Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035214.01.T01 | XP_039682635.1 | 77.233 | 571 | 79 | 5 | 1 | 521 | 1 | 570 | 0 | 842 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035214.01.T01 | MTR_6g077540 | 77.233 | 571 | 79 | 5 | 1 | 521 | 1 | 570 | 0.0 | 842 |
| MsG0680035214.01.T01 | MTR_6g065540 | 73.250 | 557 | 113 | 2 | 1 | 521 | 1 | 557 | 0.0 | 811 |
| MsG0680035214.01.T01 | MTR_6g079920 | 72.598 | 562 | 104 | 4 | 9 | 520 | 10 | 571 | 0.0 | 797 |
| MsG0680035214.01.T01 | MTR_6g079920 | 29.534 | 386 | 237 | 1 | 70 | 420 | 191 | 576 | 3.63e-58 | 203 |
| MsG0680035214.01.T01 | MTR_6g079920 | 26.425 | 193 | 142 | 0 | 51 | 243 | 382 | 574 | 1.88e-20 | 95.1 |
| MsG0680035214.01.T01 | MTR_6g079830 | 72.598 | 562 | 104 | 4 | 9 | 520 | 10 | 571 | 0.0 | 796 |
| MsG0680035214.01.T01 | MTR_6g079830 | 31.233 | 365 | 251 | 0 | 53 | 417 | 209 | 573 | 2.46e-56 | 199 |
| MsG0680035214.01.T01 | MTR_6g079830 | 30.564 | 337 | 234 | 0 | 50 | 386 | 241 | 577 | 7.34e-54 | 192 |
| MsG0680035214.01.T01 | MTR_6g079830 | 24.390 | 246 | 181 | 1 | 37 | 277 | 328 | 573 | 3.59e-15 | 79.0 |
| MsG0680035214.01.T01 | MTR_6g079310 | 75.050 | 501 | 90 | 1 | 56 | 521 | 1 | 501 | 0.0 | 756 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035214.01.T01 | AT1G62910 | 35.727 | 543 | 279 | 3 | 48 | 520 | 62 | 604 | 4.27e-120 | 367 |
| MsG0680035214.01.T01 | AT1G62910 | 29.056 | 413 | 293 | 0 | 108 | 520 | 52 | 464 | 1.45e-66 | 227 |
| MsG0680035214.01.T01 | AT1G62910 | 29.676 | 401 | 272 | 4 | 49 | 446 | 238 | 631 | 2.02e-60 | 210 |
| MsG0680035214.01.T01 | AT1G62910 | 32.698 | 315 | 212 | 0 | 207 | 521 | 81 | 395 | 4.99e-53 | 190 |
| MsG0680035214.01.T01 | AT1G62670 | 35.413 | 545 | 282 | 3 | 46 | 520 | 58 | 602 | 2.25e-119 | 365 |
| MsG0680035214.01.T01 | AT1G62670 | 29.146 | 398 | 278 | 2 | 49 | 446 | 236 | 629 | 9.04e-61 | 211 |
| MsG0680035214.01.T01 | AT1G62670 | 30.794 | 315 | 218 | 0 | 207 | 521 | 79 | 393 | 8.60e-48 | 176 |
| MsG0680035214.01.T01 | AT1G62670 | 35.413 | 545 | 282 | 3 | 46 | 520 | 58 | 602 | 2.25e-119 | 365 |
| MsG0680035214.01.T01 | AT1G62670 | 29.146 | 398 | 278 | 2 | 49 | 446 | 236 | 629 | 9.04e-61 | 211 |
| MsG0680035214.01.T01 | AT1G62670 | 30.794 | 315 | 218 | 0 | 207 | 521 | 79 | 393 | 8.60e-48 | 176 |
Find 88 sgRNAs with CRISPR-Local
Find 112 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAACAGTGTGGTAATGTT+TGG | 0.361795 | 6:-100690515 | None:intergenic |
| AACACGTCTCAGCAATTCAA+GGG | 0.621865 | 6:-100690848 | None:intergenic |
| AACCTTCACTACACTCATCA+AGG | 0.486997 | 6:+100690690 | MsG0680035214.01.T01:CDS |
| AAGAAGGAATGAAATAGAAA+TGG | 0.363985 | 6:-100690363 | None:intergenic |
| AATTCGATTGCAGGTGGAGT+GGG | 0.537316 | 6:-100690463 | None:intergenic |
| AATTGCTGAGACGTGTTGAT+GGG | 0.586300 | 6:+100690854 | MsG0680035214.01.T01:CDS |
| ACAAAACCCATGGATCATAA+CGG | 0.595085 | 6:-100691643 | None:intergenic |
| ACATACACTATTCTTATTGA+TGG | 0.276400 | 6:+100691531 | MsG0680035214.01.T01:CDS |
| ACATATACCGTTATGATCCA+TGG | 0.485344 | 6:+100691636 | MsG0680035214.01.T01:CDS |
| ACATATTTGGTCGAATACCC+TGG | 0.610365 | 6:-100691506 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACTTACAACTCTTTAATGGA+TGG | + | Chr6:100691111-100691130 | MsG0680035214.01.T01:CDS | 30.0% | |
| ACTTACAATTCCCTTATTGA+TGG | + | Chr6:100691321-100691340 | MsG0680035214.01.T01:CDS | 30.0% | |
| ACTTACAGTGCTTTAATTAG+TGG | + | Chr6:100691006-100691025 | MsG0680035214.01.T01:CDS | 30.0% | |
| AGTTATGGAACCTTGATTAA+TGG | + | Chr6:100690796-100690815 | MsG0680035214.01.T01:CDS | 30.0% | |
| ATACTGTAACTGTGAATATC+AGG | - | Chr6:100691207-100691226 | None:intergenic | 30.0% | |
| CAGAAAAATTATTCCTGATG+TGG | + | Chr6:100691296-100691315 | MsG0680035214.01.T01:CDS | 30.0% | |
| CATTGTTATCAAACAGAGAA+CGG | - | Chr6:100691761-100691780 | None:intergenic | 30.0% | |
| CATTTAGACCAAGTTAGTTA+TGG | + | Chr6:100690781-100690800 | MsG0680035214.01.T01:CDS | 30.0% | |
| CATTTCTTCAAAGAGATTCA+TGG | - | Chr6:100691271-100691290 | None:intergenic | 30.0% | |
| CCTAGTTAAAAAAGTGAACA+AGG | + | Chr6:100691140-100691159 | MsG0680035214.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 100690271 | 100691836 | 100690271 | ID=MsG0680035214.01;Name=MsG0680035214.01 |
| Chr6 | mRNA | 100690271 | 100691836 | 100690271 | ID=MsG0680035214.01.T01;Parent=MsG0680035214.01;Name=MsG0680035214.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|521 |
| Chr6 | exon | 100690271 | 100691836 | 100690271 | ID=MsG0680035214.01.T01:exon:20638;Parent=MsG0680035214.01.T01 |
| Chr6 | CDS | 100690271 | 100691836 | 100690271 | ID=MsG0680035214.01.T01:cds;Parent=MsG0680035214.01.T01 |
| Gene Sequence |
>MsG0680035214.01.T01 ATGTTTCATTCTTCTTCTTCTTCGATGTTGCAGAGGTATTGTGTTGTTCCTGTTTCAAAAAAGTTTCATCCTTTTCAATTTCTGAATAACACCCATTTCTATTTCATTCCTTCTTTTTGCTTTTATTCTTCAACTTCAAACATTGATGTTGATGATGCTGTTTCATTGTTCAATCGCTTGCTCCGTAAGAATCCCACTCCACCTGCAATCGAATTCGGTAAGATTTTAGGTTCCCTTGTCAAATCCAAACATTACCACACTGTTGTTTCTGTTTCTCAAAAAATGGAATTTGAGGGAATTAAACTTGACTTAGTCAATTGCAACATCCTTATCAATTCTTTCTGTCAATTGGGTCTTATCCCTTTTGCTTTTTCTGTTTTCGCAAAGATTCTCAAAAAGGGTTATCATCCCAATACAATAACCTTCACTACACTCATCAAGGGTCACTGTCTAAAAGGTCAGATACATCAAGCATTGAACTTACATGATAAGGTGATTGCGATGGGATTTCATTTAGACCAAGTTAGTTATGGAACCTTGATTAATGGGTTGTGTAAAGTTGGTGAAACAAAAGCAGCCCTTGAATTGCTGAGACGTGTTGATGGGAAATTGGTTCAGCCTAATGTGGTAATGTACAATACGATTATTGATGGTATGTGCAAAGGTAAACATGTTAATGATGCTTTTGATTTATATTCTGAAATGGTTTCTAAGGGGATTTCTCTCGATGTTGTTACTTACAGTGCTTTAATTAGTGGCTTTTGCATTGTTGGTAAAATGAAAGATGCAATTGATTTGTTTCATAAAATGATATTAGAAAACATCAACCCAAATGTGTATACTTACAACTCTTTAATGGATGGATATTGCCTAGTTAAAAAAGTGAACAAGGCGAAGAGTATATTCAACACTATGGCTCAGGGGGGAGTGAATCCTGATATTCACAGTTACAGTATCTTGATTAATGGGTTTTGTAAGATTAAAATGATGGATGAAGCCATGAATCTCTTTGAAGAAATGCATTGCAGAAAAATTATTCCTGATGTGGTAACTTACAATTCCCTTATTGATGGTTTGTGTAAATCGGGGAAAATCTCTTATGCTTTGAAGCTAGTCGATGAGATGCATGATAGAGGTCAAGCACCTGATATGATTACTTACAATTCTATATTGGATGCTTTGTGCAAAAACCATCAGGTTGACAGGGCGATTGCATTATTAACAAAATTGAAAGACCAGGGTATTCGACCAAATATGTACACATACACTATTCTTATTGATGGATTGTGTAAAGGTGGAAGACTAGAGGATGCACGAAATGTTTTTGAGGATCTTTTAGTTAAAGGCTATAATATAACTGTCAACACATATACCGTTATGATCCATGGGTTTTGTAACAAGGGCTTGTTTGATGAAGCATTGGCTTTGTTGTCAAAAATGAAAGACAATAGTTGCATTCCAGACGCCGTTACTTACAAAATAATCATCCGTTCTCTGTTTGATAACAATGAAAATGATAAGGCGGAGAAACTTCTTTGTGAAATGATTACAAGAGGTCTATTTTAA |
| Protein sequence |
>MsG0680035214.01.T01 MFHSSSSSMLQRYCVVPVSKKFHPFQFLNNTHFYFIPSFCFYSSTSNIDVDDAVSLFNRLLRKNPTPPAIEFGKILGSLVKSKHYHTVVSVSQKMEFEGIKLDLVNCNILINSFCQLGLIPFAFSVFAKILKKGYHPNTITFTTLIKGHCLKGQIHQALNLHDKVIAMGFHLDQVSYGTLINGLCKVGETKAALELLRRVDGKLVQPNVVMYNTIIDGMCKGKHVNDAFDLYSEMVSKGISLDVVTYSALISGFCIVGKMKDAIDLFHKMILENINPNVYTYNSLMDGYCLVKKVNKAKSIFNTMAQGGVNPDIHSYSILINGFCKIKMMDEAMNLFEEMHCRKIIPDVVTYNSLIDGLCKSGKISYALKLVDEMHDRGQAPDMITYNSILDALCKNHQVDRAIALLTKLKDQGIRPNMYTYTILIDGLCKGGRLEDARNVFEDLLVKGYNITVNTYTVMIHGFCNKGLFDEALALLSKMKDNSCIPDAVTYKIIIRSLFDNNENDKAEKLLCEMITRGLF* |
