Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003762.01.T01 | XP_013468395.1 | 97.625 | 421 | 9 | 1 | 1 | 420 | 1 | 421 | 0 | 841 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003762.01.T01 | Q9SVU0 | 72.17 | 424 | 113 | 2 | 1 | 419 | 1 | 424 | 0 | 648 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003762.01.T01 | A0A072VKW7 | 97.625 | 421 | 9 | 1 | 1 | 420 | 1 | 421 | 0.0 | 841 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003762.01.T01 | MTR_1g069275 | 97.625 | 421 | 9 | 1 | 1 | 420 | 1 | 421 | 0.0 | 841 |
| MsG0180003762.01.T01 | MTR_7g099330 | 78.622 | 421 | 84 | 3 | 1 | 420 | 1 | 416 | 0.0 | 692 |
| MsG0180003762.01.T01 | MTR_1g046230 | 72.530 | 415 | 110 | 1 | 10 | 420 | 9 | 423 | 0.0 | 626 |
| MsG0180003762.01.T01 | MTR_7g099160 | 77.508 | 329 | 69 | 2 | 93 | 420 | 213 | 537 | 0.0 | 529 |
| MsG0180003762.01.T01 | MTR_1g008380 | 63.753 | 389 | 139 | 2 | 18 | 404 | 35 | 423 | 0.0 | 522 |
| MsG0180003762.01.T01 | MTR_3g109520 | 60.298 | 403 | 157 | 2 | 4 | 403 | 18 | 420 | 0.0 | 512 |
| MsG0180003762.01.T01 | MTR_6g086870 | 61.026 | 390 | 145 | 4 | 22 | 405 | 17 | 405 | 1.27e-178 | 504 |
| MsG0180003762.01.T01 | MTR_1g011630 | 59.162 | 382 | 154 | 1 | 18 | 399 | 9 | 388 | 2.72e-178 | 503 |
| MsG0180003762.01.T01 | MTR_7g107710 | 47.368 | 380 | 191 | 4 | 26 | 401 | 7 | 381 | 4.31e-123 | 362 |
| MsG0180003762.01.T01 | MTR_8g432640 | 47.733 | 375 | 187 | 4 | 26 | 396 | 7 | 376 | 1.74e-120 | 355 |
| MsG0180003762.01.T01 | MTR_5g033260 | 46.842 | 380 | 187 | 5 | 26 | 398 | 7 | 378 | 6.28e-120 | 354 |
| MsG0180003762.01.T01 | MTR_7g107690 | 44.327 | 379 | 202 | 4 | 26 | 400 | 9 | 382 | 6.35e-120 | 354 |
| MsG0180003762.01.T01 | MTR_3g088925 | 44.267 | 375 | 198 | 5 | 26 | 395 | 8 | 376 | 7.60e-117 | 347 |
| MsG0180003762.01.T01 | MTR_3g088945 | 44.000 | 375 | 199 | 5 | 26 | 395 | 8 | 376 | 6.82e-116 | 344 |
| MsG0180003762.01.T01 | MTR_3g088955 | 43.467 | 375 | 201 | 5 | 26 | 395 | 8 | 376 | 6.65e-114 | 339 |
| MsG0180003762.01.T01 | MTR_4g051642 | 45.067 | 375 | 194 | 6 | 26 | 395 | 8 | 375 | 1.26e-113 | 338 |
| MsG0180003762.01.T01 | MTR_3g096030 | 38.068 | 176 | 83 | 2 | 26 | 199 | 7 | 158 | 8.82e-37 | 132 |
| MsG0180003762.01.T01 | MTR_2g450510 | 28.632 | 234 | 129 | 8 | 27 | 223 | 7 | 239 | 1.38e-14 | 75.9 |
| MsG0180003762.01.T01 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 1.89e-14 | 73.6 |
| MsG0180003762.01.T01 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 2.57e-14 | 73.9 |
| MsG0180003762.01.T01 | MTR_3g437580 | 24.017 | 229 | 139 | 5 | 27 | 220 | 15 | 243 | 3.76e-14 | 73.9 |
| MsG0180003762.01.T01 | MTR_8g037910 | 22.967 | 209 | 131 | 3 | 27 | 208 | 6 | 211 | 4.15e-14 | 73.9 |
| MsG0180003762.01.T01 | MTR_3g437580 | 24.017 | 229 | 139 | 5 | 27 | 220 | 15 | 243 | 6.64e-14 | 73.6 |
| MsG0180003762.01.T01 | MTR_2g044140 | 26.757 | 370 | 207 | 14 | 27 | 336 | 16 | 381 | 3.88e-12 | 68.2 |
| MsG0180003762.01.T01 | MTR_2g043850 | 25.806 | 372 | 208 | 16 | 27 | 336 | 16 | 381 | 2.76e-11 | 65.9 |
| MsG0180003762.01.T01 | MTR_2g020630 | 25.417 | 240 | 138 | 9 | 27 | 227 | 16 | 253 | 3.75e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003762.01.T01 | AT4G28720 | 72.170 | 424 | 113 | 2 | 1 | 419 | 1 | 424 | 0.0 | 648 |
| MsG0180003762.01.T01 | AT1G04180 | 71.122 | 419 | 120 | 1 | 1 | 418 | 1 | 419 | 0.0 | 624 |
| MsG0180003762.01.T01 | AT1G04610 | 69.690 | 419 | 122 | 1 | 7 | 420 | 19 | 437 | 0.0 | 615 |
| MsG0180003762.01.T01 | AT5G43890 | 69.194 | 422 | 126 | 2 | 1 | 418 | 1 | 422 | 0.0 | 613 |
| MsG0180003762.01.T01 | AT2G33230 | 69.565 | 414 | 120 | 2 | 12 | 420 | 19 | 431 | 0.0 | 584 |
| MsG0180003762.01.T01 | AT5G25620 | 60.247 | 405 | 157 | 3 | 6 | 406 | 12 | 416 | 3.49e-179 | 506 |
| MsG0180003762.01.T01 | AT5G25620 | 60.500 | 400 | 154 | 3 | 6 | 401 | 12 | 411 | 3.41e-178 | 504 |
| MsG0180003762.01.T01 | AT4G32540 | 55.257 | 409 | 176 | 4 | 10 | 417 | 7 | 409 | 1.79e-172 | 489 |
| MsG0180003762.01.T01 | AT5G11320 | 59.843 | 381 | 149 | 1 | 20 | 400 | 12 | 388 | 2.10e-172 | 489 |
| MsG0180003762.01.T01 | AT4G13260 | 60.789 | 380 | 146 | 2 | 18 | 394 | 21 | 400 | 2.19e-171 | 486 |
| MsG0180003762.01.T01 | AT4G32540 | 55.231 | 411 | 175 | 5 | 10 | 417 | 7 | 411 | 7.14e-171 | 485 |
| MsG0180003762.01.T01 | AT5G11320 | 59.202 | 326 | 129 | 1 | 20 | 345 | 12 | 333 | 1.29e-142 | 411 |
| MsG0180003762.01.T01 | AT4G13260 | 61.379 | 290 | 110 | 1 | 18 | 305 | 21 | 310 | 1.02e-131 | 381 |
| MsG0180003762.01.T01 | AT1G48910 | 47.215 | 377 | 188 | 5 | 26 | 397 | 6 | 376 | 9.99e-126 | 369 |
| MsG0180003762.01.T01 | AT1G21430 | 48.396 | 374 | 181 | 5 | 26 | 394 | 10 | 376 | 3.16e-123 | 363 |
| MsG0180003762.01.T01 | AT4G13260 | 58.131 | 289 | 118 | 2 | 109 | 394 | 9 | 297 | 1.68e-114 | 338 |
| MsG0180003762.01.T01 | AT1G62560 | 27.230 | 213 | 122 | 6 | 27 | 208 | 15 | 225 | 2.66e-19 | 89.7 |
| MsG0180003762.01.T01 | AT1G62560 | 27.230 | 213 | 122 | 6 | 27 | 208 | 15 | 225 | 5.29e-19 | 89.4 |
| MsG0180003762.01.T01 | AT5G45180 | 26.136 | 352 | 192 | 14 | 27 | 336 | 15 | 340 | 2.02e-17 | 84.3 |
| MsG0180003762.01.T01 | AT1G65860 | 25.561 | 223 | 138 | 4 | 27 | 221 | 15 | 237 | 3.15e-16 | 80.9 |
| MsG0180003762.01.T01 | AT1G12160 | 26.636 | 214 | 124 | 6 | 27 | 209 | 14 | 225 | 1.08e-15 | 79.3 |
| MsG0180003762.01.T01 | AT1G62620 | 25.926 | 243 | 138 | 8 | 27 | 234 | 15 | 250 | 1.88e-15 | 78.2 |
| MsG0180003762.01.T01 | AT5G07800 | 26.147 | 218 | 125 | 5 | 27 | 208 | 18 | 235 | 1.88e-15 | 78.6 |
| MsG0180003762.01.T01 | AT1G63370 | 25.000 | 240 | 144 | 7 | 27 | 234 | 15 | 250 | 3.11e-15 | 77.0 |
| MsG0180003762.01.T01 | AT1G62620 | 25.417 | 240 | 143 | 7 | 27 | 234 | 15 | 250 | 3.92e-15 | 77.4 |
| MsG0180003762.01.T01 | AT1G63370 | 25.000 | 240 | 144 | 7 | 27 | 234 | 15 | 250 | 7.44e-15 | 76.6 |
| MsG0180003762.01.T01 | AT5G61290 | 26.267 | 217 | 126 | 5 | 27 | 209 | 18 | 234 | 1.54e-14 | 75.5 |
| MsG0180003762.01.T01 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.08e-14 | 74.7 |
| MsG0180003762.01.T01 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.39e-14 | 74.7 |
| MsG0180003762.01.T01 | AT1G62540 | 24.880 | 209 | 129 | 3 | 41 | 221 | 29 | 237 | 3.67e-14 | 74.3 |
| MsG0180003762.01.T01 | AT1G12200 | 25.743 | 202 | 117 | 4 | 41 | 209 | 30 | 231 | 5.48e-14 | 73.6 |
| MsG0180003762.01.T01 | AT1G12200 | 25.743 | 202 | 117 | 4 | 41 | 209 | 30 | 231 | 1.44e-13 | 72.8 |
| MsG0180003762.01.T01 | AT1G12130 | 23.451 | 226 | 142 | 5 | 41 | 235 | 29 | 254 | 5.33e-13 | 70.9 |
| MsG0180003762.01.T01 | AT1G62570 | 22.967 | 209 | 133 | 3 | 41 | 221 | 29 | 237 | 1.70e-12 | 69.3 |
| MsG0180003762.01.T01 | AT1G12140 | 22.857 | 210 | 133 | 3 | 41 | 221 | 29 | 238 | 2.15e-12 | 68.9 |
| MsG0180003762.01.T01 | AT1G12140 | 22.857 | 210 | 133 | 3 | 41 | 221 | 29 | 238 | 2.15e-12 | 68.9 |
| MsG0180003762.01.T01 | AT1G62600 | 24.464 | 233 | 126 | 7 | 34 | 219 | 22 | 251 | 9.45e-11 | 63.5 |
Find 91 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTTCTTTGTTGGATATTC+AGG | 0.151113 | 1:+67807373 | None:intergenic |
| GTTGTTGGTTGTGGTAATTC+TGG | 0.205623 | 1:-67807064 | MsG0180003762.01.T01:CDS |
| GGTAGTTGACAGAATTGTTT+AGG | 0.250500 | 1:+67807421 | None:intergenic |
| CTCAAGATATTGCTCAAGTT+TGG | 0.269888 | 1:-67805837 | MsG0180003762.01.T01:CDS |
| ATCGTAGCAATGTCCCTTCT+TGG | 0.282135 | 1:-67806341 | MsG0180003762.01.T01:CDS |
| TAGAAAAGTATGGTCTTAAA+AGG | 0.282601 | 1:-67806554 | MsG0180003762.01.T01:CDS |
| CAATTTGTGCAATTTAGAAT+TGG | 0.285416 | 1:+67805755 | None:intergenic |
| GCTAAATATGATGAGACTAA+TGG | 0.295504 | 1:-67807277 | MsG0180003762.01.T01:CDS |
| GGTCCTGCTCCTACTATTAT+AGG | 0.301581 | 1:+67807568 | None:intergenic |
| CATGCTTGTGATTATAAATC+AGG | 0.314519 | 1:-67807115 | MsG0180003762.01.T01:CDS |
| AAAGGGTAAGACCCCTGTTT+TGG | 0.316214 | 1:-67806499 | MsG0180003762.01.T01:CDS |
| TTACTCAACCCTTCAATATC+AGG | 0.327104 | 1:+67807157 | None:intergenic |
| GGCTGTGTTGAACTTGTTAA+TGG | 0.328799 | 1:-67806405 | MsG0180003762.01.T01:CDS |
| TTTATGAACTGTTTCTTTGT+TGG | 0.340000 | 1:+67807364 | None:intergenic |
| CAGTTCCTTTCCAACCATTA+GGG | 0.343203 | 1:+67805929 | None:intergenic |
| TAGTCTCACATCGTTGCATT+TGG | 0.343936 | 1:-67807600 | MsG0180003762.01.T01:CDS |
| AAGGCCATTGGAGGGTCCTT+TGG | 0.345490 | 1:-67806535 | MsG0180003762.01.T01:CDS |
| ACAGTTCCTTTCCAACCATT+AGG | 0.345992 | 1:+67805928 | None:intergenic |
| CAATGTCCCTTCTTGGCTTA+AGG | 0.348481 | 1:-67806334 | MsG0180003762.01.T01:intron |
| TGACTTTGGAAATCCATTCT+TGG | 0.361539 | 1:+67805957 | None:intergenic |
| GTAGTTGACAGAATTGTTTA+GGG | 0.362984 | 1:+67807422 | None:intergenic |
| CCAAAGTCACCATTCCCTAA+TGG | 0.366181 | 1:-67805943 | MsG0180003762.01.T01:CDS |
| TTAGTAAACCTCTTAATTCC+AGG | 0.368388 | 1:+67806429 | None:intergenic |
| TCTTGTGGGAATGGAAAGTT+AGG | 0.369111 | 1:+67807400 | None:intergenic |
| CTGTTGGACTTTATGCTGTT+GGG | 0.378354 | 1:-67805909 | MsG0180003762.01.T01:CDS |
| CATCGTTGCATTTGGGTAAA+CGG | 0.385627 | 1:-67807592 | MsG0180003762.01.T01:CDS |
| AATTTGTGCAATTTAGAATT+GGG | 0.388229 | 1:+67805756 | None:intergenic |
| AAGGTGCCAATATCCAAAAC+AGG | 0.397457 | 1:+67806486 | None:intergenic |
| TTCTATTGTTCTTCGTAGCT+CGG | 0.402239 | 1:-67806999 | MsG0180003762.01.T01:intron |
| ATACTCAACTTCATTTCCAT+TGG | 0.414194 | 1:+67807231 | None:intergenic |
| AGTCTCACATCGTTGCATTT+GGG | 0.415427 | 1:-67807599 | MsG0180003762.01.T01:CDS |
| TTTGGTAATATAGAAAAGTA+TGG | 0.417326 | 1:-67806564 | MsG0180003762.01.T01:CDS |
| CTAAATATGATGAGACTAAT+GGG | 0.418507 | 1:-67807276 | MsG0180003762.01.T01:CDS |
| TGATGAAATGGTTACCCGTT+TGG | 0.423835 | 1:-67806626 | MsG0180003762.01.T01:CDS |
| AGGTCGCTTGTGGTTGCTAC+TGG | 0.431631 | 1:-67807202 | MsG0180003762.01.T01:CDS |
| GATGCAGTTGTTCTTGCTAC+TGG | 0.439635 | 1:-67806366 | MsG0180003762.01.T01:CDS |
| CCATTAGGGAATGGTGACTT+TGG | 0.441458 | 1:+67805943 | None:intergenic |
| ACAACAAGGAGTACCCTTCA+TGG | 0.462402 | 1:-67807515 | MsG0180003762.01.T01:CDS |
| TCAGGAAATTCTTGTGGGAA+TGG | 0.465607 | 1:+67807391 | None:intergenic |
| ATGAAGTTGAGTATATTTGT+AGG | 0.466018 | 1:-67807222 | MsG0180003762.01.T01:CDS |
| GATATTCAGGAAATTCTTGT+GGG | 0.470627 | 1:+67807386 | None:intergenic |
| ACTGTTGGACTTTATGCTGT+TGG | 0.470697 | 1:-67805910 | MsG0180003762.01.T01:CDS |
| ATAGTAGGAGCAGGACCATC+AGG | 0.478379 | 1:-67807562 | MsG0180003762.01.T01:CDS |
| TGGCAGTTATGATGATGAAA+TGG | 0.484448 | 1:-67806638 | MsG0180003762.01.T01:CDS |
| TTTCTCACCTTAAGCCAAGA+AGG | 0.485907 | 1:+67806327 | None:intergenic |
| TTTACAAAGAGAGGGCTCTC+TGG | 0.487445 | 1:-67805886 | MsG0180003762.01.T01:CDS |
| AATCTTGTCAACTATCCAAA+CGG | 0.487573 | 1:+67806611 | None:intergenic |
| TGGGAAATCAACATTTGGAT+TGG | 0.487762 | 1:-67806658 | MsG0180003762.01.T01:CDS |
| GGAATTAAGAGGTTTACTAA+TGG | 0.490761 | 1:-67806426 | MsG0180003762.01.T01:CDS |
| ATCTTGTCAACTATCCAAAC+GGG | 0.505639 | 1:+67806612 | None:intergenic |
| GAGCTACGAAGAACAATAGA+AGG | 0.506431 | 1:+67807001 | None:intergenic |
| TTGGAGCTTAAGAACACAAA+GGG | 0.508263 | 1:-67806516 | MsG0180003762.01.T01:CDS |
| GTAAACGGTCCTATAATAGT+AGG | 0.514321 | 1:-67807577 | MsG0180003762.01.T01:CDS |
| TGATCTTTCAAGCACCATGA+AGG | 0.516098 | 1:+67807501 | None:intergenic |
| AAGTTGTGCCTGGAATTAAG+AGG | 0.518103 | 1:-67806437 | MsG0180003762.01.T01:CDS |
| GGATATTCAGGAAATTCTTG+TGG | 0.520358 | 1:+67807385 | None:intergenic |
| CCTTTCCAACCATTAGGGAA+TGG | 0.520534 | 1:+67805934 | None:intergenic |
| ACCTTGGAGAAAATTAGATC+TGG | 0.532203 | 1:-67806468 | MsG0180003762.01.T01:CDS |
| GTATGGTCTTAAAAGGCCAT+TGG | 0.535113 | 1:-67806547 | MsG0180003762.01.T01:CDS |
| TTCTCACCTTAAGCCAAGAA+GGG | 0.539788 | 1:+67806328 | None:intergenic |
| GTTGACAGAATTGTTTAGGG+AGG | 0.539880 | 1:+67807425 | None:intergenic |
| GCTGTTGGGTTTACAAAGAG+AGG | 0.540106 | 1:-67805895 | MsG0180003762.01.T01:CDS |
| TTTGTGTTCTTAAGCTCCAA+AGG | 0.541522 | 1:+67806519 | None:intergenic |
| TGTGATTATAAATCAGGTGA+AGG | 0.541872 | 1:-67807109 | MsG0180003762.01.T01:CDS |
| TGGTCTTAAAAGGCCATTGG+AGG | 0.553703 | 1:-67806544 | MsG0180003762.01.T01:CDS |
| TGTGTTGTGCCTGATATTGA+AGG | 0.554440 | 1:-67807166 | MsG0180003762.01.T01:CDS |
| GCTGCTGTTGCTAGACCTGA+TGG | 0.555064 | 1:+67807547 | None:intergenic |
| AATGGTTGGAAAGGAACTGT+TGG | 0.555476 | 1:-67805925 | MsG0180003762.01.T01:CDS |
| AGTCACCATTCCCTAATGGT+TGG | 0.557817 | 1:-67805939 | MsG0180003762.01.T01:CDS |
| GAACACACTCGTTGAATCGT+GGG | 0.559010 | 1:+67807305 | None:intergenic |
| CCATTCCCTAATGGTTGGAA+AGG | 0.559102 | 1:-67805934 | MsG0180003762.01.T01:CDS |
| ATGATGAGACTAATGGGTTG+TGG | 0.559573 | 1:-67807270 | MsG0180003762.01.T01:CDS |
| GGTCACATAAAAGTTGTGCC+TGG | 0.561332 | 1:-67806447 | MsG0180003762.01.T01:CDS |
| TGGTTGTGGTAATTCTGGAA+TGG | 0.564985 | 1:-67807059 | MsG0180003762.01.T01:CDS |
| CTGTTGGGTTTACAAAGAGA+GGG | 0.568492 | 1:-67805894 | MsG0180003762.01.T01:CDS |
| TGAACACACTCGTTGAATCG+TGG | 0.570661 | 1:+67807304 | None:intergenic |
| GATCTTTCAAGCACCATGAA+GGG | 0.575566 | 1:+67807502 | None:intergenic |
| TATCTTCTTCATTATCGACT+AGG | 0.583142 | 1:+67807623 | None:intergenic |
| GATTTCGAAGTGTTTCGCGT+AGG | 0.586522 | 1:+67807330 | None:intergenic |
| GAAGGGTTGAGTAAATTCAA+AGG | 0.589620 | 1:-67807148 | MsG0180003762.01.T01:CDS |
| AGCTCCAAAGGACCCTCCAA+TGG | 0.594886 | 1:+67806531 | None:intergenic |
| GGTCCTATAATAGTAGGAGC+AGG | 0.599245 | 1:-67807571 | MsG0180003762.01.T01:CDS |
| AGAGTTGAGACTGTTTCCAA+TGG | 0.605340 | 1:-67807247 | MsG0180003762.01.T01:CDS |
| GTATATTTGTAGGTCGCTTG+TGG | 0.619170 | 1:-67807212 | MsG0180003762.01.T01:CDS |
| AGGTGCCAATATCCAAAACA+GGG | 0.629075 | 1:+67806487 | None:intergenic |
| GTGTTGTGCCTGATATTGAA+GGG | 0.636186 | 1:-67807165 | MsG0180003762.01.T01:CDS |
| GGTCTTAAAAGGCCATTGGA+GGG | 0.661835 | 1:-67806543 | MsG0180003762.01.T01:CDS |
| TTTGGAGCTTAAGAACACAA+AGG | 0.673099 | 1:-67806517 | MsG0180003762.01.T01:CDS |
| GCAGCATGTCTTAAACAACA+AGG | 0.727523 | 1:-67807529 | MsG0180003762.01.T01:CDS |
| GGTGCCAATATCCAAAACAG+GGG | 0.738091 | 1:+67806488 | None:intergenic |
| CAAATTGCATAGCTTCACTG+TGG | 0.767067 | 1:-67807480 | MsG0180003762.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTAAGAAAAATAATTTG+AGG | - | Chr1:67806643-67806662 | MsG0180003762.01.T01:CDS | 10.0% |
| !!! | TTTTTTTTTTGTACTAATTA+AGG | - | Chr1:67806678-67806697 | MsG0180003762.01.T01:CDS | 10.0% |
| !!! | GTTAATAACTAATCTTATTT+TGG | - | Chr1:67807395-67807414 | MsG0180003762.01.T01:CDS | 15.0% |
| !!! | AGCAATTTTAGAAACTAAAT+TGG | + | Chr1:67806534-67806553 | None:intergenic | 20.0% |
| !!! | AGTATTTTACTATTTTTGTG+TGG | - | Chr1:67807371-67807390 | MsG0180003762.01.T01:CDS | 20.0% |
| !!! | TTTGGTAATATAGAAAAGTA+TGG | - | Chr1:67806844-67806863 | MsG0180003762.01.T01:intron | 20.0% |
| ! | ATACTAATTCCATTTCCAAA+AGG | + | Chr1:67807356-67807375 | None:intergenic | 25.0% |
| ! | ATATGATAAGACCCAAAAAA+AGG | + | Chr1:67807252-67807271 | None:intergenic | 25.0% |
| ! | ATGAAGTTGAGTATATTTGT+AGG | - | Chr1:67806186-67806205 | MsG0180003762.01.T01:intron | 25.0% |
| ! | CTAAATATGATGAGACTAAT+GGG | - | Chr1:67806132-67806151 | MsG0180003762.01.T01:intron | 25.0% |
| ! | TACTAATTCCATTTCCAAAA+GGG | + | Chr1:67807355-67807374 | None:intergenic | 25.0% |
| !! | AATTAAGGTTGTTGTGTTTT+TGG | - | Chr1:67806693-67806712 | MsG0180003762.01.T01:intron | 25.0% |
| !! | TAGAAAAGTATGGTCTTAAA+AGG | - | Chr1:67806854-67806873 | MsG0180003762.01.T01:intron | 25.0% |
| !! | TTTATGAACTGTTTCTTTGT+TGG | + | Chr1:67806047-67806066 | None:intergenic | 25.0% |
| !!! | TGACAAGATTCTTTTGATTT+TGG | - | Chr1:67806810-67806829 | MsG0180003762.01.T01:intron | 25.0% |
| !!! | TGGTTTTGAAACCTTTTTTT+GGG | - | Chr1:67807238-67807257 | MsG0180003762.01.T01:CDS | 25.0% |
| !!! | TTGGTTTTGAAACCTTTTTT+TGG | - | Chr1:67807237-67807256 | MsG0180003762.01.T01:CDS | 25.0% |
| !!! | TTGTGATTTTGTTTATTTCG+AGG | - | Chr1:67806600-67806619 | MsG0180003762.01.T01:CDS | 25.0% |
| AATCTTGTCAACTATCCAAA+CGG | + | Chr1:67806800-67806819 | None:intergenic | 30.0% | |
| ACTAATTCCATTTCCAAAAG+GGG | + | Chr1:67807354-67807373 | None:intergenic | 30.0% | |
| ATACTCAACTTCATTTCCAT+TGG | + | Chr1:67806180-67806199 | None:intergenic | 30.0% | |
| CATGCTTGTGATTATAAATC+AGG | - | Chr1:67806293-67806312 | MsG0180003762.01.T01:intron | 30.0% | |
| GATATTCAGGAAATTCTTGT+GGG | + | Chr1:67806025-67806044 | None:intergenic | 30.0% | |
| GCTAAATATGATGAGACTAA+TGG | - | Chr1:67806131-67806150 | MsG0180003762.01.T01:intron | 30.0% | |
| GGAATTAAGAGGTTTACTAA+TGG | - | Chr1:67806982-67807001 | MsG0180003762.01.T01:intron | 30.0% | |
| GTAGTTGACAGAATTGTTTA+GGG | + | Chr1:67805989-67806008 | None:intergenic | 30.0% | |
| TGCAAATTTGATGTTTGGTT+CGG | - | Chr1:67807201-67807220 | MsG0180003762.01.T01:CDS | 30.0% | |
| TGTGATTATAAATCAGGTGA+AGG | - | Chr1:67806299-67806318 | MsG0180003762.01.T01:intron | 30.0% | |
| TGTTTCTTTGTTGGATATTC+AGG | + | Chr1:67806038-67806057 | None:intergenic | 30.0% | |
| TTAGTAAACCTCTTAATTCC+AGG | + | Chr1:67806982-67807001 | None:intergenic | 30.0% | |
| ! | TATCTTCTTCATTATCGACT+AGG | + | Chr1:67805788-67805807 | None:intergenic | 30.0% |
| ! | TTTTGCCTAGAGAAGTTTTT+GGG | - | Chr1:67806731-67806750 | MsG0180003762.01.T01:intron | 30.0% |
| ! | TTTTTACCTTTTTCCCCTTT+TGG | - | Chr1:67807338-67807357 | MsG0180003762.01.T01:CDS | 30.0% |
| !! | GTTTTTGGGAAATCAACATT+TGG | - | Chr1:67806745-67806764 | MsG0180003762.01.T01:intron | 30.0% |
| !! | TTTGGTTCATTTTTGTGTGT+AGG | - | Chr1:67807413-67807432 | MsG0180003762.01.T01:CDS | 30.0% |
| !! | TTTTAGAGGGAAGAAAGTTT+TGG | - | Chr1:67806322-67806341 | MsG0180003762.01.T01:intron | 30.0% |
| !!! | AGATTCTTTTGATTTTGGCT+TGG | - | Chr1:67806815-67806834 | MsG0180003762.01.T01:intron | 30.0% |
| !!! | GGTCGAAGATTTTTAAGTTT+GGG | - | Chr1:67806570-67806589 | MsG0180003762.01.T01:CDS | 30.0% |
| !!! | GTCGAAGATTTTTAAGTTTG+GGG | - | Chr1:67806571-67806590 | MsG0180003762.01.T01:CDS | 30.0% |
| !!! | TGGTCGAAGATTTTTAAGTT+TGG | - | Chr1:67806569-67806588 | MsG0180003762.01.T01:CDS | 30.0% |
| ACAGTTGAACCATGATTCAT+AGG | - | Chr1:67807175-67807194 | MsG0180003762.01.T01:CDS | 35.0% | |
| ACCTTGGAGAAAATTAGATC+TGG | - | Chr1:67806940-67806959 | MsG0180003762.01.T01:intron | 35.0% | |
| ATCTTGTCAACTATCCAAAC+GGG | + | Chr1:67806799-67806818 | None:intergenic | 35.0% | |
| CTCAAGATATTGCTCAAGTT+TGG | - | Chr1:67807571-67807590 | MsG0180003762.01.T01:CDS | 35.0% | |
| GAAGGGTTGAGTAAATTCAA+AGG | - | Chr1:67806260-67806279 | MsG0180003762.01.T01:intron | 35.0% | |
| GATTTCCCAAAAACTTCTCT+AGG | + | Chr1:67806739-67806758 | None:intergenic | 35.0% | |
| GGATATTCAGGAAATTCTTG+TGG | + | Chr1:67806026-67806045 | None:intergenic | 35.0% | |
| GGTAGTTGACAGAATTGTTT+AGG | + | Chr1:67805990-67806009 | None:intergenic | 35.0% | |
| GGTCTTGCAAATTTGATGTT+TGG | - | Chr1:67807196-67807215 | MsG0180003762.01.T01:CDS | 35.0% | |
| GTAAACGGTCCTATAATAGT+AGG | - | Chr1:67805831-67805850 | MsG0180003762.01.T01:CDS | 35.0% | |
| TGGGAAATCAACATTTGGAT+TGG | - | Chr1:67806750-67806769 | MsG0180003762.01.T01:intron | 35.0% | |
| TTACTCAACCCTTCAATATC+AGG | + | Chr1:67806254-67806273 | None:intergenic | 35.0% | |
| TTCTATTGTTCTTCGTAGCT+CGG | - | Chr1:67806409-67806428 | MsG0180003762.01.T01:CDS | 35.0% | |
| TTGGAGCTTAAGAACACAAA+GGG | - | Chr1:67806892-67806911 | MsG0180003762.01.T01:intron | 35.0% | |
| TTTGCAAGACCTATGAATCA+TGG | + | Chr1:67807187-67807206 | None:intergenic | 35.0% | |
| TTTGTGTTCTTAAGCTCCAA+AGG | + | Chr1:67806892-67806911 | None:intergenic | 35.0% | |
| ! | ACCAGATCTAATTTTCTCCA+AGG | + | Chr1:67806944-67806963 | None:intergenic | 35.0% |
| ! | GGTGAATTTTTCTCCAAGAA+TGG | - | Chr1:67807438-67807457 | MsG0180003762.01.T01:CDS | 35.0% |
| ! | GTTTTGCCTAGAGAAGTTTT+TGG | - | Chr1:67806730-67806749 | MsG0180003762.01.T01:intron | 35.0% |
| ! | TGACTTTGGAAATCCATTCT+TGG | + | Chr1:67807454-67807473 | None:intergenic | 35.0% |
| ! | TGGCAGTTATGATGATGAAA+TGG | - | Chr1:67806770-67806789 | MsG0180003762.01.T01:intron | 35.0% |
| ! | TTTGGAGCTTAAGAACACAA+AGG | - | Chr1:67806891-67806910 | MsG0180003762.01.T01:intron | 35.0% |
| !! | AAATCAGGTGAAGGTTTTAG+AGG | - | Chr1:67806308-67806327 | MsG0180003762.01.T01:intron | 35.0% |
| !! | GTTCATTTTTGTGTGTAGGA+AGG | - | Chr1:67807417-67807436 | MsG0180003762.01.T01:CDS | 35.0% |
| !! | TTCAACTGTTTTGAACTGCT+CGG | + | Chr1:67807164-67807183 | None:intergenic | 35.0% |
| !! | TTGCTCTGAAAGAACTAATG+TGG | - | Chr1:67806549-67806568 | MsG0180003762.01.T01:CDS | 35.0% |
| !!! | AAAGTTTTGGTTGTTGGTTG+TGG | - | Chr1:67806335-67806354 | MsG0180003762.01.T01:CDS | 35.0% |
| !!! | AATCAGGTGAAGGTTTTAGA+GGG | - | Chr1:67806309-67806328 | MsG0180003762.01.T01:intron | 35.0% |
| !!! | ATTTTGGCTTGGTTAGTGTT+TGG | - | Chr1:67806826-67806845 | MsG0180003762.01.T01:intron | 35.0% |
| !!! | TGTTGTGTTTTTGGTTGAAC+AGG | - | Chr1:67806702-67806721 | MsG0180003762.01.T01:intron | 35.0% |
| AAGTTGTGCCTGGAATTAAG+AGG | - | Chr1:67806971-67806990 | MsG0180003762.01.T01:intron | 40.0% | |
| AATGGTTGGAAAGGAACTGT+TGG | - | Chr1:67807483-67807502 | MsG0180003762.01.T01:CDS | 40.0% | |
| ACAGTTCCTTTCCAACCATT+AGG | + | Chr1:67807483-67807502 | None:intergenic | 40.0% | |
| ACTGTTGGACTTTATGCTGT+TGG | - | Chr1:67807498-67807517 | MsG0180003762.01.T01:CDS | 40.0% | |
| AGAGTTGAGACTGTTTCCAA+TGG | - | Chr1:67806161-67806180 | MsG0180003762.01.T01:intron | 40.0% | |
| AGGTGCCAATATCCAAAACA+GGG | + | Chr1:67806924-67806943 | None:intergenic | 40.0% | |
| AGTCTCACATCGTTGCATTT+GGG | - | Chr1:67805809-67805828 | MsG0180003762.01.T01:CDS | 40.0% | |
| CAAATTGCATAGCTTCACTG+TGG | - | Chr1:67805928-67805947 | MsG0180003762.01.T01:CDS | 40.0% | |
| CAGTTCCTTTCCAACCATTA+GGG | + | Chr1:67807482-67807501 | None:intergenic | 40.0% | |
| CATCGTTGCATTTGGGTAAA+CGG | - | Chr1:67805816-67805835 | MsG0180003762.01.T01:CDS | 40.0% | |
| CCATTTCCAAAAGGGGAAAA+AGG | + | Chr1:67807347-67807366 | None:intergenic | 40.0% | |
| CTGTTGGACTTTATGCTGTT+GGG | - | Chr1:67807499-67807518 | MsG0180003762.01.T01:CDS | 40.0% | |
| CTGTTGGGTTTACAAAGAGA+GGG | - | Chr1:67807514-67807533 | MsG0180003762.01.T01:CDS | 40.0% | |
| GAGCTACGAAGAACAATAGA+AGG | + | Chr1:67806410-67806429 | None:intergenic | 40.0% | |
| GATCTTTCAAGCACCATGAA+GGG | + | Chr1:67805909-67805928 | None:intergenic | 40.0% | |
| GCAGCATGTCTTAAACAACA+AGG | - | Chr1:67805879-67805898 | MsG0180003762.01.T01:CDS | 40.0% | |
| GTATATTTGTAGGTCGCTTG+TGG | - | Chr1:67806196-67806215 | MsG0180003762.01.T01:intron | 40.0% | |
| GTGTTGTGCCTGATATTGAA+GGG | - | Chr1:67806243-67806262 | MsG0180003762.01.T01:intron | 40.0% | |
| GTTGACAGAATTGTTTAGGG+AGG | + | Chr1:67805986-67806005 | None:intergenic | 40.0% | |
| TAGTCTCACATCGTTGCATT+TGG | - | Chr1:67805808-67805827 | MsG0180003762.01.T01:CDS | 40.0% | |
| TCAGGAAATTCTTGTGGGAA+TGG | + | Chr1:67806020-67806039 | None:intergenic | 40.0% | |
| TCTTGTGGGAATGGAAAGTT+AGG | + | Chr1:67806011-67806030 | None:intergenic | 40.0% | |
| TGATCTTTCAAGCACCATGA+AGG | + | Chr1:67805910-67805929 | None:intergenic | 40.0% | |
| TGATGAAATGGTTACCCGTT+TGG | - | Chr1:67806782-67806801 | MsG0180003762.01.T01:intron | 40.0% | |
| TGTGTTGTGCCTGATATTGA+AGG | - | Chr1:67806242-67806261 | MsG0180003762.01.T01:intron | 40.0% | |
| TTCTCACCTTAAGCCAAGAA+GGG | + | Chr1:67807083-67807102 | None:intergenic | 40.0% | |
| TTTCTCACCTTAAGCCAAGA+AGG | + | Chr1:67807084-67807103 | None:intergenic | 40.0% | |
| ! | AAGGTGCCAATATCCAAAAC+AGG | + | Chr1:67806925-67806944 | None:intergenic | 40.0% |
| ! | GTATGGTCTTAAAAGGCCAT+TGG | - | Chr1:67806861-67806880 | MsG0180003762.01.T01:intron | 40.0% |
| ! | GTTGTTGGTTGTGGTAATTC+TGG | - | Chr1:67806344-67806363 | MsG0180003762.01.T01:CDS | 40.0% |
| ! | TGGTTGTGGTAATTCTGGAA+TGG | - | Chr1:67806349-67806368 | MsG0180003762.01.T01:CDS | 40.0% |
| !! | AAGACCCCTGTTTTGGATAT+TGG | - | Chr1:67806916-67806935 | MsG0180003762.01.T01:intron | 40.0% |
| !! | ATGATGAGACTAATGGGTTG+TGG | - | Chr1:67806138-67806157 | MsG0180003762.01.T01:intron | 40.0% |
| !! | CCTTTTTCCCCTTTTGGAAA+TGG | - | Chr1:67807344-67807363 | MsG0180003762.01.T01:CDS | 40.0% |
| !! | GGCTGTGTTGAACTTGTTAA+TGG | - | Chr1:67807003-67807022 | MsG0180003762.01.T01:CDS | 40.0% |
| !! | TGTTTTGGATATTGGCACCT+TGG | - | Chr1:67806924-67806943 | MsG0180003762.01.T01:intron | 40.0% |
| !!! | GGGAAGAAAGTTTTGGTTGT+TGG | - | Chr1:67806329-67806348 | MsG0180003762.01.T01:intron | 40.0% |
| !!! | GTTCGGTTTTGAAACATCGT+TGG | - | Chr1:67807218-67807237 | MsG0180003762.01.T01:CDS | 40.0% |
| AGTCACCATTCCCTAATGGT+TGG | - | Chr1:67807469-67807488 | MsG0180003762.01.T01:CDS | 45.0% | |
| ATCGTAGCAATGTCCCTTCT+TGG | - | Chr1:67807067-67807086 | MsG0180003762.01.T01:CDS | 45.0% | |
| CAATGTCCCTTCTTGGCTTA+AGG | - | Chr1:67807074-67807093 | MsG0180003762.01.T01:CDS | 45.0% | |
| CCAAAGTCACCATTCCCTAA+TGG | - | Chr1:67807465-67807484 | MsG0180003762.01.T01:CDS | 45.0% | |
| CCATTAGGGAATGGTGACTT+TGG | + | Chr1:67807468-67807487 | None:intergenic | 45.0% | |
| CCATTCCCTAATGGTTGGAA+AGG | - | Chr1:67807474-67807493 | MsG0180003762.01.T01:CDS | 45.0% | |
| CCTTTCCAACCATTAGGGAA+TGG | + | Chr1:67807477-67807496 | None:intergenic | 45.0% | |
| GAACACACTCGTTGAATCGT+GGG | + | Chr1:67806106-67806125 | None:intergenic | 45.0% | |
| GATGCAGTTGTTCTTGCTAC+TGG | - | Chr1:67807042-67807061 | MsG0180003762.01.T01:CDS | 45.0% | |
| GCTGTTGGGTTTACAAAGAG+AGG | - | Chr1:67807513-67807532 | MsG0180003762.01.T01:CDS | 45.0% | |
| GGTCACATAAAAGTTGTGCC+TGG | - | Chr1:67806961-67806980 | MsG0180003762.01.T01:intron | 45.0% | |
| GGTCCTATAATAGTAGGAGC+AGG | - | Chr1:67805837-67805856 | MsG0180003762.01.T01:CDS | 45.0% | |
| GGTCCTGCTCCTACTATTAT+AGG | + | Chr1:67805843-67805862 | None:intergenic | 45.0% | |
| GGTCTTAAAAGGCCATTGGA+GGG | - | Chr1:67806865-67806884 | MsG0180003762.01.T01:intron | 45.0% | |
| GGTGCCAATATCCAAAACAG+GGG | + | Chr1:67806923-67806942 | None:intergenic | 45.0% | |
| TGAACACACTCGTTGAATCG+TGG | + | Chr1:67806107-67806126 | None:intergenic | 45.0% | |
| TTTACAAAGAGAGGGCTCTC+TGG | - | Chr1:67807522-67807541 | MsG0180003762.01.T01:CDS | 45.0% | |
| ! | AAAGGGTAAGACCCCTGTTT+TGG | - | Chr1:67806909-67806928 | MsG0180003762.01.T01:intron | 45.0% |
| ! | GATTTCGAAGTGTTTCGCGT+AGG | + | Chr1:67806081-67806100 | None:intergenic | 45.0% |
| ! | TGGTCTTAAAAGGCCATTGG+AGG | - | Chr1:67806864-67806883 | MsG0180003762.01.T01:intron | 45.0% |
| !! | ACAACAAGGAGTACCCTTCA+TGG | - | Chr1:67805893-67805912 | MsG0180003762.01.T01:CDS | 45.0% |
| !!! | AAAAAAATTATTTTATGTAT+TGG | + | Chr1:67807291-67807310 | None:intergenic | 5.0% |
| ATAGTAGGAGCAGGACCATC+AGG | - | Chr1:67805846-67805865 | MsG0180003762.01.T01:CDS | 50.0% | |
| AAGGCCATTGGAGGGTCCTT+TGG | - | Chr1:67806873-67806892 | MsG0180003762.01.T01:intron | 55.0% | |
| AGCTCCAAAGGACCCTCCAA+TGG | + | Chr1:67806880-67806899 | None:intergenic | 55.0% | |
| AGGTCGCTTGTGGTTGCTAC+TGG | - | Chr1:67806206-67806225 | MsG0180003762.01.T01:intron | 55.0% | |
| GCTGCTGTTGCTAGACCTGA+TGG | + | Chr1:67805864-67805883 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 67805768 | 67807662 | 67805768 | ID=MsG0180003762.01;Name=MsG0180003762.01 |
| Chr1 | mRNA | 67805768 | 67807662 | 67805768 | ID=MsG0180003762.01.T01;Parent=MsG0180003762.01;Name=MsG0180003762.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|3|0|420 |
| Chr1 | exon | 67807000 | 67807662 | 67807000 | ID=MsG0180003762.01.T01:exon:51727;Parent=MsG0180003762.01.T01 |
| Chr1 | exon | 67806335 | 67806706 | 67806335 | ID=MsG0180003762.01.T01:exon:51726;Parent=MsG0180003762.01.T01 |
| Chr1 | exon | 67805768 | 67805995 | 67805768 | ID=MsG0180003762.01.T01:exon:51725;Parent=MsG0180003762.01.T01 |
| Chr1 | CDS | 67807000 | 67807662 | 67807000 | ID=MsG0180003762.01.T01:cds;Parent=MsG0180003762.01.T01 |
| Chr1 | CDS | 67806335 | 67806706 | 67806335 | ID=MsG0180003762.01.T01:cds;Parent=MsG0180003762.01.T01 |
| Chr1 | CDS | 67805768 | 67805995 | 67805768 | ID=MsG0180003762.01.T01:cds;Parent=MsG0180003762.01.T01 |
| Gene Sequence |
| Protein sequence |
