AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019714.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019714.01.T01 MTR_4g036845 97.778 315 7 0 1 315 1 315 0.0 648
MsG0480019714.01.T01 MTR_4g072320 46.057 317 162 5 2 315 5 315 1.82e-95 285
MsG0480019714.01.T01 MTR_4g072350 45.110 317 169 5 2 314 5 320 3.81e-89 269
MsG0480019714.01.T01 MTR_6g073110 45.338 311 165 4 6 312 9 318 5.13e-89 269
MsG0480019714.01.T01 MTR_8g070095 42.628 312 173 3 8 315 10 319 1.29e-86 263
MsG0480019714.01.T01 MTR_4g072360 44.444 315 170 5 2 312 5 318 3.99e-86 261
MsG0480019714.01.T01 MTR_0374s0050 44.118 306 156 6 14 314 20 315 1.51e-85 260
MsG0480019714.01.T01 MTR_8g070115 42.320 319 173 5 3 315 2 315 1.35e-84 258
MsG0480019714.01.T01 MTR_7g063580 40.498 321 170 5 4 315 46 354 1.84e-77 241
MsG0480019714.01.T01 MTR_4g021350 41.158 311 175 4 7 315 9 313 6.93e-76 235
MsG0480019714.01.T01 MTR_3g083130 40.000 305 169 7 14 314 21 315 1.95e-75 234
MsG0480019714.01.T01 MTR_4g021410 40.323 310 166 5 8 314 11 304 1.86e-68 216
MsG0480019714.01.T01 MTR_4g021450 39.677 310 168 5 8 314 11 304 5.76e-67 212
MsG0480019714.01.T01 MTR_4g021350 42.391 276 150 5 7 280 9 277 1.93e-65 207
MsG0480019714.01.T01 MTR_5g097910 39.672 305 171 6 12 313 18 312 6.84e-65 207
MsG0480019714.01.T01 MTR_5g097940 39.672 305 171 6 12 313 18 312 6.84e-65 207
MsG0480019714.01.T01 MTR_5g097900 40.351 285 165 4 12 293 18 300 1.25e-64 206
MsG0480019714.01.T01 MTR_4g021410 41.367 278 148 3 39 314 3 267 3.09e-64 204
MsG0480019714.01.T01 MTR_7g070500 38.782 312 178 8 8 314 17 320 1.54e-61 199
MsG0480019714.01.T01 MTR_4g072060 37.500 304 172 5 6 299 3 298 2.74e-59 192
MsG0480019714.01.T01 MTR_7g070500 39.502 281 159 6 8 285 17 289 3.97e-54 179
MsG0480019714.01.T01 MTR_4g021410 40.249 241 129 3 76 314 1 228 1.63e-49 164
MsG0480019714.01.T01 MTR_4g021450 40.083 242 128 4 76 314 1 228 5.67e-49 163
MsG0480019714.01.T01 MTR_4g021450 41.989 181 99 2 135 314 10 185 5.26e-45 152
MsG0480019714.01.T01 MTR_6g072650 38.776 147 67 2 168 314 13 136 1.04e-27 105
MsG0480019714.01.T01 MTR_6g072710 44.248 113 63 0 183 295 1 113 3.62e-25 97.8
MsG0480019714.01.T01 MTR_3g449790 25.460 326 172 12 12 285 45 351 8.27e-18 83.2
MsG0480019714.01.T01 MTR_3g449790 24.540 326 169 11 12 285 45 345 3.60e-17 81.6
MsG0480019714.01.T01 MTR_7g114980 26.398 322 172 12 20 298 35 334 3.79e-16 78.6
MsG0480019714.01.T01 MTR_1g056790 94.118 34 2 0 263 296 1 34 2.03e-14 67.0
MsG0480019714.01.T01 MTR_7g114990 26.000 300 163 10 20 282 37 314 4.60e-14 72.4
MsG0480019714.01.T01 MTR_1g102750 24.776 335 174 12 11 294 14 321 1.95e-13 70.5
MsG0480019714.01.T01 MTR_7g021850 25.688 327 168 14 11 288 13 313 7.21e-13 68.6
MsG0480019714.01.T01 MTR_7g021680 24.923 325 170 13 11 287 13 311 4.67e-11 63.2
MsG0480019714.01.T01 MTR_7g021820 24.923 325 170 13 11 287 13 311 4.67e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019714.01.T01 AT5G62420 67.405 316 102 1 1 315 1 316 2.77e-163 457
MsG0480019714.01.T01 AT2G37770 44.373 311 167 3 7 315 9 315 2.89e-91 275
MsG0480019714.01.T01 AT2G37790 43.087 311 168 5 8 315 10 314 6.56e-85 258
MsG0480019714.01.T01 AT1G59960 41.748 309 172 7 13 315 20 326 1.23e-84 258
MsG0480019714.01.T01 AT2G37760 41.290 310 172 3 8 315 10 311 8.68e-81 248
MsG0480019714.01.T01 AT2G37760 41.290 310 172 3 8 315 10 311 8.68e-81 248
MsG0480019714.01.T01 AT3G53880 41.158 311 177 3 7 315 9 315 5.61e-79 243
MsG0480019714.01.T01 AT1G59950 41.234 308 173 7 14 315 15 320 6.65e-75 233
MsG0480019714.01.T01 AT2G37760 40.210 286 161 3 8 291 10 287 2.26e-73 228
MsG0480019714.01.T01 AT2G37760 40.210 286 161 3 8 291 10 287 2.26e-73 228
MsG0480019714.01.T01 AT2G37760 42.545 275 147 4 8 280 10 275 3.63e-73 228
MsG0480019714.01.T01 AT5G01670 39.103 312 179 7 7 314 17 321 1.56e-71 224
MsG0480019714.01.T01 AT2G21250 38.487 304 169 6 6 299 3 298 2.80e-69 218
MsG0480019714.01.T01 AT2G21260 38.033 305 171 6 6 300 3 299 1.36e-68 216
MsG0480019714.01.T01 AT5G01670 36.283 339 178 8 7 314 17 348 3.24e-67 214
MsG0480019714.01.T01 AT3G53880 39.789 284 165 3 7 288 9 288 1.80e-66 211
MsG0480019714.01.T01 AT2G37760 41.538 260 148 2 58 315 8 265 5.45e-65 206
MsG0480019714.01.T01 AT2G37760 41.538 260 148 2 58 315 8 265 5.45e-65 206
MsG0480019714.01.T01 AT2G21250 38.627 233 131 5 6 228 3 233 2.46e-52 172
MsG0480019714.01.T01 AT2G37770 43.000 200 108 3 7 204 9 204 8.80e-51 170
MsG0480019714.01.T01 AT2G21260 37.768 233 129 5 78 300 5 231 1.20e-46 157
MsG0480019714.01.T01 AT1G06690 26.471 306 165 11 29 293 85 371 4.00e-19 87.4
MsG0480019714.01.T01 AT1G60710 25.705 319 170 11 22 298 33 326 7.79e-14 71.6
MsG0480019714.01.T01 AT5G53580 24.038 312 178 8 17 283 50 347 2.88e-13 70.1
MsG0480019714.01.T01 AT1G60690 24.566 346 185 14 2 297 6 325 2.75e-12 67.0
MsG0480019714.01.T01 AT1G60750 24.601 313 184 12 22 308 34 320 4.60e-12 66.2
MsG0480019714.01.T01 AT1G60730 24.611 321 179 11 22 302 33 330 7.42e-12 65.9
MsG0480019714.01.T01 AT1G60730 24.620 329 189 12 22 302 33 350 9.56e-12 65.5

Find 78 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATATAGATTGCCTTATTTA+TGG 0.094666 4:+25660888 None:intergenic
TGAAGATGACTTTGAAAATT+TGG 0.094815 4:-25660513 MsG0480019714.01.T01:CDS
AAGGCAATTGATTGTATAAT+TGG 0.200651 4:+25659844 None:intergenic
GAATCTGGACATGGAATATT+TGG 0.203266 4:-25660597 MsG0480019714.01.T01:CDS
TCTGTTGTCTTCTTATCATT+TGG 0.211759 4:+25661490 None:intergenic
CATAAATAAGGCAATCTATA+TGG 0.224825 4:-25660887 MsG0480019714.01.T01:CDS
TGAAAGAATGGAGGAAAATA+TGG 0.239865 4:-25659418 MsG0480019714.01.T01:CDS
CTGTTGTCTTCTTATCATTT+GGG 0.296041 4:+25661491 None:intergenic
ACAATGCCTCTCATTGGATT+TGG 0.330693 4:-25661526 MsG0480019714.01.T01:CDS
TGATATTTAATTACCTGTGC+TGG 0.338494 4:+25659805 None:intergenic
ACAAAACAATTGAAGAACTT+TGG 0.349387 4:-25659287 MsG0480019714.01.T01:CDS
ATTAGGCAATGCCATAAATA+AGG 0.350442 4:-25660899 MsG0480019714.01.T01:CDS
CAAAACAATTGAAGAACTTT+GGG 0.354545 4:-25659286 MsG0480019714.01.T01:CDS
ATTTGAAGGTTGCATTGAAA+TGG 0.361915 4:-25659485 MsG0480019714.01.T01:intron
TGGGGTTGCTTTGTGTTTGA+AGG 0.388559 4:+25659825 None:intergenic
GGCATTACAATGCCTCTCAT+TGG 0.395160 4:-25661532 MsG0480019714.01.T01:CDS
TACACCTCCAGTTGTTAATC+AGG 0.396062 4:-25660375 MsG0480019714.01.T01:intron
GTTTGTTTCAATGCAGCAAC+AGG 0.398249 4:+25660802 None:intergenic
TTTGAAGGTTGCATTGAAAT+GGG 0.414907 4:-25659484 MsG0480019714.01.T01:intron
GATATTTAATTACCTGTGCT+GGG 0.417731 4:+25659806 None:intergenic
TTAATTACCTGATTAACAAC+TGG 0.420192 4:+25660368 None:intergenic
TACTCACCACTTGGTGGACC+AGG 0.421010 4:-25659901 MsG0480019714.01.T01:CDS
AAGTCATCTTCATTAGGAAC+AGG 0.433658 4:+25660524 None:intergenic
ATAAATAAGGCAATCTATAT+GGG 0.442614 4:-25660886 MsG0480019714.01.T01:CDS
TTTATGGCATTGCCTAATGC+TGG 0.448486 4:+25660904 None:intergenic
TTGGTGGACCAGGGAATGCA+TGG 0.463271 4:-25659891 MsG0480019714.01.T01:CDS
TATGGCTCTGAGCCAGCATT+AGG 0.474356 4:-25660916 MsG0480019714.01.T01:CDS
GTTGATCCCCATGCATTCCC+TGG 0.477901 4:+25659883 None:intergenic
TTTCTTGACATCCAAATTGT+GGG 0.481755 4:-25660842 MsG0480019714.01.T01:CDS
GCCTGTAAAGTTAAAACCAT+GGG 0.488120 4:-25660555 MsG0480019714.01.T01:CDS
TGGATATGTACCTTGTGCAT+TGG 0.492094 4:-25660577 MsG0480019714.01.T01:CDS
TCTATATGGGAGAAGTAGAA+AGG 0.493846 4:-25660873 MsG0480019714.01.T01:CDS
GAAAGAATGGAGGAAAATAT+GGG 0.502628 4:-25659417 MsG0480019714.01.T01:CDS
GGGATCATTTGATCTAAACT+TGG 0.506454 4:-25659397 MsG0480019714.01.T01:CDS
GTCTTGAAATGGGGTTATGT+AGG 0.513056 4:-25660457 MsG0480019714.01.T01:CDS
AAGTTTCAATGATGAAAGAA+TGG 0.517652 4:-25659430 MsG0480019714.01.T01:CDS
TGTCTTCTTATCATTTGGGT+AGG 0.528221 4:+25661495 None:intergenic
ATGGGGTTATGTAGGTCCAT+TGG 0.529847 4:-25660449 MsG0480019714.01.T01:CDS
CTATATGGGAGAAGTAGAAA+GGG 0.533515 4:-25660872 MsG0480019714.01.T01:CDS
ATTTGGATCTTGAGACAACA+TGG 0.540374 4:-25660496 MsG0480019714.01.T01:CDS
AATCATGTGCGTTTGAATTG+TGG 0.556437 4:-25661553 MsG0480019714.01.T01:CDS
TCAATGCAGCAACAGGATCA+TGG 0.559666 4:+25660809 None:intergenic
TGGTGGACCAGGGAATGCAT+GGG 0.560104 4:-25659890 MsG0480019714.01.T01:CDS
TTGAAATGGGGATTATCAAA+GGG 0.560330 4:-25659471 MsG0480019714.01.T01:CDS
ATTGAAATGGGGATTATCAA+AGG 0.560489 4:-25659472 MsG0480019714.01.T01:CDS
AAGAAATGAAGATTATGAGA+GGG 0.564196 4:-25659341 MsG0480019714.01.T01:CDS
TGAGACAACATGGGTAGGGA+TGG 0.572572 4:-25660486 MsG0480019714.01.T01:CDS
TTCTTGACATCCAAATTGTG+GGG 0.578334 4:-25660841 MsG0480019714.01.T01:CDS
GGAACAGGATAGTTAACCCA+TGG 0.578853 4:+25660539 None:intergenic
TGGTCCACCAAGTGGTGAGT+AGG 0.583500 4:+25659903 None:intergenic
GATCTTGAGACAACATGGGT+AGG 0.585515 4:-25660491 MsG0480019714.01.T01:CDS
ACACAAAGCAACCCCAGCAC+AGG 0.586444 4:-25659818 MsG0480019714.01.T01:intron
TTGAAGGTTGCATTGAAATG+GGG 0.588735 4:-25659483 MsG0480019714.01.T01:intron
GAAGAAATGAAGATTATGAG+AGG 0.591725 4:-25659342 MsG0480019714.01.T01:CDS
AGAGAAATTACTAACTCCAA+TGG 0.591742 4:+25660433 None:intergenic
ATCTTGAGACAACATGGGTA+GGG 0.592828 4:-25660490 MsG0480019714.01.T01:CDS
TGCAGTCCACAATGCCCTTG+AGG 0.593505 4:-25661464 MsG0480019714.01.T01:intron
TCTCTAGCAACAAGATTGAG+TGG 0.593774 4:-25660415 MsG0480019714.01.T01:CDS
TTTCAATGATGAAAGAATGG+AGG 0.594754 4:-25659427 MsG0480019714.01.T01:CDS
GGCCTGTAAAGTTAAAACCA+TGG 0.600838 4:-25660556 MsG0480019714.01.T01:CDS
ATATTTAATTACCTGTGCTG+GGG 0.601478 4:+25659807 None:intergenic
AGAAATGAAGATTATGAGAG+GGG 0.602586 4:-25659340 MsG0480019714.01.T01:CDS
ACTTTACAGGCCAATGCACA+AGG 0.616222 4:+25660567 None:intergenic
GTAAGTGCCTACTCACCACT+TGG 0.619442 4:-25659910 MsG0480019714.01.T01:CDS
AGTGCCTACTCACCACTTGG+TGG 0.619529 4:-25659907 MsG0480019714.01.T01:CDS
AGCTTTCTCTGCCTCCACAT+AGG 0.620551 4:+25659964 None:intergenic
TCTTGACATCCAAATTGTGG+GGG 0.621026 4:-25660840 MsG0480019714.01.T01:CDS
AGGTGGAAATGCATCCTATG+TGG 0.626109 4:-25659978 MsG0480019714.01.T01:CDS
ATTACCTGATTAACAACTGG+AGG 0.626966 4:+25660371 None:intergenic
AGAAAGCTGAGAAAGACATG+TGG 0.633349 4:-25659949 MsG0480019714.01.T01:CDS
GATTAAGGTTAGTACCTCAA+GGG 0.643115 4:+25661450 None:intergenic
TAAGTGCCAAATCCAATGAG+AGG 0.655261 4:+25661520 None:intergenic
TGGAAATGCATCCTATGTGG+AGG 0.657202 4:-25659975 MsG0480019714.01.T01:CDS
GGTGGACCAGGGAATGCATG+GGG 0.659494 4:-25659889 MsG0480019714.01.T01:CDS
GCATTCCCTGGTCCACCAAG+TGG 0.660549 4:+25659895 None:intergenic
TTTGGATCTTGAGACAACAT+GGG 0.665864 4:-25660495 MsG0480019714.01.T01:CDS
ACTCACCACTTGGTGGACCA+GGG 0.670084 4:-25659900 MsG0480019714.01.T01:CDS
TTAGTACCTCAAGGGCATTG+TGG 0.678400 4:+25661458 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAAAAAATTAAGATAT+AGG + Chr4:25660106-25660125 None:intergenic 10.0%
!!! ATATATTTTATTTACTTGTT+TGG + Chr4:25661102-25661121 None:intergenic 10.0%
!!! CTATATTAATTTAAAATACA+AGG + Chr4:25659760-25659779 None:intergenic 10.0%
!!! TAATATTTTGATATATACTT+AGG - Chr4:25660836-25660855 MsG0480019714.01.T01:CDS 10.0%
!! TATATTTGTGTTCAATAATA+AGG + Chr4:25659848-25659867 None:intergenic 15.0%
!! TATCAAATTTATTTGAACAT+TGG + Chr4:25661210-25661229 None:intergenic 15.0%
!! TTATTAAATCAACCTAAAAA+TGG + Chr4:25660548-25660567 None:intergenic 15.0%
!!! TATAAGAAAAATCCATTTTT+AGG - Chr4:25660533-25660552 MsG0480019714.01.T01:CDS 15.0%
!!! TATATTTGAGATTGAAAAAT+TGG - Chr4:25661470-25661489 MsG0480019714.01.T01:CDS 15.0%
!! AAAGATATAGATCAATTTGA+TGG - Chr4:25659670-25659689 MsG0480019714.01.T01:intron 20.0%
!! ACTGAATGAAATAACTTATT+TGG - Chr4:25660650-25660669 MsG0480019714.01.T01:intron 20.0%
!! ATAAATAAGGCAATCTATAT+GGG - Chr4:25659948-25659967 MsG0480019714.01.T01:CDS 20.0%
!! CTGAATGAAATAACTTATTT+GGG - Chr4:25660651-25660670 MsG0480019714.01.T01:intron 20.0%
!! TAATCAGGTAATTAACTAAT+TGG - Chr4:25660474-25660493 MsG0480019714.01.T01:CDS 20.0%
!! TTGAAATATTAATAGAGTCA+AGG - Chr4:25659626-25659645 MsG0480019714.01.T01:intron 20.0%
!! TTTGATACAGCAAAAATTTA+TGG - Chr4:25659900-25659919 MsG0480019714.01.T01:CDS 20.0%
!!! CAAGTTTAAGACTTTTTTTT+TGG - Chr4:25661050-25661069 MsG0480019714.01.T01:intron 20.0%
!!! TATTTTGATATATACTTAGG+TGG - Chr4:25660839-25660858 MsG0480019714.01.T01:CDS 20.0%
! AACAAATGTGTATGTGATTA+AGG + Chr4:25659402-25659421 None:intergenic 25.0%
! AAGAAATGAAGATTATGAGA+GGG - Chr4:25661493-25661512 MsG0480019714.01.T01:CDS 25.0%
! AAGGCAATTGATTGTATAAT+TGG + Chr4:25660993-25661012 None:intergenic 25.0%
! AAGTTTCAATGATGAAAGAA+TGG - Chr4:25661404-25661423 MsG0480019714.01.T01:intron 25.0%
! AATTGAAAGAATGTAACATG+TGG - Chr4:25660150-25660169 MsG0480019714.01.T01:intron 25.0%
! ACAAAACAATTGAAGAACTT+TGG - Chr4:25661547-25661566 MsG0480019714.01.T01:CDS 25.0%
! ATATATGTATGTGTGTATAC+AGG - Chr4:25659863-25659882 MsG0480019714.01.T01:CDS 25.0%
! ATGGAAAAATGTCTTGAAAT+GGG - Chr4:25660367-25660386 MsG0480019714.01.T01:intron 25.0%
! CAAAACAATTGAAGAACTTT+GGG - Chr4:25661548-25661567 MsG0480019714.01.T01:CDS 25.0%
! CATAAATAAGGCAATCTATA+TGG - Chr4:25659947-25659966 MsG0480019714.01.T01:CDS 25.0%
! TAATTGGAATTTATCTTGAG+TGG - Chr4:25660490-25660509 MsG0480019714.01.T01:CDS 25.0%
! TCAAACTAATTTACTGATGT+TGG + Chr4:25660750-25660769 None:intergenic 25.0%
! TGTATCCTTAGTAAAATGAA+TGG - Chr4:25661281-25661300 MsG0480019714.01.T01:intron 25.0%
! TTAATTACCTGATTAACAAC+TGG + Chr4:25660469-25660488 None:intergenic 25.0%
! TTGTCAAAAGTTTCTCAATT+AGG + Chr4:25659592-25659611 None:intergenic 25.0%
!! CATTACCATTCATTTTACTA+AGG + Chr4:25661289-25661308 None:intergenic 25.0%
!! TGAAGATGACTTTGAAAATT+TGG - Chr4:25660321-25660340 MsG0480019714.01.T01:intron 25.0%
!! TTTTCAAAGTCATCTTCATT+AGG + Chr4:25660319-25660338 None:intergenic 25.0%
!!! AAGTTCTTCAATTGTTTTGT+AGG + Chr4:25661547-25661566 None:intergenic 25.0%
!!! AGTTCTTCAATTGTTTTGTA+GGG + Chr4:25661546-25661565 None:intergenic 25.0%
!!! CATATAGATTGCCTTATTTA+TGG + Chr4:25659949-25659968 None:intergenic 25.0%
!!! CTTTTTGTTTTTGTCAAAGA+CGG + Chr4:25659562-25659581 None:intergenic 25.0%
!!! GATTTTTTGAATGCAAAAAC+AGG - Chr4:25660215-25660234 MsG0480019714.01.T01:intron 25.0%
AGAAATGAAGATTATGAGAG+GGG - Chr4:25661494-25661513 MsG0480019714.01.T01:CDS 30.0%
AGAGAAATTACTAACTCCAA+TGG + Chr4:25660404-25660423 None:intergenic 30.0%
ATAAGCAATGCAAAAACATG+TGG - Chr4:25660180-25660199 MsG0480019714.01.T01:intron 30.0%
ATATTTAATTACCTGTGCTG+GGG + Chr4:25661030-25661049 None:intergenic 30.0%
ATTAGGCAATGCCATAAATA+AGG - Chr4:25659935-25659954 MsG0480019714.01.T01:CDS 30.0%
ATTGAAATGGGGATTATCAA+AGG - Chr4:25661362-25661381 MsG0480019714.01.T01:intron 30.0%
ATTTACTGATGTTGGTATCA+AGG + Chr4:25660742-25660761 None:intergenic 30.0%
ATTTGAAGGTTGCATTGAAA+TGG - Chr4:25661349-25661368 MsG0480019714.01.T01:intron 30.0%
CAAAGACAAGACCAATTTAA+TGG + Chr4:25659488-25659507 None:intergenic 30.0%
CGTCTTTGACAAAAACAAAA+AGG - Chr4:25659560-25659579 MsG0480019714.01.T01:intron 30.0%
CTGTTGTCTTCTTATCATTT+GGG + Chr4:25659346-25659365 None:intergenic 30.0%
GAAAGAATGGAGGAAAATAT+GGG - Chr4:25661417-25661436 MsG0480019714.01.T01:intron 30.0%
GAAGAAATGAAGATTATGAG+AGG - Chr4:25661492-25661511 MsG0480019714.01.T01:CDS 30.0%
GATATTTAATTACCTGTGCT+GGG + Chr4:25661031-25661050 None:intergenic 30.0%
GATGGAAAAATGTCTTGAAA+TGG - Chr4:25660366-25660385 MsG0480019714.01.T01:intron 30.0%
GGATACAAATGCAACTAATT+TGG + Chr4:25661268-25661287 None:intergenic 30.0%
TAGCGAGTAAACCATTAAAT+TGG - Chr4:25659474-25659493 MsG0480019714.01.T01:CDS 30.0%
TCTGTTGTCTTCTTATCATT+TGG + Chr4:25659347-25659366 None:intergenic 30.0%
TGAAAGAATGGAGGAAAATA+TGG - Chr4:25661416-25661435 MsG0480019714.01.T01:intron 30.0%
TGATATTTAATTACCTGTGC+TGG + Chr4:25661032-25661051 None:intergenic 30.0%
TGGAAAAATGTCTTGAAATG+GGG - Chr4:25660368-25660387 MsG0480019714.01.T01:intron 30.0%
TTGAAATGGGGATTATCAAA+GGG - Chr4:25661363-25661382 MsG0480019714.01.T01:intron 30.0%
TTGACAAAAACAAAAAGGCA+GGG - Chr4:25659565-25659584 MsG0480019714.01.T01:intron 30.0%
TTTCAATGATGAAAGAATGG+AGG - Chr4:25661407-25661426 MsG0480019714.01.T01:intron 30.0%
TTTCTTGACATCCAAATTGT+GGG - Chr4:25659992-25660011 MsG0480019714.01.T01:intron 30.0%
TTTGAAGGTTGCATTGAAAT+GGG - Chr4:25661350-25661369 MsG0480019714.01.T01:intron 30.0%
TTTGACAAAAACAAAAAGGC+AGG - Chr4:25659564-25659583 MsG0480019714.01.T01:intron 30.0%
! TTTTCTTGACATCCAAATTG+TGG - Chr4:25659991-25660010 MsG0480019714.01.T01:intron 30.0%
!! GACTATGATCTTTGATTTGA+AGG - Chr4:25661335-25661354 MsG0480019714.01.T01:intron 30.0%
AAGTCATCTTCATTAGGAAC+AGG + Chr4:25660313-25660332 None:intergenic 35.0%
AATCATGTGCGTTTGAATTG+TGG - Chr4:25659281-25659300 MsG0480019714.01.T01:CDS 35.0%
ATGTATGTGTGTATACAGGT+GGG - Chr4:25659867-25659886 MsG0480019714.01.T01:CDS 35.0%
ATTACCTGATTAACAACTGG+AGG + Chr4:25660466-25660485 None:intergenic 35.0%
ATTTGGATCTTGAGACAACA+TGG - Chr4:25660338-25660357 MsG0480019714.01.T01:intron 35.0%
CTATATGGGAGAAGTAGAAA+GGG - Chr4:25659962-25659981 MsG0480019714.01.T01:CDS 35.0%
GAATCTGGACATGGAATATT+TGG - Chr4:25660237-25660256 MsG0480019714.01.T01:intron 35.0%
GATTAAGGTTAGTACCTCAA+GGG + Chr4:25659387-25659406 None:intergenic 35.0%
GCCTGTAAAGTTAAAACCAT+GGG - Chr4:25660279-25660298 MsG0480019714.01.T01:intron 35.0%
GGGATCATTTGATCTAAACT+TGG - Chr4:25661437-25661456 MsG0480019714.01.T01:intron 35.0%
TATGTATGTGTGTATACAGG+TGG - Chr4:25659866-25659885 MsG0480019714.01.T01:CDS 35.0%
TCTATATGGGAGAAGTAGAA+AGG - Chr4:25659961-25659980 MsG0480019714.01.T01:CDS 35.0%
TGAATGCAAAAACAGGAATC+TGG - Chr4:25660222-25660241 MsG0480019714.01.T01:intron 35.0%
TGATTAAGGTTAGTACCTCA+AGG + Chr4:25659388-25659407 None:intergenic 35.0%
TGTCTTCTTATCATTTGGGT+AGG + Chr4:25659342-25659361 None:intergenic 35.0%
TTCTTGACATCCAAATTGTG+GGG - Chr4:25659993-25660012 MsG0480019714.01.T01:intron 35.0%
TTGAAGGTTGCATTGAAATG+GGG - Chr4:25661351-25661370 MsG0480019714.01.T01:intron 35.0%
TTTGGATCTTGAGACAACAT+GGG - Chr4:25660339-25660358 MsG0480019714.01.T01:intron 35.0%
!! AATTGTTTTGTAGGGACTTG+TGG + Chr4:25661538-25661557 None:intergenic 35.0%
!!! ACCCATGGTTTTAACTTTAC+AGG + Chr4:25660283-25660302 None:intergenic 35.0%
ACAATGCCTCTCATTGGATT+TGG - Chr4:25659308-25659327 MsG0480019714.01.T01:CDS 40.0%
AGAAAGCTGAGAAAGACATG+TGG - Chr4:25660885-25660904 MsG0480019714.01.T01:CDS 40.0%
ATCTTGAGACAACATGGGTA+GGG - Chr4:25660344-25660363 MsG0480019714.01.T01:intron 40.0%
CAAAAACAGGAATCTGGACA+TGG - Chr4:25660228-25660247 MsG0480019714.01.T01:intron 40.0%
GGCCTGTAAAGTTAAAACCA+TGG - Chr4:25660278-25660297 MsG0480019714.01.T01:intron 40.0%
GTCTTGAAATGGGGTTATGT+AGG - Chr4:25660377-25660396 MsG0480019714.01.T01:CDS 40.0%
GTTTGTTTCAATGCAGCAAC+AGG + Chr4:25660035-25660054 None:intergenic 40.0%
TAAGTGCCAAATCCAATGAG+AGG + Chr4:25659317-25659336 None:intergenic 40.0%
TACACCTCCAGTTGTTAATC+AGG - Chr4:25660459-25660478 MsG0480019714.01.T01:CDS 40.0%
TCTCTAGCAACAAGATTGAG+TGG - Chr4:25660419-25660438 MsG0480019714.01.T01:CDS 40.0%
TCTTGACATCCAAATTGTGG+GGG - Chr4:25659994-25660013 MsG0480019714.01.T01:intron 40.0%
TGGATATGTACCTTGTGCAT+TGG - Chr4:25660257-25660276 MsG0480019714.01.T01:intron 40.0%
TTTATGGCATTGCCTAATGC+TGG + Chr4:25659933-25659952 None:intergenic 40.0%
! TTTTGTAGGGACTTGTGGTT+TGG + Chr4:25661533-25661552 None:intergenic 40.0%
ACTTTACAGGCCAATGCACA+AGG + Chr4:25660270-25660289 None:intergenic 45.0%
AGGTGGAAATGCATCCTATG+TGG - Chr4:25660856-25660875 MsG0480019714.01.T01:CDS 45.0%
ATGGGGTTATGTAGGTCCAT+TGG - Chr4:25660385-25660404 MsG0480019714.01.T01:CDS 45.0%
GATCTTGAGACAACATGGGT+AGG - Chr4:25660343-25660362 MsG0480019714.01.T01:intron 45.0%
GGAACAGGATAGTTAACCCA+TGG + Chr4:25660298-25660317 None:intergenic 45.0%
GGCATTACAATGCCTCTCAT+TGG - Chr4:25659302-25659321 MsG0480019714.01.T01:CDS 45.0%
TCAATGCAGCAACAGGATCA+TGG + Chr4:25660028-25660047 None:intergenic 45.0%
TGGAAATGCATCCTATGTGG+AGG - Chr4:25660859-25660878 MsG0480019714.01.T01:CDS 45.0%
! TGGGGTTGCTTTGTGTTTGA+AGG + Chr4:25661012-25661031 None:intergenic 45.0%
!! TTAGTACCTCAAGGGCATTG+TGG + Chr4:25659379-25659398 None:intergenic 45.0%
!! TTAAATTAATATAGTAAAAT+AGG - Chr4:25659766-25659785 MsG0480019714.01.T01:intron 5.0%
AGCTTTCTCTGCCTCCACAT+AGG + Chr4:25660873-25660892 None:intergenic 50.0%
GTAAGTGCCTACTCACCACT+TGG - Chr4:25660924-25660943 MsG0480019714.01.T01:CDS 50.0%
GTGATCACTCCCCCACAATT+TGG + Chr4:25660006-25660025 None:intergenic 50.0%
TGAGACAACATGGGTAGGGA+TGG - Chr4:25660348-25660367 MsG0480019714.01.T01:intron 50.0%
!! TATGGCTCTGAGCCAGCATT+AGG - Chr4:25659918-25659937 MsG0480019714.01.T01:CDS 50.0%
ACACAAAGCAACCCCAGCAC+AGG - Chr4:25661016-25661035 MsG0480019714.01.T01:intron 55.0%
AGTGCCTACTCACCACTTGG+TGG - Chr4:25660927-25660946 MsG0480019714.01.T01:CDS 55.0%
GTTGATCCCCATGCATTCCC+TGG + Chr4:25660954-25660973 None:intergenic 55.0%
TGCAGTCCACAATGCCCTTG+AGG - Chr4:25659370-25659389 MsG0480019714.01.T01:CDS 55.0%
TGGTCCACCAAGTGGTGAGT+AGG + Chr4:25660934-25660953 None:intergenic 55.0%
! ACTCACCACTTGGTGGACCA+GGG - Chr4:25660934-25660953 MsG0480019714.01.T01:CDS 55.0%
! TACTCACCACTTGGTGGACC+AGG - Chr4:25660933-25660952 MsG0480019714.01.T01:CDS 55.0%
! TGGTGGACCAGGGAATGCAT+GGG - Chr4:25660944-25660963 MsG0480019714.01.T01:CDS 55.0%
! TTGGTGGACCAGGGAATGCA+TGG - Chr4:25660943-25660962 MsG0480019714.01.T01:CDS 55.0%
GCATTCCCTGGTCCACCAAG+TGG + Chr4:25660942-25660961 None:intergenic 60.0%
GGTGGACCAGGGAATGCATG+GGG - Chr4:25660945-25660964 MsG0480019714.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 25659272 25661584 25659272 ID=MsG0480019714.01;Name=MsG0480019714.01
Chr4 mRNA 25659272 25661584 25659272 ID=MsG0480019714.01.T01;Parent=MsG0480019714.01;Name=MsG0480019714.01.T01;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|5|0|315
Chr4 exon 25661465 25661584 25661465 ID=MsG0480019714.01.T01:exon:25066;Parent=MsG0480019714.01.T01
Chr4 exon 25660796 25660971 25660796 ID=MsG0480019714.01.T01:exon:25065;Parent=MsG0480019714.01.T01
Chr4 exon 25660376 25660619 25660376 ID=MsG0480019714.01.T01:exon:25064;Parent=MsG0480019714.01.T01
Chr4 exon 25659819 25659998 25659819 ID=MsG0480019714.01.T01:exon:25063;Parent=MsG0480019714.01.T01
Chr4 exon 25659272 25659499 25659272 ID=MsG0480019714.01.T01:exon:25062;Parent=MsG0480019714.01.T01
Chr4 CDS 25661465 25661584 25661465 ID=MsG0480019714.01.T01:cds;Parent=MsG0480019714.01.T01
Chr4 CDS 25660796 25660971 25660796 ID=MsG0480019714.01.T01:cds;Parent=MsG0480019714.01.T01
Chr4 CDS 25660376 25660619 25660376 ID=MsG0480019714.01.T01:cds;Parent=MsG0480019714.01.T01
Chr4 CDS 25659819 25659998 25659819 ID=MsG0480019714.01.T01:cds;Parent=MsG0480019714.01.T01
Chr4 CDS 25659272 25659499 25659272 ID=MsG0480019714.01.T01:cds;Parent=MsG0480019714.01.T01
Gene Sequence

>MsG0480019714.01.T01

ATGAGGTGCAATCATGTGCGTTTGAATTGTGGCATTACAATGCCTCTCATTGGATTTGGCACTTATTCCTACCCAAATGATAAGAAGACAACAGAAAATGCAGTCCACAATGCCCTTGAGGTGGGTTATAGACATTTTGATACAGCAAAAATTTATGGCTCTGAGCCAGCATTAGGCAATGCCATAAATAAGGCAATCTATATGGGAGAAGTAGAAAGGGAAGATATTTTCTTGACATCCAAATTGTGGGGGAGTGATCACCATGATCCTGTTGCTGCATTGAAACAAACTCTAAAGAATCTGGACATGGAATATTTGGATATGTACCTTGTGCATTGGCCTGTAAAGTTAAAACCATGGGTTAACTATCCTGTTCCTAATGAAGATGACTTTGAAAATTTGGATCTTGAGACAACATGGGTAGGGATGGAAAAATGTCTTGAAATGGGGTTATGTAGGTCCATTGGAGTTAGTAATTTCTCTAGCAACAAGATTGAGTGGCTCTTAGATTTTGCTAATACACCTCCAGTTGTTAATCAGGTGGAAATGCATCCTATGTGGAGGCAGAGAAAGCTGAGAAAGACATGTGGAGAGCACAAGATACATGTAAGTGCCTACTCACCACTTGGTGGACCAGGGAATGCATGGGGATCAACAGCTGTTGTTAATCATCCAATTATACAATCAATTGCCTTCAAACACAAAGCAACCCCAGCACAGGTTGCATTGAAATGGGGATTATCAAAGGGCTCAAGTGTGATTGTGAAAAGTTTCAATGATGAAAGAATGGAGGAAAATATGGGATCATTTGATCTAAACTTGGATGATGAAGATATATTTGAGATTGAAAAATTGGAAGAAATGAAGATTATGAGAGGGGAATTTCATGTTAACCAAACCACAAGTCCCTACAAAACAATTGAAGAACTTTGGGATGATGAAATTTGA
Protein sequence

>MsG0480019714.01.T01

MRCNHVRLNCGITMPLIGFGTYSYPNDKKTTENAVHNALEVGYRHFDTAKIYGSEPALGNAINKAIYMGEVEREDIFLTSKLWGSDHHDPVAALKQTLKNLDMEYLDMYLVHWPVKLKPWVNYPVPNEDDFENLDLETTWVGMEKCLEMGLCRSIGVSNFSSNKIEWLLDFANTPPVVNQVEMHPMWRQRKLRKTCGEHKIHVSAYSPLGGPGNAWGSTAVVNHPIIQSIAFKHKATPAQVALKWGLSKGSSVIVKSFNDERMEENMGSFDLNLDDEDIFEIEKLEEMKIMRGEFHVNQTTSPYKTIEELWDDEI*