Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034383.01.T01 | XP_003619736.1 | 91.707 | 205 | 17 | 0 | 1 | 205 | 1 | 205 | 7.88E-135 | 393 |
| MsG0680034383.01.T01 | XP_003619736.1 | 93.478 | 46 | 3 | 0 | 206 | 251 | 295 | 340 | 1.10E-18 | 95.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034383.01.T01 | Q9LT10 | 49 | 200 | 87 | 3 | 10 | 205 | 13 | 201 | 1.06E-68 | 218 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034383.01.T01 | G7KP79 | 91.707 | 205 | 17 | 0 | 1 | 205 | 1 | 205 | 3.76e-135 | 393 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000976.01 | MsG0680034383.01 | 0.808702 | 2.719480e-50 | 1.565663e-47 |
| MsG0180004058.01 | MsG0680034383.01 | -0.800430 | 1.446468e-48 | 6.733980e-46 |
| MsG0180004064.01 | MsG0680034383.01 | -0.808214 | 3.457658e-50 | 1.965445e-47 |
| MsG0680034383.01 | MsG0780037999.01 | -0.817137 | 3.854252e-52 | 2.783826e-49 |
| MsG0680034383.01 | MsG0780039873.01 | 0.825740 | 3.974667e-54 | 3.654266e-51 |
| MsG0680034383.01 | MsG0880044834.01 | 0.800467 | 1.421897e-48 | 6.625318e-46 |
| MsG0680034383.01 | MsG0880047090.01 | -0.801055 | 1.078527e-48 | 5.101431e-46 |
| MsG0480020928.01 | MsG0680034383.01 | -0.801547 | 8.551041e-49 | 4.094847e-46 |
| MsG0480018338.01 | MsG0680034383.01 | 0.819757 | 9.822768e-53 | 7.626685e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034383.01.T01 | MTR_6g065370 | 91.707 | 205 | 17 | 0 | 1 | 205 | 1 | 205 | 9.54e-139 | 393 |
| MsG0680034383.01.T01 | MTR_6g065370 | 93.478 | 46 | 3 | 0 | 206 | 251 | 295 | 340 | 1.33e-22 | 95.1 |
| MsG0680034383.01.T01 | MTR_6g065400 | 90.732 | 205 | 19 | 0 | 1 | 205 | 1 | 205 | 8.19e-137 | 389 |
| MsG0680034383.01.T01 | MTR_6g065400 | 93.478 | 46 | 3 | 0 | 206 | 251 | 295 | 340 | 1.34e-22 | 95.1 |
| MsG0680034383.01.T01 | MTR_6g065270 | 78.713 | 202 | 39 | 1 | 8 | 205 | 6 | 207 | 8.72e-116 | 335 |
| MsG0680034383.01.T01 | MTR_6g065270 | 82.609 | 46 | 8 | 0 | 206 | 251 | 297 | 342 | 8.16e-20 | 87.4 |
| MsG0680034383.01.T01 | MTR_6g065340 | 80.882 | 204 | 39 | 0 | 2 | 205 | 13 | 216 | 3.06e-112 | 326 |
| MsG0680034383.01.T01 | MTR_6g065340 | 78.261 | 46 | 10 | 0 | 206 | 251 | 290 | 335 | 6.47e-19 | 84.7 |
| MsG0680034383.01.T01 | MTR_6g065120 | 75.122 | 205 | 50 | 1 | 2 | 205 | 3 | 207 | 7.95e-109 | 318 |
| MsG0680034383.01.T01 | MTR_6g065120 | 78.261 | 46 | 10 | 0 | 206 | 251 | 297 | 342 | 6.92e-19 | 84.7 |
| MsG0680034383.01.T01 | MTR_6g065110 | 68.159 | 201 | 64 | 0 | 5 | 205 | 6 | 206 | 8.95e-100 | 295 |
| MsG0680034383.01.T01 | MTR_6g065110 | 63.043 | 46 | 16 | 1 | 206 | 250 | 296 | 341 | 3.53e-13 | 68.6 |
| MsG0680034383.01.T01 | MTR_6g065430 | 55.721 | 201 | 80 | 2 | 7 | 205 | 6 | 199 | 3.49e-73 | 226 |
| MsG0680034383.01.T01 | MTR_1g082210 | 37.931 | 203 | 120 | 2 | 1 | 198 | 7 | 208 | 1.03e-39 | 140 |
| MsG0680034383.01.T01 | MTR_1g089310 | 38.095 | 210 | 121 | 3 | 1 | 205 | 7 | 212 | 1.97e-39 | 140 |
| MsG0680034383.01.T01 | MTR_1g089310 | 38.095 | 210 | 121 | 3 | 1 | 205 | 23 | 228 | 2.44e-39 | 140 |
| MsG0680034383.01.T01 | MTR_7g093950 | 35.071 | 211 | 130 | 2 | 1 | 205 | 7 | 216 | 5.70e-37 | 133 |
| MsG0680034383.01.T01 | MTR_6g464280 | 38.418 | 177 | 99 | 4 | 32 | 205 | 24 | 193 | 1.30e-33 | 124 |
| MsG0680034383.01.T01 | MTR_8g035540 | 37.349 | 166 | 90 | 2 | 47 | 205 | 30 | 188 | 2.69e-31 | 118 |
| MsG0680034383.01.T01 | MTR_6g464510 | 37.079 | 178 | 101 | 4 | 31 | 205 | 27 | 196 | 2.40e-29 | 112 |
| MsG0680034383.01.T01 | MTR_0007s0520 | 37.056 | 197 | 105 | 5 | 1 | 196 | 1 | 179 | 3.31e-29 | 112 |
| MsG0680034383.01.T01 | MTR_6g464320 | 38.095 | 168 | 90 | 4 | 32 | 195 | 25 | 182 | 1.21e-28 | 111 |
| MsG0680034383.01.T01 | MTR_6g464120 | 37.333 | 150 | 85 | 3 | 49 | 196 | 43 | 185 | 1.63e-28 | 113 |
| MsG0680034383.01.T01 | MTR_1g102160 | 34.911 | 169 | 88 | 4 | 31 | 192 | 23 | 176 | 9.51e-28 | 108 |
| MsG0680034383.01.T01 | MTR_1g102170 | 34.706 | 170 | 87 | 4 | 32 | 194 | 43 | 195 | 4.21e-27 | 107 |
| MsG0680034383.01.T01 | MTR_6g065470 | 36.842 | 171 | 76 | 3 | 7 | 159 | 6 | 162 | 1.52e-26 | 101 |
| MsG0680034383.01.T01 | MTR_4g086510 | 35.542 | 166 | 89 | 3 | 49 | 205 | 35 | 191 | 1.55e-25 | 102 |
| MsG0680034383.01.T01 | MTR_7g056207 | 36.145 | 166 | 88 | 3 | 49 | 205 | 67 | 223 | 2.41e-25 | 102 |
| MsG0680034383.01.T01 | MTR_4g086540 | 33.862 | 189 | 107 | 5 | 38 | 217 | 33 | 212 | 5.70e-25 | 100 |
| MsG0680034383.01.T01 | MTR_1g012430 | 36.527 | 167 | 90 | 4 | 31 | 195 | 15 | 167 | 4.41e-24 | 98.6 |
| MsG0680034383.01.T01 | MTR_8g035500 | 31.148 | 183 | 108 | 5 | 32 | 205 | 17 | 190 | 8.52e-24 | 97.8 |
| MsG0680034383.01.T01 | MTR_7g406880 | 36.879 | 141 | 78 | 3 | 55 | 189 | 54 | 189 | 1.29e-23 | 97.8 |
| MsG0680034383.01.T01 | MTR_8g035520 | 34.146 | 164 | 94 | 3 | 49 | 205 | 40 | 196 | 1.31e-23 | 97.4 |
| MsG0680034383.01.T01 | MTR_4g086480 | 31.122 | 196 | 110 | 3 | 49 | 227 | 39 | 226 | 1.67e-23 | 97.1 |
| MsG0680034383.01.T01 | MTR_3g101420 | 33.117 | 154 | 83 | 3 | 49 | 192 | 34 | 177 | 2.81e-22 | 93.6 |
| MsG0680034383.01.T01 | MTR_7g107040 | 31.553 | 206 | 110 | 5 | 10 | 205 | 4 | 188 | 1.23e-21 | 92.0 |
| MsG0680034383.01.T01 | MTR_4g050140 | 33.929 | 168 | 92 | 3 | 31 | 192 | 17 | 171 | 1.31e-21 | 92.0 |
| MsG0680034383.01.T01 | MTR_4g086470 | 33.129 | 163 | 95 | 2 | 49 | 205 | 38 | 192 | 3.08e-21 | 90.9 |
| MsG0680034383.01.T01 | MTR_1g111280 | 30.519 | 154 | 87 | 3 | 48 | 192 | 52 | 194 | 2.67e-20 | 89.0 |
| MsG0680034383.01.T01 | MTR_5g005920 | 30.409 | 171 | 103 | 4 | 31 | 193 | 25 | 187 | 4.28e-20 | 88.2 |
| MsG0680034383.01.T01 | MTR_3g012440 | 38.462 | 117 | 66 | 1 | 95 | 205 | 88 | 204 | 5.20e-18 | 82.4 |
| MsG0680034383.01.T01 | MTR_5g005850 | 30.723 | 166 | 92 | 5 | 53 | 205 | 45 | 200 | 7.15e-18 | 81.6 |
| MsG0680034383.01.T01 | MTR_5g005900 | 27.807 | 187 | 112 | 7 | 31 | 205 | 21 | 196 | 9.34e-18 | 81.3 |
| MsG0680034383.01.T01 | MTR_8g040860 | 27.174 | 184 | 102 | 5 | 31 | 201 | 28 | 192 | 2.32e-17 | 80.5 |
| MsG0680034383.01.T01 | MTR_3g450680 | 28.485 | 165 | 97 | 4 | 53 | 205 | 48 | 203 | 2.45e-17 | 80.5 |
| MsG0680034383.01.T01 | MTR_1g105020 | 29.240 | 171 | 98 | 4 | 32 | 192 | 21 | 178 | 3.39e-17 | 79.7 |
| MsG0680034383.01.T01 | MTR_6g022710 | 31.288 | 163 | 69 | 3 | 95 | 221 | 160 | 315 | 1.62e-16 | 78.6 |
| MsG0680034383.01.T01 | MTR_1g104930 | 29.614 | 233 | 124 | 7 | 23 | 243 | 16 | 220 | 6.11e-15 | 73.6 |
| MsG0680034383.01.T01 | MTR_7g051020 | 29.500 | 200 | 114 | 5 | 31 | 218 | 24 | 208 | 1.58e-13 | 69.3 |
| MsG0680034383.01.T01 | MTR_1g104933 | 32.051 | 156 | 86 | 3 | 59 | 205 | 46 | 190 | 1.79e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034383.01.T01 | AT5G23530 | 49.000 | 200 | 87 | 3 | 10 | 205 | 13 | 201 | 1.08e-69 | 218 |
| MsG0680034383.01.T01 | AT5G23530 | 65.854 | 41 | 14 | 0 | 206 | 246 | 288 | 328 | 2.43e-13 | 68.9 |
| MsG0680034383.01.T01 | AT5G27320 | 40.196 | 204 | 116 | 2 | 5 | 205 | 11 | 211 | 1.87e-40 | 142 |
| MsG0680034383.01.T01 | AT3G63010 | 38.389 | 211 | 120 | 4 | 1 | 205 | 7 | 213 | 3.10e-36 | 132 |
| MsG0680034383.01.T01 | AT3G05120 | 37.073 | 205 | 110 | 2 | 7 | 199 | 13 | 210 | 8.55e-35 | 127 |
| MsG0680034383.01.T01 | AT5G62180 | 36.747 | 166 | 100 | 2 | 32 | 196 | 21 | 182 | 2.81e-34 | 126 |
| MsG0680034383.01.T01 | AT1G19190 | 35.165 | 182 | 101 | 4 | 32 | 205 | 18 | 190 | 9.39e-28 | 108 |
| MsG0680034383.01.T01 | AT3G48700 | 36.306 | 157 | 90 | 2 | 49 | 199 | 32 | 184 | 3.38e-27 | 107 |
| MsG0680034383.01.T01 | AT2G45610 | 32.044 | 181 | 110 | 3 | 32 | 205 | 22 | 196 | 5.94e-26 | 103 |
| MsG0680034383.01.T01 | AT2G45600 | 31.646 | 158 | 89 | 2 | 58 | 205 | 38 | 186 | 1.02e-25 | 103 |
| MsG0680034383.01.T01 | AT1G68620 | 39.568 | 139 | 75 | 2 | 56 | 192 | 57 | 188 | 1.56e-25 | 102 |
| MsG0680034383.01.T01 | AT3G48690 | 37.179 | 156 | 85 | 2 | 49 | 198 | 32 | 180 | 3.41e-25 | 101 |
| MsG0680034383.01.T01 | AT5G16080 | 36.943 | 157 | 83 | 4 | 48 | 197 | 54 | 201 | 1.09e-24 | 100 |
| MsG0680034383.01.T01 | AT2G03550 | 34.969 | 163 | 90 | 2 | 49 | 205 | 32 | 184 | 4.01e-24 | 98.6 |
| MsG0680034383.01.T01 | AT1G49640 | 32.941 | 170 | 105 | 4 | 30 | 198 | 17 | 178 | 1.54e-23 | 97.1 |
| MsG0680034383.01.T01 | AT1G47480 | 33.000 | 200 | 102 | 7 | 9 | 205 | 12 | 182 | 2.74e-23 | 96.7 |
| MsG0680034383.01.T01 | AT1G49660 | 33.962 | 159 | 93 | 2 | 53 | 205 | 36 | 188 | 6.08e-23 | 95.5 |
| MsG0680034383.01.T01 | AT1G49650 | 33.987 | 153 | 89 | 2 | 53 | 199 | 92 | 238 | 6.53e-21 | 90.9 |
| MsG0680034383.01.T01 | AT5G14310 | 31.707 | 164 | 65 | 3 | 95 | 218 | 151 | 307 | 5.61e-18 | 83.2 |
| MsG0680034383.01.T01 | AT5G06570 | 39.103 | 156 | 76 | 4 | 60 | 205 | 50 | 196 | 5.72e-17 | 79.3 |
| MsG0680034383.01.T01 | AT5G06570 | 39.103 | 156 | 76 | 4 | 60 | 205 | 50 | 196 | 5.72e-17 | 79.3 |
| MsG0680034383.01.T01 | AT3G27320 | 26.400 | 250 | 99 | 6 | 47 | 218 | 48 | 290 | 6.02e-17 | 80.1 |
| MsG0680034383.01.T01 | AT5G06570 | 39.103 | 156 | 76 | 4 | 60 | 205 | 65 | 211 | 6.17e-17 | 79.3 |
| MsG0680034383.01.T01 | AT5G06570 | 39.103 | 156 | 76 | 4 | 60 | 205 | 65 | 211 | 6.17e-17 | 79.3 |
| MsG0680034383.01.T01 | AT3G27320 | 28.079 | 203 | 91 | 5 | 56 | 218 | 135 | 322 | 2.00e-16 | 78.6 |
Find 78 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATAGCGCCGGTGGAAATT+TGG | 0.269758 | 6:+86677240 | MsG0680034383.01.T01:CDS |
| ACCCGATAACCCGGATATTC+TGG | 0.270729 | 6:-86677300 | None:intergenic |
| CTCTTTACTCCCATTGTTGC+CGG | 0.284162 | 6:+86676926 | MsG0680034383.01.T01:CDS |
| CTTCCAAGGAAGAAGGGGTT+TGG | 0.297350 | 6:-86676723 | None:intergenic |
| AGCGTTAACGGTAACGTCTT+TGG | 0.321214 | 6:-86676882 | None:intergenic |
| GCGGTTAGGGCTTGCAAAGC+GGG | 0.337635 | 6:+86677274 | MsG0680034383.01.T01:CDS |
| GACAGGAAGGGAGATTGTTT+TGG | 0.351128 | 6:-86676975 | None:intergenic |
| GGCACACCATGTTGCGGTTA+GGG | 0.353563 | 6:+86677261 | MsG0680034383.01.T01:CDS |
| CTCCAGAATATCCGGGTTAT+CGG | 0.354754 | 6:+86677298 | MsG0680034383.01.T01:CDS |
| AATAATCAATCATTTATAAC+TGG | 0.374070 | 6:-86677715 | None:intergenic |
| TGGCACACCATGTTGCGGTT+AGG | 0.375335 | 6:+86677260 | MsG0680034383.01.T01:CDS |
| AACCCAAACCCCTTCTTCCT+TGG | 0.375869 | 6:+86676720 | MsG0680034383.01.T01:CDS |
| TGAAAAGCATGAACCATGTT+TGG | 0.377355 | 6:-86677624 | None:intergenic |
| CGACGACAGCAGAGATTTCC+CGG | 0.388643 | 6:-86677078 | None:intergenic |
| TGAGTAGATTCAGGAAAATC+AGG | 0.404021 | 6:-86677654 | None:intergenic |
| AAGCGGGGCTCCAGAATATC+CGG | 0.405006 | 6:+86677290 | MsG0680034383.01.T01:CDS |
| TTCCAAGGAAGAAGGGGTTT+GGG | 0.406480 | 6:-86676722 | None:intergenic |
| TTTGGTGGAAACGCCGTTAA+CGG | 0.432566 | 6:-86676864 | None:intergenic |
| CCTAACGCCACTCCCGTTAA+CGG | 0.446230 | 6:+86676851 | MsG0680034383.01.T01:CDS |
| TTTGACAAGTCAGCATTTCC+CGG | 0.449205 | 6:-86677202 | None:intergenic |
| TTGGTGGAAACGCCGTTAAC+GGG | 0.454826 | 6:-86676863 | None:intergenic |
| CTGTTTGCCGTAGACTCTGC+CGG | 0.459198 | 6:+86677059 | MsG0680034383.01.T01:CDS |
| TAAAACTGTAAGAAGTGAAA+TGG | 0.461001 | 6:-86676756 | None:intergenic |
| TTAACGCTGAAAATAACGTT+TGG | 0.461518 | 6:+86676897 | MsG0680034383.01.T01:CDS |
| TCCAGAATATCCGGGTTATC+GGG | 0.461680 | 6:+86677299 | MsG0680034383.01.T01:CDS |
| AACAGCATCGTAGAGGTTAG+AGG | 0.470736 | 6:-86677041 | None:intergenic |
| GTGACTTCTCCGGCAACAAT+GGG | 0.479210 | 6:-86676935 | None:intergenic |
| CGCGGACCTTCCAAGGAAGA+AGG | 0.480629 | 6:-86676730 | None:intergenic |
| CCGTTAACGGGAGTGGCGTT+AGG | 0.486028 | 6:-86676851 | None:intergenic |
| TTTGGTGGAACCACCATCTC+CGG | 0.486902 | 6:-86676957 | None:intergenic |
| ATAATCAACTGAGTAGATTC+AGG | 0.498659 | 6:-86677663 | None:intergenic |
| AGATTTCCCGGCAGAGTCTA+CGG | 0.500283 | 6:-86677066 | None:intergenic |
| TATGATTGGTTGAAGAAATG+TGG | 0.503360 | 6:+86677576 | MsG0680034383.01.T01:intron |
| GGTGACTTCTCCGGCAACAA+TGG | 0.510089 | 6:-86676936 | None:intergenic |
| GGAAACGCCGTTAACGGGAG+TGG | 0.511016 | 6:-86676858 | None:intergenic |
| ATCAATATGACCCGATAACC+CGG | 0.516613 | 6:-86677309 | None:intergenic |
| TGGTGCAAGTCGGTAGTTGA+CGG | 0.516831 | 6:-86677104 | None:intergenic |
| ATGGTAGCGCGGACCTTCCA+AGG | 0.517263 | 6:-86676737 | None:intergenic |
| TGCGACGGATAGCGATGCTC+TGG | 0.519802 | 6:-86677124 | None:intergenic |
| ATTTCCACCGGCGCTATCTC+CGG | 0.529772 | 6:-86677236 | None:intergenic |
| TTGTCAAAATGTTTCCTAGC+CGG | 0.531131 | 6:+86677217 | MsG0680034383.01.T01:CDS |
| GCCGGAGAAGTCACCGGAGA+TGG | 0.534767 | 6:+86676944 | MsG0680034383.01.T01:CDS |
| AACGGGAGTGGCGTTAGGTG+AGG | 0.535479 | 6:-86676846 | None:intergenic |
| GCGGACCTTCCAAGGAAGAA+GGG | 0.545035 | 6:-86676729 | None:intergenic |
| GTTAAAGAGACGGCGGTTGA+CGG | 0.545760 | 6:-86676807 | None:intergenic |
| CAAACCCCTTCTTCCTTGGA+AGG | 0.548828 | 6:+86676724 | MsG0680034383.01.T01:CDS |
| ATGATTGGTTGAAGAAATGT+GGG | 0.557528 | 6:+86677577 | MsG0680034383.01.T01:intron |
| TGCGGTTAGGGCTTGCAAAG+CGG | 0.562566 | 6:+86677273 | MsG0680034383.01.T01:CDS |
| ACCATCTCCGGTGACTTCTC+CGG | 0.568498 | 6:-86676945 | None:intergenic |
| TCAGTTGATTATGCAAGTCA+AGG | 0.571369 | 6:+86677674 | MsG0680034383.01.T01:CDS |
| CGGTTAGGGCTTGCAAAGCG+GGG | 0.572111 | 6:+86677275 | MsG0680034383.01.T01:CDS |
| GATGCTTCATGTCGCTCTAA+CGG | 0.583341 | 6:+86676782 | MsG0680034383.01.T01:CDS |
| TGTTTGCCGTAGACTCTGCC+GGG | 0.585420 | 6:+86677060 | MsG0680034383.01.T01:CDS |
| GGAAGAAAATAATGACAGGA+AGG | 0.590861 | 6:-86676988 | None:intergenic |
| ATTGTTGCCGGAGAAGTCAC+CGG | 0.591550 | 6:+86676938 | MsG0680034383.01.T01:CDS |
| GTTAACGGTAACGTCTTTGG+TGG | 0.595706 | 6:-86676879 | None:intergenic |
| CCGTGGAAGAAAATAATGAC+AGG | 0.596483 | 6:-86676992 | None:intergenic |
| CACCGGCGCTATCTCCGGCT+AGG | 0.596783 | 6:-86677231 | None:intergenic |
| AGCGGGGCTCCAGAATATCC+GGG | 0.603800 | 6:+86677291 | MsG0680034383.01.T01:CDS |
| GGAGAAGTCACCGGAGATGG+TGG | 0.603928 | 6:+86676947 | MsG0680034383.01.T01:CDS |
| CTAGCCGGAGATAGCGCCGG+TGG | 0.609370 | 6:+86677232 | MsG0680034383.01.T01:CDS |
| AAATTTGGCACACCATGTTG+CGG | 0.611772 | 6:+86677255 | MsG0680034383.01.T01:CDS |
| GAAGAAAATAATGACAGGAA+GGG | 0.614078 | 6:-86676987 | None:intergenic |
| ATGGTGTGCCAAATTTCCAC+CGG | 0.614422 | 6:-86677248 | None:intergenic |
| TCTCCGTCTTCATATTGCGA+CGG | 0.615711 | 6:-86677139 | None:intergenic |
| CGGACCTTCCAAGGAAGAAG+GGG | 0.620041 | 6:-86676728 | None:intergenic |
| TGCAAGCCCTAACCGCAACA+TGG | 0.630176 | 6:-86677267 | None:intergenic |
| GAAAAGTGAAGCCACCGCCG+TGG | 0.638638 | 6:-86677009 | None:intergenic |
| GCATCGTAGAGGTTAGAGGA+AGG | 0.641381 | 6:-86677037 | None:intergenic |
| CAACAATGGGAGTAAAGAGA+CGG | 0.642848 | 6:-86676922 | None:intergenic |
| GATCGAAGAAGTTAAAGAGA+CGG | 0.645752 | 6:-86676817 | None:intergenic |
| AGCGATGCTCTGGTGCAAGT+CGG | 0.647802 | 6:-86677114 | None:intergenic |
| TATCCGTCGCAATATGAAGA+CGG | 0.658300 | 6:+86677136 | MsG0680034383.01.T01:CDS |
| GTTGATTGAATATCCAAACA+TGG | 0.659983 | 6:+86677611 | MsG0680034383.01.T01:CDS |
| TACGGCAAACAGCATCGTAG+AGG | 0.660757 | 6:-86677048 | None:intergenic |
| TTCCTAGCCGGAGATAGCGC+CGG | 0.669385 | 6:+86677229 | MsG0680034383.01.T01:CDS |
| TAAGAAGTGAAATGGTAGCG+CGG | 0.709848 | 6:-86676748 | None:intergenic |
| CGAAGAAGTTAAAGAGACGG+CGG | 0.716329 | 6:-86676814 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAAACTGTAAGAAGTGAAA+TGG | - | Chr6:86676759-86676778 | None:intergenic | 25.0% |
| ATAATCAACTGAGTAGATTC+AGG | - | Chr6:86677666-86677685 | None:intergenic | 30.0% | |
| GAAGAAAATAATGACAGGAA+GGG | - | Chr6:86676990-86677009 | None:intergenic | 30.0% | |
| GTTGATTGAATATCCAAACA+TGG | + | Chr6:86677611-86677630 | MsG0680034383.01.T01:CDS | 30.0% | |
| TTAACGCTGAAAATAACGTT+TGG | + | Chr6:86676897-86676916 | MsG0680034383.01.T01:CDS | 30.0% | |
| ! | ATGATTGGTTGAAGAAATGT+GGG | + | Chr6:86677577-86677596 | MsG0680034383.01.T01:intron | 30.0% |
| ! | TATGATTGGTTGAAGAAATG+TGG | + | Chr6:86677576-86677595 | MsG0680034383.01.T01:intron | 30.0% |
| !! | AACGTTATTTTCAGCGTTAA+CGG | - | Chr6:86676897-86676916 | None:intergenic | 30.0% |
| ATATTGATCCAACCGTTCTT+CGG | + | Chr6:86677324-86677343 | MsG0680034383.01.T01:intron | 35.0% | |
| CTTTGATTGATTGGCAAAAG+AGG | + | Chr6:86677550-86677569 | MsG0680034383.01.T01:intron | 35.0% | |
| GATCGAAGAAGTTAAAGAGA+CGG | - | Chr6:86676820-86676839 | None:intergenic | 35.0% | |
| GGAAGAAAATAATGACAGGA+AGG | - | Chr6:86676991-86677010 | None:intergenic | 35.0% | |
| GGTTTGATCCTTTGATTGAT+TGG | + | Chr6:86677541-86677560 | MsG0680034383.01.T01:intron | 35.0% | |
| TCAGTTGATTATGCAAGTCA+AGG | + | Chr6:86677674-86677693 | MsG0680034383.01.T01:CDS | 35.0% | |
| TGAGTAGATTCAGGAAAATC+AGG | - | Chr6:86677657-86677676 | None:intergenic | 35.0% | |
| TTGTCAAAATGTTTCCTAGC+CGG | + | Chr6:86677217-86677236 | MsG0680034383.01.T01:CDS | 35.0% | |
| ! | CCTGTCATTATTTTCTTCCA+CGG | + | Chr6:86676992-86677011 | MsG0680034383.01.T01:CDS | 35.0% |
| ! | CTCTTTTGCCAATCAATCAA+AGG | - | Chr6:86677552-86677571 | None:intergenic | 35.0% |
| ! | TGAAAAGCATGAACCATGTT+TGG | - | Chr6:86677627-86677646 | None:intergenic | 35.0% |
| !! | GGCAAAAGAGGTATTATGAT+TGG | + | Chr6:86677562-86677581 | MsG0680034383.01.T01:intron | 35.0% |
| !!! | ATACGCTTGTTTTTGTTGGT+GGG | + | Chr6:86677520-86677539 | MsG0680034383.01.T01:intron | 35.0% |
| !!! | GATGTGGAAAGTGTTTTTAC+CGG | + | Chr6:86677419-86677438 | MsG0680034383.01.T01:intron | 35.0% |
| AAATTTGGCACACCATGTTG+CGG | + | Chr6:86677255-86677274 | MsG0680034383.01.T01:CDS | 40.0% | |
| ATCAATATGACCCGATAACC+CGG | - | Chr6:86677312-86677331 | None:intergenic | 40.0% | |
| CAACAATGGGAGTAAAGAGA+CGG | - | Chr6:86676925-86676944 | None:intergenic | 40.0% | |
| CCAACAAAAACAAGCGTATC+CGG | - | Chr6:86677519-86677538 | None:intergenic | 40.0% | |
| CCGTGGAAGAAAATAATGAC+AGG | - | Chr6:86676995-86677014 | None:intergenic | 40.0% | |
| TATCCGTCGCAATATGAAGA+CGG | + | Chr6:86677136-86677155 | MsG0680034383.01.T01:CDS | 40.0% | |
| TTTGACAAGTCAGCATTTCC+CGG | - | Chr6:86677205-86677224 | None:intergenic | 40.0% | |
| TTTGTCGGGTTTGGATTATC+CGG | + | Chr6:86677497-86677516 | MsG0680034383.01.T01:intron | 40.0% | |
| ! | CGAGAACGTAACAGTTTTAC+CGG | + | Chr6:86677183-86677202 | MsG0680034383.01.T01:CDS | 40.0% |
| ! | GAGAACGTAACAGTTTTACC+GGG | + | Chr6:86677184-86677203 | MsG0680034383.01.T01:CDS | 40.0% |
| ! | GTCATTATTTTCTTCCACGG+CGG | + | Chr6:86676995-86677014 | MsG0680034383.01.T01:CDS | 40.0% |
| ! | TAAGAAGTGAAATGGTAGCG+CGG | - | Chr6:86676751-86676770 | None:intergenic | 40.0% |
| !!! | GATACGCTTGTTTTTGTTGG+TGG | + | Chr6:86677519-86677538 | MsG0680034383.01.T01:intron | 40.0% |
| !!! | TGGAAAGTGTTTTTACCGGA+GGG | + | Chr6:86677423-86677442 | MsG0680034383.01.T01:intron | 40.0% |
| AACAGCATCGTAGAGGTTAG+AGG | - | Chr6:86677044-86677063 | None:intergenic | 45.0% | |
| AGCGTTAACGGTAACGTCTT+TGG | - | Chr6:86676885-86676904 | None:intergenic | 45.0% | |
| AGGCGGAGATTCATCTAGTT+GGG | + | Chr6:86677364-86677383 | MsG0680034383.01.T01:intron | 45.0% | |
| ATGGCGCGGTTAATGTATGT+GGG | + | Chr6:86677460-86677479 | MsG0680034383.01.T01:intron | 45.0% | |
| ATGGTGTGCCAAATTTCCAC+CGG | - | Chr6:86677251-86677270 | None:intergenic | 45.0% | |
| CGAAGAAGTTAAAGAGACGG+CGG | - | Chr6:86676817-86676836 | None:intergenic | 45.0% | |
| CTCCAGAATATCCGGGTTAT+CGG | + | Chr6:86677298-86677317 | MsG0680034383.01.T01:CDS | 45.0% | |
| CTCTTTACTCCCATTGTTGC+CGG | + | Chr6:86676926-86676945 | MsG0680034383.01.T01:CDS | 45.0% | |
| GATGCTTCATGTCGCTCTAA+CGG | + | Chr6:86676782-86676801 | MsG0680034383.01.T01:CDS | 45.0% | |
| GTTAACGGTAACGTCTTTGG+TGG | - | Chr6:86676882-86676901 | None:intergenic | 45.0% | |
| TCCAGAATATCCGGGTTATC+GGG | + | Chr6:86677299-86677318 | MsG0680034383.01.T01:CDS | 45.0% | |
| TCTCCGTCTTCATATTGCGA+CGG | - | Chr6:86677142-86677161 | None:intergenic | 45.0% | |
| TTCCAAGGAAGAAGGGGTTT+GGG | - | Chr6:86676725-86676744 | None:intergenic | 45.0% | |
| TTTGGTGGAAACGCCGTTAA+CGG | - | Chr6:86676867-86676886 | None:intergenic | 45.0% | |
| ! | ATTATTTTCTTCCACGGCGG+TGG | + | Chr6:86676998-86677017 | MsG0680034383.01.T01:CDS | 45.0% |
| ! | GACAGGAAGGGAGATTGTTT+TGG | - | Chr6:86676978-86676997 | None:intergenic | 45.0% |
| !! | AGTGTTTTTACCGGAGGGAT+CGG | + | Chr6:86677428-86677447 | MsG0680034383.01.T01:intron | 45.0% |
| !!! | AGGAAGGGAGATTGTTTTGG+TGG | - | Chr6:86676975-86676994 | None:intergenic | 45.0% |
| !!! | CCGGATACGCTTGTTTTTGT+TGG | + | Chr6:86677516-86677535 | MsG0680034383.01.T01:intron | 45.0% |
| !!! | GTGGAAAGTGTTTTTACCGG+AGG | + | Chr6:86677422-86677441 | MsG0680034383.01.T01:intron | 45.0% |
| AACCCAAACCCCTTCTTCCT+TGG | + | Chr6:86676720-86676739 | MsG0680034383.01.T01:CDS | 50.0% | |
| ACCCGATAACCCGGATATTC+TGG | - | Chr6:86677303-86677322 | None:intergenic | 50.0% | |
| AGATTTCCCGGCAGAGTCTA+CGG | - | Chr6:86677069-86677088 | None:intergenic | 50.0% | |
| ATTGTTGCCGGAGAAGTCAC+CGG | + | Chr6:86676938-86676957 | MsG0680034383.01.T01:CDS | 50.0% | |
| CAAACCCCTTCTTCCTTGGA+AGG | + | Chr6:86676724-86676743 | MsG0680034383.01.T01:CDS | 50.0% | |
| CATGGCGCGGTTAATGTATG+TGG | + | Chr6:86677459-86677478 | MsG0680034383.01.T01:intron | 50.0% | |
| CGTTCTTCGGAGGAGAAGAA+CGG | + | Chr6:86677337-86677356 | MsG0680034383.01.T01:intron | 50.0% | |
| CGTTCTTCTCCTCCGAAGAA+CGG | - | Chr6:86677339-86677358 | None:intergenic | 50.0% | |
| CTTCCAAGGAAGAAGGGGTT+TGG | - | Chr6:86676726-86676745 | None:intergenic | 50.0% | |
| GAGGCGGAGATTCATCTAGT+TGG | + | Chr6:86677363-86677382 | MsG0680034383.01.T01:intron | 50.0% | |
| GCATCGTAGAGGTTAGAGGA+AGG | - | Chr6:86677040-86677059 | None:intergenic | 50.0% | |
| GTCCTTGCCACTGACACAAA+CGG | - | Chr6:86677393-86677412 | None:intergenic | 50.0% | |
| GTGACTTCTCCGGCAACAAT+GGG | - | Chr6:86676938-86676957 | None:intergenic | 50.0% | |
| GTTAAAGAGACGGCGGTTGA+CGG | - | Chr6:86676810-86676829 | None:intergenic | 50.0% | |
| TAATGTATGTGGGCCGAACG+TGG | + | Chr6:86677470-86677489 | MsG0680034383.01.T01:intron | 50.0% | |
| TACGGCAAACAGCATCGTAG+AGG | - | Chr6:86677051-86677070 | None:intergenic | 50.0% | |
| TGGCAAGGACTGATTGGATG+TGG | + | Chr6:86677403-86677422 | MsG0680034383.01.T01:intron | 50.0% | |
| TGGTGCAAGTCGGTAGTTGA+CGG | - | Chr6:86677107-86677126 | None:intergenic | 50.0% | |
| TGTCAGTGGCAAGGACTGAT+TGG | + | Chr6:86677397-86677416 | MsG0680034383.01.T01:intron | 50.0% | |
| TTGATCCAACCGTTCTTCGG+AGG | + | Chr6:86677327-86677346 | MsG0680034383.01.T01:intron | 50.0% | |
| TTGGTGGAAACGCCGTTAAC+GGG | - | Chr6:86676866-86676885 | None:intergenic | 50.0% | |
| TTTGGTGGAACCACCATCTC+CGG | - | Chr6:86676960-86676979 | None:intergenic | 50.0% | |
| !! | AGATAGCGCCGGTGGAAATT+TGG | + | Chr6:86677240-86677259 | MsG0680034383.01.T01:CDS | 50.0% |
| AAGCGGGGCTCCAGAATATC+CGG | + | Chr6:86677290-86677309 | MsG0680034383.01.T01:CDS | 55.0% | |
| ACCATCTCCGGTGACTTCTC+CGG | - | Chr6:86676948-86676967 | None:intergenic | 55.0% | |
| AGCGATGCTCTGGTGCAAGT+CGG | - | Chr6:86677117-86677136 | None:intergenic | 55.0% | |
| ATTTCCACCGGCGCTATCTC+CGG | - | Chr6:86677239-86677258 | None:intergenic | 55.0% | |
| CATTAACCGCGCCATGATCC+CGG | - | Chr6:86677455-86677474 | None:intergenic | 55.0% | |
| CCCGACAAATCCTCCACGTT+CGG | - | Chr6:86677486-86677505 | None:intergenic | 55.0% | |
| CCGAACGTGGAGGATTTGTC+GGG | + | Chr6:86677483-86677502 | MsG0680034383.01.T01:intron | 55.0% | |
| CCTAACGCCACTCCCGTTAA+CGG | + | Chr6:86676851-86676870 | MsG0680034383.01.T01:CDS | 55.0% | |
| CGACGACAGCAGAGATTTCC+CGG | - | Chr6:86677081-86677100 | None:intergenic | 55.0% | |
| CGGACCTTCCAAGGAAGAAG+GGG | - | Chr6:86676731-86676750 | None:intergenic | 55.0% | |
| CGTGGAGGATTTGTCGGGTT+TGG | + | Chr6:86677488-86677507 | MsG0680034383.01.T01:intron | 55.0% | |
| CTGTTTGCCGTAGACTCTGC+CGG | + | Chr6:86677059-86677078 | MsG0680034383.01.T01:CDS | 55.0% | |
| CTTCGGAGGAGAAGAACGGA+CGG | + | Chr6:86677341-86677360 | MsG0680034383.01.T01:intron | 55.0% | |
| CTTCTCCTCCGAAGAACGGT+TGG | - | Chr6:86677335-86677354 | None:intergenic | 55.0% | |
| GCCGAACGTGGAGGATTTGT+CGG | + | Chr6:86677482-86677501 | MsG0680034383.01.T01:intron | 55.0% | |
| GCGGACCTTCCAAGGAAGAA+GGG | - | Chr6:86676732-86676751 | None:intergenic | 55.0% | |
| GGCACACCATGTTGCGGTTA+GGG | + | Chr6:86677261-86677280 | MsG0680034383.01.T01:CDS | 55.0% | |
| GGTGACTTCTCCGGCAACAA+TGG | - | Chr6:86676939-86676958 | None:intergenic | 55.0% | |
| TGCAAGCCCTAACCGCAACA+TGG | - | Chr6:86677270-86677289 | None:intergenic | 55.0% | |
| TGCGGTTAGGGCTTGCAAAG+CGG | + | Chr6:86677273-86677292 | MsG0680034383.01.T01:CDS | 55.0% | |
| TGGCACACCATGTTGCGGTT+AGG | + | Chr6:86677260-86677279 | MsG0680034383.01.T01:CDS | 55.0% | |
| TGTTTGCCGTAGACTCTGCC+GGG | + | Chr6:86677060-86677079 | MsG0680034383.01.T01:CDS | 55.0% | |
| ! | TGCCGTTTGTGTCAGTGGCA+AGG | + | Chr6:86677388-86677407 | MsG0680034383.01.T01:intron | 55.0% |
| ! | TGGGATGCCGTTTGTGTCAG+TGG | + | Chr6:86677383-86677402 | MsG0680034383.01.T01:intron | 55.0% |
| ! | TTTTACCGGAGGGATCGGAC+CGG | + | Chr6:86677433-86677452 | MsG0680034383.01.T01:intron | 55.0% |
| AACGGGAGTGGCGTTAGGTG+AGG | - | Chr6:86676849-86676868 | None:intergenic | 60.0% | |
| AGCGGGGCTCCAGAATATCC+GGG | + | Chr6:86677291-86677310 | MsG0680034383.01.T01:CDS | 60.0% | |
| AGGAGAAGAACGGACGGAGG+CGG | + | Chr6:86677347-86677366 | MsG0680034383.01.T01:intron | 60.0% | |
| CCGTTAACGGGAGTGGCGTT+AGG | - | Chr6:86676854-86676873 | None:intergenic | 60.0% | |
| CGCGGACCTTCCAAGGAAGA+AGG | - | Chr6:86676733-86676752 | None:intergenic | 60.0% | |
| CGGTTAGGGCTTGCAAAGCG+GGG | + | Chr6:86677275-86677294 | MsG0680034383.01.T01:CDS | 60.0% | |
| GAAAAGTGAAGCCACCGCCG+TGG | - | Chr6:86677012-86677031 | None:intergenic | 60.0% | |
| GCGGTTAGGGCTTGCAAAGC+GGG | + | Chr6:86677274-86677293 | MsG0680034383.01.T01:CDS | 60.0% | |
| GGAAACGCCGTTAACGGGAG+TGG | - | Chr6:86676861-86676880 | None:intergenic | 60.0% | |
| GGAGAAGTCACCGGAGATGG+TGG | + | Chr6:86676947-86676966 | MsG0680034383.01.T01:CDS | 60.0% | |
| TGCGACGGATAGCGATGCTC+TGG | - | Chr6:86677127-86677146 | None:intergenic | 60.0% | |
| TGTATGTGGGCCGAACGTGG+AGG | + | Chr6:86677473-86677492 | MsG0680034383.01.T01:intron | 60.0% | |
| TTCCTAGCCGGAGATAGCGC+CGG | + | Chr6:86677229-86677248 | MsG0680034383.01.T01:CDS | 60.0% | |
| TTTACCGGAGGGATCGGACC+GGG | + | Chr6:86677434-86677453 | MsG0680034383.01.T01:intron | 60.0% | |
| ! | ATGGTAGCGCGGACCTTCCA+AGG | - | Chr6:86676740-86676759 | None:intergenic | 60.0% |
| ATCGGACCGGGATCATGGCG+CGG | + | Chr6:86677446-86677465 | MsG0680034383.01.T01:intron | 65.0% | |
| CGGAGGAGAAGAACGGACGG+AGG | + | Chr6:86677344-86677363 | MsG0680034383.01.T01:intron | 65.0% | |
| GAGGGATCGGACCGGGATCA+TGG | + | Chr6:86677441-86677460 | MsG0680034383.01.T01:intron | 65.0% | |
| GCCGGAGAAGTCACCGGAGA+TGG | + | Chr6:86676944-86676963 | MsG0680034383.01.T01:CDS | 65.0% | |
| TGATCCCGGTCCGATCCCTC+CGG | - | Chr6:86677441-86677460 | None:intergenic | 65.0% | |
| CACCGGCGCTATCTCCGGCT+AGG | - | Chr6:86677234-86677253 | None:intergenic | 70.0% | |
| ! | CTAGCCGGAGATAGCGCCGG+TGG | + | Chr6:86677232-86677251 | MsG0680034383.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 86676707 | 86677728 | 86676707 | ID=MsG0680034383.01;Name=MsG0680034383.01 |
| Chr6 | mRNA | 86676707 | 86677728 | 86676707 | ID=MsG0680034383.01.T01;Parent=MsG0680034383.01;Name=MsG0680034383.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|251 |
| Chr6 | exon | 86676707 | 86677320 | 86676707 | ID=MsG0680034383.01.T01:exon:18245;Parent=MsG0680034383.01.T01 |
| Chr6 | exon | 86677587 | 86677728 | 86677587 | ID=MsG0680034383.01.T01:exon:18246;Parent=MsG0680034383.01.T01 |
| Chr6 | CDS | 86676707 | 86677320 | 86676707 | ID=MsG0680034383.01.T01:cds;Parent=MsG0680034383.01.T01 |
| Chr6 | CDS | 86677587 | 86677728 | 86677587 | ID=MsG0680034383.01.T01:cds;Parent=MsG0680034383.01.T01 |
| Chr6 | mRNA | 86676783 | 86677172 | 86676783 | ID=MsG0680034383.01.T02;Parent=MsG0680034383.01;Name=MsG0680034383.01.T02;_AED=0.50;_eAED=1.00;_QI=0|0|0|1|0|0|2|0|118 |
| Chr6 | exon | 86676783 | 86676948 | 86676783 | ID=MsG0680034383.01.T02:exon:18247;Parent=MsG0680034383.01.T02 |
| Chr6 | exon | 86676982 | 86677172 | 86676982 | ID=MsG0680034383.01.T02:exon:18248;Parent=MsG0680034383.01.T02 |
| Chr6 | CDS | 86676783 | 86676948 | 86676783 | ID=MsG0680034383.01.T02:cds;Parent=MsG0680034383.01.T02 |
| Chr6 | CDS | 86676982 | 86677172 | 86676982 | ID=MsG0680034383.01.T02:cds;Parent=MsG0680034383.01.T02 |
| Gene Sequence |
| Protein sequence |
