AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034732.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034732.01.T01 MTR_6g073110 95.364 302 14 0 1 302 17 318 0.0 598
MsG0680034732.01.T01 MTR_4g072350 62.583 302 113 0 1 302 17 318 1.67e-148 419
MsG0680034732.01.T01 MTR_4g072360 61.589 302 116 0 1 302 17 318 2.77e-146 414
MsG0680034732.01.T01 MTR_4g072320 61.921 302 109 2 1 302 17 312 4.28e-142 403
MsG0680034732.01.T01 MTR_0374s0050 52.980 302 134 1 1 302 20 313 4.03e-108 317
MsG0680034732.01.T01 MTR_3g083130 51.568 287 131 3 1 284 21 302 2.57e-98 292
MsG0680034732.01.T01 MTR_5g097900 52.113 284 133 3 1 284 20 300 9.47e-89 267
MsG0680034732.01.T01 MTR_5g097910 52.113 284 133 3 1 284 20 300 1.19e-88 267
MsG0680034732.01.T01 MTR_5g097940 52.113 284 133 3 1 284 20 300 1.19e-88 267
MsG0680034732.01.T01 MTR_4g036845 44.224 303 164 4 1 302 14 312 1.19e-84 257
MsG0680034732.01.T01 MTR_8g070095 39.672 305 177 5 1 302 16 316 7.14e-75 232
MsG0680034732.01.T01 MTR_8g070115 39.051 274 163 3 30 302 42 312 4.84e-69 217
MsG0680034732.01.T01 MTR_7g063580 39.560 273 160 4 31 302 83 351 8.06e-65 207
MsG0680034732.01.T01 MTR_4g021350 39.716 282 164 4 22 302 34 310 4.34e-63 202
MsG0680034732.01.T01 MTR_7g070500 35.948 306 182 7 1 302 23 318 3.34e-60 195
MsG0680034732.01.T01 MTR_4g021450 38.284 303 169 5 1 302 17 302 4.55e-59 191
MsG0680034732.01.T01 MTR_4g021350 40.467 257 148 3 22 278 34 285 1.36e-58 189
MsG0680034732.01.T01 MTR_4g021410 38.614 303 168 6 1 302 17 302 4.70e-58 189
MsG0680034732.01.T01 MTR_4g021410 39.711 277 152 5 27 302 3 265 1.53e-57 186
MsG0680034732.01.T01 MTR_4g072060 39.310 290 156 7 1 278 11 292 1.21e-56 185
MsG0680034732.01.T01 MTR_7g070500 36.101 277 166 5 1 276 23 289 1.59e-55 182
MsG0680034732.01.T01 MTR_6g072650 53.061 147 47 1 156 302 10 134 8.91e-48 157
MsG0680034732.01.T01 MTR_4g021410 39.749 239 130 4 65 302 1 226 3.93e-46 155
MsG0680034732.01.T01 MTR_4g021450 38.912 239 132 3 65 302 1 226 5.98e-46 155
MsG0680034732.01.T01 MTR_4g021450 39.888 178 102 2 126 302 10 183 9.02e-41 140
MsG0680034732.01.T01 MTR_6g072710 58.036 112 47 0 174 285 1 112 1.13e-38 132
MsG0680034732.01.T01 MTR_3g092140 26.498 317 176 10 4 278 49 350 2.59e-18 85.5
MsG0680034732.01.T01 MTR_6g072705 65.957 47 16 0 89 135 38 84 7.58e-17 74.3
MsG0680034732.01.T01 MTR_3g449790 25.773 291 158 10 22 277 79 346 6.27e-13 68.9
MsG0680034732.01.T01 MTR_3g449790 27.148 291 160 12 22 277 79 352 4.54e-12 66.2
MsG0680034732.01.T01 MTR_7g114990 26.537 309 172 10 12 287 42 328 4.75e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034732.01.T01 AT1G59960 47.525 303 158 1 1 302 21 323 2.48e-106 313
MsG0680034732.01.T01 AT1G59950 47.855 303 157 1 1 302 15 317 1.08e-97 290
MsG0680034732.01.T01 AT5G62420 44.079 304 164 5 1 302 14 313 2.53e-83 254
MsG0680034732.01.T01 AT2G37770 43.617 282 155 3 22 302 34 312 7.48e-81 248
MsG0680034732.01.T01 AT2G37790 43.321 277 154 2 26 302 38 311 1.22e-80 247
MsG0680034732.01.T01 AT5G01670 39.344 305 173 6 1 302 24 319 6.93e-76 235
MsG0680034732.01.T01 AT3G53880 40.580 276 160 3 28 302 40 312 1.57e-75 234
MsG0680034732.01.T01 AT5G01670 36.145 332 173 7 1 302 24 346 8.85e-70 220
MsG0680034732.01.T01 AT2G37760 38.284 303 176 4 1 302 16 308 3.22e-69 218
MsG0680034732.01.T01 AT2G37760 38.284 303 176 4 1 302 16 308 3.22e-69 218
MsG0680034732.01.T01 AT3G53880 39.623 265 154 3 28 289 40 301 2.44e-68 215
MsG0680034732.01.T01 AT2G21250 38.889 288 160 6 1 278 11 292 1.27e-67 213
MsG0680034732.01.T01 AT2G21260 37.500 288 164 6 1 278 11 292 6.00e-67 212
MsG0680034732.01.T01 AT2G37760 38.571 280 162 3 1 280 16 285 3.45e-66 209
MsG0680034732.01.T01 AT2G37760 38.214 280 163 3 1 280 16 285 9.54e-66 208
MsG0680034732.01.T01 AT2G37760 38.214 280 163 3 1 280 16 285 9.54e-66 208
MsG0680034732.01.T01 AT2G37760 38.372 258 155 3 46 302 8 262 5.03e-58 187
MsG0680034732.01.T01 AT2G37760 38.372 258 155 3 46 302 8 262 5.03e-58 187
MsG0680034732.01.T01 AT2G37770 47.126 174 89 2 22 195 34 204 8.60e-52 172
MsG0680034732.01.T01 AT2G21250 38.865 229 124 6 1 219 11 233 2.63e-50 167
MsG0680034732.01.T01 AT2G21260 35.160 219 130 4 70 278 8 224 4.96e-46 155
MsG0680034732.01.T01 AT1G06690 28.669 293 155 11 22 278 90 364 6.48e-16 77.8
MsG0680034732.01.T01 AT5G53580 25.524 286 158 9 18 266 72 339 1.14e-14 73.9
MsG0680034732.01.T01 AT1G60750 26.645 304 181 11 15 298 39 320 3.69e-13 69.3

Find 74 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTACTTCAATGAATTT+TGG 0.132686 6:+93589931 None:intergenic
AGGTTGAGACAAGGGGTAAA+AGG 0.224517 6:-93589212 MsG0680034732.01.T01:CDS
TTTGATACTGCAGCTTATTA+TGG 0.230599 6:-93590086 MsG0680034732.01.T01:CDS
TCTATTGGTCTCAGCAATTT+TGG 0.246522 6:-93589098 MsG0680034732.01.T01:CDS
GTCTCAACCTCACTGGAAAA+TGG 0.291373 6:+93589225 None:intergenic
TATGGAACAATGTGCTAATC+TGG 0.301279 6:-93589132 MsG0680034732.01.T01:CDS
GGAGCTAAGTGAGGTTGATT+TGG 0.320507 6:-93587582 MsG0680034732.01.T01:CDS
GTGGGGTTCACTTGCTGTTA+TGG 0.330592 6:-93588305 MsG0680034732.01.T01:CDS
TGGTCCCCTTTGGGTGCTAA+TGG 0.340442 6:-93588334 MsG0680034732.01.T01:CDS
GATACAGATCCACATATTCT+AGG 0.344959 6:+93589252 None:intergenic
TAAATCTTGAAATATTTGAT+TGG 0.359973 6:-93587604 MsG0680034732.01.T01:CDS
TGTGAGTGCATGGTCCCCTT+TGG 0.386840 6:-93588344 MsG0680034732.01.T01:CDS
AAATCTTGAAATATTTGATT+GGG 0.388403 6:-93587603 MsG0680034732.01.T01:CDS
CTGGGCTTGGCTAAGTCTAT+TGG 0.410113 6:-93589113 MsG0680034732.01.T01:CDS
TTGTTGAAGCTCTTCACAAT+TGG 0.427098 6:+93587641 None:intergenic
GTGAGTGCATGGTCCCCTTT+GGG 0.428774 6:-93588343 MsG0680034732.01.T01:CDS
GAAAGGGACATGGAAAGATA+TGG 0.432757 6:-93589150 MsG0680034732.01.T01:CDS
TTTATTGCATTGATGAATGC+TGG 0.437121 6:+93590125 None:intergenic
ATTCATTCAATAAGGAGGTT+GGG 0.441247 6:-93589275 MsG0680034732.01.T01:intron
AATTTCAGGTTGCATTAAGA+TGG 0.442397 6:-93587685 MsG0680034732.01.T01:intron
TTCATCAATGCAATAAAAGT+TGG 0.457339 6:-93590119 MsG0680034732.01.T01:CDS
TTAAACAAGTATCACAATGC+AGG 0.463838 6:-93587553 MsG0680034732.01.T01:CDS
TTGTTCTAATGCTTGTGCTA+TGG 0.466766 6:+93590040 None:intergenic
AGAAAGTTCTGCAAAGAGAA+AGG 0.467312 6:-93588373 MsG0680034732.01.T01:CDS
TTCTTCCTTTCGATATGAAA+GGG 0.475077 6:-93589166 MsG0680034732.01.T01:CDS
GGGTGCTAATGGAGCACCGT+GGG 0.481143 6:-93588323 MsG0680034732.01.T01:CDS
GATTCATTCAATAAGGAGGT+TGG 0.486386 6:-93589276 MsG0680034732.01.T01:intron
AAAATGCCAATGCTAGGGTT+TGG 0.489044 6:-93590188 MsG0680034732.01.T01:CDS
ATTCTTCCTTTCGATATGAA+AGG 0.489289 6:-93589167 MsG0680034732.01.T01:CDS
GAAAGGAAGAATGTCTTCAT+TGG 0.496984 6:+93589178 None:intergenic
ACCCCTTGTCTCAACCTCAC+TGG 0.497596 6:+93589218 None:intergenic
AATTGTACCTGGACAAGAGC+AGG 0.510572 6:+93589032 None:intergenic
TATGGTTCAGAGGAACCTCT+TGG 0.512049 6:-93590068 MsG0680034732.01.T01:CDS
GTGCTCCATTAGCACCCAAA+GGG 0.512867 6:+93588329 None:intergenic
ACAATGTGCTAATCTGGGCT+TGG 0.513354 6:-93589126 MsG0680034732.01.T01:CDS
GAAAGTTCTGCAAAGAGAAA+GGG 0.525257 6:-93588372 MsG0680034732.01.T01:CDS
TATTACTCCTGCTCTTGTCC+AGG 0.527155 6:-93589039 MsG0680034732.01.T01:intron
CTTTCGATATGAAAGGGACA+TGG 0.532800 6:-93589160 MsG0680034732.01.T01:CDS
GGTTGAGACAAGGGGTAAAA+GGG 0.537900 6:-93589211 MsG0680034732.01.T01:CDS
TTTGGAGTGCAGGGAGAACA+AGG 0.539537 6:+93589949 None:intergenic
GGCCACAAAATGCCAATGCT+AGG 0.540087 6:-93590194 None:intergenic
TAAACAAGTATCACAATGCA+GGG 0.544549 6:-93587552 MsG0680034732.01.T01:CDS
GAGGTTGGGCCTAGAATATG+TGG 0.547483 6:-93589261 MsG0680034732.01.T01:CDS
AGTGCAGGGAGAACAAGGCC+AGG 0.549724 6:+93589954 None:intergenic
ATGGAACAATGTGCTAATCT+GGG 0.552590 6:-93589131 MsG0680034732.01.T01:CDS
CATGTCCCTTTCATATCGAA+AGG 0.554337 6:+93589161 None:intergenic
CCAATGCTAGGGTTTGGCAC+TGG 0.554719 6:-93590182 MsG0680034732.01.T01:CDS
ATCTCGAAAAGCTATAGCAC+AGG 0.571137 6:-93588251 MsG0680034732.01.T01:intron
TCCAGTGAGGTTGAGACAAG+GGG 0.576512 6:-93589219 MsG0680034732.01.T01:CDS
TTCCAGTGAGGTTGAGACAA+GGG 0.578167 6:-93589220 MsG0680034732.01.T01:CDS
CCAGTGCCAAACCCTAGCAT+TGG 0.583961 6:+93590182 None:intergenic
ATTTGATTGGGAGCTAAGTG+AGG 0.586529 6:-93587591 MsG0680034732.01.T01:CDS
AAGGGATTCATGTGAGTGCA+TGG 0.586898 6:-93588354 MsG0680034732.01.T01:CDS
GCCACAAAATGCCAATGCTA+GGG 0.589045 6:-93590193 None:intergenic
TGCAGCTTATTATGGTTCAG+AGG 0.598872 6:-93590078 MsG0680034732.01.T01:CDS
TGTGCTATGGCTTGACCAAG+AGG 0.599025 6:+93590053 None:intergenic
TGGTGTACTGAGGCACACCC+TGG 0.603158 6:-93589972 MsG0680034732.01.T01:CDS
CTGGAATGAGTTCATGATGT+GGG 0.604242 6:+93590144 None:intergenic
GTGCAGGGAGAACAAGGCCA+GGG 0.608494 6:+93589955 None:intergenic
TTTCCAGTGAGGTTGAGACA+AGG 0.630357 6:-93589221 MsG0680034732.01.T01:CDS
GGTGCTCCATTAGCACCCAA+AGG 0.643038 6:+93588328 None:intergenic
TGGGTGCTAATGGAGCACCG+TGG 0.645069 6:-93588324 MsG0680034732.01.T01:CDS
TTAAGATGGCTAATAGAGCA+AGG 0.653062 6:-93587671 MsG0680034732.01.T01:CDS
AATTGTGAAGAGCTTCAACA+AGG 0.674043 6:-93587639 MsG0680034732.01.T01:CDS
AAACAAGTATCACAATGCAG+GGG 0.677064 6:-93587551 MsG0680034732.01.T01:CDS
TGCTCCATTAGCACCCAAAG+GGG 0.690686 6:+93588330 None:intergenic
GGTGCTAATGGAGCACCGTG+GGG 0.692827 6:-93588322 MsG0680034732.01.T01:CDS
AGGTGGAAATGAATGCTGCA+TGG 0.695899 6:-93588411 MsG0680034732.01.T01:CDS
TGGAATGAGTTCATGATGTG+GGG 0.696997 6:+93590145 None:intergenic
ATAGCACAAGCATTAGAACA+AGG 0.704322 6:-93590038 MsG0680034732.01.T01:CDS
TACTAAACTATGGTGTACTG+AGG 0.713984 6:-93589982 MsG0680034732.01.T01:CDS
GGAATGAGTTCATGATGTGG+GGG 0.718355 6:+93590146 None:intergenic
GCTGGAATGAGTTCATGATG+TGG 0.740936 6:+93590143 None:intergenic
ATAACAGCAAGTGAACCCCA+CGG 0.751469 6:+93588307 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACACATCTTAATAT+TGG - Chr6:93588799-93588818 MsG0680034732.01.T01:intron 15.0%
!! AAATCTTGAAATATTTGATT+GGG - Chr6:93590066-93590085 MsG0680034732.01.T01:CDS 15.0%
!! AAGAAAATAGAAAATGAAAA+AGG + Chr6:93588679-93588698 None:intergenic 15.0%
!! ACAAAAGTTTACTTATAAAT+AGG + Chr6:93589880-93589899 None:intergenic 15.0%
!! ACTAAAATCAAATATTATGA+AGG - Chr6:93589622-93589641 MsG0680034732.01.T01:intron 15.0%
!! AGAAAATAGAAAATGAAAAA+GGG + Chr6:93588678-93588697 None:intergenic 15.0%
!! TAAATCTTGAAATATTTGAT+TGG - Chr6:93590065-93590084 MsG0680034732.01.T01:CDS 15.0%
!!! AAATATTTCTAATGAGTTAT+TGG + Chr6:93589223-93589242 None:intergenic 15.0%
!!! AGTTTCATATTTTAGTTTAT+AGG + Chr6:93589020-93589039 None:intergenic 15.0%
!!! ATTACTTTTAGAAAGAAAAA+AGG + Chr6:93589943-93589962 None:intergenic 15.0%
!!! TACACCTTTTTTTATAATAA+GGG + Chr6:93589726-93589745 None:intergenic 15.0%
!!! TCTACATCATTAATAATTTT+TGG - Chr6:93589650-93589669 MsG0680034732.01.T01:intron 15.0%
!!! TTACTTTTAGAAAGAAAAAA+GGG + Chr6:93589942-93589961 None:intergenic 15.0%
!! AAACTCCGAAATAAATATAT+GGG + Chr6:93589695-93589714 None:intergenic 20.0%
!! AGAAATTGAAAAGATTTCAA+TGG - Chr6:93588139-93588158 MsG0680034732.01.T01:intron 20.0%
!! ATTGGTCATAATATAAACTA+GGG - Chr6:93588817-93588836 MsG0680034732.01.T01:intron 20.0%
!! ATTTATTTCGGAGTTTATAT+TGG - Chr6:93589699-93589718 MsG0680034732.01.T01:intron 20.0%
!! GATAGTGAGATTTAAATTAA+TGG - Chr6:93588941-93588960 MsG0680034732.01.T01:intron 20.0%
!! GTACGAATTAAACAAATTAA+AGG + Chr6:93589756-93589775 None:intergenic 20.0%
!! TAAACTCCGAAATAAATATA+TGG + Chr6:93589696-93589715 None:intergenic 20.0%
!! TAAATATATGGGACAAAAAA+TGG + Chr6:93589684-93589703 None:intergenic 20.0%
!! TAAATGTGTCTCAATAAAAA+TGG - Chr6:93589057-93589076 MsG0680034732.01.T01:CDS 20.0%
!! TATTGGTCATAATATAAACT+AGG - Chr6:93588816-93588835 MsG0680034732.01.T01:intron 20.0%
!! TGAGATTTAAATTAATGGTT+GGG - Chr6:93588946-93588965 MsG0680034732.01.T01:intron 20.0%
!! TTTGTCCCATATATTTATTT+CGG - Chr6:93589687-93589706 MsG0680034732.01.T01:intron 20.0%
!!! AAATTTTCAATCACTACTTT+TGG - Chr6:93589852-93589871 MsG0680034732.01.T01:intron 20.0%
!!! AGTTTTTTAATAATCATGGA+GGG + Chr6:93587872-93587891 None:intergenic 20.0%
!!! CTACACCTTTTTTTATAATA+AGG + Chr6:93589727-93589746 None:intergenic 20.0%
!!! CTATTTTCTTTTTCATCTTT+TGG - Chr6:93588689-93588708 MsG0680034732.01.T01:intron 20.0%
!!! GGATTTATACTTATGTTTTT+TGG - Chr6:93588967-93588986 MsG0680034732.01.T01:intron 20.0%
!!! TACTTTTAGAAAGAAAAAAG+GGG + Chr6:93589941-93589960 None:intergenic 20.0%
!!! TAGTTTTTTAATAATCATGG+AGG + Chr6:93587873-93587892 None:intergenic 20.0%
!!! TTATAAAAAAAGGTGTAGAT+AGG - Chr6:93589729-93589748 MsG0680034732.01.T01:intron 20.0%
!!! TTATTAAAAAACTACGCTTT+TGG - Chr6:93587879-93587898 MsG0680034732.01.T01:intron 20.0%
!!! TTGATTACTTCAATGAATTT+TGG + Chr6:93587741-93587760 None:intergenic 20.0%
!!! TTTGCAATGTCTTTTATTAT+TGG + Chr6:93589395-93589414 None:intergenic 20.0%
! AAAAATCAAAGAATTGTACC+TGG + Chr6:93588651-93588670 None:intergenic 25.0%
! ATTTCTCTCATTCATTTCAT+TGG - Chr6:93587760-93587779 MsG0680034732.01.T01:intron 25.0%
! CAATAGAGAAAAAATAGAGA+TGG + Chr6:93588023-93588042 None:intergenic 25.0%
! CTGAAATTGAAAATGTGTAA+GGG + Chr6:93589972-93589991 None:intergenic 25.0%
! GTGAGATTTAAATTAATGGT+TGG - Chr6:93588945-93588964 MsG0680034732.01.T01:intron 25.0%
! TATATTTATAAAGCCTGATG+TGG + Chr6:93589778-93589797 None:intergenic 25.0%
! TCCAATTTCTTTGCATTATT+AGG - Chr6:93589457-93589476 MsG0680034732.01.T01:intron 25.0%
! TCCTAATAATGCAAAGAAAT+TGG + Chr6:93589461-93589480 None:intergenic 25.0%
! TGCCAAATAAAAAAATTCTC+CGG + Chr6:93588307-93588326 None:intergenic 25.0%
! TGGTCCCTTATTATAAAAAA+AGG - Chr6:93589719-93589738 MsG0680034732.01.T01:intron 25.0%
! TTCATCAATGCAATAAAAGT+TGG - Chr6:93587550-93587569 MsG0680034732.01.T01:CDS 25.0%
! TTGCATTATTAGGAATTTAG+AGG - Chr6:93589467-93589486 MsG0680034732.01.T01:intron 25.0%
!! ATCTTTTGGTAAACTATTGT+AGG - Chr6:93588703-93588722 MsG0680034732.01.T01:intron 25.0%
!! TTATTTTCTCTCGTGTTTAA+TGG + Chr6:93588057-93588076 None:intergenic 25.0%
!! TTTTTGTCACTACTAAACTA+TGG - Chr6:93587677-93587696 MsG0680034732.01.T01:CDS 25.0%
!!! AAAAAAAACTTTTTCCTGTC+CGG + Chr6:93587915-93587934 None:intergenic 25.0%
!!! ATGTGTTGATTCATTCAATA+AGG - Chr6:93588386-93588405 MsG0680034732.01.T01:CDS 25.0%
!!! GCGTAGTTTTTTAATAATCA+TGG + Chr6:93587876-93587895 None:intergenic 25.0%
!!! GTTTTTTAATAATCATGGAG+GGG + Chr6:93587871-93587890 None:intergenic 25.0%
!!! TCGTGATTTTTTCTAGAATA+GGG - Chr6:93587967-93587986 MsG0680034732.01.T01:intron 25.0%
AGAGGTTCTTATGATAAATC+AGG - Chr6:93589485-93589504 MsG0680034732.01.T01:intron 30.0%
ATAGTTAGGTTACAACTTTG+TGG - Chr6:93589572-93589591 MsG0680034732.01.T01:intron 30.0%
ATGATACAATAGGCATTTAG+CGG - Chr6:93588909-93588928 MsG0680034732.01.T01:intron 30.0%
ATTCATTCAATAAGGAGGTT+GGG - Chr6:93588394-93588413 MsG0680034732.01.T01:CDS 30.0%
ATTCTTCCTTTCGATATGAA+AGG - Chr6:93588502-93588521 MsG0680034732.01.T01:intron 30.0%
CAAATAGCTGATGATACAAT+AGG - Chr6:93588899-93588918 MsG0680034732.01.T01:intron 30.0%
CAATGGAGAGAATTCATTTA+TGG - Chr6:93588156-93588175 MsG0680034732.01.T01:intron 30.0%
CCTGAAATTGAAAATGTGTA+AGG + Chr6:93589973-93589992 None:intergenic 30.0%
GAGGTTCTTATGATAAATCA+GGG - Chr6:93589486-93589505 MsG0680034732.01.T01:intron 30.0%
TAAAAAAATTCTCCGGTCAT+AGG + Chr6:93588300-93588319 None:intergenic 30.0%
TAAACAAGTATCACAATGCA+GGG - Chr6:93590117-93590136 MsG0680034732.01.T01:CDS 30.0%
TAGGTGTTCATATCTGAATT+AGG - Chr6:93588722-93588741 MsG0680034732.01.T01:intron 30.0%
TAGTTAGGTTACAACTTTGT+GGG - Chr6:93589573-93589592 MsG0680034732.01.T01:intron 30.0%
TATTTATTGTTTGCTTTGCC+AGG - Chr6:93589238-93589257 MsG0680034732.01.T01:CDS 30.0%
TTAAACAAGTATCACAATGC+AGG - Chr6:93590116-93590135 MsG0680034732.01.T01:CDS 30.0%
TTCTTCCTTTCGATATGAAA+GGG - Chr6:93588503-93588522 MsG0680034732.01.T01:intron 30.0%
TTTGATACTGCAGCTTATTA+TGG - Chr6:93587583-93587602 MsG0680034732.01.T01:CDS 30.0%
! AACTACGCTTTTGGATTTAT+CGG - Chr6:93587888-93587907 MsG0680034732.01.T01:intron 30.0%
! CCTTACACATTTTCAATTTC+AGG - Chr6:93589970-93589989 MsG0680034732.01.T01:CDS 30.0%
! TTTATTGCATTGATGAATGC+TGG + Chr6:93587547-93587566 None:intergenic 30.0%
!! AATTTCAGGTTGCATTAAGA+TGG - Chr6:93589984-93590003 MsG0680034732.01.T01:CDS 30.0%
!! GTTTTGATGAATAAATCCAG+AGG - Chr6:93587943-93587962 MsG0680034732.01.T01:intron 30.0%
!! TGTTGATTCATTCAATAAGG+AGG - Chr6:93588389-93588408 MsG0680034732.01.T01:CDS 30.0%
!! TTTCTAGAATAGGGTAAAGT+GGG - Chr6:93587976-93587995 MsG0680034732.01.T01:intron 30.0%
!!! AGATCTTCAGTAGTTTTGTA+AGG + Chr6:93590178-93590197 None:intergenic 30.0%
!!! CTCGTGATTTTTTCTAGAAT+AGG - Chr6:93587966-93587985 MsG0680034732.01.T01:intron 30.0%
!!! GACCGGAGAATTTTTTTATT+TGG - Chr6:93588302-93588321 MsG0680034732.01.T01:CDS 30.0%
!!! TTTTCTAGAATAGGGTAAAG+TGG - Chr6:93587975-93587994 MsG0680034732.01.T01:intron 30.0%
AAACAAGTATCACAATGCAG+GGG - Chr6:93590118-93590137 MsG0680034732.01.T01:CDS 35.0%
AATTGTGAAGAGCTTCAACA+AGG - Chr6:93590030-93590049 MsG0680034732.01.T01:CDS 35.0%
AGAAAGTTCTGCAAAGAGAA+AGG - Chr6:93589296-93589315 MsG0680034732.01.T01:intron 35.0%
ATAGCACAAGCATTAGAACA+AGG - Chr6:93587631-93587650 MsG0680034732.01.T01:CDS 35.0%
ATGGAACAATGTGCTAATCT+GGG - Chr6:93588538-93588557 MsG0680034732.01.T01:intron 35.0%
GAAAGGAAGAATGTCTTCAT+TGG + Chr6:93588494-93588513 None:intergenic 35.0%
GAAAGTTCTGCAAAGAGAAA+GGG - Chr6:93589297-93589316 MsG0680034732.01.T01:intron 35.0%
GATACAGATCCACATATTCT+AGG + Chr6:93588420-93588439 None:intergenic 35.0%
GATGTTTCAATTCGTACATG+TGG - Chr6:93588266-93588285 MsG0680034732.01.T01:CDS 35.0%
GATTCATTCAATAAGGAGGT+TGG - Chr6:93588393-93588412 MsG0680034732.01.T01:CDS 35.0%
TACTAAACTATGGTGTACTG+AGG - Chr6:93587687-93587706 MsG0680034732.01.T01:intron 35.0%
TATGGAACAATGTGCTAATC+TGG - Chr6:93588537-93588556 MsG0680034732.01.T01:intron 35.0%
TGTTCAAGAATATACACGTG+TGG - Chr6:93588864-93588883 MsG0680034732.01.T01:intron 35.0%
TGTTTAATTCGTACCACATC+AGG - Chr6:93589762-93589781 MsG0680034732.01.T01:intron 35.0%
TTAAGATGGCTAATAGAGCA+AGG - Chr6:93589998-93590017 MsG0680034732.01.T01:CDS 35.0%
TTGTTCTAATGCTTGTGCTA+TGG + Chr6:93587632-93587651 None:intergenic 35.0%
TTGTTGAAGCTCTTCACAAT+TGG + Chr6:93590031-93590050 None:intergenic 35.0%
! ATCTAATCCATTTTCCAGTG+AGG - Chr6:93588437-93588456 MsG0680034732.01.T01:intron 35.0%
! ATGTGCTTGAGTTTGATGAT+TGG - Chr6:93588101-93588120 MsG0680034732.01.T01:intron 35.0%
! GAACGTTTTATCGCTGAAAA+TGG - Chr6:93590151-93590170 MsG0680034732.01.T01:CDS 35.0%
! TGTGCTTGAGTTTGATGATT+GGG - Chr6:93588102-93588121 MsG0680034732.01.T01:intron 35.0%
!! GTGACTGCTTTTACATAGTT+AGG - Chr6:93589558-93589577 MsG0680034732.01.T01:intron 35.0%
!! TCTATTGGTCTCAGCAATTT+TGG - Chr6:93588571-93588590 MsG0680034732.01.T01:intron 35.0%
!!! TCAATGAATTTTGGAGTGCA+GGG + Chr6:93587732-93587751 None:intergenic 35.0%
!!! TTCAATGAATTTTGGAGTGC+AGG + Chr6:93587733-93587752 None:intergenic 35.0%
AACATCAGAATGATGCACTC+TGG + Chr6:93588252-93588271 None:intergenic 40.0%
ATCTCGAAAAGCTATAGCAC+AGG - Chr6:93589418-93589437 MsG0680034732.01.T01:intron 40.0%
ATTTGATTGGGAGCTAAGTG+AGG - Chr6:93590078-93590097 MsG0680034732.01.T01:CDS 40.0%
CAATTCGTACATGTGGATCT+CGG - Chr6:93588273-93588292 MsG0680034732.01.T01:CDS 40.0%
CATGTCCCTTTCATATCGAA+AGG + Chr6:93588511-93588530 None:intergenic 40.0%
CTGGAATGAGTTCATGATGT+GGG + Chr6:93587528-93587547 None:intergenic 40.0%
CTTTCGATATGAAAGGGACA+TGG - Chr6:93588509-93588528 MsG0680034732.01.T01:intron 40.0%
GAAAGGGACATGGAAAGATA+TGG - Chr6:93588519-93588538 MsG0680034732.01.T01:intron 40.0%
TAGAAAAAATCACGAGCCTC+TGG + Chr6:93587962-93587981 None:intergenic 40.0%
TGCAGCTTATTATGGTTCAG+AGG - Chr6:93587591-93587610 MsG0680034732.01.T01:CDS 40.0%
TGGAATGAGTTCATGATGTG+GGG + Chr6:93587527-93587546 None:intergenic 40.0%
TTATTGTTTGCTTTGCCAGG+TGG - Chr6:93589241-93589260 MsG0680034732.01.T01:CDS 40.0%
!! TCACAATGCAGGGGTTTTAA+AGG - Chr6:93590127-93590146 MsG0680034732.01.T01:CDS 40.0%
AAGGGATTCATGTGAGTGCA+TGG - Chr6:93589315-93589334 MsG0680034732.01.T01:intron 45.0%
AATTGTACCTGGACAAGAGC+AGG + Chr6:93588640-93588659 None:intergenic 45.0%
ACAATGTGCTAATCTGGGCT+TGG - Chr6:93588543-93588562 MsG0680034732.01.T01:intron 45.0%
AGGCATTTAGCGGAATGCAT+GGG - Chr6:93588919-93588938 MsG0680034732.01.T01:intron 45.0%
AGGTGGAAATGAATGCTGCA+TGG - Chr6:93589258-93589277 MsG0680034732.01.T01:CDS 45.0%
AGGTTGAGACAAGGGGTAAA+AGG - Chr6:93588457-93588476 MsG0680034732.01.T01:intron 45.0%
AGTTCATGATGTGGGGGTAA+AGG + Chr6:93587520-93587539 None:intergenic 45.0%
ATAACAGCAAGTGAACCCCA+CGG + Chr6:93589365-93589384 None:intergenic 45.0%
ATGCAGCATTCATTTCCACC+TGG + Chr6:93589259-93589278 None:intergenic 45.0%
GCTGGAATGAGTTCATGATG+TGG + Chr6:93587529-93587548 None:intergenic 45.0%
GGAATGAGTTCATGATGTGG+GGG + Chr6:93587526-93587545 None:intergenic 45.0%
GGCTTTGCAATATGTTGCGA+AGG - Chr6:93588838-93588857 MsG0680034732.01.T01:intron 45.0%
GGTTGAGACAAGGGGTAAAA+GGG - Chr6:93588458-93588477 MsG0680034732.01.T01:intron 45.0%
GTCTCAACCTCACTGGAAAA+TGG + Chr6:93588447-93588466 None:intergenic 45.0%
TAGGCATTTAGCGGAATGCA+TGG - Chr6:93588918-93588937 MsG0680034732.01.T01:intron 45.0%
TATGGTTCAGAGGAACCTCT+TGG - Chr6:93587601-93587620 MsG0680034732.01.T01:CDS 45.0%
TATTACTCCTGCTCTTGTCC+AGG - Chr6:93588630-93588649 MsG0680034732.01.T01:intron 45.0%
TTCCAGTGAGGTTGAGACAA+GGG - Chr6:93588449-93588468 MsG0680034732.01.T01:intron 45.0%
TTTCCAGTGAGGTTGAGACA+AGG - Chr6:93588448-93588467 MsG0680034732.01.T01:intron 45.0%
! CGCTTTTGGATTTATCGGTC+CGG - Chr6:93587893-93587912 MsG0680034732.01.T01:intron 45.0%
! GGAGCTAAGTGAGGTTGATT+TGG - Chr6:93590087-93590106 MsG0680034732.01.T01:CDS 45.0%
!! TTTATAATTCTATATATAAA+TGG + Chr6:93589110-93589129 None:intergenic 5.0%
CTGGGCTTGGCTAAGTCTAT+TGG - Chr6:93588556-93588575 MsG0680034732.01.T01:intron 50.0%
GAGGTTGGGCCTAGAATATG+TGG - Chr6:93588408-93588427 MsG0680034732.01.T01:CDS 50.0%
GTGCTCCATTAGCACCCAAA+GGG + Chr6:93589343-93589362 None:intergenic 50.0%
TCCAGTGAGGTTGAGACAAG+GGG - Chr6:93588450-93588469 MsG0680034732.01.T01:intron 50.0%
TGCTCCATTAGCACCCAAAG+GGG + Chr6:93589342-93589361 None:intergenic 50.0%
TGTGCTATGGCTTGACCAAG+AGG + Chr6:93587619-93587638 None:intergenic 50.0%
TTGGATTTATCGGTCCGGAC+AGG - Chr6:93587898-93587917 MsG0680034732.01.T01:intron 50.0%
! TTTGGAGTGCAGGGAGAACA+AGG + Chr6:93587723-93587742 None:intergenic 50.0%
!! GTGGGGTTCACTTGCTGTTA+TGG - Chr6:93589364-93589383 MsG0680034732.01.T01:intron 50.0%
ACCCCTTGTCTCAACCTCAC+TGG + Chr6:93588454-93588473 None:intergenic 55.0%
CCAATGCTAGGGTTTGGCAC+TGG - Chr6:93587487-93587506 MsG0680034732.01.T01:CDS 55.0%
CCAGTGCCAAACCCTAGCAT+TGG + Chr6:93587490-93587509 None:intergenic 55.0%
GGTGCTCCATTAGCACCCAA+AGG + Chr6:93589344-93589363 None:intergenic 55.0%
GTGAGTGCATGGTCCCCTTT+GGG - Chr6:93589326-93589345 MsG0680034732.01.T01:intron 55.0%
GTGGATCTCGGACCTATGAC+CGG - Chr6:93588285-93588304 MsG0680034732.01.T01:CDS 55.0%
TGGTCCCCTTTGGGTGCTAA+TGG - Chr6:93589335-93589354 MsG0680034732.01.T01:intron 55.0%
TGTGAGTGCATGGTCCCCTT+TGG - Chr6:93589325-93589344 MsG0680034732.01.T01:intron 55.0%
AGTGCAGGGAGAACAAGGCC+AGG + Chr6:93587718-93587737 None:intergenic 60.0%
GTGCAGGGAGAACAAGGCCA+GGG + Chr6:93587717-93587736 None:intergenic 60.0%
TGGTGTACTGAGGCACACCC+TGG - Chr6:93587697-93587716 MsG0680034732.01.T01:intron 60.0%
!! GGGTGCTAATGGAGCACCGT+GGG - Chr6:93589346-93589365 MsG0680034732.01.T01:intron 60.0%
!! GGTGCTAATGGAGCACCGTG+GGG - Chr6:93589347-93589366 MsG0680034732.01.T01:intron 60.0%
!! TGGGTGCTAATGGAGCACCG+TGG - Chr6:93589345-93589364 MsG0680034732.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 93587484 93590207 93587484 ID=MsG0680034732.01;Name=MsG0680034732.01
Chr6 mRNA 93587484 93590207 93587484 ID=MsG0680034732.01.T01;Parent=MsG0680034732.01;Name=MsG0680034732.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|0.75|4|0|302
Chr6 exon 93589939 93590207 93589939 ID=MsG0680034732.01.T01:exon:5779;Parent=MsG0680034732.01.T01
Chr6 exon 93589040 93589283 93589040 ID=MsG0680034732.01.T01:exon:5778;Parent=MsG0680034732.01.T01
Chr6 exon 93588252 93588431 93588252 ID=MsG0680034732.01.T01:exon:5777;Parent=MsG0680034732.01.T01
Chr6 exon 93587484 93587699 93587484 ID=MsG0680034732.01.T01:exon:5776;Parent=MsG0680034732.01.T01
Chr6 CDS 93589939 93590207 93589939 ID=MsG0680034732.01.T01:cds;Parent=MsG0680034732.01.T01
Chr6 CDS 93589040 93589283 93589040 ID=MsG0680034732.01.T01:cds;Parent=MsG0680034732.01.T01
Chr6 CDS 93588252 93588431 93588252 ID=MsG0680034732.01.T01:cds;Parent=MsG0680034732.01.T01
Chr6 CDS 93587484 93587699 93587484 ID=MsG0680034732.01.T01:cds;Parent=MsG0680034732.01.T01
Gene Sequence

>MsG0680034732.01.T01

ATGCCAATGCTAGGGTTTGGCACTGGAACAGTACCTTTACCCCCACATCATGAACTCATTCCAGCATTCATCAATGCAATAAAAGTTGGCTATAGACATTTTGATACTGCAGCTTATTATGGTTCAGAGGAACCTCTTGGTCAAGCCATAGCACAAGCATTAGAACAAGGTCTTATTAAAAAACGTAGTGACATTTTTGTCACTACTAAACTATGGTGTACTGAGGCACACCCTGGCCTTGTTCTCCCTGCACTCCAAAATTCATTGAAGAGGTTGGGCCTAGAATATGTGGATCTGTATCTAATCCATTTTCCAGTGAGGTTGAGACAAGGGGTAAAAGGGATCAACTACACCAATGAAGACATTCTTCCTTTCGATATGAAAGGGACATGGAAAGATATGGAACAATGTGCTAATCTGGGCTTGGCTAAGTCTATTGGTCTCAGCAATTTTGGTGTGAAAAAAATTTCAGAAATTCTAGAATATGCAACTATTACTCCTGCTCTTGTCCAGGTGGAAATGAATGCTGCATGGCATCAAGAAAATCTGAGAAAGTTCTGCAAAGAGAAAGGGATTCATGTGAGTGCATGGTCCCCTTTGGGTGCTAATGGAGCACCGTGGGGTTCACTTGCTGTTATGGACAATCCAATAATAAAAGACATTGCAAATTCATCTCGAAAAGCTATAGCACAGGTTGCATTAAGATGGCTAATAGAGCAAGGTGCTACTCCAATTGTGAAGAGCTTCAACAAGGAGAGAATGAAAATAAATCTTGAAATATTTGATTGGGAGCTAAGTGAGGTTGATTTGGAGAAGATTAAACAAGTATCACAATGCAGGGGTTTTAAAGGAGAACGTTTTATCGCTGAAAATGGACCTTACAAAACTACTGAAGATCTTTTTGACTGA
Protein sequence

>MsG0680034732.01.T01

MPMLGFGTGTVPLPPHHELIPAFINAIKVGYRHFDTAAYYGSEEPLGQAIAQALEQGLIKKRSDIFVTTKLWCTEAHPGLVLPALQNSLKRLGLEYVDLYLIHFPVRLRQGVKGINYTNEDILPFDMKGTWKDMEQCANLGLAKSIGLSNFGVKKISEILEYATITPALVQVEMNAAWHQENLRKFCKEKGIHVSAWSPLGANGAPWGSLAVMDNPIIKDIANSSRKAIAQVALRWLIEQGATPIVKSFNKERMKINLEIFDWELSEVDLEKIKQVSQCRGFKGERFIAENGPYKTTEDLFD*