AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018338.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018338.01.T01 MTR_4g010140 78.723 94 6 1 1 94 50 129 8.25e-40 131
MsG0480018338.01.T01 MTR_4g010140 78.723 94 6 1 1 94 50 129 2.36e-39 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018338.01.T01 AT3G15840 57.647 85 22 1 10 94 28 98 2.67e-27 100
MsG0480018338.01.T01 AT3G15840 74.138 58 15 0 37 94 7 64 4.37e-27 99.0
MsG0480018338.01.T01 AT3G15840 57.647 85 22 1 10 94 48 118 4.50e-27 100
MsG0480018338.01.T01 AT3G15840 57.647 85 22 1 10 94 48 118 4.75e-27 100
MsG0480018338.01.T01 AT3G15840 54.118 85 22 2 10 94 48 115 1.02e-22 88.6

Find 36 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTAATATTGGATTGAAATA+AGG 0.213857 4:+3509411 None:intergenic
CTTGCTCCTAGTGAAGAATT+TGG 0.251751 4:-3507793 MsG0480018338.01.T01:CDS
CTTATTTCAATCCAATATTA+AGG 0.264128 4:-3509410 MsG0480018338.01.T01:five_prime_UTR
TATATCCTATGAATGTTTAT+AGG 0.297576 4:-3508022 MsG0480018338.01.T01:CDS
ATAAACATTCATAGGATATA+TGG 0.352487 4:+3508025 None:intergenic
TAAATAGAAGAGAGAAAGAT+TGG 0.387202 4:+3509442 None:intergenic
ACCAAGATGTTACCCTTTGA+AGG 0.390463 4:-3508586 MsG0480018338.01.T01:five_prime_UTR
TTCATACTCTTGAATTTCTT+CGG 0.394407 4:+3508503 None:intergenic
TTGGGAAGGCTGCTGTCTAC+TGG 0.402887 4:-3507968 MsG0480018338.01.T01:CDS
ATAGGTACAAGCTTCCTTCA+TGG 0.408186 4:-3508004 MsG0480018338.01.T01:CDS
TCCTTCAAAGGGTAACATCT+TGG 0.415937 4:+3508585 None:intergenic
TTCATATGTCTTTCCTTCAA+AGG 0.436295 4:+3508573 None:intergenic
TCATGGGCTATGTTTGAACT+TGG 0.438144 4:-3507987 MsG0480018338.01.T01:CDS
TCATATGTCTTTCCTTCAAA+GGG 0.449365 4:+3508574 None:intergenic
TTGTACCTATAAACATTCAT+AGG 0.453901 4:+3508017 None:intergenic
TAGGTACAAGCTTCCTTCAT+GGG 0.479108 4:-3508003 MsG0480018338.01.T01:CDS
GGAGCAAGTTGAGCTGCAGC+TGG 0.485205 4:+3507808 None:intergenic
CATAGCATAACAAACACGTT+TGG 0.489492 4:-3509293 MsG0480018338.01.T01:intron
AATATCATACTTACTGAAGT+AGG 0.495204 4:+3507924 None:intergenic
GCGATTCCAAATTCTTCACT+AGG 0.503363 4:+3507787 None:intergenic
GGCTATGTTTGAACTTGGGA+AGG 0.522989 4:-3507982 MsG0480018338.01.T01:CDS
TACTCTTGAATTTCTTCGGT+AGG 0.541328 4:+3508507 None:intergenic
CATGGGCTATGTTTGAACTT+GGG 0.542521 4:-3507986 MsG0480018338.01.T01:CDS
AGTAGGAGCAACACCATTGG+TGG 0.543782 4:+3507941 None:intergenic
TTGTGACAGAGAAGCAAGGT+TGG 0.548794 4:+3509327 None:intergenic
TGAAGTCAGTGCTGCTGCTG+CGG 0.560072 4:-3508541 MsG0480018338.01.T01:CDS
TGTGACAGAGAAGCAAGGTT+GGG 0.560846 4:+3509328 None:intergenic
ACATTCATAGGATATATGGC+AGG 0.565650 4:+3508029 None:intergenic
AGTTCAAACATAGCCCATGA+AGG 0.591842 4:+3507990 None:intergenic
GACAGAGAAGCAAGGTTGGG+TGG 0.594710 4:+3509331 None:intergenic
CATTCATAGGATATATGGCA+GGG 0.600651 4:+3508030 None:intergenic
TGAAGTAGGAGCAACACCAT+TGG 0.613214 4:+3507938 None:intergenic
ATAGAAGAGAGAAAGATTGG+TGG 0.623074 4:+3509445 None:intergenic
GTCTACTGGAAAACCACCAA+TGG 0.649539 4:-3507954 MsG0480018338.01.T01:CDS
TGTTATGCTATGACTACGAG+AGG 0.652151 4:+3509304 None:intergenic
GAGGTTGTGACAGAGAAGCA+AGG 0.671049 4:+3509323 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTATTTATTTTTTAATAG+TGG - Chr4:3508035-3508054 MsG0480018338.01.T01:CDS 10.0%
!! TAGAAATACAGAAAATATAA+AGG - Chr4:3508992-3509011 MsG0480018338.01.T01:intron 15.0%
!!! ATTTATTTTTTAATAGTGGA+CGG - Chr4:3508039-3508058 MsG0480018338.01.T01:CDS 15.0%
!!! GTTATTCTTTTAAACTTATT+GGG - Chr4:3508253-3508272 MsG0480018338.01.T01:intron 15.0%
!! ACTCAAAAATGAAAAGAAAA+AGG + Chr4:3507970-3507989 None:intergenic 20.0%
!! ATAAACATTCATAGGATATA+TGG + Chr4:3509206-3509225 None:intergenic 20.0%
!! CTCAAAAATGAAAAGAAAAA+GGG + Chr4:3507969-3507988 None:intergenic 20.0%
!! CTTAATATTGGATTGAAATA+AGG + Chr4:3507820-3507839 None:intergenic 20.0%
!! CTTATTTCAATCCAATATTA+AGG - Chr4:3507818-3507837 MsG0480018338.01.T01:CDS 20.0%
!! TATATCCTATGAATGTTTAT+AGG - Chr4:3509206-3509225 MsG0480018338.01.T01:intron 20.0%
!! TCATATAATAAAGTTCTTAG+TGG + Chr4:3508190-3508209 None:intergenic 20.0%
!!! AAAGAATAACCATTTTCTTT+TGG + Chr4:3508243-3508262 None:intergenic 20.0%
!!! ATTTTTTTATAAACTTTGGC+AGG - Chr4:3509371-3509390 MsG0480018338.01.T01:intron 20.0%
!!! GGTTATTCTTTTAAACTTAT+TGG - Chr4:3508252-3508271 MsG0480018338.01.T01:intron 20.0%
!!! TATAGTATTTTGATGAAGTT+AGG - Chr4:3509131-3509150 MsG0480018338.01.T01:intron 20.0%
!!! TATTCAAACATTTTTTAACC+AGG + Chr4:3508492-3508511 None:intergenic 20.0%
!!! TCGTAATTCTTTTGATTAAA+GGG - Chr4:3508217-3508236 MsG0480018338.01.T01:intron 20.0%
!!! TTCGTAATTCTTTTGATTAA+AGG - Chr4:3508216-3508235 MsG0480018338.01.T01:intron 20.0%
!!! TTTGTTCATGTCTTTATTTA+TGG + Chr4:3509058-3509077 None:intergenic 20.0%
!!! TTTTTTTATAAACTTTGGCA+GGG - Chr4:3509372-3509391 MsG0480018338.01.T01:intron 20.0%
! AAAAAAAAAACGTGGTTGAA+AGG - Chr4:3508404-3508423 MsG0480018338.01.T01:intron 25.0%
! AATATCATACTTACTGAAGT+AGG + Chr4:3509307-3509326 None:intergenic 25.0%
! ATCAATTCAATGATAGACTT+GGG + Chr4:3508120-3508139 None:intergenic 25.0%
! CAAATAATTAGACAGTAAAC+TGG - Chr4:3508936-3508955 MsG0480018338.01.T01:intron 25.0%
! CAATGGAGAAAAGTTAATAT+AGG - Chr4:3508776-3508795 MsG0480018338.01.T01:intron 25.0%
! GAAAGGATAAATCATACTTA+AGG - Chr4:3508421-3508440 MsG0480018338.01.T01:intron 25.0%
! TAAATAGAAGAGAGAAAGAT+TGG + Chr4:3507789-3507808 None:intergenic 25.0%
! TATCAATTCAATGATAGACT+TGG + Chr4:3508121-3508140 None:intergenic 25.0%
! TGTAGATGAAAAAAAAAACG+TGG - Chr4:3508396-3508415 MsG0480018338.01.T01:intron 25.0%
! TTACTGTCTAATTATTTGAG+TGG + Chr4:3508934-3508953 None:intergenic 25.0%
! TTCATACTCTTGAATTTCTT+CGG + Chr4:3508728-3508747 None:intergenic 25.0%
! TTGTACCTATAAACATTCAT+AGG + Chr4:3509214-3509233 None:intergenic 25.0%
!! AATATCAGAGTCTAGTTTAA+TGG + Chr4:3508350-3508369 None:intergenic 25.0%
!! ATTTGATTATTTGTTGACTG+AGG - Chr4:3508074-3508093 MsG0480018338.01.T01:intron 25.0%
!!! GTTTTAAAAACTGACTCAAA+CGG + Chr4:3508967-3508986 None:intergenic 25.0%
!!! TGATATTTTAAGCAAAGTCT+CGG - Chr4:3508363-3508382 MsG0480018338.01.T01:intron 25.0%
!!! TTTTTTTTCATCTACAAAGC+TGG + Chr4:3508392-3508411 None:intergenic 25.0%
AATCCAACATTGGTATTAAG+TGG + Chr4:3508754-3508773 None:intergenic 30.0%
ACTGAAAACTAATCCTCATT+TGG + Chr4:3508461-3508480 None:intergenic 30.0%
AGGATAAATCATACTTAAGG+TGG - Chr4:3508424-3508443 MsG0480018338.01.T01:intron 30.0%
ATCCAACATTGGTATTAAGT+GGG + Chr4:3508753-3508772 None:intergenic 30.0%
ATTAAAGGGCCAAAAGAAAA+TGG - Chr4:3508231-3508250 MsG0480018338.01.T01:intron 30.0%
TACCAATGTTGGATTTACAA+TGG - Chr4:3508759-3508778 MsG0480018338.01.T01:intron 30.0%
TCATATGTCTTTCCTTCAAA+GGG + Chr4:3508657-3508676 None:intergenic 30.0%
TTCATATGTCTTTCCTTCAA+AGG + Chr4:3508658-3508677 None:intergenic 30.0%
! AGTTTTCAGTTAAGTAAACC+TGG - Chr4:3508471-3508490 MsG0480018338.01.T01:intron 30.0%
! GTTCATGTCTTTATTTATGG+TGG + Chr4:3509055-3509074 None:intergenic 30.0%
!! GAATTTGGAATCGCTTTTAA+TGG - Chr4:3509450-3509469 MsG0480018338.01.T01:five_prime_UTR 30.0%
!! TTTTGGTGACTCCTTAATAT+TGG + Chr4:3507832-3507851 None:intergenic 30.0%
!!! CAGTGTTTTGAAAACTGAAA+TGG - Chr4:3508832-3508851 MsG0480018338.01.T01:intron 30.0%
ACATTCATAGGATATATGGC+AGG + Chr4:3509202-3509221 None:intergenic 35.0%
ATAGAAGAGAGAAAGATTGG+TGG + Chr4:3507786-3507805 None:intergenic 35.0%
CATAGCATAACAAACACGTT+TGG - Chr4:3507935-3507954 MsG0480018338.01.T01:intron 35.0%
CATTCATAGGATATATGGCA+GGG + Chr4:3509201-3509220 None:intergenic 35.0%
CTCCATTGTAAATCCAACAT+TGG + Chr4:3508764-3508783 None:intergenic 35.0%
TACTCTTGAATTTCTTCGGT+AGG + Chr4:3508724-3508743 None:intergenic 35.0%
TTCCCACTTAATACCAATGT+TGG - Chr4:3508748-3508767 MsG0480018338.01.T01:intron 35.0%
TTTAAATCACTCGAGCAAAG+AGG - Chr4:3508895-3508914 MsG0480018338.01.T01:intron 35.0%
! CACCAAAAAAGTGTGATACA+TGG - Chr4:3507844-3507863 MsG0480018338.01.T01:intron 35.0%
!!! AGCCATGTATCACACTTTTT+TGG + Chr4:3507849-3507868 None:intergenic 35.0%
ACCAAGATGTTACCCTTTGA+AGG - Chr4:3508642-3508661 MsG0480018338.01.T01:intron 40.0%
AGTTCAAACATAGCCCATGA+AGG + Chr4:3509241-3509260 None:intergenic 40.0%
ATAGGTACAAGCTTCCTTCA+TGG - Chr4:3509224-3509243 MsG0480018338.01.T01:intron 40.0%
CACATGACCAATTGCAAAGA+TGG + Chr4:3507996-3508015 None:intergenic 40.0%
CACTTCATAGTTTCTGATGC+AGG - Chr4:3508595-3508614 MsG0480018338.01.T01:five_prime_UTR 40.0%
CATCTTTGCAATTGGTCATG+TGG - Chr4:3507994-3508013 MsG0480018338.01.T01:CDS 40.0%
CTTGCTCCTAGTGAAGAATT+TGG - Chr4:3509435-3509454 MsG0480018338.01.T01:five_prime_UTR 40.0%
GCGATTCCAAATTCTTCACT+AGG + Chr4:3509444-3509463 None:intergenic 40.0%
GTCATTGAACCATGACTAGA+AGG + Chr4:3508875-3508894 None:intergenic 40.0%
TAGGTACAAGCTTCCTTCAT+GGG - Chr4:3509225-3509244 MsG0480018338.01.T01:intron 40.0%
TCCTTCAAAGGGTAACATCT+TGG + Chr4:3508646-3508665 None:intergenic 40.0%
TGTTATGCTATGACTACGAG+AGG + Chr4:3507927-3507946 None:intergenic 40.0%
! CATGGGCTATGTTTGAACTT+GGG - Chr4:3509242-3509261 MsG0480018338.01.T01:intron 40.0%
! GCAATTGGTCATGTGGATTA+TGG - Chr4:3508001-3508020 MsG0480018338.01.T01:CDS 40.0%
! TCATGGGCTATGTTTGAACT+TGG - Chr4:3509241-3509260 MsG0480018338.01.T01:intron 40.0%
CATACTTAAGGTGGTCAACG+AGG - Chr4:3508433-3508452 MsG0480018338.01.T01:intron 45.0%
GAACCATGACTAGAAGGTCT+TGG + Chr4:3508869-3508888 None:intergenic 45.0%
GAGTGCACCATCTTTGCAAT+TGG - Chr4:3507986-3508005 MsG0480018338.01.T01:CDS 45.0%
GGCTATGTTTGAACTTGGGA+AGG - Chr4:3509246-3509265 MsG0480018338.01.T01:intron 45.0%
GTCTACTGGAAAACCACCAA+TGG - Chr4:3509274-3509293 MsG0480018338.01.T01:intron 45.0%
TGAAGTAGGAGCAACACCAT+TGG + Chr4:3509293-3509312 None:intergenic 45.0%
TGTGACAGAGAAGCAAGGTT+GGG + Chr4:3507903-3507922 None:intergenic 45.0%
TTGTGACAGAGAAGCAAGGT+TGG + Chr4:3507904-3507923 None:intergenic 45.0%
!!! TAATATTTTTTTATAAACTT+TGG - Chr4:3509367-3509386 MsG0480018338.01.T01:intron 5.0%
AGTAGGAGCAACACCATTGG+TGG + Chr4:3509290-3509309 None:intergenic 50.0%
GAGGTTGTGACAGAGAAGCA+AGG + Chr4:3507908-3507927 None:intergenic 50.0%
GGTCAACGAGGTTCCAAATG+AGG - Chr4:3508445-3508464 MsG0480018338.01.T01:intron 50.0%
TCGCCAAGACCTTCTAGTCA+TGG - Chr4:3508863-3508882 MsG0480018338.01.T01:intron 50.0%
GACAGAGAAGCAAGGTTGGG+TGG + Chr4:3507900-3507919 None:intergenic 55.0%
TTGGGAAGGCTGCTGTCTAC+TGG - Chr4:3509260-3509279 MsG0480018338.01.T01:intron 55.0%
! TGAAGTCAGTGCTGCTGCTG+CGG - Chr4:3508687-3508706 MsG0480018338.01.T01:intron 55.0%
! GGAGCAAGTTGAGCTGCAGC+TGG + Chr4:3509423-3509442 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 3507756 3509494 3507756 ID=MsG0480018338.01;Name=MsG0480018338.01
Chr4 mRNA 3507756 3509494 3507756 ID=MsG0480018338.01.T01;Parent=MsG0480018338.01;Name=MsG0480018338.01.T01;_AED=0.46;_eAED=0.51;_QI=234|0.75|0.6|1|0.5|0.4|5|0|98
Chr4 exon 3507756 3507857 3507756 ID=MsG0480018338.01.T01:exon:1013;Parent=MsG0480018338.01.T01
Chr4 exon 3507938 3508064 3507938 ID=MsG0480018338.01.T01:exon:1012;Parent=MsG0480018338.01.T01
Chr4 exon 3508510 3508633 3508510 ID=MsG0480018338.01.T01:exon:1011;Parent=MsG0480018338.01.T01
Chr4 exon 3509294 3509377 3509294 ID=MsG0480018338.01.T01:exon:1010;Parent=MsG0480018338.01.T01
Chr4 exon 3509401 3509494 3509401 ID=MsG0480018338.01.T01:exon:1009;Parent=MsG0480018338.01.T01
Chr4 five_prime_UTR 3509401 3509494 3509401 ID=MsG0480018338.01.T01:five_prime_utr;Parent=MsG0480018338.01.T01
Chr4 five_prime_UTR 3509294 3509377 3509294 ID=MsG0480018338.01.T01:five_prime_utr;Parent=MsG0480018338.01.T01
Chr4 five_prime_UTR 3508578 3508633 3508578 ID=MsG0480018338.01.T01:five_prime_utr;Parent=MsG0480018338.01.T01
Chr4 CDS 3508510 3508577 3508510 ID=MsG0480018338.01.T01:cds;Parent=MsG0480018338.01.T01
Chr4 CDS 3507938 3508064 3507938 ID=MsG0480018338.01.T01:cds;Parent=MsG0480018338.01.T01
Chr4 CDS 3507756 3507857 3507756 ID=MsG0480018338.01.T01:cds;Parent=MsG0480018338.01.T01
Gene Sequence

>MsG0480018338.01.T01

ATGAAAGTTAAGCATGAAGTCAGTGCTGCTGCTGCGGTTGCAACAAGTCCTACCGAAGAAATTCAAGAATGCAATAGTAACCCTGCCATATATCCTATGAATGTTTATAGGTACAAGCTTCCTTCATGGGCTATGTTTGAACTTGGGAAGGCTGCTGTCTACTGGAAAACCACCAATGGTGTTGCTCCTACTTCAGGTGAAAAGCTTAAACTGTTCTATAATCCAGCTGCAGCTCAACTTGCTCCTAGTGAAGAATTTGGAATCGCTTTTAATGGTAATTTTGTTGATGTTGCTTAG

Protein sequence

>MsG0480018338.01.T01

MKVKHEVSAAAAVATSPTEEIQECNSNPAIYPMNVYRYKLPSWAMFELGKAAVYWKTTNGVAPTSGEKLKLFYNPAAAQLAPSEEFGIAFNGNFVDVA*