Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035805.01.T01 | XP_003620756.1 | 97.98 | 99 | 2 | 0 | 1 | 99 | 325 | 423 | 2.82E-65 | 213 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035805.01.T01 | G7KQI0 | 97.980 | 99 | 2 | 0 | 1 | 99 | 325 | 423 | 1.34e-65 | 213 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0580026282.01 | MsG0680035805.01 | 0.810667 | 1.028737e-50 | 6.239228e-48 |
| MsG0680035805.01 | MsG0780040118.01 | 0.808004 | 3.831650e-50 | 2.165989e-47 |
| MsG0680035805.01 | MsG0780040119.01 | 0.800176 | 1.629249e-48 | 7.535785e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035805.01.T01 | MTR_6g090060 | 97.980 | 99 | 2 | 0 | 1 | 99 | 325 | 423 | 3.41e-69 | 213 |
| MsG0680035805.01.T01 | MTR_8g102270 | 68.421 | 95 | 24 | 1 | 1 | 95 | 271 | 359 | 7.17e-40 | 135 |
| MsG0680035805.01.T01 | MTR_3g110630 | 67.188 | 64 | 21 | 0 | 30 | 93 | 386 | 449 | 4.40e-18 | 77.8 |
| MsG0680035805.01.T01 | MTR_5g005420 | 71.667 | 60 | 17 | 0 | 31 | 90 | 356 | 415 | 9.06e-17 | 73.9 |
| MsG0680035805.01.T01 | MTR_8g009770 | 43.137 | 102 | 44 | 2 | 1 | 88 | 326 | 427 | 3.98e-14 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680035805.01.T01 | AT4G29310 | 70.000 | 100 | 25 | 2 | 1 | 98 | 327 | 423 | 8.33e-39 | 134 |
| MsG0680035805.01.T01 | AT1G10020 | 76.562 | 64 | 15 | 0 | 30 | 93 | 395 | 458 | 6.33e-23 | 91.7 |
| MsG0680035805.01.T01 | AT3G19680 | 67.164 | 67 | 22 | 0 | 30 | 96 | 422 | 488 | 1.01e-18 | 79.7 |
| MsG0680035805.01.T01 | AT1G50040 | 62.121 | 66 | 25 | 0 | 31 | 96 | 392 | 457 | 3.39e-15 | 69.7 |
| MsG0680035805.01.T01 | AT5G17640 | 44.211 | 95 | 37 | 2 | 1 | 80 | 324 | 417 | 9.27e-12 | 60.1 |
Find 14 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCACAATGAATGTGAAAA+AGG | 0.295332 | 6:+112554463 | None:intergenic |
| TGCTGCTATTGATCTTAGTA+TGG | 0.353674 | 6:+112554676 | MsG0680035805.01.T01:CDS |
| AGTATTATGGATTGTGTTCA+AGG | 0.354254 | 6:+112554522 | MsG0680035805.01.T01:CDS |
| CATTATAATCAATGCAGAAT+TGG | 0.384100 | 6:-112554490 | None:intergenic |
| AATGTTATGAAAGAGTATTA+TGG | 0.388839 | 6:+112554509 | MsG0680035805.01.T01:CDS |
| CAAGGTTACCACCAGGTAAA+GGG | 0.410973 | 6:+112554540 | MsG0680035805.01.T01:CDS |
| TGTGCACCAACTTGCACAAA+AGG | 0.427169 | 6:-112554608 | None:intergenic |
| TCAAGGTTACCACCAGGTAA+AGG | 0.476377 | 6:+112554539 | MsG0680035805.01.T01:CDS |
| GTGATGAGTTCTAGTGTTGA+AGG | 0.486355 | 6:+112554566 | MsG0680035805.01.T01:CDS |
| TGCACAACATGTTACATGTA+TGG | 0.563106 | 6:+112554625 | MsG0680035805.01.T01:CDS |
| CTCATCACAAACCCTTTACC+TGG | 0.570924 | 6:-112554551 | None:intergenic |
| AGTTCTAGTGTTGAAGGTGA+AGG | 0.591958 | 6:+112554572 | MsG0680035805.01.T01:CDS |
| TTGTGTTCAAGGTTACCACC+AGG | 0.654136 | 6:+112554533 | MsG0680035805.01.T01:CDS |
| ATCACAAACCCTTTACCTGG+TGG | 0.684939 | 6:-112554548 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGTTATGAAAGAGTATTA+TGG | + | Chr6:112554509-112554528 | MsG0680035805.01.T01:CDS | 20.0% |
| ! | CATTATAATCAATGCAGAAT+TGG | - | Chr6:112554493-112554512 | None:intergenic | 25.0% |
| ! | AGCTTTTCTCTCATAAACTT+AGG | + | Chr6:112554708-112554727 | MsG0680035805.01.T01:CDS | 30.0% |
| ! | AGTATTATGGATTGTGTTCA+AGG | + | Chr6:112554522-112554541 | MsG0680035805.01.T01:CDS | 30.0% |
| TGCACAACATGTTACATGTA+TGG | + | Chr6:112554625-112554644 | MsG0680035805.01.T01:CDS | 35.0% | |
| TGCTGCTATTGATCTTAGTA+TGG | + | Chr6:112554676-112554695 | MsG0680035805.01.T01:CDS | 35.0% | |
| ! | AGTAAACCTTTTGTGCAAGT+TGG | + | Chr6:112554602-112554621 | MsG0680035805.01.T01:CDS | 35.0% |
| ! | AGTTCTAGTGTTGAAGGTGA+AGG | + | Chr6:112554572-112554591 | MsG0680035805.01.T01:CDS | 40.0% |
| ! | GTGATGAGTTCTAGTGTTGA+AGG | + | Chr6:112554566-112554585 | MsG0680035805.01.T01:CDS | 40.0% |
| ATCACAAACCCTTTACCTGG+TGG | - | Chr6:112554551-112554570 | None:intergenic | 45.0% | |
| CAAGGTTACCACCAGGTAAA+GGG | + | Chr6:112554540-112554559 | MsG0680035805.01.T01:CDS | 45.0% | |
| CTCATCACAAACCCTTTACC+TGG | - | Chr6:112554554-112554573 | None:intergenic | 45.0% | |
| TCAAGGTTACCACCAGGTAA+AGG | + | Chr6:112554539-112554558 | MsG0680035805.01.T01:CDS | 45.0% | |
| TGTGCACCAACTTGCACAAA+AGG | - | Chr6:112554611-112554630 | None:intergenic | 45.0% | |
| TTGTGTTCAAGGTTACCACC+AGG | + | Chr6:112554533-112554552 | MsG0680035805.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 112554470 | 112554769 | 112554470 | ID=MsG0680035805.01;Name=MsG0680035805.01 |
| Chr6 | mRNA | 112554470 | 112554769 | 112554470 | ID=MsG0680035805.01.T01;Parent=MsG0680035805.01;Name=MsG0680035805.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|99 |
| Chr6 | exon | 112554470 | 112554769 | 112554470 | ID=MsG0680035805.01.T01:exon:17756;Parent=MsG0680035805.01.T01 |
| Chr6 | CDS | 112554470 | 112554769 | 112554470 | ID=MsG0680035805.01.T01:cds;Parent=MsG0680035805.01.T01 |
| Gene Sequence |
| Protein sequence |
