AlfalfaGEDB | MsG0680035264.01

M. sativa cultivar ZhongmuNo.1 / MsG0680035264.01

KEGG: -
GO: GO:0005515
PFAM: PF12854 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035264.01.T01	XP_039691065.1	76.155	541	103	3	4	523	6	541	0	834

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035264.01.T01	Q9LPX2	34.011	541	301	4	37	522	70	609	2.51E-115	357

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035264.01.T01	A0A396HH44	75.231	541	109	4	4	523	19	555	0.0	834

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048802.01	MsG0680035264.01	PPI
MsG0080048802.01	MsG0680035264.01	PPI
MsG0080048802.01	MsG0680035264.01	PPI
MsG0080048925.01	MsG0680035264.01	PPI
MsG0080048926.01	MsG0680035264.01	PPI
MsG0180001046.01	MsG0680035264.01	PPI
MsG0180001047.01	MsG0680035264.01	PPI
MsG0180001883.01	MsG0680035264.01	PPI
MsG0180001883.01	MsG0680035264.01	PPI
MsG0180001883.01	MsG0680035264.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035264.01.T01	MTR_0110s0060	73.198	541	124	2	4	523	2	542	0.0	809
MsG0680035264.01.T01	MTR_6g079440	74.157	534	117	2	10	523	9	541	0.0	808
MsG0680035264.01.T01	MTR_6g069300	71.875	544	130	3	1	521	6	549	0.0	795
MsG0680035264.01.T01	MTR_6g069300	29.730	333	211	4	212	522	44	375	3.43e-41	157
MsG0680035264.01.T01	MTR_6g080210	68.347	496	117	4	4	460	2	496	0.0	682
MsG0680035264.01.T01	MTR_6g080210	28.375	363	240	3	163	522	56	401	3.10e-48	174
MsG0680035264.01.T01	MTR_6g080210	28.571	182	127	2	47	226	326	506	2.43e-19	91.7
MsG0680035264.01.T01	MTR_0308s0020	64.030	531	168	3	15	523	12	541	0.0	682
MsG0680035264.01.T01	MTR_6g068820	68.607	481	116	2	49	523	1	452	0.0	669
MsG0680035264.01.T01	MTR_6g068820	29.846	325	213	2	38	362	137	446	7.77e-45	164

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680035264.01.T01	AT1G12775	34.011	541	301	4	37	522	70	609	2.55e-116	357
MsG0680035264.01.T01	AT1G12775	29.337	392	277	0	86	477	223	614	6.45e-63	217
MsG0680035264.01.T01	AT1G12775	27.179	390	284	0	59	448	231	620	8.00e-55	195
MsG0680035264.01.T01	AT1G12775	27.441	379	274	1	47	425	254	631	4.50e-54	193
MsG0680035264.01.T01	AT1G12775	27.624	362	247	1	162	523	89	435	1.95e-49	180
MsG0680035264.01.T01	AT1G12700	35.897	507	302	4	37	522	86	590	2.78e-116	357
MsG0680035264.01.T01	AT1G12700	24.201	438	323	3	12	448	172	601	1.33e-53	191
MsG0680035264.01.T01	AT1G12700	28.378	370	265	0	47	416	235	604	5.72e-52	187
MsG0680035264.01.T01	AT1G12700	28.895	353	225	4	194	523	102	451	1.24e-44	166
MsG0680035264.01.T01	AT1G12700	22.899	345	265	1	37	381	261	604	4.19e-33	133

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Find 93 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTAGCTTGAGAATCT+TGG	0.357947	6:-101225172	None:intergenic
AACATCGTCATGAAAGTAAA+GGG	0.453277	6:-101225262	None:intergenic
AAGAAGAAACGAATTGGGAG+TGG	0.478388	6:-101224863	None:intergenic
AAGAATGTTGAATACATGAT+TGG	0.471118	6:-101225787	None:intergenic
AAGCGACATAAATGATAAGG+CGG	0.698511	6:+101226398	MsG0680035264.01.T01:CDS
AATCTTACCAAATTCGAAGA+TGG	0.413188	6:-101224992	None:intergenic
AATCTTTCTAAGAACCTGCA+AGG	0.624944	6:-101225367	None:intergenic
AATTCGAAGATGGGTTGGGT+AGG	0.630955	6:-101224982	None:intergenic
ACACTGTAGCTATGAACATT+AGG	0.435376	6:-101225828	None:intergenic
ACATACAGTTCACTTATTGA+TGG	0.382345	6:+101225945	MsG0680035264.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACATCGTCATGAAAGTAAA+GGG	-	Chr6:101225265-101225284	None:intergenic	30.0%
AATCTTACCAAATTCGAAGA+TGG	-	Chr6:101224995-101225014	None:intergenic	30.0%
ACATACAGTTCACTTATTGA+TGG	+	Chr6:101225945-101225964	MsG0680035264.01.T01:CDS	30.0%
ACCTTAACTACACTTATCAA+AGG	+	Chr6:101225210-101225229	MsG0680035264.01.T01:CDS	30.0%
ACCTTTGATAAGTGTAGTTA+AGG	-	Chr6:101225214-101225233	None:intergenic	30.0%
ACTTATACTTCTTTGATGGA+TGG	+	Chr6:101225735-101225754	MsG0680035264.01.T01:CDS	30.0%
AGCATCCAATAAAGAATTGT+AGG	-	Chr6:101226054-101226073	None:intergenic	30.0%
ATAGATGAAATGCATAACAG+AGG	+	Chr6:101226008-101226027	MsG0680035264.01.T01:CDS	30.0%
ATAGTGTACGTATACATATC+TGG	-	Chr6:101226146-101226165	None:intergenic	30.0%
ATCAAAGGTCTTTGTCTTAA+TGG	+	Chr6:101225225-101225244	MsG0680035264.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	101224829	101226461	101224829	ID=MsG0680035264.01;Name=MsG0680035264.01
Chr6	mRNA	101224829	101226461	101224829	ID=MsG0680035264.01.T01;Parent=MsG0680035264.01;Name=MsG0680035264.01.T01;_AED=0.46;_eAED=0.46;_QI=0\|0\|0\|1\|1\|1\|2\|0\|523
Chr6	exon	101224829	101226232	101224829	ID=MsG0680035264.01.T01:exon:20696;Parent=MsG0680035264.01.T01
Chr6	exon	101226294	101226461	101226294	ID=MsG0680035264.01.T01:exon:20697;Parent=MsG0680035264.01.T01
Chr6	CDS	101224829	101226232	101224829	ID=MsG0680035264.01.T01:cds;Parent=MsG0680035264.01.T01
Chr6	CDS	101226294	101226461	101226294	ID=MsG0680035264.01.T01:cds;Parent=MsG0680035264.01.T01

Gene Sequence

>MsG0680035264.01.T01

ATGAGAAAGTTAAGGTATGCAAATCTCTCTCTTCCCACTCCCAATTCGTTTCTTCTTCTTCATCGTGGATTACACATTCAGCCTCTTCCTTCGTTCATTGATAATTTTGATGATGCTGTTTCATCATTCAATCACATTCTCCATATGAAGAATCCTACCCAACCCATCTTCGAATTTGGTAAGATTTTATCGTCCCTTGTTAAGTTGAATCATTTCAACACCGTTATTTCATTTTCTCATCAAATGGAAATCAAAGGAATTCAACCTAACATTGTTATTTTAAACATCTTGATTAATTCTTTCTGCCATTTGAATCAACTCAATTTTGCATTTTCTGTAATTGCCAAGATTCTCAAGCTAGGTTTTCAACCTGATACTGTAACCTTAACTACACTTATCAAAGGTCTTTGTCTTAATGGCGAGGTTAGGAAAGCCCTTTACTTTCATGACGATGTTATAGCAATGGGATTTCATCTTAATCATGTTAGTTATGGGACATTGATCAACGGGTTATGTAAATCCGGAGAAACAAAAGCTGCCTTGCAGGTTCTTAGAAAGATTGAAGAGAAATGTGTTAGACCTGACGTAGTAATGTACAACACTATTATGGATAGTCTATGCAAGAATAAATTAGTAATTGATGCGTGTGATTTATATTCTGAAATGCTTGTTAGAAGAATTTCTCCTGATGTGATCACTTACAGTACTTTAATACATGGTTTTTGCATTGTGGGTCAAATGAAAGAAGCAGTTGGTTTGTTAAATGAAATGACATTGAAAAACATCACCCCGAATGTTCGTACTTTTAATACATTGGTTGATGGTTTATGTAAGGAAGGAGAGATGAAAAAAGCTACAAGTGTGTTACCTGTTATGATAAAACAAGGTTTGGAACCTAATCTTGTTACTTATACTTCTTTGATGGATGGGTATTTCTTGGTAAAAGAAGTCAACAAGGCCAATCATGTATTCAACATTCTTTCTCTAAGGGGAGTGACTCCTAATGTTCATAGCTACAGTGTCCTGATTAATGGATTATGTAAGAATAAAATGGTGGATGAAGCTGTGAAACTTTTCAAAGAAATGCATTTAAAAAACATCACTCCTAATACTGTTACATACAGTTCACTTATTGATGGTCTTTGCAAATCGGGAAGAATCTCTGATGTTTGGGATCTAATAGATGAAATGCATAACAGAGGCCAACCTGCTGATGTGATCACCTACAATTCTTTATTGGATGCTTTGTGCAAAATCCATCAGGTTGACAAGGCAATTGCGTTGTTAACAAAGATGAAAGACGAAGGAATTCAGCCAGATATGTATACGTACACTATACTTGTTGGTGGACTATGCAAAAATGGAAGACTTAAGGATGCACAAGAGATTTTTCATGATCTTGTGAGAGCTTTTTTTGATGAAGCATTGTCCTTACTCTCACAAATGGAAGACAATGGTTGCACTCCTGATGGTGTAACTTATGAAATTATTATTCGTGCTTTGTTTGAAAGCGACATAAATGATAAGGCGGTGAAACTTCTTCGTGAAATGATTGCAAGAGGTCTACTCTAA

Protein sequence

>MsG0680035264.01.T01

MRKLRYANLSLPTPNSFLLLHRGLHIQPLPSFIDNFDDAVSSFNHILHMKNPTQPIFEFGKILSSLVKLNHFNTVISFSHQMEIKGIQPNIVILNILINSFCHLNQLNFAFSVIAKILKLGFQPDTVTLTTLIKGLCLNGEVRKALYFHDDVIAMGFHLNHVSYGTLINGLCKSGETKAALQVLRKIEEKCVRPDVVMYNTIMDSLCKNKLVIDACDLYSEMLVRRISPDVITYSTLIHGFCIVGQMKEAVGLLNEMTLKNITPNVRTFNTLVDGLCKEGEMKKATSVLPVMIKQGLEPNLVTYTSLMDGYFLVKEVNKANHVFNILSLRGVTPNVHSYSVLINGLCKNKMVDEAVKLFKEMHLKNITPNTVTYSSLIDGLCKSGRISDVWDLIDEMHNRGQPADVITYNSLLDALCKIHQVDKAIALLTKMKDEGIQPDMYTYTILVGGLCKNGRLKDAQEIFHDLVRAFFDEALSLLSQMEDNGCTPDGVTYEIIIRALFESDINDKAVKLLREMIARGLL*