Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035213.01.T01 | XP_039682635.1 | 82.238 | 563 | 86 | 3 | 1 | 550 | 9 | 570 | 0 | 917 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035213.01.T01 | MTR_6g077540 | 82.238 | 563 | 86 | 3 | 1 | 550 | 9 | 570 | 0.0 | 917 |
| MsG0680035213.01.T01 | MTR_6g065540 | 80.470 | 553 | 102 | 3 | 1 | 550 | 8 | 557 | 0.0 | 892 |
| MsG0680035213.01.T01 | MTR_6g079830 | 77.936 | 562 | 111 | 2 | 1 | 549 | 10 | 571 | 0.0 | 878 |
| MsG0680035213.01.T01 | MTR_6g079830 | 31.830 | 377 | 257 | 0 | 70 | 446 | 197 | 573 | 5.12e-72 | 243 |
| MsG0680035213.01.T01 | MTR_6g079830 | 32.370 | 346 | 234 | 0 | 70 | 415 | 232 | 577 | 4.32e-56 | 199 |
| MsG0680035213.01.T01 | MTR_6g079920 | 77.936 | 562 | 111 | 2 | 1 | 549 | 10 | 571 | 0.0 | 877 |
| MsG0680035213.01.T01 | MTR_6g079920 | 32.368 | 380 | 257 | 0 | 70 | 449 | 197 | 576 | 1.73e-72 | 242 |
| MsG0680035213.01.T01 | MTR_6g079310 | 80.200 | 500 | 96 | 1 | 54 | 550 | 2 | 501 | 0.0 | 822 |
| MsG0680035213.01.T01 | MTR_6g079310 | 27.457 | 346 | 219 | 1 | 30 | 375 | 188 | 501 | 2.85e-46 | 169 |
| MsG0680035213.01.T01 | MTR_6g079310 | 27.931 | 290 | 209 | 0 | 260 | 549 | 1 | 290 | 4.07e-36 | 141 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035213.01.T01 | AT1G12700 | 38.767 | 503 | 305 | 1 | 49 | 548 | 71 | 573 | 1.03e-131 | 397 |
| MsG0680035213.01.T01 | AT1G12700 | 30.000 | 350 | 245 | 0 | 201 | 550 | 86 | 435 | 1.21e-52 | 189 |
| MsG0680035213.01.T01 | AT1G12700 | 29.305 | 331 | 234 | 0 | 220 | 550 | 70 | 400 | 9.06e-51 | 184 |
| MsG0680035213.01.T01 | AT1G12700 | 38.767 | 503 | 305 | 1 | 49 | 548 | 87 | 589 | 1.17e-131 | 397 |
| MsG0680035213.01.T01 | AT1G12700 | 30.000 | 350 | 245 | 0 | 201 | 550 | 102 | 451 | 1.34e-52 | 189 |
| MsG0680035213.01.T01 | AT1G12700 | 29.305 | 331 | 234 | 0 | 220 | 550 | 86 | 416 | 9.96e-51 | 184 |
| MsG0680035213.01.T01 | AT1G12700 | 38.767 | 503 | 305 | 1 | 49 | 548 | 87 | 589 | 1.17e-131 | 397 |
| MsG0680035213.01.T01 | AT1G12700 | 30.000 | 350 | 245 | 0 | 201 | 550 | 102 | 451 | 1.34e-52 | 189 |
| MsG0680035213.01.T01 | AT1G12700 | 29.305 | 331 | 234 | 0 | 220 | 550 | 86 | 416 | 9.96e-51 | 184 |
| MsG0680035213.01.T01 | AT3G22470 | 38.433 | 536 | 292 | 2 | 47 | 544 | 51 | 586 | 1.46e-128 | 389 |
Find 96 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATTCGAAGGCGGGTGGAG+TGG | 0.530646 | 6:-100684215 | None:intergenic |
| AACCTTCACTACACTCATCA+AGG | 0.491635 | 6:+100684432 | MsG0680035213.01.T01:CDS |
| AACTCGTCGGAGCAATTGAA+GGG | 0.602311 | 6:-100684590 | None:intergenic |
| AAGAACAGCGTGGTAATGTT+TGG | 0.363925 | 6:-100684257 | None:intergenic |
| AAGGGAACCTACAAATTCGA+AGG | 0.608724 | 6:-100684227 | None:intergenic |
| AATTCGAAGGCGGGTGGAGT+GGG | 0.530549 | 6:-100684214 | None:intergenic |
| AATTGAACCAAGTTAGTTAT+GGG | 0.363138 | 6:+100684524 | MsG0680035213.01.T01:CDS |
| AATTGCTCCGACGAGTTGAT+GGG | 0.512324 | 6:+100684596 | MsG0680035213.01.T01:CDS |
| ACAAAACCCATGGATCATAA+CGG | 0.595085 | 6:-100685490 | None:intergenic |
| ACATACACTATTCTTATTGA+TGG | 0.276400 | 6:+100685378 | MsG0680035213.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTTACAATTCCCTTATTGA+TGG | + | Chr6:100685168-100685187 | MsG0680035213.01.T01:CDS | 30.0% | |
| ACTTACAGTGCTTTAATTAG+TGG | + | Chr6:100684748-100684767 | MsG0680035213.01.T01:CDS | 30.0% | |
| ATGTTGCAATTGACTAAGTT+AGG | - | Chr6:100684319-100684338 | None:intergenic | 30.0% | |
| CAATTGAACCAAGTTAGTTA+TGG | + | Chr6:100684523-100684542 | MsG0680035213.01.T01:CDS | 30.0% | |
| CAGAAAAATTATTCCTGATG+TGG | + | Chr6:100685143-100685162 | MsG0680035213.01.T01:CDS | 30.0% | |
| CATCAATTCTTTCTGTCAAT+TGG | + | Chr6:100684342-100684361 | MsG0680035213.01.T01:CDS | 30.0% | |
| CATCTTTATCAAACAGAGAA+CGG | - | Chr6:100685608-100685627 | None:intergenic | 30.0% | |
| CATTTCTTCAAAGAGATTCA+TGG | - | Chr6:100685118-100685137 | None:intergenic | 30.0% | |
| CCTAGTTAAAGAAGTGAATA+AGG | + | Chr6:100684987-100685006 | MsG0680035213.01.T01:CDS | 30.0% | |
| CCTTATTCACTTCTTTAACT+AGG | - | Chr6:100684990-100685009 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 100684031 | 100685683 | 100684031 | ID=MsG0680035213.01;Name=MsG0680035213.01 |
| Chr6 | mRNA | 100684031 | 100685683 | 100684031 | ID=MsG0680035213.01.T01;Parent=MsG0680035213.01;Name=MsG0680035213.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|550 |
| Chr6 | exon | 100684031 | 100685683 | 100684031 | ID=MsG0680035213.01.T01:exon:20637;Parent=MsG0680035213.01.T01 |
| Chr6 | CDS | 100684031 | 100685683 | 100684031 | ID=MsG0680035213.01.T01:cds;Parent=MsG0680035213.01.T01 |
| Gene Sequence |
>MsG0680035213.01.T01 ATGTTGCTGAGGTACTGTGTTGTTCCTGTTTCTCTTTTTGTTCCAAAAAAGTTTCATTCTTTTCAATTTCTGAAAAACACCAAATTCAATTTCATTCCTTATTCTTCAACTTCATCCACATTCCATAATAACAATGATGTTGTTGATGCTGTTTCATCGTTCAATTGCTTGCTTCATCGAAATCCCACTCCACCCGCCTTCGAATTTGTAGGTTCCCTTGTCAAATCCAAACATTACCACGCTGTTCTTTCCCTTTTTCAACAAATGGAATTTATAGGAATTAAACCTAACTTAGTCAATTGCAACATTCTCATCAATTCTTTCTGTCAATTGGGTCTTATCTCTTTTGCTTTTTCTGTATTCACAAAGATTCTCAAAAATGGTTACGAGCCAGATACCATAACCTTCACTACACTCATCAAGGGTCTCTGTCTCAAAGGTGACATCCATCAAGCATTGCACTTTCATGACAAGCTGGTAGCGCTTGGATTTCAATTGAACCAAGTTAGTTATGGGACCTTGATTAATGGCTTGTGTAAAGTTAGTGAAACAAGAGCAGCCCTTCAATTGCTCCGACGAGTTGATGGGAAATTGGTTCGGCCTGATGTGGTAATGTACAGTACGATTATTGATAGTATGTGCAAAGTTAAACTTGTAAATGATGCTTTTGATTTATATTCTGAAATGGTTTCTAAGAGAATTTCTCCTGATGTTGTTACTTACAGTGCTTTAATTAGTGGCTTTTGCATTGTTGGTGAATTGAAAGATGCAATTGGTTTGTTCAATAAAATGATATTGGAAAACATCATCCCGGATGTGTATACCTTTAGTATATTGGTTGATGGATTTTGTAAGGAAGGAAGGGTGAAAGAAGCTAAAAATGTGTTCGCTATGATGATGAAACAAGATATTAAACCTAATGTTTTTACTTATAACTCTTTAATGGATGGATATTGCCTAGTTAAAGAAGTGAATAAGGCTAAGAGTATATTCAACACTATGGCTCAGGGGGGAGTGAATCCTGATATTCACAGTTATAGTATCTTGATTAATGGGTTTTGTAAGATTAAAATGATGGATGAAGCCATGAATCTCTTTGAAGAAATGCATTGCAGAAAAATTATTCCTGATGTGGTAACTTACAATTCCCTTATTGATGGTTTGTGTAAATCGGGGAAAATCTCTTATGCTTTGAAGCTAGTCAATGAGATGCATGATAGAGGTCAAGCACCTGATATGATTACTTACAATTCTATATTGGATGCTTTGTGCAAAAACCATCAGGTTGACAGGGCGATTGCATTATTAACAAAATTGAAAGACCAGGGTATTCGACCAAATATGTACACATACACTATTCTTATTGATGGATTGTGTAAAGGTGGAAGACTAGAGGATGCACAAAATGTTTTTGAGGATCTTTTAGTCAAAGGCTATAATATAACTGTCAACACATATACCGTTATGATCCATGGGTTTTGTAACAAGGGCTTGTTTGATGAAGCATTGGCTTTGTTGTCAAAAATGAAAGACAATAGTTGCATTCCAGACGCCGTTACTTATGAAATAATCATCCGTTCTCTGTTTGATAAAGATGAAAATGATAAGGCGGAGAAACTTCTTCGTGAAATGATTACGAGAGGCCTATTGTAA |
| Protein sequence |
>MsG0680035213.01.T01 MLLRYCVVPVSLFVPKKFHSFQFLKNTKFNFIPYSSTSSTFHNNNDVVDAVSSFNCLLHRNPTPPAFEFVGSLVKSKHYHAVLSLFQQMEFIGIKPNLVNCNILINSFCQLGLISFAFSVFTKILKNGYEPDTITFTTLIKGLCLKGDIHQALHFHDKLVALGFQLNQVSYGTLINGLCKVSETRAALQLLRRVDGKLVRPDVVMYSTIIDSMCKVKLVNDAFDLYSEMVSKRISPDVVTYSALISGFCIVGELKDAIGLFNKMILENIIPDVYTFSILVDGFCKEGRVKEAKNVFAMMMKQDIKPNVFTYNSLMDGYCLVKEVNKAKSIFNTMAQGGVNPDIHSYSILINGFCKIKMMDEAMNLFEEMHCRKIIPDVVTYNSLIDGLCKSGKISYALKLVNEMHDRGQAPDMITYNSILDALCKNHQVDRAIALLTKLKDQGIRPNMYTYTILIDGLCKGGRLEDAQNVFEDLLVKGYNITVNTYTVMIHGFCNKGLFDEALALLSKMKDNSCIPDAVTYEIIIRSLFDKDENDKAEKLLREMITRGLL* |
