:80586462-80586481 MsG0680034104.01.T01:CDS 40.0%
AAAGTAAGATTCAATCGGCC+AGG + Chr6:80587689-80587708 None:intergenic 40.0%
AACAAAGGTGGTTTCAAACG+AGG + Chr6:80586731-80586750 None:intergenic 40.0%
AAGGCCAGAAATGTCTTTCA+TGG - Chr6:80587599-80587618 MsG0680034104.01.T01:intron 40.0%
AATCCCTCACATGTTCATAC+TGG + Chr6:80586035-80586054 None:intergenic 40.0%
ACCAACCAAGTTATGAAGGT+GGG - Chr6:80586909-80586928 MsG0680034104.01.T01:intron 40.0%
ACCTGGGATGATTCATTTGA+AGG - Chr6:80586811-80586830 MsG0680034104.01.T01:intron 40.0%
AGAGACCTTGAGATATGCTT+CGG - Chr6:80585821-80585840 MsG0680034104.01.T01:CDS 40.0%
AGGAATACTTCCACCTTGTT+TGG + Chr6:80586711-80586730 None:intergenic 40.0%
AGTCCAGTATGAACATGTGA+GGG - Chr6:80586029-80586048 MsG0680034104.01.T01:intron 40.0%
ATTAACACAGACACTCATGG+GGG + Chr6:80585978-80585997 None:intergenic 40.0%
ATTTGGTCCCTGTCTTTGTA+AGG + Chr6:80586459-80586478 None:intergenic 40.0%
CAAAGACAGAACCAATTTGG+TGG + Chr6:80586177-80586196 None:intergenic 40.0%
CAAGGCAAAGAAAGTTCTTC+TGG - Chr6:80587547-80587566 MsG0680034104.01.T01:intron 40.0%
CATTAACACAGACACTCATG+GGG + Chr6:80585979-80585998 None:intergenic 40.0%
CCTGGGATGATTCATTTGAA+GGG - Chr6:80586812-80586831 MsG0680034104.01.T01:intron 40.0%
GAAACTAATCTCCGTAAACC+TGG - Chr6:80587668-80587687 MsG0680034104.01.T01:intron 40.0%
GATAGTGCCTTACAAAGACA+GGG - Chr6:80586449-80586468 MsG0680034104.01.T01:CDS 40.0%
GGTCCCTAACAATTTGATTG+CGG - Chr6:80585939-80585958 MsG0680034104.01.T01:intron 40.0%
GTATGTAGGGAATTAGGGTT+AGG - Chr6:80586872-80586891 MsG0680034104.01.T01:intron 40.0%
TACCAACCAAGTTATGAAGG+TGG - Chr6:80586908-80586927 MsG0680034104.01.T01:intron 40.0%
TAGAAAGTCACTGTCACTGA+AGG + Chr6:80587828-80587847 None:intergenic 40.0%
TAGTCCAGTATGAACATGTG+AGG - Chr6:80586028-80586047 MsG0680034104.01.T01:intron 40.0%
TGATATTTGCACCCCAAACA+AGG - Chr6:80586695-80586714 MsG0680034104.01.T01:CDS 40.0%
TGATGAAAGGGATGTTGACA+AGG - Chr6:80587529-80587548 MsG0680034104.01.T01:intron 40.0%
TTGCAGAGTCAACCAATGTT+AGG + Chr6:80587051-80587070 None:intergenic 40.0%
TTTGATAAGGTTGCTGCTGT+TGG - Chr6:80586597-80586616 MsG0680034104.01.T01:CDS 40.0%
TTTGTGAATTACATGGGGCA+GGG - Chr6:80587771-80587790 MsG0680034104.01.T01:intron 40.0%
! ACAAATGTCTGGAGTGATCA+AGG + Chr6:80587119-80587138 None:intergenic 40.0%
! AGTGAACAGAGTATCCATGA+AGG - Chr6:80587455-80587474 MsG0680034104.01.T01:intron 40.0%
! CAAATGTCTGGAGTGATCAA+GGG + Chr6:80587118-80587137 None:intergenic 40.0%
! GAATACTTCCACCTTGTTTG+GGG + Chr6:80586709-80586728 None:intergenic 40.0%
! GACACTGCAGAGTTTTTGTA+TGG - Chr6:80587506-80587525 MsG0680034104.01.T01:intron 40.0%
! GGAATACTTCCACCTTGTTT+GGG + Chr6:80586710-80586729 None:intergenic 40.0%
! GTGAACAGAGTATCCATGAA+GGG - Chr6:80587456-80587475 MsG0680034104.01.T01:intron 40.0%
! TGATAGTGCCTTACAAAGAC+AGG - Chr6:80586448-80586467 MsG0680034104.01.T01:CDS 40.0%
! TTTTGTGAATTACATGGGGC+AGG - Chr6:80587770-80587789 MsG0680034104.01.T01:intron 40.0%
!! ACTTCTAAAACCACTTGGGT+TGG + Chr6:80585889-80585908 None:intergenic 40.0%
!! CTTCTAAAACCACTTGGGTT+GGG + Chr6:80585888-80585907 None:intergenic 40.0%
!! GAAAGTTCTTCTGGAATCTG+GGG - Chr6:80587556-80587575 MsG0680034104.01.T01:intron 40.0%
!! GATTAGTTTCTTCGAGCAAG+CGG + Chr6:80587658-80587677 None:intergenic 40.0%
!! TTCAATTTCGGTGGGTTAGT+TGG + Chr6:80585783-80585802 None:intergenic 40.0%
!!! GGGGATGTTTTAATCTTTGG+TGG - Chr6:80587575-80587594 MsG0680034104.01.T01:intron 40.0%
AAAGGGGATGTGTTAAGACC+TGG - Chr6:80586794-80586813 MsG0680034104.01.T01:intron 45.0%
AAGGGGATGTGTTAAGACCT+GGG - Chr6:80586795-80586814 MsG0680034104.01.T01:intron 45.0%
AATATCAGCGACCGTGTGAT+GGG - Chr6:80586996-80587015 MsG0680034104.01.T01:intron 45.0%
ACCGCCGCAATCAAATTGTT+AGG + Chr6:80585946-80585965 None:intergenic 45.0%
AGGGAATTAGGGTTAGGTGA+AGG - Chr6:80586878-80586897 MsG0680034104.01.T01:intron 45.0%
ATAGCCCCACCTTCATAACT+TGG + Chr6:80586917-80586936 None:intergenic 45.0%
CCAACCAAGTTATGAAGGTG+GGG - Chr6:80586910-80586929 MsG0680034104.01.T01:intron 45.0%
CCAATAGAGAATGACACCAC+TGG + Chr6:80587485-80587504 None:intergenic 45.0%
CCCTAACAATTTGATTGCGG+CGG - Chr6:80585942-80585961 MsG0680034104.01.T01:intron 45.0%
CCCTTCAAATGAATCATCCC+AGG + Chr6:80586815-80586834 None:intergenic 45.0%
CCGCCGCAATCAAATTGTTA+GGG + Chr6:80585945-80585964 None:intergenic 45.0%
GAAGCATATCTCAAGGTCTC+TGG + Chr6:80585822-80585841 None:intergenic 45.0%
GAATTAGGGTTAGGTGAAGG+AGG - Chr6:80586881-80586900 MsG0680034104.01.T01:intron 45.0%
GATTCAATCGGCCAGGTTTA+CGG + Chr6:80587682-80587701 None:intergenic 45.0%
GTGTTTGTGAAGAGACAGGA+TGG + Chr6:80585539-80585558 None:intergenic 45.0%
TACGCTAAGAACGGACAACT+TGG - Chr6:80587151-80587170 MsG0680034104.01.T01:intron 45.0%
TATTTGCACCCCAAACAAGG+TGG - Chr6:80586698-80586717 MsG0680034104.01.T01:CDS 45.0%
TCCTCGGTTTCAATTTCGGT+GGG + Chr6:80585791-80585810 None:intergenic 45.0%
TGGAGTGATCAAGGGAAGTT+TGG + Chr6:80587110-80587129 None:intergenic 45.0%
TGTGGATGCAGTGTTGTCAA+TGG + Chr6:80587375-80587394 None:intergenic 45.0%
TTCCTCGGTTTCAATTTCGG+TGG + Chr6:80585792-80585811 None:intergenic 45.0%
TTTGTGAAGAGACAGGATGG+TGG + Chr6:80585536-80585555 None:intergenic 45.0%
! ATTGAACGTTACCGGTGTTG+TGG + Chr6:80585568-80585587 None:intergenic 45.0%
! GATAGAGTTTGCTTGCTTGC+AGG - Chr6:80587421-80587440 MsG0680034104.01.T01:intron 45.0%
!! CCAGTGGTGTCATTCTCTAT+TGG - Chr6:80587482-80587501 MsG0680034104.01.T01:intron 45.0%
!! GAACCACTGGAGTTTTGTGA+TGG + Chr6:80585480-80585499 None:intergenic 45.0%
!! GAAGTGTTGGTTTGAGACCT+TGG + Chr6:80585857-80585876 None:intergenic 45.0%
!! GTCTCTGGTTCGATTTTCCT+CGG + Chr6:80585807-80585826 None:intergenic 45.0%
!! TAAAACCACTTGGGTTGGGT+TGG + Chr6:80585884-80585903 None:intergenic 45.0%
!! TTGAACGTTACCGGTGTTGT+GGG + Chr6:80585567-80585586 None:intergenic 45.0%
!!! TTTTAAATAAAAGATAATTT+AGG - Chr6:80586124-80586143 MsG0680034104.01.T01:intron 5.0%
AACTCCAGTGGTTCCATTCG+CGG - Chr6:80585486-80585505 MsG0680034104.01.T01:three_prime_UTR 50.0%
ACCCACCGAAATTGAAACCG+AGG - Chr6:80585787-80585806 MsG0680034104.01.T01:CDS 50.0%
AGCGGTGTTTGTGAAGAGAC+AGG + Chr6:80585543-80585562 None:intergenic 50.0%
AGCTGCCGAAGCATATCTCA+AGG + Chr6:80585829-80585848 None:intergenic 50.0%
CCCCACCTTCATAACTTGGT+TGG + Chr6:80586913-80586932 None:intergenic 50.0%
GAATATCAGCGACCGTGTGA+TGG - Chr6:80586995-80587014 MsG0680034104.01.T01:intron 50.0%
GCTATAGCGCTGTAGCATAG+CGG - Chr6:80587320-80587339 MsG0680034104.01.T01:intron 50.0%
GTTTGCTTGCTTGCAGGACA+AGG - Chr6:80587427-80587446 MsG0680034104.01.T01:intron 50.0%
TATCCATGAAGGGTTGCCAG+TGG - Chr6:80587466-80587485 MsG0680034104.01.T01:intron 50.0%
! CGGACAACTTGGTCTTCATC+AGG - Chr6:80587162-80587181 MsG0680034104.01.T01:intron 50.0%
! GAGACAGGATGGTGGTTGAA+TGG + Chr6:80585528-80585547 None:intergenic 50.0%
! GGATTTAGGGTGTTTGGCCA+GGG + Chr6:80587089-80587108 None:intergenic 50.0%
! TGGATTTAGGGTGTTTGGCC+AGG + Chr6:80587090-80587109 None:intergenic 50.0%
!! CCACTGGAGTTTTGTGATGG+CGG + Chr6:80585477-80585496 None:intergenic 50.0%
!! TTGGGTTGGTCTGGAAGTGT+TGG + Chr6:80585870-80585889 None:intergenic 50.0%
ACACCACTGGCAACCCTTCA+TGG + Chr6:80587472-80587491 None:intergenic 55.0%
CCGCCATCACAAAACTCCAG+TGG - Chr6:80585474-80585493 MsG0680034104.01.T01:three_prime_UTR 55.0%
GTTTCTTCGAGCAAGCGGCT+AGG + Chr6:80587653-80587672 None:intergenic 55.0%
TGCCACGTGCTATAGCACTC+TGG - Chr6:80587224-80587243 MsG0680034104.01.T01:intron 55.0%
AACACCGCTGCCCACAACAC+CGG - Chr6:80585554-80585573 MsG0680034104.01.T01:three_prime_UTR 60.0%
AACACGATCGCGACCGCGAA+TGG + Chr6:80585502-80585521 None:intergenic 60.0%
AAGCGGCTAGGAGCAGTCTC+TGG + Chr6:80587641-80587660 None:intergenic 60.0%
CCAGACCAACCCAACCCAAG+TGG - Chr6:80585876-80585895 MsG0680034104.01.T01:CDS 60.0%
GGGGGAACTGACCCATCACA+CGG + Chr6:80587010-80587029 None:intergenic 60.0%
! CGCCAGAGTGCTATAGCACG+TGG + Chr6:80587229-80587248 None:intergenic 60.0%
!! CCACTTGGGTTGGGTTGGTC+TGG + Chr6:80585879-80585898 None:intergenic 60.0%
!! GTTACCGGTGTTGTGGGCAG+CGG + Chr6:80585561-80585580 None:intergenic 60.0%
GCGACCGCGAATGGAACCAC+TGG + Chr6:80585493-80585512 None:intergenic 65.0%
GCTTCGGCAGCTTCGCTCCA+AGG - Chr6:80585837-80585856 MsG0680034104.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 80585432 80587916 80585432 ID=MsG0680034104.01;Name=MsG0680034104.01
Chr6 mRNA 80585432 80587916 80585432 ID=MsG0680034104.01.T01;Parent=MsG0680034104.01;Name=MsG0680034104.01.T01;_AED=0.41;_eAED=0.41;_QI=27|0.66|0.5|1|0.66|0.5|4|195|305
Chr6 exon 80587773 80587916 80587773 ID=MsG0680034104.01.T01:exon:9618;Parent=MsG0680034104.01.T01
Chr6 exon 80586688 80586742 80586688 ID=MsG0680034104.01.T01:exon:9619;Parent=MsG0680034104.01.T01
Chr6 exon 80586165 80586631 80586165 ID=MsG0680034104.01.T01:exon:9620;Parent=MsG0680034104.01.T01
Chr6 exon 80585432 80585905 80585432 ID=MsG0680034104.01.T01:exon:9621;Parent=MsG0680034104.01.T01
Chr6 five_prime_UTR 80587890 80587916 80587890 ID=MsG0680034104.01.T01:five_prime_utr;Parent=MsG0680034104.01.T01
Chr6 CDS 80587773 80587889 80587773 ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6 CDS 80586688 80586742 80586688 ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6 CDS 80586165 80586631 80586165 ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6 CDS 80585627 80585905 80585627 ID=MsG0680034104.01.T01:cds;Parent=MsG0680034104.01.T01
Chr6 three_prime_UTR 80585432 80585626 80585432 ID=MsG0680034104.01.T01:three_prime_utr;Parent=MsG0680034104.01.T01
Gene Sequence

>MsG0680034104.01.T01

ATGGACGATCGAGTTCCGCCATCACAAAACTCCAGTGGTTCCATTCGCGGTCGCGATCGTGTTCTTCCATTCAACCACCATCCTGTCTCTTCACAAACACCGCTGCCCACAACACCGGTTGCTGCTGTTGGAGATAAAACATCTATGAAGAACAATGATTGTGACAAAGAGGGTGGAAGTATTCCTCGTTTGAAACCACCTTTGTTTAAAGGAAACAGAGAAAAAAGGAAGGAGCAGAACCAAATTAGTAAAGGGGATGTGTTAAGACCTGGGATGATTCATTTGAAGGGTTACATTACATTAACCGATCAAGTTAAGATAGTGAAAGTATGTAGGGAATTAGGGTTAGGTGAAGGAGGATTTTACCAACCAAGTTATGAAGGTGGGGCTATGTTGAAATTGAAAATGATGTGTCTTGGTAGAAATTGGGATCCTCAGACAAGTAAATACGAATATCAGCGACCGTGTGATGGGTCAGTTCCCCCAAAGATTCCTGATGAATTCCTAACATTGGTTGACTCTGCAATCAAAGATTCACATTCCCTGGCCAAACACCCTAAATCCAAACTTCCCTTGATCACTCCAGACATTTGTATTGTTAATTATTACGCTAAGAACGGACAACTTGGTCTTCATCAGGACAAGGATGAAAGTGAACAGAGTATCCATGAAGGGTTGCCAGTGGTGTCATTCTCTATTGGAGACACTGCAGAGTTTTTGTATGGTGATGAAAGGGATGTTGACAAGGCAAAGAAAGTTCTTCTGGAATCTGGGGATGTTTTAATCTTTGGTGGAAAGGCCAGAAATGTCTTTCATGGTGTTAGTACTATTCAGCCAGAGACTGCTCCTAGCCGCTTGCTCGAAGAAACTAATCTCCGTAAACCTGGCCGATTGAATCTTACTTTCAGACAGTATTAA
Protein sequence

>MsG0680034104.01.T01

MDDRVPPSQNSSGSIRGRDRVLPFNHHPVSSQTPLPTTPVAAVGDKTSMKNNDCDKEGGSIPRLKPPLFKGNREKRKEQNQISKGDVLRPGMIHLKGYITLTDQVKIVKVCRELGLGEGGFYQPSYEGGAMLKLKMMCLGRNWDPQTSKYEYQRPCDGSVPPKIPDEFLTLVDSAIKDSHSLAKHPKSKLPLITPDICIVNYYAKNGQLGLHQDKDESEQSIHEGLPVVSFSIGDTAEFLYGDERDVDKAKKVLLESGDVLIFGGKARNVFHGVSTIQPETAPSRLLEETNLRKPGRLNLTFRQY*